70 results match your criteria nidulans suitable

Transcriptomic, Protein-DNA Interaction, and Metabolomic Studies of VosA, VelB, and WetA in Aspergillus nidulans Asexual Spores.

mBio 2021 02 9;12(1). Epub 2021 Feb 9.

School of Food Science and Biotechnology, Kyungpook National University, Daegu, Republic of Korea

In filamentous fungi, asexual development involves cellular differentiation and metabolic remodeling leading to the formation of intact asexual spores. The development of asexual spores (conidia) in is precisely coordinated by multiple transcription factors (TFs), including VosA, VelB, and WetA. Notably, these three TFs are essential for the structural and metabolic integrity, i. Read More

View Article and Full-Text PDF
February 2021

Fungal Dioxygenase AsqJ Is Promiscuous and Bimodal: Substrate-Directed Formation of Quinolones versus Quinazolinones.

Angew Chem Int Ed Engl 2021 04 25;60(15):8297-8302. Epub 2021 Feb 25.

Department of Chemistry and Food Chemistry, Chair of Technical Biochemistry, Technical University of Dresden, Bergstraße 66, 01069, Dresden, Germany.

Previous studies showed that the Fe /α-ketoglutarate dependent dioxygenase AsqJ induces a skeletal rearrangement in viridicatin biosynthesis in Aspergillus nidulans, generating a quinolone scaffold from benzo[1,4]diazepine-2,5-dione substrates. We report that AsqJ catalyzes an additional, entirely different reaction, simply by a change in substituent in the benzodiazepinedione substrate. This new mechanism is established by substrate screening, application of functional probes, and computational analysis. Read More

View Article and Full-Text PDF

Ocular Inserts of Voriconazole-Loaded Proniosomal Gels: Formulation, Evaluation and Microbiological Studies.

Int J Nanomedicine 2020 12;15:7825-7840. Epub 2020 Oct 12.

Department of Pharmaceutics, Faculty of Pharmacy, Mansoura University, Mansoura, Egypt.

Background: Voriconazole (VRC) is a triazole broad spectrum antifungal drug, used in the management of versatile fungal infections, particularly fungal keratitis. The obligatory use of niosomal delivery of VRC may reduce the frequency of dosing intervals resulting from its short biological half time and consequently improve patient compliance.

Methods: VRC loaded proniosomes (VRC-PNs) were set by the coacervation technique and completely characterized. Read More

View Article and Full-Text PDF
November 2020

Screening of five marine-derived fungal strains for their potential to produce oxidases with laccase activities suitable for biotechnological applications.

BMC Biotechnol 2020 05 12;20(1):27. Epub 2020 May 12.

Ecole Nationale d'Ingénieurs de Sfax, Laboratoire de Biochimie et de Génie enzymatique des lipases, Université de Sfax, Sfax, Tunisie.

Background: Environmental pollution is one of the major problems that the world is facing today. Several approaches have been taken, from physical and chemical methods to biotechnological strategies (e.g. Read More

View Article and Full-Text PDF

Genomic Locus of a Penicillium crustosum Pigment as an Integration Site for Secondary Metabolite Gene Expression.

ACS Chem Biol 2019 06 12;14(6):1227-1234. Epub 2019 Jun 12.

Institut für Pharmazeutische Biologie und Biotechnologie , Philipps-Universität Marburg , Robert-Koch-Straße 4 , 35037 Marburg , Germany.

Heterologous expression of secondary metabolite genes and gene clusters has been proven to be a successful strategy for identification of new natural products of cryptic or silent genes hidden in the genome sequences. It is also a useful tool to produce designed compounds by synthetic biology approaches. In this study, we demonstrate the potential usage of the gene locus pcr4401 in the fast-growing filamentous fungus Penicillium crustosum as an integration site for heterologous gene expression. Read More

View Article and Full-Text PDF

Duplications and losses of genes encoding known elements of the stress defence system of the Aspergilli contribute to the evolution of these filamentous fungi but do not directly influence their environmental stress tolerance.

Stud Mycol 2018 Sep 11;91:23-36. Epub 2018 Oct 11.

Department of Biotechnology and Microbiology, Faculty of Science and Technology, University of Debrecen, Egyetem tér 1, H-4032, Debrecen, Hungary.

The contribution of stress protein duplication and deletion events to the evolution of the Aspergilli was studied. We performed a large-scale homology analysis of stress proteins and generated and analysed three stress defence system models based on , and . Although both yeast-based and -based models were suitable to trace evolutionary changes, the -based model performed better in mapping stress protein radiations. Read More

View Article and Full-Text PDF
September 2018

An efficient Agrobacterium-mediated transformation method for aflatoxin generation fungus Aspergillus flavus.

J Microbiol 2018 May 2;56(5):356-364. Epub 2018 May 2.

School of Life Sciences, Anhui Agricultural University, Hefei, 230036, P. R. China.

Aspergillus flavus often invade many important corps and produce harmful aflatoxins both in preharvest and during storage stages. The regulation mechanism of aflatoxin biosynthesis in this fungus has not been well explored mainly due to the lack of an efficient transformation method for constructing a genome-wide gene mutant library. This challenge was resolved in this study, where a reliable and efficient Agrobacterium tumefaciens-mediated transformation (ATMT) protocol for A. Read More

View Article and Full-Text PDF

Biosynthesis of cobalt oxide nanoparticles using endophytic fungus Aspergillus nidulans.

J Environ Manage 2018 Jul 27;218:442-450. Epub 2018 Apr 27.

Department of Chemical Engineering, National Institute of Technology Karnataka, Surathkal, Mangaluru, 575 025, India. Electronic address:

Metallic oxide nanoparticles have profound applications in electrochemical devices, supercapacitors, biosensors and batteries. Though four fungi were isolated from Nothapodytes foetida, Aspergillus nidulans was found to be suitable for synthesis of cobalt oxide nanoparticles, as it has proficient tolerance towards metal under study. The broth containing precursor solution and organism Aspergillus nidulans had changed from pink to orange indicating the formation of nanoparticles. Read More

View Article and Full-Text PDF

Acetamidase as a dominant recyclable marker for Komagataella phaffii strain engineering.

Appl Microbiol Biotechnol 2018 Mar 12;102(6):2753-2761. Epub 2018 Feb 12.

Instituto de Ciências Biológicas, Departamento de Biologia Celular, Universidade de Brasília, Brasília, DF, 70910-900, Brazil.

We have investigated the use of the gene coding for acetamidase (amdS) as a recyclable dominant marker for the methylotrophic yeast Komagataella phaffii in order to broaden its genetic toolbox. First, the endogenous constitutive AMD2 gene (a putative acetamidase) was deleted generating strain LA1. A cassette (amdSloxP) was constructed bearing a codon-optimized version of the Aspergillus nidulans amdS gene flanked by loxP sites for marker excision with Cre recombinase. Read More

View Article and Full-Text PDF

Biological evaluation of 3-hydroxybenzyl alcohol, an extrolite produced by Aspergillus nidulans strain KZR-132.

J Appl Microbiol 2017 Jun 8;122(6):1518-1528. Epub 2017 May 8.

Nuclear Magnetic Resonance Centre, CSIR-Indian Institute of Chemical Technology, Tarnaka, Hyderabad, India.

Aims: The aim of the study was to purify and characterize a bioactive compound from Aspergillus nidulans strain KZR-132 and its biological evaluation.

Methods And Results: A bioactive extolite was purified from A. nidulans strain KZR-132, and its chemical structure was elucidated as 3-hydroxylbenzyl alcohol (3-HBA) based on H and C NMR, FT-IR and mass spectroscopic analysis. Read More

View Article and Full-Text PDF

HisB as novel selection marker for gene targeting approaches in Aspergillus niger.

BMC Microbiol 2017 03 8;17(1):57. Epub 2017 Mar 8.

Institute of Biotechnology, Department of Applied and Molecular Microbiology, Technische Universität Berlin, Gustav-Meyer-Allee 25, 13355, Berlin, Germany.

Background: For Aspergillus niger, a broad set of auxotrophic and dominant resistance markers is available. However, only few offer targeted modification of a gene of interest into or at a genomic locus of choice, which hampers functional genomics studies. We thus aimed to extend the available set by generating a histidine auxotrophic strain with a characterized hisB locus for targeted gene integration and deletion in A. Read More

View Article and Full-Text PDF

Evaluation of Biosynthetic Pathway and Engineered Biosynthesis of Alkaloids.

Molecules 2016 Aug 18;21(8). Epub 2016 Aug 18.

Department of Pharmaceutical Sciences, University of Shizuoka, Shizuoka 422-8526, Japan.

Varieties of alkaloids are known to be produced by various organisms, including bacteria, fungi and plants, as secondary metabolites that exhibit useful bioactivities. However, understanding of how those metabolites are biosynthesized still remains limited, because most of these compounds are isolated from plants and at a trace level of production. In this review, we focus on recent efforts in identifying the genes responsible for the biosynthesis of those nitrogen-containing natural products and elucidating the mechanisms involved in the biosynthetic processes. Read More

View Article and Full-Text PDF

Selective natural induction of laccases in Pleurotus sajor-caju, suitable for application at a biofuel cell cathode at neutral pH.

Bioresour Technol 2016 Oct 1;218:455-62. Epub 2016 Jul 1.

Institute for Applied Biosciences - Department of Microbiology, Karlsruhe Institute of Technology (KIT), D-76187 Karlsruhe, Germany. Electronic address:

Laccases are multicopper oxidoreductases with broad substrate specificity and are applied in biofuel cells at the cathode to improve its oxygen reduction performance. However, the production of laccases by e.g. Read More

View Article and Full-Text PDF
October 2016

Co-cultivation of Aspergillus nidulans Recombinant Strains Produces an Enzymatic Cocktail as Alternative to Alkaline Sugarcane Bagasse Pretreatment.

Front Microbiol 2016 28;7:583. Epub 2016 Apr 28.

Department of Biology, Faculty of Philosophy, Sciences and Letters of Ribeirão Preto, University of São Paulo São Paulo, Brazil.

Plant materials represent a strategic energy source because they can give rise to sustainable biofuels through the fermentation of their carbohydrates. A clear example of a plant-derived biofuel resource is the sugar cane bagasse exhibiting 60-80% of fermentable sugars in its composition. However, the current methods of plant bioconversion employ severe and harmful chemical/physical pretreatments raising biofuel cost production and environmental degradation. Read More

View Article and Full-Text PDF

Development and validation of a custom microarray for global transcriptome profiling of the fungus Aspergillus nidulans.

Curr Genet 2016 Nov 1;62(4):897-910. Epub 2016 Apr 1.

Laboratoire VEAC, Faculté des Sciences, Univ. Paris-Sud, Université Paris-Saclay, Bât. 360, Rue du Doyen André Guinier, 91405, Orsay, France.

Transcriptome profiling is a powerful tool for identifying gene networks from whole genome expression analysis in many living species. Here is described the first extensively characterized platform using Agilent microarray technology for transcriptome analysis in the filamentous fungus Aspergillus (Emericella) nidulans. We developed and validated a reliable gene expression microarray in 8 × 15 K format, with predictive and experimental data establishing its specificity and sensitivity. Read More

View Article and Full-Text PDF
November 2016

Soil fungi for mycoremediation of arsenic pollution in agriculture soils.

J Appl Microbiol 2015 Nov;119(5):1278-90

CSIR-National Botanical Research Institute, Lucknow, India.

Aims: Soil arsenic (As) contamination of food-chains and public health can be mitigated through fungal bioremediation. To enumerate culturable soil fungi, soils were collected from the As-contaminated paddy fields (3-35 mg kg(-1) ) of the middle Indo-Gangetic Plains.

Methods And Results: Total 54 fungal strains were obtained and identified at their molecular level. Read More

View Article and Full-Text PDF
November 2015

The Aspergillus nidulans bimC4 mutation provides an excellent tool for identification of kinesin-14 inhibitors.

Fungal Genet Biol 2015 Sep 24;82:51-5. Epub 2015 Jun 24.

Department of Biochemistry and Molecular Biology, The Uniformed Services University of the Health Sciences - F. Edward Hébert School of Medicine, Bethesda, MD, United States. Electronic address:

Centrosome amplification is a hallmark of many types of cancer cells, and clustering of multiple centrosomes is critical for cancer cell survival and proliferation. Human kinesin-14 HSET/KFIC1 is essential for centrosome clustering, and its inhibition leads to the specific killing of cancer cells with extra centrosomes. Since kinesin-14 motor domains are conserved evolutionarily, we conceived a strategy of obtaining kinesin-14 inhibitors using Aspergillus nidulans, based on the previous result that loss of the kinesin-14 KlpA rescues the non-viability of the bimC4 kinesin-5 mutant at 42 °C. Read More

View Article and Full-Text PDF
September 2015

An Efficient PEG/CaCl₂-Mediated Transformation Approach for the Medicinal Fungus Wolfiporia cocos.

J Microbiol Biotechnol 2015 Sep;25(9):1528-31

College of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan 430023, P.R. China.

Sclerotia of Wolfiporia cocos are of medicinal and culinary value. The genes and molecular mechanisms involved in W. cocos sclerotial formation are poorly investigated because of the lack of a suitable and reproducible transformation system for W. Read More

View Article and Full-Text PDF
September 2015

Recombinant Aspergillus β-galactosidases as a robust glycomic and biotechnological tool.

Appl Microbiol Biotechnol 2014 Apr 15;98(8):3553-67. Epub 2013 Sep 15.

Department of Chemistry, University of Natural Resources and Life Sciences, Muthgasse 18, Vienna, 1190, Austria.

Galactosidases are widespread enzymes that are used for manifold applications, including production of prebiotics, biosynthesis of different transgalactosylated products, improving lactose tolerance and in various analytical approaches. The nature of these applications often require galactosidases to be present in a purified form with clearly defined properties, including precisely determined substrate specificities, low sensitivity to inhibitors, and high efficiency and stability under distinct conditions. In this study, we present the recombinant expression and purification of two previously uncharacterized β-galactosidases from Aspergillus nidulans as well as one β-galactosidase from Aspergillus niger. Read More

View Article and Full-Text PDF

An Acidic Thermostable Recombinant Aspergillus nidulans Endoglucanase Is Active towards Distinct Agriculture Residues.

Enzyme Res 2013 10;2013:287343. Epub 2013 Jul 10.

Department of Cellular Biology, Institute of Biological Sciences, University of Brasilia, 70.910-900 Brasilia, DF, Brazil.

Aspergillus nidulans is poorly exploited as a source of enzymes for lignocellulosic residues degradation for biotechnological purposes. This work describes the A. nidulans Endoglucanase A heterologous expression in Pichia pastoris, the purification and biochemical characterization of the recombinant enzyme. Read More

View Article and Full-Text PDF

Current understanding of HOG-MAPK pathway in Aspergillus fumigatus.

Dongmei Ma Ruoyu Li

Mycopathologia 2013 Feb 18;175(1-2):13-23. Epub 2012 Nov 18.

Department of Dermatology, Peking University First Hospital, and Research Center for Medical Mycology, Peking University, No. 8 Xi-Shi-Ku St. Xicheng District, Beijing, 100034, China.

Aspergillus fumigatus is an important opportunistic fungal pathogen that causes lethal systemic invasive aspergillosis. It must be able to adapt to stress in the microenvironment during host invasion and systemic spread. The high-osmolarity glycerol (HOG) mitogen-activated protein kinase (MAPK) signaling pathway is a key element that controls adaptation to environmental stress. Read More

View Article and Full-Text PDF
February 2013

Illuminating the diversity of aromatic polyketide synthases in Aspergillus nidulans.

J Am Chem Soc 2012 May 1;134(19):8212-21. Epub 2012 May 1.

Department of Molecular Biosciences, University of Kansas, 1200 Sunnyside Avenue, Lawrence, Kansas 66045, United States.

Genome sequencing has revealed that fungi have the ability to synthesize many more natural products (NPs) than are currently known, but methods for obtaining suitable expression of NPs have been inadequate. We have developed a successful strategy that bypasses normal regulatory mechanisms. By efficient gene targeting, we have replaced, en masse, the promoters of nonreducing polyketide synthase (NR-PKS) genes, key genes in NP biosynthetic pathways, and other genes necessary for NR-PKS product formation or release. Read More

View Article and Full-Text PDF

[Application of PCR combined with reverse line blot assay in detection and identification of common pathogenic Aspergillus in fungal sinusitis].

Zhonghua Bing Li Xue Za Zhi 2012 Jan;41(1):6-10

Department of Pathology, Beijing Tongren Hospital, Capital Medical University, Beijing 100730, China.

Objective: To evaluate the feasibility of PCR/reverse line blot hybridization (RLB) assay in the detection and identification of clinical pathogens in fungal sinusitis (FS).

Methods: Twenty-six formalin-fixed and paraffin-embedded tissues and 8 fresh tissues of FS were collected from Beijing Tongren Hospital, Capital Medical University from May 2009 to February 2010. Pathological examination, fungal culture and ITS2 region sequencing were carried out. Read More

View Article and Full-Text PDF
January 2012

L-rhamnose induction of Aspergillus nidulans α-L-rhamnosidase genes is glucose repressed via a CreA-independent mechanism acting at the level of inducer uptake.

Microb Cell Fact 2012 Feb 21;11:26. Epub 2012 Feb 21.

Instituto de Agroquímica y Tecnología de Alimentos, Consejo Superior de Investigaciones Científicas, Agustín Escardino 7, Paterna, Valencia, Spain.

Background: Little is known about the structure and regulation of fungal α-L-rhamnosidase genes despite increasing interest in the biotechnological potential of the enzymes that they encode. Whilst the paradigmatic filamentous fungus Aspergillus nidulans growing on L-rhamnose produces an α-L-rhamnosidase suitable for oenological applications, at least eight genes encoding putative α-L-rhamnosidases have been found in its genome. In the current work we have identified the gene (rhaE) encoding the former activity, and characterization of its expression has revealed a novel regulatory mechanism. Read More

View Article and Full-Text PDF
February 2012

[Screening and application of phosphate-dissolving microorganism suitable for corn production].

Wei Sheng Wu Xue Bao 2010 Dec;50(12):1619-25

Chinese Academy of Agricultural Sciences Institute of Agricultural Resources and Regional Planning, Beijing 100081, China.

Objective: To obtain phosphate-dissolving strains which can be suitable for corn production.

Methods: We screened phosphate-dissolving strains from soil samples. We conducted the plate experiment and soil experiment in laboratory, pot experiment in greenhouse and field experiment to test the insoluble phosphate dissolving ability of the strains. Read More

View Article and Full-Text PDF
December 2010

Fungal development and the COP9 signalosome.

Curr Opin Microbiol 2010 Dec 9;13(6):672-6. Epub 2010 Oct 9.

Institut für Mikrobiologie und Genetik, Abteilung Molekulare Mikrobiologie und Genetik, Georg-August-Universität, Grisebachstr. 8, D-37077 Göttingen, Germany.

The conserved COP9 signalosome (CSN) multiprotein complex is located at the interface between cellular signaling, protein modification, life span and the development of multicellular organisms. CSN is required for light-controlled responses in filamentous fungi. This includes the circadian rhythm of Neurospora crassa or the repression of sexual development by light in Aspergillus nidulans. Read More

View Article and Full-Text PDF
December 2010

Antimicrobial activity of certain bacteria and fungi isolated from soil mixed with human saliva against pathogenic microbes causing dermatological diseases.

Saudi J Biol Sci 2010 Oct 23;17(4):331-339. Epub 2010 Jun 23.

Faculty of Science (Girls), Microbiology, K.A.U. Jeddah, Saudi Arabia.

Soil samples (collected from El-Madina El-Monawara, Kingdom Saudi Arabia) were mixed with human saliva, incubated in media suitable for bacterial and fungal growth and filtered. Eighteen bacterial and five fungal species were isolated and identified. The bacterial and fungal filtrates as well as the isolated species were evaluated for their antimicrobial activities against some pathogenic microbes causing dermatological diseases (, methicillin resistant (MRSA) and ). Read More

View Article and Full-Text PDF
October 2010

Expression and purification of a functional uric acid-xanthine transporter (UapA).

Protein Expr Purif 2010 Jul 11;72(1):139-46. Epub 2010 Feb 11.

Division of Molecular Biology, Imperial College London, South Kensington, London SW7 2AZ, UK.

The Nucleobase-Ascorbate Transporters (NATs) family includes carriers with fundamental functions in uptake of key cellular metabolites, such as uric acid or vitamin C. The best studied example of a NAT transporter is the uric acid-xanthine permease (UapA) from the model ascomycete Aspergillus nidulans. Detailed genetic and biochemical analyses have revealed much about the mechanism of action of this protein; however, the difficulties associated with handling eukaryotic membrane proteins have limited efforts to elucidate the precise structure-function relationships of UapA by structural analysis. Read More

View Article and Full-Text PDF

Heterologous expression of an Agaricus meleagris pyranose dehydrogenase-encoding gene in Aspergillus spp. and characterization of the recombinant enzyme.

Appl Microbiol Biotechnol 2010 Mar 4;86(2):599-606. Epub 2009 Nov 4.

Department of Food Sciences and Technology, University of Natural Resources and Applied Life Sciences (BOKU), Vienna, Austria.

Pyranose dehydrogenase (PDH) is a flavin-dependant sugar oxidoreductase found in the family Agaricaceae, basidiomycetes that degrade lignocellulose-rich forest litter, and is catalytically related to the fungal enzymes pyranose 2-oxidase and cellobiose dehydrogenase. It has broad substrate specificity and displays similar activities with most sugar constituents of lignocellulose including disaccharides and oligosaccharides, a number of (substituted) quinones, and metal ions are suitable electron acceptors rather than molecular oxygen. In contrast to pyranose 2-oxidase and cellobiose dehydrogenase, which oxidize regioselectively at C-2 and C-1, respectively, PDH is capable of oxidation on C-1 to C-4 as well as double oxidations, depending on the nature of the substrate. Read More

View Article and Full-Text PDF

Identification of novel cell wall destabilizing antifungal compounds using a conditional Aspergillus nidulans protein kinase C mutant.

J Antimicrob Chemother 2009 Oct 31;64(4):755-63. Epub 2009 Jul 31.

Department of Human Microbiology, Sackler School of Medicine, Tel-Aviv University, Tel-Aviv, Israel.

Objectives: Despite the need for novel drugs to combat fungal infections, antifungal drug discovery is currently limited by both the availability of suitable drug targets and assays to screen corresponding targets. The aim of this study was to screen a library of small chemical compounds to identify cell wall inhibitors using a conditional protein kinase C (PKC)-expressing strain of Aspergillus nidulans. This mutant is specifically susceptible to cell wall damaging compounds under PKC-repressive growth conditions. Read More

View Article and Full-Text PDF
October 2009