Biosens Bioelectron 2021 May 18;179:113045. Epub 2021 Feb 18.
Institute of Bioengineering, Queen Mary University of London, Mile End Road, London, E1 4NS, UK; School of Engineering and Materials Science, Queen Mary University of London, Mile End Road, London, E1 4NS, UK. Electronic address:
The ability to reversibly switch bioelectrocatalytic sensors is attractive for the design of biomonitoring platforms displaying a complex environmental response, or for the protection of biosensors. However, the retention of reversible biocatalytic properties upon multiple environmental cycles, with broad detection range, low signal-to-noise and limit of detection remains challenging. In this report, we demonstrate the precise positioning of the enzyme glucose oxidase within block-copolymer brush nanostructures, via the re-initiation of N-isopropylacrylamide (NIPAM) polymerisation from enzyme-decorated poly(dimethylaminoethyl methacrylate) (PDMAEMA) blocks. Read More