274 results match your criteria nuc rdna


, the first known rhizomycelial genus and species in the Rhizophydiales, is closely related to .

Mycologia 2021 Apr 13:1-7. Epub 2021 Apr 13.

Department of Ecology and Evolutionary Biology, University of Michigan, Ann Arbor, Michigan 48109-1085.

Molecular and ultrastructural investigations of the Chytridiomycota during the last 20 years have led to the separation of new orders, including the Rhizophydiales. Most species in this order are morphologically similar, possessing monocentric, eucarpic, spherical thalli. Here, based on analysis of nuc 18S and 28S rDNA, we add the new genus and species to the order. Read More

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and : Two new truffles encased in tomentose mycelium from Japan.

Mycologia 2021 Apr 9:1-11. Epub 2021 Apr 9.

Graduate School of Frontier Sciences, The University of Tokyo, Kashiwanoha, Kashiwa City, Chiba 277-8563, Japan.

We describe two new Japanese truffle species, and , based on molecular and morphological analyses. Both species are clearly distinguishable from other species by the ocher tomentose mycelium covering the ascoma surfaces. has one-spored asci that each contain a large (68-97 × 51-80 µm), reddish-brown ascospore; these microscopic characters are similar to those of closely related Chinese species, , and forms one to four ascospores per ascus with a reddish-brown color similar to that of ascospores of and , although their spores are much larger than those of (27‒64 × 26‒55 µm). Read More

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is a fast polysaccharide producer among species.

Mycologia 2021 Mar 25:1-12. Epub 2021 Mar 25.

Centro de Recursos Renovables de la Zona Semiárida (CERZOS), Universidad Nacional del Sur-CONICET, Camino La Carrindanga Km7, B8000 Bahía Blanca, Buenos Aires, Argentina.

The selection of fast-growing and high-yield-producing strains is required to satisfy the market demand on fungal food supplements. To that aim, three strains deposited in our collection as and were screened for polysaccharide production and biomass yield. strains deposited as were identified as and by nuc rDNA internal transcribed spacer ITS1-5. Read More

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Four new species of sequestrate from Chilean Nothofagaceae forests.

Mycologia 2021 Mar 2:1-14. Epub 2021 Mar 2.

Department of Plant Pathology, University of Florida, P.O. Box 110680, Gainesville, Florida 32611.

Sequestrate fungi have enclosed hypogeous, subhypogeous, or epigeous basidiomes and have lost the ability to actively discharge their spores. They can be distinguished as gasteroid (basidiome fully enclosed with a loculated hymenophore) or secotioid (basidiome with some agaricoid or pileate-stipitate features, but the lamellae are misshapen and unexposed or mostly unexposed at maturity). There are only four reports of sequestrate taxa within the ectomycorrhizal family Inocybaceae, three from Australia and one from western North America. Read More

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Delimitation and phylogeny of , and introduction of and , genera nova.

Mycologia 2021 Mar-Apr;113(2):390-433. Epub 2021 Feb 17.

Westerdijk Fungal Biodiversity Institute, 3508 AD Utrecht, The Netherlands.

(Chaetosphaeriaceae) is a phialidic dematiaceous hyphomycete with teleomorphs classified in . It is associated with significant variability of asexual morphological traits, which led to its broad delimitation. In the present study, six loci: nuc rDNA internal transcribed spacer region ITS1-5. Read More

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February 2021

Two new species of Chytriomycetaceae: Morphological, phylogenetic, and ultrastructural characterization.

Mycologia 2021 Mar-Apr;113(2):312-325. Epub 2021 Feb 4.

Núcleo de Pesquisa em Micologia, Instituto de Botânica, Av. Miguel Stéfano 3687, São Paulo, São Paulo, Brazil.

Systematics of Chytridiales has been deeply influenced by analyses of molecular loci and zoospore ultrastructure. Even though the Chytridiales is the largest order within Chytridiomycota, Brazilian isolates of this clade have been poorly integrated. Here, we isolated seven species and documented their morphology, including zoospore ultrastructure for , and phylogenetic positions for all based on analyses of nuc 18S and 28S rDNA. Read More

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February 2021

A revision of section with nine new species from China.

Mycologia 2021 Jan-Feb;113(1):191-211. Epub 2020 Dec 16.

State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences , Chaoyang District, Beijing 100101, China.

sect. includes numerous species that are potential candidates for cultivation, and some have high nutritional and medicinal interests. Between 2012 and 2017, 147 specimens of . Read More

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December 2020

sp. nov. a new member of the Cystofilobasidiales from soil in China, and description of the teleomorphic-stage of .

MycoKeys 2020 20;74:75-90. Epub 2020 Oct 20.

School of Life Science and Technology, Nanyang Normal University, Nanyang 473061, China Nanyang Normal University Nanyang China.

In a study on the fungal diversity in Northeast China, twelve yeast isolates were obtained from soils collected in three provinces, Helongjiang, Jilin and Liaoning. Morphological assessment and phylogenetic analyses of the nuc rDNA internal transcribed spacer (ITS) region and the D1/D2 domains of the nuc 28S rDNA (nuc 28S) gene of the 12 cultures placed them in the genus , namely , , , and . A total of three isolates represented a hitherto undescribed species, which is described here as (MB 834813). Read More

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October 2020

, a new entomophthoralean species isolated from leafcutter ants.

Mycologia 2021 Jan-Feb;113(1):56-64. Epub 2020 Nov 5.

Ants Laboratory, Department of Science and Technology, Quilmes National University , CONICET, Roque Saenz Peña 352, Bernal B1876BXD, Buenos Aires, Argentina.

Entomophthoralean fungi with pathogenic abilities to infect social insects are rare. Here, we describe a fungus isolated from leafcutter ants. Morphologically, the fungus has spherical primary conidia and two types of microconidia: one with the same shape as the primary conidia and another with an elliptical to half-moon shape. Read More

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November 2020

New species of associated with Australian terrestrial orchids in the Cryptostylidinae and Drakaeinae.

Mycologia 2021 Jan-Feb;113(1):212-230. Epub 2020 Nov 4.

Ecology and Evolution, Research School of Biology, The Australian National University , Canberra, ACT 2601, Australia.

Many orchids have an obligate relationship with mycorrhizal fungi for seed germination and support into adulthood. Despite the importance of as mycorrhizal partners, many species remain undescribed. Here, we use multiple sequence locus phylogenetic analyses to delimit and describe six new species associated with Australian terrestrial orchids from the subtribes Cryptostylidinae and Drakaeinae. Read More

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November 2020

, a new species of the genus infecting Polygonaceae in Gansu, China.

Mycologia 2021 Jan-Feb;113(1):78-91. Epub 2020 Oct 30.

State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences , Beijing 100101, China.

A new species, , was isolated from several species of Polygonaceae in 2011 and 2012 in Tongwei County, Gansu Province, China. The species infects , and , causing brown leaf spots and large blotches with concentric rings in the field. is morphologically characterized by conidia spherical, unicellular, hyaline to pale brown or brown, (10. Read More

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October 2020

Development and application of a triplex-PCR assay for rapid detection of methicillin-resistant Staphylococcus aureus from pigs.

Lett Appl Microbiol 2021 Feb 10;72(2):121-125. Epub 2020 Nov 10.

Indian Veterinary Research Institute, Izatnagar, UP, India.

A triplex-PCR assay was developed and evaluated for rapid detection of methicillin-resistant Staphylococcus aureus (MRSA) recovered from various biological samples of pig. Three sets of primers were designed to target mecA, 16S rRNA and nuc genes of MRSA. The specific amplification generated three bands on agarose gel, with sizes 280 bp for mecA, 654 bp for 16S rRNA and 481 bp for nuc, respectively. Read More

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February 2021

Cultivable marine fungi from the Arctic Archipelago of Svalbard and their antibacterial activity.

Mycology 2019 Dec 27;11(3):230-242. Epub 2019 Dec 27.

Marbio, The Norwegian College of Fishery Science, Department at Faculty of Biosciences, Fisheries and Economics, UiT The Arctic University of Norway, Tromsø, Norway.

During a research cruise in 2016, we isolated fungi from sediments, seawater, driftwood, fruiting bodies, and macroalgae using three different media to assess species richness and potential bioactivity of cultivable marine fungi in the High Arctic region. Ten stations from the Svalbard archipelago (73-80 °N, 18-31 °E) were investigated and 33 fungal isolates were obtained. These grouped into 22 operational taxonomic units (OTUs) using nuc rDNA internal transcribed spacer regions (ITS1-5. Read More

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December 2019

Marasmioid rhizomorphs in bird nests: Species diversity, functional specificity, and new species from the tropics.

Mycologia 2020 Nov-Dec;112(6):1086-1103. Epub 2020 Sep 8.

Department of Botany and Plant Pathology, Purdue University , West Lafayette, Indiana 47907.

In tropical and subtropical rainforests, vegetative fungal rhizomorphs from the Marasmiineae are routinely used as construction material in bird nests. Because rhizomorphs seldom produce mushrooms within nests, the fungal species involved remain largely unknown. In turn, this limitation has prevented us from resolving broader questions such as whether specific fungal species are selected by birds for different functional roles (i. Read More

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September 2020

: An overlooked lineage of diverse endophytes.

Stud Mycol 2020 Mar 13;95:293-380. Epub 2020 Mar 13.

Ottawa Research and Development Centre, Biodiversity (Mycology and Microbiology), Agriculture and Agri-Food Canada, 960 Carling Avenue, Ottawa, Ontario, K1A 0C6, Canada.

is a taxonomically neglected discomycete genus () of commonly encountered saprotrophs on decaying plant tissues throughout temperate regions. The combination of indistinct morphological characters, more than 700 names in the literature, and lack of reference DNA sequences presents a major challenge when working with . Unidentified endophytes, including strains that produced antifungal or antiinsectan secondary metabolites, were isolated from conifer needles in New Brunswick and placed with uncertainty in and , necessitating a more comprehensive treatment of these genera. Read More

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, gen. nov., a new oomycete lineage with isogamous sexual reproduction.

Mycologia 2020 Sep-Oct;112(5):989-1002. Epub 2020 Aug 26.

Department of Biology, Randolph-Macon College , Ashland, Virginia 23005.

This paper describes the taxonomy, developmental morphology, and phylogeny of , a new monotypic member of the Leptomitales (Oomycota). In phylogenetic trees inferred from concatenated and separate nuc 18S rDNA (18S) and nuc 28S rDNA (28S) sequences, forms a well-supported clade related to but distinct from , a member of the Leptomitales. The organism is a holocarpic facultative saprotroph of moribund aquatic insects but grows well on a variety of mycological media in which it produces large eucarpic hyphae with a peripheral layer of protoplasm surrounding a central vacuole. Read More

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sp. nov., a new member of the ' group' isolated from healthy black bears.

Int J Syst Evol Microbiol 2020 Aug;70(8):4637-4645

Department of Biomedical and Diagnostic Sciences, University of Tennessee College of Veterinary Medicine, Knoxville, Tennessee, USA.

Six strains were isolated from healthy black bears () in the Great Smoky Mountains National Park, Tennessee, USA. Phylogenetic analysis based on complete genome, 16S rRNA, , , and genes, and MALDI-TOF-MS main spectral profiles revealed that the strains belonged to one species and showed the closest relatedness to members of the ' group' (SIG), which include , and . The strains were positive in SIG-specific and negative in individual species-specific PCR assays for the gene. Read More

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Pyrophilous fungi detected after wildfires in the Great Smoky Mountains National Park expand known species ranges and biodiversity estimates.

Mycologia 2020 Jul-Aug;112(4):677-698. Epub 2020 Jun 4.

Department of Plant and Microbial Biology, University of California , Berkeley, California 94520-3102.

Following a late fall wildfire in 2016 in the Great Smoky Mountains National Park, pyrophilous fungi in burn zones were documented over a 2-y period with respect to burn severity and phenology. Nuc rDNA internal transcribed spacer (ITS1-5.8S-ITS2 = ITS) barcodes were obtained to confirm morphological evaluations. Read More

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Taxonomic revision of species of and on , including two new species from China.

Mycologia 2020 Jul-Aug;112(4):742-752. Epub 2020 May 29.

Faculty of Education, Ibaraki University , Ibaraki 310-8512, Japan.

and are the only species of genus that parasitize plants belonging to genus (Rosaceae). Systematic revision of the genus and related genera by molecular phylogenetic analyses using nuc rDNA internal transcribed spacer (5.8S-ITS2 = ITS2) and nuc rDNA 28S (28S) sequences indicated that and belong to a group of species that also occur on . Read More

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Phylogenetic placement of and reconsideration of .

Fungal Syst Evol 2020 Jun 11;5:119-129. Epub 2019 Oct 11.

Department of Plant Pathology, National Chung Hsing University, Taichung 40227, Taiwan.

is a large conspicuous polypore fungus growing on dead or living angiosperm trees in subtropical-boreal areas of China, Indonesia, Japan, and Taiwan. The present study places in the family that belongs to the order within (), based on evidence derived from morphological and ecological characteristics, and phylogenetic analyses of sequences of nuclear rDNA regions (5.8S, nuc 18S, nuc 28S) and protein-coding genes (, and ). Read More

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Bambusicolous revisited: multilocus phylogeny reveals a clade of host-exclusive species.

Mycologia 2020 May-Jun;112(3):633-648. Epub 2020 May 15.

Programa de Pós-Graduação em Botânica, Laboratório de Micologia, Departamento de Botânica, Universidade Federal do Rio Grande do Sul, Campus do Vale , CEP: 91501-970, Porto Alegre, Rio Grande do Sul, Brazil.

Fomitiporia: is a genus of wood-inhabiting Hymenochaetaceae (Agaricomycetes) that includes pathogens and decomposers occurring on a wide array of plant substrates. Some species decay culms of woody bamboos, a poorly known biotic interaction. Four bambusicolous species of are currently known. Read More

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, sp. nov., a newly described rust on the federally endangered plant, California sea-blite ().

Mycologia 2020 May-Jun;112(3):543-551. Epub 2020 May 6.

Foreign Disease-Weed Science Research Unit, Agricultural Research Service , United States Department of Agriculture, Fort Detrick, Maryland 21714.

Rust disease was observed on populations of near Morro Bay, California. The pathogen was identified as a species of based on teliospore and urediniospore morphology and nuc 28S rDNA sequence analysis. The isolate was compared with previously described species of that infect members of Chenopodiaceae, prompting a taxonomic reevaluation of species on . Read More

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, a new species from Turkey, and a multigene phylogeny of .

Mycologia 2020 May-Jun;112(3):577-587. Epub 2020 Apr 24.

Vocational School of Health Services, Süleyman Demirel University , 32260 Isparta, Turkey.

A new species of , collected from Aydın Province on the coast of the Aegean Sea in southwestern Turkey, is described as , sp. nov., based on morphology and multigene molecular analysis of three nuc rDNA gene regions: internal transcribed spacer ITS1-5. Read More

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Purple haze: Cryptic purple sequestrate in New Zealand.

Mycologia 2020 May-Jun;112(3):588-605. Epub 2020 Apr 21.

Department of Botany, University of Otago , PO Box 56, Dunedin 9054, New Zealand.

Cortinarius: is a species-rich ectomycorrhizal genus containing taxa that exhibit agaricoid or sequestrate basidiome morphologies. In New Zealand, one of the most recognizable and common species is the purple sequestrate fungus, . We used genome skimming of the almost 100-y-old type specimen from to obtain the nuc rDNA internal transcribed spacer region ITS1-5. Read More

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Hydnaceous fungi of China 8. Morphological and molecular identification of three new species of and a new record from southwest China.

MycoKeys 2020 3;66:83-103. Epub 2020 Apr 3.

CAS Key Laboratory of Forest Ecology and Management, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110164, China Institute of Applied Ecology, Chinese Academy of Sciences Shenyang China.

Three new stipitate hydnoid fungi, , and , are described and illustrated, based on morphological characteristics and nuc ITS rDNA + nuc LSU rDNA sequence analyses and a new record, , from China is reported. is characterised by ellipsoid to round basidiocarps, reddish-brown to dark brown, felted pileal surface with white and incurved margins, simple-septate and partly short-celled generative hyphae and irregular subglobose, thin-walled, brown basidiospores with tuberculate ornamentation (tuberculi up to 1 μm long). is characterised by infundibuliform to round, occasionally deeply fissured pileus, pale orange to dark ruby pileal surface with ascending and coarse scales, simple-septate generative hyphae and irregular ellipsoid to globose, thin-walled, brown basidiospores with tuberculate ornamentation (tuberculi up to 0. Read More

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is a new species of parasitic chytrid of the commercially grown alga, .

Mycologia 2020 May-Jun;112(3):606-615. Epub 2020 Apr 9.

Department of Ecology and Evolutionary Biology, University of Michigan , Ann Arbor, Michigan 48109-1085.

Aquaculture companies grow the green alga (Chlorophyta) to extract the carotenoid astaxanthin to sell, which is used as human and animal dietary supplements. We were requested to identify an unknown pathogen of from an alga growing facility in the southwestern United States. To identify this zoosporic fungus and determine its phylogenetic placement among other chytrids, we isolated it into pure culture, photographed its morphology and zoospore ultrastructure, and sequenced and analyzed portions of nuc rDNA 18S and 28S genes. Read More

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Multiple evolutionary origins of sequestrate species in the agaricoid genus .

Mycologia 2020 Mar-Apr;112(2):400-422. Epub 2020 Mar 9.

Institute of Microbiology, Friedrich Schiller University Jena, Neugasse 25, 07743 Jena, Germany.

accommodates lamellate agaricoid species as well as sequestrate angiocarpic taxa, which do not form a monophyletic lineage within the genus. To clarify phylogenetic affinities and delimit species boundaries among sequestrate representatives of the genus, we analyzed historical and contemporary material from a broad geographic range, encompassing North America, southern Africa, eastern Asia, the Greek and Iberian peninsulas, and the Mediterranean islands of Cyprus and Lesvos. Six sequestrate lineages of were identified, which appear to have evolved in at least three independent gasteromycetation events. Read More

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, and in China.

Mycologia 2020 Mar-Apr;112(2):371-399. Epub 2020 Mar 2.

State Key Laboratory for Conservation and Utilization of Bioresources in Yunnan, Yunnan University, Kunming 650091, China.

Within the family Entolomataceae, many reports of and have been published, but few reports on and allied genera are available. In this study, , and from China were studied by morphological and phylogenetic methods. Portions of nuc 28S rDNA (28S), RNA polymerase II second largest subunit (), translation elongation factor 1-alpha (), and ATPase subunit 6 (), were employed to elucidate the relationships of and allies. Read More

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Expansion of the genus in North America to include , sp. nov., and , comb. nov.

Mycologia 2020 Mar-Apr;112(2):423-437. Epub 2020 Feb 19.

Herbarium, Department of Cell, Molecular, and Microbiology, University of South Florida, Tampa, Florida 33620.

is a small genus of Boletaceae found primarily in the Northern Hemisphere characterized by the light to dark brown hymenophore that stains blue. In Florida, specimens of were collected that resembled , a species known primarily from northeastern North America and Europe. Five nuclear loci of these Florida specimens were sequenced and phylogenetically analyzed. Read More

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New species and reports of from northern North America compared with related Eurasian species.

Mycologia 2020 Mar-Apr;112(2):438-452. Epub 2020 Feb 19.

Department of Biological and Environmental Sciences and Gothenburg Global Biodiversity Centre, University of Gothenburg, P.O. Box 461, SE 405 30 Göteborg, Sweden.

This study describes four gray or brown species of (Hygrophoraceae, Agaricales), two of them new species, restricted to arctic-alpine and northern boreal zones of North America, and relates them morphologically and phylogenetically using multigene and nuc rDNA internal transcribed spacer ITS1-5.8S-ITS (ITS barcode) analyses to their similar, known counterparts. , epitypified here, is shown to be a pan-palearctic species with sequence-confirmed collections from Fennoscandia and easternmost Asia. Read More

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