241,056 results match your criteria muscle cells

Multi-scale structuring of cell-instructive cellulose nanocrystal composite hydrogel sheets via sequential electrospinning and thermal wrinkling.

Acta Biomater 2021 May 1. Epub 2021 May 1.

Department of Chemical Engineering, McMaster University, 1280 Main Street West, Hamilton, ON L8S 4L8, Canada; School of Biomedical Engineering, McMaster University, 1280 Main Street West, Hamilton, ON L8S 4L8, Canada. Electronic address:

Structured hydrogel sheets offer the potential to mimic the mechanics and morphology of native cell environments in vitro; however, controlling the morphology of such sheets across multiple length scales to give cells consistent multi-dimensional cues remains challenging. Here, we demonstrate a simple two-step process based on sequential electrospinning and thermal wrinkling to create nanocomposite poly(oligoethylene glycol methacrylate)/cellulose nanocrystal hydrogel sheets with a highly tunable multi-scale wrinkled (micro) and fibrous (nano) morphology. By varying the time of electrospinning, rotation speed of the collector, and geometry of the thermal wrinkling process, the hydrogel nanofiber density, fiber alignment, and wrinkle geometry (biaxial or uniaxial) can be independently controlled. Read More

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PLD1 promotes tumor invasion by regulation of MMP-13 expression via NF-κB signaling in bladder cancer.

Cancer Lett 2021 May 1. Epub 2021 May 1.

Department of Urology, Faculty of Medicine and Graduate School of Comprehensive Human Science, University of Tsukuba, Ibaraki, Japan.

Invasion of bladder cancer (BC) cells from the mucosa into the muscle layer is canonical for BC progression while phospholipase D isoform 1 (PLD1) is known to mediate development of cancer through phosphatidic acid (PA) production. We therefore used in silico, in vitro and in vivo approaches to detail the effect of PLD1 on BC invasion. In BC patients, higher levels of PLD1 expression were associated with poor prognoses. Read More

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Targeting microRNA-mediated gene repression limits adipogenic conversion of skeletal muscle mesenchymal stromal cells.

Cell Stem Cell 2021 Apr 27. Epub 2021 Apr 27.

Department of Neurology and Neurological Sciences, Stanford University School of Medicine, Stanford, CA 94305, USA; Paul F. Glenn Center for the Biology of Aging, Stanford University School of Medicine, Stanford, CA 94305, USA; Center for Tissue Regeneration, Repair and Restoration, Veterans Affairs Palo Alto Health Care System, Palo Alto, CA 94304, USA. Electronic address:

Intramuscular fatty deposits, which are seen in muscular dystrophies and with aging, negatively affect muscle function. The cells of origin of adipocytes constituting these fatty deposits are mesenchymal stromal cells, fibroadipogenic progenitors (FAPs). We uncover a molecular fate switch, involving miR-206 and the transcription factor Runx1, that controls FAP differentiation to adipocytes. Read More

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miR-25-5p regulates endothelial progenitor cell differentiation in response to shear stress through targeting ABCA1.

Cell Biol Int 2021 May 4. Epub 2021 May 4.

Department of Neurosurgery, Changhai Hospital of Shanghai affiliated to Naval Military Medical University, Shanghai, 200433, China.

The importance of flow shear stress (SS) on the differentiation of endothelial progenitor cells (EPCs) has been demonstrated in various studies. Cholesterol retention and miRNA regulation have been also proposed as relevant factors involved in this process, though evidence regarding their regulatory roles in the differentiation of EPCs is currently lacking. In the present study on high shear stress (HSS)-induced differentiation of EPCs, we investigated the importance of ABCA1, an important regulator in cholesterol efflux, and miR-25-5p, a potential regulator of endothelial reconstruction. Read More

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Calcium-Activated K Channels (KCa) and Therapeutic Implications.

Handb Exp Pharmacol 2021 May 5. Epub 2021 May 5.

Smooth Muscle Research Centre, Dundalk Institute of Technology, Dundalk, Ireland.

Potassium channels are the most diverse and ubiquitous family of ion channels found in cells. The Ca and voltage gated members form a subfamily that play a variety of roles in both excitable and non-excitable cells and are further classified on the basis of their single channel conductance to form the small conductance (SK), intermediate conductance (IK) and big conductance (BK) K channels.In this chapter, we will focus on the mechanisms underlying the gating of BK channels, whose function is modified in different tissues by different splice variants as well as the expanding array of regulatory accessory subunits including β, γ and LINGO subunits. Read More

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Severe rhabdomyolysis induced by co-administration of cocaine and heroin in a 45 years old man treated with rosuvastatin: a case report.

Acta Biomed 2021 Apr 30;92(S1):e2021089. Epub 2021 Apr 30.

Academy of Emergency Medicine and Care (AcEMC), Pavia, Italy.

The term rhabdomyolysis describes a damage involving striated muscle cells or fibers, often complicated by acute kidney injury. This syndrome can have different causes, but it is generally divided into two main categories: traumatic and non-traumatic rhabdomyolysis. Among medical causes, drugs and abuse substances play a pivotal role, being opioids, alcohol, cocaine and other substances of abuse. Read More

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Cisatracurium inhibits the growth and induces apoptosis of ovarian cancer cells by promoting lincRNA-p21.

Bioengineered 2021 Dec;12(1):1505-1516

Department of Anesthesiology, Affiliated Hospital of Qingdao University, Qingdao, Shandong, China.

As a common muscle relaxant, cisatracurium has shown good antitumor effect on some tumors. Recent studies reported that cisatracurium could inhibit the progression of colon cancer by upregulating tumor suppressor gene p53. However, its role in ovarian cancer and its regulatory effect on p53 and p53 downstream targeting gene long intergenic noncoding RNA p21 (lincRNA-p21) is still unknown. Read More

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December 2021

GGC repeat expansions in NOTCH2NLC causing a phenotype of distal motor neuropathy and myopathy.

Ann Clin Transl Neurol 2021 May 4. Epub 2021 May 4.

Department of Neurology, Peking University First Hospital, Beijing, 100034, China.

Background: The expansion of GGC repeat in the 5' untranslated region of the NOTCH2NLC has been associated with various neurogenerative disorders of the central nervous system and, more recently, oculopharyngodistal myopathy. This study aimed to report patients with distal weakness with both neuropathic and myopathic features on electrophysiology and pathology who present GGC repeat expansions in the NOTCH2NLC.

Methods: Whole-exome sequencing (WES) and long-read sequencing were implemented to identify the candidate genes. Read More

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A novel lncRNA promotes myogenesis of bovine skeletal muscle satellite cells via PFN1-RhoA/Rac1.

J Cell Mol Med 2021 May 4. Epub 2021 May 4.

Tianjin Key Laboratory of Agricultural Animal Breeding and Healthy Husbandry, College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin, China.

Myogenesis, the process of skeletal muscle formation, is a highly coordinated multistep biological process. Accumulating evidence suggests that long non-coding RNAs (lncRNAs) are emerging as a gatekeeper in myogenesis. Up to now, most studies on muscle development-related lncRNAs are mainly focussed on humans and mice. Read More

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Reduced purine biosynthesis in humans after their divergence from Neandertals.

Elife 2021 May 4;10. Epub 2021 May 4.

Evolutionary Genetics, Max Planck Institute for Evolutionary Anthropology, Leipzig, Germany.

We analyze the metabolomes of humans, chimpanzees and macaques in muscle, kidney and three different regions of the brain. Whereas several compounds in amino acid metabolism occur at either higher or lower concentrations in humans than in the other primates, metabolites downstream of adenylosuccinate lyase, which catalyzes two reactions in purine synthesis, occur at lower concentrations in humans. This enzyme carries an amino acid substitution that is present in all humans today but absent in Neandertals. Read More

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Interocular Suppression in Primary Visual Cortex in Strabismus.

J Neurosci 2021 May 3. Epub 2021 May 3.

Program in Neuroscience, Department of Ophthalmology, University of California, San Francisco, San Francisco, CA 94143 USA

People with strabismus acquired during childhood do not experience diplopia (double vision). To investigate how perception of the duplicate image is suppressed, we raised two male monkeys with alternating exotropia by disinserting the medial rectus muscle in each eye at age 4 weeks. Once the animals were mature, they were brought to the laboratory and trained to fixate a small spot while recordings were made in V1. Read More

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Human muscle production in vitro from pluripotent stem cells: Basic and clinical applications.

Semin Cell Dev Biol 2021 Apr 30. Epub 2021 Apr 30.

Department of Pathology, Brigham and Women's Hospital, Boston, MA, USA; Department of Genetics, Harvard Medical School, Boston, MA, USA; Harvard Stem Cell Institute, Boston, MA, USA. Electronic address:

Human pluripotent stem cells (PSCs), which have the capacity to self-renew and differentiate into multiple cell types, offer tremendous therapeutic potential and invaluable flexibility as research tools. Recently, remarkable progress has been made in directing myogenic differentiation of human PSCs. The differentiation strategies, which were inspired by our knowledge of myogenesis in vivo, have provided an important platform for the study of human muscle development and modeling of muscular diseases, as well as a promising source of cells for cell therapy to treat muscular dystrophies. Read More

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Visceral-to-subcutaneous fat ratio independently predicts the prognosis of locally advanced gastric cancer----- highlighting the role of adiponectin receptors and PPARα, β/ δ, ɤ.

Eur J Surg Oncol 2021 Apr 26. Epub 2021 Apr 26.

Department of Surgery, Chang Gung Memorial Hospital, Linkou & Chang Gung University, Taiwan. Electronic address:

Background: Results of computed tomography body composition (CTBC) predicting long-term outcomes of gastric cancer have been mixed and the plausible mechanism remains elusive.

Methods: We retrospectively enrolled a cohort of stage III gastric cancer who had undergone curative-intent gastrectomy. Clinicopathological variables, preoperative CTBC including abdominal muscle, subcutaneous adipose tissue (SAT) and visceral adipose tissue (VAT), and nutritional and inflammatory index were taken together to construct prognostic analysis. Read More

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S-nitrosothiols, and other products of nitrate metabolism, are increased in multiple human blood compartments following ingestion of beetroot juice.

Redox Biol 2021 Apr 16;43:101974. Epub 2021 Apr 16.

University of Exeter Medical School, College of Medicine and Health, St. Luke's Campus, University of Exeter, Heavitree Road, Exeter, EX1 2LU, UK. Electronic address:

Ingested inorganic nitrate (NO⁻) has multiple effects in the human body including vasodilation, inhibition of platelet aggregation, and improved skeletal muscle function. The functional effects of oral NO⁻ involve the in vivo reduction of NO⁻ to nitrite (NO⁻) and thence to nitric oxide (NO). However, the potential involvement of S-nitrosothiol (RSNO) formation is unclear. Read More

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The cytokine interleukin-11 crucially links bone formation, remodeling and resorption.

Cytokine Growth Factor Rev 2021 Apr 24;60:18-27. Epub 2021 Apr 24.

Department of Pathology, Otto-von-Guericke-University Magdeburg, Medical Faculty, Magdeburg, Germany. Electronic address:

Bone development is a complex process that requires the activity of several different signaling pathways and cell types. It involves the coordinated action of osteoclasts (cells that are capable of resorbing bone), osteoblasts (cells that are able to form bone), osteocytes (cells that form a syncytial network within the bone), skeletal muscle cells and the bone marrow. In recent years, the cytokine interleukin-11 (IL-11), a member of the IL-6 family of cytokines, has emerged as an important regulatory protein for bone formation, remodeling and resorption. Read More

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RS4651 suppresses lung fibroblast activation via the TGF-β1/SMAD signalling pathway.

Eur J Pharmacol 2021 Apr 30:174135. Epub 2021 Apr 30.

Department of Infectious Disease, Beijing Friendship Hospital, Capital Medical University, Beijing, 100050, China. Electronic address:

Background: Idiopathic pulmonary fibrosis (IPF) is a progressive disease resulting in respiratory failure with no efficient treatment options. We investigated the protective effect of RS4651 on pulmonary fibrosis in mice and the mechanism.

Methods: Intratracheal injection of bleomycin (BLM) was used to induce pulmonary fibrosis in mice. Read More

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C-bouton components on rat extensor digitorum longus motoneurons are resistant to chronic functional overload.

J Anat 2021 May 3. Epub 2021 May 3.

Department of Neuromuscular Diseases, UCL Queen Square Institute of Neurology, University College London, London, UK.

Mammalian motor systems adapt to the demands of their environment. For example, muscle fibre types change in response to increased load or endurance demands. However, for adaptations to be effective, motoneurons must adapt such that their properties match those of the innervated muscle fibres. Read More

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Surgical resection of extremely rare primary giant splenic angiomyolipoma: a case report.

Surg Case Rep 2021 May 3;7(1):110. Epub 2021 May 3.

Department of Surgery, Tohoku Rosai Hospital, 4-3-21, Dainohara, Aobaku, Sendai, Miyagi, 981-8563, Japan.

Background: Angiomyolipoma is a benign mesenchymal tumor that develops commonly in the kidney and rarely in other organs. The involvement of the spleen in angiomyolipoma is extremely rare, and only one such case has been reported in the English literature.

Case Presentation: A 27-year-old man presented with adenoid hyperplasia and bilateral palatal tonsillar hyperplasia. Read More

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Isolation and Characterization of Human Adipocyte-Derived Extracellular Vesicles using Filtration and Ultracentrifugation.

J Vis Exp 2021 Apr 19(170). Epub 2021 Apr 19.

Division of Cardiovascular Medicine, Radcliffe Department of Medicine, University of Oxford.

Extracellular vesicles (EVs) are lipid enclosed envelopes that carry biologically active material such as proteins, RNA, metabolites and lipids. EVs can modulate the cellular status of other cells locally in tissue microenvironments or through liberation into peripheral blood. Adipocyte-derived EVs are elevated in the peripheral blood and show alterations in their cargo (RNA and protein) during metabolic disturbances, including obesity and diabetes. Read More

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Dihydroartemisinin ameliorates balloon injury-induced neointimal formation through suppressing autophagy in vascular smooth muscle cells.

Biol Chem 2021 Mar 9;402(4):451-460. Epub 2020 Dec 9.

Department of General Surgery, The First Affiliated Hospital of Harbin Medical University, Harbin150001, Heilongjiang, China.

The present study was designed to investigate the therapeutic effects of injection of dihydroartemisinin (DHA) into the balloon-injured carotid arteries on balloon injury-induced neointimal formation and to explore whether autophagy is involved in the action of DHA. Percutaneous transluminal balloon angioplasty was performed in Sprague-Dawley rats to induce neointimal formation, immediately after which DHA (100 μmol/l × 1 ml) and/or Rapamycin (1 mg/100 μl), were injected into the balloon-injured carotid arteries. After 14 days, the serum samples and carotid artery tissues were harvested for analysis. Read More

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Light-Triggered In Situ Gelation of Hydrogels using 2D Molybdenum Disulfide (MoS ) Nanoassemblies as Crosslink Epicenter.

Adv Mater 2021 May 3:e2101238. Epub 2021 May 3.

Biomedical Engineering, College of Engineering, Texas A&M University, College Station, TX, 77843, USA.

Light-responsive biomaterials are an emerging class of materials used for developing noninvasive, noncontact, precise, and controllable biomedical devices. Long-wavelength near-infrared (NIR) radiation is an attractive light source for in situ gelation due to its higher penetration depth and minimum side effects. The conventional approach to obtain crosslinked biomaterials relies heavily on the use of a photoinitiator by generating reactive species when exposed to short-wavelength radiation, which is detrimental to surrounding cells and tissue. Read More

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Can a Peritoneal Conduit Become an Artery?

EJVES Vasc Forum 2020 13;49:23-29. Epub 2020 Oct 13.

Institute for Circulation and Imaging (ISB) at the Norwegian University of Science and Technology (NTNU), Trondheim, Norway.

Objective: Current vascular grafts all have limitations. This study examined peritoneum as a potential graft material and the transfer of peritoneum into a functional artery like conduit after end to end anastomosis into the common carotid artery of sheep. The aim was to investigate whether implantation of a peritoneal tube into the arterial tree results in a structure with function, histological findings, and gene expression like an artery, and whether such arterialisation occurs through a conversion of the phenotype of peritoneal cells or from host cell migration into the implant. Read More

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October 2020

Protocol for RNA-seq library preparation starting from a rare muscle stem cell population or a limited number of mouse embryonic stem cells.

STAR Protoc 2021 Jun 15;2(2):100451. Epub 2021 Apr 15.

Laboratory of Muscle Stem Cells and Gene Regulation, NIAMS, NIH, Bethesda, MD 20892, USA.

It remains challenging to generate reproducible, high-quality cDNA libraries from RNA derived from rare cell populations. Here, we describe a protocol for high-throughput RNA-seq library preparation, including isolation of 200 skeletal muscle stem cells from mouse tibialis anterior muscle by fluorescence-activated cell sorting and cDNA preparation. We also describe RNA extraction and cDNA preparation from differentiating mouse embryonic stem cells. Read More

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Self-curling electroconductive nerve dressing for enhancing peripheral nerve regeneration in diabetic rats.

Bioact Mater 2021 Nov 14;6(11):3892-3903. Epub 2021 Apr 14.

Department of Spine Surgery, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, 510630, China.

Conductive scaffolds have been shown to exert a therapeutic effect on patients suffering from peripheral nerve injuries (PNIs). However, conventional conductive conduits are made of rigid structures and have limited applications for impaired diabetic patients due to their mechanical mismatch with neural tissues and poor plasticity. We propose the development of biocompatible electroconductive hydrogels (ECHs) that are identical to a surgical dressing in this study. Read More

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November 2021

Case Report: Multiple Cavernous Pericardial Lymphangioma (Pericardial Lymphangiomatosis) in a Captive Peregrine Falcon ().

Front Vet Sci 2021 14;8:662157. Epub 2021 Apr 14.

Departamento de Sanidad Animal, Facultad de Veterinaria, Instituto de Ganadería de Montaña (CSIC-ULE), Universidad de León, León, Spain.

A 12-year-old female peregrine falcon () from a private raptor breeding facility that presented a good body condition, died suddenly without showing previous symptoms. At necropsy, in the coelomic cavity, multiple cystic structures demarcated by a thin transparent wall and filled with a serous content were observed. They were firmly adhered to the cranial part of the epicardium and adjacent tissues and occupied the entire thoracic area of the coelomic cavity. Read More

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Layer-By-Layer Fabrication of Large and Thick Human Cardiac Muscle Patch Constructs With Superior Electrophysiological Properties.

Front Cell Dev Biol 2021 16;9:670504. Epub 2021 Apr 16.

Department of Biomedical Engineering, School of Medicine and School of Engineering, University of Alabama at Birmingham, Birmingham, AL, United States.

Engineered cardiac tissues fabricated from human induced pluripotent stem cells (hiPSCs) show promise for ameliorating damage from myocardial infarction, while also restoring function to the damaged left ventricular (LV) myocardium. For these constructs to reach their clinical potential, they need to be of a clinically relevant volume and thickness, and capable of generating synchronous and forceful contraction to assist the pumping action of the recipient heart. Design prerequisites include a structure thickness sufficient to produce a beneficial contractile force, prevascularization to overcome diffusion limitations and sufficient structural development to allow for maximal cell communication. Read More

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ORAI1 Ca Channel as a Therapeutic Target in Pathological Vascular Remodelling.

Front Cell Dev Biol 2021 6;9:653812. Epub 2021 Apr 6.

School of Medicine, The Leeds Institute of Cardiovascular and Metabolic Medicine, University of Leeds, Leeds, United Kingdom.

In the adult, vascular smooth muscle cells (VSMC) are normally physiologically quiescent, arranged circumferentially in one or more layers within blood vessel walls. Remodelling of native VSMC to a proliferative state for vascular development, adaptation or repair is driven by platelet-derived growth factor (PDGF). A key effector downstream of PDGF receptors is store-operated calcium entry (SOCE) mediated through the plasma membrane calcium ion channel, ORAI1, which is activated by the endoplasmic reticulum (ER) calcium store sensor, stromal interaction molecule-1 (STIM1). Read More

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Targeting the Urotensin II/UT G Protein-Coupled Receptor to Counteract Angiogenesis and Mesenchymal Hypoxia/Necrosis in Glioblastoma.

Front Cell Dev Biol 2021 14;9:652544. Epub 2021 Apr 14.

UNIROUEN, INSERM U1239, DC2N, Institute for Research and Innovation in Biomedicine (IRIB), Normandie Rouen Université, Rouen, France.

Glioblastomas (GBMs) are the most common primary brain tumors characterized by strong invasiveness and angiogenesis. GBM cells and microenvironment secrete angiogenic factors and also express chemoattractant G protein-coupled receptors (GPCRs) to their advantage. We investigated the role of the vasoactive peptide urotensin II (UII) and its receptor UT on GBM angiogenesis and tested potential ligand/therapeutic options based on this system. Read More

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Foreign Body Reaction to Implanted Biomaterials and Its Impact in Nerve Neuroprosthetics.

Front Bioeng Biotechnol 2021 15;9:622524. Epub 2021 Apr 15.

Electrical Engineering Division, Department of Engineering, University of Cambridge, Cambridge, United Kingdom.

The implantation of any foreign material into the body leads to the development of an inflammatory and fibrotic process-the foreign body reaction (FBR). Upon implantation into a tissue, cells of the immune system become attracted to the foreign material and attempt to degrade it. If this degradation fails, fibroblasts envelop the material and form a physical barrier to isolate it from the rest of the body. Read More

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Segmental arterial mediolysis of the middle colic artery: report of a case with special reference to lesions of small arteries and veins.

Int J Clin Exp Pathol 2021 15;14(4):508-513. Epub 2021 Apr 15.

Department of Pathology, Amagasaki Central Hospital Hyogo, Japan.

We report a case of segmental arterial mediolysis (SAM) that involved the middle colic artery, and present some pathologic alterations found in mesenteric small arteries and veins. The patient, a 52-year-old woman, underwent an emergency laparotomy for acute intra-abdominal hemorrhage, and a segment of the transverse colon with hemorrhagic mesocolon was excised. Microscopic examination demonstrated two separate lesions of SAM in the middle colic artery. Read More

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