653,312 results match your criteria nuclear protein


Patterns of T and B cell responses to Mycobacterium tuberculosis membrane-associated antigens and their relationship with disease activity in rheumatoid arthritis patients with latent tuberculosis infection.

PLoS One 2021 2;16(8):e0255639. Epub 2021 Aug 2.

Department of Clinical Immunology & Rheumatology, Sanjay Gandhi Postgraduate Institute of Medical Sciences (SGPGIMS), Lucknow, India.

This study was aimed at exploring whether latent tuberculosis infection (LTBI) contributes to the pathogenesis of immune-mediated inflammatory diseases in a TB endemic setting. We screened 198 rheumatoid arthritis (RA) patients with tuberculin skin test (TST) and studied 61 (median DAS28-ESR = 6.3) who were positive. Read More

View Article and Full-Text PDF

Circadian rhythm shows potential for mRNA efficiency and self-organized division of labor in multinucleate cells.

PLoS Comput Biol 2021 Aug 2;17(8):e1008828. Epub 2021 Aug 2.

Department of Mathematics, UCLA, Los Angeles, California, United States of America.

Multinucleate cells occur in every biosphere and across the kingdoms of life, including in the human body as muscle cells and bone-forming cells. Data from filamentous fungi suggest that, even when bathed in a common cytoplasm, nuclei are capable of autonomous behaviors, including division. How does this potential for autonomy affect the organization of cellular processes between nuclei? Here we analyze a simplified model of circadian rhythm, a form of cellular oscillator, in a mathematical model of the filamentous fungus Neurospora crassa. Read More

View Article and Full-Text PDF

Interactions between the Cell Membrane Repair Protein S100A10 and Phospholipid Monolayers and Bilayers.

Langmuir 2021 Aug 2. Epub 2021 Aug 2.

Department of Ophthalmology, Faculty of Medicine, Université Laval, Quebec City, QC, G1S 4L8 Canada.

Protein S100A10 participates in different cellular mechanisms and has different functions, especially at the membrane. Among those, it forms a ternary complex with annexin A2 and the C-terminal of AHNAK and then joins the dysferlin membrane repair complex. Together, they act as a platform enabling membrane repair. Read More

View Article and Full-Text PDF

Evaluation of the Higher Order Structure of Biotherapeutics Embedded in Hydrogels for Bioprinting and Drug Release.

Anal Chem 2021 Aug 2. Epub 2021 Aug 2.

Magnetic Resonance Center (CERM), University of Florence, and Consorzio Interuniversitario Risonanze Magnetiche di Metalloproteine (CIRMMP), Via L. Sacconi 6, Sesto Fiorentino 50019, Italy.

Biocompatible hydrogels for tissue regeneration/replacement and drug release with specific architectures can be obtained by three-dimensional bioprinting techniques. The preservation of the higher order structure of the proteins embedded in the hydrogels as drugs or modulators is critical for their biological activity. Solution nuclear magnetic resonance (NMR) experiments are currently used to investigate the higher order structure of biotherapeutics in comparability, similarity, and stability studies. Read More

View Article and Full-Text PDF

Slow-Onset, Potent Inhibition of Mandelate Racemase by 2-Formylphenylboronic Acid. An Unexpected Adduct Clasps the Catalytic Machinery.

Biochemistry 2021 08 2. Epub 2021 Aug 2.

Department of Biochemistry and Molecular Biology, Dalhousie University, Halifax, NS B3H 4R2, Canada.

-Carbonyl arylboronic acids such as 2-formylphenylboronic acid (2-FPBA) are employed in biocompatible conjugation reactions with the resulting iminoboronate adduct stabilized by an intramolecular N-B interaction. However, few studies have utilized these reagents as active site-directed enzyme inhibitors. We show that 2-FPBA is a potent reversible, slow-onset inhibitor of mandelate racemase (MR), an enzyme that has served as a valuable paradigm for understanding enzyme-catalyzed abstraction of an α-proton from a carbon acid substrate with a high p. Read More

View Article and Full-Text PDF

Purinergic signaling: a potential therapeutic target for depression and chronic pain.

Purinergic Signal 2021 Aug 2. Epub 2021 Aug 2.

Neuropharmacology Laboratory of Physiology Department, Basic Medical School, Nanchang University, Nanchang, Jiangxi, 330006, People's Republic of China.

The comorbid mechanism of depression and chronic pain has been a research hotspot in recent years. Until now, the role of purinergic signals in the comorbid mechanism of depression and chronic pain has not been fully understood. This review mainly summarizes the research results published in PubMed during the past 5 years and concludes that purinergic signaling is a potential therapeutic target for comorbid depression and chronic pain, and the purinergic receptors A1, A2A, P2X3, P2X4, and P2X7and P2Y, P2Y, and P2Y may be important factors. Read More

View Article and Full-Text PDF

Identification of ZmNF-YC2 and its regulatory network in maize flowering time.

J Exp Bot 2021 Aug 2. Epub 2021 Aug 2.

Synergetic Innovation Center of Henan Grain Crops and National Key, Laboratory of Wheat and Maize Crop Science, Henan Agricultural University, Longzihu University Park, Zhengdong New Area, Zhengzhou, Henan, China.

Flowering time is an important agronomic trait that determines the distribution and adaptation of plants. The accurate prediction of flowering time in elite germplasm is very critical for maize breeding. However, the molecular mechanisms underlying photoperiod response remain elusive in maize. Read More

View Article and Full-Text PDF

Nucleo-cytoplasmic shuttling of splicing factor SRSF1 is required for development and cilia function.

Elife 2021 Aug 2;10. Epub 2021 Aug 2.

Genome Regulation Section, MRC Human Genetics Unit, Institute of Genetics and Cancer, University of Edinburgh, Edinburgh, United Kingdom.

Shuttling RNA-binding proteins coordinate nuclear and cytoplasmic steps of gene expression. The SR family proteins regulate RNA splicing in the nucleus and a subset of them, including SRSF1, shuttles between the nucleus and cytoplasm affecting post-splicing processes. However, the physiological significance of this remains unclear. Read More

View Article and Full-Text PDF

Uniparental nuclear inheritance following bisexual mating in fungi.

Elife 2021 Aug 2;10. Epub 2021 Aug 2.

Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, United States.

Some remarkable animal species require an opposite-sex partner for their sexual development but discard the partner's genome before gamete formation, generating hemi-clonal progeny in a process called hybridogenesis. Here, we discovered a similar phenomenon, termed pseudosexual reproduction, in a basidiomycete human fungal pathogen, , where exclusive uniparental inheritance of nuclear genetic material was observed during bisexual reproduction. Analysis of strains expressing fluorescent reporter proteins revealed instances where only one of the parental nuclei was present in the terminal sporulating basidium. Read More

View Article and Full-Text PDF

Zika virus NS2A inhibits interferon signaling by degradation of STAT1 and STAT2.

Virulence 2021 Dec;12(1):1580-1596

Department of Life and Environmental Sciences, University of Cagliari, Monserrato, Italy.

The Interferon (IFN) response is crucial to restrain pathogenic infections. Investigations into flavivirus-host interactions reported that the high virulence is linked to innate immune evasion. Zika Virus (ZIKV) has developed diversified strategies to evade the innate immune system. Read More

View Article and Full-Text PDF
December 2021

CTRP13 Protects H9c2 Cells Against Hypoxia/Reoxygenation (H/R)-Induced Injury Via Regulating the AMPK/Nrf2/ARE Signaling Pathway.

Cell Transplant 2021 Jan-Dec;30:9636897211033275

Department of Cardiology, Kaifeng People's Hospital, Kaifeng 475000, China.

Myocardial infarction (MI) is identified as the myocardial necrosis due to myocardial ischemia/reperfusion (I/R) injury and remains a leading cause of mortality. C1q/TNF-related protein 13 (CTRP13) is a member of CTRP family that has been found to be involved in coronary artery disease (CAD). However, the role of CTRP13 in MI remains unclear. Read More

View Article and Full-Text PDF

Design, Optimization, and Structural Characterization of an Apoptosis-Inducing Factor Peptide Targeting Human Cyclophilin A to Inhibit Apoptosis Inducing Factor-Mediated Cell Death.

J Med Chem 2021 Aug 2. Epub 2021 Aug 2.

Institute of Biostructures and Bioimaging (IBB)-CNR, Via Mezzocannone, 16, 80134 Napoli, Italy.

Blocking the interaction between the apoptosis-inducing factor (AIF) and cyclophilin A (CypA) by the AIF fragment AIF(370-394) is protective against glutamate-induced neuronal cell death and brain injury in mice. Starting from AIF(370-394), we report the generation of the disulfide-bridged and shorter variant AIF(381-389) and its structural characterization by nuclear magnetic resonance (NMR) in the free and CypA-bound state. AIF(381-389) in both the free and bound states assumes a β-hairpin conformation similar to that of the fragment in the AIF protein and shows a highly reduced conformational flexibility. Read More

View Article and Full-Text PDF

Exploring chromatin structural roles of non-coding RNAs at imprinted domains.

Biochem Soc Trans 2021 Aug 2. Epub 2021 Aug 2.

Institute of Molecular Genetics of Montpellier (IGMM), Centre National de Recherche Scientifique (CNRS), Montpellier, France.

Different classes of non-coding RNA (ncRNA) influence the organization of chromatin. Imprinted gene domains constitute a paradigm for exploring functional long ncRNAs (lncRNAs). Almost all express an lncRNA in a parent-of-origin dependent manner. Read More

View Article and Full-Text PDF

Advances in chemical probing of protein -GlcNAc glycosylation: structural role and molecular mechanisms.

Chem Soc Rev 2021 Aug 2. Epub 2021 Aug 2.

Chemical Immunology Lab, Centre for Cooperative Research in Biosciences, CIC-bioGUNE, Basque Research and Technology Alliance (BRTA), Derio 48160, Biscay, Spain. and Ikerbasque, Basque Foundation for Science, Bilbao 48013, Spain.

The addition of O-linked-β-d-N-acetylglucosamine (O-GlcNAc) onto serine and threonine residues of nuclear and cytoplasmic proteins is an abundant, unique post-translational modification governing important biological processes. O-GlcNAc dysregulation underlies several metabolic disorders leading to human diseases, including cancer, neurodegeneration and diabetes. This review provides an extensive summary of the recent progress in probing O-GlcNAcylation using mainly chemical methods, with a special focus on discussing mechanistic insights and the structural role of O-GlcNAc at the molecular level. Read More

View Article and Full-Text PDF

Blastocyst trophectoderm endocytic activation, a marker of adverse developmental programming.

Reproduction 2021 Aug 1. Epub 2021 Aug 1.

T Fleming, Biological Sciences, University of Southampton, Southampton, United Kingdom of Great Britain and Northern Ireland.

The mouse preimplantation embryo is sensitive to its environment including maternal dietary protein restriction which can alter the developmental programme and affect lifetime health. Previously, we have shown maternal low protein diet (LPD) causes reduction in blastocyst mTORC1 signalling coinciding with reduced availability of branched-chain amino acids (BCAAs) in surrounding uterine fluid. BCAA deficiency leads to increased endocytosis and lysosome biogenesis in blastocyst trophectoderm (TE), a response to promote compensatory histotrophic nutrition. Read More

View Article and Full-Text PDF

Role of tight junction-associated MARVEL protein marvelD3 in migration and epithelial-mesenchymal transition of hepatocellular carcinoma.

Cell Adh Migr 2021 Dec;15(1):249-260

Experimental Center, Beijing Friendship Hospital, Capital Medical University, Beijing, China.

MarvelD3, a recently identified tight junction membrane protein, could be associated with hepatocellular carcinoma (HCC). We aimed to investigate the role of marvelD3 in Epithelial-Mesenchymal Transition (EMT) and migration of HCC and explore the underlying molecular mechanisms. First, we assessed marvlD3 expression in HCC and normal liver tissues and found loss of marvelD3 expression was significantly correlated with the occurrence and TNM stage of HCC. Read More

View Article and Full-Text PDF
December 2021

Exendin-4 stimulates autophagy in pancreatic β-cells via the RAPGEF/EPAC-Ca-PPP3/calcineurin-TFEB axis.

Autophagy 2021 Aug 2:1-17. Epub 2021 Aug 2.

Biosciences Institute, Newcastle University, Newcastle Upon Tyne, UK.

Macroautophagy/autophagy is critical for the regulation of pancreatic β-cell mass and its deregulation has been implicated in the pathogenesis of type 2 diabetes (T2D). We have previously shown that treatment of pancreatic β-cells with the GLP1R (glucagon like peptide 1 receptor) agonist exendin-4 stimulates autophagic flux in a setting of chronic nutrient excess. The aim of this study was to identify the underlying pathways contributing to enhanced autophagic flux. Read More

View Article and Full-Text PDF

Multiple structural rearrangements mediated by high-plasticity regions in Atg3 are key for efficient conjugation of Atg8 to PE during autophagy.

Autophagy 2021 Jul 31:1-4. Epub 2021 Jul 31.

Life Sciences Institute, University of Michigan, Ann Arbor, MI, USA.

The Atg3 protein is highly homologous from yeast to human. Atg3 functions as an E2-like enzyme promoting conjugation of Atg8-family proteins to phosphatidylethanolamine (PE), a lipid molecule embedded in the growing phagophore membrane during stress-induced autophagy. Over the last decade, Atg3 became one of the most explored autophagy proteins, resulting in observations that provided specific insights into the structural mechanisms of its function. Read More

View Article and Full-Text PDF

Quercetin Inhibits the Epithelial to Mesenchymal Transition through Suppressing Akt Mediated Nuclear Translocation of β-Catenin in Lung Cancer Cell Line.

Nutr Cancer 2021 Aug 2:1-13. Epub 2021 Aug 2.

Department of Pharmacology, The Blue Lab, Molecular Medicine and Toxicology Division, Saveetha Dental College, Saveetha Institute of Medical and Technical Sciences (SIMATS), Chennai, India.

Lung cancer is a first leading cause of cancer related death worldwide. Quercetin (QUE) has chemo-preventive effect against a variety of cancers. However, the molecular mechanism of QUE mediated inhibition of cancer cell migration and epithelial to mesenchymal transition (EMT) is not clear in lung cancer. Read More

View Article and Full-Text PDF

Fab Fragment of Immunoglobulin Y Modulates NF-κB and MAPK Signaling through TLR4 and αVβ3 Integrin and Inhibits the Inflammatory Effect on R264.7 Macrophages.

J Agric Food Chem 2021 Aug 2. Epub 2021 Aug 2.

Key Laboratory of Environment Correlative Dietology, National Research and Development Centre for Egg Processing, College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.

High-purity Fab fragment and immunoglobulin Y (IgY) were prepared to evaluate their anti-inflammatory activity in the lipopolysaccharide (LPS)-induced Raw 264.7 macrophage system. Compared with IgY, the Fab fragment possessed a greater potency in inhibiting the inflammation by nitric oxide (NO)/inducible nitric oxide synthase (iNOS) and prostaglandin-E2 (PGE2)/cyclooxygenase-2 (COX-2) pathways. Read More

View Article and Full-Text PDF

Piwi-piRNA complexes induce stepwise changes in nuclear architecture at target loci.

EMBO J 2021 Aug 2:e108345. Epub 2021 Aug 2.

Department of Molecular Biology, Keio University School of Medicine, Tokyo, Japan.

PIWI-interacting RNAs (piRNAs) are germline-specific small RNAs that form effector complexes with PIWI proteins (Piwi-piRNA complexes) and play critical roles for preserving genomic integrity by repressing transposable elements (TEs). Drosophila Piwi transcriptionally silences specific targets through heterochromatin formation and increases histone H3K9 methylation (H3K9me3) and histone H1 deposition at these loci, with nuclear RNA export factor variant Nxf2 serving as a co-factor. Using ChEP and DamID-seq, we now uncover a Piwi/Nxf2-dependent target association with nuclear lamins. Read More

View Article and Full-Text PDF

Progressive use of multispectral imaging flow cytometry in various research areas.

Analyst 2021 Aug 2. Epub 2021 Aug 2.

University of Maribor, Faculty of Medicine, Institute of Biomedical Sciences, Taborska ulica 8, SI-2000 Maribor, Slovenia. and University of Maribor, Faculty of Medicine, Department of Pharmacology, Taborska ulica 8, SI-2000 Maribor, Slovenia.

Multi-spectral imaging flow cytometry (MIFC) has become one of the most powerful technologies for investigating general analytics, molecular and cell biology, biotechnology, medicine, and related fields. It combines the capabilities of the morphometric and photometric analysis of single cells and micrometer-sized particles in flux with regard to thousands of events. It has become the tool of choice for a wide range of research and clinical applications. Read More

View Article and Full-Text PDF

Single-lesion Prostate-specific Membrane Antigen Protein Expression (PSMA) and Response to [Lu]-PSMA-ligand Therapy in Patients with Castration-resistant Prostate Cancer.

Eur Urol Open Sci 2021 Aug 30;30:63-66. Epub 2021 Jun 30.

Department of Urology, Medical University of Vienna, Vienna, Austria.

Initial reports of a clinical response in patients treated with the radioligand [Lu]-PSMA-617 for castration-resistant prostate cancer (CRPC) are promising, despite known inter- and intrapatient heterogeneity. In metastatic CRPC, we examined the association of baseline immunohistochemical (IHC) expression of prostate-specific membrane antigen (PSMA) in a single lesion and responsiveness to [Lu]-PSMA-617 therapy, measured as the PSMA maximum standardized uptake value (SUV). Between 2015 and 2020, 19 patients with multiple metastases underwent single-lesion biopsy, [Ga]-PSMA positron emission tomography (PET) imaging, and treatment with [Lu]-PSMA-617. Read More

View Article and Full-Text PDF

Management of Thyrotoxicosis Induced by PD1 or PD-L1 Blockade.

J Endocr Soc 2021 Sep 15;5(9):bvab093. Epub 2021 May 15.

Endocrinology Unit, Azienda Ospedaliero-Universitaria Pisana and Department of Clinical and Experimental Medicine, University Hospital of Pisa, Pisa 56124, Italy.

Context: Thyrotoxicosis is a common immune-related adverse event in patients treated with programmed cell death protein-1 (PD1) or programmed cell death protein ligand-1 (PD-L1) blockade. A detailed endocrinological assessment, including thyroid ultrasound and scintigraphy, is lacking, as are data on response to treatment and follow-up.

Objective: The aim of this study was to better characterize the thyrotoxicosis secondary to immune checkpoint inhibitors, gaining insights into pathogenesis and treatment. Read More

View Article and Full-Text PDF
September 2021

Amyloid and Tau Induce Cell Death Independently of TSPO Polymerization and Density Changes.

ACS Omega 2021 Jul 14;6(29):18719-18727. Epub 2021 Jul 14.

Department of Psychiatry, University Hospitals of Geneva, 1205 Genève, Switzerland.

Apoptosis-dependent cell death of astrocytes has been described in Alzheimer's disease and is linked to the presence of two markers of the pathology: the β-amyloid peptide (Aβ) and the hyperphosphorylated Tau protein. Astrocytes also show reactive states characterized by the overexpression of the 18 kDa translocator protein (TSPO). However, TSPO is also known, in other areas of research, to participate in cell proliferation and death. Read More

View Article and Full-Text PDF

Metabolomics of Artichoke Bud Extract in Spontaneously Hypertensive Rats.

ACS Omega 2021 Jul 12;6(29):18610-18622. Epub 2021 Jul 12.

Department of Clinical Pharmacology, Hunan Key Laboratory of Pharmacogenetics, and National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha 410008, P. R. China.

Hypertension adversely affects the quality of life in humans across modern society. Studies have attributed increased reactive oxygen species production to the pathophysiology of hypertension. So far, a specific drug to control the disease perfectly has not been developed. Read More

View Article and Full-Text PDF

prediction of secretory proteins of and that target the host cell nucleus.

Heliyon 2021 Jul 5;7(7):e07204. Epub 2021 Jun 5.

Department of Medicine (Division of Infectious Diseases), Department of Microbiology and Immunology, State University of New York at Stony Brook, NY, Stony Brook, USA.

Liver flukes , and are causing agents of liver and hepatobiliary diseases. A remarkable difference between such worms is the fact that and are carcinogenic organisms whereas is not carcinogenic. The release of secretory factors by carcinogenic flukes seems to contribute to cancer development however if some of these target the host cell nuclei is unknown. Read More

View Article and Full-Text PDF

Association of the Variant rs6984094, Which Lengthens Telomeres, with Systemic Lupus Erythematosus Susceptibility in Chinese Populations.

J Immunol Res 2021 13;2021:7079359. Epub 2021 Jul 13.

Nephrology Hospital, The First Affiliated Hospital of Zhengzhou University, Zhengzhou University, Henan 4500052, China.

A recent genome-wide association study (GWAS) of Asian ancestry reported that single nucleotide polymorphism (SNP) in (telomerase reverse transcriptase) was associated with systemic lupus erythematosus (SLE). TERT has a critical role in maintaining the chromosomal stability and the length of telomere. Given that only a small portion of the genetic heritability of SLE has been explained so far, we aimed to identify novel loci in telomere-related genes responsible for SLE susceptibility in Chinese populations. Read More

View Article and Full-Text PDF

High Migration and Invasion Ability of PGCCs and Their Daughter Cells Associated With the Nuclear Localization of S100A10 Modified by SUMOylation.

Front Cell Dev Biol 2021 16;9:696871. Epub 2021 Jul 16.

Department of Pathology, Tianjin Union Medical Center, Nankai University, Tianjin, China.

Our previous studies have confirmed that cobalt chloride (CoCl) or chemoradiotherapy could induce the formation of polyploid tumor giant cells (PGCCs). Polyploid giant cancer cells are a special subpopulation of cancer cells that contribute to solid tumor heterogeneity. The size of PGCC was at least three times larger than regular diploid cancer cells. Read More

View Article and Full-Text PDF

Knockout Gene-Based Evidence for PIWI-Interacting RNA Pathway in Mammals.

Front Cell Dev Biol 2021 14;9:681188. Epub 2021 Jul 14.

State Key Laboratory of Reproductive Medicine, Department of Histology and Embryology, School of Basic Medical Sciences, Nanjing Medical University, Nanjing, China.

The PIWI-interacting RNA (piRNA) pathway mainly consists of evolutionarily conserved protein factors. Intriguingly, many mutations of piRNA pathway factors lead to meiotic arrest during spermatogenesis. The majority of piRNA factor-knockout animals show arrested meiosis in spermatogenesis, and only a few show post-meiosis male germ cell arrest. Read More

View Article and Full-Text PDF