364,555 results match your criteria multiple cellular


Trait positive affect buffers the association between experimental sleep disruption and inflammation.

Psychoneuroendocrinology 2021 Apr 28;129:105240. Epub 2021 Apr 28.

Department of Psychiatry and Behavioral Sciences, Johns Hopkins University School of Medicine, Baltimore, MD, USA.

Background: Sleep disturbances and insufficient sleep are highly prevalent. Both clinical sleep disorders and multiple forms of experimental sleep loss predict heightened inflammation. As such, it is necessary to investigate potential protective factors. Read More

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Multi-epitope DnaK peptide Vaccine Accords Protection against lethal S. typhimurium Challenge: Elicits both Cell mediated immunity and long-lasting Serum-Neutralizing Antibody Titers.

Pharmacol Res 2021 May 8:105652. Epub 2021 May 8.

Defence Institute of Physiology and Allied Sciences, Lucknow Road, Timarpur, Delhi -110054. Electronic address:

Typhoid vaccine development has been impeded by inability of currently available vaccines to induce cellular immunity along with neutralizing antibodies against all serovars of S. Typhi and S. Paratyphi. Read More

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SARS-COV-2 antibody dynamics and B-cell memory response over-time in COVID-19 convalescent subjects.

Clin Microbiol Infect 2021 May 8. Epub 2021 May 8.

Laura Schwarz-Kipp Research of Autoimmune Diseases, Sackler School of Medicine Tel-Aviv University, Neuroimmunology Laboratory at the Multiple Sclerosis Center, and Blood Bank, Sheba Medical Center, Ramat-Gann, Israel.

Objectives: Worldwide spread of COVID-19 disease highlights the need for assessment of long-term humoral immunity in convalescent subjects. Our objectives were to evaluate long-term IgG SARS-CoV-2 antibody response and B-cell memory response in COVID-19 convalescent subjects.

Methods: Blood samples were collected from a cohort of subjects recovering from COVID-19 disease and from healthy subjects that donated blood. Read More

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The multifaceted roles of macrophages in bone regeneration: A story of polarization, activation and time.

Acta Biomater 2021 May 8. Epub 2021 May 8.

Julius Wolff Institut, Berlin Institute of Health at Charité - Universitätsmedizin Berlin, Augustenburger Platz 1, 13353 Berlin, Germany; Berlin Institute of Health at Charité - Universitätsmedizin Berlin, BIH Center for Regenerative Therapies (BCRT), Charitéplatz 1, 10117 Berlin, Germany. Electronic address:

To present knowledge, macrophages are found in all tissues of the human body. They are a cell population with high plasticity which come with a multitude of functions which appear to be adapted to the respective tissue niche and micro-environment in which they reside. Bone harbors multiple macrophage subpopulations, with the osteoclasts as classical representative of a bone resorbing cells and osteomacs as a bone tissue resident macrophage first described by the expression of F4/80. Read More

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TSC1 binding to lysosomal PIPs is required for TSC complex translocation and mTORC1 regulation.

Mol Cell 2021 Apr 30. Epub 2021 Apr 30.

University of Münster, Institute of Biochemistry, 48149 Münster, Germany; University of Osnabrück, Department of Biology/Chemistry, Structural Biology Section, 49076 Osnabrück, Germany. Electronic address:

The TSC complex is a critical negative regulator of the small GTPase Rheb and mTORC1 in cellular stress signaling. The TSC2 subunit contains a catalytic GTPase activating protein domain and interacts with multiple regulators, while the precise function of TSC1 is unknown. Here we provide a structural characterization of TSC1 and define three domains: a C-terminal coiled-coil that interacts with TSC2, a central helical domain that mediates TSC1 oligomerization, and an N-terminal HEAT repeat domain that interacts with membrane phosphatidylinositol phosphates (PIPs). Read More

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Establishment of drug-resistant cell lines under the treatment with chemicals acting through different mechanisms.

Chem Biol Interact 2021 May 8:109510. Epub 2021 May 8.

St. Petersburg State Institute of Technology (Technical University), Moskovskii Prospect, 26, St. Petersburg, 190013, Russia.

The problem of chemoresistance development is an inescapable flipside of modern oncotherapy, in particular for сolorectal cancer patients. The search for or development of drugs effective against resistant tumors involves the use of model resistant cell lines in vitro. To obtain such lines, we reproduced the development of chemoresistance of human colon adenocarcinoma cells under the treatment with drugs of different mechanisms, a cytostatic (paclitaxel) and a targeted agent (Nutlin-3a, an inhibitor of p53-Mdm2 protein-protein interaction). Read More

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Effects of cathode/anode electron accumulation on soil microbial fuel cell power generation and heavy metal removal.

Environ Res 2021 May 8:111217. Epub 2021 May 8.

School of Energy and Environment, Southeast University, Nanjing 210096, China. Electronic address:

Microbial fuel cells (MFCs) with different electrode configurations were constructed to study the mechanism of influence of multiple current paths on their electrical performance and the removal of heavy metals in soil. Three types of MFCs were constructed, namely, double anode-single cathode (DASC), single anode-dual cathode (SADC), and single anode-single cathode (SASC). The total electricity generation of the three kinds of MFC was similar: 143. Read More

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Infection of humanized mice with a novel phlebovirus presented pathogenic features of sever fever with thrombocytopenia syndrome.

PLoS Pathog 2021 May 11;17(5):e1009587. Epub 2021 May 11.

School of Life Sciences, Ningxia University, Yinchuan, P.R. China.

Severe fever with thrombocytopenia syndrome virus (SFTSV) is a tick-borne emerging phlebovirus with high mortality rates of 6.0 to 30%. SFTSV infection is characterized by high fever, thrombocytopenia, leukopenia, hemorrhage and multiple organ failures. Read More

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Metachronous carcinogenesis of superficial esophagus squamous cell carcinoma after endoscopic submucosal dissection: incidence and risk stratification during long-term observation.

Esophagus 2021 May 11. Epub 2021 May 11.

Department of Gastroenterology, Toranomon Hospital, 2-2-2 Toranomon, Minato-ku, Tokyo, 105-0001, Japan.

Background: This study aimed to reveal long-term outcomes, such as incidence of metachronous esophageal and head and neck squamous cell carcinomas and overall survival rate, through long-term observation of patients with esophageal carcinoma post-endoscopic submucosal dissection.

Methods: Risk of metachronous carcinogenesis was evaluated in 88 patients with intramucosal esophageal carcinoma (without history of metachronous esophageal or head and neck squamous cell carcinomas) who underwent endoscopic submucosal dissection from 2007 to 2008 and were endoscopically observed for > 3 years. Histologically, the papillary vessel is defined as the clock gear-like structure composed of capillaries directly penetrating the epithelium (starting from the lamina propria) and covering at least two-thirds of it, around which the tumor cells are arranged in a spiral pattern. Read More

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Role of Histone Deacetylases in Monocyte Function in Health and Chronic Inflammatory Diseases.

Rev Physiol Biochem Pharmacol 2021 May 12. Epub 2021 May 12.

Department of Pharmacology and Toxicology, Universidad de Navarra, School of Pharmacy and Nutrition, Pamplona, Spain.

Histone deacetylases (HDACs) are a family of 18 members that participate in the epigenetic regulation of gene expression. In addition to histones, some HDACs also deacetylate transcription factors and specific cytoplasmic proteins.Monocytes, as part of the innate immune system, maintain tissue homeostasis and help fight infections and cancer. Read More

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MAT2: Manifold alignment of single-cell transcriptomes with cell triplets.

Bioinformatics 2021 May 10. Epub 2021 May 10.

Institute of Science and Technology for Brain-Inspired Intelligence, Fudan University, Shanghai 200433, China.

Motivation: Aligning single-cell transcriptomes is important for the joint analysis of multiple single-cell RNA sequencing datasets, which in turn is vital to establishing a holistic cellular landscape of certain biological processes. Although numbers of approaches have been proposed for this problem, most of which only consider mutual neighbors when aligning the cells without taking into account known cell type annotations.

Results: In this work, we present MAT2 that aligns cells in the manifold space with a deep neural network employing contrastive learning strategy. Read More

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MAT2: Manifold alignment of single-cell transcriptomes with cell triplets.

Bioinformatics 2021 May 11. Epub 2021 May 11.

Institute of Science and Technology for Brain-Inspired Intelligence, Fudan University, Shanghai 200433, China.

Motivation: Aligning single-cell transcriptomes is important for the joint analysis of multiple single-cell RNA sequencing datasets, which in turn is vital to establishing a holistic cellular landscape of certain biological processes. Although numbers of approaches have been proposed for this problem, most of which only consider mutual neighbors when aligning the cells without taking into account known cell type annotations.

Results: In this work, we present MAT2 that aligns cells in the manifold space with a deep neural network employing contrastive learning strategy. Read More

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GlcNAc is a mast-cell chromatin-remodeling oncometabolite that promotes systemic mastocytosis aggressiveness.

Blood 2021 May 11. Epub 2021 May 11.

Centre de Recherche en Cancérologie de Marseille - Inserm U1068, CNRS UMR7258, Aix-Marseille Université, Institut Paoli-Calmettes, Marseille, France.

Systemic mastocytosis (SM) is a KIT-driven hematopoietic neoplasm characterized by the excessive accumulation of neoplastic mast cells (MCs) in various organs and, mainly, the bone marrow (BM). Multiple genetic and epigenetic mechanisms contribute to the onset and severity of SM. However, little is known to date about the metabolic underpinnings underlying SM aggressiveness, which has thus far impeded the development of strategies to leverage metabolic dependencies when existing KIT-targeted treatments fail. Read More

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The role of sigma 1 receptor in organization of endoplasmic reticulum signaling microdomains.

Elife 2021 May 11;10. Epub 2021 May 11.

Department of Physiology, UT Southwestern Medical Center at Dallas, Dallas, United States.

Sigma 1 receptor (S1R) is a 223-amino-acid-long transmembrane endoplasmic reticulum (ER) protein. S1R modulates activity of multiple effector proteins and is a well-established drug target. However, signaling functions of S1R in cells are poorly understood. Read More

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Limited inhibition of multiple nodes in a driver network blocks metastasis.

Elife 2021 May 11;10. Epub 2021 May 11.

Ben May Department for Cancer Research, University of Chicago, Chicago, United States.

Metastasis suppression by high-dose, multi-drug targeting is unsuccessful due to network heterogeneity and compensatory network activation. Here we show that targeting driver network signaling capacity by limited inhibition of core pathways is a more effective anti-metastatic strategy. This principle underlies the action of a physiological metastasis suppressor, Raf Kinase Inhibitory Protein (RKIP), that moderately decreases stress-regulated MAP kinase network activity, reducing output to transcription factors such as pro-metastastic BACH1 and motility-related target genes. Read More

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[Development of a luminescence real-time method for monitoring live bacteria during phage lysis].

Sheng Wu Gong Cheng Xue Bao 2021 Apr;37(4):1406-1414

State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.

The toxin-producing bacterium Vibrio cholerae can cause severe diarrhea and has caused seven global pandemics. Traditional viable cell counts and phage plaques are commonly used to evaluate the efficacy of virulent phage clearance of V. cholerae, but these operations are time-consuming and labor-intensive, and difficult to provide real-time changes. Read More

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Cornuside alleviates experimental autoimmune encephalomyelitis by inhibiting Th17 cell infiltration into the central nervous system.

J Zhejiang Univ Sci B 2021 May;22(5):421-430

College of Pharmacy, Zhejiang Chinese Medical University, Hangzhou 310053, China.

The present study was conducted to clarify the therapeutic effect of cornuside on experimental autoimmune encephalomyelitis (EAE) and its influence on T helper 17 (Th17) cell and regulatory T (Treg) cell infiltration into the central nervous system. Rats were randomly placed into four treatment groups: control, EAE, EAE+cornuside, and EAE+prednisolone. The neurological function scores of rats were assessed daily. Read More

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hu.MAP 2.0: integration of over 15,000 proteomic experiments builds a global compendium of human multiprotein assemblies.

Mol Syst Biol 2021 May;17(5):e10016

Department of Molecular Biosciences, Center for Systems and Synthetic Biology, University of Texas, Austin, TX, USA.

A general principle of biology is the self-assembly of proteins into functional complexes. Characterizing their composition is, therefore, required for our understanding of cellular functions. Unfortunately, we lack knowledge of the comprehensive set of identities of protein complexes in human cells. Read More

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Leptomeningeal and intraventricular myelomatosis manifesting an aggressive form of communicating hydrocephalus.

Neuropathology 2021 May 10. Epub 2021 May 10.

Department of Neuropathology, Institute of Brain Science, Hirosaki University Graduate School of Medicine, Hirosaki, Japan.

Leptomeningeal myelomatosis (LMM) is a fatal complication that occurs in < 1% of patients with multiple myeloma. Many patients with LMM present with neurologic symptoms referable to cranial neuropathies, while the manifestation of communicating hydrocephalus has been underrecognized. A Japanese man with Bence Jones protein-κ multiple myeloma developed fever and headache at age 54 years. Read More

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miR-4286 functions in osteogenesis and angiogenesis via targeting histone deacetylase 3 and alleviates alcohol-induced bone loss in mice.

Cell Prolif 2021 May 10:e13054. Epub 2021 May 10.

Department of Orthopedic Surgery, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, China.

Objectives: Alcohol consumption is one of the leading factors contributing to premature osteopenia. MicroRNA (miRNA) coordinates a cascade of anabolic and catabolic processes in bone homeostasis and dynamic vascularization. The aim was to investigate the protective role of miR-4286 in alcohol-induced bone loss and its mechanism. Read More

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Autoimmune Processes Involved in Organ System Failure Following Infection with SARS-CoV-2.

Adv Exp Med Biol 2021 ;1318:355-368

Biological Safety and Security, Frederick, MD, USA.

During the COVID-19 pandemic associated with high incidence, transmissibility, and mortality, this chapter focuses on three phases of the disease: initial exposure, initiation of the immune response to the agent, and finally, an inflammatory/autoimmune-like presentation with pulmonary, neurological, and renal failure and disseminated intravascular coagulation which occurs in a small proportion of the patients. The elegant demonstration of the site of interaction between the spike (S) protein of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which is the causative agent of COVID-19, and the ACE (angiotensin-converting enzyme) 2 receptor of cells distributed throughout the body has enabled research efforts to develop pharmacological and immune countermeasures to the viral phase of the disease. This chapter rapidly reviews the molecular and structural organization of SARS-CoV-2 and its interaction with ACE2. Read More

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Neurologic Manifestations of COVID-19.

Adv Exp Med Biol 2021 ;1318:343-353

Research Center for Immunodeficiencies, Children's Medical Center, Tehran University of Medical Sciences, Tehran, Iran.

Neurological manifestations of novel coronavirus disease (COVID-19) are reported to occur in as much as 37% of the affected patients. These manifestations range from headache and dizziness to altered mental status and consciousness, anosmia, ageusia, sensory disturbances, and stroke. The mechanisms by which the neurological symptoms arise are not yet determined but may either proceed as an indirect consequence of systemic hyperinflammation or result from the direct invasion of the virus to neural and glial cells. Read More

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Modeling the evolutionary architectures of transcribed human enhancer sequences reveals distinct origins, functions, and associations with human-trait variation.

Mol Biol Evol 2021 May 10. Epub 2021 May 10.

Vanderbilt Genetics Institute, Vanderbilt University, Nashville, TN, USA.

Motivation: Despite the importance of gene regulatory enhancers in human biology and evolution, we lack a comprehensive model of enhancer evolution and function. This substantially limits our understanding of the genetic basis of species divergence and our ability to interpret the effects of non-coding variants on human traits.

Results: To explore enhancer sequence evolution and its relationship to regulatory function, we traced the evolutionary origins of transcribed human enhancer sequences with activity across diverse tissues and cellular contexts from the FANTOM5 consortium. Read More

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Mechanobiology of Pulmonary Diseases: A Review of Engineering Tools to Understand Lung Mechanotransduction.

J Biomech Eng 2021 May 11. Epub 2021 May 11.

Division of Pulmonary, Critical Care, and Sleep Medicine, Department of Internal Medicine, The Davis Heart and Lung Research Institute, The Ohio State University Wexner Medical Center, 473 West 12th Avenue Columbus, OH 43210; Department of Biomedical Engineering, The Ohio State University, 140 West 19th Avenue Columbus, OH 43210.

Cells within the lung microenvironment are continuously subjected to dynamic mechanical stimuli which is converted into biochemical signaling events in a process known as mechanotransduction. In pulmonary diseases, the abrogated mechanical conditions modify the homeostatic signaling which influences cellular phenotype and disease progression. The use of in vitro models has significantly expanded our understanding of lung mechanotransduction mechanisms. Read More

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ORAI1 establishes resistance to SARS-CoV-2 infection by regulating tonic type I interferon signaling.

bioRxiv 2021 May 4. Epub 2021 May 4.

ORAI1 and STIM1 are the critical mediators of store-operated Ca entry by acting as the pore subunit and an endoplasmic reticulum-resident signaling molecule, respectively. In addition to Ca signaling, STIM1 is also involved in regulation of a cytosolic nucleic acid sensing pathway. Using and knockout cells, we examined their contribution to the host response to SARS-CoV-2 infection. Read More

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Extraction of nuclei from archived postmortem tissues for single-nucleus sequencing applications.

Nat Protoc 2021 May 10. Epub 2021 May 10.

McGill Group for Suicide Studies, Douglas Mental Health University Institute, Montreal, Canada.

Single-cell and single-nucleus sequencing techniques are a burgeoning field with various biological, biomedical and clinical applications. Numerous high- and low-throughput methods have been developed for sequencing the RNA and DNA content of single cells. However, for all these methods, the key requirement is high-quality input of a single-cell or single-nucleus suspension. Read More

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Integrating barcoded neuroanatomy with spatial transcriptional profiling enables identification of gene correlates of projections.

Nat Neurosci 2021 May 10. Epub 2021 May 10.

Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, USA.

Functional circuits consist of neurons with diverse axonal projections and gene expression. Understanding the molecular signature of projections requires high-throughput interrogation of both gene expression and projections to multiple targets in the same cells at cellular resolution, which is difficult to achieve using current technology. Here, we introduce BARseq2, a technique that simultaneously maps projections and detects multiplexed gene expression by in situ sequencing. Read More

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Wireless multilateral devices for optogenetic studies of individual and social behaviors.

Nat Neurosci 2021 May 10. Epub 2021 May 10.

State Key Laboratory of Structural Analysis for Industrial Equipment, Department of Engineering Mechanics, Dalian University of Technology, Dalian, P.R. China.

Advanced technologies for controlled delivery of light to targeted locations in biological tissues are essential to neuroscience research that applies optogenetics in animal models. Fully implantable, miniaturized devices with wireless control and power-harvesting strategies offer an appealing set of attributes in this context, particularly for studies that are incompatible with conventional fiber-optic approaches or battery-powered head stages. Limited programmable control and narrow options in illumination profiles constrain the use of existing devices. Read More

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RRx-001 ameliorates inflammatory diseases by acting as a potent covalent NLRP3 inhibitor.

Cell Mol Immunol 2021 May 10. Epub 2021 May 10.

Hefei National Laboratory for Physical Sciences at Microscale, the CAS Key Laboratory of Innate Immunity and Chronic Disease, School of Basic Medical Sciences, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, China.

The NLRP3 inflammasome plays a crucial role in innate immune-mediated inflammation and contributes to the pathogenesis of multiple autoinflammatory, metabolic and neurodegenerative diseases, but medications targeting the NLRP3 inflammasome are not available for clinical use. RRx-001 is a well-tolerated anticancer agent currently being investigated in phase III clinical trials, but its effects on inflammatory diseases are not known. Here, we show that RRx-001 is a highly selective and potent NLRP3 inhibitor that has strong beneficial effects on NLRP3-driven inflammatory diseases. Read More

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The genome of Nautilus pompilius illuminates eye evolution and biomineralization.

Nat Ecol Evol 2021 May 10. Epub 2021 May 10.

Key Laboratory of Tropical Marine Bio-resources and Ecology and Guangdong Provincial Key Laboratory of Applied Marine Biology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, China.

Nautilus is the sole surviving externally shelled cephalopod from the Palaeozoic. It is unique within cephalopod genealogy and critical to understanding the evolutionary novelties of cephalopods. Here, we present a complete Nautilus pompilius genome as a fundamental genomic reference on cephalopod innovations, such as the pinhole eye and biomineralization. Read More

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