79 results match your criteria nes consensus

User redesign, testing and evaluation of the Monitoring Risk and Improving System Safety (MoRISS) checklist for the general practice work environment.

BMJ Open Qual 2020 11;9(4)

Medical Directorate, NHS Education for Scotland West Region, Glasgow, UK.

Background: Inadequate checking of safety-critical issues can compromise care quality in general practice (GP) work settings. Adopting a systemic, methodical approach may lead to improved standardisation of processes and reliability of task performance, strengthening the safety systems concerned. This study aimed to revise, modify and test the content and relevance of a previously validated safety checklist to the current GP context. Read More

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November 2020

Gene correlation network analysis to identify regulatory factors in sepsis.

J Transl Med 2020 10 8;18(1):381. Epub 2020 Oct 8.

Department of Emergency Medicine, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, No 3, East Qingchun Road, Hangzhou, 310016, Zhejiang Province, China.

Background And Objectives: Sepsis is a leading cause of mortality and morbidity in the intensive care unit. Regulatory mechanisms underlying the disease progression and prognosis are largely unknown. The study aimed to identify master regulators of mortality-related modules, providing potential therapeutic target for further translational experiments. Read More

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October 2020

Development and application of 'systems thinking' principles for quality improvement.

BMJ Open Qual 2020 03;9(1)

Medical Directorate, NHS Education for Scotland, Glasgow, UK

Introduction: 'Systems thinking' is often recommended in healthcare to support quality and safety activities but a shared understanding of this concept and purposeful guidance on its application are limited. Healthcare systems have been described as complex where human adaptation to localised circumstances is often necessary to achieve success. Principles for managing and improving system safety developed by the European Organisation for the Safety of Air Navigation (EUROCONTROL; a European intergovernmental air navigation organisation) incorporate a 'Safety-II systems approach' to promote understanding of how safety may be achieved in complex work systems. Read More

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pCRM1exportome: database of predicted CRM1-dependent Nuclear Export Signal (NES) motifs in cancer-related genes.

Bioinformatics 2020 02;36(3):961-963

Department of Biophysics, TX 75390, USA.

Motivation: The consensus pattern of Nuclear Export Signal (NES) is a short sequence motif that is commonly identified in protein sequences, whether the motif acts as an NES (true positive) or not (false positive). Finding more plausible NES functioning regions among the vast array of consensus-matching segments would provide an interesting resource for further experimental validation. Better defined NES should also allow meaningful mapping of cancer-related mutation positions, leading to plausible explanations for the relationship between nuclear export and disease. Read More

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February 2020

The Jembrana disease virus Rev protein: Identification of nuclear and novel lentiviral nucleolar localization and nuclear export signals.

PLoS One 2019 22;14(8):e0221505. Epub 2019 Aug 22.

Département des Sciences Biologiques, Université du Québec à Montréal, Montréal, Québec, Canada.

The lentiviral Rev protein, which is a regulatory protein essential for virus replication, has been first studied in the human immunodeficiency virus type 1 (HIV-1). The main function of Rev is to mediate the nuclear exportation of viral RNAs. To fulfill its function, Rev shuttles between the cytoplasm and the nucleus. Read More

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CD166high Uveal Melanoma Cells Represent a Subpopulation With Enhanced Migratory Capacity.

Invest Ophthalmol Vis Sci 2019 06;60(7):2696-2704

Department of Ophthalmology, Faculty of Medicine, Albert Szent-Gyorgyi Clinical Center, University of Szeged, Szeged, Hungary.

Purpose: Cancer stem cells (CSCs) are a subpopulation of cells with the capacity to drive tumor growth. While there is evidence of the existence of CSCs in uveal melanoma (UM), there is no consensus on their defining markers. In this study, we examined putative CSC markers in UM cell lines, primary UM (PUM), and normal choroidal melanocytes (NCM). Read More

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Modern Training and Credentialing in Neuroendovascular Acute Ischemic Stroke Therapy.

Neurosurgery 2019 07;85(suppl_1):S52-S57

Department of Neurosurgery, University of Tennessee Health Science Center and Semmes-Murphey Clinic, Memphis, Tennessee.

Background: Acute ischemic stroke (AIS) remains a significant source of morbidity and mortality worldwide. Multiple recent randomized clinical trials for AIS patients presenting with large vessel occlusion have demonstrated radical improvement in outcomes with mechanical thrombectomy (MT), which is now recognized as the new standard of care for patients presenting up to 24 hr.

Objective: To provide a perspective on training and credentialing requirements for practitioners in this field. Read More

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Structural prerequisites for CRM1-dependent nuclear export signaling peptides: accessibility, adapting conformation, and the stability at the binding site.

Sci Rep 2019 04 29;9(1):6627. Epub 2019 Apr 29.

Department of Biophysics, University of Texas Southwestern Medical Center, Dallas, TX, 75390, USA.

Nuclear export signal (NES) motifs function as essential regulators of the subcellular location of proteins by interacting with the major nuclear exporter protein, CRM1. Prediction of NES is of great interest in many aspects of research including cancer, but currently available methods, which are mostly based on the sequence-based approaches, have been suffered from high false positive rates since the NES consensus patterns are quite commonly observed in protein sequences. Therefore, finding a feature that can distinguish real NES motifs from false positives is desired to improve the prediction power, but it is quite challenging when only using the sequence. Read More

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Glaucoma management in primary care: barriers perceived by optometrists in Scotland.

Ophthalmic Physiol Opt 2018 11;38(6):629-639

University of Edinburgh, Edinburgh, UK.

Purpose: Over the next 10 years, the prevalence of glaucoma in the United Kingdom (UK) is predicted to rise by 22%,(The Way Forward: Glaucoma, The Royal College of Ophthalmologists, London, 2017) posing a considerable challenge to already overstretched hospital eye services. To help address this problem, services traditionally offered in hospital such as managing stable glaucoma patients, could be transferred to community optometrists. The aim of this study was to identify whether optometrists in Scotland have an interest in managing primary open angle glaucoma (POAG) in primary care and to assess perceived barriers. Read More

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November 2018

Core neurological examination items for neurology clerks: A modified Delphi study with a grass-roots approach.

PLoS One 2018 17;13(5):e0197463. Epub 2018 May 17.

Department of Neurology, Chang Gung Memorial Hospital, Linkou Medical Center, Taoyuan, Taiwan.

Background: With the evolution of treatments for neurological diseases, the contents of core neurological examinations (NEs) for medical students may need to be modified. We aimed to establish a consensus on the core NE items for neurology clerks and compare viewpoints between different groups of panelists.

Methods: First, a pilot group proposed the core contents of NEs for neurology clerks. Read More

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November 2018

NoLogo: a new statistical model highlights the diversity and suggests new classes of Crm1-dependent nuclear export signals.

BMC Bioinformatics 2018 02 27;19(1):65. Epub 2018 Feb 27.

Department of Cell & Systems Biology, University of Toronto, Toronto, Canada.

Background: Crm1-dependent Nuclear Export Signals (NESs) are clusters of alternating hydrophobic and non-hydrophobic amino acid residues between 10 to 15 amino acids in length. NESs were largely thought to follow simple consensus patterns, based on which they were categorized into 6-10 classes. However, newly discovered NESs often deviate from the established consensus patterns. Read More

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February 2018

Prevalence of underweight and small for gestational age in Argentina: Comparison between the INTERGROWTH-21st standard and an Argentine reference

Arch Argent Pediatr 2017 12;115(6):547-555

Instituto de Ecorregiones Andinas (INECOA), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Universidad Nacional de Jujuy (UNJu), Instituto de Biología de la Altura (INBIAL), San Salvador de Jujuy.

Introduction: The term “low birth weight” (< 2500 g) encompasses preterm newborns and term newborns small for gestational age (SGA) (< P10). The World Health Organization de nes underweight as a birth weight < P3 of weight/ age. There is no consensus at an international level about which standards and/or references related to birth weight for gestational age (GA) should be used to assess SGA and underweight among preterm newborns. Read More

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December 2017

Quality of life in inflammatory neuropathies: the IN-QoL.

J Neurol Neurosurg Psychiatry 2018 03 6;89(3):256-262. Epub 2017 Oct 6.

Department of Neurology, Maastricht University Medical Centre, Maastricht, The Netherlands.

Background: No consensus exists which quality of life (QoL) measure should be used in patients with inflammatory neuropathies. Moreover, most QoL measures are ordinal-based scales with their known deficiencies.

Objectives: To establish a new disease-specific interval-based QoL questionnaire in inflammatory neuropathies (IN-QoL) using the Rasch model and evaluate its scientific properties (validity, reliability and responsiveness). Read More

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Nuclear export of misfolded SOD1 mediated by a normally buried NES-like sequence reduces proteotoxicity in the nucleus.

Elife 2017 05 2;6. Epub 2017 May 2.

Center for Biomedical Engineering and Technology, University of Maryland School of Medicine, Baltimore, United States.

Over 170 different mutations in the gene encoding SOD1 all cause amyotrophic lateral sclerosis (ALS). Available studies have been primarily focused on the mechanisms underlying mutant SOD1 cytotoxicity. How cells defend against the cytotoxicity remains largely unknown. Read More

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Night eating syndrome: How to treat it?

Rev Assoc Med Bras (1992) 2016 Oct;62(7):701-707

PhD in Medicine (Pneumology). Associate Professor, Department of Clinical Medicine, UFC, Fortaleza, CE, Brazil.

Night eating syndrome (NES) is characterized by caloric intake ≥ 25% of total daily after dinner and/or by two or more weekly nocturnal awakenings accompanied by food ingestion. Causes of NES are not entirely clear and seem to involve a desynchronization between the circadian rhythms of food ingestion and sleep, resulting in a delayed pattern of food intake. Estimates of the prevalence of NES in the general population are around 1. Read More

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October 2016

NES-REBS: A novel nuclear export signal prediction method using regular expressions and biochemical properties.

J Bioinform Comput Biol 2016 06 7;14(3):1650013. Epub 2016 Apr 7.

* School of Automation, Huazhong University of Science and Technology, Wuhan 430074, Hubei, P. R. China.

A nuclear export signal (NES) is a protein localization signal, which is involved in binding of cargo proteins to nuclear export receptor, thus contributes to regulate localization of cellular proteins. Consensus sequences of NES have been used to detect NES from protein sequences, but suffer from poor predictive power. Some recent peering works were proposed to use biochemical properties of experimental verified NES to refine NES candidates. Read More

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Endocr Pract 2016 Jul 24;22 Suppl 3:1-203. Epub 2016 May 24.

Objective: Development of these guidelines is mandated by the American Association of Clinical Endocrinologists (AACE) Board of Directors and the American College of Endocrinology (ACE) Board of Trustees and adheres to published AACE protocols for the standardized production of clinical practice guidelines (CPGs).

Methods: Recommendations are based on diligent review of clinical evidence with transparent incorporation of subjective factors.

Results: There are 9 broad clinical questions with 123 recommendation numbers that include 160 specific statements (85 [53. Read More

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Clinicopathological and prognostic significance of Nestin expression in patients with non-small cell lung cancer: a systematic review and meta-analysis.

Clin Exp Med 2017 May 21;17(2):161-174. Epub 2016 Apr 21.

Department of Thoracic Surgery, West China Hospital, Sichuan University, Guoxue Alley No. 37, Chengdu, China.

Latest evidence indicates that Nestin expression may be associated with the high malignancy and poor prognosis of non-small cell lung cancer (NSCLC), but a relevant consensus has not been reached until now. Therefore, we conducted this meta-analysis to evaluate the clinicopathological and prognostic significance of Nestin expression in patients with NSCLC. We searched PubMed, EMBASE and the Web of Science for eligible full-text articles. Read More

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Featured Article: Nuclear export of opioid growth factor receptor is CRM1 dependent.

Exp Biol Med (Maywood) 2016 Feb 30;241(3):273-81. Epub 2015 Sep 30.

Department of Neural & Behavioral Sciences, The Pennsylvania State University College of Medicine, Hershey, Pennsylvania 17033, PA, USA

Opioid growth factor receptor (OGFr) facilitates growth inhibition in the presence of its specific ligand opioid growth factor (OGF), chemically termed [Met(5)]-enkephalin. The function of the OGF-OGFr axis requires the receptor to translocate to the nucleus. However, the mechanism of nuclear export of OGFr is unknown. Read More

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February 2016

Structural determinants of nuclear export signal orientation in binding to exportin CRM1.

Elife 2015 Sep 8;4. Epub 2015 Sep 8.

Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, United States.

The Chromosome Region of Maintenance 1 (CRM1) protein mediates nuclear export of hundreds of proteins through recognition of their nuclear export signals (NESs), which are highly variable in sequence and structure. The plasticity of the CRM1-NES interaction is not well understood, as there are many NES sequences that seem incompatible with structures of the NES-bound CRM1 groove. Crystal structures of CRM1 bound to two different NESs with unusual sequences showed the NES peptides binding the CRM1 groove in the opposite orientation (minus) to that of previously studied NESs (plus). Read More

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September 2015

Identification of a nuclear export sequence in the MHC CIITA.

J Immunol 2015 Jun 6;194(12):6102-11. Epub 2015 May 6.

Department of Biology, Sarah Lawrence College, Bronxville, NY 10708

Initiation of an immune response through expression of MHC class II and related genes is under the control of the CIITA. Normally found in both the cytoplasm and nucleus, CIITA is tightly controlled by a variety of posttranslational modifications as well as interactions with other nuclear and cytoplasmic factors, whereas disruption of this dual subcellular localization impairs CIITA functioning and expression of target genes. Although CIITA has well-defined domains necessary for its nuclear import, the region responsible for the translocation of CIITA from the nucleus has not been characterized. Read More

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LocNES: a computational tool for locating classical NESs in CRM1 cargo proteins.

Bioinformatics 2015 May 15;31(9):1357-65. Epub 2014 Dec 15.

Department of Pharmacology, University of Texas Southwestern Medical Center at Dallas, Dallas, TX 75390-9041, USA, Howard Hughes Medical Institute, University of Texas Southwestern Medical Center at Dallas, Dallas, TX 75390-9050, USA, Department of Biophysics and Department of Biochemistry, University of Texas Southwestern Medical Center at Dallas, Dallas, TX 75390-9050, USA Department of Pharmacology, University of Texas Southwestern Medical Center at Dallas, Dallas, TX 75390-9041, USA, Howard Hughes Medical Institute, University of Texas Southwestern Medical Center at Dallas, Dallas, TX 75390-9050, USA, Department of Biophysics and Department of Biochemistry, University of Texas Southwestern Medical Center at Dallas, Dallas, TX 75390-9050, USA.

Motivation: Classical nuclear export signals (NESs) are short cognate peptides that direct proteins out of the nucleus via the CRM1-mediated export pathway. CRM1 regulates the localization of hundreds of macromolecules involved in various cellular functions and diseases. Due to the diverse and complex nature of NESs, reliable prediction of the signal remains a challenge despite several attempts made in the last decade. Read More

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NESmapper: accurate prediction of leucine-rich nuclear export signals using activity-based profiles.

PLoS Comput Biol 2014 Sep 18;10(9):e1003841. Epub 2014 Sep 18.

Kazusa DNA Research Institute, Kisarazu, Chiba, Japan.

The nuclear export of proteins is regulated largely through the exportin/CRM1 pathway, which involves the specific recognition of leucine-rich nuclear export signals (NESs) in the cargo proteins, and modulates nuclear-cytoplasmic protein shuttling by antagonizing the nuclear import activity mediated by importins and the nuclear import signal (NLS). Although the prediction of NESs can help to define proteins that undergo regulated nuclear export, current methods of predicting NESs, including computational tools and consensus-sequence-based searches, have limited accuracy, especially in terms of their specificity. We found that each residue within an NES largely contributes independently and additively to the entire nuclear export activity. Read More

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September 2014

Genetic analysis of the ribosome biogenesis factor Ltv1 of Saccharomyces cerevisiae.

Genetics 2014 Nov 10;198(3):1071-85. Epub 2014 Sep 10.

Biochemistry and Molecular Biology Program, Lewis & Clark College, Portland, Oregon 97219

Ribosome biogenesis has been studied extensively in the yeast Saccharomyces cerevisiae. Yeast Ltv1 is a conserved 40S-associated biogenesis factor that has been proposed to function in small subunit nuclear export. Here we show that Ltv1 has a canonical leucine-rich nuclear export signal (NES) at its extreme C terminus that is both necessary for Crm1 interaction and Ltv1 export. Read More

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November 2014

Comparative analysis of seven viral nuclear export signals (NESs) reveals the crucial role of nuclear export mediated by the third NES consensus sequence of nucleoprotein (NP) in influenza A virus replication.

PLoS One 2014 13;9(8):e105081. Epub 2014 Aug 13.

Viral Infectious Diseases Unit, RIKEN, Wako, Saitama, Japan.

The assembly of influenza virus progeny virions requires machinery that exports viral genomic ribonucleoproteins from the cell nucleus. Currently, seven nuclear export signal (NES) consensus sequences have been identified in different viral proteins, including NS1, NS2, M1, and NP. The present study examined the roles of viral NES consensus sequences and their significance in terms of viral replication and nuclear export. Read More

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December 2015

196th ENMC international workshop: Outcome measures in inflammatory peripheral neuropathies 8-10 February 2013, Naarden, The Netherlands.

Neuromuscul Disord 2013 Nov 5;23(11):924-33. Epub 2013 Jul 5.

Maastricht University Medical Centre, Maastricht, The Netherlands. Electronic address:

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November 2013

The huntingtin N17 domain is a multifunctional CRM1 and Ran-dependent nuclear and cilial export signal.

Hum Mol Genet 2013 Apr 7;22(7):1383-94. Epub 2013 Jan 7.

Department of Biochemistry and Biomedical Sciences, McMaster University, 1200 Main Street West, Hamilton, ON, Canada.

The first 17 amino acids of Huntington's disease (HD) protein, huntingtin, comprise an amphipathic alpha-helical domain that can target huntingtin to the endoplasmic reticulum (ER). N17 is phosphorylated at two serines, shown to be important for disease development in genetic mouse models, and shown to be modified by agents that reverse the disease phenotype in an HD mouse model. Here, we show that the hydrophobic face of N17 comprises a consensus CRM1/exportin-dependent nuclear export signal, and that this nuclear export activity can be affected by serine phospho-mimetic mutants. Read More

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Using simulation to support doctors in difficulty.

Clin Teach 2012 Oct;9(5):285-9

Clincial Skills Centre, University of Dundee, Tayside, UK.

Background: A small percentage of medical trainees will have performance concerns identified within their clinical practice. These trainees require specific interventions to address these concerns. The Postgraduate Ward Simulation Exercise (PgWSE) was developed as a joint collaboration between the University of Dundee and NHS Education for Scotland (NES). Read More

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October 2012

Sequence and structural analyses of nuclear export signals in the NESdb database.

Mol Biol Cell 2012 Sep 25;23(18):3677-93. Epub 2012 Jul 25.

Department of Pharmacology, University of Texas Southwestern Medical Center at Dallas, Dallas, TX 75390, USA.

We compiled >200 nuclear export signal (NES)-containing CRM1 cargoes in a database named NESdb. We analyzed the sequences and three-dimensional structures of natural, experimentally identified NESs and of false-positive NESs that were generated from the database in order to identify properties that might distinguish the two groups of sequences. Analyses of amino acid frequencies, sequence logos, and agreement with existing NES consensus sequences revealed strong preferences for the Φ1-X(3)-Φ2-X(2)-Φ3-X-Φ4 pattern and for negatively charged amino acids in the nonhydrophobic positions of experimentally identified NESs but not of false positives. Read More

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September 2012

Differential splicing alters subcellular localization of the alpha but not beta isoform of the MIER1 transcriptional regulator in breast cancer cells.

PLoS One 2012 24;7(2):e32499. Epub 2012 Feb 24.

Terry Fox Cancer Research Laboratories, Division of BioMedical Sciences, Faculty of Medicine, Memorial University of Newfoundland, St John's, Newfoundland, Canada.

MIER1 was originally identified in a screen for novel fibroblast growth factor activated early response genes. The mier1 gene gives rise to multiple transcripts encoding protein isoforms that differ in their amino (N-) and carboxy (C-) termini. Much of the work to date has focused on the two C-terminal variants, MIER1α and β, both of which have been shown to function as transcriptional repressors. Read More

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