74 results match your criteria napus homologs


Systematic analysis of JmjC gene family and stress--response expression of KDM5 subfamily genes in .

PeerJ 2021 31;9:e11137. Epub 2021 Mar 31.

Key Laboratory of Crop Epigenetic Regulation and Development, Hunan Province, Changsha, China.

Background: Jumonji C (JmjC) proteins exert critical roles in plant development and stress response through the removal of lysine methylation from histones. which originated from spontaneous hybridization by and , is the most important oilseed crop after soybean. In JmjC proteins of species, the structure and function and its relationship with the parents and model plant remain uncharacterized. Read More

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Genome Triplication Leads to Transcriptional Divergence of Genes During Vernalization in the Genus .

Front Plant Sci 2020 9;11:619417. Epub 2021 Feb 9.

Graduate School of Agricultural Science, Kobe University, Kobe, Japan.

The genus includes oil crops, vegetables, condiments, fodder crops, and ornamental plants. species underwent a whole genome triplication event after speciation between ancestral species of and closely related genera including . Diploid species such as and have three copies of genes orthologous to each gene, although deletion in one or two of the three homologs has occurred in some genes. Read More

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February 2021

Regulation and Subfunctionalization of Flowering Time Genes in the Allotetraploid Oil Crop .

Authors:
Sarah Schiessl

Front Plant Sci 2020 20;11:605155. Epub 2020 Nov 20.

Department of Plant Breeding, IFZ Research Centre for Biosystems, Land Use and Nutrition, Justus Liebig University Giessen, Giessen, Germany.

Flowering is a vulnerable, but crucial phase in building crop yield. Proper timing of this period is therefore decisive in obtaining optimal yields. However, genetic regulation of flowering integrates many different environmental signals and is therefore extremely complex. Read More

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November 2020

Genome-wide characterization, expression analyses, and functional prediction of the NPF family in Brassica napus.

BMC Genomics 2020 Dec 7;21(1):871. Epub 2020 Dec 7.

College of Agronomy and Biotechnology, Chongqing Engineering Research Center for Rapeseed, Southwest University, Chongqing, 400716, China.

Background: NITRATE TRANSPORTER 1/PEPTIDE TRANSPORTER (NRT1/PTR) family (NPF) members are essential transporters for many substrates in plants, including nitrate, hormones, peptides, and secondary metabolites. Here, we report the global characterization of NPF in the important oil crop Brassica napus, including that for phylogeny, gene/protein structures, duplications, and expression patterns.

Results: A total of 199 B. Read More

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December 2020

Evolution of the Brassicaceae-specific MS5-Like family and neofunctionalization of the novel MALE STERILITY 5 gene essential for male fertility in Brassica napus.

New Phytol 2021 02 23;229(4):2339-2356. Epub 2020 Nov 23.

Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences/Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan, 430062, China.

New genes (or lineage-specific genes) can facilitate functional innovations. MALE STERILITY 5 (MS5) in Brassica napus is a fertility-related new gene, which has two wild-type alleles (BnMS5 and BnMS5 ) and two mutant alleles (BnMS5 and BnMS5 ) that could induce male sterility. Here, we studied the history and functional evolution of MS5 homologs in plants by phylogenetic analysis and molecular genetic experiments. Read More

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February 2021

A 3-ketoacyl-CoA synthase 11 (KCS11) homolog from Malania oleifera synthesizes nervonic acid in plants rich in 11Z-eicosenoic acid.

Tree Physiol 2021 Feb;41(2):331-342

College of Agronomy, Northwest A&F University, No.3 Taicheng Road Yangling, Yangling, Shaanxi 712100, China.

Nervonic acid (24:1) is a major component in nerve and brain tissues and it has important applications in food and pharmaceutical industries. Malania oleifera seeds contain about 40% nervonic acid. However, the mechanism of nervonic acid biosynthesis and accumulation in seeds of this endangered tree species remains unknown. Read More

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February 2021

A Genome-Wide Survey of MATE Transporters in Brassicaceae and Unveiling Their Expression Profiles under Abiotic Stress in Rapeseed.

Plants (Basel) 2020 Aug 20;9(9). Epub 2020 Aug 20.

Chongqing Engineering Research Center for Rapeseed, College of Agronomy and Biotechnology, Southwest University, No. 2 Tiansheng Road, Beibei, Chongqing 400715, China.

The multidrug and toxic compound extrusion (MATE) protein family is important in the export of toxins and other substrates, but detailed information on this family in the Brassicaceae has not yet been reported compared to . In this study, we identified 57, 124, 81, 85, 130, and 79 genes in , , , , , and , respectively, which were unevenly distributed on chromosomes owing to both tandem and segmental duplication events. Phylogenetic analysis showed that these genes could be classified into four subgroups, shared high similarity and conservation within each group, and have evolved mainly through purifying selection. Read More

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Gene silencing of BnaA09.ZEP and BnaC09.ZEP confers orange color in Brassica napus flowers.

Plant J 2020 11 11;104(4):932-949. Epub 2020 Oct 11.

National Key Laboratory of Crop Genetic Improvement, College of Plant Science and Technology, National Center of Rapeseed Improvement in Wuhan, Huazhong Agricultural University, Wuhan, 430070, China.

Brassica napus is currently cultivated as an important ornamental crop in China. Flower color has attracted much attention in rapeseed genetics and breeding. Here, we characterize an orange-flowered mutant of B. Read More

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November 2020

A copia-like retrotransposon insertion in the upstream region of the SHATTERPROOF1 gene, BnSHP1.A9, is associated with quantitative variation in pod shattering resistance in oilseed rape.

J Exp Bot 2020 09;71(18):5402-5413

Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan Hubei, P.R. China.

Seed loss resulting from pod shattering is a major constraint in production of oilseed rape (Brassica napus L.). However, the molecular mechanisms underlying pod shatter resistance are not well understood. Read More

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September 2020

Global Landscapes of the Family Members Uncover their Potential Roles in Regulating the Rapeseed Resistance to Salt Stress.

Int J Mol Sci 2020 May 12;21(10). Epub 2020 May 12.

School of Agricultural Sciences, Zhengzhou University, Zhengzhou, 450001, China.

Soil salinity is a main abiotic stress in agriculture worldwide. The Na/H antiporters (NHXs) play pivotal roles in intracellular Na excretion and vacuolar Na compartmentalization, which are important for plant salt stress resistance (SSR). However, few systematic analyses of NHXs has been reported in allotetraploid rapeseed so far. Read More

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Genome-Wide Identification and Evolutionary Analysis of the Fruit-Weight 2.2-Like Gene Family in Polyploid Oilseed Rape ( L.).

DNA Cell Biol 2020 May 2;39(5):766-782. Epub 2020 Apr 2.

Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture and Rural Affairs, Wuhan, China.

The fruit-weight 2.2 () locus, which was first described in tomato, is known for controlling up to 30% of fruit mass. The functions of its homologs, the FW2. Read More

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Genome-Wide Analysis of Phosphorus Transporter Genes in and Their Roles in Heavy Metal Stress Tolerance.

Int J Mol Sci 2020 Mar 23;21(6). Epub 2020 Mar 23.

Key Laboratory of Horticulture Science for Southern Mountainous Regions, Ministry of Education, Southwest University, No. 2 Tiansheng Road, Beibei, Chongqing 400715, China.

Phosphorus transporter () genes encode HPO/H co-transporters that absorb and transport inorganic nutrient elements required for plant development and growth and protect plants from heavy metal stress. However, little is known about the roles of in compared to . In this study, we identified and extensively analyzed 336 PHTs from three diploid ( and ) and two allotetraploid ( and ) species. Read More

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Genome-wide identification of the NPR1-like gene family in Brassica napus and functional characterization of BnaNPR1 in resistance to Sclerotinia sclerotiorum.

Plant Cell Rep 2020 Jun 5;39(6):709-722. Epub 2020 Mar 5.

Institute of Life Sciences, Jiangsu University, 301# Xuefu Road, Zhenjiang, 212013, People's Republic of China.

Key Message: The BnaNPR1-like gene family was identified in B. napus, and it was revealed that repression of BnaNPR1 significantly reduces resistance toS. sclerotiorum, intensifies ROS accumulation, and changes the expression of genes associated with SA and JA/ET signaling in response to this pathogen. Read More

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QTL and Candidate Gene Identification for Silique Length Based on High-Dense Genetic Map in L.

Front Plant Sci 2019 29;10:1579. Epub 2019 Nov 29.

Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences/Key Laboratory for Biological Sciences and Genetic Improvement of Oil Crops, Ministry of Agriculture and Rural Affairs, Wuhan, China.

Silique length (SL) is an important yield trait and positively correlates with seeds per silique and seed weight. In the present study, two double haploid (DH) populations, established from crosses Zhongshuang11 × R11 (ZR) and R1 × R2 (RR), containing 280 and 95 DH lines, respectively, were used to map quantitative trait loci (QTL) for SL. A high-dense genetic map from ZR population was constructed comprising 14,658 bins on 19 linkage groups, with map length of 2,198. Read More

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November 2019

Targeted mutagenesis of BnTT8 homologs controls yellow seed coat development for effective oil production in Brassica napus L.

Plant Biotechnol J 2020 05 11;18(5):1153-1168. Epub 2019 Nov 11.

National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, China.

Yellow seed is a desirable trait with great potential for improving seed quality in Brassica crops. Unfortunately, no natural or induced yellow seed germplasms have been found in Brassica napus, an important oil crop, which likely reflects its genome complexity and the difficulty of the simultaneous random mutagenesis of multiple gene copies with functional redundancy. Here, we demonstrate the first application of CRISPR/Cas9 for creating yellow-seeded mutants in rapeseed. Read More

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Different copies of SENSITIVITY TO RED LIGHT REDUCED 1 show strong subfunctionalization in Brassica napus.

BMC Plant Biol 2019 Aug 22;19(1):372. Epub 2019 Aug 22.

RNA Biology and Molecular Physiology, Faculty for Biology, Bielefeld University, Universitaetsstrasse 25, 33615, Bielefeld, Germany.

Background: Correct timing of flowering is critical for plants to produce enough viable offspring. In Arabidopsis thaliana (Arabidopsis), flowering time is regulated by an intricate network of molecular signaling pathways. Arabidopsis srr1-1 mutants lacking SENSITIVITY TO RED LIGHT REDUCED 1 (SRR1) expression flower early, particularly under short day (SD) conditions (1). Read More

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Knockout of two BnaMAX1 homologs by CRISPR/Cas9-targeted mutagenesis improves plant architecture and increases yield in rapeseed (Brassica napus L.).

Plant Biotechnol J 2020 03 13;18(3):644-654. Epub 2019 Aug 13.

Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan, China.

Plant height and branch number are essential components of rapeseed plant architecture and are directly correlated with its yield. Presently, improvement of plant architecture is a major challenge in rapeseed breeding. In this study, we first verified that the two rapeseed BnaMAX1 genes had redundant functions resembling those of Arabidopsis MAX1, which regulates plant height and axillary bud outgrowth. Read More

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Three BnaIAA7 homologs are involved in auxin/brassinosteroid-mediated plant morphogenesis in rapeseed (Brassica napus L.).

Plant Cell Rep 2019 Aug 22;38(8):883-897. Epub 2019 Apr 22.

Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan, 430062, China.

Key Message: BnaIAA7 crosstalk with BR signaling is mediated by the interaction between BnaARF8 and BnaBZR1 to regulate rapeseed plant morphogenesis. Auxin (indole-3-acetic acid, IAA) and brassinosteroids (BRs) are essential regulators of plant morphogenesis. However, their roles in rapeseed have not been reported. Read More

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Association Mapping Analysis of Fatty Acid Content in Different Ecotypic Rapeseed Using mrMLM.

Front Plant Sci 2018 4;9:1872. Epub 2019 Jan 4.

Chongqing Engineering Research Center for Rapeseed, College of Agronomy and Biotechnology, Southwest University, Chongqing, China.

L. is a widely cultivated oil crop and provides important resources of edible vegetable oil, and its quality is determined by fatty acid composition and content. To explain the genetic basis and identify more minor loci for fatty acid content, the multi-locus random-SNP-effect mixed linear model (mrMLM) was used to identify genomic regions associated with fatty acid content in a genetically diverse population of 435 rapeseed accessions, including 77 winter-type, 55 spring-type, and 303 semi-winter-type accessions grown in different environments. Read More

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January 2019

Assigning confidence scores to homoeologs using fuzzy logic.

PeerJ 2019 11;6:e6231. Epub 2019 Jan 11.

SIB Swiss Institute of Bioinformatics, Lausanne, Switzerland.

In polyploid genomes, homoeologs are a specific subtype of homologs, and can be thought of as orthologs between subgenomes. In Orthologous MAtrix, we infer homoeologs in three polyploid plant species: upland cotton (), rapeseed (), and bread wheat (). While we can typically recognize the features of a "good" homoeolog prediction (a consistent evolutionary distance, high synteny, and a one-to-one relationship), none of them is a hard-fast criterion. Read More

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January 2019

Molecular characterization of Brassica napus stress related transcription factors, BnMYB44 and BnVIP1, selected based on comparative analysis of Arabidopsis thaliana and Eutrema salsugineum transcriptomes.

Mol Biol Rep 2018 Oct 23;45(5):1111-1124. Epub 2018 Jul 23.

Institute of Biotechnology, Shiraz University, Shiraz, Iran.

Many studies have been performed to identify regulatory circuit underlying plant stress tolerance. However, the reliability of some findings has been criticized because of exclusive use of stress sensitive plant species such as Arabidopsis thaliana. Sensitive plant species often harbor narrow defensive mechanisms and have relatively low capacity for adaptive responses. Read More

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October 2018

WIND1 induces dynamic metabolomic reprogramming during regeneration in Brassica napus.

Dev Biol 2018 10 17;442(1):40-52. Epub 2018 Jul 17.

RIKEN Center for Sustainable Resource Science, Yokohama 230-0045, Japan. Electronic address:

Plants often display a high competence for regeneration under stress conditions. Signals produced in response to various types of stress serve as critical triggers for de novo organogenesis, but the identity of these signaling molecules underlying cellular reprogramming are largely unknown. We previously identified an AP2/ERF transcription factor, WOUND INDUCED DEDIFFERENTIATION1 (WIND1), as a key regulator involved in wound-induced cellular reprogramming in Arabidopsis. Read More

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October 2018

Whole-transcriptome analysis reveals genetic factors underlying flowering time regulation in rapeseed (Brassica napus L.).

Plant Cell Environ 2018 08 19;41(8):1935-1947. Epub 2018 Jun 19.

Plant Breeding Institute, Christian-Albrechts-University of Kiel, Kiel, Germany.

Rapeseed (Brassica napus L.), one of the most important sources of vegetable oil and protein-rich meals worldwide, is adapted to different geographical regions by modification of flowering time. Rapeseed cultivars have different day length and vernalization requirements, which categorize them into winter, spring, and semiwinter ecotypes. Read More

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Identification and application of exogenous dsRNA confers plant protection against Sclerotinia sclerotiorum and Botrytis cinerea.

Sci Rep 2018 05 9;8(1):7320. Epub 2018 May 9.

Department of Biological Sciences, University of Manitoba, Winnipeg, Manitoba, R3T 2N2, Canada.

Sclerotinia sclerotiorum, the causal agent of white stem rot, is responsible for significant losses in crop yields around the globe. While our understanding of S. sclerotiorum infection is becoming clearer, genetic control of the pathogen has been elusive and effective control of pathogen colonization using traditional broad-spectrum agro-chemical protocols are less effective than desired. Read More

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An Efficient CRISPR/Cas9 Platform for Rapidly Generating Simultaneous Mutagenesis of Multiple Gene Homoeologs in Allotetraploid Oilseed Rape.

Front Plant Sci 2018 20;9:442. Epub 2018 Apr 20.

Oil Crops Research Institute of Chinese Academy of Agricultural Sciences, Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan, China.

With the rapid development of sequence specific nucleases (SSNs) for genome targeting, clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9 (CRISPR/Cas9) is now considered the most promising method for functional genetic researches, as well as genetic improvement in crop plants. However, the gene redundancy feature within the allotetraploid rapeseed genome is one of the major obstacles for simultaneous modification of different homologs in the first generation. In addition, large scale screening to identify mutated transgenic plants is very time-and labor-consuming using the conventional restriction enzyme-based approaches. Read More

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Genome-Wide Identification and Structural Analysis of bZIP Transcription Factor Genes in Brassica napus.

Genes (Basel) 2017 Oct 24;8(10). Epub 2017 Oct 24.

Chongqing Rapeseed Engineering Research Center, College of Agronomy and Biotechnology, Southwest University, Chongqing 400716, China.

The basic region/leucine zipper motif (bZIP) transcription factor family is one of the largest families of transcriptional regulators in plants. bZIP genes have been systematically characterized in some plants, but not in rapeseed (). In this study, we identified 247 genes in the rapeseed genome, which we classified into 10 subfamilies based on phylogenetic analysis of their deduced protein sequences. Read More

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October 2017

Overexpression of in Confers Broad Spectrum Resistance to Fungal Pathogens.

Front Plant Sci 2017 4;8:1693. Epub 2017 Oct 4.

National Research Centre on Plant Biotechnology, New Delhi, India.

(Indian mustard) is a commercially important oil seed crop, which is highly affected by many biotic stresses. Among them, leaf blight and powdery mildew are the most devastating diseases leading to huge yield losses in around the world. In this regard, genetic engineering is a promising tool that may possibly allow us to enhance the disease resistance against these pathogens. Read More

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October 2017

An integrated analysis of QTL mapping and RNA sequencing provides further insights and promising candidates for pod number variation in rapeseed (Brassica napus L.).

BMC Genomics 2017 01 11;18(1):71. Epub 2017 Jan 11.

Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan, 430062, China.

Background: As the most important yield component in rapeseed (Brassica napus L.), pod number is determined by a series of successive growth and development processes. Pod number shows extensive variation in rapeseed natural germplasm, which is valuable for genetic improvement. Read More

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January 2017

Genome-Wide Identification, Localization, and Expression Analysis of Proanthocyanidin-Associated Genes in .

Front Plant Sci 2016 9;7:1831. Epub 2016 Dec 9.

Oilseed Crops Institute, Hunan Agricultural University Changsha, Hunan, China.

Proanthocyanidins (PA) is a type of prominent flavonoid compound deposited in seed coats which controls the pigmentation in all species. Annotation of genome survey sequences showed 72 PA genes; however, a functional description of these genes, especially how their interactions regulate seed pigmentation, remains elusive. In the present study, we designed 19 primer pairs to screen a bacterial artificial chromosome (BAC) library of . Read More

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December 2016

Genome-Wide Identification of Dicer-Like, Argonaute, and RNA-Dependent RNA Polymerase Gene Families in Species and Functional Analyses of Their Arabidopsis Homologs in Resistance to .

Front Plant Sci 2016 27;7:1614. Epub 2016 Oct 27.

Institute of Biotechnology, College of Agriculture and Biotechnology, Zhejiang University Hangzhou, China.

RNA silencing is an important mechanism to regulate gene expression and antiviral defense in plants. Nevertheless, RNA silencing machinery in the important oil crop and function in resistance to the devastating fungal pathogen are not well-understood. In this study, gene families of RNA silencing machinery in were identified and their role in resistance to was revealed. Read More

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October 2016