11,174 results match your criteria nsc


Large seasonal fluctuations in whole-tree carbohydrate reserves: is storage more dynamic in boreal ecosystems?

Ann Bot 2021 Jul 22. Epub 2021 Jul 22.

Department of Renewable Resources, University of Alberta, Edmonton, AB Canada.

Background And Aims: Carbon reserves are a critical source of energy and substrates that allow trees to cope with periods of minimal carbon gain and/or high carbon demands, conditions which are prevalent in high latitude forests. However, we have a poor understanding of carbon reserve dynamics at the whole-tree level in mature boreal trees. We therefore sought to quantify the seasonal changes in whole-tree and organ level carbon reserve pools in mature boreal Betula papyrifera. Read More

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Lisdexamfetamine Pharmacokinetic Comparison Between Patients Who Underwent Roux-en-Y Gastric Bypass and Nonsurgical Controls.

Obes Surg 2021 Jul 21. Epub 2021 Jul 21.

Department of Pharmaceutics, College of Pharmacy, University of Minnesota, Minneapolis, MN, USA.

Introduction/purpose: The objective of this research was to characterize the impact of Roux-en-Y gastric bypass (RYGB) on the pharmacokinetic properties of the pro-drug lisdexamfetamine and its active metabolite, d-amphetamine.

Materials And Methods: A case-control design was used where patients who had undergone RYGB 9-24 months prior were matched on sex, age, and body mass index (BMI) to nonsurgical controls who had no history of weight loss surgery. Each participant received a single 50 mg dose of lisdexamfetamine, and plasma samples were collected over a 24-h period following dosing. Read More

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The pyriproxyfen metabolite, 4'-OH-PPF, disrupts thyroid hormone signaling in neural stem cells, modifying neurodevelopmental genes affected by ZIKA virus infection.

Environ Pollut 2021 Jul 13:117654. Epub 2021 Jul 13.

Laboratory Molecular Physiology and Adaptation, CNRS UMR 7221, Muséum National d'Histoire Naturelle, Department Adaptations of Life, F-75005, Paris, France. Electronic address:

North-Eastern Brazil saw intensive application of the insecticide pyriproxyfen (PPF) during the microcephaly outbreak caused by the Zika virus (ZIKV). ZIKV requires the neural RNA-binding protein Musashi-1 to replicate. Thyroid hormone (TH) represses MSI1. Read More

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Generation of Transgenic Rat Embryonic Stem Cells Using the CRISPR/Cpf1 System for Inducible Gene Knockout.

Biochemistry (Mosc) 2021 Jul;86(7):843-851

Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, 630090, Russia.

Rat embryonic stem cells (ESCs) play an important role in the studies of genes involved in maintaining of pluripotent state and early development of this model organism. To study functions of the essential genes, as well as the processes of cell differentiation, the method of induced knockout is widely used. The CreERT2/loxP system allows obtaining an inducible knockout in cells expressing tamoxifen-inducible Cre recombinase (CreERT2) and containing loxP sites flanking the target gene by adding 4-hydroxy tamoxifen to the culture medium. Read More

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Optic Nerve Engraftment of Neural Stem Cells.

Invest Ophthalmol Vis Sci 2021 Jul;62(9):30

Hamilton Glaucoma Center, Shiley Eye Institute and Viterbi Family Department of Ophthalmology, University of California San Diego, La Jolla, California, United States.

Purpose: To evaluate the integrative potential of neural stem cells (NSCs) with the visual system and characterize effects on the survival and axonal regeneration of axotomized retinal ganglion cells (RGCs).

Methods: For in vitro studies, primary, postnatal rat RGCs were directly cocultured with human NSCs or cultured in NSC-conditioned media before their survival and neurite outgrowth were assessed. For in vivo studies, human NSCs were transplanted into the transected rat optic nerve, and immunohistology of the retina and optic nerve was performed to evaluate RGC survival, RGC axon regeneration, and NSC integration with the injured visual system. Read More

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Tracking the Differentiation Status of Human Neural Stem Cells through Label-Free Raman Spectroscopy and Machine Learning-Based Analysis.

Anal Chem 2021 Jul 20. Epub 2021 Jul 20.

Department of Biological Engineering, Utah State University, 4105 Old Main Hill, ENGR 402, Logan, Utah 84322, United States.

The ability to noninvasively monitor stem cells' differentiation is important to stem cell studies. Raman spectroscopy is a non-harmful imaging approach that acquires the cellular biochemical signatures. Herein, we report the first use of label-free Raman spectroscopy to characterize the gradual change during the differentiation process of live human neural stem cells (NSCs) in the in vitro cultures. Read More

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Evaluating the results of long tubular bone distraction with an advanced rod monolateral external fixator for achondroplasia.

Sci Rep 2021 Jul 19;11(1):14727. Epub 2021 Jul 19.

Department of Pediatrician Surgery, NSC Astana Medical University, Nur-Sultan, Kazakhstan.

The work aimed to evaluate the effectiveness of the developed distraction system based on the rod external monolateral fixation mechanisms by comparing it with the classical technique of long tubular bones distraction based on the circular multi-axial system. The study included patients with a genetically confirmed diagnosis of achondroplasia. The experimental group consisted of 14 patients who underwent surgical limb lengthening by the rod monolateral external fixator with a distraction system developed by the authors. Read More

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Relative Traffic Tolerance of Warm-Season Grasses and Suitability for Grazing by Equine.

J Equine Vet Sci 2021 Aug 7;103:103244. Epub 2020 Oct 7.

Department of Animal and Avian Sciences, The University of Maryland, College Park, MD. Electronic address:

Warm season wear-tolerant turfgrasses, such as those used on golf courses and athletic fields, may be valuable forages on equine operations because of their potential to remain viable during heavy hoof traffic. Crabgrass may also be suitable as it thrives in conditions where other grasses have limited success. The objective of this study was to assess the relative traffic tolerance and nutritional composition of five warm-season (WS) turfgrass cultivars of bermudagrass and zoysiagrass and one WS forage-type crabgrass. Read More

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Alterations in Source-Sink Relations Affect Rice Yield Response to Elevated CO: A Free-Air CO Enrichment Study.

Front Plant Sci 2021 2;12:700159. Epub 2021 Jul 2.

Key Laboratory of Crop Genetics and Physiology of Jiangsu Province, Yangzhou University, Yangzhou, China.

To understand the effects of source-sink relationships on rice yield response to elevated CO levels (eCO), we conducted a field study using a popular cultivar grown in a free-air CO enrichment environment in 2017-2018. The source-sink ratio of rice was set artificially via source-sink treatments (SSTs) at the heading stage. Five SSTs were performed in 2017 (EXP1): cutting off the flag leaf (LC1) and the top three functional leaves (LC3), removing one branch in every three branches of a panicle (SR1/3) and one branch in every two branches of a panicle (SR1/2), and the control (CK) without any leaf cutting or spikelet removal. Read More

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LncRNA MEG3 inhibits the proliferation of neural stem cells after ischemic stroke via the miR-493-5P/MIF axis.

Biochem Biophys Res Commun 2021 Jul 14;568:186-192. Epub 2021 Jul 14.

Department of Forensic Medicine, Chongqing Medical University, #1 Yixueyuan Road, Chongqing, 400016, China. Electronic address:

Objective: The proliferation of neural stem cells (NSCs), or lack thereof, can have profound effects on brain tissue remodeling for ischemic stroke (IS). In this study, we aimed to reveal the influence of the lncRNA MEG3/miR-493-5p/MIF axis on NSC proliferation after IS.

Methods: We established an oxygen glucose-deprivation/reoxygenation (OGD/R) in vitro model of IS in NSCs. Read More

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AP sites in various mRNA positions cross-link to the protein uS3 in the translating mammalian ribosome.

Biochim Biophys Acta Proteins Proteom 2021 Jul 14;1869(10):140698. Epub 2021 Jul 14.

Institute of Chemical Biology and Fundamental Medicine SB RAS, Novosibirsk 630090, Russia. Electronic address:

Abasic (AP) sites in mRNAs are lesions whose accumulation in cells is linked to various neurodegenerative diseases arising from the appearance of truncated peptides due to the premature cessation of translation of these mRNAs. It is believed that the translation of AP site-containing mRNAs is stopped when the damaged codon arrives to the A site, where it is not decoded. We propose an alternative translation arrest mechanism mediated by the 40S ribosomal subunit protein uS3. Read More

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University of Southern California and buck institute nathan shock center: multidimensional models of aging.

Geroscience 2021 Jul 16. Epub 2021 Jul 16.

Leonard Davis School of Gerontology, University of Southern California, Los Angeles, USA.

The USC-Buck Nathan Shock Center of Excellence in the Biology of Aging is a new and fully integrated multi-institutional center focused on training the next generation of geroscientists and providing access to cutting-edge geroscience technologies to investigators across the nation. The USC-Buck NSC is devoted to forging a deeper understanding of how and why aging processes cause disease in order to advance the translation of basic research on aging into effective preventions and therapies. Including more than 61 NIA-supported investigators, six NIA-funded research centers, four NIA T32s, and several additional aging research centers of excellence, the USC-Buck NSC constitutes one of the largest collections of leaders in geroscience research within the USA; the unique nature of the USC-Buck NSC research infrastructure ensures an integrated organization that is representative of the wide breadth of topics encompassed by the biology of aging field. Read More

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Impaired generation of mature neurons due to extended expression of Tlx by repressing Sox2 transcriptional activity.

Stem Cells 2021 Jul 16. Epub 2021 Jul 16.

Jiangsu Key Laboratory of Xenotransplantation, Nanjing Medical University, Nanjing, People's Republic of China.

As a master regulator of the dynamic process of adult neurogenesis, timely expression and regulation of the orphan nuclear receptor Tailless (Tlx) is essential. However, there is no study yet to directly investigate the essential role of precise spatiotemporal expressed Tlx. Here, we generated a conditional gain of Tlx expression transgenic mouse model, which allowed the extended Tlx expression in neural stem cells (NSCs) and their progeny by mating with a TlxCreER mouse line. Read More

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Differential Regenerative Capacity of the Optic Tectum of Adult Medaka and Zebrafish.

Front Cell Dev Biol 2021 29;9:686755. Epub 2021 Jun 29.

Functional Biomolecular Research Group, Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology, Osaka, Japan.

Zebrafish have superior regenerative capacity in the central nervous system (CNS) compared to mammals. In contrast, medaka were shown to have low regenerative capacity in the adult heart and larval retina, despite the well-documented high tissue regenerative ability of teleosts. Nevertheless, medaka and zebrafish share similar brain structures and biological features to those of mammals. Read More

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Glioblastoma Proximity to the Lateral Ventricle Alters Neurogenic Cell Populations of the Subventricular Zone.

Front Oncol 2021 29;11:650316. Epub 2021 Jun 29.

Department of Neurosurgery, Mayo Clinic, Jacksonville, FL, United States.

Despite current strategies combining surgery, radiation, and chemotherapy, glioblastoma (GBM) is the most common and aggressive malignant primary brain tumor in adults. Tumor location plays a key role in the prognosis of patients, with GBM tumors located in close proximity to the lateral ventricles (LVs) resulting in worse survival expectancy and higher incidence of distal recurrence. Though the reason for worse prognosis in these patients remains unknown, it may be due to proximity to the subventricular zone (SVZ) neurogenic niche contained within the lateral wall of the LVs. Read More

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Boosting the lithium storage performance by synergistically coupling ultrafine heazlewoodite nanoparticle with N, S co-doped carbon.

J Colloid Interface Sci 2021 Jul 8;604:368-377. Epub 2021 Jul 8.

Shanghai Key Laboratory of Magnetic Resonance, School of Physics and Electronic Science, East China Normal University, Shanghai 200062, China. Electronic address:

Transition metal sulfides, as an important class of inorganics, have been shown to be potential high-performance electrode candidates for lithium-ion batteries (LIBs) in account of their high activity towards lithium storage, rich types and diverse structures. Despite these advantages, structure degradation related with volume variations upon electrochemical cycling restricts their further development. In this present study, a unique hybrid structure with ultrafine heazlewoodite nanoparticles (less than 10 nm) in-situ confined in nitrogen and sulfur dual-doped carbon ([email protected]) was constructed though a facile pyrolysis process, using a novel Ni-based metal chelates as the precursor. Read More

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The role of gold nanoparticles' aspect ratio in plasmon-enhanced luminescence and the singlet oxygen generation rate of Mo clusters.

Chem Commun (Camb) 2021 Jul 15. Epub 2021 Jul 15.

Scientific Institute of Clinical and Experimental Lymphology-branch of ICG SB RAS, 2 Timakova str., Novosibirsk 630060, Russian Federation.

Here we present a study on the effect of the aspect ratio (AR) of gold nanoparticles on the emission intensity and singlet oxygen production rate of hexamolybdenum cluster-doped silica particles. It was shown that these parameters can be enhanced gradually up to 6.7- and 13-fold with the AR. Read More

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Biomass-derived N,S co-doped 3D multichannel carbon supported [email protected]@Pt catalysts for oxygen reduction.

Environ Res 2021 Jul 11;202:111684. Epub 2021 Jul 11.

College of Chemical Engineering, Beijing University of Chemical Technology, Beijing, 100029, China.

A beancurd-derived mesoporous carbon (NSC) was prepared by an environmentally friendly procedure, and then it was investigated as [email protected]@Pt core-shell catalysts support ([email protected]@Pt-NSC) for oxygen reduction reaction (ORR). The [email protected]@Pt-NSC (E = 0.91 V) has a marginally negative ORR half-wave potential compared with other materials, in particular Pt/C (E = 0. Read More

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Icariin Promotes Survival, Proliferation, and Differentiation of Neural Stem Cells In Vitro and in a Rat Model of Alzheimer's Disease.

Stem Cells Int 2021 23;2021:9974625. Epub 2021 Jun 23.

Department of Pharmacy, Xuanwu Hospital of Capital Medical University, Beijing 100053, China.

Alzheimer's disease (AD) involves the degeneration of cholinergic neurons in the basal forebrain. Neural stem cell (NSC) transplantation has emerged as a promising therapeutic approach for treating AD. Icariin (ICA) is the main active component in , a traditional Chinese herb. Read More

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Immunological Responses to Transgene-Modified Neural Stem Cells After Transplantation.

Front Immunol 2021 23;12:697203. Epub 2021 Jun 23.

Department of Neurosurgery, The First Affiliated Hospital of Shantou University Medical College, Guangdong, China.

Neural stem cell (NSC) therapy is a promising therapeutic strategy for stroke. Researchers have frequently carried out genetic modification or gene editing of stem cells to improve survival or therapeutic function. However, NSC transplantation carries the risk of immune rejection, and genetic modification or gene-editing might further increase this risk. Read More

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LncRNA and mRNA Expression Profiles in Methylprednisolone Stimulated Neural Stem Cells.

Front Neurosci 2021 23;15:669224. Epub 2021 Jun 23.

Department of Orthopedics, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, China.

Spinal cord injury (SCI) is a devastating neurological disorder that affects thousands of individuals each year. Previously, our study in non-human primates with SCI demonstrated that methylprednisolone (MP) resulted in the dysfunction of neural stem cells (NSCs), which may help to explain the controversial roles of MP in SCI. However, the detailed mechanism is still unclear. Read More

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Hydroxyapatite Nanorods Function as Safe and Effective Growth Factors Regulating Neural Differentiation and Neuron Development.

Adv Mater 2021 Jul 11:e2100895. Epub 2021 Jul 11.

State Key Laboratory of Crystal Materials, Shandong University, Jinan, 250100, P. R. China.

Neural stem cell (NSC) transplantation is one of the most promising therapeutic strategies for neurodegenerative diseases. However, the slow spontaneous differentiation of NSCs often hampers their application in neural repair. Although some biological growth factors accelerate the differentiation of NSCs, their high cost, short half-life, and unpredictable behavior in vivo, as well as the complexity of the operation, hinder their clinical use. Read More

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Progress in the characterization of insulin-like peptides in aphids: Immunohistochemical mapping of ILP4.

Insect Biochem Mol Biol 2021 Jul 9;136:103623. Epub 2021 Jul 9.

Institut de Biologia Integrativa de Sistemes, Parc Cientific, Universitat de València, C/ Catedràtic Agustín Escardino Benlloch, 9, 46980, Paterna, València, Spain. Electronic address:

Aphids were the first animals described as photoperiodic due to their seasonal switch from viviparous parthenogenesis to sexual reproduction (cyclical parthenogenesis) caused by the shortening of the photoperiod in autumn. This switch produces a single sexual generation of oviparous females and males that mate and lay diapausing cold-resistant eggs that can overcome the unfavourable environmental conditions typical of winter in temperate regions. Previous studies have hinted at a possible implication of two insulin-like peptides (ILP1 and ILP4) in the aphid seasonal response, changing their expression levels between different photoperiodic conditions. Read More

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A new approach to precise mapping of local temperature fields in submicrometer aqueous volumes.

Sci Rep 2021 Jul 9;11(1):14228. Epub 2021 Jul 9.

Prokhorov General Physics Institute of the Russian Academy of Sciences, Vavilov str. 38, Moscow, Russia, 119991.

Nanodiamonds hosting temperature-sensing centers constitute a closed thermodynamic system. Such a system prevents direct contact of the temperature sensors with the environment making it an ideal environmental insensitive nanosized thermometer. A new design of a nanodiamond thermometer, based on a 500-nm luminescent nanodiamond embedded into the inner channel of a glass submicron pipette is reported. Read More

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Identification of the gliogenic state of human neural stem cells to optimize in vitro astrocyte differentiation.

J Neurosci Methods 2021 Jul 7;361:109284. Epub 2021 Jul 7.

Neuroimmunology Lab, Experimental and Clinical Research Center (ECRC), Charité - Universitätsmedizin Berlin und Max Delbrueck Center for Molecular Medicine, 13125 Berlin, Germany. Electronic address:

Background: Human preclinical models are crucial for advancing biomedical research. In particular consistent and robust protocols for astrocyte differentiation in the human system are rare.

New Method: We performed a transcriptional characterization of human gliogenesis using embryonic H9- derived hNSCs. Read More

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Exposure to air pollution and incidence of atopic dermatitis in the general population: A national population-based retrospective cohort study.

J Am Acad Dermatol 2021 Jul 6. Epub 2021 Jul 6.

Department of Dermatology, Hanyang University Guri Hospital, College of Medicine, Hanyang University, Republic of Korea. Electronic address:

Background: To date, there is little evidence to determine whether atopic dermatitis (AD) can be caused by exposure to air pollutants including gases and particulate matter (PM).

Objective: We aimed to evaluate the relationship between air pollutants and incidence of AD using the National Health Insurance Service-National Sample Cohort (NHIS-NSC) database.

Methods: We included 209,168 subjects from the general population previously not diagnosed with AD from 2008 to 2013. Read More

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Seasonal and vertical characteristics of particulate and elemental concentrations along diverse street canyons in South India.

Environ Sci Pollut Res Int 2021 Jul 8. Epub 2021 Jul 8.

Space Physics Laboratory, Vikram Sarabhai Space Centre, Thiruvananthapuram, Kerala, 695022, India.

The impact of street geometries on vertical dispersion of PMs (PM and PM) in (1) non-street canyon (NSC), (2) street canyon (SC), and (3) street canyon with viaduct (SCV) was studied during four seasons. The chemical composition of the species was analysed for source apportionment. The mass concentration of PMs in canyons was in the order of SCV > SC > NSC, implicating the canyon effect. Read More

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Neurogenesis Potential Evaluation and Transcriptome Analysis of Fetal Hypothalamic Neural Stem/Progenitor Cells With Prenatal High Estradiol Exposure.

Front Genet 2021 22;12:677935. Epub 2021 Jun 22.

Center for Reproductive Medicine, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.

High maternal estradiol is reported to induce metabolic disorders by modulating hypothalamic gene expression in offspring. Since neurogenesis plays a crucial role during hypothalamus development, we explored whether prenatal high estradiol exposure (HE) affects proliferation and differentiation of fetal hypothalamic neural stem/progenitor cells (NSC/NPCs) in mice and performed RNA sequencing to identify the critical genes involved. NSC/NPCs in HE mice presented attenuated cell proliferation but increased neuronal differentiation compared with control (NC) cells. Read More

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Protein tyrosine phosphatase Shp2 positively regulates cold stress-induced tyrosine phosphorylation of SIRPα in neurons.

Biochem Biophys Res Commun 2021 Jul 5;569:72-78. Epub 2021 Jul 5.

Department of Laboratory Sciences, Gunma University Graduate School of Health Sciences, 3-39-22 Showa-Machi, Maebashi, Gunma, 371-8514, Japan. Electronic address:

The membrane protein SIRPα is a cold stress-responsive signaling molecule in neurons. Cold stress directly induces tyrosine phosphorylation of SIRPα in its cytoplasmic region, and phosphorylated SIRPα is involved in regulating experience-dependent behavioral changes in mice. Here, we examined the mechanism of cold stress-induced SIRPα phosphorylation in vitro and in vivo. Read More

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Ecologically driven selection of nonstructural carbohydrate storage in oak trees.

New Phytol 2021 Jul 8. Epub 2021 Jul 8.

School of the Environment, Yale University, New Haven, CT, 06511, USA.

Leaf habit is a major axis of plant diversity that has consequences for carbon balance since the leaf is the primary site of photosynthesis. Nonstructural carbohydrates (NSCs) produced by photosynthesis can be allocated to storage and serve as a resiliency mechanism to future abiotic and biotic stress. However, how leaf habit affects NSC storage in an evolutionary context has not been shown. Read More

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