78 results match your criteria nestin mbp

Developmental neurotoxicity of antimony (Sb) in the early life stages of zebrafish.

Ecotoxicol Environ Saf 2021 May 8;218:112308. Epub 2021 May 8.

Key Laboratory for Biorheological Science and Technology of Ministry of Education, State and Local Joint Engineering Laboratory for Vascular Implants, Bioengineering College of Chongqing University, Chongqing 400030, China. Electronic address:

Accumulating studies have revealed the toxicity of antimony (Sb) to soil-dwelling and aquatic organisms at the individual level. However, little is known about the neurotoxic effects of antimony and its underlying mechanisms. To assess this issue, we investigated the neurotoxicity of antimony (0, 200, 400, 600 and 800 mg/L) in zebrafish embryos. Read More

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Microvesicles from Schwann-Like Cells as a New Biomaterial Promote Axonal Growth.

J Biomed Nanotechnol 2021 Feb;17(2):291-302

Jiangsu Key Laboratory of Medical Science and Laboratory Medicine, School of Medicine, Jiangsu University, Zhenjiang 212013, Jiangsu, China.

Schwann cells promote axonal regeneration following peripheral nerve injury. However, in terms of clinical treatment, the therapeutic effects of Schwann cells are limited by their source. The transmission of microvesicles from neuroglia cells to axons is a novel communication mechanism in axon regeneration. Read More

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February 2021

Generation of High-Yield, Functional Oligodendrocytes from a c- Immortalized Neural Cell Line, Endowed with Staminal Properties.

Int J Mol Sci 2021 Jan 23;22(3). Epub 2021 Jan 23.

Department of Environmental, Biological and Pharmaceutical Sciences and Technologies, University of Campania "Luigi Vanvitelli", 81100 Caserta, Italy.

Neural stem cells represent a powerful tool to study molecules involved in pathophysiology of Nervous System and to discover new drugs. Although they can be cultured and expanded in vitro as a primary culture, their use is hampered by their heterogeneity and by the cost and time needed for their preparation. Here we report that mes-c-myc A1 cells (A1), a neural cell line, is endowed with staminal properties. Read More

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January 2021

Comparison of effects of high- and low-frequency electromagnetic fields on proliferation and differentiation of neural stem cells.

Neurosci Lett 2021 01 29;741:135463. Epub 2020 Oct 29.

Department of Rehabilitation Medicine, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangdong Provincial Institute of Geriatrics, Guangzhou, China. Electronic address:

To compare the effects of high- (HF-EMF) and low-frequency electromagnetic fields (LF-EMF) on the proliferation and differentiation of neural stem cells (NSCs). NSCs were obtained from SD rat hippocampus and cultured in suspension and adherent differentiation media. NSCs were exposed to LF-EMF (5 m T, 50 Hz, 30 min daily), HF-EMF (maximum magnetic induction 2. Read More

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January 2021

Induced neural stem cells-derived spinal cord progenitor cells as stable stage for rapid and efficient generation of human oligodendrocytes and motor neurons.

Connect Tissue Res 2021 Mar 7;62(2):206-214. Epub 2020 May 7.

Department of Neurosurgery, First Clinical Medical College of Shanxi Medical University , Taiyuan, Shanxi Province, China.

Previous studies have shown that oligodendrocytes and motor neurons have the same progenitors in the ventral spinal cord called spinal cord progenitor cells marked by oligodendrocyte lineage transcription factor 2 (Olig2). However, it is difficult to identify the spinal cord progenitor cell in vitro as they are present transiently and further transform into other neuronal (interneuron) and glial (oligodendrocyte) lineages during development. In the present study, we try to generated Olig2 spinal cord progenitor cells from human induced neural stem cells (iNSCs) and identify those spinal cord progenitor cells in vitro Human peripheral blood mononuclear cells (PBMCs) were converted into induced neural stem cells (iNSCs), after they were identified by immunostaining using neural stem cell markers such as Nestin, Sox1, Sox2, iNSCs were transformed into Olig2 spinal cord progenitor cells in 3 weeks by using small molecules. Read More

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Effects of copper-impregnated collagen implants on local pro- and anti-inflammatory and regenerative tissue reactions following implantation in rats.

J Biomed Mater Res A 2020 04 27;108(4):871-881. Epub 2019 Dec 27.

Department of Surgery, University Medical Center Greifswald, Greifswald, Germany.

Combining collagen, an established regenerative biomaterial, and copper (Cu) with its known antibacterial and angiogenic effects could improve wound healing. However, Cu is also cytotoxic. Thus, this study aimed at examining the tissue reactions after simultaneous intramuscular implantation of collagen discs either without Cu (controls) or impregnated in 2, 20, or 200 mmol/L Cu acetate in 24 rats. Read More

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Isolation and Characterization of Neural Stem Cells from the Rat Inferior Colliculus.

Stem Cells Int 2019 29;2019:5831240. Epub 2019 Oct 29.

Department of Oto-Rhino-Laryngology, Plastic, Aesthetic and Reconstructive Head and Neck Surgery, Comprehensive Hearing Center, Universitätsklinikum Würzburg, Germany.

The inferior colliculus (IC) is a nucleus of the auditory pathway and its fourth relay station. It integrates afferent information from the superior olivary complex and the cochlear nucleus. To date, no causal therapeutic options are known for damaged neuronal structures in this area. Read More

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October 2019

Human gingival derived neuronal cells in the optimized caffeic acid hydrogel for hemitransection spinal cord injury model.

J Cell Biochem 2020 03 23;121(3):2077-2088. Epub 2019 Oct 23.

Department of Periodontology and Implantology, Faculty of Dental Sciences and Centre for Regenerative Medicine and Stem Cell Research, Sri Ramachandra Medical College and Research Institute, Chennai, India.

Spinal cord injury induces scar formation causes axonal damage that leads to the degeneration of axonal function. Still, there is no robust conceptual design to regenerate the damaged axon after spinal injury. Therefore, the present study demonstrates that human gingival derived neuronal stem cells (GNSCs) transplants in the injectable caffeic acid bioconjugated hydrogel (CBGH) helps to bridge the cavity and promote the engraftment and repopulation of transplants in the injured spinal tissue. Read More

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Bisphenol A triggers axonal injury and myelin degeneration with concomitant neurobehavioral toxicity in C57BL/6J male mice.

Toxicology 2019 12 28;428:152299. Epub 2019 Sep 28.

Molecular Toxicology Laboratory, Department of Medical Elementology and Toxicology, Jamia Hamdard (Hamdard University), New Delhi 110062, India. Electronic address:

Bisphenol A (BPA) is a ubiquitously distributed endocrine disrupting chemical (EDC). BPA exposure in humans has been a matter of concern due to its increased application in the products of day to day use. BPA has been reported to cause toxicity in almost all the vital organ systems even at a very low dose levels. Read More

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December 2019

Characterization of spontaneous spheroids from oral mucosa-derived cells and their direct comparison with spheroids from skin-derived cells.

Stem Cell Res Ther 2019 06 24;10(1):184. Epub 2019 Jun 24.

Department of Hard Tissue Research, Graduate School of Oral Medicine, Matsumoto Dental University, Shiojiri, Japan.

Background: Our group has developed a novel method for spontaneous spheroid formation using a specific low-adherence culture plate with around 90° water contact angle. In this study, this method was applied for oral mucosa-derived cells. First, the feasibility of spontaneous spheroid formation was tested. Read More

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Selective Neural Deletion of the Gene Reduces Irradiation-Induced Cerebellar White Matter Injury in the Juvenile Mouse Brain by Ameliorating Oligodendrocyte Progenitor Cell Loss.

Front Cell Neurosci 2019 31;13:241. Epub 2019 May 31.

Henan Key Laboratory of Child Brain Injury, Third Affiliated Hospital and Institute of Neuroscience, Zhengzhou University, Zhengzhou, China.

Radiotherapy is an effective tool for treating brain tumors, but irradiation-induced toxicity to the normal brain tissue remains a major problem. Here, we investigated if selective neural () deletion has a persistent effect on irradiation-induced juvenile mouse brain injury. Ten-day-old knockout under a nestin promoter (KO) mice and wild-type (WT) littermates were subjected to a single dose of 6 Gy whole-brain irradiation. Read More

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The development of an in vitro cerebral organoid model for investigating the pathomolecular mechanisms associated with the central nervous system involvement in Mitochondrial Neurogastrointestinal Encephalomyopathy (MNGIE).

Nucleosides Nucleotides Nucleic Acids 2018 27;37(11):603-617. Epub 2018 Dec 27.

a Molecular & Clinical Sciences Research Institute, St. George's, University of London, United Kingdom.

Mitochondrial neurogastrointestinal encephalomyopathy (MNGIE) is a rare disorder caused by mutations in the thymidine phosphorylase gene (TYMP), leading to secondary aberrations to the mitochondrial genome. The disease is characterised by gastrointestinal dysmotility, sensorimotor peripheral neuropathy and leukoencephalopathy. The understanding of the molecular mechanisms that underlie the central nervous system (CNS) is hindered by the lack of a representative disease model; to address this we have developed an in vitro 3-D cerebral organoid of MNGIE. Read More

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November 2019

Derivation of preoligodendrocytes from human-induced pluripotent stem cells through overexpression of microRNA 338.

J Cell Biochem 2019 06 23;120(6):9700-9708. Epub 2018 Dec 23.

Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.

MicroRNAs (miRNAs) control gene expression at the posttranscriptional level and have a critical role in many biological processes such as oligodendrocyte differentiation. Recent studies have shown that microRNA 338 (miR-338) is overexpressed during the oligodendrocyte development process in the central nervous system; this finding indicates a potentially important role for miR-338 in oligodendrocyte development. To evaluate this assumption, we studied the effect of miR-338 overexpression on promoting the differentiation of oligodendrocyte progenitor cells (OPCs), derived from human-induced pluripotent stem cells (hiPSC), into preoligodendrocyte. Read More

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Inhibition of Notch1 Signaling at the Subacute Stage of Stroke Promotes Endogenous Neurogenesis and Motor Recovery After Stroke.

Front Cell Neurosci 2018 7;12:245. Epub 2018 Aug 7.

Department of Radiology, Huashan Hospital, Fudan University, Shanghai, China.

It is still not clear whether Notch1 signaling inhibition can promote functional outcomes after stroke, given that it plays time-dependent roles in the sequential process of endogenous neurogenesis. The purpose of this study was to identify the appropriate time frame for Notch1 signaling inhibition according to the temporal evolution of Notch1 signaling activation and the responses of neural stem cells (NSCs), in order to target it for therapeutic intervention and stimulate neurorestorative strategies after stroke. Sprague-Dawley (SD) rats were subjected to 90-min of middle cerebral artery occlusion (MCAO). Read More

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Overexpression of miR-219 promotes differentiation of human induced pluripotent stem cells into pre-oligodendrocyte.

J Chem Neuroanat 2018 09 9;91:8-16. Epub 2018 Mar 9.

Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran. Electronic address:

Oligodendrocytes play critical roles in the central nervous system (CNS) thorough producing myelin sheaths around axons. There are a variety of approaches to produce oligodendrocytes in vitro and in vivo which are a subject of interest in many studies. A new approach to induce this differentiation is using microRNA 219 (miR-219). Read More

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September 2018

Developmental neurotoxicity of Microcystis aeruginosa in the early life stages of zebrafish.

Ecotoxicol Environ Saf 2018 Apr 2;151:35-41. Epub 2018 Jan 2.

College of Environment, Zhejiang University of Technology, Hangzhou 310032, PR China; Collaborative Innovation Center of Yangtze River Delta Region Green Pharmaceuticals, Zhejiang University of Technology, Hangzhou 310032, PR China. Electronic address:

Accumulating evidence suggests that cyanotoxins can exert neurotoxic effects on exposed aquatic organisms but most studies have focused on purified toxins rather than on the more complex effects of cyanobacterial blooms. To evaluate this issue in an environmentally relevant model, we assessed the developmental neurotoxicity induced by Microcystis aeruginosa on newly hatched zebrafish. After four days of exposure, the locomotor activity of zebrafish larvae was significantly decreased with increasing algae concentration. Read More

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Physical Exercise Promotes Novel Object Recognition Memory in Spontaneously Hypertensive Rats after Ischemic Stroke by Promoting Neural Plasticity in the Entorhinal Cortex.

Front Behav Neurosci 2017 8;11:185. Epub 2017 Nov 8.

Department of Rehabilitation Medicine, Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, China.

Cerebral ischemia leads to memory impairment, and several studies have indicated that physical exercise (PE) has memory-improving effects after ischemia. This study was designed to further explore the specific role of PE in novel object recognition (NOR) memory after stroke and the exact cortical regions in which memory is restored by PE. Spontaneously hypertensive rats (SHR) were subjected to transient middle cerebral artery occlusion (tMCAO) or sham surgery, followed by 26 days of PE starting on day 3 post-tMCAO. Read More

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November 2017

NOTCH Signaling Is Essential for Maturation, Self-Renewal, and Tri-Differentiation of In Vitro Derived Human Neural Stem Cells.

Cell Reprogram 2017 12 16;19(6):372-383. Epub 2017 Oct 16.

1 Cellular and Molecular Therapeutics Laboratory, Centre for Biomaterials, Cellular and Molecular Theranostics, Vellore Institute of Technology (VIT) University , Vellore, India .

Although neural stem cells (NSCs) have potential applications in treating neurological disorders, much still needs to be understood about the differentiation biology for their successful clinical translation. In this study, we aimed at deriving NSCs from human umbilical cord blood-derived mesenchymal stem cells (hUCB-MSCs) and explored the role of Notch signaling in the differentiation process. The hUCB-MSCs were characterized as per guidelines of the International Society of Cellular Therapy. Read More

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December 2017

Differentiation potential of neural stem cells derived from fetal sheep.

Anim Cells Syst (Seoul) 2017 3;21(4):233-240. Epub 2017 Aug 3.

Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, PR China.

Neural stem cells (NSCs) are multipotent stem cells that can differentiate into many cell types in vitro. In this study, we isolated and established an NSC line from fetal . Based on the results of immunofluorescence staining, NSCs expressed Nestin, Pax6 and MAP2. Read More

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Acute oligodendrocyte loss with persistent white matter injury in a third trimester equivalent mouse model of fetal alcohol spectrum disorder.

Glia 2017 08 18;65(8):1317-1332. Epub 2017 May 18.

Department of Neurosciences, University of New Mexico Health Sciences Center, Albuquerque, New Mexico.

Alcohol exposure during central nervous system (CNS) development can lead to fetal alcohol spectrum disorder (FASD). Human imaging studies have revealed significant white matter (WM) abnormalities linked to cognitive impairment in children with FASD; however, the underlying mechanisms remain unknown. Here, we evaluated both the acute and long-term impacts of alcohol exposure on oligodendrocyte number and WM integrity in a third trimester-equivalent mouse model of FASD, in which mouse pups were exposed to alcohol during the first 2 weeks of postnatal development. Read More

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Differentiation of human olfactory bulb-derived neural stem cells toward oligodendrocyte.

J Cell Physiol 2018 Feb 22;233(2):1321-1329. Epub 2017 Jun 22.

Institute of Cell Biology and Neurobiology, National Research Council of Italy, Rome, Italy.

In the central nervous system (CNS), oligodendrocytes are the glial element in charge of myelin formation. Obtaining an overall presence of oligodendrocyte precursor cells/oligodendrocytes (OPCs/OLs) in culture from different sources of NSCs is an important research area, because OPCs/OLs may provide a promising therapeutic strategy for diseases affecting myelination of axons. The present study was designed to differentiate human olfactory bulb NSCs (OBNSCs) into OPCs/OLs and using expression profiling (RT-qPCR) gene, immunocytochemistry, and specific protein expression to highlight molecular mechanism(s) underlying differentiation of human OBNSCs into OPCs/OLs. Read More

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February 2018

Capacity of Human Dental Follicle Cells to Differentiate into Neural Cells .

Stem Cells Int 2017 5;2017:8371326. Epub 2017 Feb 5.

Department of Maxillofacial Surgery, Nihon University School of Dentistry at Matsudo, Matsudo, Japan; Research Institute of Oral Science, Nihon University School of Dentistry at Matsudo, Matsudo, Japan.

The dental follicle is an ectomesenchymal tissue surrounding the developing tooth germ. Human dental follicle cells (hDFCs) have the capacity to commit to differentiation into multiple cell types. Here we investigated the capacity of hDFCs to differentiate into neural cells and the efficiency of a two-step strategy involving floating neurosphere-like bodies for neural differentiation. Read More

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February 2017

Tumour-associated glial host cells display a stem-like phenotype with a distinct gene expression profile and promote growth of GBM xenografts.

BMC Cancer 2017 Feb 7;17(1):108. Epub 2017 Feb 7.

Oncomatrix Research Lab, Department of Biomedicine, University of Bergen, Bergen, Norway.

Background: Little is known about the role of glial host cells in brain tumours. However, supporting stromal cells have been shown to foster tumour growth in other cancers.

Methods: We isolated stromal cells from patient-derived glioblastoma (GBM) xenografts established in GFP-NOD/scid mice. Read More

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February 2017

Transplanted miR-219-overexpressing oligodendrocyte precursor cells promoted remyelination and improved functional recovery in a chronic demyelinated model.

Sci Rep 2017 02 1;7:41407. Epub 2017 Feb 1.

Department of Cell Biology and Neurobiology, Xuzhou Key Laboratory of Neurobiology, Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Xuzhou Medical University, Xuzhou, 221009, China.

Oligodendrocyte precursor cells (OPCs) have the ability to repair demyelinated lesions by maturing into myelin-producing oligodendrocytes. Recent evidence suggests that miR-219 helps regulate the differentiation of OPCs into oligodendrocytes. We performed oligodendrocyte differentiation studies using miR-219-overexpressing mouse embryonic stem cells (miR219-mESCs). Read More

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February 2017

Angiogenic microspheres promote neural regeneration and motor function recovery after spinal cord injury in rats.

Sci Rep 2016 09 19;6:33428. Epub 2016 Sep 19.

Department of Neurobiology, Beijing Institute for Brain Disorders, Beijing Center of Neural Regeneration and Repair, Beijing Key Laboratory of Major Brain Disorders, Capital Medical University, 10 Xitoutiao, You An Men, Beijing 100069, China.

This study examined sustained co-delivery of vascular endothelial growth factor (VEGF), angiopoietin-1 and basic fibroblast growth factor (bFGF) encapsulated in angiogenic microspheres. These spheres were delivered to sites of spinal cord contusion injury in rats, and their ability to induce vessel formation, neural regeneration and improve hindlimb motor function was assessed. At 2-8 weeks after spinal cord injury, ELISA-determined levels of VEGF, angiopoietin-1, and bFGF were significantly higher in spinal cord tissues in rats that received angiogenic microspheres than in those that received empty microspheres. Read More

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September 2016

Myelin Basic Protein Regulates Primitive and Definitive Neural Stem Cell Proliferation from the Adult Spinal Cord.

Stem Cells 2017 02 23;35(2):485-496. Epub 2016 Sep 23.

Department of Surgery, University of Toronto, Toronto, Canada.

The adult mammalian forebrain comprises two distinct populations of neural stem cells (NSCs): rare, Oct4 positive, primitive NSCs (pNSCs) and definitive NSC (dNSC) which are more abundant and express GFAP. The pNSCs are upstream of the dNSCs in the neural stem cell lineage. Herein we show that pNSC and dNSC populations can also be isolated from the developing and adult spinal cord. Read More

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February 2017

Clonal Heterogeneity in the Neuronal and Glial Differentiation of Dental Pulp Stem/Progenitor Cells.

Stem Cells Int 2016 26;2016:1290561. Epub 2016 May 26.

Oral and Biomedical Sciences, School of Dentistry, Cardiff University, Heath Park, Cardiff CF14 4XY, UK.

Cellular heterogeneity presents an important challenge to the development of cell-based therapies where there is a fundamental requirement for predictable and reproducible outcomes. Transplanted Dental Pulp Stem/Progenitor Cells (DPSCs) have demonstrated early promise in experimental models of spinal cord injury and stroke, despite limited evidence of neuronal and glial-like differentiation after transplantation. Here, we report, for the first time, on the ability of single cell-derived clonal cultures of murine DPSCs to differentiate in vitro into immature neuronal-like and oligodendrocyte-like cells. Read More

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Isolation and characterization of tumorspheres from a recurrent pineoblastoma patient: Feasibility of a patient-derived xenograft.

Int J Oncol 2016 Aug 1;49(2):569-78. Epub 2016 Jun 1.

Department of Neurosurgery, Brain Tumor Center, Severance Hospital, Yonsei University College of Medicine, Seoul, Republic of Korea.

The existence of tumorspheres (TSs) might confer treatment resistance to pineoblastoma (PB). The existence of PB TSs with cellular immortalization potential has not yet been reported. We developed a procedure for isolating TSs from recurrent PB (rPB) and tested whether their properties made them suitable for use as a patient-derived xenograft (PDX). Read More

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Atrazine and its main metabolites alter the locomotor activity of larval zebrafish (Danio rerio).

Chemosphere 2016 Apr 21;148:163-70. Epub 2016 Jan 21.

College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou 310032, China. Electronic address:

Atrazine (ATZ) and its main chlorometabolites, i.e., diaminochlorotriazine (DACT), deisopropylatrazine (DIP), and deethylatrazine (DE), have been widely detected in aquatic systems near agricultural fields. Read More

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Scutellarin Alleviates Behavioral Deficits in a Mouse Model of Multiple Sclerosis, Possibly Through Protecting Neural Stem Cells.

J Mol Neurosci 2016 Feb 29;58(2):210-20. Epub 2015 Oct 29.

Key Laboratory of Stem Cells and Regenerative Medicine, Institute of Molecular and Clinical Medicine, Kunming Medical University, Kunming, Yunnan, People's Republic of China.

Scutellarin, a flavonoid extracted from an herbal medication (Erigeron breviscapus Hand-Mazz), has been shown to protect neurons against damage and to promote neurogenesis, and thus has therapeutic potential in the treatment of a variety of neurodegenerative diseases. Since neural stem cells (NSCs) could differentiate into myelin-producing oligodendrocytes, we speculate that scutellarin could also be used to treat multiple sclerosis (MS). In the current study, we examined potential effects of scutellarin using a mouse model of MS. Read More

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February 2016