14,458 results match your criteria observed cerevisiae

Impact of CO overpressure on yeast mitochondrial associated proteome during the "prise de mousse" of sparkling wine production.

Int J Food Microbiol 2021 May 4;348:109226. Epub 2021 May 4.

Department of Agricultural Chemistry, Edaphology and Microbiology, Severo Ochoa (C6) building, Agrifood Campus of International Excellence CeiA3, University of Cordoba, Ctra. N-IV-A mm 396, 14014 Cordoba, Spain. Electronic address:

The "prise de mousse" stage during sparkling wine elaboration by the traditional method (Champenoise) involves a second fermentation in a sealed bottle followed by a prolonged aging period, known to contribute significantly to the unique organoleptic properties of these wines. During this stage, CO overpressure, nutrient starvation and high ethanol concentrations are stress factors that affect yeast cells viability and metabolism. Since mitochondria are responsible for energy generation and are required for cell aging and response to numerous stresses, we hypothesized that these organelles may play an essential role during the prise de mousse. Read More

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Enhancement of Zn tolerance and accumulation in plants mediated by the expression of Saccharomyces cerevisiae vacuolar transporter ZRC1.

Planta 2021 May 6;253(6):117. Epub 2021 May 6.

Department of Biotechnology, University of Verona, Strada Le Grazie 15, 37134, Verona, Italy.

Main Conclusion: Transgenic Arabidopsis thaliana and Populus alba plants overexpressing the zinc transporter ScZRC1 in shoots exhibit Zn tolerance. Increased Zn concentrations were observed in shoots of P. alba, a species suitable for phytoremediation. Read More

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Photohydroionisation for disinfection of poultry litter.

Br Poult Sci 2021 May 5. Epub 2021 May 5.

Post-Graduation Program in Animal Production and Welfare, Instituto Federal Catarinense, Araquari Campus, Araquari, Santa Catarina, Brazil.

This study evaluated photohydroionisation efficiency on the disinfection of new shavings used as a substrate for litter in poultry industry, pre-inoculated with bacterial, fungal and viral agents.Each replicate consisted of 250 g of new shavings sterilised by autoclaving, challenged with bacterial (, and Abony), fungal () and viral inoculum (Gumboro disease virus). The experiment consisted of six replicates at four times (0, 1, 5 or 10 min exposure) of photohydroionisation. Read More

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The Saccharomyces cerevisiae Ncw2 protein works on the chitin/β-glucan organisation of the cell wall.

Antonie Van Leeuwenhoek 2021 May 4. Epub 2021 May 4.

Laboratory of Microbial Genetics, Department of Genetics, Federal University of Pernambuco, Recife, Brazil.

The NCW2 gene was recently described as encoding a GPI-bounded protein that assists in the re-modelling of the Saccharomyces cerevisiae cell wall (CW) and in the repair of damage caused by the polyhexamethylene biguanide (PHMB) polymer to the cell wall. Its absence produces a re-organization of the CW structure that result in resistance to lysis by glucanase. Hence, the present study aimed to extend the analysis of the Ncw2 protein (Ncw2p) to determine its physiological role in the yeast cell surface. Read More

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Host Chromatin Regulators Required for Cytolethal Distending Toxin Activity in Model.

Infect Immun 2021 May 3. Epub 2021 May 3.

Department of Microbiology and Research Unit on Oral Microbiology and Immunology, Faculty of Dentistry, Chulalongkorn University, Bangkok, Thailand

Cytolethal distending toxin (CDT) is a bacterial genotoxin that causes host cell cycle arrest and death. We previously employed a model with inducible expression of the CDT catalytic subunit from (), CdtB, and showed that a wide variety of host factors play a role in facilitating the activity of CdtB. Our observation that a yeast H2B mutant defective in chromatin condensation was partially resistant to CdtB implies that chromatin structure may affect CDT function. Read More

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Omics analysis reveals mechanism underlying metabolic oscillation during continuous very-high-gravity ethanol fermentation by Saccharomyces cerevisiae.

Biotechnol Bioeng 2021 May 2. Epub 2021 May 2.

State Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic & Developmental Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, China.

During continuous very-high-gravity (VHG) ethanol fermentation with Saccharomyces cerevisiae, the process exhibits sustained oscillation in residual glucose, ethanol and biomass, raising a question: how do yeast cells respond to this phenomenon? In this work, the oscillatory behavior of yeast cells was characterized through transcriptome and metabolome analysis for one complete oscillatory period. By analyzing the accumulation of 26 intracellular metabolites and the expression of 90 genes related to central carbon metabolism and stress response, we confirmed that the process oscillation was attributed to intracellular metabolic oscillation with phase difference, and the expression of HXK1, HXT1,2,4 and PFK1 was significantly different from other genes in the Embden-Meyerhof-Parnas pathway, indicating that glucose transport and phosphorylation could be key nodes for regulating the intracellular metabolism under oscillatory conditions. Moreover, the expression of stress response genes was triggered and affected predominately by ethanol inhibition in yeast cells. Read More

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Complex Mechanisms of Antimony Genotoxicity in Budding Yeast Involves Replication and Topoisomerase I-Associated DNA Lesions, Telomere Dysfunction and Inhibition of DNA Repair.

Int J Mol Sci 2021 Apr 26;22(9). Epub 2021 Apr 26.

Department of Genetics and Cell Physiology, University of Wroclaw, 50-328 Wroclaw, Poland.

Antimony is a toxic metalloid with poorly understood mechanisms of toxicity and uncertain carcinogenic properties. By using a combination of genetic, biochemical and DNA damage assays, we investigated the genotoxic potential of trivalent antimony in the model organism . We found that low doses of Sb(III) generate various forms of DNA damage including replication and topoisomerase I-dependent DNA lesions as well as oxidative stress and replication-independent DNA breaks accompanied by activation of DNA damage checkpoints and formation of recombination repair centers. Read More

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The Mechanisms of Thiosulfate Toxicity against .

Antioxidants (Basel) 2021 Apr 22;10(5). Epub 2021 Apr 22.

State Key Laboratory of Microbial Technology, Shandong University, 72 Binhai Road, Qingdao 266237, China.

Elemental sulfur and sulfite have been used to inhibit the growth of yeasts, but thiosulfate has not been reported to be toxic to yeasts. We observed that thiosulfate was more inhibitory than sulfite to growing in a common yeast medium. At pH < 4, thiosulfate was a source of elemental sulfur and sulfurous acid, and both were highly toxic to the yeast. Read More

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Nonionizing Electromagnetic Field: A Promising Alternative for Growing Control Yeast.

J Fungi (Basel) 2021 Apr 8;7(4). Epub 2021 Apr 8.

Departamento de Ciencia y Tecnología de los Alimentos, Facultad Tecnológica, Universidad de Santiago de Chile, Alameda 3363, Santiago 9170022, Chile.

In the food industry, some fungi are considered to be common spoilage microorganisms which reduce the shelf life of products. To avoid this outcome, different technologies are being developed to control their growth. Electromagnetic fields (EMF) have been used to combat bacterial growth, but there are few studies on yeasts and their possible action mechanisms. Read More

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Pulsed Thermal Method for Monitoring Cell Proliferation in Real-Time.

Sensors (Basel) 2021 Apr 1;21(7). Epub 2021 Apr 1.

Institute for Materials Research (IMO), Hasselt University, 3500 Hasselt, Belgium.

The study of cell proliferation is of great importance for medical and biological research, as well as for industrial applications. To render the proliferation process accurately over time, real-time cell proliferation assay methods are required. This work presents a novel real-time and label-free approach for monitoring cell proliferation by continuously measuring changes in thermal properties that occur at the sensor interface during the process. Read More

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Chemical composition, antioxidant and antimicrobial activity of essential oils from tangerine ( L.), grapefruit ( L.), lemon ( L.) and cinnamon ( Blume).

Z Naturforsch C J Biosci 2021 May 23;76(5-6):175-185. Epub 2020 Nov 23.

Department of Food Technologies, Institute of Food Preservation and Quality-Plovdiv, Agricultural Academy of Bulgaria, 154 Vasil Aprilov Blvd, Plovdiv4000, Bulgaria.

The phytochemical and biological properties of tangerine ( L.), grapefruit ( L.), lemon ( L. Read More

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Assessment of toxicological potential of sodium carboxymethyl beta-glucan, a novel beta-glucan.

Food Chem Toxicol 2021 Apr 24;152:112226. Epub 2021 Apr 24.

Toxi-Coop Zrt., Magyar Jakobinusok Tere 4/B, H-1122, Budapest, Hungary. Electronic address:

In this experimental work, sodium carboxymethyl beta-glucan (CMBG), a chemically altered beta-glucan, is evaluated for mutagenicity and sub-acute oral toxicity. Specifically, the tested material was CM-Glucan Nu, a food grade powder ≥90% CMBG derived from Saccharomyces cerevisiae. A bacterial reverse mutation test was performed and resulted in no mutagenicity. Read More

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Genome-wide toxicogenomic study of the lanthanides sheds light on the selective toxicity mechanisms associated with critical materials.

Proc Natl Acad Sci U S A 2021 May;118(18)

Chemical Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720;

Lanthanides are a series of critical elements widely used in multiple industries, such as optoelectronics and healthcare. Although initially considered to be of low toxicity, concerns have emerged during the last few decades over their impact on human health. The toxicological profile of these metals, however, has been incompletely characterized, with most studies to date solely focusing on one or two elements within the group. Read More

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The OXA2a Insertase of Arabidopsis Is Required for Cytochrome c Maturation.

Plant Physiol 2020 Oct;184(2):1042-1055

Department Biologie I - Botanik, Ludwig-Maximilians-Universität München, Planegg-Martinsried, 82152, Germany.

In yeast (Saccharomyces cerevisiae) and human (Homo sapiens) mitochondria, Oxidase assembly protein1 (Oxa1) is the general insertase for protein insertion from the matrix side into the inner membrane while Cytochrome c oxidase assembly protein18 (Cox18/Oxa2) is specifically involved in the topogenesis of the complex IV subunit, Cox2. Arabidopsis (Arabidopsis thaliana) mitochondria contain four OXA homologs: OXA1a, OXA1b, OXA2a, and OXA2b. OXA2a and OXA2b are unique members of the Oxa1 superfamily, in that they possess a tetratricopeptide repeat (TPR) domain at their C termini. Read More

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October 2020

Alterations of Phagocytic Activity and Capacity in Granulocytes and Monocytes Depend on the Pathogen Strain in Porcine Polytrauma.

Front Med (Lausanne) 2021 6;8:645589. Epub 2021 Apr 6.

Experimental Radiology, Department of Radiology and Nuclear Medicine, Otto von Guericke University, Magdeburg, Germany.

Polytraumatized patients undergo a strong immunological stress upon insult. Phagocytes (granulocytes and monocytes) play a substantial role in immunological defense against bacteria, fungi and yeast, and in the clearance of cellular debris after tissue injury. We have reported a reduced monocytes phagocytic activity early after porcine polytrauma before. Read More

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Transcriptome analysis unveils Gln3 role in amino acids assimilation and fluconazole resistance in .

J Microbiol Biotechnol 2021 Apr 21;31(5). Epub 2021 Apr 21.

División de Biología Molecular, Instituto Potosino de Investigación Científica y Tecnológica A. C. (IPICYT).

After , is one the most common fungal species associated with candidemia in nosocomial infections. Rapid acquisition of nutrients from the host is important for pathogen survival, which possess metabolic flexibility to assimilate different carbon and nitrogen compounds. In , nitrogen assimilation is controlled through a mechanism known as Nitrogen Catabolite Repression (NCR). Read More

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Removal of the fishy malodor from Bangia fusco-purpurea via fermentation of Saccharomyces cerevisiae, Acetobacter pasteurianus, and Lactobacillus plantarum.

J Food Biochem 2021 Apr 20:e13728. Epub 2021 Apr 20.

College of Food and Biological Engineering, Jimei University, Xiamen, China.

The present study aims to evaluate the deodorization of Bangia fusco-purpurea using microorganism fermentation with commercial starter cultures of Saccharomyces cerevisiae, Acetobacter pasteurianus, and Lactobacillus plantarum. The results showed the fermentation with S. cerevisiae, A. Read More

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Effects of bacterial cultures, enzymes, and yeast-based feed additive combinations on ruminal fermentation in a dual-flow continuous culture system.

Transl Anim Sci 2021 Apr 11;5(2):txab026. Epub 2021 Feb 11.

Department of Animal Sciences, University of Florida, Gainesville, FL 32611, USA.

Bacterial cultures, enzymes, and yeast-derived feed additives are often included in commercial dairy rations due to their effects on ruminal fermentation. However, the effects of these additives when fed together are not well understood. The objective of this study was to evaluate the changes in ruminal fermentation when a dairy ration is supplemented with combinations of bacterial probiotics, enzymes and yeast. Read More

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The RSC complex remodels nucleosomes in transcribed coding sequences and promotes transcription in Saccharomyces cerevisiae.

Genetics 2021 Apr;217(4)

Department of Biological Sciences, Oakland University, Rochester, MI 48309, USA.

RSC (Remodels the Structure of Chromatin) is a conserved ATP-dependent chromatin remodeling complex that regulates many biological processes, including transcription by RNA polymerase II (Pol II). We report that RSC contributes in generating accessible nucleosomes in transcribed coding sequences (CDSs). RSC MNase ChIP-seq data revealed that RSC-bound nucleosome fragments were very heterogenous (∼80 bp to 180 bp) compared to a sharper profile displayed by the MNase inputs (140 bp to 160 bp), supporting the idea that RSC promotes accessibility of nucleosomal DNA. Read More

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Innate immunity to yeast prions: Btn2p and Cur1p curing of the [URE3] prion is prevented by 60S ribosomal protein deficiency or ubiquitin/proteasome system overactivity.

Genetics 2021 Apr;217(4)

Laboratory of Biochemistry and Genetics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892-0830, USA.

[URE3] is an amyloid-based prion of Ure2p, a negative regulator of poor nitrogen source catabolism in Saccharomyces cerevisiae. Overproduced Btn2p or its paralog Cur1p, in processes requiring Hsp42, cure the [URE3] prion. Btn2p cures by collecting Ure2p amyloid filaments at one place in the cell. Read More

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Sonication treatment of pomegranate juice containing and : Thermodynamic and predictive modeling after treatment and during shelf life.

Food Sci Technol Int 2021 Apr 14:10820132211009442. Epub 2021 Apr 14.

Department of Mechanical Engineering, Fasa University, Fasa, Iran.

The effect of ultrasound treatment (100 W, 30 kHz; 50 and 100% amplitudes) on inactivation of and in pomegranate juice and shelf life of the juice during storage at different temperatures (5, 15 and 30 °C) for 30 days was modeled using predictive and thermodynamics models. The Baranyi and square root type models were implemented to determine the growth rate of microorganisms. It was predicted that the minimum growth temperature () of increased by increasing sonication amplitude, however, the of was not function of sonication amplitude. Read More

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Sterol uptake analysis in Saccharomyces and non-Saccharomyces wine yeast species.

FEMS Yeast Res 2021 Apr;21(3)

SPO, Univ Montpellier, INRAE, Institut Agro, 2, place Pierre Viala, 34060 Montpellier, France.

Sterols are essential components of the yeast membrane and their synthesis requires oxygen. Yet, Saccharomyces cerevisiae has developed the ability to take up sterols from the medium under anaerobiosis. Here we investigated sterol uptake efficiency and the expression of genes related to sterol import in Saccharomyces and non-Saccharomyces wine yeast species fermenting under anaerobic conditions. Read More

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Plasmodium falciparum Atg18 localizes to the food vacuole via interaction with the multi-drug resistance protein 1 and phosphatidylinositol 3-phosphate.

Biochem J 2021 May;478(9):1705-1732

CSIR-Centre for Cellular and Molecular Biology, Hyderabad 500007, TS, India.

Autophagy, a lysosome-dependent degradative process, does not appear to be a major degradative process in malaria parasites and has a limited repertoire of genes. To better understand the autophagy process, we investigated Plasmodium falciparum Atg18 (PfAtg18), a PROPPIN family protein, whose members like S. cerevisiae Atg18 (ScAtg18) and human WIPI2 bind PI3P and play an essential role in autophagosome formation. Read More

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Substitution of histone H3 arginine 72 to alanine leads to deregulation of isoleucine biosynthesis in budding yeast Saccharomyces cerevisiae.

Biochem Cell Biol 2021 Apr 11. Epub 2021 Apr 11.

IISER Bhopal, 189785, Bhopal, India, 462066;

Histone residues play an essential role in the regulation of various biological processes. In the present study, we have utilized the H3/H4 histone mutant library to probe functional aspects of histone residues in amino acid biosynthesis. We found that histone residue H3R72 plays a crucial role in the regulation of isoleucine biosynthesis. Read More

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Loss of Smi1, a protein involved in cell wall synthesis, extends replicative life span by enhancing rDNA stability in Saccharomyces cerevisiae.

J Biol Chem 2021 Jan 7;296:100258. Epub 2021 Jan 7.

School of Biological Sciences, Seoul National University, Seoul, Republic of Korea; Institute of Microbiology, Seoul National University, Seoul, Republic of Korea. Electronic address:

In Saccharomyces cerevisiae, replicative life span (RLS) is primarily affected by the stability of ribosomal DNA (rDNA). The stability of the highly repetitive rDNA array is maintained through transcriptional silencing by the NAD-dependent histone deacetylase Sir2. Recently, the loss of Smi1, a protein of unknown molecular function that has been proposed to be involved in cell wall synthesis, has been demonstrated to extend RLS in S. Read More

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January 2021

Toxicological evaluation of a glycan preparation from an enzymatic hydrolysis of Saccharomyces cerevisiae.

Regul Toxicol Pharmacol 2021 Apr 5;123:104924. Epub 2021 Apr 5.

Alltech France SARL, Rue Charles Amand, 14500, Vire, France.

The aim of this paper was to provide a comprehensive toxicological and safety evaluation of a yeast cell wall preparation (YCWP) for use as an animal feed ingredient. The following toxicological assessments were carried out: the mutagenic activity was tested using the Ames' Test in five Salmonella typhimurium strains; clastogenic activity was investigated using the mammalian micronucleus test in Swiss ICO OF1 (IOPS Caw) mice; genotoxic activity was assessed using the in vitro mammalian chromosomal aberration test in human lymphocytes; acute oral toxicity was tested by administration of a single dose of 2000 mg/kg BW. Eye and skin irritation were assessed in rabbits according to OECD guidelines; skin sensitivity was established in guinea pigs by means of the Buehler test, while acute dermal and inhalation studies in rats were further completed, also according to OECD guidelines. Read More

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Cell volume homeostatically controls the rDNA repeat copy number and rRNA synthesis rate in yeast.

PLoS Genet 2021 Apr 7;17(4):e1009520. Epub 2021 Apr 7.

Instituto de Biotecnología y Biomedicina (Biotecmed), Universitat de València, Burjassot, Spain.

The adjustment of transcription and translation rates to the changing needs of cells is of utmost importance for their fitness and survival. We have previously shown that the global transcription rate for RNA polymerase II in budding yeast Saccharomyces cerevisiae is regulated in relation to cell volume. Total mRNA concentration is constant with cell volume since global RNApol II-dependent nascent transcription rate (nTR) also keeps constant but mRNA stability increases with cell size. Read More

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Coevolution of ribosomal RNA expansion segment 7L and assembly factor Noc2p specializes the ribosome biogenesis pathway between Saccharomyces cerevisiae and Candida albicans.

Nucleic Acids Res 2021 May;49(8):4655-4667

School of Ecology and Environment, Northwestern Polytechnical University, Xi'an 710129, China.

Ribosomes of different species share an evolutionarily conserved core, exhibiting flexible shells formed partially by the addition of species-specific ribosomal RNAs (rRNAs) with largely unexplored functions. In this study, we showed that by swapping the Saccharomyces cerevisiae 25S rRNA genes with non-S. cerevisiae homologs, species-specific rRNA variations caused moderate to severe pre-rRNA processing defects. Read More

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Aeration mitigates endoplasmic reticulum stress in even without mitochondrial respiration.

Microb Cell 2021 Mar 31;8(4):77-86. Epub 2021 Mar 31.

Graduate School of Science and Technology, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, Nara, 630-0192, Japan.

is a facultative anaerobic organism that grows well under both aerobic and hypoxic conditions in media containing abundant fermentable nutrients such as glucose. In order to deeply understand the physiological dependence of on aeration, we checked endoplasmic reticulum (ER)-stress status by monitoring the splicing of mRNA, which is promoted by the ER stress-sensor protein, Ire1. -mRNA splicing that was caused by conventional ER-stressing agents, including low concentrations of dithiothreitol (DTT), was more potent in hypoxic cultures than in aerated cultures. Read More

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Use of non-Saccharomyces yeasts in cider fermentation: Importance of the nutrients addition to obtain an efficient fermentation.

Int J Food Microbiol 2021 Mar 17;347:109169. Epub 2021 Mar 17.

Biotecnología Industrial, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco A.C., Camino Arenero 1227, Col. El Bajío del Arenal, C.P. 45019 Zapopan, Jalisco, Mexico. Electronic address:

The isolation of autochthonous yeast species presents a good strategy to select new microorganisms for developing an adequate inoculum to carry out fermentations and generate representative products of the cider production zone. However, non-Saccharomyces yeasts have been considered to have low capacity to carry out a complete fermentation as Saccharomyces cerevisiae. In this work, five autochthonous yeasts from a cider fermentation process were isolated and identified as Saccharomyces cerevisiae, Kluyveromyces marxianus, Pichia membranaefaciens, P. Read More

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