100,277 results match your criteria multidrug resistance


Relation between ABCB1 overexpression and COX2 and ALOX5 genes in human erythroleukemia cell lines.

Prostaglandins Other Lipid Mediat 2021 May 8:106553. Epub 2021 May 8.

Laboratório de Cultura Celular, ICB, FURG, RS, Brazil; Programa de Pós-Graduação em Ciências Fisiológicas, ICB, FURG, RS, Brazil. Electronic address:

This study aimed to characterize the relationship between the COX2 and ALOX5 genes, as well as their link with the multidrug resistance (MDR) phenotype in sensitive (K562) and MDR (K562-Lucena and FEPS) erythroleukemia cells. For this, the inhibitors of 5-LOX (zileuton) and COX-2 (acetylsalicylic acid-ASA) and cells with the silenced ABCB1 gene were used. The treatment with ASA caused an increase in the gene expression of COX2 and ABCB1 in both MDR cell lines, and a decrease in the expression of ALOX5 in the FEPS cells. Read More

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Establishment of drug-resistant cell lines under the treatment with chemicals acting through different mechanisms.

Chem Biol Interact 2021 May 8:109510. Epub 2021 May 8.

St. Petersburg State Institute of Technology (Technical University), Moskovskii Prospect, 26, St. Petersburg, 190013, Russia.

The problem of chemoresistance development is an inescapable flipside of modern oncotherapy, in particular for сolorectal cancer patients. The search for or development of drugs effective against resistant tumors involves the use of model resistant cell lines in vitro. To obtain such lines, we reproduced the development of chemoresistance of human colon adenocarcinoma cells under the treatment with drugs of different mechanisms, a cytostatic (paclitaxel) and a targeted agent (Nutlin-3a, an inhibitor of p53-Mdm2 protein-protein interaction). Read More

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Subcellular distribution of ezrin/radixin/moesin and their roles in the cell surface localization and transport function of P-glycoprotein in human colon adenocarcinoma LS180 cells.

PLoS One 2021 11;16(5):e0250889. Epub 2021 May 11.

Laboratory of Clinical Pharmaceutics, Faculty of Pharmacy, Osaka Ohtani University, Tondabayashi, Osaka, Japan.

The ezrin/radixin/moesin (ERM) family proteins act as linkers between the actin cytoskeleton and P-glycoprotein (P-gp) and regulate the plasma membrane localization and functionality of the latter in various cancer cells. Notably, P-gp overexpression in the plasma membrane of cancer cells is a principal factor responsible for multidrug resistance and drug-induced mutagenesis. However, it remains unknown whether the ERM proteins contribute to the plasma membrane localization and transport function of P-gp in human colorectal cancer cells in which the subcellular localization of ERM has yet to be determined. Read More

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[The impact of genetic and environmental regulation on the expression of antibiotic resistance genes in Enterobacteriaceae].

Sheng Wu Gong Cheng Xue Bao 2021 Apr;37(4):1092-1106

College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.

Antibiotic resistance is a major global concern and challenge in the 21st century. Enterobacteriaceae are one of the important pathogens of hospital-acquired infections. With the increasing use of antibiotics in clinical practice, a variety of drug-resistant Enterobacteriaceae, especially multidrug-resistant Enterobacteriaceae have emerged, posing an increasingly serious threat to human health. Read More

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Bacterial Biofilms on Tracheostomy Tubes.

Indian J Otolaryngol Head Neck Surg 2021 May 6:1-5. Epub 2021 May 6.

Department of Otorhinolaryngology, Yenepoya Medical College, Mangalore, Karnataka India.

Tracheostomy is a commonly performed airway surgery for critically ill patients. Tracheostomy tube is an indwelling prosthesis, providing potential surface for growth of bacteria. Biofilm formation by bacteria as a self-protective mechanism, has led to worrisome antibacterial resistance and thus higher rate of nosocomial infections. Read More

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Impact of the inclusion of an aminoglycoside to the initial empirical antibiotic therapy for Gram-negative bloodstream infections in hematological neutropenic patients: a propensity-matched cohort study (AMINOLACTAM study).

Antimicrob Agents Chemother 2021 May 10. Epub 2021 May 10.

Infectious Disease Department, Bellvitge University Hospital, Hospitalet del Llobregat, Barcelona, Spain.

To test the hypothesis that the addition of an aminoglycoside to a ß-lactam antibiotic could provide better outcomes than ß-lactam monotherapy for the initial empirical treatment of hematological neutropenic patients with subsequently documented Gram-negative bacilli (GNB) bloodstream infection (BSI). Multinational, retrospective, cohort study of GNB BSI episodes in hematological neutropenic patients in six centers (2010-2017). Combination therapy (ß-lactam plus aminoglycoside) was compared to ß-lactam monotherapy. Read More

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Characterization of KPC-82, a KPC-2 variant conferring resistance to ceftazidime-avibactam in a carbapenem non-susceptible, clinical isolate of .

Antimicrob Agents Chemother 2021 May 10. Epub 2021 May 10.

Multidrug-Resistant Organism Repository and Surveillance Network (MRSN), Walter Reed Army Institute of Research, Silver Spring, Maryland, USA

KPC-82 is a KPC-2 variant, identified in a carbapenem non-susceptible that confers high-level resistance to ceftazidime-avibactam. Genomic analysis revealed that is carried by a chromosomally integrated Tn transposon (disrupting porin gene ) and evolved by a 6-nucleotide tandem-repeat duplication causing a two-amino-acid insertion (Ser-Asp) within the Ala-Ser loop. Similar to related KPC variants, KPC-82 showed decreased carbapenemase activity when expressed in a heterologous background and remained susceptible to carbapenem/β-lactamase inhibitor combinations. Read More

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Population genomics study of Vibrio alginolyticus.

Yi Chuan 2021 Apr;43(4):350-361

State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, China.

Vibrio alginolyticus is a Gram-negative bacillus that causes vibriosis to human and aquatic products, including fish, shrimp and shellfish. It poses a threat to public health and causes enormous economic losses to the aquaculture industry. However, research on genetic diversity and pathogenicity-related genetic elements based on whole genome is still lacking. Read More

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Novel chromosomal insertions of ISEcp1-bla and diverse antimicrobial resistance genes in Zambian clinical isolates of Enterobacter cloacae and Escherichia coli.

Antimicrob Resist Infect Control 2021 May 10;10(1):79. Epub 2021 May 10.

Division of Infection and Immunity, Research Center for Zoonosis Control, Hokkaido University, Sapporo, Japan.

Background: The epidemiology of extended-spectrum β-lactamases (ESBLs) has undergone dramatic changes, with CTX-M-type enzymes prevailing over other types. bla genes, encoding CTX-M-type ESBLs, are usually found on plasmids, but chromosomal location is becoming common. Given that bla-harboring strains often exhibit multidrug resistance (MDR), it is important to investigate the association between chromosomally integrated bla and the presence of additional antimicrobial resistance (AMR) genes, and to identify other relevant genetic elements. Read More

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tRNA-derived fragments as novel potential biomarkers for relapsed/refractory multiple myeloma.

BMC Bioinformatics 2021 May 11;22(1):238. Epub 2021 May 11.

Department of Blood Transfusion, The Third Xiangya Hospital of Central South University, Changsha, 410000, China.

Background: tRNA-derived fragments have been reported to be key regulatory factors in human tumors. However, their roles in the progression of multiple myeloma remain unknown.

Results: This study employed RNA-sequencing to explore the expression profiles of tRFs/tiRNAs in new diagnosed MM and relapsed/refractory MM samples. Read More

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Soil type shapes the antibiotic resistome profiles of long-term manured soil.

Sci Total Environ 2021 Apr 27;786:147361. Epub 2021 Apr 27.

College of Resources and Environment, Southwest University, Chongqing 400716, China. Electronic address:

Animal manure fertilization facilitates the proliferation and dissemination of antibiotic resistance genes (ARGs) in soil, posing high risks to humans and ecosystem health. Although studies suggest that soil types could shape the ARG profiles in greenhouse soil, there is still a lack of comparative studies on the fate of ARGs in different types of manured soils under field trials. Thus, a metagenomic approach was used to decipher the fate of ARGs in 12-year long-term fertilized (inorganic fertilizer, compost manure and a mix of them) acidic, near-neutral and alkaline soils. Read More

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Carbapenem Resistance in Acinetobacter baumannii, and their Importance in Hospital-acquired Infections: A Scientific Review.

J Appl Microbiol 2021 May 10. Epub 2021 May 10.

Center for Surgical Infections, Drexel University School of Biomedical Engineering, Science & Health Systems, USA.

Carbapenem is an important therapy for serious hospital-acquired infections and for the care of patients affected by multidrug-resistant organisms, specifically Acinetobacter baumannii; however, with the global increase of carbapenem-resistant Ac. baumannii, this pathogen has significantly threatened public health. Thus, there is a pressing need to better understand this pathogen in order to develop novel treatments and control strategies for dealing with Ac. Read More

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Genetic diversity and evolution of the virulence plasmids encoding aerobactin and salmochelin in .

Virulence 2021 Dec;12(1):1323-1333

Department of Laboratory Medicine, Huashan Hospital, Shanghai Medical College, Fudan University, Shanghai, China.

Virulence plasmids of hypervirulent (hvKp) have the potential to transfer to drug-resistant strains or integrate with other plasmids, facilitating the genome evolution of threatening pathogens. We conducted an in-depth analysis of the publicly available 156 complete genome sequences of hvKp together with a multi-region clinical cohort of 171 hvKp strains from China to provide evidence for the virulence plasmid evolution. Virulence plasmids were frequently detected in the ST23 and ST11 strains. Read More

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December 2021

Diagnostic stewardship based on patient profiles: differential approaches in acute versus chronic infectious syndromes.

Expert Rev Anti Infect Ther 2021 May 10. Epub 2021 May 10.

Policlinico Umberto I - Sapienza Università di Roma.

: New diagnostics may be useful in clinical practice, especially in contexts of high prevalence of multidrug-resistant organisms (MDRO). However, misuse of diagnostic tools may lead to increased costs and worse patient outcome. Conventional and new techniques should be appropriately positioned in diagnostic algorithms to guide an appropriate use of antimicrobial therapy. Read More

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Safe Polycationic Dendrimers as Potent Oral In Vivo Inhibitors of : A New Therapy to Take Down Tuberculosis.

Biomacromolecules 2021 May 10. Epub 2021 May 10.

Laboratoire de Chimie de Coordination du CNRS, 205 Route de Narbonne, BP 44099, 31077 Toulouse Cedex 4, France.

The long-term treatment of tuberculosis (TB) sometimes leads to nonadherence to treatment, resulting in multidrug-resistant (MDR) and extensively drug-resistant (XDR) tuberculosis. Inadequate bioavailability of the drug is the main factor for therapeutic failure, which leads to the development of drug-resistant cases. Therefore, there is an urgent need to design and develop novel antimycobacterial agents minimizing the period of treatment and reducing the propagation of resistance at the same time. Read More

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Rapid Genome Modification in Serratia marcescens Through Red Homologous Recombination.

Appl Biochem Biotechnol 2021 May 10. Epub 2021 May 10.

School of Economics and Trade, South China University of Technology, Guangzhou, 510006, China.

Despite the great potential of Serratia marcescens in industrial applications, lack of powerful genetic modification tools limits understanding of the regulatory networks of the useful metabolites and therefore restricts their mass production. To meet the urgent demand, we established a genome-editing strategy for S. marcescens based on Red recombineering in this study. Read More

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Molecular epidemiology, natural history and long-term outcomes of multi-drug resistant Enterobacterales colonization and infections among solid organ transplant recipients.

Clin Infect Dis 2021 May 10. Epub 2021 May 10.

University of Pittsburgh, Pittsburgh, Pennsylvania.

Background: Multidrug-resistant Enterobacterales (MDR-E), including carbapenem-resistant and third-generation cephalosporin-resistant Enterobacterales (CRE, CefR-E), are major pathogens following solid organ transplantation (SOT).

Methods: We prospectively studied patients who underwent lung, liver and small bowel transplant from February 2015-March 2017. Weekly peri-rectal swabs (up to 100 days post-transplant) were cultured for MDR-E. Read More

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Identification and Characterization of a Novel SXT/R391 Integrative and Conjugative Element in a Food Isolate.

Foodborne Pathog Dis 2021 May 7. Epub 2021 May 7.

College of Food Science and Engineering, Wuhan Polytechnic University, Wuhan, China.

is an opportunistic human pathogen. In this study, a novel SXT/R391 integrative and conjugative element (ICE), named ICEChnS012, was identified in the multidrug-resistant strain S012 that was isolated from retail chicken in China. Whole genome sequencing revealed that ICEPChnS012 carried 22 resistance genes including , , , , and . Read More

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Antibiotyping and Genotyping of Extensively Drug Resistant (XDR) Salmonella Spp. Isolated from Clinical Samples of Lahore, Pakistan.

J Appl Microbiol 2021 May 9. Epub 2021 May 9.

Department of Microbiology, Faculty of Life Sciences, University of Central Punjab, Lahore, Pakistan, 54590.

Aims: Antibiotic resistance is a major problem in Salmonella enterica serovar Typhi. Objective of this study was to evaluate the prevalence of XDR Salmonella among local population of Lahore and genotyping of isolates for antibiotic resistant genes.

Methods And Results: A total of 200 blood samples from suspected typhoid fever patients were collected. Read More

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Secular trends of Blood stream infections in allogeneic hematopoietic cell transplant recipients 72 hours prior to death.

Transpl Infect Dis 2021 May 9:e13631. Epub 2021 May 9.

Department of Infectious Diseases, Respiratory Institute Cleveland Clinic, Cleveland, Ohio, USA.

Introduction: Blood stream infections (BSI) frequently cause morbidity and mortality in allogeneic (allo) hematopoietic cell transplant (HCT) recipients. Characteristics of causative organisms shortly before death have not been previously described. Early treatment with antimicrobial agents targeting the recent surge in multidrug-resistant (MDR) pathogens may lead to better outcomes. Read More

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Multidrug Resistance of Strains Isolated From Bovine Feces and Carcasses in Northeast Mexico.

Front Vet Sci 2021 23;8:643802. Epub 2021 Apr 23.

Centro de Biotecnología Genómica, Instituto Politécnico Nacional, Reynosa, Mexico.

In this work, the antimicrobial resistance profile of strains ( = 248) isolated from bovine feces and carcass samples from Tamaulipas, Mexico, was evaluated. Susceptibility to 12 antibiotics conventionally used in human and veterinary treatments was determined according to Clinical and Laboratory Standards Institute guidelines. Genes encoding resistance to tetracycline ( and ), streptomycin (A), aminoglycoside (A), and β-lactamase ( and ) were investigated by PCR. Read More

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Antibacterial Activity of Lactic Acid Producing QZ1178 Against Pathogenic .

Front Vet Sci 2021 22;8:630294. Epub 2021 Apr 22.

Henan Key Laboratory of Ion-Beam Bioengineering, School of Agricultural Sciences, Zhengzhou University, Zhengzhou, China.

Lactic acid bacteria (LAB) convert carbohydrates into organic acids [mainly lactic acid (LA)], which reportedly have bactericidal activities. is a Gram-negative bacteria which infects birds, and causes significant economic losses. In this study, we investigated the antibacterial activity of the LA producing, QZ1178 from Qula (fermented food), against , using the Oxford cup method. Read More

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Molecular Epidemiology of Clinical and Colonizing Methicillin-Resistant Isolates in Companion Animals.

Front Vet Sci 2021 23;8:620491. Epub 2021 Apr 23.

School of Veterinary Science, The University of Queensland, Gatton, QLD, Australia.

In this study, we aimed to investigate the molecular epidemiology of methicillin-resistant (MRSA) and methicillin-resistant (MRSP) clinical and colonizing isolates of dogs and cats to profile contributing factors associated with their isolation. Nasal and rectal samples were collected from dogs and cats between 2015 and 2017 to identify colonizing isolates. Clinical isolates collected between 2003 and 2016 were retrieved from a Queensland university veterinary diagnostic laboratory. Read More

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Targeting RFWD2 as an Effective Strategy to Inhibit Cellular Proliferation and Overcome Drug Resistance to Proteasome Inhibitor in Multiple Myeloma.

Front Cell Dev Biol 2021 21;9:675939. Epub 2021 Apr 21.

School of Medicine & Holistic Integrative Medicine, Nanjing University of Chinese Medicine, Nanjing, China.

The potential to overcome resistance to proteasome inhibitors is greatly related with ubiquitin-proteasome system during multiple myeloma (MM) treatment process. The constitutive photomorphogenic 1 (RFWD2), referred to an E3 ubiquitin ligase, has been identified as an oncogene in multiple cancers, yet important questions on the role of RFWD2 in MM biology and treatment remain unclear. Here we demonstrated that MM patients with elevated RFWD2 expression achieved adverse outcome and drug resistance by analyzing gene expression profiling. Read More

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Comparative Utility of Genetic Determinants of Drug Resistance and Phenotypic Drug Susceptibility Profiling in Predicting Clinical Outcomes in Patients With Multidrug-Resistant .

Front Public Health 2021 22;9:663974. Epub 2021 Apr 22.

School of International Pharmaceutical Business, China Pharmaceutical University, Nanjing, China.

Programmatic management of drug-resistant tuberculosis in Ningbo, China. To assess whether data-driven genetic determinants of drug resistance patterns could outperform phenotypic drug susceptibility testing in predicting clinical meaningful outcomes among patients with multidrug-resistant tuberculosis (MDR-TB). We conducted a prospective cohort study of 104 MDR-TB patients. Read More

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Cryptotanshinone Inhibits ERα-Dependent and -Independent BCRP Oligomer Formation to Reverse Multidrug Resistance in Breast Cancer.

Front Oncol 2021 22;11:624811. Epub 2021 Apr 22.

Jiangsu Key Laboratory for Pharmacology and Safety Evaluation of Chinese Materia Medica, School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, China.

Both long-term anti-estrogen therapy and estrogen receptor-negative breast cancer contribute to drug resistance, causing poor prognosis in breast cancer patients. Breast cancer resistance protein (BCRP) plays an important role in multidrug resistance. Here, we show that cryptotanshinone (CPT), an anti-estrogen compound, inhibited the oligomer formation of BCRP on the cell membrane, thus blocking its efflux function. Read More

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Proteomic Analysis of Non-human Primate Peripheral Blood Mononuclear Cells During Infection Reveals a Role of Ezrin in Glanders Pathogenesis.

Front Microbiol 2021 22;12:625211. Epub 2021 Apr 22.

Countermeasures Division, United States Army Medical Research Institute of Infectious Diseases, Frederick, MD, United States.

, the causative agent of glanders, is a gram-negative intracellular bacterium. Depending on different routes of infection, the disease is manifested by pneumonia, septicemia, and chronic infections of the skin. poses a serious biological threat due to its ability to infect via aerosol route, resistance to multiple antibiotics and to date there are no US Food and Drug Administration (FDA) approved vaccines available. Read More

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Azithromycin Exhibits Activity Against in Chronic Rat Lung Infection Model.

Front Microbiol 2021 23;12:603151. Epub 2021 Apr 23.

Department of Microbiology, Daiichi Sankyo India Pharma Private Limited, Gurgaon, India.

forms biofilms in the lungs of chronically infected cystic fibrosis patients, which are tolerant to both the treatment of antibiotics and the host immune system. Normally, antibiotics are less effective against bacteria growing in biofilms; azithromycin has shown a potent efficacy in cystic fibrosis patients chronically infected with and improved their lung function. The present study was conducted to evaluate the effect of azithromycin on biofilm. Read More

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JD419, a Phage With a Unique Morphology and Broad Host Range.

Front Microbiol 2021 22;12:602902. Epub 2021 Apr 22.

Department of Laboratory Medicine, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Phage therapy represents a possible treatment option to cure infections caused by multidrug-resistant bacteria, including methicillin and vancomycin-resistant , to which most antibiotics have become ineffective. In the present study, we report the isolation and complete characterization of a novel phage named JD219 exhibiting a broad host range able to infect 61 of 138 clinical strains of tested, which included MRSA strains as well. The phage JD419 exhibits a unique morphology with an elongated capsid and a flexible tail. Read More

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Genomics of Environmental : Engaging Students in the Microbiology and Bioinformatics of Foodborne Pathogens.

Front Microbiol 2021 22;12:592422. Epub 2021 Apr 22.

Department of Biology, James Madison University, Harrisonburg, VA, United States.

We have developed and implemented an undergraduate microbiology course in which students isolate, characterize, and perform whole genome assembly and analysis of from stream sediments and poultry litter. In the development of the course and over three semesters, successive teams of undergraduate students collected field samples and performed enrichment and isolation techniques specific for the detection of . Eighty-eight strains were confirmed using standard microbiological methods and PCR of the gene. Read More

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