15,112 results match your criteria murine colorectal

Diosgenin exerts anti-tumor effects through inactivation of cAMP/PKA/CREB signaling pathway in colorectal cancer.

Eur J Pharmacol 2021 Jul 26:174370. Epub 2021 Jul 26.

Xiamen Cell Therapy Research Center, The First Affiliated Hospital of Xiamen University, School of Medicine, Xiamen University, Xiamen, 361003, Fujian Province, China. Electronic address:

Colorectal cancer (CRC) is the most fatal gastrointestinal tumor and it is urge to explore powerful drugs for the treatment. Diosgenin (DSG) as a new steroidal had been reported exerts anti-tumor activity in multiple cancers, including CRC. However, the potential mechanism of DSG suppresses CRC remains further to be revealed. Read More

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ILT4 in Colorectal Cancer Cells Induces Suppressive T Cell Contexture and Disease Progression.

Onco Targets Ther 2021 20;14:4239-4254. Epub 2021 Jul 20.

Department of Pathology, Jinan Central Hospital, Cheeloo College of Medicine, Shandong University, Jinan, Shandong, 250013, People's Republic of China.

Purpose: Immune checkpoint blockade (ICB) therapy shows little or no clinical benefit in most colorectal cancer (CRC) patients, due to the immunosuppressive T cell contexture in the tumor microenvironment (TME). Immunoglobulin-like transcript (ILT) 4 is an immunosuppressive molecule in myeloid cells. ILT4 is enriched in solid tumor cells, facilitating their proliferation, invasion, and metastasis. Read More

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PARP-Targeted Auger Therapy in p53 Mutant Colon Cancer Xenograft Mouse Models.

Mol Pharm 2021 Jul 28. Epub 2021 Jul 28.

Department of Biological Sciences Hunter College, City University of New York, New York, New York 10065, United States.

Despite Auger electrons being highly appealing due to their short-range and high linear energy transfer to surrounding tissues, the progress in the field has been limited due to the challenge in delivering a therapeutic dose within the close proximity of cancer cell's DNA. Here, we demonstrate that the PARP inhibitor I-MAPi is a viable agent for the systemic administration and treatment of p53 mutant cancers. Significantly, minimal off-site toxicity was observed in mice administered with up to 74 MBq of I-PARPi. Read More

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Induction of colorectal carcinogenesis in the C57BL/6J and A/J mouse strains with a reduced DSS dose in the AOM/DSS model.

Lab Anim Res 2021 Jul 27;37(1):19. Epub 2021 Jul 27.

Department of Preclinical Sciences and Pathology, Faculty of Veterinary Medicine, Norwegian University of Life Sciences (NMBU), Oslo, Norway.

Background: Colorectal cancer (CRC) is one of the most frequently diagnosed cancers worldwide and thus mouse models of CRC are of significant value to study the pathogenesis. The Azoxymethane/Dextran sulfate sodium (AOM/DSS) model is a widely used, robust initiation-promotion model for chemical induction of colitis-associated CRC in rodents. However, the dosage of chemicals, treatment regimens and outcome measures vary greatly among studies employing this model. Read More

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Ibudilast suppresses oxaliplatin-induced mechanical allodynia and neurodegeneration in rats.

J Pharmacol Sci 2021 Sep 12;147(1):114-117. Epub 2021 Jun 12.

Department of Neuropharmacology, Faculty of Pharmaceutical Sciences, Fukuoka University, Fukuoka, 814-0180, Japan; A.I.G. Collaborative Research Institute for Aging and Brain Sciences, Fukuoka University, Fukuoka, 814-0180, Japan.

Oxaliplatin is a key drug used in the management of solid tumors, such as colorectal cancer; however, it causes peripheral neuropathy. In this study, we investigated the effect of ibudilast, a phosphodiesterase inhibitor, on oxaliplatin-induced mechanical allodynia and histological changes in rats. Ibudilast (7. Read More

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September 2021

Combined mechanical and oral antibiotic bowel preparation is associated with prolonged recurrence-free survival following surgery for colorectal cancer.

J Surg Oncol 2021 Jul 26. Epub 2021 Jul 26.

Division of Colon and Rectal Surgery, University of Chicago, Chicago, Illinois, USA.

Background And Objectives: Recent studies suggest that bacteria influence the pathogenesis of primary colorectal cancer (CRC), yet their role in recurrence after resection is largely unknown. We have discovered that collagenase-producing bacteria promote cancer recurrence in mice, and that antibiotic bowel decontamination decreases colonization of these same organisms in humans. We hypothesized that preoperative combined mechanical and oral antibiotic bowel preparation would improve disease-free survival (DFS) in patients undergoing surgery for CRC. Read More

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IGF2BP2 promotes the progression of colorectal cancer through a YAP-dependent mechanism.

Cancer Sci 2021 Jul 26. Epub 2021 Jul 26.

Department of Laboratory Medicine, Shanghai Tongji Hospital, Tongji University School of Medicine, Shanghai, 200065, China.

Background/aims: Insulin-like growth factor 2 (IGF2) mRNA-binding protein 2 (IGF2BP2) is a post-transcriptional regulatory factor that has been implicated in the development of cancer. Herein, this study was performed to examine the possible role of of IGF2BP2 in colorectal cancer (CRC) and its underlying mechanism.

Methods: First, the expression patterns of IGF2BP2, YAP, and ErbB2 were detected in clinical tissue samples from CRC patients and CRC cell lines. Read More

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Deletion of mTOR in liver epithelial cells enhances hepatic metastasis of colon cancer.

J Pathol 2021 Jul 26. Epub 2021 Jul 26.

Department of General, Visceral- and Transplantation Surgery, RWTH University Hospital, Pauwelsstraße 30, 52074, Aachen, Germany.

Activation of the mTOR pathway is frequently found in cancer, but mTOR inhibitors have thus far failed to demonstrate significant antiproliferative efficacy in the majority of cancer types. Besides cancer cell-intrinsic resistance mechanisms, it is conceivable that mTOR inhibitors impact on non-malignant host cells in a manner that ultimately supports resistance of cancer cells. Against this background, we sought to analyze the functional consequences of mTOR inhibition in hepatocytes for the growth of metastatic colon cancer. Read More

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[Parathyroid hormone-related protein aggravates nonalcoholic fatty liver disease induced by methionine choline-deficient diet in mice].

B Qin N Diao L Bai

Nan Fang Yi Ke Da Xue Xue Bao 2021 Jul;41(7):1037-1043

Guangdong Provincial Key Laboratory of Gastroenterology, Department of Gastroenterology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.

Objective: To study the effect of parathyroid hormone-related protein (PTHrP) on nonalcoholic fatty liver disease (NAFLD) induced by methionine choline-deficient diet (MCD) in mice.

Methods: Twelve male C57BL/6J mice were randomized into blank control group, vehicle group and PTHrP group (=4). The mice in vehicle group and PTHrP group received injections of a control adeno-associated virus (AAV) vector and an AVV vector carrying PTHrP (AAV-PTHrP) gene, respectively, followed one week later by MCD feeding for 3 weeks; the mice in the blank control were fed a normal diet for 4 weeks. Read More

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Combination Foretinib and Anti-PD-1 Antibody Immunotherapy for Colorectal Carcinoma.

Front Cell Dev Biol 2021 8;9:689727. Epub 2021 Jul 8.

State Key Laboratory of Biotherapy and Cancer Center/Collaborative Innovation Center for Biotherapy, West China Hospital, Sichuan University, Chengdu, China.

Immune checkpoint inhibitors have achieved unprecedented success in cancer immunotherapy. However, the overall response rate to immune checkpoint inhibitor therapy for many cancers is only between 20 and 40%, and even less for colorectal cancer (CRC) patients. Thus, there is an urgent need to develop an efficient immunotherapeutic strategy for CRC. Read More

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3-D Endoluminal Ultrasound Biomicroscopic Imaging and Volumetry of Mouse Colon Tumors.

Ultrasound Med Biol 2021 Jul 22. Epub 2021 Jul 22.

Biomedical Engineering Program, COPPE, Federal University of Rio de Janeiro, Rio de Janeiro, RJ, Brazil; Post-Graduation Program in Surgical Sciences, Department of Surgery, School of Medicine, Federal University of Rio de Janeiro, Rio de Janeiro, RJ, Brazil.

Currently, colonoscopy is considered the gold standard procedure for diagnosis of colorectal cancer (CRC), the third most common cancer in the United States. However, this technique fails to detect flat adenomas, serrated polyps and advanced adenomas, with miss rates of 34%, 27% and 14%, respectively. These miss rates, more frequent than previously supposed, suggest the need for new CRC screening tools. Read More

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Pathogenomics of Streptococcus ilei sp. nov., a newly identified pathogen ubiquitous in human microbiome.

J Microbiol 2021 Aug 24;59(8):792-806. Epub 2021 Jul 24.

Department of Biology, Department of Biomedical and Pharmaceutical Sciences, and Department of Life and Nanopharmaceutical Sciences, Kyung Hee University, Seoul, 02447, Republic of Korea.

Viridans group streptococci are a serious health concern because most of these bacteria cause life-threatening infections, especially in immunocompromised and hospitalized individuals. We focused on two alpha-hemolytic Streptococcus strains (I-G2 and I-P16) newly isolated from an ileostomy effluent of a colorectal cancer patient. We examined their pathogenic potential by investigating their prevalence in human and assessing their pathogenicity in a mouse model. Read More

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Evaluation of Antiproliferative Palladium(II) Complexes of Synthetic Bisdemethoxycurcumin towards In Vitro Cytotoxicity and Molecular Docking on DNA Sequence.

Molecules 2021 Jul 20;26(14). Epub 2021 Jul 20.

Department of Pharmacology, Faculty of Medicine, Pavol Jozef Šafárik University, Trieda SNP 1, 04011 Košice, Slovakia.

Metallodrugs form a large family of therapeutic agents against cancer, among which is cisplatin, a paradigmatic member. Therapeutic resistance and undesired side effects to Pt(II) related drugs, prompts research on different metal-ligand combinations with potentially enhanced biological activity. We present the synthesis and biological tests of novel palladium(II) complexes containing bisdemethoxycurcumin (BDMC) and . Read More

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STAT1 Is Required for Decreasing Accumulation of Granulocytic Cells via IL-17 during Initial Steps of Colitis-Associated Cancer.

Int J Mol Sci 2021 Jul 19;22(14). Epub 2021 Jul 19.

Unidad de Biomedicina, Facultad de Estudios Superiores-Iztacala, Universidad Nacional Autónoma de México, Tlalnepantla, Edo. De México 54090, Mexico.

Signal transducer and activator of transcription 1 (STAT1) acts as a tumor suppressor molecule in colitis-associated colorectal cancer (CAC), particularly during the very early stages, modulating immune responses and controlling mechanisms such as apoptosis and cell proliferation. Previously, using an experimental model of CAC, we reported increased intestinal cell proliferation and faster tumor development, which were consistent with more signs of disease and damage, and reduced survival in STAT1 mice, compared with WT counterparts. However, the mechanisms through which STAT1 might prevent colorectal cancer progression preceded by chronic inflammation are still unclear. Read More

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4-Acetyl-Antroquinonol B Improves the Sensitization of Cetuximab on Both Kras Mutant and Wild Type Colorectal Cancer by Modulating the Expression of Ras/Raf/miR-193a-3p Signaling Axis.

Int J Mol Sci 2021 Jul 14;22(14). Epub 2021 Jul 14.

Division of Gastroenterology and Hepatology, Department of Internal Medicine, School of Medicine, College of Medicine, Taipei Medical University, Taipei 110, Taiwan.

The KRAS mutation is one of the leading driver mutations in colorectal cancer (CRC), and it is usually associated with poor prognosis and drug resistance. Therapies targeting the epidermal growth factor receptor (EFGR) are widely used for end-stage CRC. However, patients with KRAS mutant genes cannot benefit from this therapy because of Ras signaling activation by KRAS mutant genes. Read More

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A Comparative Analysis of the In Vitro Anticancer Activity of Iridium(III) {η-CMeR} Complexes with Variable R Groups.

Int J Mol Sci 2021 Jul 10;22(14). Epub 2021 Jul 10.

Department of Chemistry and Industrial Chemistry, University of Pisa, Via Moruzzi 13, I-56124 Pisa, Italy.

Piano-stool iridium complexes based on the pentamethylcyclopentadienyl ligand (Cp*) have been intensively investigated as anticancer drug candidates and hold much promise in this setting. A systematic study aimed at outlining the effect of Cp* mono-derivatization on the antiproliferative activity is presented here. Thus, the dinuclear complexes [Ir(η-CMeR)Cl(μ-Cl)] (R = Me, ; R = H, ; R = Pr, ; R = 4-CHF, ; R = 4-CHOH, ), their 2-phenylpyridyl mononuclear derivatives [Ir(η-CMeR)(k,kPhPy)Cl] (), and the dimethylsulfoxide complex [Ir{η-CMe(4-CHOH)}Cl(κ-MeS=O)] () were synthesized, structurally characterized, and assessed for their cytotoxicity towards a panel of six human and rodent cancer cell lines (mouse melanoma, B16; rat glioma, C6; breast adenocarcinoma, MCF-7; colorectal carcinoma, SW620 and HCT116; ovarian carcinoma, A2780) and one primary, human fetal lung fibroblast cell line (MRC5). Read More

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Metformin and Niclosamide Synergistically Suppress Wnt and YAP in APC-Mutated Colorectal Cancer.

Cancers (Basel) 2021 Jul 9;13(14). Epub 2021 Jul 9.

Department of Internal Medicine, Institute of Gastroenterology, Yonsei University College of Medicine, Seoul 03722, Korea.

The Wnt and Hippo pathways are tightly coordinated and understanding their reciprocal regulation may provide a novel therapeutic strategy for cancer. Anti-helminthic niclosamide is an effective inhibitor of Wnt and is now in a phase II trial for advanced colorectal cancer (CRC) patients. We found that Axin2, an authentic target gene of canonical Wnt, acts as aYAP phosphorylation activator in APC-mutated CRC. Read More

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NEK7 Promotes Pancreatic Cancer Progression And Its Expression Is Correlated With Poor Prognosis.

Front Oncol 2021 6;11:705797. Epub 2021 Jul 6.

Department of Hepatobiliary Surgery, Peking University Shenzhen Hospital, Shenzhen, China.

The prognosis for pancreatic ductal adenocarcinoma (PDAC) patients is still dismal. Elucidation of associated genomic alteration may provide effective therapeutic strategies for PDAC treatment. NIMA-related protein kinase 7 is widely expressed in various tumors, including breast cancer, colorectal cancer and lung cancer, and promotes the proliferation of liver cancer cells and . Read More

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Genetic mapping of novel modifiers for Apc induced intestinal polyps' development using the genetic architecture power of the collaborative cross mice.

BMC Genomics 2021 Jul 22;22(1):566. Epub 2021 Jul 22.

Department of Clinical Microbiology & Immunology, Sackler Faculty of Medicine, Ramat Aviv, 69978, Tel-Aviv, Israel.

Background: Familial adenomatous polyposis is an inherited genetic disease, characterized by colorectal polyps. It is caused by inactivating mutations in the Adenomatous polyposis coli (Apc) gene. Mice carrying a nonsense mutation in the Apc gene at R850, which is designated Apc (Multiple intestinal neoplasia), develop intestinal adenomas. Read More

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BTF3-mediated regulation of BMI1 promotes colorectal cancer through influencing epithelial-mesenchymal transition and stem cell-like traits.

Int J Biol Macromol 2021 Jul 19. Epub 2021 Jul 19.

Department of Colorectal and Anal Surgery, The First Hospital of Jilin University, Changchun 130000, PR China. Electronic address:

The critical roles of transcription factors in cell differentiation and the delineation of cell phenotypes have been reported. The current study aimed to characterize the functions of the basic transcription factor 3 (BTF3) gene and its regulation of the intestinal stem cell marker B cell-specific Moloney murine leukemia virus insertion site 1 (BMI1) gene in colorectal cancer (CRC). Stem cell-like traits and epithelial-mesenchymal transition (EMT) of cultured human CRC cell line HCT116 were evaluated by CD133+ subpopulation counting, colony formation, tumorosphere generation, and expression of EMT-specific markers and stem cell markers. Read More

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Type 2 immunity is maintained during cancer-associated adipose tissue wasting.

Immunother Adv 2021 Jan 2;1(1):ltab011. Epub 2021 Jun 2.

Department of Cancer Immunology and Virology, Dana Farber Cancer Institute, Boston, MA, USA.

Objectives: Cachexia is a systemic metabolic disorder characterized by loss of fat and muscle mass, which disproportionately impacts patients with gastrointestinal malignancies such as pancreatic cancer. While the immunologic shifts contributing to the development of other adipose tissue (AT) pathologies such as obesity have been well described, the immune microenvironment has not been studied in the context of cachexia.

Methods: We performed bulk RNA-sequencing, cytokine arrays, and flow cytometry to characterize the immune landscape of visceral AT (VAT) in the setting of pancreatic and colorectal cancers. Read More

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January 2021

Juniperus communis extract induces cell cycle arrest and apoptosis of colorectal adenocarcinoma in vitro and in vivo.

Braz J Med Biol Res 2021 16;54(10):e10891. Epub 2021 Jul 16.

Department of Medical Laboratory and Biotechnology, Chung Shan Medical University, Taichung, Republic of China, Taiwan.

Juniperus communis (JCo) is a well-known traditional Chinese medicinal plant that has been used to treat wounds, fever, swelling, and rheumatism. However, the mechanism underlying the anticancer effect of JCo extract on colorectal cancer (CRC) has not yet been elucidated. This study investigated the anticancer effects of JCo extract in vitro and in vivo as well as the precise molecular mechanisms. Read More

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Toward Elucidating the Physiological Impacts of Residual Stresses in the Colorectum.

J Biomech Eng 2021 Jul 21. Epub 2021 Jul 21.

Department of Mechanical Engineering, University of Connecticut, Storrs, CT, USA; Department of Biomedical Engineering, University of Connecticut, Storrs, CT, USA.

Irritable bowel syndrome afflicts 10-20% of the global population, causing visceral pain with increased sensitivity to colorectal distension and normal bowel movements. Understanding and predicting these biomechanics will further advance our understanding of visceral pain and complement the existing literature on visceral neurophysiology. We recently performed a series of experiments at three longitudinal segments (colonic, intermediate, and rectal) of the distal 30 mm of colorectums of mice. Read More

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Tumor RNA-loaded nanoliposomes increases the anti-tumor immune response in colorectal cancer.

Drug Deliv 2021 Dec;28(1):1548-1561

Department of Gastrointestinal Surgery, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Zhejiang, China.

Purpose: Tumor RNA vaccines can activate dendritic cells to generate systemic anti-tumor immune response. However, due to easily degraded of RNA, direct RNA vaccine is less effective. In this study, we optimized the method for preparing PEGylated liposom-polycationic DNA complex (LPD) nanoliposomes, increased encapsulate amount of total RNA derived from CT-26 colorectal cancer cells. Read More

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December 2021

Development, Characterization, and Evaluation of SLN-Loaded Thermoresponsive Hydrogel System of Topotecan as Biological Macromolecule for Colorectal Delivery.

Biomed Res Int 2021 3;2021:9968602. Epub 2021 Jul 3.

Nanomedicine Research Group, Department of Pharmacy, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, Pakistan.

Background: Chemotherapeutic drugs cause severe toxicities if administered unprotected, without proper targeting, and controlled release. In this study, we developed topotecan- (TPT-) loaded solid lipid nanoparticles (SLNs) for their chemotherapeutic effect against colorectal cancer. The TPT-SLNs were further incorporated into a thermoresponsive hydrogel system (TRHS) (TPT-SLNs-TRHS) to ensure control release and reduce toxicity of the drug. Read More

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PLA2G4A promotes right-sided colorectal cancer progression by inducing CD39+γδ Tregs polarization.

JCI Insight 2021 Jul 20. Epub 2021 Jul 20.

Department of Colorectal Cancer, Tianjin Medical University Cancer Institute and Hospital, Tianjin, China.

γδ T cell is a promising candidate cell in tumor immunotherapy. However, γδ T cells polarized to CD39+γδ Tregs upon colorectal cancer (CRC) induction and the underlying mechanism remains unclear. Here, we discovered that the frequency of CD39+γδ Tregs, which positively correlated with poor prognosis, was significantly higher in right-sided CRC (RSCRC) than in the left-sided CRC (LSCRC). Read More

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Growth Suppression of Cancer Spheroids With Mutated KRAS by Low-toxicity Compounds from Natural Products.

Anticancer Res 2021 Aug;41(8):4061-4070

Department of Cell Biology, Faculty of Medicine, Fukuoka University, Fukuoka, Japan

Background/aim: Among compounds from natural products selectively suppressing the growth of cancer spheroids, which have mutant (mt) KRAS, NP910 was selected and its derivatives explored.

Materials And Methods: The area of HKe3 spheroids expressing wild type (wt) KRAS (HKe3-wtKRAS) and mtKRAS (HKe3-mtKRAS) were measured in three-dimensional floating (3DF) cultures treated with 18 NP910 derivatives. The 50% cell growth inhibition (GI50) was determined by long-term 3DF (LT3DF) culture and nude mice assay. Read More

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Claudin-7 deficiency promotes stemness properties in colorectal cancer through Sox9-mediated Wnt/β-catenin signalling.

J Transl Med 2021 Jul 19;19(1):311. Epub 2021 Jul 19.

Department of Oncology, Beijing Shijitan Hospital, Capital Medical University, Beijing, 100038, China.

Background: Colorectal cancer (CRC) is a common malignant tumour of the digestive tract that is characterized by high patient morbidity and mortality rates. Claudin-7 (Cldn7), a tight junction protein, was recently reported to function as a candidate tumour suppressor gene in CRC. Our previous study demonstrated that the large intestine of C57/BL6 mice showed intestinal adenomas and abnormal Ki67 expression and distribution in the intestinal crypt when Cldn7 was knocked out. Read More

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Dual-modified PCL-PEG nanoparticles for improved targeting and therapeutic efficacy of docetaxel against colorectal cancer.

Pharm Dev Technol 2021 Jul 19:1-33. Epub 2021 Jul 19.

Department of Pharmacy, Daping Hospital, Army Medical University, Chongqing, 400042, China.

Polycaprolactone-poly (ethylene glycol) block copolymer (PCL-PEG) based nanoparticles were prepared for the intravenous administration of docetaxel (DTX). PCL-PEG-Tyr and PCL-PEG-Ang were synthesized by using tyrosine (Tyr) and angiopep-2 (Ang) as coupling ligands, and dual-modified PCL-PEG based nanoparticles (PCL-PEG-Tyr/Ang) were prepared. The physicochemical properties, in vitro drug release, in vitro cytotoxicity, in vitro cellular uptake efficiency, in vivo biodistribution and in vivo antitumor efficacy of PCL-PEG based nanoparticles were investigated. Read More

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Protective Effect of Saffron in Mouse Colitis Models Through Immune Modulation.

Dig Dis Sci 2021 Jul 17. Epub 2021 Jul 17.

Department of Medicine and Cancer Center, Howard University College of Medicine, Washington, DC, USA.

Background: People with inflammatory bowel disease (IBD) including ulcerative colitis are at risk for colorectal cancer. Despite available effective drugs used to treat IBD, many patients fail or lose response over time with some displaying drug-induced adverse events. Saffron (Crocus sativus) has been reported to have anti-inflammatory properties. Read More

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