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Ethyl acetate fraction of () attenuates lead acetate induced testicular toxicity in male Wistar rats.

J Complement Integr Med 2021 May 12. Epub 2021 May 12.

Department of Pharmacology, Benjamin S. Carson (Snr.) School of Medicine, Babcock University, Ilishan-Remo, Ogun State, Nigeria.

Objectives: The use of in folklore medicine for the management of reproductive disorders has been poorly reported. We sought to investigate the protective potential of the ethyl acetate fraction of stem bark extract (EFSC) on lead acetate-induced (LA) testicular toxicity in male rats.

Methods: Animals during a 28 days treatment received dimethyl sulfoxide (DMSO, 0. Read More

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Morphological Analysis of Bone Deformities of the Distal Femur in Toy Poodles with Medial Patellar Luxation.

Vet Comp Orthop Traumatol 2021 May 12. Epub 2021 May 12.

Department of Clinical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, New York, United States.

Objective:  The aim of this study was to evaluate the bone deformities of the distal femur of Toy Poodles with medial patellar luxation (MPL) using computed tomography.

Methods:  Computed tomography data from 37 stifles of Toy Poodles (16 normal, 10 grade 2 MPL and 11 grade 4 MPL) were analysed. For trochlear morphology, sulcus angle, medial or lateral trochlear inclination and trochlear angle were compared. Read More

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Functional Anatomy of the Craniolateral and Caudomedial Bundles of the Caudal Cruciate Ligament in Beagle Dogs.

Vet Comp Orthop Traumatol 2021 May 12. Epub 2021 May 12.

Department of Clinical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, New York, United States.

Objective:  The aim of this study was to investigate functional anatomy of the craniolateral bundle (CrLB) and caudomedial bundle (CdMB) of caudal cruciate ligament in normal Beagles.

Materials And Methods:  Twelve stifle joints of 12 Beagle cadavers that were euthanatized for reasons other than musculoskeletal disease were used. Anatomical characteristics of femoral and tibial attachment regions of the caudal cruciate ligament were described, and location and area of the footprints of the CrLB and CdMB were objectively determined. Read More

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Homeopathic Treatment in Patients with Polycystic Ovarian Syndrome: A Case Series.

Homeopathy 2021 May 12. Epub 2021 May 12.

Dr. Anjali Chatterjee Regional Research Institute for Homoeopathy, Kolkata, West Bengal. Under CCRH, Ministry of AYUSH, Govt. of India, Kolkata, West Bengal, India.

Background And Objectives:  Polycystic ovary syndrome (PCOS) is a common heterogeneous endocrine disorder in women of reproductive age. It is characterized by various clinical presentations such as ovulatory dysfunction, polycystic ovaries, and hyperandrogenism. Considering the side effects associated with conventional treatment and the patients who fail to respond to these measures, there is a demand for a complementary therapy that would alleviate symptoms of PCOS without side effects. Read More

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Bigu-Style Fasting Affects Metabolic Health by Modulating Taurine, Glucose, and Cholesterol Homeostasis in Healthy Young Adults.

J Nutr 2021 May 12. Epub 2021 May 12.

Central Laboratory, Renmin Hospital of Wuhan University, Wuhan, China.

Background: Dynamic orchestration of metabolic pathways during continuous fasting remains unclear.

Objective: We investigated the physiological effects of Bigu-style fasting and underlying metabolic reprogramming in healthy adults.

Methods: We conducted a 5-d Bigu trial in 43 healthy subjects [age 23. Read More

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Assessing Agreement between Frequency-Specific Chirp Auditory Steady-State Response and Pure Tone Audiometry in Adults by Intraclass Correlation Coefficient.

ORL J Otorhinolaryngol Relat Spec 2021 May 12:1-9. Epub 2021 May 12.

Department of Otorhinolaryngology Head and Neck Surgery, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, China.

Introduction: Chirp auditory steady-state response (ASSR) can be used to assess frequency-specific hearing thresholds. However, its reliability has not been confirmed yet. The purpose of this proposed study is to analyze the agreement of thresholds measured by chirp-ASSR and pure tone audiometry (PTA) to investigate the value of chirp-ASSR in hearing threshold evaluation. Read More

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Non-Bullous Pemphigoid: A Single-Center Retrospective Study.

Dermatology 2021 May 12:1-7. Epub 2021 May 12.

Section of Dermatology and Venereology, Department of Medicine, University of Verona, Verona, Italy.

Introduction: Bullous pemphigoid (BP) is an autoimmune disease that typically presents with blisters, but sometimes early lesions may be eczematous, maculopapular, or urticarial. The aim of the present study was to highlight possible differences between typical bullous and non-bullous pemphigoid (NBP) and compare results with the literature. Material & methods: Patients receiving a diagnosis of BP between January 2000 and December 2019 were analyzed. Read More

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Segmenting lung lesions of COVID-19 from CT images via pyramid pooling improved Unet.

Biomed Phys Eng Express 2021 May 12. Epub 2021 May 12.

Chongqing University, No.174 Shazhengjie, Chongqing, Sichuan, 400044, CHINA.

Segmenting lesion regions of Coronavirus Disease 2019 (COVID-19) from computed tomography (CT) images is a challenge owing to COVID-19 lesions characterized by high variation, low contrast between infection lesions and around normal tissues, and blurred boundaries of infections. Moreover, a shortage of available CT dataset hinders deep learning techniques applying to tackling COVID-19. To address these issues, we propose a deep learning-based approach known as PPM-Unet to segmenting COVID-19 lesions from CT images. Read More

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Glutathione S-transferases and their implications in the lung diseases asthma and chronic obstructive pulmonary disease: Early life susceptibility?

Redox Biol 2021 May 2;43:101995. Epub 2021 May 2.

Department of Respiratory Medicine, Maastricht University Medical Center+, Maastricht, the Netherlands. Electronic address:

Our lungs are exposed daily to airborne pollutants, particulate matter, pathogens as well as lung allergens and irritants. Exposure to these substances can lead to inflammatory responses and may induce endogenous oxidant production, which can cause chronic inflammation, tissue damage and remodeling. Notably, the development of asthma and Chronic Obstructive Pulmonary Disease (COPD) is linked to the aforementioned irritants. Read More

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A novel approach to diagnose sleep apnea using enhanced frequency extraction network.

Comput Methods Programs Biomed 2021 Apr 28;206:106119. Epub 2021 Apr 28.

Otorhinolaryngology Hospital, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China.

Sleep apnea-hypopnea syndrome (SAHS), as a widespread respiratory sleep disorder, if left untreated, can lead to a series of pathological changes. By using Polysomnography (PSG), traditional SAHS diagnosis tends to be complex and costly. Nasal airflow (NA) is the most direct reflection of the severity of SAHS. Read More

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The effect of cervical intervertebral disc degeneration on the motion path of instantaneous center of rotation at degenerated and adjacent segments: A finite element analysis.

Comput Biol Med 2021 May 4;134:104426. Epub 2021 May 4.

Department of Orthopaedic Surgery, Beijing Tiantan Hospital, Capital Medical University, No.119, South 4th Ring West Road, Fengtai District, Beijing, 100070, China; National Clinical Research Center for Orthopedics, Sports Medicine & Rehabilitation, Beijing, 100853, China. Electronic address:

Background: The motion path of instantaneous center of rotation (ICR) is a crucial kinematic parameter to dynamically characterize cervical spine intervertebral patterns of motion; however, few studies have evaluated the effect of cervical disc degeneration (CDD) on ICR motion path. The purpose of this study was to investigate the effect of CDD on the ICR motion path of degenerated and adjacent segments.

Method: A validated nonlinear three-dimensional finite element (FE) model of a healthy adult cervical spine was used. Read More

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Association of obstructive sleep apnea with cognitive decline and age among non-demented older adults.

Neurosci Lett 2021 May 9:135955. Epub 2021 May 9.

Department of Neurology, Taizhou Hospital of Zhejiang Province Affiliated to Wenzhou Medical University, China.

To investigate whether obstructive sleep apnea (OSA) status affects the relationship between cognitive decline and age among non-demented elderly people. A total of 1422 participants (493 normal cognition and 929 amnestic mild cognitive impairment) were obtained from the Alzheimer's Disease Neuroimaging Initiative (ADNI) cohort. Based on self-reported medical history of OSA, participants were categorized into two groups (OSA- and OSA +). Read More

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The association between transthoracic echocardiogram parameters and white matter hyperintensities.

Clin Neurol Neurosurg 2021 May 4;206:106672. Epub 2021 May 4.

Department of Neurology, University of Utah, Salt Lake City, UT 84132, USA. Electronic address:

Objective: Identify abnormal cardiac chamber size and hemodynamic parameters on transthoracic echocardiogram (TTE) as predictors of advancing cerebral small vessel disease (CSVD) on brain magnetic resonance imaging (MRI).

Materials And Methods: A retrospective chart review of adults with a brain MRI and a 2-dimensional TTE was performed. WMH measured by the Fazekas score served as the primary outcome. Read More

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Single-Cell Technologies and Analyses in Hematopoiesis and Hematological Malignancies.

Exp Hematol 2021 May 9. Epub 2021 May 9.

Cancer Epigenetics Group, Josep Carreras Leukaemia Research Institute (IJC), Badalona, Barcelona, Catalonia, Spain; Centro de Investigación Biomédica en Red Cancer (CIBERONC), Madrid, Spain.; Institucio Catalana de Recerca i Estudis Avançats (ICREA), Barcelona, Catalonia, Spain; Physiological Sciences Department, School of Medicine and Health Sciences, University of Barcelona (UB), Barcelona, Catalonia, Spain. Electronic address:

In recent years, single-cell technologies have emerged as breakthrough techniques that enable the characterization of hematopoietic cell populations of normal and malignant tissue samples and will be combined in the near future with bulk technologies, currently used in clinical practice, in order to improve diagnosis, prognosis and search for novel molecular targets. These single-cell methods have the advantage of not masking cell-to-cell variation features and involve the study of genetic, epigenetic, transcriptional and proteomic landscapes from a single-cell perspective. Latest advances in this field have enabled the development of novel strategies that significantly increase both sensitivity and high-throughput. Read More

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The serum and tissue expression of vascular endothelial growth factor-in recurrent respiratory papillomatosis.

Int J Pediatr Otorhinolaryngol 2021 May 5;146:110737. Epub 2021 May 5.

Department of Otolaryngology, Head & Neck Surgery, All India Institute of Medical Sciences, New Delhi, India.

Objective: Recurrent respiratory papillomatosis (RRP) is known for its recurrent relapse despite various surgical and non-surgical treatments. Vascular Endothelial growth factor (VEGF) receptor expression on tissue is reported to be raised in RRP, and anti-VEGF targeted treatment is being explored to decrease recurrences. This study aims to identify the patients most suitable for systemic anti-VEGF therapy. Read More

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Slow-Cycling (Dormant) Cancer Cells in Therapy Resistance, Cancer Relapse and Metastasis.

Semin Cancer Biol 2021 May 9. Epub 2021 May 9.

Department of Pharmacology & Therapeutics, Roswell Park Comprehensive Cancer Center, Buffalo, NY, 14263, USA; Department of Epigenetics and Molecular Carcinogenesis, University of Texas MD Anderson Cancer Center, Smithville, TX, 78957, USA. Electronic address:

It is increasingly appreciated that cancer cell heterogeneity and plasticity constitute major barriers to effective clinical treatments and long-term therapeutic efficacy. Research in the past two decades suggest that virtually all treatment-naive human cancers harbor subsets of cancer cells that possess many of the cardinal features of normal stem cells. Such stem-like cancer cells, operationally defined as cancer stem cells (CSCs), are frequently quiescent and dynamically change and evolve during tumor progression and therapeutic interventions. Read More

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Targeting SUMOylation dependency in human cancer stem cells through a unique SAE2 motif revealed by chemical genomics.

Cell Chem Biol 2021 May 7. Epub 2021 May 7.

Stem Cell and Cancer Research Institute, McMaster University, Hamilton, ON L8S 4K1, Canada; Department of Biochemistry and Biomedical Sciences, McMaster University, Hamilton, ON L8S 4K1, Canada. Electronic address:

Natural products (NPs) encompass a rich source of bioactive chemical entities. Here, we used human cancer stem cells (CSCs) in a chemical genomics campaign with NP chemical space to interrogate extracts from diverse strains of actinomycete for anti-cancer properties. We identified a compound (McM25044) capable of selectively inhibiting human CSC function versus normal stem cell counterparts. Read More

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Impaired glucose-1,6-biphosphate production due to bi-allelic PGM2L1 mutations is associated with a neurodevelopmental disorder.

Am J Hum Genet 2021 May 5. Epub 2021 May 5.

de Duve Institute, UCLouvain, 1200 Brussels, Belgium. Electronic address:

We describe a genetic syndrome due to PGM2L1 deficiency. PGM2 and PGM2L1 make hexose-bisphosphates, like glucose-1,6-bisphosphate, which are indispensable cofactors for sugar phosphomutases. These enzymes form the hexose-1-phosphates crucial for NDP-sugars synthesis and ensuing glycosylation reactions. Read More

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Social isolation impairs the prefrontal-nucleus accumbens circuit subserving social recognition in mice.

Cell Rep 2021 May;35(6):109104

Department of Physiology, Seoul National University College of Medicine, Seoul 03080, Korea; Department of Biomedical Sciences, Seoul National University College of Medicine, Seoul 03080, Korea; Neuroscience Research Institute, Seoul National University College of Medicine, Seoul 03080, Korea. Electronic address:

Although medial prefrontal cortex (mPFC) is known to play important roles in social behaviors, how early social experiences affect the mPFC and its subcortical circuit remains unclear. We report that mice singly housed (SH) for 8 weeks after weaning show a social recognition deficit, even after 4 weeks of resocialization. In SH mice, prefrontal infralimbic (IL) neurons projecting to the shell region of nucleus accumbens (NAcSh) show decreased excitability compared with group-housed (GH) mice. Read More

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The f subunit of human ATP synthase is essential for normal mitochondrial morphology and permeability transition.

Cell Rep 2021 May;35(6):109111

Department of Biomedical Sciences, University of Padova, Padova 35121, Italy; Consiglio Nazionale delle Ricerche Institute of Neuroscience, Padova 35121, Italy; Department of Biomedical and Neuromotor Sciences, University of Bologna, Bologna 40126, Italy. Electronic address:

The f subunit is localized at the base of the ATP synthase peripheral stalk. Its function in the human enzyme is poorly characterized. Because full disruption of its ATP5J2 gene with the CRISPR-Cas9 strategy in the HAP1 human model has been shown to cause alterations in the amounts of other ATP synthase subunits, here we investigated the role of the f subunit in HeLa cells by regulating its levels through RNA interference. Read More

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A Difficult Diagnosis of Plasmodium ovale Malaria.

Acta Med Port 2021 May 12. Epub 2021 May 12.

Global Health and Tropical Medicine. Instituto de Higiene e Medicina Tropical. Universidade NOVA de Lisboa. Lisboa. Infectious and Tropical Diseases Department. Hospital Egas Moniz. Centro Hospitalar Lisboa Ocidental. Lisboa. NOVA Medical School. Universidade NOVA de Lisboa. Lisboa. Portugal.

Malaria is a major cause of suffering, disease, and death worldwide and is considered the most important of all human parasitic diseases. Malaria is still endemic in most tropical and sub-tropical areas and globalization has contributed to an increase of imported cases around the world. We report a Plasmodium ovale infection in a traveler with recent return from a long land trip across West Africa. Read More

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Fatal cerebral hemorrhage in a patient with thrombotic thrombocytopenic purpura with a normal platelet count during treatment with caplacizumab.

Platelets 2021 May 12:1-2. Epub 2021 May 12.

Department of Internal Medicine, Meander Medical Centre, Amersfoort, The Netherlands.

Acquired thrombotic thrombocytopenic purpura (aTTP) is a thrombotic microangiopathy with a severe mortality and morbidity. Caplacizumab has recently been approved in the Netherlands as a new therapeutic option in patients with life-threatening organ failure due to aTTP. We describe the case of a 50 year old patient with aTTP who was referred to our hospital for treatment with caplacizumab. Read More

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Tumor necrosis factor alpha expression is increased in maternal microvascular endothelial cells in preeclampsia.

Hypertens Pregnancy 2021 May 12:1-9. Epub 2021 May 12.

Department of Obstetrics and Gynecology, Sunnybrook Health Sciences Centre, University of Toronto, Toronto, Ontario, Canada.

The aim of this study was to explore the expression and effects of tumor necrosis factor alpha (TNFα) in maternal endothelial cells in preeclampsia (PE). Expression levels in primary microvascular endothelial cells (MVEC) isolated from patients with severe preeclampsia (PE) and normal pregnancies were determined by RT-qPCR with or without treatment of TNFα and inhibitors for downstream signaling. PE MVEC exhibited increased basal TNFα expression. Read More

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Understanding the Structural Pathways for Lipid Nanodisc Formation: How Styrene Maleic Acid Copolymers Induce Membrane Fracture and Disc Formation.

Langmuir 2021 May 12. Epub 2021 May 12.

Department of Chemistry, University of Oslo, Sem Sælandsvei 26, 0371 Oslo, Norway.

Lipid nanodiscs formed by mixtures of styrene maleic acid (SMA) copolymers and lipid membranes are important tools for studying membrane proteins in many biotechnological applications. However, molecular interactions leading up to their formation are not well understood. Here, we elucidate the nanodisc formation pathways for SMA/lipid vesicle mixtures using small-angle X-ray scattering (SAXS) that allows detailed in situ nanostructural information. Read More

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Direct Immunodetection of Global A-to-I RNA Editing Activity with a Chemiluminescent Bioassay.

Angew Chem Int Ed Engl 2021 May 12. Epub 2021 May 12.

Emory University, Department of Chemistry, 1515 Dickey Dr., 30322, Atlanta, UNITED STATES.

Adenosine-to-inosine (A-to-I) editing is a conserved RNA modification that contributes to immune response and overall cellular function. RNA editing patterns can vary significantly between different cell and tissue types, and hyperactive A-to-I signatures are indicative of several diseases, including cancer and autoimmune disorders. Because of the importance of these differences, there is significant need for efficient methods to measure overall A-to-I editing levels in cellular RNA. Read More

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Auditory evoked potentials: Differences by sex, race, and menstrual cycle and correlations with common psychoacoustical tasks.

PLoS One 2021 12;16(5):e0251363. Epub 2021 May 12.

Department of Speech, Language, and Hearing Sciences, University of Texas, Austin, Texas, United States of America.

Auditory brainstem responses (ABRs) and auditory middle-latency responses (AMLRs) to a click stimulus were measured in about 100 subjects. Of interest were the sex differences in those auditory evoked potentials (AEPs), the correlations between the various AEP measures, and the correlations between the AEP measures and measures of otoacoustic emissions (OAEs) and behavioral performance also measured on the same subjects. Also of interest was how the menstrual cycle affected the various AEP measures. Read More

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Factors determining cognitive, motor and language scores in low birth weight infants from North India.

PLoS One 2021 12;16(5):e0251387. Epub 2021 May 12.

Department of Research, Innlandet Hospital Trust, Lillehammer, Norway.

Background: Children born with low birth weight (LBW) tend to have lower neurodevelopmental scores compared to term normal birth weight children. It is important to determine factors that influence neurodevelopment in these low birth weight children especially in the first 2-3 years of life that represents a period of substantial brain development.

Methods: This secondary data analysis was conducted using data from LBW infants enrolled soon after birth in an individually randomized controlled trial (RCT) and followed up till end of 1st year. Read More

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Circulating adiponectin mediates the association between omentin gene polymorphism and cardiometabolic health in Asian Indians.

PLoS One 2021 12;16(5):e0238555. Epub 2021 May 12.

Department of Molecular Genetics, Madras Diabetes Research Foundation, Chennai, India.

Background: Plasma omentin levels have been shown to be associated with circulating adiponectin concentrations and cardiometabolic disease-related outcomes. In this study, we aim to examine the association of omentin gene polymorphism with serum adiponectin levels and cardiometabolic health status using a genetic approach, and investigate whether these associations are modified by lifestyle factors.

Methods: The study included 945 normal glucose tolerant and 941 unrelated individuals with type 2 diabetes randomly selected from the Chennai Urban Rural Epidemiology Study (CURES), in southern India. Read More

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Molecular dynamics shows complex interplay and long-range effects of post-translational modifications in yeast protein interactions.

PLoS Comput Biol 2021 May 12;17(5):e1008988. Epub 2021 May 12.

KU Leuven, Centre of Microbial and Plant Genetics, Leuven, Belgium.

Post-translational modifications (PTMs) play a vital, yet often overlooked role in the living cells through modulation of protein properties, such as localization and affinity towards their interactors, thereby enabling quick adaptation to changing environmental conditions. We have previously benchmarked a computational framework for the prediction of PTMs' effects on the stability of protein-protein interactions, which has molecular dynamics simulations followed by free energy calculations at its core. In the present work, we apply this framework to publicly available data on Saccharomyces cerevisiae protein structures and PTM sites, identified in both normal and stress conditions. Read More

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Decreased HLF Expression Predicts Poor Survival in Lung Adenocarcinoma.

Med Sci Monit 2021 May 12;27:e929333. Epub 2021 May 12.

Department of Respiratory Medicine, Shanghai University of Medicine and Health Sciences Affiliated Zhoupu Hospital, Shanghai, China (mainland).

BACKGROUND Lung adenocarcinoma (LUAD) is a type of non-small cell carcinoma. Its pathogenesis is being explored and there is no cure for the disease. MATERIAL AND METHODS The Gene Expression Omnibus (GEO) was searched to obtain data on expression of messenger RNA. Read More

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