J Magn Reson 2018 05 1;290:1-11. Epub 2018 Mar 1.
Department of Biomedical Engineering, Columbia University Fu Foundation School of Engineering and Applied Science, 1210 Amsterdam Ave., New York, NY 10027, United States; Department of Radiology and Biomedical Imaging, Yale University School of Medicine, 330 Cedar Street, New Haven, CT 06520, United States; Department of Radiology, Columbia University College of Physicians and Surgeons, 622 W 168 Street, New York, NY 10032, United States; Department of Neurology, Yale University School of Medicine, 330 Cedar Street, New Haven, CT 06520, United States.
Glutathione (GSH) is an endogenous antioxidant implicated in numerous biological processes, including those associated with multiple sclerosis, aging, and cancer. Spectral editing techniques have greatly facilitated the acquisition of glutathione signal in living humans via proton magnetic resonance spectroscopy, but signal quantification at 7 Tesla is still hampered by uncertainty about the glutathione transverse decay rate T relative to those of commonly employed quantitative references like N-acetyl aspartate (NAA), total creatine, or water. While the T of uncoupled singlets can be derived in a straightforward manner from exponential signal decay as a function of echo time, similar estimation of signal decay in GSH is complicated by a spin system that involves both weak and strong J-couplings as well as resonances that overlap those of several other metabolites and macromolecules. Read More