729,279 results match your criteria nuclear

Solution structure of the Myb domain of Terfa derived from Zebrafish interacting with both human and plant telomeric DNA.

Biochem Biophys Res Commun 2021 May 10;559:252-258. Epub 2021 May 10.

PCG-Biotech, Ltd., Yonsei Engineering Research Park, Yonsei University, Seoul, 120-749, Republic of Korea; Department of Biochemistry, College of Life Science and Biotechnology, Yonsei University, Seoul, 120-749, Republic of Korea. Electronic address:

Telomeric repeat binding factor a (Terfa) derived from zebrafish is a homologous protein with human telomeric repeat binding factor 2 (TRF2). Terfa is known as a senescence-associated biomarker in various research through the zebrafish animal model. In addition, according to the findings so far, it has been confirmed that human or plant telomere binding proteins bind to telomeric DNA specialized for each species, but, in our result, Terfa shows it strongly binds to both human or plant type telomeric DNA. Read More

View Article and Full-Text PDF

Time series analysis of the in-hospital diagnostic process in suspected pulmonary embolism evaluated by computed tomography: An explorative study.

Eur J Radiol 2021 May 8;140:109758. Epub 2021 May 8.

Department of Diagnostic and Interventional Radiology and Nuclear Medicine, University Medical Center Hamburg-Eppendorf, Hamburg, Germany. Electronic address:

Purpose: This retrospective study aims to analyze the distribution of demand and the duration of the diagnostic workup of suspected pulmonary embolism (PE) using computed tomography pulmonary angiography (CTPA).

Methods: Time data from physical examination to report creation were identified for each CTPA in 2013 and 2018 at a tertiary hospital. Multivariable multinomial logistic and linear regression models were used to evaluate differences between 3 time intervals (I1: 6am-2pm, I2: 2pm-10pm, I3: 10pm-6am). Read More

View Article and Full-Text PDF

Robust hetero-MoO/MoO@N-doped carbon nanobelts decorated with oxygen deficiencies as high-performance anodes for potassium/sodium storage.

J Colloid Interface Sci 2021 Apr 24;599:730-740. Epub 2021 Apr 24.

School of Chemical and Biomolecular Engineering, The University of Sydney, Australia. Electronic address:

Hetero-MoO/MoO@N-doped carbon nanobelt anodes (h-MoO/MoO@NC) with long lifespan and superior rate capability were proposed by a simple in situ reduction tactic, in which pristine MoO was transformed into heterogeneous MoO/MoO. The hetero-MoO/MoO architecture significantly improves the electronic conductivity and affords abundant oxygen deficiencies. Meanwhile, the synergistic effect of internal MoO/MoO heterostructure and outer N-doped carbon layer (NC) accomplishes a balance of sustainable potassium/sodium storage and ultra-durable structure stability. Read More

View Article and Full-Text PDF

Comparison of brain F-18 FDG PET/MRI with PET/CT imaging in pediatric patients.

Clin Neurol Neurosurg 2021 Apr 27;206:106669. Epub 2021 Apr 27.

Istanbul University-Cerrahpasa, School of Medicine, Department of Nuclear Medicine, Istanbul, Turkey.

Background: Standardized uptake values (SUVs) are important indexes for evaluating the accuracy of disease diagnoses achieved via fluoro-18 deoxyglucose (F-18 FDG) positron emission tomography/computed tomography (PET/CT) and positron emission tomography/magnetic resonance imaging (PET/MRI). The purpose of this study is to describe normal cerebral FDG uptake in the pediatric population and compare SUVmax/mean results for brain images obtained from PET/CT and PET/MRI in neurologically healthy pediatric examinees.

Methods: This study included 20 patients who were < 18 years of age and were without intracranial malignancy and/or brain disorders. Read More

View Article and Full-Text PDF

Synthesis and biological evaluation of novel F-labeled phenylbenzofuran-2-carboxamide derivative for detection of orexin 1 receptor in the brain.

Bioorg Med Chem Lett 2021 May 10:128098. Epub 2021 May 10.

Department of Patho-Functional Bioanalysis, Graduate School of Pharmaceutical Sciences, Kyoto University, 46-29 Yoshida Shimoadachi-cho, Sakyo-ku, Kyoto 606-8501, Japan. Electronic address:

Although the orexin 1 receptor (OXR) in the brain is considered to regulate reward and feeding, the in vivo function of OXR has not been fully elucidated. In vivo imaging of OXR with positron emission tomography (PET) may be useful to further understand the molecular details of OXR. In this study, we newly designed and synthesized a phenylbenzofuran-2-carboxamide (PBC) derivative ([F]PBC-1) and evaluated its utility as a PET probe targeting OXR in the brain. Read More

View Article and Full-Text PDF

Evolution of protective symbiosis in palaemonid shrimps (Decapoda: Caridea) with emphases on host spectrum and morphological adaptations.

Mol Phylogenet Evol 2021 May 10:107201. Epub 2021 May 10.

Simon F. S. Li Marine Science Laboratory, School of Life Sciences, The Chinese University of Hong Kong, Shatin, Hong Kong, China; Oxford University Museum of Natural History, Parks Road, Oxford OX1 3PW, UK. Electronic address:

Palaemonidae is the most speciose caridean shrimp family, with its huge biodiversity partially generated via symbiosis with various marine invertebrates. Previous studies have provided insights into the evolution of protective symbiosis in this family with evidence for frequent inter-phyla host switches, but the comprehensiveness of evolutionary pathways is hampered by the resolution of the previous phylogenetic trees as well as the taxon coverage. Furthermore, several critical issues related to the evolution of a symbiotic lifestyle, including the change in host spectrum and corresponding morphological adaptations, remain largely unresolved. Read More

View Article and Full-Text PDF

Biogeographic origins of southern African Silene (Caryophyllaceae).

Mol Phylogenet Evol 2021 May 10:107199. Epub 2021 May 10.

Department of Biological and Environmental Sciences, University of Gothenburg, Carl Skottsbergs Gata 22 B, 413 19 Gothenburg, Sweden; Gothenburg Global Biodiversity Centre, Box 461, 405 30 Gothenburg, Sweden.

Silene (Caryophyllaceae) is distributed predominantly in the northern Hemisphere, where it is most diverse around the Mediterranean Basin. The genus is also well represented in North Africa, extending into tropical, sub-Saharan and southern Africa. Eight native species are recognized in southern Africa, taxonomically placed in two sections: Elisanthe and Silene s. Read More

View Article and Full-Text PDF

Speckled 100 kDa gene in pigs: Alternative splicing, subcellular localization, and response to interferon-α stimulation.

Gene 2021 May 10:145710. Epub 2021 May 10.

Institute of Animal Husbandry, Heilongjiang Academy of Agricultural Sciences, Harbin, 150086, China.

Speckled 100 kDa (Sp100) plays an important role in the antiviral immune response, however, little is known about porcine Sp100. In this study, porcine Sp100 was cloned and its response to interferon (IFN) α was identified. We obtained the cDNA (V1) of the gene, SP100, and seven alternative splicing variants (V2-8). Read More

View Article and Full-Text PDF

Degradation of p53 by HPV16-E6 variants isolated from cervical cancer specimens of Moroccan women.

Gene 2021 May 10:145709. Epub 2021 May 10.

Biology and Medical Research Unit, National Centre for Energy, Nuclear Sciences and Techniques (CNESTEN), Rabat, Morocco. Electronic address:

Cervical cancer is the second most diagnosed cancer in Moroccan women. The main etiological factor for developing cervical cancer is the persistent infection with HPV16. Genetic studies have reported the occurrence of amino acid variations within the E6 oncoprotein that promotes host cell transformation by targeting p53 for degradation. Read More

View Article and Full-Text PDF

Diphlorethohydroxycamalol isolated from Ishige okamurae prevents HO-induced oxidative damage via BMP2/Runx2 signaling in osteoblastic MC3T3-E1 cells.

Fitoterapia 2021 May 10:104921. Epub 2021 May 10.

Department of Food and Nutrition, College of Medical and Life Science, Silla University, Busan 46958, Republic of Korea. Electronic address:

Accumulating evidence has shown an association between osteoporosis and oxidative damage. In the present study, the protective effects of diphlorethohydroxycarmalol (DPHC) isolated from the brown algae Ishige okamurae against HO-induced oxidative damage via BMP2/ runt-related transcription factor 2 (Runx2) signaling were investigated using MC3T3-E1 osteoblastic cells. DPHC counteracted the reduction in cell viability caused by HO exposure and protected against HO-induced dysfunction, demonstrated by improved cellular alkaline phosphatase (ALP) activity and calcium deposition. Read More

View Article and Full-Text PDF

Pronounced anti-neuroinflammatory jasmonates and terpenes isolated from lychee seeds.

Fitoterapia 2021 May 10:104924. Epub 2021 May 10.

School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, China; Guangdong Engineering Research Center for Lead Compounds & Drug Discovery, Guangzhou 510006, China. Electronic address:

Lychee is a favorite fruit of the Cantonese and native to Southeast Asia. In this study, the anti-neuroinflammatory bioactive compounds of lychee seeds have been carried out. Five new jasmonates (1, 2, 6-8) and seventeen known compounds were isolated using a series of chemical and chromatographic methods. Read More

View Article and Full-Text PDF

Deciphering the glioblastoma phenotype by Computed Tomography radiomics.

Radiother Oncol 2021 May 10. Epub 2021 May 10.

Department of Radiation Oncology (Maastro), GROW School for Oncology, Maastricht University Medical Centre+, Maastricht, The Netherlands.

Introduction: Glioblastoma (GBM) is the most common malignant primary brain tumour which has, despite extensive treatment, a median overall survival of 15 months. Radiomics is the high-throughput extraction of large amounts of image features from radiographic images, which allows capturing the tumour phenotype in 3D and in a non-invasive way. In this study we assess the prognostic value of CT radiomics for overall survival in patients with a GBM. Read More

View Article and Full-Text PDF

Fenofibrate, a PPARα agonist, reduces hepatic fat accumulation through the upregulation of TFEB-mediated lipophagy.

Metabolism 2021 May 10:154798. Epub 2021 May 10.

Department of Endocrinology and Metabolism, Kyung Hee University Hospital at Gangdong, Seoul, Republic of Korea; Division of Endocrinology and Metabolism, Department of Internal Medicine, Kyung Hee University School of Medicine, Seoul, Republic of Korea. Electronic address:

Background: Recent studies have shown that dysregulation of autophagy is involved in the development of nonalcoholic fatty liver disease (NAFLD). Transcription factors E3 (TFE3) and EB (TFEB) are master regulators of the transcriptional response of basic cellular processes such as lysosomal biogenesis and autophagy. Here, we investigated the role of fenofibrate, a PPARα agonist, in promotion of intracellular lipid clearance by upregulation of TFEB/TFE3. Read More

View Article and Full-Text PDF

Unlocking the PIP-box: A peptide library reveals interactions that drive high affinity binding to human PCNA.

J Biol Chem 2021 May 10:100773. Epub 2021 May 10.

Institute of Photonics and Advanced Sensing, School of Biological Sciences, The University of Adelaide, Adelaide, South Australia 5005, Australia. Electronic address:

The human sliding clamp Proliferating Cell Nuclear Antigen (hPCNA) interacts with over 200 proteins through a conserved binding motif, the PIP-box, to orchestrate DNA replication and repair. It is not clear how changes to the features of a PIP-box modulate protein binding and thus how they fine tune downstream processes. Here we present a systematic study of each position within the PIP-box to reveal how hPCNA-interacting peptides bind with drastically varied affinities. Read More

View Article and Full-Text PDF

Decoding the organization, dynamics, and function of the 4D genome.

Dev Cell 2021 May 7. Epub 2021 May 7.

Helmholtz Pioneer Campus, Helmholtz Zentrum München, 85764 Neuherberg, Germany; Biomedical Center (BMC), Faculty of Medicine, LMU Munich, Germany. Electronic address:

Understanding how complex cell-fate decisions emerge at the molecular level is a key challenge in developmental biology. Despite remarkable progress in decoding the contribution of the linear epigenome, how spatial genome architecture functionally informs changes in gene expression remains unclear. In this review, we discuss recent insights in elucidating the molecular landscape of genome folding, emphasizing the multilayered nature of the 3D genome, its importance for gene regulation, and its spatiotemporal dynamics. Read More

View Article and Full-Text PDF

Silencing Hoxa2 reverses dexamethasone-induced dysfunction of MC3T3-E1 osteoblasts and osteoporosis in rats.

Adv Clin Exp Med 2021 May 13. Epub 2021 May 13.

Department of Orthopedics, Liuzhou People's Hospital, Guangxi Medical University, China.

Background: Osteoporosis is damaging the health of women worldwide. Osteoporosis results from the imbalance between bone resorption and formation, which may be regulated by homeobox A2 (Hoxa2). However, the specific role and mechanism of Hoxa2 in osteogenesis and dexamethasone (Dex)-induced osteoporosis remain unknown. Read More

View Article and Full-Text PDF

Harmonizing neuropsychological assessment for mild neurocognitive disorders in Europe.

Alzheimers Dement 2021 May 13. Epub 2021 May 13.

Department of Medicine and Surgery, Institute of Gerontology and Geriatrics, University of Perugia, Perugia, Italy.

Introduction: Harmonized neuropsychological assessment for neurocognitive disorders, an international priority for valid and reliable diagnostic procedures, has been achieved only in specific countries or research contexts.

Methods: To harmonize the assessment of mild cognitive impairment in Europe, a workshop (Geneva, May 2018) convened stakeholders, methodologists, academic, and non-academic clinicians and experts from European, US, and Australian harmonization initiatives.

Results: With formal presentations and thematic working-groups we defined a standard battery consistent with the U. Read More

View Article and Full-Text PDF

Reactor-based boron neutron capture therapy for 44 cases of recurrent and refractory high-grade meningiomas with long-term follow-up.

Neuro Oncol 2021 May 13. Epub 2021 May 13.

Department of Neurosurgery, Osaka Medical College, Takatsuki, Osaka, Japan.

Background: High-grade meningioma (HGM) is difficult to treat, and recurrent HGM after radiotherapy has an especially poor prognosis. We retrospectively analyzed the cases of 44 consecutive patients with recurrent and refractory HGM who were treated by reactor-based boron neutron capture therapy (BNCT).

Methods: In 2005-2019, we treated 44 recurrent and refractory HGMs by reactor-based BNCT. Read More

View Article and Full-Text PDF

Risk perception of the pre-distribution of stable iodine to guardians of children living around the Genkai Nuclear Power Plant, Saga Prefecture, Japan.

PLoS One 2021 13;16(5):e0250570. Epub 2021 May 13.

Department of Global Health, Medicine and Welfare, Atomic Bomb Disease Institute, Nagasaki University, Nagasaki, Japan.

Iodine thyroid blocking (ITB) is effective for preventing childhood thyroid cancer when radioactive iodine is released into the environment during a nuclear power plant accident. Japan employs the pre-distribution of stable iodine (PDSI) to residents living near nuclear power plants; however, the number of residents who have actually received stable iodine to date remains limited. The aim of this study was to evaluate the profile of guardians of children living around the Genkai Nuclear Power Plant (GNPP) in Japan. Read More

View Article and Full-Text PDF

RNA editing at a limited number of sites is sufficient to prevent MDA5 activation in the mouse brain.

PLoS Genet 2021 May 13;17(5):e1009516. Epub 2021 May 13.

Department of RNA Biology and Neuroscience, Graduate School of Medicine, Osaka University, Suita, Japan.

Adenosine deaminase acting on RNA 1 (ADAR1), an enzyme responsible for adenosine-to-inosine RNA editing, is composed of two isoforms: nuclear p110 and cytoplasmic p150. Deletion of Adar1 or Adar1 p150 genes in mice results in embryonic lethality with overexpression of interferon-stimulating genes (ISGs), caused by the aberrant recognition of unedited endogenous transcripts by melanoma differentiation-associated protein 5 (MDA5). However, among numerous RNA editing sites, how many RNA sites require editing, especially by ADAR1 p150, to avoid MDA5 activation and whether ADAR1 p110 contributes to this function remains elusive. Read More

View Article and Full-Text PDF

Pathophysiology and Therapeutic Approaches to Cardiac Amyloidosis.

Circ Res 2021 May 13;128(10):1554-1575. Epub 2021 May 13.

Division of Cardiology, Cardiovascular Research Laboratory for the Elderly, Columbia University Irving Medical Center, NY.

Often considered a rare disease, cardiac amyloidosis is increasingly recognized by practicing clinicians. The increased rate of diagnosis is in part due the aging of the population and increasing incidence and prevalence of cardiac amyloidosis with advancing age, as well as the advent of noninvasive methods using nuclear scintigraphy to diagnose transthyretin cardiac amyloidosis due to either variant or wild type transthyretin without a biopsy. Perhaps the most important driver of the increased awareness is the elucidation of the biologic mechanisms underlying the pathogenesis of cardiac amyloidosis which have led to the development of several effective therapies with differing mechanisms of actions. Read More

View Article and Full-Text PDF

Selective Nonmethylated CpG DNA Recognition Mechanism of Cysteine Clamp Domains.

J Am Chem Soc 2021 May 13. Epub 2021 May 13.

Beijing Nuclear Magnetic Resonance Center, College of Chemistry and Molecular Engineering, and School of Life Sciences, Peking University, Beijing 100871, China.

Methylation of DNA at CpG sites is a major mark for epigenetic regulation, but how transcription factors are influenced by CpG methylation is not well understood. Here, we report the molecular mechanisms of how the TCF (T-cell factor) and GEF (glucose transporter 4 enhancer factor) families of proteins selectively target unmethylated DNA sequences with a C-clamp type zinc finger domain. The structure of the C-clamp domain from human GEF family protein HDBP1 (C-clamp) in complex with DNA was determined using NMR spectroscopy, which adopts a unique zinc finger fold and selectively binds RCCGG (R = A/G) DNA sequences with an "Arg···Trp-Lys-Lys" DNA recognition motif inserted in the major groove. Read More

View Article and Full-Text PDF

Inclusion of the C-Terminal Domain in the β-Sheet Core of Heparin-Fibrillized Three-Repeat Tau Protein Revealed by Solid-State Nuclear Magnetic Resonance Spectroscopy.

J Am Chem Soc 2021 May 13. Epub 2021 May 13.

Department of Chemistry, Massachusetts Institute of Technology, 170 Albany Street, Cambridge, Massachusetts 02139, United States.

Many neurodegenerative diseases such as Alzheimer's disease are characterized by pathological β-sheet filaments of the tau protein, which spread in a prion-like manner in patient brains. To date, high-resolution structures of tau filaments obtained from patient brains show that the β-sheet core only includes portions of the microtubule-binding repeat domains and excludes the C-terminal residues, indicating that the C-terminus is dynamically disordered. Here, we use solid-state NMR spectroscopy to identify the β-sheet core of full-length 0N3R tau fibrillized using heparin. Read More

View Article and Full-Text PDF

Methamphetamine Enhances HIV-Induced Aberrant Proliferation of Neural Progenitor Cells via the FOXO3-Mediated Mechanism.

Mol Neurobiol 2021 May 13. Epub 2021 May 13.

Department of Biochemistry and Molecular Biology, University of Miami Miller School of Medicine, 1011 NW 15th Street, Miami, FL, 33136, USA.

Maintaining an intact pool of neural progenitor cells (NPCs) is crucial for generating new and functionally active neurons. Methamphetamine (METH) can exacerbate the HIV-induced deficit of adult neurogenesis; however, potential mechanisms of this influence are still poorly understood. In the present study, we present evidence that chronic exposure to METH combined with brain infection by EcoHIV results in enhanced proliferation of NPCs in the subventricular zone (SVZ) in mice. Read More

View Article and Full-Text PDF

Imaging superiority of [Ga]-FAPI-04 over [F]-FDG PET/CT in alveolar soft part sarcoma (ASPS).

Eur J Nucl Med Mol Imaging 2021 May 13. Epub 2021 May 13.

Key laboratory of Carcinogenesis and Translational Research (Ministry of Education/Beijing), NMPA Key Laboratory for Research and Evaluation of Radiopharmaceuticals (National Medical Products Administration), Department of Nuclear Medicine, Peking University Cancer Hospital & Institute, No. 52 Fucheng Rd., Beijing, 100142, China.

View Article and Full-Text PDF

A Comprehensive Phylogenomic Platform for Exploring the Angiosperm Tree of Life.

Syst Biol 2021 May 13. Epub 2021 May 13.

Royal Botanic Gardens, Kew, Richmond, Surrey, TW9 3AE, United Kingdom.

The tree of life is the fundamental biological roadmap for navigating the evolution and properties of life on Earth, and yet remains largely unknown. Even angiosperms (flowering plants) are fraught with data gaps, despite their critical role in sustaining terrestrial life. Today, high-throughput sequencing promises to significantly deepen our understanding of evolutionary relationships. Read More

View Article and Full-Text PDF

Gut microbiota mediated the protective effects of tuna oil on collagen-induced arthritis in mice.

Food Funct 2021 May 13. Epub 2021 May 13.

State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Ningbo University, Ningbo, China. and School of Marine Science, Ningbo University, Ningbo, China.

Rheumatoid arthritis is emerging as a chronic autoimmune disease worldwide. In this study, the beneficial effects of tuna oil (TO) on collagen-induced arthritis (CIA) mice were investigated. Dietary administration of TO relieved arthritis severity and joint bone erosion, and ameliorated systemic inflammation. Read More

View Article and Full-Text PDF

Wedelolactone improves the renal injury induced by lipopolysaccharide in HK-2 cells by upregulation of protein tyrosine phosphatase non-receptor type 2.

J Int Med Res 2021 May;49(5):3000605211012665

Department of Critical Care Medicine, Beijing Friendship Hospital Affiliated to Capital Medical University, Beijing, China.

Objective: To explore the effects of wedelolactone (WEL) on sepsis-induced renal injury in the human renal proximal tubular epithelial cell line HK-2.

Methods: HK-2 cells were stimulated by 1 µg/ml lipopolysaccharide (LPS) to trigger renal injury . HK-2 cells were pretreated with or without WEL (0. Read More

View Article and Full-Text PDF

Quenching Mechanism of Uranyl(VI) by Chloride and Bromide in Aqueous and Non-Aqueous Solutions.

J Phys Chem A 2021 May 13. Epub 2021 May 13.

Institute of Chemistry, University of Potsdam, Karl-Liebknecht-Str. 24-25, D-14476 Potsdam, Germany.

A major hindrance in utilizing uranyl(VI) luminescence as a standard analytical tool, for example, in environmental monitoring or nuclear industries, is quenching by other ions such as halide ions, which are present in many relevant matrices of uranyl(VI) speciation. Here, we demonstrate through a combination of time-resolved laser-induced fluorescence spectroscopy, transient absorption spectroscopy, and quantum chemistry that coordinating solvent molecules play a crucial role in U(VI) halide luminescence quenching. We show that our previously suggested quenching mechanism based on an internal redox reaction of the 1:2-uranyl-halide-complex holds also true for bromide-induced quenching of uranyl(VI). Read More

View Article and Full-Text PDF