54 results match your criteria netrin-1 concentrations


Homogenous overexpression of the extracellular matrix protein Netrin-1 in a hollow fiber bioreactor.

Appl Microbiol Biotechnol 2021 Aug 3;105(14-15):6047-6057. Epub 2021 Aug 3.

Department of Chemistry, University of Manitoba, Winnipeg, Manitoba, Canada.

The production of recombinant proteins for functional and biophysical studies, especially in the field of structural determination, still represents a challenge as high quality and quantities are needed to adequately perform experiments. This is in part solved by optimizing protein constructs and expression conditions to maximize the yields in regular flask expression systems. Still, work flow and effort can be substantial with no guarantee to obtain improvements. Read More

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Increased serum concentration of netrin-1 after intravitreal bevacizumab injection: is it a compensatory mechanism to counteract drug side effects?

BMC Ophthalmol 2021 May 31;21(1):243. Epub 2021 May 31.

Recep Tayyip Erdoğan University, Zihni Derin Yerleşkesi - Fener Mahallesi, 53100, Merkez/Rize, Turkey.

Background: To evaluate alterations in the serum concentrations of vascular endothelial growth factor (VEGF) and netrin-1 after intravitreal bevacizumab (BCZ) injection for the treatment of diabetic macular edema (DME).

Methods: This prospective case-control study included a total of 50 participants assigned to one of three groups, including 10 individuals with DME and non-proliferative diabetic retinopathy (NPDR), 13 with DME, and proliferative diabetic retinopathy (PDR), and 27 healthy individuals as a control group. Serum VEGF and netrin-1 concentrations were measured by enzyme-linked immunosorbent assays (ELISAs) immediately before, as well as 1 week and 1 month after, intravitreal BCZ injection. Read More

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Serum netrin-1 as a potential biomarker for functional outcome of traumatic brain injury.

Clin Chim Acta 2021 Jul 16;518:22-27. Epub 2021 Mar 16.

Liangzhu Hospital of Yuhang District of Hangzhou City, 1657 Moganshan Road, 311113 Hangzhou, China.

Background: Decreased serum netrin-1 concentrations have been found after acute brain injury. We investigated the role of serum netrin in prognosis of traumatic brain injury (TBI).

Methods: In this prospective and observational study, enzyme-linked immunosorbent assay was used to detect serum netrin-1 concentrations in 50 mild TBI patients (Glasgow coma scale (GCS) score, 13-15), 83 moderate TBI patients (GCS score, 9-12), 69 severe TBI patients (GCS score, 3-8) and 50 healthy controls. Read More

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Netrin-1 and clusterin: Innovative potential diagnostic biomarkers for early renal damage in β-thalassemia major children.

IUBMB Life 2021 05 23;73(5):800-810. Epub 2021 Mar 23.

Medical Biochemistry & Molecular Biology Department, Faculty of Medicine, Tanta University, Tanta, Egypt.

Background: Children with β-thalassemia major (β-TM) suffer from tubular dysfunction even before the onset of any renal impairment symptoms and/or clinical signs. Therefore, identifying innovative biomarkers allowing early renal damage detection has focused attention.

Aim: This study aims to preliminary assess Netrin-1(NTN-1) and clusterin (CLU) in β-TM children and explore their possible roles as surrogate noninvasive biomarkers of renal tubular dysfunction. Read More

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Macrophage-derived netrin-1 contributes to endometriosis-associated pain.

Ann Transl Med 2021 Jan;9(1):29

Department of Gynecology, Women's Hospital, Zhejiang University School of Medicine, Hangzhou, China.

Background: Endometriosis-associated pain can be considered a type of neuropathic pain. Netrin-1 is an axon guidance cue that regulates axonal attraction or rejection in neural injury and regeneration. However, whether netrin-1 plays a role in endometriosis-associated pain remains unclear. Read More

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January 2021

Urinary netrin-1 concentration in healthy full-term newborns.

Arch Med Sci 2021 5;17(1):47-52. Epub 2021 Jan 5.

Department of Pediatrics and Nephrology, Medical University of Bialystok, Bialystok, Poland.

Introduction: Monitoring of renal function in acute kidney injury in the pediatric population is complicated by the lack of age-related reference values of new biomarkers. Urinary netrin-1 is a new marker to demonstrate early kidney damage. Netrin-1 has a molecular mass of 72 kDa. Read More

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January 2021

Decreased serum netrin-1 is associated with ischemic stroke: A case-control study.

Nutr Metab Cardiovasc Dis 2020 11 31;30(12):2328-2334. Epub 2020 Jul 31.

Department of Epidemiology, Tulane University School of Public Health and Tropical Medicine, New Orleans, LA, USA; Department of Medicine, Tulane University School of Medicine, New Orleans, LA, USA.

Background And Aims: Netrin-1 was a laminin-related protein involved in neurovascular protection, and we previously discovered that decreased serum netrin-1 was associated with poor prognosis of ischemic stroke. However, the relationship between serum netrin-1 level and the risk of ischemic stroke remains unclear. The aim of this study was to investigate the association between netrin-1 level and risk of ischemic stroke. Read More

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November 2020

Decreased Serum Netrin-1 as a Predictor for Post-Stroke Depression in Chinese Patients with Acute Ischemic Stroke.

J Stroke Cerebrovasc Dis 2020 Oct 28;29(10):105149. Epub 2020 Jul 28.

Department of Endocrinology, Mianzhu People's Hospital, Mianzhu 618200, Sichuan, China. Electronic address:

Background And Aims: Experimental studies demonstrated that netrin-1 (NT-1) is an important factor influencing the recovery after ischemic stroke. However, whether NT-1 levels were associated with the post-stroke depression (PSD) remains unclear. We aimed to evaluate the relationship between NT-1 levels and PSD at 3 months in patients with ischemic stroke. Read More

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October 2020

Serum netrin-1 concentrations are associated with clinical outcome in acute intracerebral hemorrhage.

Clin Chim Acta 2020 Sep 14;508:154-160. Epub 2020 May 14.

Department of Pain Medicine, Ruian People's Hospital, The Third Affiliated Hospital of Wenzhou Medical University, 108 Wansong Road, Ruian 325200, Zhejiang Province, China. Electronic address:

Background: Netrin-1 is an axon guidance protein, which can inhibit inflammatory reaction and stabilize the blood-brain barrier to protect against experimental brain injury. We evaluated the concentration of netrin-1 in acute intracerebral hemorrhage (ICH) patients and explored whether netrin-1 is a potential prognostic biomarker for ICH.

Methods: This study recruited a total of 126 ICH patients and 126 healthy controls. Read More

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September 2020

Molecular mechanism of nanoparticulate TiO induction of axonal development inhibition in rat primary cultured hippocampal neurons.

Environ Toxicol 2020 Aug 24;35(8):895-905. Epub 2020 Apr 24.

Department of Biochemistry and Molecular Biology, School of Basic Medical and Biological Sciences, Soochow University, Suzhou, China.

Numerous studies have demonstrated the in vitro and in vivo neurotoxicity of nanoparticulate titanium dioxide (nano-TiO ), a mass-produced material for a large number of commercial and industrial applications. The mechanism of nano-TiO -induced inhibition of axonal development, however, is still unclear. In our study, primary cultured hippocampal neurons of 24-hour-old fetal Sprague-Dawley rats were exposed to 5, 15, or 30 μg/mL nano-TiO for 6, 12, and 24 hours, and the toxic effects of nano-TiO exposure on the axons development were detected and its molecular mechanism investigated. Read More

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[Expression of Netrin-1 in Patients with Acute Lymphoblastic Leukemia and Its Clinical Significance].

Zhongguo Shi Yan Xue Ye Xue Za Zhi 2020 Apr;28(2):385-393

Department of Hematology & Oncology, Children's Hospital Affiliated to Chongqing Medical University,Key Laboratory of Child Development and Disorders of Ministry of Education,National Center for Clinical Medical Research on Child Health and Diseases (Chongqing),National International Scientific and Technological Cooperation Base for Major Diseases of Child Development, Chongqing Key Laboratory of Pediatrics,Chongqing 400014, China,E-mail:

Objective: To investigate the correlation of the Netrin-1 expression level with the clinical characteristics in children with acute lymphoblastic leukemia (ALL) and to explore its possible regulatory mechanism.

Methods: ELISA was used to detect the expression level of Netrin-1 in peripheral blood serum from 48 child ALL patients (newly diagnosed, recurrent), and its relevance with clinical indicators was statistically analyzed. The blood serum samples from 27 children with non malignant hematological diseases were choosen as controls. Read More

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Promotion of Neuronal Guidance Growth by Aminated Graphene Oxide via Netrin-1/Deleted in Colorectal Cancer Signaling.

ACS Chem Neurosci 2020 02 10;11(4):604-614. Epub 2020 Feb 10.

Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education, School of Biological Science and Medical Engineering , Beihang University , Beijing 100083 , China.

Promotion of neurite outgrowth and synapse formation is a key step for nervous tissue regeneration. It is important for finding a new biomaterial to guide neuron growth to target neurons. Aminated graphene oxide (NH-GO) displays electrical properties and dispersibility, which may change the surface charge of neurons and further activate neuronal excitement. Read More

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February 2020

Netrin-1 and the Grade of Atherosclerosis Are Inversely Correlated in Humans.

Arterioscler Thromb Vasc Biol 2020 02 5;40(2):462-472. Epub 2019 Dec 5.

Einthoven Laboratory for Vascular and Regenerative Medicine, Department of Internal Medicine, Leiden University Medical Center, the Netherlands (D.V., A.J.v.Z., J.M.v.G.).

Objective: Netrin-1 has been shown to play a role in the initiation of atherosclerosis in mice models. However, little is known about the role of Netrin-1 in humans. We set out to study whether Netrin-1 is associated with different stages of atherosclerosis. Read More

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February 2020

Netrin-1 and its receptor Unc5b as markers of periodontal disease.

J Periodontal Res 2020 Apr 25;55(2):267-276. Epub 2019 Nov 25.

Faculty of Dentistry, Research Center, Selcuk University, Konya, Turkey.

Objectives: The aim of this present study was to evaluate the levels of Netrin-1 and Unc5b in periodontal health and disease.

Background: Netrin-1, acting via its receptor UNC5b, regulates the inflammatory response and takes apart in bone destructive diseases.

Methods: Samples of gingival crevicular fluid (GCF), whole saliva, and serum were taken from systemically healthy, nonsmoking 20 periodontitis , 20 gingivitis patients, and 20 periodontally healthy subjects at baseline and 4 weeks after nonsurgical periodontal treatment (NSPT). Read More

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DCC-related developmental effects of abused- versus therapeutic-like amphetamine doses in adolescence.

Addict Biol 2020 07 13;25(4):e12791. Epub 2019 Jun 13.

Department of Psychiatry and Department of Neurology and Neurosurgery, McGill University, Douglas Mental Health University Institute, Montreal, Quebec, Canada.

The guidance cue receptor DCC controls mesocortical dopamine development in adolescence. Repeated exposure to an amphetamine regimen of 4 mg/kg during early adolescence induces, in male mice, downregulation of DCC expression in dopamine neurons by recruiting the Dcc microRNA repressor, microRNA-218 (miR-218). This adolescent amphetamine regimen also disrupts mesocortical dopamine connectivity and behavioral control in adulthood. Read More

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Decreased Netrin-1 and Correlated Th17/Tregs Balance Disorder in Aβ Induced Alzheimer's Disease Model Rats.

Front Aging Neurosci 2019 28;11:124. Epub 2019 May 28.

Department of Neurology, The First Affiliated Hospital of Harbin Medical University, Harbin, China.

There is increasing evidence indicating that inflammation represents a key pathological component of Alzheimer's disease (AD). A possible factor that may contribute to this process is netrin-1, a neuronal guidance molecule. This molecule has been shown to exert an unexpected immunomodulatory function. Read More

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Serum netrin-1 serves as a prognostic biomarker of aneurysmal subarachnoid hemorrhage.

Clin Chim Acta 2019 Aug 29;495:294-300. Epub 2019 Apr 29.

Department of Neurosurgery, The Zhuji People's Hospital of Zhejiang Province, 9 Jianmin Road, Zhuji 311800, China. Electronic address:

Background: Netrin-1 exhibits anti-inflammatory properties. Netrin-1 could alleviate brain injury of subarachnoid hemorrhage (SAH) rat. This study was designed to discern the utility of serum netrin-1 as a biomarker for assessing the severity and prognosis of patients with aneurysmal SAH. Read More

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Netrin‑1 interference potentiates epithelial‑to‑mesenchymal transition through the PI3K/AKT pathway under the hypoxic microenvironment conditions of non‑small cell lung cancer.

Int J Oncol 2019 Apr 15;54(4):1457-1465. Epub 2019 Feb 15.

Department of Respiratory Medicine, Τhe Second Hospital Affiliated to Dalian Medical University, Dalian, Liaoning 116027, P.R. China.

Netrin‑1 is overexpressed in several types of cancer. However, whether netrin‑1 can potentiate hypoxia‑induced tumor progression in lung cancer has not been reported to date. Thus, the objective of the present study was to investigate whether netrin‑1 regulates cancer cell migration and invasion under hypoxic conditions in lung cancer and explore the underlying mechanism. Read More

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Neuroprotection Exerted by Netrin-1 and Kinesin Motor KIF1A in Secondary Brain Injury following Experimental Intracerebral Hemorrhage in Rats.

Front Cell Neurosci 2017 11;11:432. Epub 2018 Jan 11.

Department of Neurosurgery and Brain and Nerve Research Laboratory, The First Affiliated Hospital of Soochow University, Suzhou, China.

Binding of extracellular netrin-1 to its receptors, deleted in colorectal cancer (DCC) and uncoordinated gene 5H2 (UNC5H2), inhibits apoptosis mediated by these receptors. A neuron-specific kinesin motor protein, KIF1A, has been shown to participate in netrin-1 secretion. This study aimed to identify the roles of netrin-1 and KIF1A in secondary brain injury after intracerebral hemorrhage (ICH) and the potential mechanisms. Read More

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January 2018

Semaphorin-3A and Netrin-1 predict the development of kidney injury in children with congenital hydronephrosis.

Scand J Clin Lab Invest 2018 Feb - Apr;78(1-2):55-61. Epub 2017 Dec 3.

e Nephrology Department , The First Affiliated Hospital of Zhengzhou University , Zhengzhou , PR China.

Congenital obstructive nephropathy is amongst the main causes of chronic renal failure in children. Early diagnosis and initiation of the treatment will delay progressive renal tubular atrophy and interstitial fibrosis with the loss of nephrons. The aim of this study was to evaluate whether urinary (u) semaphorin-3A (SEMA-3A) and Netrin-1 may be potential biomarkers in children with congenital hydronephrosis due to ureteropelvic junction obstruction (UPJO). Read More

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September 2018

Activation of Inflammatory and Pro-Thrombotic Pathways in Acute Stress Cardiomyopathy.

Front Cardiovasc Med 2017 3;4:49. Epub 2017 Aug 3.

Department of Medicine, University of Massachusetts Medical School, Worcester, MA, United States.

Stress cardiomyopathy (SCM) is a unique cardiac disorder that more often occurs in women. SCM presents in a similar fashion as acute myocardial infarction (AMI), with chest pain, ECG changes, and congestive heart failure. The primary distinguishing feature is the absence of thrombotic coronary occlusion in SCM. Read More

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RBP4/Lp-PLA2/Netrin-1 signaling regulation of cognitive dysfunction in diabetic nephropathy complicated with silent cerebral infarction.

Exp Clin Endocrinol Diabetes 2017 Sep 13;125(8):547-553. Epub 2017 Jul 13.

Departments of Pathophysiology, Basic Medical College, Chongqing Medical University, Chongqing, China.

To investigate the RBP4/Lp-PLA/Netrin-1 signal regulation of cognitive function impairment in diabetic nephropathy patients with silent cerebral infarction (SCI). One-hundred patients newly diagnosed with diabetic nephropathy patients were included. The patients were divided into SCI group and NSCI group according to the radiological data. Read More

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September 2017

Netrin-1 and multiple sclerosis: a new biomarker for neuroinflammation?

Eur J Neurol 2017 09 5;24(9):1108-1115. Epub 2017 Jul 5.

Instituto de Biología y Genética Molecular, CSIC - Universidad de Valladolid, Valladolid, Spain.

Background And Purpose: Netrin-1, an axon guidance protein, reduces serum levels of pro-inflammatory mediators and stabilizes the blood-brain barrier limiting the entrance of immune cells into the central nervous system. The aim was to investigate its presence in the experimental autoimmune encephalomyelitis (EAE) model and in multiple sclerosis (MS) patients with and without clinical activity.

Methods: Netrin-1 levels were evaluated in EAE mouse tissues. Read More

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September 2017

Influence of Netrin-1 on reinnervation of laryngeal muscles following recurrent laryngeal nerve injury.

Neurosci Lett 2017 Jul 17;653:244-249. Epub 2017 May 17.

Department of Otolaryngology - Head and Neck Surgery, Columbia University Medical Center, New York, NY, USA. Electronic address:

Following recurrent laryngeal nerve (RLN) injury, recovery results in poor functional restitution of the paralyzed vocal fold. Netrin-1 has been found to be upregulated in the rat posterior cricoarytenoid muscle (PCA) during nerve regeneration. We evaluated the effect of ectopic Netrin-1 in the PCA during RLN reinnervation. Read More

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Large-scale microfluidic gradient arrays reveal axon guidance behaviors in hippocampal neurons.

Microsyst Nanoeng 2017 8;3:17003. Epub 2017 May 8.

Department of Bioengineering, University of Washington, 3720 15th Ave NE, Foege N423-A, Seattle, WA 98195, USA.

High-throughput quantitative approaches to study axon growth behaviors have remained a challenge. We have developed a 1024-chamber microfluidic gradient generator array that enables large-scale investigations of axon guidance and growth dynamics from individual primary mammalian neurons, which are exposed to gradients of diffusible molecules. Our microfluidic method (a) generates statistically rich data sets, (b) produces a stable, reproducible gradient with negligible shear stresses on the culture surface, (c) is amenable to the long-term culture of primary neurons without any unconventional protocol, and (d) eliminates the confounding influence of cell-secreted factors. Read More

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Netrin-1 acts as a non-canonical angiogenic factor produced by human Wharton's jelly mesenchymal stem cells (WJ-MSC).

Stem Cell Res Ther 2017 02 28;8(1):43. Epub 2017 Feb 28.

Laboratory of Stem Cells and Developmental Biology, Faculty of Sciences, University of Chile, Santiago de Chile, Chile.

Background: Angiogenesis, the process in which new blood vessels are formed from preexisting ones, is highly dependent on the presence of classical angiogenic factors. Recent evidence suggests that axonal guidance proteins and their receptors can also act as angiogenic regulators. Netrin, a family of laminin-like proteins, specifically Netrin-1 and 4, act via DCC/Neogenin-1 and UNC5 class of receptors to promote or inhibit angiogenesis, depending on the physiological context. Read More

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February 2017

The Netrin-4/ Neogenin-1 axis promotes neuroblastoma cell survival and migration.

Oncotarget 2017 Feb;8(6):9767-9782

Laboratory of Stem Cells and Developmental Biology, Faculty of Sciences, Universidad de Chile, Santiago, Chile.

Neogenin-1 (NEO1) is a transmembrane receptor involved in axonal guidance, angiogenesis, neuronal cell migration and cell death, during both embryonic development and adult homeostasis. It has been described as a dependence receptor, because it promotes cell death in the absence of its ligands (Netrin and Repulsive Guidance Molecule (RGM) families) and cell survival when they are present. Although NEO1 and its ligands are involved in tumor progression, their precise role in tumor cell survival and migration remain unclear. Read More

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February 2017

Potential effect of mechano growth factor E-domain peptide on axonal guidance growth in primary cultured cortical neurons of rats.

J Tissue Eng Regen Med 2018 01 8;12(1):70-79. Epub 2017 Jun 8.

Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education, School of Biological Science and Medical Engineering, Beihang University, Beijing, 100191, China.

Establishing appropriate synaptic connections and plasticity is a critical need in neuronal regeneration and development. Mechano growth factor (MGF) and its C-terminal E-domain peptide with 24 amino acids, MGF-Ct24E, are potential neuroprotective agents. Our preliminary study indicates that Netrin-1 can guide axonal growth and its expression is sensitive to MGF, but how MGF regulates the expression of Netrin-1 and its receptor DCC is still unclear. Read More

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January 2018

The value of plasma netrin-1 in non-small cell lung cancer patients as diagnostic and prognostic biomarker.

Tumour Biol 2016 Sep 11;37(9):11903-11907. Epub 2016 Apr 11.

Department of Medical Oncology, School of Medicine, Bezmialem University, Istanbul, Turkey.

Netrin-1 is found to be elevated and purposive as a diagnostic biomarker in many human cancers. We evaluated serum netrin-1 concentrations in patients with advanced non-small cell lung cancer compared with those in a healthy group. Thirty patients with advanced non-small cell lung cancer and 30 healthy people were included in the study. Read More

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September 2016

Dual actions of Netrin-1 on islet insulin secretion and immune modulation.

Clin Sci (Lond) 2016 11 12;130(21):1901-11. Epub 2016 Aug 12.

Tianjin Key Laboratory of Biomaterial Research, Institute of Biomedical Engineering, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300192, China

Netrin-1 is typically known as a neural guidance cue, which has been implicated in pancreas development. Since regenerative, angiogenic and anti-inflammatory properties of Netrin-1 have been reported in multiple tissues, we have investigated the potential role of Netrin-1 in the endocrine islet and its implication in mice with high-fat diet (HFD)/streptozotocin (STZ)-induced diabetes. Effects of exogenous Netrin-1 on β-cell [Ca(2+)]i, cyclic AMP (cAMP) and insulin production were assessed in vitro The long-term impact of Netrin-1 treatment was then evaluated in HFD/STZ-induced diabetic mice by subcutaneous implantation of osmotic minipumps which release Netrin-1 in a sustained manner for 4 weeks. Read More

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November 2016