182 results match your criteria numbers cultivable

Effect of Cyberlindnera jadinii yeast as a protein source on intestinal microbiota and butyrate levels in post-weaning piglets.

Anim Microbiome 2020 May 5;2(1):13. Epub 2020 May 5.

Department of Animal and Aquacultural Sciences, Faculty of Biosciences, Norwegian University of Life Sciences, Ås, Norway.

Background: Dietary yeast inclusions in a pig diet may drive changes both in gut bacterial composition and bacterial functional profile. This study investigated the effect of Cyberlindnera jadinii as a protein to replace 40% of the conventional proteins in a diet for weanling pigs on the microbiota in the small and large intestine, colonic short-chain fatty acid concentration, and colonic histopathology parameters. Seventy-two pigs weaned at 28 days of age were randomly assigned to either a control or a C. Read More

View Article and Full-Text PDF

Do Differences in Cultivable Subgingival Species Exist between Different Periodontitis Stages and Grades?

Oral Health Prev Dent 2021 ;19(1):15-24

Purpose: To investigate the subgingival microbiological profiles of patients with periodontitis, to determine their stage and grade scores and to evaluate the differences in the microbiota among different stages and grades. Materials and Methods: Sixty-seven (n = 67) periodontitis patients were selected. Periodontitis staging and grading, following the 2018 classification system, were defined. Read More

View Article and Full-Text PDF
January 2021

Evaluation of potential impacts on biodiversity of the salt-tolerant transgenic Eucalyptus camaldulensis harboring an RNA chaperonic RNA-Binding-Protein gene derived from common ice plant.

Transgenic Res 2021 Feb 21;30(1):23-34. Epub 2021 Jan 21.

Tsukuba Plant Innovation Research Center, University of Tsukuba, Gene Research Center Bldg., Ten-nodai, Tsukuba, Ibaraki, 305-8572, Japan.

We recently reported that a genetic transformation of the RNA-Binding-Protein (McRBP), an RNA chaperone gene derived from common ice plant (Mesembryanthemum crystallinum), alleviated injury and loss of biomass production by salt stress in Eucalyptus camaldulensis in a semi-confined screen house trial. In this study, we assessed the potential environmental impact of the transgenic Eucalyptus in a manner complying with Japanese biosafety regulatory framework required for getting permission for experimental confined field trials. Two kinds of bioassays for the effects of allelopathic activity on the growth of other plants, i. Read More

View Article and Full-Text PDF
February 2021

Rhizobacteria and Arbuscular Mycorrhizal Fungi of Oil Crops (Physic Nut and Sacha Inchi): A Cultivable-Based Assessment for Abundance, Diversity, and Plant Growth-Promoting Potentials.

Plants (Basel) 2020 Dec 14;9(12). Epub 2020 Dec 14.

Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand.

Nowadays, oil crops are very attractive both for human consumption and biodiesel production; however, little is known about their commensal rhizosphere microbes. In this study, rhizosphere samples were collected from physic nut and sacha inchi plants grown in several areas of Thailand. Rhizobacteria, cultivable in nitrogen-free media, and arbuscular mycorrhizal (AM) fungi were isolated and examined for abundance, diversity, and plant growth-promoting activities (indole-3-acetic acid (IAA) and siderophore production, nitrogen fixation, and phosphate solubilization). Read More

View Article and Full-Text PDF
December 2020

Influence of ventilation use and occupant behaviour on surface microorganisms in contemporary social housing.

Sci Rep 2020 07 16;10(1):11841. Epub 2020 Jul 16.

Water, Public Health and Environmental Engineering Group, School of Civil Engineering, University of Leeds, Leeds, UK.

In the context of increasingly airtight homes, there is currently little known about the type and diversity of microorganisms in the home, or factors that could affect their abundance, diversity and nature. In this study, we examined the type and prevalence of cultivable microorganisms at eight different sites in 100 homes of older adults located in Glasgow, Scotland. The microbiological sampling was undertaken alongside a household survey that collated information on household demographics, occupant behaviour, building characteristics, antibiotic use and general health information. Read More

View Article and Full-Text PDF

Multimodal Approach to Assessment of Fecal Microbiota Donors based on Three Complementary Methods.

J Clin Med 2020 Jun 29;9(7). Epub 2020 Jun 29.

Department of Hematology, Oncology and Internal Medicine, Medical University of Warsaw, 02-091 Warsaw, Poland.

Methods of stool assessment are mostly focused on next-generation sequencing (NGS) or classical culturing, but only rarely both. We conducted a series of experiments using a multi-method approach to trace the stability of gut microbiota in various donors over time, to find the best method for the proper selection of fecal donors and to find "super-donor" indicators. Ten consecutive stools donated by each of three donors were used for the experiments (30 stools in total). Read More

View Article and Full-Text PDF

The incidence of anaerobic bacteria in adult patients with chronic sinusitis: A prospective, single-centre microbiological study.

Eur J Microbiol Immunol (Bp) 2020 Jun 5. Epub 2020 Jun 5.

4 Department of Oto-Rhino-Laryngology and Head-, Neck Surgery, Faculty of Medicine, University of Szeged, Szeged, Hungary.

Introduction: Chronic sinusitis caused by anaerobes is a particular concern clinically, because many of the complications are associated with infections caused by these organisms. The aim of this study was to evaluate the incidence of anaerobic bacteria in chronic sinusitis in adults as a part of a prospective microbiological study.

Materials And Methods: Over a one-year period, aspirations of maxillary sinus secretions and/or ethmoid cavities were derived in n = 79 adult patients with chronic sinusitis by endoscopy in a tertiary-care teaching hospital in Hungary. Read More

View Article and Full-Text PDF

Intravaginal administration of gelatine capsules containing freeze-dried autochthonous lactobacilli: a double-blind, randomised clinical trial of safety.

Benef Microbes 2020 Feb 17;11(1):5-17. Epub 2019 Dec 17.

Centro de Referencia para Lactobacilos (CERELA)-CONICET, Chacabuco 145, 4000 San Miguel de Tucumán, Tucumán, Argentina.

Vaginal lactobacilli (LAB) in probiotic formulas constitute a promising alternative for microbiome reconstitution and for the prevention and treatment of urogenital infections. A double-blind, randomised clinical trial was conducted to assess the safety of LAB-gelatine capsules vaginally administered to healthy sexually active women. Participants were randomised into three groups: intervention A: CRL1324, CRL1263 and CRL1307; intervention B: CRL1332, CRL1256 and CRL1320; and intervention C: placebo. Read More

View Article and Full-Text PDF
February 2020

Potential for residual contamination by Streptococcus equi subspp equi of endoscopes and twitches used in diagnosis of carriers of strangles.

Equine Vet J 2020 Nov 2;52(6):884-890. Epub 2020 Mar 2.

Department of Clinical Sciences, Swedish University of Agricultural Sciences, Uppsala, Sweden.

Background: Endoscopic examinations are essential for diagnosis and treatment of strangles (S equi infection) in horses. However, even after disinfection, endoscopes may retain viable bacteria or bacterial DNA. Twitches are commonly used during endoscopic examinations and can thus also potentially transmit the organism to other horses. Read More

View Article and Full-Text PDF
November 2020

Isolation of bacteria at different points of Pleurotus ostreatus cultivation and their influence in mycelial growth.

Microbiol Res 2019 Dec 13;234:126393. Epub 2019 Dec 13.

Institute of Applied Microbiology, IFZ, Justus-Liebig University Giessen, 35392, Giessen, Germany.

Pleurotus ostreatus is one of the most cultivated edible mushrooms worldwide and few approaches have been done to analyze bacterial influence during its cultivation. Therefore, bacteria from commercial spawn, mycelial-colonized straw and fruiting bodies from healthy productive samples were counted, isolated and tested for their mycelial growth promoting ability. Bacterial cell numbers at different steps of the process showed low bacterial cell numbers in spawn and in fruiting bodies inner tissue compared to the high concentration in mycelial-colonized straw. Read More

View Article and Full-Text PDF
December 2019

Cultivable bacterial diversity associated with bromeliad roots from ironstone outcrops in central Brazil.

Braz J Biol 2020 Oct-Dec;80(4):872-880

Ciências Biológicas, Laboratório de Microbiologia, Universidade Federal de Mato Grosso do Sul - UFMS, do Pantanal, Av. Rio Branco, 1.270, Bairro Universitário, CEP 79304-902, Corumbá, MS, Brasil.

Studies on the bacterial diversity associated with wild plants are rare, especially on those that grow in association with bromeliads. In the present study, we isolated and identified epiphytic and endophytic bacteria from the roots of the bromeliads Dyckia excelsa, Dyckia leptostachya and Deuterocohnia meziana occurring in the "cangas" in the Pantanal from Mato Grosso do Sul State, Brazil. The epiphytic bacteria were isolated from washed roots, while the endophytic bacteria were isolated from surface disinfested roots. Read More

View Article and Full-Text PDF
December 2020

The plant organs and rhizosphere determine the common bean mycobiome.

Braz J Microbiol 2020 Jun 2;51(2):765-772. Epub 2020 Jan 2.

Departamento de Microbiologia/ Instituto de Biotecnologia Aplicada à Agropecuária (BIOAGRO), Universidade Federal de Viçosa, Viçosa, MG, Brazil.

The plant microbiota diversity is often underestimated when approaches developed mainly for the identification of cultivable microorganisms are used. High-throughput sequencing allows a deeper understanding of the microbial diversity associated with plants. The amplification of ITS1 was used to analyze fungal diversity in several plant organs and rhizosphere of three common bean (Phaseolus vulgaris) varieties grown in a greenhouse. Read More

View Article and Full-Text PDF

Uncovering Akkermansia muciniphila resilience or susceptibility to different temperatures, atmospheres and gastrointestinal conditions.

Anaerobe 2020 Feb 14;61:102135. Epub 2019 Dec 14.

Universidade Católica Portuguesa, CBQF - Centro de Biotecnologia e Química Fina - Laboratório Associado, Escola Superior de Biotecnologia, Rua Diogo Botelho 1327, 4169-005 Porto, Portugal.

Data regarding Akkermansia muciniphila viability under stress remains scarce despite its beneficial potential. Therefore, the main goal was to assess A. muciniphila culturability when exposed to different temperatures, atmospheres and gastrointestinal simulated conditions. Read More

View Article and Full-Text PDF
February 2020

Inhibitory Effect of 5-Aminoimidazole-4-Carbohydrazonamides Derivatives Against Candida spp. Biofilm on Nanohydroxyapatite Substrate.

Mycopathologia 2019 Dec 2;184(6):775-786. Epub 2019 Nov 2.

Health Sciences Faculty, University Fernando Pessoa, Porto, Portugal.

Candida can adhere and form biofilm on biomaterials commonly used in medical devices which is a key attribute that enhances its ability to cause infections in humans. Furthermore, biomaterial-related infections represent a major therapeutic challenge since Candida biofilms are implicated in antifungal therapies failure. The goals of the present work were to investigate the effect of three 5-aminoimidazole-4-carbohydrazonamides, namely (Z)-5-amino-1-methyl-N'-aryl-1H-imidazole-4-carbohydrazonamides [aryl = phenyl (1a), 4-fluorophenyl (1b), 3-fluorophenyl (1c)], on Candida albicans and Candida krusei biofilm on nanohydroxyapatite substrate, a well-known bioactive ceramic material. Read More

View Article and Full-Text PDF
December 2019

Metagenomics analysis of bacterial structure communities within natural biofilm.

Heliyon 2019 Aug 23;5(8):e02271. Epub 2019 Aug 23.

Environmental Microbiology Lab., Water Pollution Research Department, National Research Centre, Dokki, 12622, Giza, Egypt.

The bacterial profiles of natural household biofilm have not been widely investigated. The majorities of these bacterial lineages are not cultivable. Thus, this study aims (i) to enumerate some potential bacterial lineages using culture based method within biofilm samples and confirmed using Biolog GEN III and polymerase chain reaction (PCR). Read More

View Article and Full-Text PDF

L. as an underutilized saline-tolerant plant inhabited by endophytic diazotrophs.

J Adv Res 2019 Sep 16;19:49-56. Epub 2019 May 16.

Leibniz Institute of Vegetable- and Ornamental Crops, Theodor-Echtermeyer-Weg 1, D-14979 Grossbeeren, Germany.

Despite the great interest in using halophyte L. as a crop in extreme saline habitats, little is known about the role played by associated endophytic bacteria in increasing tolerance of the host-plant to nutrient deficiency. Main objectives of this study were to investigate the community composition of diazotrophic endophytes of grown under natural conditions, and determine the proportion of plant-growth promoting bacterial strains able to fix N. Read More

View Article and Full-Text PDF
September 2019

Characterization of the total and viable bacterial and fungal communities associated with the International Space Station surfaces.

Microbiome 2019 04 8;7(1):50. Epub 2019 Apr 8.

Jet Propulsion Laboratory, California Institute of Technology, Biotechnology and Planetary Protection Group,, Pasadena, CA, USA.

Background: The International Space Station (ISS) is a closed system inhabited by microorganisms originating from life support systems, cargo, and crew that are exposed to unique selective pressures such as microgravity. To date, mandatory microbial monitoring and observational studies of spacecraft and space stations have been conducted by traditional culture methods, although it is known that many microbes cannot be cultured with standard techniques. To fully appreciate the true number and diversity of microbes that survive in the ISS, molecular and culture-based methods were used to assess microbial communities on ISS surfaces. Read More

View Article and Full-Text PDF

Extraction of Biofilms From Ureteral Stents for Quantification and Cultivation-Dependent and -Independent Analyses.

Front Microbiol 2018 10;9:1470. Epub 2018 Jul 10.

Laboratory for Biointerfaces, Empa, Swiss Federal Laboratories for Materials Science and Technology, St. Gallen, Switzerland.

Ureteral stenting is a common surgical procedure, which is associated with a high morbidity and economic burden, but the knowledge on the link between biofilms on these stents, morbidity, and the impact of the involved microbiota is still limited. This is partially due to a lack of methods that allow for a controlled extraction of the biofilms from stents. Development of an appropriate model to assess prevention of biofilm formation by antimicrobial coatings and biomaterials requires a profound understanding of the biofilm composition, including the involved microbiota. Read More

View Article and Full-Text PDF

Analysis of Bacterial Diversity in Different Heavy Oil Wells of a Reservoir in South Oman with Alkaline pH.

Scientifica (Cairo) 2018 21;2018:9230143. Epub 2018 Mar 21.

Department of Biology, College of Science, Sultan Qaboos University, Muscat, Oman.

The identification of potential hydrocarbon utilizing bacteria is an essential requirement in microbial enhanced oil recovery (MEOR). Molecular approaches like proteomic and genomic characterization of the isolates are replacing the traditional method of identification with systemic classification. Genotypic profiling of the isolates includes fingerprint or pattern-based technique and sequence-based technique. Read More

View Article and Full-Text PDF

The cultivable bacterial flora of the esophagus in subjects with esophagitis.

Scand J Gastroenterol 2018 06 4;53(6):650-656. Epub 2018 Apr 4.

c Department of ENT and Maxillofacial Surgery , NÄL Medical Centre Hospital , Trollhättan , Sweden.

Background: The healthy human esophagus is colonized by bacteria similar to that of the oral mucosa. However, little is known about the microbiome of the esophagus in esophagitis or the possible role of bacteria in the inflammatory response.

Aim: To survey bacterial diversity and compare the microbiome of the esophagus in subjects with gastro-esophageal reflux disease (GERD) and eosinophilic esophagitis (EoE). Read More

View Article and Full-Text PDF

Effect of disinfectant residual on the interaction between bacterial growth and assimilable organic carbon in a drinking water distribution system.

Chemosphere 2018 Jul 9;202:586-597. Epub 2018 Mar 9.

College of Environmental Science and Engineering, Tongji University, Shanghai, 200092, China.

Public health is threatened by deteriorated water quality due to bacterial regrowth and uncontrolled growth-related problems in drinking water distribution systems (DWDSs). To investigate the scope of this problem, a two-year field study was conducted in south China. The amount of assimilable organic carbon (AOC), total cell concentrations (TCC), and intact cell concentrations (ICC) of water samples were determined by flow cytometry. Read More

View Article and Full-Text PDF

Droplet digital PCR improves absolute quantification of viable lactic acid bacteria in faecal samples.

J Microbiol Methods 2018 05 14;148:64-73. Epub 2018 Mar 14.

Danone Research Centre Daniel Carasso, RD 128 Avenue de la Vauve, 91700 Palaiseau Cedex, France. Electronic address:

Analysing correlations between the observed health effects of ingested probiotics and their survival in digestive tract allows adapting their preparations for food. Tracking ingested probiotic in faecal samples requires accurate and specific tools to quantify live vs dead cells at strain level. Traditional culture-based methods are simpler to use but they do not allow quantifying viable but non-cultivable (VBNC) cells and they are poorly discriminant below the species level. Read More

View Article and Full-Text PDF

Glyphosate application increased catabolic activity of gram-negative bacteria but impaired soil fungal community.

Environ Sci Pollut Res Int 2018 May 14;25(15):14762-14772. Epub 2018 Mar 14.

NJU-NJFU Institute of Plant Molecular Biology, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, 210023, China.

Glyphosate is a non-selective organophosphate herbicide that is widely used in agriculture, but its effects on soil microbial communities are highly variable and often contradictory, especially for high dose applications. We applied glyphosate at two rates: the recommended rate of 50 mg active ingredient kg soil and 10-fold this rate to simulate multiple glyphosate applications during a growing season. After 6 months, we investigated the effects on the composition of soil microbial community, the catabolic activity and the genetic diversity of the bacterial community using phospholipid fatty acids (PLFAs), community level catabolic profiles (CLCPs), and 16S rRNA denaturing gradient gel electrophoresis (DGGE). Read More

View Article and Full-Text PDF

Hunting for cultivable Micromonospora strains in soils of the Atacama Desert.

Antonie Van Leeuwenhoek 2018 Aug 26;111(8):1375-1387. Epub 2018 Feb 26.

School of Natural and Environmental Sciences, Newcastle University, Newcastle upon Tyne, UK.

Innovative procedures were used to selectively isolate small numbers of Micromonospora strains from extreme hyper-arid and high altitude Atacama Desert soils. Micromonosporae were recognised on isolation plates by their ability to produce filamentous microcolonies that were strongly attached to the agar. Most of the isolates formed characteristic orange colonies that lacked aerial hyphae and turned black on spore formation, whereas those from the high altitude soil were dry, blue-green and covered by white aerial hyphae. Read More

View Article and Full-Text PDF

Sewage sludge amendment and inoculation with plant-parasitic nematodes do not facilitate the internalization of Salmonella Typhimurium LT2 in lettuce plants.

Food Microbiol 2018 May 13;71:111-119. Epub 2017 Jun 13.

Julius Kühn-Institut, Federal Research Centre for Cultivated Plants (JKI), Institute for Epidemiology and Pathogen Diagnostics, Braunschweig, Germany. Electronic address:

Contamination of fruits and vegetables with Salmonella is a serious threat to human health. In order to prevent possible contaminations of fresh produce it is necessary to identify the contributing ecological factors. In this study we investigated whether the addition of sewage sludge or the presence of plant-parasitic nematodes foster the internalization of Salmonella enterica serovar Typhimurium LT2 into lettuce plants, posing a potential threat for human health. Read More

View Article and Full-Text PDF

Using RT qPCR for Quantifying Mycobacteria marinum from In Vitro and In Vivo Samples.

Methods Mol Biol 2018 ;1736:137-145

School of Medicine, University of St Andrews, St Andrews, UK.

Mycobacterium marinum, the causative agent of fish tuberculosis, is rarely a human pathogen causing a chronic skin infection. It is now wildely used as a model system in animal models, especially in zebra fish model, to study the pathology of tuberculosis and as a means of screening new anti-tuberculosis agent. To facilitate such research, quantifying the viable count of M. Read More

View Article and Full-Text PDF
December 2018

Experimental human-like model to assess the part of viable Legionella reaching the thoracic region after nebulization.

PLoS One 2017 5;12(10):e0186042. Epub 2017 Oct 5.

University of Lyon, UJM-Saint-Etienne, CNRS, EVS-ISTHME UMR 5600, Saint-Etienne, France.

The incidence of Legionnaires' disease (LD) in European countries and the USA has been constantly increasing since 1998. Infection of humans occurs through aerosol inhalation. To bridge the existing gap between the concentration of Legionella in a water network and the deposition of bacteria within the thoracic region (assessment of the number of viable Legionella), we validated a model mimicking realistic exposure through the use of (i) recent technology for aerosol generation and (ii) a 3D replicate of the human upper respiratory tract. Read More

View Article and Full-Text PDF
November 2017

Effect of biosynthesized silver nanoparticles on native soil microflora via plant transport during plant-pathogen-nanoparticles interaction.

3 Biotech 2017 Oct 23;7(5):345. Epub 2017 Sep 23.

Division of Plant Microbe Interactions, CSIR-National Botanical Research Institute, Rana Pratap Marg, Lucknow, 226 001 India.

In this study, the interaction of biosynthesized silver nanoparticles (BSNP) with native soil via plant transport was assessed in model pathosystem of and . Foliar application of 5 μg/mL of BSNP reduced number of spores of fungi to 2.2 × 10 from 7 × 10, while numbers of lesions got reduced to 0. Read More

View Article and Full-Text PDF
October 2017

Quantifying the combined effects of pronase and benzalkonium chloride in removing late-stage Listeria monocytogenes-Escherichia coli dual-species biofilms.

Biofouling 2017 09;33(8):690-702

a Department of Microbiology and Technology of Marine Products , Instituto de Investigaciones Marinas (IIM-CSIC) , Pontevedra , Spain.

This work presents the assessment of the effectivity of a pronase (PRN)-benzalkonium chloride (BAC) sequential treatment in removing Listeria monocytogenes-Escherichia coli dual-species biofilms grown on stainless steel (SS) using fluorescence microscopy and plate count assays. The effects of PRN-BAC on the occupied area (OA) by undamaged cells in 168 h dual-species samples were determined using a first-order factorial design. Empirical equations significantly (r = 0. Read More

View Article and Full-Text PDF
September 2017

Plant-fed versus chemicals-fed rhizobacteria of Lucerne: Plant-only teabags culture media not only increase culturability of rhizobacteria but also recover a previously uncultured Lysobacter sp., Novosphingobium sp. and Pedobacter sp.

PLoS One 2017 7;12(7):e0180424. Epub 2017 Jul 7.

Leibniz Institute of Vegetable and Ornamental Crops Großbeeren/ Erfurt e.V. (IGZ), Großbeeren, Germany.

In an effort to axenically culture the previously uncultivable populations of the rhizobacteria of Lucerne (Medicago sativa L.), we propose plant-only teabags culture media to mimic the nutritional matrix available in the rhizosphere. Here, we show that culture media prepared from Lucerne powder teabags substantially increased the cultivability of Lucerne rhizobacteria compared with a standard nutrient agar, where we found that the cultivable populations significantly increased by up to 60% of the total bacterial numbers as estimated by Quantitative Real-time Polymerase Chain Reaction (qRT-PCR). Read More

View Article and Full-Text PDF
October 2017