4,629 results match your criteria neutrophils subsequent

Group-2 Innate Lymphoid Cells Promote Hepatocellular Carcinoma Progression via CXCL2-Neutrophil Induced Immunosuppression.

Hepatology 2021 Apr 8. Epub 2021 Apr 8.

Department of Hepatobiliary and Pancreatic Surgery, the First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China.

Background & Aims: Due to their inherent characteristics, the function of group-2 innate lymphoid cells (ILC2s) varies in a context-dependent manner. ILC2s are involved in certain liver diseases; however, their involvement in hepatocellular carcinoma (HCC) is unknown. In the present study, we assessed the role of an HCC-derived ILC2 population in tumor progression. Read More

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The effect of low versus high tidal volume ventilation on inflammatory markers in animal model undergoing lung ventilation: A prospective study.

Saudi J Anaesth 2021 Jan-Mar;15(1):1-6. Epub 2021 Jan 5.

Department of Anesthesia, College of Medicine, King Saud University, Riyadh, Kingdom of Saudi Arabia.

Background And Aims: Mechanical ventilation (MV) with high tidal volume (Vt.) may induce or aggravate lung injury in critically ill patients. It might also cause an overwhelming systemic inflammation leading to acute lung injury (ALI), diffuse alveolar damage (DAD) and multiple organ failure (MOF) with subsequent high mortality. Read More

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January 2021

Investigation and assessment of neutrophil dysfunction early after severe burn injury.

Burns 2021 Feb 20. Epub 2021 Feb 20.

Department of Burns and Plastic Surgery, Affiliated Suzhou Hospital of Nanjing Medical University, Suzhou 215002, Jiangsu Province, China. Electronic address:

Background: Extensive burn injury results in a complex immune response that is associated with mortality and prognosis. Studies on acquired immune and the development of sepsis in burn patients have been reported. However, one of the main cells in innate immune, neutrophil dysfunction in the burn shock stage has not been thoroughly characterized. Read More

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February 2021

Platelet and Neutrophil Responses to Porphyromonas gingivalis in Human Whole Blood.

Mol Oral Microbiol 2021 Apr 2. Epub 2021 Apr 2.

Division of Biochemistry, Department of Basic Sciences, School of Medicine, Loma Linda University, Loma Linda, CA, 92350, USA.

Porphyromonas gingivalis is a causative agent for periodontal disease. Binding of platelets to this gram-negative anaerobe can regulate host hemostatic (thrombus forming) and immune (neutrophil interacting) responses during bacterial infection. Additionally, in response to bacterial pathogens neutrophils can release their DNA, forming highly prothrombotic neutrophil extracellular traps (NETs), which then further exaggerate platelet responses. Read More

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Modulation of Innate Immune Toxicity by Silver Nanoparticle Exposure and the Preventive Effects of Pterostilbene.

Int J Mol Sci 2021 Mar 3;22(5). Epub 2021 Mar 3.

Department of Environmental and Occupational Health, College of Medicine, National Cheng Kung University, Tainan 70101, Taiwan.

Silver nanoparticles pose a potential risk to ecosystems and living organisms due to their widespread use in various fields and subsequent gradual release into the environment. Only a few studies have investigated the effects of silver nanoparticles (AgNPs) toxicity on immunological functions. Furthermore, these toxic effects have not been fully explored. Read More

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Lignosus rhinocerotis Cooke Ryvarden ameliorates airway inflammation, mucus hypersecretion and airway hyperresponsiveness in a murine model of asthma.

PLoS One 2021 30;16(3):e0249091. Epub 2021 Mar 30.

School of Health Sciences, Universiti Sains Malaysia, Kubang Kerian, Kelantan, Malaysia.

Lignosus rhinocerotis Cooke. (L. rhinocerotis) is a medicinal mushroom traditionally used in the treatment of asthma and several other diseases by the indigenous communities in Malaysia. Read More

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Glycosaminoglycans located on neutrophils and monocytes impact on CXCL8- and CCL2-induced cell migration.

Cytokine 2021 Mar 26:155503. Epub 2021 Mar 26.

Karl-Franzens-University Graz, Institute of Pharmaceutical Sciences, Universitätsplatz 1, A-8010 Graz, Austria; Antagonis Biotherapeutics GmbH, Strasserhofweg 77a, A-8045 Graz, Austria. Electronic address:

The role of glycosaminoglycans on the surface of immune cells has so far been less studied compared to their participation in inflammatory responses as members of the endothelium and the extracellular matrix. In this study we have therefore investigated if glycosaminoglycans on immune cells act in concert with GPC receptors (i.e. Read More

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PAD4 takes charge during neutrophil activation: impact of PAD4 mediated NET formation on immune-mediated disease.

J Thromb Haemost 2021 Mar 27. Epub 2021 Mar 27.

Department of Biochemistry, Cardiovascular Research Institute Maastricht (CARIM), Maastricht University, 6200 MD, Maastricht, The Netherlands.

Peptidyl arginine deiminase 4 (PAD4) is an enzyme expressed in neutrophils that, when activated, can drive the formation of neutrophil extracellular traps (NETs). Uncontrolled activation of PAD4 and subsequent citrullination of proteins is increasingly recognized as a driver of (auto) immune diseases. Currently, our understanding of PAD4 structure-function relationships and activity control in vivo is incomplete. Read More

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The potential for Lactoferrin to reduce SARS-CoV-2 induced cytokine storm.

Int Immunopharmacol 2021 Mar 12;95:107571. Epub 2021 Mar 12.

University of Texas, Health Science Center Houston, Texas, USA.

The COVID-19 pandemic is a serious global health threat caused by severe acute respiratory syndrome of coronavirus 2 (SARS-CoV-2). Symptoms of COVID-19 are highly variable with common hyperactivity of immune responses known as a "cytokine storm". In fact, this massive release of inflammatory cytokines into in the pulmonary alveolar structure is a main cause of mortality during COVID-19 infection. Read More

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Plasma gelsolin modulates the production and fate of IL-1β-containing microparticles following high-pressure exposure and decompression.

J Appl Physiol (1985) 2021 Mar 25. Epub 2021 Mar 25.

Emergency Medicine, University of Maryland Medical Center, United States.

Plasma gelsolin (pGSN) levels fall in association with diverse inflammatory conditions. We hypothesized pGSN would decrease due to the stresses imposed by high pressure and subsequent decompression, and repletion would ameliorate injuries in a murine decompression sickness (DCS) model. Research subjects were found to exhibit a modest decrease in pGSN level while at high pressure and a profound decrease after decompression. Read More

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Immune complex-induced apoptosis and concurrent immune complex clearance are anti-inflammatory neutrophil functions.

Cell Death Dis 2021 Mar 19;12(4):296. Epub 2021 Mar 19.

The Centre for Inflammation Research, Institute for Regeneration and Repair, The University of Edinburgh, Edinburgh, UK.

Persistent neutrophilic inflammation drives host damage in autoimmune diseases that are characterized by abundant immune complexes. Insoluble immune complexes (iICs) potently activate pro-inflammatory neutrophil effector functions. We and others have shown that iICs also promote resolution of inflammation via stimulation of neutrophil apoptosis. Read More

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The Impact of Neutrophil Recruitment to the Skin on the Pathology Induced by Infection.

Front Immunol 2021 1;12:649348. Epub 2021 Mar 1.

Department of Biochemistry, WHO Collaborative Centre for Research and Training in Immunology, University of Lausanne, Lausanne, Switzerland.

(.) are obligate intracellular protozoan parasites that cause the leishmaniases, a spectrum of neglected infectious vector-borne diseases with a broad range of clinical manifestations ranging from local cutaneous, to visceral forms of the diseases. The parasites are deposited in the mammalian skin during the blood meal of an infected female phlebotomine sand fly. Read More

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Fluorodeoxyglucose Positron Emission Tomography/Computed Tomography Imaging Predicts Vein Wall Scarring and Statin Benefit in Murine Venous Thrombosis.

Circ Cardiovasc Imaging 2021 Mar 16:CIRCIMAGING120011898. Epub 2021 Mar 16.

Cardiology Division, Cardiovascular Research Center, Massachusetts General Hospital, Harvard Medical School, Boston, MA (C.W.K., G.Q., F.A.J.).

Background: The postthrombotic syndrome is a common, often morbid sequela of venous thrombosis (VT) that arises from thrombus persistence and inflammatory scarring of juxtaposed vein walls and valves. Noninvasive F-fluorodeoxyglucose (FDG) positron emission tomography/computed tomography (PET/CT) imaging can measure neutrophil inflammation in VT. Here, we hypothesized (1) early fluorodeoxyglucose positron emission tomography/computed tomography (FDG-PET/CT) VT inflammation can predict subsequent vein wall scarring (VWS) and (2) statin therapy can reduce FDG-PET VT inflammation and subsequent VWS. Read More

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Leptin Receptor q223r Polymorphism Influences Infection-Induced Neutrophil CXCR2 Expression in an Interleukin-1β Dependent Manner.

Front Cell Infect Microbiol 2021 25;11:619192. Epub 2021 Feb 25.

Division of Infectious Diseases, Department of Internal Medicine, University of Cincinnati College of Medicine, Cincinnati, OH, United States.

Neutrophils are key first-responders in the innate immune response to infection (CDI) and play a central role in disease pathogenesis. Studies have clearly shown that tissue neutrophil numbers need to be tightly regulated for optimal CDI outcomes: while excessive colonic neutrophilia is associated with severe CDI, neutrophil depletion also results in worse outcomes. However, the biological mechanisms that control CDI-induced neutrophilia remain poorly defined. Read More

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February 2021

Appreciating the First Line of the Human Innate Immune Defense: A Strategy to Model and Alleviate the Neutrophil Elastase-Mediated Attack toward Bioactivated Biomaterials.

Small 2021 Apr 9;17(13):e2007551. Epub 2021 Mar 9.

Department of Functional Materials in Medicine and Dentistry at the Institute of Functional Materials and Biofabrication (IFB), University of Würzburg and KeyLab Polymers for Medicine of the Bavarian Polymer Institute (BPI), Pleicherwall 2, Würzburg, 97070, Germany.

Biointerface engineering is a wide-spread strategy to improve the healing process and subsequent tissue integration of biomaterials. Especially the integration of specific peptides is one promising strategy to promote the regenerative capacity of implants and 3D scaffolds. In vivo, these tailored interfaces are, however, first confronted with the innate immune response. Read More

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Platelet TLR4-ERK5 Axis Facilitates NET-Mediated Capturing of Circulating Tumor Cells and Distant Metastasis after Surgical Stress.

Cancer Res 2021 Mar 9. Epub 2021 Mar 9.

Department of Surgery, The Ohio State University Medical Center, Columbus, Ohio.

Surgical removal of malignant tumors is a mainstay in controlling most solid cancers. However, surgical insult also increases the risk of tumor recurrence and metastasis. Tissue trauma activates the innate immune system locally and systemically, mounting an inflammatory response. Read More

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Transient Neutropenia in Immunocompetent Infants with Respiratory Syncytial Virus Infection.

Viruses 2021 02 15;13(2). Epub 2021 Feb 15.

Department of Pediatrics, National Hospital Organization Beppu Medical Center, 1473 Oaza-Uchikamado, Beppu, Oita 874-0011, Japan.

The incidence of neutropenia and the association between neutropenia and severity of respiratory symptoms among infants with respiratory syncytial virus (RSV) infections remain to be elucidated. This single-center, retrospective study included immunocompetent infants (<10 months old) with laboratory-confirmed RSV infection admitted to our center between January 2012 and December 2019. Incidence of neutropenia (<1. Read More

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February 2021

PLCε1 mediates one-lung ventilation injury by regulating the p38/RhoA/NFκB activation loop.

Mol Immunol 2021 May 1;133:135-145. Epub 2021 Mar 1.

Department of Anesthesiology, The First People's Hospital of Yunnan Province, The Affiliated Hospital of Kunming University of Science and Technology, No 157 Jinbi Rd, Kunming, 650032, China. Electronic address:

Background: Phospholipase C epsilon-1 (PLCε1) might be a novel and potential target in treating inflammatory conditions. In the present study, we aimed to clarify whether PLCε1 is involved in lung injury caused by one-lung ventilation (OLV) and to elucidate the potential molecular mechanism of PLCε1-mediated signaling pathway on OLV induced inflammatory response and injury.

Methods: Male Sprague-Dawley (SD) rats were divided into wide-type (PLCε1-WT) group and PLCε1-KO group, and were treated with OLV for 0. Read More

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Prognostic value of the preoperative lymphocyte-to-monocyte ratio for survival after lung cancer surgery.

BMC Pulm Med 2021 Mar 2;21(1):75. Epub 2021 Mar 2.

Department of Thoracic Surgery, Hospital Universitari de Bellvitge, Bellvitge Biomedical Research Institute (IDIBELL), Feixa Llarga s/n., 08907, L´Hospitalet de Llobregat, Barcelona, Spain.

Background: The aim of this study was to assess the effect of the lymphocyte-to-monocyte ratio (LMR), neutrophil-to-lymphocyte ratio and platelet-to-lymphocyte ratio on overall survival and disease-free survival in patients with lung cancer treated with radical surgery.

Methods: We performed a retrospective review of patients with lung cancer who prospectively underwent radical resection between 2004 and 2012. Blood samples were taken as part of the preoperative workup. Read More

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Peptidoglycan Switches Off the TLR2-Mediated Sperm Recognition and Triggers Sperm Localization in the Bovine Endometrium.

Front Immunol 2020 11;11:619408. Epub 2021 Feb 11.

Global Agromedicine Research Center (GAMRC), Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Japan.

In mammals, the uterine mucosal immune system simultaneously recognizes and reacts to most bacteria as well as allogenic sperm mainly through the Toll-like receptors (TLR)2/4 signaling pathway. Here, we characterized the impact of pathogen-derived TLR2/4 ligands (peptidoglycan (PGN)/lipopolysaccharide (LPS)) on the of sperm with the bovine endometrial epithelium. The real-time PCR analysis showed that the presence of low levels of PGN, but not LPS, blocked the sperm-induced inflammatory responses in bovine endometrial epithelial cells (BEECs) . Read More

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February 2021

Neutrophils in asthma: the good, the bad and the bacteria.

Thorax 2021 Feb 25. Epub 2021 Feb 25.

Faculty of Health and Medicine, Priority Research Centre for Healthy Lungs, The University of Newcastle, Callaghan, New South Wales, Australia

Airway inflammation plays a key role in asthma pathogenesis but is heterogeneous in nature. There has been significant scientific discovery with regard to type 2-driven, eosinophil-dominated asthma, with effective therapies ranging from inhaled corticosteroids to novel biologics. However, studies suggest that approximately 1 in 5 adults with asthma have an increased proportion of neutrophils in their airways. Read More

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February 2021

The neutrophil antimicrobial peptide cathelicidin promotes Th17 differentiation.

Nat Commun 2021 02 24;12(1):1285. Epub 2021 Feb 24.

Centre for Inflammation Research, University of Edinburgh; 47 Little France Crescent, Edinburgh, UK.

The host defence peptide cathelicidin (LL-37 in humans, mCRAMP in mice) is released from neutrophils by de-granulation, NETosis and necrotic death; it has potent anti-pathogen activity as well as being a broad immunomodulator. Here we report that cathelicidin is a powerful Th17 potentiator which enhances aryl hydrocarbon receptor (AHR) and RORγt expression, in a TGF-β1-dependent manner. In the presence of TGF-β1, cathelicidin enhanced SMAD2/3 and STAT3 phosphorylation, and profoundly suppressed IL-2 and T-bet, directing T cells away from Th1 and into a Th17 phenotype. Read More

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February 2021

Engineering of Neutrophil Membrane Camouflaging Nanoparticles Realizes Targeted Drug Delivery for Amplified Antitumor Therapy.

Int J Nanomedicine 2021 15;16:1175-1187. Epub 2021 Feb 15.

Obstetrics and Gynecology Department, Tongji Hospital of Tongji University, Shanghai, People's Republic of China.

Purpose: Although the neutrophil membrane (NM)-based nanoparticulate delivery system has exhibited rapid advances in tumor targeting stemmed from the inherited instinct, the antitumor effect requires further improvement due to inefficient cellular internalization in the absence of specific interactions between NM-coated nanoparticles and tumor cells.

Methods: Herein, we fabricated drug-paclitaxel loaded NM camouflaging nanoparticles (TNM-PN) modified with tumor necrosis factor-related apoptosis-inducing ligand (TRAIL), favorable for the cellular internalization.

Results: The results showed that TNM-PN exerted a significant cytotoxicity to tumor cells by TRAIL-mediated endocytosis and strong adhesion to inflamed endothelial cells in vitro. Read More

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Identification of Hub Genes and Immune Infiltration in Psoriasis by Bioinformatics Method.

Front Genet 2021 3;12:606065. Epub 2021 Feb 3.

Department of Dermatology, The Second Affiliated Hospital of Soochow University, Suzhou, China.

Background: Psoriasis is a chronic, prolonged, and recurrent skin inflammatory disease. However, the pathogenesis of psoriasis is not completely clear, thus we aimed to explore potential molecular basis of it.

Methods: Two datasets were downloaded from the Gene Expression Omnibus database. Read More

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February 2021

High-mobility group box protein-1 induces acute pancreatitis through activation of neutrophil extracellular trap and subsequent production of IL-1β.

Life Sci 2021 Feb 15:119231. Epub 2021 Feb 15.

Intensive Care Unit, Shengjing Hospital of China Medical University, Shenyang 110004, Liaoning, China.

Purpose: The aim of this study is to evaluate acute pancreatitis (AP)-associated NET activation mediated by a novel inflammatory mediator (high-mobility group box protein-1 [HMGB1]) and proinflammatory cytokine responses.

Methods: In this study, primary neutrophils, monocytes, and monocytic cell line Thp-1-derived macrophages were isolated and treated with HMGB1, lipopolysaccharide (LPS), adenosine triphosphate (ATP), and ATP + ATP inhibitor. The effects of HMGB1, ATP, and deoxyribonuclease (DNAse) were then examined for their in vivo effects using a newly established AP mouse model. Read More

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February 2021

Endothelial Cell Protein C Receptor Deficiency Attenuates induced Pleural Fibrosis.

Am J Respir Cell Mol Biol 2021 Apr;64(4):477-491

Department of Cellular and Molecular Biology.

is the leading cause of hospital community-acquired pneumonia. Patients with pneumococcal pneumonia may develop complicated parapneumonic effusions or empyema that can lead to pleural organization and subsequent fibrosis. The pathogenesis of pleural organization and scarification involves complex interactions between the components of the immune system, coagulation, and fibrinolysis. Read More

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Effects of storage time and temperature on highly multiparametric flow analysis of peripheral blood samples; implications for clinical trial samples.

Biosci Rep 2021 Feb;41(2)

Discipline of Pathology, Faculty of Medicine and Health, The University of Sydney, Camperdown, NSW 2050, Australia.

We sought to determine the effect of time and temperature of blood sample storage before preparation of human peripheral blood mononuclear cells (PBMCs) by Ficoll-hypaque density gradient centrifugation. Blood samples from healthy donors were stored at room temperature (RT) or refrigerated at 4°C before preparation of PBMCs. Cell yield and viability, and proportions of major cell populations within PBMCs, as determined by fluorescence flow cytometry, were assessed for both fresh and cryopreserved samples. Read More

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February 2021

5-lipoxygenase- and Glucocorticoid-dependent eosinophilia in a novel surgical model in mice.

Int Immunopharmacol 2021 Feb 12;94:107440. Epub 2021 Feb 12.

Dept. Immunology, Instituto de Microbiologia Paulo de Góes, UFRJ, Rio de Janeiro, Brazil. Electronic address:

Background: Subcutaneous implants of heat-coagulated egg white (egg white implants, EWI) induce intense local eosinophilia and prime for hyperreactivity following airway ovalbumin challenge. The roles of allergen sensitization, surgical trauma-induced glucocorticoids, and the 5-lipoxygenase (5-LO) pathway were hitherto unexplored in this model, in which quantitative recovery and large-scale purification of the eosinophils from the inflammatory site for functional and immunopharmacological studies are difficult to achieve.

Methods: We overcame this limitation by shifting the implantation site to the peritoneal cavity (EWIp), thereby enabling quantitative leukocyte retrieval. Read More

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February 2021

Antibodies for Human Immunodeficiency Virus-1 Cure Strategies.

J Infect Dis 2021 Feb;223(Supplement_1):22-31

Ragon Institute of MGH, MIT, and Harvard, Cambridge, Massachusetts, USA.

Human immunodeficiency virus (HIV) infection leads to the establishment of a long-lived latent cellular reservoir. One strategy to eliminate quiescent reservoir cells is to reactivate virus replication to induce HIV envelope glycoprotein (Env) expression on the cell surface exposing them to subsequent antibody targeting. Via the interactions between the antibody Fc domain and Fc-γ receptors (FcγRs) that are expressed on innate effector cells, such as natural killer cells, monocytes, and neutrophils, antibodies can mediate the elimination of infected cells. Read More

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February 2021

Innate Immune Cells and Hypertension: Neutrophils and Neutrophil Extracellular Traps (NETs).

Compr Physiol 2021 02 12;11(1):1575-1589. Epub 2021 Feb 12.

Program in Physiological Genomics, UT Microbiome Consortium, Center for Hypertension & Personalized Medicine, Department of Physiology & Pharmacology, University of Toledo College of Medicine and Life Sciences, Toledo, Ohio, USA.

Uncontrolled immune system activation amplifies end-organ injury in hypertension. Nonetheless, the exact mechanisms initiating this exacerbated inflammatory response, thereby contributing to further increases in blood pressure (BP), are still being revealed. While participation of lymphoid-derived immune cells has been well described in the hypertension literature, the mechanisms by which myeloid-derived innate immune cells contribute to T cell activation, and subsequent BP elevation, remains an active area of investigation. Read More

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February 2021