293 results match your criteria neutralization il-8

Cytokine Profiles and Antibody Response Associated to Choclo Orthohantavirus Infection.

Front Immunol 2021 19;12:603228. Epub 2021 Mar 19.

Department of Microbiology, Biotechnology Center, Faculty of Biological Science, Universidad de Concepción, Concepción, Chile.

Background: New World Hantaviruses (NWHs) are the etiological agent underlying hantavirus cardiopulmonary syndrome (HCPS), a severe respiratory disease with high mortality rates in humans. In Panama, infections with (CHOV) cause a much milder illness characterized by higher seroprevalence and lower mortality rates. To date, the cytokine profiles and antibody responses associated with this milder form of HCPS have not been defined. Read More

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Antibody affinity maturation and plasma IgA associate with clinical outcome in hospitalized COVID-19 patients.

Nat Commun 2021 02 22;12(1):1221. Epub 2021 Feb 22.

Division of Viral Products, Center for Biologics Evaluation and Research (CBER), FDA, Silver Spring, MD, USA.

Hospitalized COVID-19 patients often present with a large spectrum of clinical symptoms. There is a critical need to better understand the immune responses to SARS-CoV-2 that lead to either resolution or exacerbation of the clinical disease. Here, we examine longitudinal plasma samples from hospitalized COVID-19 patients with differential clinical outcome. Read More

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February 2021

Manufacturing of convalescent plasma of COVID-19 patients: Aspects of quality.

PLoS One 2020 22;15(12):e0243967. Epub 2020 Dec 22.

Institute of Clinical Chemistry and Laboratory Medicine, Transfusion Medicine, University Hospital Regensburg, Regensburg, Germany.

The ongoing coronavirus disease 2019 (COVID-19) pandemic emerged in December 2019. Convalescent plasma represents a promising COVID-19 treatment. Here, we report on the manufacturing of a plasma-based product containing antibodies specific to SARS-CoV-2 obtained from recently recovered COVID-19 patients. Read More

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January 2021

Angiopoietin-2 Promotes Inflammatory Activation in Monocytes of Systemic Sclerosis Patients.

Int J Mol Sci 2020 Dec 15;21(24). Epub 2020 Dec 15.

Center for Translational Immunology, University Medical Center Utrecht, University of Utrecht, 3508 GA Utrecht, The Netherlands.

Angiopoietin-2 (Ang-2), a ligand of the tyrosine kinase receptor Tie2, is essential for vascular development and blood vessel stability and is also involved in monocyte activation. Here, we examined the role of Ang-2 on monocyte activation in patients with systemic sclerosis (SSc). Ang-2 levels were measured in serum and skin of healthy controls (HCs) and SSc patients by ELISA and array profiling, respectively. Read More

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December 2020

Spontaneous regression of micro-metastases following primary tumor excision: a critical role for primary tumor secretome.

BMC Biol 2020 11 6;18(1):163. Epub 2020 Nov 6.

Sagol School of Neuroscience and School of Psychological Sciences, Tel Aviv University, 69978, Tel Aviv, Israel.

Background: Numerous case studies have reported spontaneous regression of recognized metastases following primary tumor excision, but underlying mechanisms are elusive. Here, we present a model of regression and latency of metastases following primary tumor excision and identify potential underlying mechanisms.

Results: Using MDA-MB-231 human breast cancer cells that express highly sensitive luciferase, we monitored early development stages of spontaneous metastases in BALB/c nu/nu mice. Read More

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November 2020

Anti-EGF antibodies as surrogate biomarkers of clinical efficacy in stage IIIB/IV non-small-cell lung cancer patients treated with an optimized CIMAvax-EGF vaccination schedule.

Oncoimmunology 2020 05 25;9(1):1762465. Epub 2020 May 25.

Clinical Research Direction, Center of Molecular Immunology, Havana, Cuba.

We previously reported that CIMAvax-EGF vaccine is safe, immunogenic and efficacious to treat advanced non-small-cell lung cancer (NSCLC) patients. A phase III trial was designed using an optimized immunization schedule. It included higher antigen dose and injections at multiple sites. Read More

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IL-1α Modulates IFN-γ-Induced Production of CXCL9/MIG during Herpes Simplex Virus Type-1 Corneal Infection.

Curr Eye Res 2021 Mar 15;46(3):309-317. Epub 2020 Sep 15.

Department of Biology, University of West Georgia , Carrollton, GA, USA.

Purpose: Investigating the modulation of neutrophil production of MIG and IP-10 during the inflammatory response to HSV-1 infection.

Materials And Methods: An model of human corneal infection by HSV-1 was used for this study. This model permits the study of cytokine production by human corneal buttons in the presence, or absence, of gradient purified human neutrophils, under conditions of HSV-1 infection. Read More

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IL-8 Released from Human Pancreatic Cancer and Tumor-Associated Stromal Cells Signals through a CXCR2-ERK1/2 Axis to Induce Muscle Atrophy.

Cancers (Basel) 2019 Nov 25;11(12). Epub 2019 Nov 25.

Department of Physical Therapy, University of Florida, Gainesville, FL 32610, USA.

Tumor-derived cytokines are known to drive the catabolism of host tissues, including skeletal muscle. However, our understanding of the specific cytokines that initiate this process remains incomplete. In the current study, we conducted multiplex analyte profiling of cytokines in conditioned medium (CM) collected from human pancreatic cancer (PC) cells, human tumor-associated stromal (TAS) cells, and their co-culture. Read More

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November 2019

LABAs and p38MAPK Inhibitors Reverse the Corticosteroid-Insensitivity of IL-8 in Airway Smooth Muscle Cells of COPD.

J Clin Med 2019 Nov 22;8(12). Epub 2019 Nov 22.

Zürcher RehaZentren Davos, 7272 Davos-Clavadel, Switzerland.

Airway inflammation in chronic obstructive pulmonary disease (COPD) is partially insensitive/resistant to inhaled corticosteroids (ICS). ICS plus bronchodilator therapy has been discussed for COPD phenotypes with frequent exacerbations and participation of corticosteroid-sensitive type 2/eosinophilic inflammation. Neutralization of non-type 2/IL-8-associated airway inflammation by reversion of its corticosteroid-resistance might be a future strategy for other phenotypes. Read More

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November 2019

Interleukin-1α leads to growth hormone deficiency in adamantinomatous craniopharyngioma by targeting pericytes: implication in pituitary fibrosis.

Metabolism 2019 12 27;101:153998. Epub 2019 Oct 27.

Department of Neurosurgery, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. Electronic address:

Background: The incidence of growth hormone deficiency (GHD) in adamantinomatous craniopharyngioma (aCP) is significantly higher than in other sellar region tumors, but the possible mechanism is still elusive. A high level of inflammatory responses is another feature of aCP. We investigated the internal connection between interleukin-1α (IL-1α) and GHD, while focusing on its biological activities in pituitary fibrosis. Read More

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December 2019

IL-8 is involved in radiation therapy resistance of esophageal squamous cell carcinoma via regulation of PCNA.

Arch Biochem Biophys 2019 11 17;676:108158. Epub 2019 Oct 17.

Oncology Department, Jiangdu People's Hospital Affiliated to Medical College of Yangzhou University, Yangzhou, 225200, China.

Esophageal squamous cell carcinoma (ESCC) is one of the prevalent and deadly cancers worldwide, especially in Eastern Asia. Our present study revealed that the expression of IL-8 was increased in radiation therapy resistance of ESCC cells. Targeted inhibition of IL-8 by its neutralization antibody (anti-IL-8) can re-sensitize ESCC cells to radiation therapy and suppress the expression of proliferating cell nuclear antigen (PCNA). Read More

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November 2019

derivatives as inhibitors of HIV-1 BaL infection.

Nat Prod Res 2021 Mar 20;35(6):1064-1069. Epub 2019 Aug 20.

School of Dental Medicine, Department Foundational Sciences, East Carolina University, Greenville, NC, USA.

The emphasis of the present study is to evaluate a natural product and the potential microbicide activity using a dual chamber infection method. extracts and fractions were screened for anti-HIV activity by measuring the virus-antibody neutralization. Plant extracts with strong antiviral activity working in nanomolar or picomolar range can be used to enhance the activity of synthetic compounds and work as anti-HIV agents. Read More

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Promotion of macrophage activation by Tie2 in the context of the inflamed synovia of rheumatoid arthritis and psoriatic arthritis patients.

Rheumatology (Oxford) 2020 02;59(2):426-438

Department of Rheumatology and Clinical Immunology, University of Utrecht, Utrecht.

Objective: To examine the role of Tie2 signalling in macrophage activation within the context of the inflammatory synovial microenvironment present in patients with RA and PsA.

Methods: Clinical responses and macrophage function were examined in wild-type and Tie2-overexpressing (Tie2-TG) mice in the K/BxN serum transfer model of arthritis. Macrophages derived from peripheral blood monocytes from healthy donors, RA and PsA patients, and RA and PsA synovial tissue explants were stimulated with TNF (10 ng/ml), angiopoietin (Ang)-1 or Ang-2 (200 ng/ml), or incubated with an anti-Ang2 neutralizing antibody. Read More

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February 2020

Immune responses against enterovirus A71 infection: Implications for vaccine success.

Rev Med Virol 2019 09 1;29(5):e2073. Epub 2019 Aug 1.

Department of Medical Microbiology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.

Enterovirus A71 (EV-A71) from the Picornaviridae family is an important emerging pathogen causing hand, foot, and mouth disease (HFMD) outbreaks worldwide. EV-A71 also caused fatal neurological complications in young children especially in Asia. On the basis of seroepidemiological studies from many Asian countries, EV-A71 infection is very common. Read More

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September 2019

Lactobacillus plantarum C88 protects against aflatoxin B-induced liver injury in mice via inhibition of NF-κB-mediated inflammatory responses and excessive apoptosis.

BMC Microbiol 2019 07 29;19(1):170. Epub 2019 Jul 29.

Institute of Agro-food Technology, Jilin Academy of Agricultural Sciences, No. 1363 Sheng-Tai Street, Changchun, Jilin Province, 130033, People's Republic of China.

Background: Probiotics play an important role in the human and animal defense against liver damage. However, the protective mechanism of Lactobacillus plantarum C88 on chronic liver injury induced by mycotoxin remains unclear.

Results: In this study, the addition of L. Read More

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The Hepatic Microenvironment and TRAIL-R2 Impact Outgrowth of Liver Metastases in Pancreatic Cancer after Surgical Resection.

Cancers (Basel) 2019 May 29;11(6). Epub 2019 May 29.

Institute for Experimental Cancer Research, Christian-Albrechts-University Kiel and University Hospital Schleswig-Holstein (UKSH) Campus Kiel, Arnold-Heller-Str. 3, Building 17, 24105 Kiel, Germany.

Most patients with pancreatic ductal adenocarcinoma (PDAC) undergoing curative resection relapse within months, often with liver metastases. The hepatic microenvironment determines induction and reversal of dormancy during metastasis. Both tumor growth and metastasis depend on the Tumor necrosis factor (TNF)-related apoptosis-inducing ligand-receptor 2 (TRAIL-R2). Read More

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IL-17 and TNF-α co-operation contributes to the proinflammatory response of hepatic stellate cells.

Clin Exp Immunol 2019 10 6;198(1):111-120. Epub 2019 Jun 6.

Immunogenomics and Inflammation research Unit EA 4130, University of Lyon, Lyon, France.

Hepatic stellate cells (HSCs) have a central role in liver inflammation and fibrosis by producing inflammatory and fibrotic mediators. Their activation is regulated through direct cell-cell interactions, but also through systemic and local effects of soluble factors such as cytokines. The effects of the proinflammatory cytokines interleukin (IL)-17 and tumor necrosis factor (TNF)-α and cell interactions with hepatocytes on HSC activation were assessed. Read More

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October 2019

Granulocyte-macrophage colony-stimulating factor inactivation in CAR T-cells prevents monocyte-dependent release of key cytokine release syndrome mediators.

J Biol Chem 2019 04 25;294(14):5430-5437. Epub 2019 Feb 25.

From Cellectis, Inc., 430 East 29th St., New York, New York 10016 and

Chimeric antigen receptor T-cell (CAR T-cell) therapy has been shown to be clinically effective for managing a variety of hematological cancers. However, CAR T-cell therapy is associated with multiple adverse effects, including neurotoxicity and cytokine release syndrome (CRS). CRS arises from massive cytokine secretion and can be life-threatening, but it is typically managed with an anti-IL-6Ra mAb or glucocorticoid administration. Read More

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Inhibition of inflammatory response in human keratinocytes by magnetic nanoparticles functionalized with PBP10 peptide derived from the PIP2-binding site of human plasma gelsolin.

J Nanobiotechnology 2019 Feb 2;17(1):22. Epub 2019 Feb 2.

Department of Microbiological and Nanobiomedical Engineering, Medical University of Bialystok, Mickiewicza 2c, 15-222, Bialystok, Poland.

Background: Human plasma gelsolin (pGSN) is a multifunctional actin-binding protein involved in a variety of biological processes, including neutralization of pro-inflammatory molecules such as lipopolysaccharide (LPS) and lipoteichoic acid (LTA) and modulation of host inflammatory response. It was found that PBP10, a synthetic rhodamine B-conjugated peptide, based on the phosphoinositide-binding site of pGSN, exerts bactericidal activity against Gram-positive and Gram-negative bacteria, interacts specifically with LPS and LTA, and limits microbial-induced inflammatory effects. The therapeutic efficiency of PBP10 when immobilized on the surface of iron oxide-based magnetic nanoparticles was not evaluated, to date. Read More

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February 2019

Chondroitin sulfate inhibits secretion of TNF and CXCL8 from human mast cells stimulated by IL-33.

Biofactors 2019 Jan 6;45(1):49-61. Epub 2018 Dec 6.

Laboratory of Molecular Immunopharmacology and Drug Discovery, Department of Immunology, Tufts University School of Medicine, Boston, MA, USA.

Glycosaminoglycans (GAGs) are linear, highly negatively charged carbohydrate chains present in connective tissues. Chondroitin sulfate (CS) and heparin (Hep) are also found in the numerous secretory granules of mast cells (MC), tissue immune cells involved in allergic and inflammatory reactions. CS and Hep may inhibit secretion of histamine from rat connective tissue MC, but their effect on human MC remains unknown. Read More

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January 2019

Design and development of pH-responsive polyurethane membranes for intravaginal release of nanomedicines.

Acta Biomater 2018 12 6;82:12-23. Epub 2018 Oct 6.

Biomedical Engineering, Faculty of Engineering, University of Manitoba, Winnipeg, Manitoba, Canada; Department of Biosystems Engineering, Faculty of Agricultural and Food Sciences, University of Manitoba, Winnipeg, Manitoba, Canada. Electronic address:

The objective of this study was to develop and characterize a novel intravaginal membrane platform for pH-triggered release of nanoparticles (NPs), which is essential for efficient intravaginal delivery of certain effective but acid-labile therapeutic agents for sexually transmitted infections, such as small interfering RNA (siRNA). A pH-responsive polyurethane (PU) was electrospun into a porous nanofibrous membrane. The diameters of the fibers, as well as the thickness and pore sizes of the membrane under dry and wet conditions (pH 4. Read More

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December 2018

TLR4 triggered complex inflammation in human pancreatic islets.

Biochim Biophys Acta Mol Basis Dis 2019 01 1;1865(1):86-97. Epub 2018 Oct 1.

University of Bremen, Center for Biomolecular Interactions Bremen, Germany. Electronic address:

Type 2 Diabetes (T2D) is strongly associated with obesity and inflammation. Toll-like receptor-4 (TLR-4) is the major pro-inflammatory pathway with its ligands and downstream products increased systemically in T2D and in at-risk individuals. Detailed mechanisms of the complex proinflammatory response in pancreatic islets remain unknown. Read More

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January 2019

Histone deacetylase 6 regulated expression of IL-8 is involved in the doxorubicin (Dox) resistance of osteosarcoma cells via modulating ABCB1 transcription.

Eur J Pharmacol 2018 Dec 28;840:1-8. Epub 2018 Sep 28.

Department of Musculoskeletal Surgery, Fudan University Shanghai Cancer Center, Shanghai 200032, China. Electronic address:

Emerging evidence shows that cytokines such as interleukins (ILs) are involved in the progression and chemoresistance of multiple tumors, including osteosarcoma (OS). Our present study established the doxorubicin (Dox) resistant human OS MG-63 and HOS cells and named them MG-63/Dox and HOS/Dox, respectively. The expression of IL-8, while not VEGFA, IL-32, or IL-34, was significantly increased in OS/Dox cells as compared with that in the parental cells. Read More

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December 2018

Proteasome inhibition induces IKK-dependent interleukin-8 expression in triple negative breast cancer cells: Opportunity for combination therapy.

PLoS One 2018 8;13(8):e0201858. Epub 2018 Aug 8.

Department of Biological Sciences, St. John's University, New York, New York, United States of America.

Triple negative breast cancer (TNBC) cells express increased levels of the pro-inflammatory and pro-angiogenic chemokine interleukin-8 (IL-8, CXCL8), which promotes their proliferation and migration. Because TNBC patients are unresponsive to current targeted therapies, new therapeutic strategies are urgently needed. While proteasome inhibition by bortezomib (BZ) or carfilzomib (CZ) has been effective in treating hematological malignancies, it has been less effective in solid tumors, including TNBC, but the mechanisms are incompletely understood. Read More

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January 2019

The Anti-Inflammatory Activities of Propionibacterium acnes CAMP Factor-Targeted Acne Vaccines.

J Invest Dermatol 2018 11 30;138(11):2355-2364. Epub 2018 Jun 30.

Department of Dermatology, School of Medicine, University of California, San Diego, California, USA; Department of Biomedical Sciences and Engineering, National Central University, Jhongli, Taiwan; Moores Cancer Center; University of California, San Diego, California, USA. Electronic address:

Inflammatory acne vulgaris afflicts hundreds of millions of people globally. Propionibacterium acnes, an opportunistic skin bacterium, has been linked to the pathogenesis of acne vulgaris. Our results show that a secretory Christie-Atkins-Munch-Petersen (CAMP) factor of P. Read More

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November 2018

Synergistic effect of interleukin-17 and tumour necrosis factor-α on inflammatory response in hepatocytes through interleukin-6-dependent and independent pathways.

Clin Exp Immunol 2018 08 31;193(2):221-233. Epub 2018 May 31.

Immunogenomics and Inflammation Research Unit EA 4130, University of Lyon.

The proinflammatory cytokines interleukin (IL)-17 and tumour necrosis factor (TNF)-α are targets for treatment in many chronic inflammatory diseases. Here, we examined their role in liver inflammatory response compared to that of IL-6. Human hepatoma cells (HepaRG, Huh7. Read More

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RIPK1 downregulation in keratinocyte enhances TRAIL signaling in psoriasis.

J Dermatol Sci 2018 Jul 10;91(1):79-86. Epub 2018 Apr 10.

Department of Dermatology, Asahikawa Medical University, Japan.

Background: Psoriasis, a common inflammatory skin disorder characterized by scaly erythema and plaques, is induced by dysregulation of dendritic cell- and T cell-mediated immune reaction. Receptor-interacting protein kinase 1 (RIPK1) regulates inflammatory signaling in response to stimuli such as TNF-α, TRAIL, and TLRs, resulting in apoptosis, necroptosis and NF-κB activation. However, the physiological relevance in human epidermis remains elusive. Read More

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Chlamydia trachomatis inhibits the production of pro-inflammatory cytokines in human PBMCs through induction of IL-10.

J Med Microbiol 2018 Feb;67(2):240-248

Department of clinical laboratory, The first clinical medical college of Yangtze university and the first people's hospital of Jingzhou, Jingzhou 434000, Hubei Province, PR China.

Purpose: Previous research demonstrated that IL-10 was up-regulated in Chlamydia trachomatis-infected cells and that exogenous IL-10 was able to inhibit the secretion of pro-inflammatory cytokines by infected cells. However, the mechanisms are not well understood. The aim of this study was to investigate the mechanisms for up-regulation of IL-10 and inhibition of pro-inflammatory cytokine secretion in C. Read More

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February 2018

Dual Blockade of Interleukin-1 and Interleukin-17A Reduces Murine Arthritis Pathogenesis but Also Leads to Spontaneous Skin Infections in Nonhuman Primates.

J Pharmacol Exp Ther 2018 03 8;364(3):474-484. Epub 2018 Jan 8.

AbbVie Bioresearch Center, Worcester, Massachusetts (M.C.R., L.H., T.T.Z., S.B., P.F.S., C.A.C., C.T.); and AbbVie Deutschland, Ludwigshafen, Germany (G.B.).

Despite the efficacy of biologics for treatment of rheumatoid arthritis (RA), many patients show inadequate responses and likely require neutralization of multiple mediators. Neutralization of both interleukin (IL)-1 and IL-17A with monoclonal antibodies showed greater efficacy than either agent alone in a mouse arthritis model with cooperative inhibition of key inflammatory factors, IL-6, granulocyte colony-stimulating factor (G-CSF), and CXC chemokine ligand (CXCL)1. Given the potential clinical benefit in RA, we generated a human dual variable domain antibody Ig, ABBV-615, capable of simultaneous binding and neutralization of IL-1 and IL-17A. Read More

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Neutralization of IL-8 decreases tumor PMN-MDSCs and reduces mesenchymalization of claudin-low triple-negative breast cancer.

JCI Insight 2017 11 2;2(21). Epub 2017 Nov 2.

The complex signaling networks of the tumor microenvironment that facilitate tumor growth and progression toward metastatic disease are becoming a focus of potential therapeutic options. The chemokine IL-8 is overexpressed in multiple cancer types, including triple-negative breast cancer (TNBC), where it promotes the acquisition of mesenchymal features, stemness, resistance to therapies, and the recruitment of immune-suppressive cells to the tumor site. The present study explores the utility of a clinical-stage monoclonal antibody that neutralizes IL-8 (HuMax-IL8) as a potential therapeutic option for TNBC. Read More

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November 2017