69,393 results match your criteria nuclear membrane


Isoquercitrin protects HUVECs against high glucose‑induced apoptosis through regulating p53 proteasomal degradation.

Int J Mol Med 2021 Jul 13;48(1). Epub 2021 May 13.

Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, P.R. China.

High glucose (HG)‑induced endothelial apoptosis serves an important role in the vascular dysfunction associated with diabetes mellitus (DM). It has been reported that isoquercitrin (IQC), a flavonoid glucoside, possesses an anti‑DM effect, but the mechanism requires further investigation. The present study investigated the effect of IQC against HG‑induced apoptosis in human umbilical vein endothelial cells (HUVECs) and explored its molecular mechanism. Read More

View Article and Full-Text PDF

Electrostatic modulation of hnRNPA1 low-complexity domain liquid-liquid phase separation and aggregation.

Protein Sci 2021 May 12. Epub 2021 May 12.

Department of Pharmacology and Chemical Biology, Baylor College of Medicine, Houston, TX, USA.

Membrane-less organelles and RNP granules are enriched in RNA and RNA-binding proteins containing disordered regions. Heterogeneous nuclear ribonucleoprotein A1 (hnRNPA1), a key regulating protein in RNA metabolism, localizes to cytoplasmic RNP granules including stress granules. Dysfunctional nuclear-cytoplasmic transport and dynamic phase separation of hnRNPA1 leads to abnormal amyloid aggregation and neurodegeneration. Read More

View Article and Full-Text PDF

Faecal proteome in clinically healthy dogs and cats: Findings in pooled faeces from 10 cats and 10 dogs.

Vet Rec Open 2021 Dec 7;8(1):e9. Epub 2021 Apr 7.

School of Biosciences and Veterinary Medicine University of Camerino Matelica MC Italy.

Background: In the scientific literature, there are only a few manuscripts available on small animal faecal proteomics.

Methods: In the present pilot study, this evaluation was performed using pooled faecal samples from 10 clinically healthy dogs and, for the first time, in 10 clinically healthy cats by mean of two-dimensional electrophoresis followed by liquid chromatography-tandem mass spectrometry.

Results: Our results showed the presence of nine (albumin, alkaline phosphatase, chymotrypsin-C-like, cytosol aminopeptidase, elastase-3B/proteinase E, immunoglobulins and nuclear pore membrane glycoprotein 210) and 14 (albumin, caspase recruitment domain-containing protein, chymotrypsin-like, deleted in malignant brain tumours 1 protein-like, hypothetical protein LOC107375, immunoglobulin, kallikrein-1, superoxide dismutase, transthyretin precursor, interstitial collagenase-like) different proteins in canine and feline faeces, respectively. Read More

View Article and Full-Text PDF
December 2021

A human infertility-associated KASH5 variant promotes mitochondrial localization.

Sci Rep 2021 May 12;11(1):10133. Epub 2021 May 12.

College of Health and Life Sciences, Hamad Bin Khalifa University, Doha, Qatar.

KASH5 is the most recently identified member of the KASH domain family of tail anchored, outer nuclear membrane (ONM) and endoplasmic reticulum (ER) proteins. During meiosis prophase I, KASH5 and SUN1 form a complex that spans the nuclear envelope and which links the telomeres of meiotic chromosomes to cytoplasmic dynein. This connection is essential for homologous chromosome dynamics and pairing. Read More

View Article and Full-Text PDF

Effects of the surface charge of polyamidoamine dendrimers on cellular exocytosis and the exocytosis mechanism in multidrug-resistant breast cancer cells.

J Nanobiotechnology 2021 May 12;19(1):135. Epub 2021 May 12.

Institute of Pharmaceutics, College of Pharmaceutical Sciences, Zhejiang University, Room 409, Yuhangtang Road 866, 310058, Hangzhou, People's Republic of China.

Background: Polyamidoamine (PAMAM) dendrimer applications have extended from tumor cells to multidrug-resistant tumor cells. However, their transportation in multidrug-resistant tumor cells remains unclear. Herein, we investigated the exocytosis rule and mechanism of PAMAM dendrimers in multidrug-resistant tumor cells. Read More

View Article and Full-Text PDF

Crystalline ruthenium polypyridine nanoparticles: a targeted treatment of bacterial infection with multifunctional antibacterial, adhesion and surface-anchoring photosensitizer properties.

J Mater Chem B 2021 May;9(18):3808-3825

School of Life Sciences, Anhui Agricultural University, Hefei, 230036, China.

Photodynamic antibacterial therapy employs nanocomposites as an alternative to traditional antibiotics for the treatment of bacterial infections. However, many of these antibacterial materials are less effective towards bacteria than traditional drugs, either due to poor specificity or antibacterial activity. This can result in needless and excessive drug use in treatments. Read More

View Article and Full-Text PDF

N-aryl pyrido cyanine derivatives are nuclear and organelle DNA markers for two-photon and super-resolution imaging.

Nat Commun 2021 May 11;12(1):2650. Epub 2021 May 11.

Graduate School of Science, Nagoya University, Nagoya, Japan.

Live cell imaging using fluorescent DNA markers are an indispensable molecular tool in various biological and biomedical fields. It is a challenge to develop DNA probes that avoid UV light photo-excitation, have high specificity for DNA, are cell-permeable and are compatible with cutting-edge imaging techniques such as super-resolution microscopy. Herein, we present N-aryl pyrido cyanine (N-aryl-PC) derivatives as a class of long absorption DNA markers with absorption in the wide range of visible light. Read More

View Article and Full-Text PDF

A Ran-binding protein facilitates nuclear import of human papillomavirus type 16.

PLoS Pathog 2021 May 11;17(5):e1009580. Epub 2021 May 11.

Institute of Cellular Virology, Westphalian Wilhelms-University of Münster, Münster, Germany.

Human papillomaviruses (HPVs) utilize an atypical mode of nuclear import during cell entry. Residing in the Golgi apparatus until mitosis onset, a subviral complex composed of the minor capsid protein L2 and viral DNA (L2/vDNA) is imported into the nucleus after nuclear envelope breakdown by associating with mitotic chromatin. In this complex, L2 plays a crucial role in the interactions with cellular factors that enable delivery and ultimately tethering of the viral genome to mitotic chromatin. Read More

View Article and Full-Text PDF

Phenolic profile of jujube fruit subjected to gut microbiota fermentation and its antioxidant potential against ethyl carbamate-induced oxidative damage.

J Zhejiang Univ Sci B 2021 May;22(5):397-409

Department of Food Science and Nutrition, Zhejiang Key Laboratory for Agro-Food Processing, Zhejiang University, Hangzhou 310058, China.

Objectives: To evaluate the composition of bioactive substances and the antioxidant effects of jujube fruit under gut microbiota fermentation (GMF), and the inhibitory effect on cytotoxicity caused by ethyl carbamate (EC).

Methods: Changes in the contents of flavonoids, polyphenols, total sugars, and reducing sugars of jujube fruit after GMF (0, 2, 6, 12, 24, and 48 h) were determined. The oxidation resistance of fermented jujube fruits (from 0 to 48 h fermentation) was evaluated using in vitro 2,2'-azinobis-(3-ethylbenzthiazoline-6-sulphonate) (ABTS) and ferric reducing antioxidant power (FRAP) assays. Read More

View Article and Full-Text PDF

The Desensitized Channelrhodopsin-2 Photointermediate Contains 13-cis,15-syn Retinal Schiff Base.

Angew Chem Int Ed Engl 2021 May 11. Epub 2021 May 11.

Goethe-Universitat Frankfurt am Main, Biophysical Chemistry, GERMANY.

Channelrhodopsin-2 (ChR2) is a light-gated cation channel and was used to lay the foundations of optogenetics. Its dark state X-ray structure has been determined in 2017 for the wild-type, which is the prototype for all other ChR variants. However, the mechanistic understanding of the channel function is still incomplete in terms of structural changes after photon absorption by the retinal chromophore and in the framework of functional models. Read More

View Article and Full-Text PDF

Nuclear magnetic resonance studies on conformation and stability of mastoparan in methanol.

Authors:
Yoshinori Miura

J Pept Sci 2021 May 10:e3338. Epub 2021 May 10.

Center for Advanced Instrumental Analysis, Kyushu University, Kasuga, Japan.

Mastoparan is a small peptide composed of 14 amino acid residues found in wasp venom. It penetrates into cytoplasm through the cell membranes and then binds to a G protein to stimulate the release of histamine. Conformation and its thermal stability of mastoparan from Vespula lewisi (MP) in methanol are investigated by using proton nuclear magnetic resonance (NMR) spectroscopy. Read More

View Article and Full-Text PDF

Nuclear Lamin A/C Expression Is a Key Determinant of Paclitaxel Sensitivity.

Mol Cell Biol 2021 May 10. Epub 2021 May 10.

Sylvester Comprehensive Cancer Center, Department of Radiation Oncology, University of Miami Miller School of Medicine, Miami, FL 33136

Paclitaxel is a key member of the Taxane (Taxol/paclitaxel, docetaxel/taxotere) family of successful drugs used in the current treatment of several solid tumors, including ovarian cancer. The molecular target of Taxol/paclitaxel has been identified as tubulin, and paclitaxel binding alters the dynamics and thus stabilizes microtubule bundles. Traditionally, the anti-cancer mechanism of paclitaxel has been thought to originate from its interfering with the role of microtubules in mitosis, resulting in mitotic arrest and subsequent apoptosis. Read More

View Article and Full-Text PDF

Necrosis binding of Ac-Lys(IRDye800CW)-Tyr-octreotate: a consequence from cyanine-labeling of small molecules.

EJNMMI Res 2021 May 10;11(1):47. Epub 2021 May 10.

Department of Radiology and Nuclear Medicine/Molecular Genetics, Erasmus Medical Centre, 's-Gravendijkwal 230, 3015 CE, Rotterdam, The Netherlands.

Background: There is a growing body of nuclear contrast agents that are repurposed for fluorescence-guided surgery. New contrast agents are obtained by substituting the radioactive tag with, or adding a fluorescent cyanine to the molecular structure of antibodies or peptides. This enables intra-operative fluorescent detection of cancerous tissue, leading to more complete tumor resection. Read More

View Article and Full-Text PDF

Resveratrol Improves Mitochondrial Biogenesis Function and Activates PGC-1α Pathway in a Preclinical Model of Early Brain Injury Following Subarachnoid Hemorrhage.

Front Mol Biosci 2021 22;8:620683. Epub 2021 Apr 22.

Hainan Provincial Key Laboratory for Human Reproductive Medicine and Genetic Research, Department of Reproductive Medicine, The First Affiliated Hospital of Hainan Medical University, Haikou, China.

Peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α) has been shown to play a pivotal role in the regulation of mitochondrial biogenesis in diseases. Resveratrol (RSV), a natural polyphenolic reagent, has powerful antioxidant properties and the ability to scavenge mitochondrial reactive oxygen species (ROS) in a variety of central nervous system diseases. However, the underlying molecular mechanisms of RSV on mitochondrial biogenesis in early brain injury (EBI) following subarachnoid hemorrhage (SAH) remain poorly understood. Read More

View Article and Full-Text PDF

Oxidative Stress-Mediated YAP Dysregulation Contributes to the Pathogenesis of Pemphigus Vulgaris.

Front Immunol 2021 19;12:649502. Epub 2021 Apr 19.

Centre for Oral Immunobiology and Regenerative Medicine, Institute of Dentistry, Barts and The London School of Medicine and Dentistry, London, United Kingdom.

(PV) is a life-threatening autoimmune disease manifested with blisters in the skin and mucosa and caused by autoantibodies against adhesion protein desmoglein-3 (Dsg3) expressed in epithelial membrane linings of these tissues. Despite many studies, the pathogenesis of PV remains incompletely understood. Recently we have shown Dsg3 plays a role in regulating the yes-associated protein (YAP), a co-transcription factor and mechanical sensor, and constraining reactive oxygen species (ROS). Read More

View Article and Full-Text PDF

Transcriptome analysis of mild hypothermia protection against radiationinduced rat lung injury based on RNAseq.

Zhong Nan Da Xue Xue Bao Yi Xue Ban 2021 Apr;46(4):345-350

Drug Safety Evaluation Center of China Institute for Radiation Protection; Shanxi Key Laboratory of Drug Toxicology and Drug for Radiation Injury; Key Laboratory on Radiotoxicology and Radiopharmaceutical Preclinical Evaluation, China National Nuclear Corporation, Taiyuan 030006, China.

Objectives: To analyze the differentially expressed genes (DEGs) with radiation-induced rat lung injury, and to reveal the protective mechanism for mild hypothermia in the radiation-induced lung injury in rats at the transcriptome level.

Methods: A total of 10 male SD rats aged 6-8 weeks were randomly divided into 2 groups to establish a rat model of radiation-induced lung injury, and one group was treated with mild hypothermia. RNA was extracted from left lung tissue of each group, and sequenced by BGISEQ-500 platform. Read More

View Article and Full-Text PDF

Nuclear expression of NHERF1/EBP50 in Clear Cell Renal Cell Carcinoma.

Acta Histochem 2021 May 6;123(5):151717. Epub 2021 May 6.

Department of Radiobiology, National Atomic Energy Commission of Argentina (CNEA), Argentina; National Research Council of Argentina (CONICET), Av. General Paz 1499 (1650), San Martín, Buenos Aires, Argentina. Electronic address:

The Na/H exchange regulatory factor 1 or Ezrin-radixin-moesin-binding phosphoprotein 50 (NHERF1/EBP50) is an adaptor protein implicated in the stabilization of molecular complexes linking extracellular signals with the cytoskeleton machinery. NHERF1 expression at the cell cortex is associated with the maintenance of adherent junction integrity in polarized epithelia. The role of NHERF1 in cancer depends on its localization within the cell, acting, in most cases, as a tumor suppressor when localized at the cell membrane, and as an oncogene, when expressed in the cytoplasm or the nucleus of cancer cells. Read More

View Article and Full-Text PDF

Synthesis and preclinical evaluation of an AlF radiofluorinated bivalent PSMA ligand.

Eur J Med Chem 2021 May 1;221:113502. Epub 2021 May 1.

Department of Nuclear Medicine, Nanfang Hospital, Southern Medical University, 1838 Guangzhou North Road, Guangzhou, Guangdong Province, 510515, China. Electronic address:

Prostate-specific membrane antigen (PSMA) has become as an outstanding prostate cancer-related target for diagnostic imaging and targeted radiotherapy. Clinical studies on a few PSMA radiotracers are currently underway to determine their efficacy as imaging agents to detect prostate cancer. To improve tumor retention and tumor-to-normal tissue contrast, we herein report the synthesis and preclinical evaluation of an AlF-labeled bivalent PSMA ligand (F-Bi-PSMA). Read More

View Article and Full-Text PDF

Parental genome unification is highly error-prone in mammalian embryos.

Cell 2021 Apr 30. Epub 2021 Apr 30.

Max Planck Institute for Biophysical Chemistry, 37077 Göttingen, Germany. Electronic address:

Most human embryos are aneuploid. Aneuploidy frequently arises during the early mitotic divisions of the embryo, but its origin remains elusive. Human zygotes that cluster their nucleoli at the pronuclear interface are thought to be more likely to develop into healthy euploid embryos. Read More

View Article and Full-Text PDF

Inoculation with Bacillus amyloliquefaciens and mycorrhiza confers tolerance to drought stress and improve seed yield and quality of soybean plant.

Physiol Plant 2021 May 8. Epub 2021 May 8.

Department of Agricultural Microbiology, Faculty of Agriculture, Mansoura University, Mansoura, Egypt.

The present study aimed to evaluate the effect of Bacillus amyloliquefaciens and/or Arbuscular Mycorrhizal Fungi (AMF) as natural biofertilizers on biomass, yield and seed nutritive quality of soybean (Giza 111). The conditions investigated include a well-watered (WW) control and irrigation withholding at the seed development stage (R5, after 90 days from sowing) (DS). Co-inoculation with B. Read More

View Article and Full-Text PDF

Rapid and Low-cost Sampling for Detection of Airborne SARS-CoV-2 in Dehumidifier Condensate.

Biotechnol Bioeng 2021 May 8. Epub 2021 May 8.

Center for Advanced Sensor Technology, University of Maryland Baltimore County, Baltimore, MD, 21250, USA.

Airborne spread of COVID-19 by infectious aerosol is all but certain. However, easily implemented approaches to assess the actual environmental threat are currently unavailable. We present a simple approach with the potential to rapidly provide information about the prevalence of SARS-CoV-2 in the atmosphere at any location. Read More

View Article and Full-Text PDF

's Strategies and Effector Mechanisms for Host Cell Transformation: From Invasion to Immortalization.

Front Cell Dev Biol 2021 20;9:662805. Epub 2021 Apr 20.

Institute of Animal Pathology, Vetsuisse Faculty, University of Bern, Bern, Switzerland.

One of the first events that follows invasion of leukocytes by sporozoites is the destruction of the surrounding host cell membrane and the rapid association of the intracellular parasite with host microtubules. This is essential for the parasite to establish its niche within the cytoplasm of the invaded leukocyte and sets spp. apart from other members of the apicomplexan phylum such as and spp. Read More

View Article and Full-Text PDF

A Pro-Tumorigenic Effect of Heparanase 2 (Hpa2) in Thyroid Carcinoma Involves Its Localization to the Nuclear Membrane.

Front Oncol 2021 20;11:645524. Epub 2021 Apr 20.

Department of Otolaryngology, Head and Neck Surgery, Carmel Medical Center, Haifa, Israel.

Activity of the endo-beta-glucuronidase heparanase, capable of cleaving heparan sulfate (HS), is most often elevated in many types of tumors, associating with increased tumor metastasis and decreased patients' survival. Heparanase is therefore considered to be a valid drug target, and heparanase inhibitors are being evaluated clinically in cancer patients. Heparanase 2 (Hpa2) is a close homolog of heparanase that gained very little attention, likely because it lacks HS-degrading activity typical of heparanase. Read More

View Article and Full-Text PDF

Can 68Ga-PSMA PET/CT-derived prostate-specific membrane antigen expression parameters predict prostate-specific antigen response to enzalutamide treatment?

Nucl Med Commun 2021 May 6. Epub 2021 May 6.

Department of Nuclear Medicine Department of Medical Oncology, Health Science University Medical Faculty, Prof Dr Cemil Taşçioğlu City Hospital, Istanbul, Turkey.

Objective: In patients with metastatic castration-resistant prostate cancer (mCRPCa), enzalutamide is administered when docetaxel treatment fails. The purpose of the study was to evaluate the relationship between prostate-specific antigen (PSA) response and metabolic parameters obtained from 68Ga-PSMA PET/CT before treatment in this patient group.

Methods: From February 2018 to May 2020, 34 patients with mCRPCa were enrolled in this study. Read More

View Article and Full-Text PDF

A polymorphism in the promoter of FRAS1 is a candidate SNP associated with metastatic prostate cancer.

Prostate 2021 May 6. Epub 2021 May 6.

Department of Medical Oncology, Dana Farber Cancer Institute, Boston, Massachusetts, USA.

Background: Inflammation and one of its mediators, NF-kappa B (NFκB), have been implicated in prostate cancer carcinogenesis. We assessed whether germline polymorphisms associated with NFκB are associated with the risk of developing lethal disease (metastases or death from prostate cancer).

Methods: Using a Bayesian approach leveraging NFκB biology with integration of publicly available datasets we used a previously defined genome-wide functional association network specific to NFκB and lethal prostate cancer. Read More

View Article and Full-Text PDF

Mitochondrial Disruption Is Involved in the Effect of Nuclear Factor of Activated T cells, Cytoplasmic 4 on Aggravating Cardiomyocyte Hypertrophy.

J Cardiovasc Pharmacol 2021 May;77(5):557-569

Department of Pharmacy, School of Medicine, Guangxi University of Science and Technology, Liuzhou, Guangxi, People's Republic of China; and.

Abstract: Nuclear factor of activated T cells, cytoplasmic 4 (NFATc4), a nuclear transcription factor, has been implicated in cardiac hypertrophy through the enhancement of hypertrophic gene expression. However, the role of NFATc4 in mitochondrial modulation is mostly unknown. The current study aimed to investigate the role of NFATc4 in regulating mitochondrial function during phenylephrine (PE)-induced cardiac hypertrophy. Read More

View Article and Full-Text PDF

Mus musculus Barrier-To-Autointegration Factor 2 (Banf2) is Not Essential for Spermatogenesis or Fertility.

Cytogenet Genome Res 2021 May 5:1-11. Epub 2021 May 5.

Department of Histology and Embryology, School of Medicine, Yangzhou University, Yangzhou, China.

The barrier-to-autointegration factor (BAF) is widely expressed in most human tissues and plays a critical role in chromatin organization, nuclear envelope assembly, gonadal development, and embryonic stem cell self-renewal. Complete loss of BAF has been shown to lead to embryonic lethality and gonadal defects. The BAF paralog, namely, barrier-to-autointegration factor 2 (BANF2), exhibits a testis-predominant expression pattern in both humans and mice. Read More

View Article and Full-Text PDF

The LRR-TM protein PAN-1 interacts with MYRF to promote its nuclear translocation in synaptic remodeling.

Elife 2021 May 5;10. Epub 2021 May 5.

School of Life Science and Technology, ShanghaiTech University, Shanghai, China.

Neural circuits develop through a plastic phase orchestrated by genetic programs and environmental signals. We have identified a leucine-rich-repeat domain transmembrane protein PAN-1 as a factor required for synaptic rewiring in . PAN-1 localizes on cell membrane and binds with MYRF, a membrane-bound transcription factor indispensable for promoting synaptic rewiring. Read More

View Article and Full-Text PDF

Clinical parameters and nomograms for predicting lymph node metastasis detected with Ga-PSMA-PET/CT in prostate cancer patients candidate to definitive radiotherapy.

Prostate 2021 May 5. Epub 2021 May 5.

Department of Radiation Oncology, Faculty of Medicine, Hacettepe University, Ankara, Turkey.

Background: Defining the extent of disease spread with imaging modalities is crucial for therapeutic decision-making and definition of treatment. This study aimed to investigate whether clinical parameters and nomograms predict prostate-specific membrane antigen (PSMA)-positive lymph nodes in treatment-naïve nonmetastatic prostate cancer (PC) patients.

Materials And Methods: The clinical data of 443 PC patients (83. Read More

View Article and Full-Text PDF

GPER: A Novel Target To Treat Cardiovascular Disease In A Sex-Specific Manner?

Br J Pharmacol 2021 May 4. Epub 2021 May 4.

Department of Physiology, Anatomy and Microbiology and Centre for Cardiovascular Biology and Disease Research, School of Life Sciences, La Trobe University, Bundoora, Victoria, 3086, Australia.

As an agonist of the classical nuclear receptors, ERα and ERβ, estrogen has been understood to inhibit the development of cardiovascular disease in pre-menopausal women. Indeed, reduced levels of estrogen after menopause are believed to contribute to accelerated morbidity and mortality rates in women. However, estrogen replacement therapy has variable effects on cardiovascular risk in post-menopausal women that include increased serious adverse events. Read More

View Article and Full-Text PDF