285,752 results match your criteria nested-pcr method


MiR-128-3p suppresses tumor proliferation and metastasis via targeting CDC6 in hepatocellular carcinoma cells.

Tissue Cell 2021 Mar 23;72:101534. Epub 2021 Mar 23.

School of Medicine, Shaoxing University, China; Department of General Surgery, Xinchang People's Hospital, China. Electronic address:

Background: MicroRNAs (miRNAs) are known to be involved in the pathogenesis of various cancers. The present study devotes efforts to discover the role of miR-128-3p in hepatocellular carcinoma (HCC).

Methods: MiR-128-3p and cell division cycle 6 (CDC6) expressions in HCC tissue (n = 50) and adjacent normal tissue (n = 50) were detected by quantitative real-time polymerase chain reaction (qRT-PCR). Read More

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Clinical evaluation of a developed paper-based Dengue NS1 rapid diagnostic test in febrile illness patients.

Int J Infect Dis 2021 May 12. Epub 2021 May 12.

School of Bioresources and Technology, King Mongkut's University of Technology Thonburi, Bang Khun Thian, Bangkok 10150, Thailand. Electronic address:

Objectives: The aim of this study was to evaluate, a developed microfluidic paper based analytical device (DEN-NS1-PAD) based on rapid NS1 antigen test for diagnosing dengue at the point of care.

Methods: 219 serum samples from suspected dengue cases were tested with the developed DEN-NS1-PAD and commercial RDT by SD BIOLINE. The results were compared with the nested-PCR results. Read More

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CTRP3 promotes TNF-α-induced apoptosis and barrier dysfunction in salivary epithelial cells.

Cell Signal 2021 May 12:110042. Epub 2021 May 12.

Department of Physiology and Pathophysiology, Peking University School of Basic Medical Sciences, Key Laboratory of Molecular Cardiovascular Sciences, Ministry of Education, and Beijing Key Laboratory of Cardiovascular Receptors Research, Beijing, China. Electronic address:

Background: C1q/tumour necrosis factor-related protein 3 (CTRP3) plays important roles in metabolism and inflammatory responses in various cells and tissues. However, the expression and function of CTRP3 in salivary glands have not been explored.

Methods: The expression and distribution of CTRP3 were detected by western blot, polymerase chain reaction, immunohistochemical and immunofluorescence staining. Read More

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Long noncoding RNA AC092155 facilitates osteogenic differentiation of adipose-derived stem cells through the miR-143-3p/STMN1 axis.

J Gene Med 2021 May 15:e3363. Epub 2021 May 15.

Department of Orthopedic Surgery, Jinling Hospital, Nanjing University, Nanjing, China.

Background: Numerous studies have demonstrated that long noncoding RNAs (lncRNAs) induce osteogenesis in adipose-derived stem cells (ADSCs). This study aimed to explore the role of lncRNAs AC092155 in promoting osteogenic differentiation of ADSCs.

Methods: MicroRNA (miRNA) and lncRNA sequencing were performed in ADSCs that underwent normal or osteogenic induction. Read More

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Piloting the Feasibility and Preliminary Impact of Adding Birth HIV Polymerase Chain Reaction Testing to the Early Infant Diagnosis Guidelines in Kenya.

Pediatr Infect Dis J 2021 May 7. Epub 2021 May 7.

From the Department of Family Medicine, University of Kansas Medical Center, Kansas City, Missouri Children's Mercy Kansas City, Health Services and Outcomes Research, Kansas City, Missouri School of Medicine, University of Missouri-Kansas City, Kansas City, Missouri Center for Virus Research, Kenya Medical Research Institute, Nairobi, Kenya Department of Preventive Medicine, University of Kansas Medical Center, Kansas City, Missouri Global Health Innovations, Dallas, Texas Global Health Innovations-Kenya, Nairobi, Kenya.

Background: In Kenya, standard early infant diagnosis (EID) with polymerase chain reaction (PCR) testing at 6-week postnatal achieves early treatment initiation (<12 weeks) in <20% of HIV+ infants. Kenya's new early infant diagnosis guidelines tentatively proposed adding PCR testing at birth, pending results from pilot studies.

Methods: We piloted birth testing at 4 Kenyan hospitals between November 2017 and November 2018. Read More

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Core-binding factor beta is required for osteoblast differentiation during fibula fracture healing.

J Orthop Surg Res 2021 May 14;16(1):313. Epub 2021 May 14.

The Second Department of Orthopedics, Shaanxi Traditional Chinese Medicine Hospital, Xi'an, 710003, People's Republic of China.

Background: Growing evidence has implicated core-binding factor beta (Cbfb) as a contributor to osteoblast differentiation, which plays a key role in fracture healing. Herein, we aimed to assess whether Cbfb affects osteoblast differentiation after fibula fracture.

Methods: Initially, we established a Cbfb conditional knockout mouse model for subsequent studies. Read More

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Surface-enhanced Raman spectroscopy for analysis of PCR products of viral RNA of hepatitis C patients.

Spectrochim Acta A Mol Biomol Spectrosc 2021 May 5;259:119908. Epub 2021 May 5.

PCR Laboratory, PINUM Hospital, Faisalabad, Pakistan.

In the current study, for a qualitative and quantitative study of Polymerase Chain Reaction (PCR) products of viral RNA of Hepatitis C virus (HCV) infection, surface-enhanced Raman spectroscopy (SERS) methodology has been developed. SERS was used to identify the spectral features associated with the PCR products of viral RNA of Hepatitis C in various samples of HCV-infected patients with predetermined viral loads. The measurements for SERS were performed on 30 samples of PCR products, which included three PCR products of RNA of healthy individuals, six negative controls, and twenty-one HCV positive samples of varying viral loads (VLs) using Silver nanoparticles (Ag NPs) as a SERS substrates. Read More

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Tracking COVID-19 with wastewater to understand asymptomatic transmission.

Int J Infect Dis 2021 May 11. Epub 2021 May 11.

Department of Microbiology, Faculty of Medicine, Chulalongkorn University, King Chulalongkorn Memorial Hospital, Thai Red Cross Society, Bangkok, Thailand; Antimicrobial Resistance and Stewardship Research Unit, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand. Electronic address:

Introduction: SARS-CoV-2 RNA is excreted in feces of most patients and therefore viral load in wastewater can be used as a surveillance tool to develop an early warning system to help and manage future pandemics.

Methods: We collected wastewater from 24 random locations at Bangkok city center and 26 nearby suburbs from July to December 2020. SARS-CoV-2 RNA copy numbers were measured using real-time polymerase chain reaction (PCR). Read More

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Effect of Exercise Intensity on the Healing of the Bone-Tendon Interface: A Mouse Rotator Cuff Injury Model Study.

Am J Sports Med 2021 May 14:3635465211011751. Epub 2021 May 14.

Department of Sports Medicine & Research Centre of Sports Medicine, Xiangya Hospital, Central South University, Changsha, China.

Background: Injuries at the bone-tendon interface (BTI) are common findings in clinical practice. Rehabilitation procedures after BTI surgery are important but are controversial.

Purpose: To investigate the effects of different exercise intensities on BTI healing by means of an established mouse rotator cuff injury model. Read More

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Assessing standards for prevention of early onset group B streptococcal (GBS) disease in Ireland.

Ir J Med Sci 2021 May 14. Epub 2021 May 14.

Obstetrics and Gynaecology, Rotunda Hospital, Dublin, Ireland.

Background: Early onset group B streptococcal (GBS) disease can cause significant neonatal morbidity and mortality. There is currently no Irish national guideline for GBS screening, and protocols vary across maternity units. Polymerase chain reaction (PCR) testing at induction or labour onset informs triage for antibiotic prophylaxis; however, there are human and infrastructural resource requirements to enable widespread implementation. Read More

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HG-9-91-01 Attenuates Murine Experimental Colitis by Promoting Interleukin-10 Production in Colonic Macrophages Through the SIK/CRTC3 Pathway.

Inflamm Bowel Dis 2021 May 14. Epub 2021 May 14.

State Key Laboratory of Analytical Chemistry for Life Science, School of Life Sciences, Nanjing University, Nanjing, China.

Background: Interleukin-10 (IL-10) is a potent immunoregulatory cytokine that plays a pivotal role in maintaining mucosal immune homeostasis. As a novel synthetic inhibitor of salt-inducible kinases (SIKs), HG-9-91-01 can effectively enhance IL-10 secretion at the cellular level, but its in vivo immunoregulatory effects remain unclear. In this study, we investigated the effects and underlying mechanism of HG-9-91-01 in murine colitis models. Read More

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Systematic application of fluorescence in situ hybridization and immunophenotype profile for the identification of ZNF384 gene rearrangements in B cell acute lymphoblastic leukemia.

Int J Lab Hematol 2021 May 14. Epub 2021 May 14.

Department of Haematology, Christian Medical College, Vellore, India.

Introduction: ZNF384 gene fusions resulting from translocations with several partner genes have been described in B cell acute lymphoblastic leukemia (B-ALL) with a characteristic immunophenotype (aberrant CD13 and or CD33 with dim CD10). The prognosis of patients with this rearrangement appears to depend on the fusion partner. ZNF384 rearrangements have been identified by high through put technologies such as RNA sequencing in most of the studies published. Read More

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CircMKLN1 Suppresses the Progression of Human Retinoblastoma by Modulation of miR-425-5p/PDCD4 Axis.

Curr Eye Res 2021 May 14:1-11. Epub 2021 May 14.

Department of Ophthalmology, Suizhou Hospital, Hubei University of Medicine, Suizhou City, Hubei Province, China.

: Circular RNAs (circRNAs) are essential regulators in tumorigenesis and development. In this study, we focused on the functions of circRNA muskelin 1 (circMKLN1) in retinoblastoma (RB) progression.: Quantitative real-time polymerase chain reaction (qRT-PCR) assay was conducted to determine the levels of circMKLN1, microRNA-425-5p (miR-425-5p) and programmed cell death 4 (PDCD4). Read More

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Our anesthesia experiences in COVID-19 positive patients delivering by cesarean section: A retrospective single-center cohort study.

J Obstet Gynaecol Res 2021 May 13. Epub 2021 May 13.

Department of Anesthesiology and Reanimation, University of Health Sciences Turkey, Bursa Yuksek Ihtisas Training and Research Hospital, Bursa, Turkey.

Aim: Pregnancy increases susceptibility to respiratory complications of viral diseases. This study aims to evaluate our anesthesia practices in pregnant women with COVID-19 undergoing cesarean section.

Methods: A total of 61 patients who underwent cesarean section and had positive Polymerase chain reaction (PCR) testing for COVID-19 with nasopharyngeal swabs were included in the study. Read More

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The Story of a Ship Journey, Malaria, and the Gene IVS-II-745 Mutation: Circassian Immigration to Cyprus.

Glob Med Genet 2021 Jun 16;8(2):69-71. Epub 2021 Mar 16.

Department of Medical Genetics, Faculty of Medicine, Erciyes University, Kayseri, Turkey.

 During 19th century, the Circassians were secluded from their lands and forced to migrate to Ottoman Empire properties. Approximately 2,346 Circassians were exiled from Istanbul to Cyprus Island. During the deportation journey, many of Circassian passed away in consequence of malaria and unknown reasons. Read More

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Individuals Age Determination from Human Dental Pulp Through DNA Analysis by PCR.

Glob Med Genet 2021 Jun 15;8(2):57-61. Epub 2021 Feb 15.

Department of Genetics, Central Research Laboratory, Maratha Mandal's NGH Institute of Dental Sciences and Research Centre, Belagavi, Karnataka, India.

 Age estimation in forensic odontology is having a great importance in recent times because of the request by court or other government authorities so that immigrants whose real age is unknown should not suffer unfair disadvantages because of their supposed age, and so that all legal procedures to which an individual's age is relevant can be properly followed.  The present study was planned to be conducted on pulp tissue and dental hard tissues derived from individuals for DNA isolation and age determination .  The present study was an experimental single-blinded study consisting of 30 extracted teeth categorized into three groups as follows: Group A: 10 to 20 years, Group B: 21 to 30 years, Group C: 31 to 40 years. Read More

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Genetic assessment of BoLA-DRB3 polymorphisms by comparing Bangladesh, Ethiopian, and Korean cattle.

J Anim Sci Technol 2021 Mar 31;63(2):248-261. Epub 2021 Mar 31.

Ethiopian Biotechnology Institute, Addis Ababa 5954, Ethiopia.

Attributable to their major function in pathogen recognition, the use of bovine leukocyte antigens (BoLA) as disease markers in immunological traits in cattle is well established. However, limited report exists on polymorphism of the BoLA gene in zebu cattle breeds by high resolution typing methods. Thus, we used a polymerase chain reaction sequence-based typing (PCR-SBT) method to sequence exon 2 of the BoLA class II DRB3 gene from 100 animals (Boran, n = 13; Sheko, n = 20; Fogera, n = 16; Horro, n = 19), Hanwoo cattle (n = 18) and Bangladesh Red Chittagong zebu (n = 14). Read More

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Plasma Extracellular Vesicle Subtypes May be Useful as Potential Biomarkers of Immune Activation in People With HIV.

Pathog Immun 2021 14;6(1):1-28. Epub 2021 Jan 14.

Centre de recherche du CHU de Québec-Université Laval, Québec, QC, Canada.

Background: Extracellular vesicles (EVs) are intercellular messengers with epigenetic potential since they can shuttle microRNA (miRNA). EVs and miRNA play a role in human immunodeficiency virus (HIV) infection immunopathogenesis. Chronic immune activation and systemic inflammation during HIV infection despite effective antiretroviral therapy (ART) are associated with non-acquired immunodeficiency syndrome (AIDS) comorbidities in people living with HIV (PLWH). Read More

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January 2021

Co-culturing polarized M2 Thp-1-derived macrophages enhance stemness of lung adenocarcinoma A549 cells.

Ann Transl Med 2021 Apr;9(8):709

Department of Respiratory and Critical Care Medicine, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, China.

Background: The tumor microenvironment (TME) is highly associated with cancer stem cells, and affects tumor initiation, progression, and metastasis. This study aimed to explore the underlying molecular mechanism of induction of A549 cancer cell stemness by THP-1-derived macrophages.

Method: The Hedgehog inhibitor (Vismodegib), Notch inhibitor Gamma Secretase Inhibitor (GSI), and Signal Transducer and Activator of Transcription 3 (STAT3) inhibitor Cucurbitacin I (JSI-124) were added separately into the co-culture system of A549 cancer cell with THP-1-derived macrophages. Read More

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Forkhead box O1 targeting replication factor C subunit 2 expression promotes glioma temozolomide resistance and survival.

Ann Transl Med 2021 Apr;9(8):692

Department of Neurology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, China.

Background: Additional mechanisms of temozolomide (TMZ) resistance in gliomas remain uncertain. The aim of this study was to identify another DNA repair mechanism involving forkhead box O1 (FoxO1) and replicator C2 (RFC2) in gliomas.

Methods: We established glioma cells against TMZ, U87R, by exposure to TMZ. Read More

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G-rich sequence factor 1 serves as a prognostic biomarker in septic patients.

Ann Transl Med 2021 Apr;9(8):691

Department of Emergency Medicine, Affiliated Hospital of Nantong University, Nantong, China.

Background: Sepsis is a condition of organ dysfunction caused by infection, and is unavoidably related to costs and mortality; however, no biomarker has yet been identified to clearly predict the prognosis of septic patients. In this study, we aimed to explore the role of guanine-rich sequence factor 1 (GRSF1) in evaluating the severity and prognosis of sepsis.

Methods: The expression of GRSF1 in peripheral blood was measured and analyzed in 42 septic participants and 32 healthy controls respectively by using quantitative reverse transcription polymerase chain reaction (RT-qPCR). Read More

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CD2 T-helper 17-like cells differentiated from a CD133 subpopulation of non-small cell lung carcinoma cells promote the growth of lung carcinoma.

Ann Transl Med 2021 Apr;9(8):687

Institute of Basic Medicine, Hebei Medical University, Shijiazhuang, China.

Background: Cancer stem cells (CSCs) give rise to a diverse variety of differentiated cells, which comprise the bulk of the tumor microenvironment (TME). However, the exact multi-directional differentiation potential of CSCs has not been fully clarified. This study was designed to explore whether CSCs differentiate into cellular components of the TME to promote the growth of lung carcinoma. Read More

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Ferroptosis is involved in the progression of hepatocellular carcinoma through the circ0097009/miR-1261/SLC7A11 axis.

Ann Transl Med 2021 Apr;9(8):675

Department of Minimally Invasive Interventional Radiology, Liver Cancer Study and Service Group, Sun Yat-Sen University Cancer Center, State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Guangzhou, Guangzhou, China.

Background: Circular RNAs (circRNAs) are a class of non-coding RNAs that have been demonstrated to play important roles in tumorigenesis. However, how circRNAs regulate the progression of hepatocellular cancer (HCC) remains unclear.

Methods: In the present study, circRNA microarray analyses were performed with HCC tissues to identify circRNAs that are differentially expressed. Read More

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MiR-140-5p targets Prox1 to regulate the proliferation and differentiation of neural stem cells through the ERK/MAPK signaling pathway.

Ann Transl Med 2021 Apr;9(8):671

Department of Neurology and Institute of Neurology, Ruijin Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.

Background: The expression of miR-140-5p increased in the brain tissue of a bilateral common carotid artery ligation model, while the overexpression of miR-140-5p significantly decreased the number of neurons. The luciferase report experiment in the previous study proved that miR-140-5p negatively regulated one of the potential targets of Prospero-related homeobox 1 (Prox1). Therefore, we want to investigate the effect of miR-140-5p on the proliferation and differentiation of neural stem cells (NSCs) and the underlying mechanism. Read More

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Metastasis-associated gene 1 (MTA1) enhances cisplatin resistance of malignant pleural mesothelioma by ATR-Chk1-mediated DNA repair.

Ann Transl Med 2021 Apr;9(8):670

Department of Oncology of the First Affiliated Hospital of Soochow University, Suzhou, China.

Background: Malignant pleural mesothelioma (MPM) chemoresistance remains a challenge to oncologists. In our previous study, we demonstrated that the aberrant expression of metastasis-associated gene 1 () is associated with carcinogenesis and metastasis in MPM. The aim of the present study was to investigate the mechanism of and chemo-resistance in MPM. Read More

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Clinical and molecular characteristics of isolates at a teaching hospital in Shanghai, China.

Ann Transl Med 2021 Apr;9(8):668

Institute of Antibiotics, Huashan Hospital, Fudan University, Shanghai, China.

Background: (. ) has recently emerged as a cause of life-threatening nosocomial infections in humans. This study aims to investigate the clinical characteristics, homology, and antimicrobial patterns of . Read More

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Therapeutic effects and mechanism of ferulic acid and icariin in mammary gland hyperplasia model rats via regulation of the ERK pathway.

Ann Transl Med 2021 Apr;9(8):666

College of life sciences and Biopharmaceuticals, Shenyang Pharmaceutical University, Shenyang, China.

Background: Ferulic acid (FA) and icariin (ICA), as the active compounds derived from and , respectively, have been shown to promote blood circulation, regulate menstruation, exhibit anti-inflammatory effects, and modulate estrogen levels. The aim of this study was to elucidate the possible mechanism of FA, ICA, and FA-ICA on estrogen-induced mammary gland hyperplasia (MGH) in rats.

Methods: Hematoxylin and eosin (HE) staining was performed to record the pathological changes in breast tissue, and enzyme-linked immunosorbent assay (ELISA) was utilized to determine the serum levels of luteinizing hormone (LH), PRL (prolactin), testosterone (T), estradiol (E2), follicular stimulating hormone (FSH), and progesterone (P). Read More

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LncRNA ABCC6P1 promotes proliferation and migration of papillary thyroid cancer cells via Wnt/β-catenin signaling pathway.

Ann Transl Med 2021 Apr;9(8):664

Department of Endocrinology, The Second Affiliated Hospital of Harbin Medical University, Harbin, China.

Background: LncRNAs play an important regulatory function in the occurrence and progression of papillary thyroid cancer (PTC). This study aimed to investigate the role and mechanism of ATP binding cassette subfamily C member 6 pseudogene 1 (ABCC6P1) in PTC.

Methods: Cancerous and paracancer normal thyroid tissues were collected from 18 patients with PTC, who were operated at the Second Affiliated Hospital of Harbin Medical University. Read More

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Assessment of HCMV-encoded microRNAs in plasma as potential biomarkers in pregnant women with adverse pregnancy outcomes.

Ann Transl Med 2021 Apr;9(8):638

State Key Laboratory of Pharmaceutical Biotechnology, Collaborative Innovation Center of Chemistry for Life Sciences, Jiangsu Engineering Research Center for MicroRNA Biology and Biotechnology, NJU Advanced Institute for Life Sciences (NAILS), School of Life Sciences, Nanjing University, Nanjing, China.

Background: Human cytomegalovirus (HCMV) is the most frequent cause of congenital infections and can lead to adverse pregnancy outcomes (APOs). HCMV encodes multiple microRNAs (miRNAs) that have been reported to be partially related to host immune responses, cell cycle regulation, viral replication, and viral latency, and can be detected in human plasma. However, the relevance for HCMV-encoded miRNAs in maternal plasma as an indicator for APOs has never been evaluated. Read More

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Evaluating the association between single nucleotide polymorphisms in the stonin 2 () gene and keratoconus in a Han Chinese population.

Ann Transl Med 2021 Apr;9(8):616

Eye Institute and Department of Ophthalmology, Eye & ENT Hospital, Fudan University, Shanghai, China.

Background: A recent genome-wide association study (GWAS) identified a significant association between the single nucleotide polymorphism (SNP) rs2371597 in the stonin 2 gene () and keratoconus (KCTN) susceptibility. The current study further explored the association between and KCTN susceptibility in an independent Han Chinese population.

Methods: Three SNPs (rs2371597, rs8004137, and rs8008602) located in the gene were examined in 164 Han Chinese patients with KCTN and 239 age- and gender-matched healthy subjects. Read More

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