1,868 results match your criteria mucosa architecture

The effects of oral mucosa-derived heterotopic fibroblasts on cutaneous wound healing.

J Plast Reconstr Aesthet Surg 2021 Mar 25. Epub 2021 Mar 25.

Koc University School of Medicine, Department of Plastic, Reconstructive and Aesthetic Surgery, Istanbul, Turkey.

An intriguing observation that has recently found support through clinical and experimental studies is that wounds of the oral mucosa tend to display faster healing and result in less scarring than in the skin. We aimed to investigate the potential of heterotopic oral mucosal fibroblasts in cutaneous wounds while determining the main differences between wounds conditioned with either the oral mucosa or dermis-derived human fibroblasts. A total of 48 nude mice were divided into four groups: control, sham, dermal fibroblast (DF), and oral fibroblast (OF). Read More

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ZINC40099027 Promotes Gastric Mucosal Repair in Ongoing Aspirin-Associated Gastric Injury by Activating Focal Adhesion Kinase.

Cells 2021 Apr 15;10(4). Epub 2021 Apr 15.

Department of Biomedical Sciences, University of North Dakota School of Medicine & Health Sciences, Grand Forks, ND 58203, USA.

Nonsteroidal anti-inflammatory drugs cause gastric ulcers and gastritis. No drug that treats GI injury directly stimulates mucosal healing. ZINC40099027 (ZN27) activates focal adhesion kinase (FAK) and heals acute indomethacin-induced small bowel injury. Read More

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Diversion Colitis: Macro and Microscopic Findings after Probiotics Stimulation.

Biology (Basel) 2021 Apr 6;10(4). Epub 2021 Apr 6.

Department of Sociology, Social Work and Public Health, Faculty of Labour Sciences, University of Huelva, 21007 Huelva, Spain.

The use of a loop ileostomy as the defunctioning procedure of choice to protect a distal colonic anastomosis causes histological and endoscopic changes in the intestinal mucosal architecture, which have been related to chronic inflammation and changes in the microflora that consequently impact the intestinal structure and function following fecal stream diversion. The aim of this study was to evaluate the histological and endoscopic changes on the colonic mucosa in patients with diversion colitis after stimulation of the efferent loop with probiotics prior to closure of the protective ileostomy. A prospective, randomized, double-blind, controlled study was designed. Read More

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High Anti- IgG Antibody Levels Are Associated With Severity of Mucosal Leishmaniasis.

Front Cell Infect Microbiol 2021 9;11:652956. Epub 2021 Apr 9.

Serviço de Imunologia, Hospital Universitário Prof. Edgard Santos, Universidade Federal da Bahia, Salvador, Brazil.

Background: Mucosal leishmaniasis (ML), the most inflammatory form of tegumentary leishmaniasis, is predominantly caused by . The disease is characterized by the development of lesions, mainly in the nasal mucosa. An exacerbated inflammatory response has been associated with the presence of destructive and disfiguring lesions, with stages of severity ranging from small nodulations to the complete destruction of the nasal pyramid architecture. Read More

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Histopathological effects on stomach, liver and kidney associated with Cedrus deodara root oil in ulcer induced rats (Wistar strain).

Pak J Pharm Sci 2020 Nov;33(6):2483-2488

Department of Pharmacology, Baqai Institute of Pharmaceutical Sciences, Baqai Medical University, 51 Deh Tor, Gadap Road, Near Toll Plaza, Super Highway, Karachi, Pakistan.

Cedrus deodara have been used traditionally in ayurvedic system against peptic ulcer. Present work is concerned with the determination of histopathological effects in ethanol induced ulcer on rats (Wistar Strain) treated with Cedrus deodara root oil at a dose of 200mg/kg and comparison of its antiulcer activity against control, positive control and standard anti-ulcer drug (Omeprazole). The aim was to find out the antiulcer effect of Cedrus deodara root oil and to observe histopathology of liver, kidney as well. Read More

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November 2020

Interplay of Biochemical, Genetic, and Immunohistochemical Factors in the Etio-Pathogenesis of Gastric Ulcer in Rats: A Comparative Study of the Effect of Pomegranate Loaded Nanoparticles Versus Pomegranate Peel Extract.

Front Physiol 2021 23;12:649462. Epub 2021 Mar 23.

Medical Physiology Department, Faculty of Medicine, Assiut University, Assiut, Egypt.

Background: Few data are available about the role of herbal extract loaded nanoparticles as an alternative safe medicine for the management of a gastric ulcer.

Aim: This work is targeted at exploring the physiological effects of pomegranate loaded nanoparticles (PLN) against an indomethacin IND-induced gastric ulcer and comparing the results with traditional pomegranate peel extract (PPE).

Methods: Twenty-four rats were equally distributed into four groups: control, IND-treated, PLN-treated, and PPE-treated groups. Read More

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Extracellular vesicles isolated from milk can improve gut barrier dysfunction induced by malnutrition.

Sci Rep 2021 Apr 7;11(1):7635. Epub 2021 Apr 7.

Translational Medicine Program, Hospital for Sick Children, Toronto, ON, Canada.

Malnutrition impacts approximately 50 million children worldwide and is linked to 45% of global mortality in children below the age of five. Severe acute malnutrition (SAM) is associated with intestinal barrier breakdown and epithelial atrophy. Extracellular vesicles including exosomes (EVs; 30-150 nm) can travel to distant target cells through biofluids including milk. Read More

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Pseudo-Placentational Endometrial Hyperplasia in the Bitch: Case Series.

Animals (Basel) 2021 Mar 6;11(3). Epub 2021 Mar 6.

Department of Veterinary Medicine and Animal Production, University of Naples, Federico II, 80137 Naples, Italy.

Canine pseudo-placentational endometrial hyperplasia differs from the classical form of cystic endometrial hyperplasia for the well-organized tissue architecture resembling the canine placenta. After the discovery, it has been inconstantly reported. The present work reports the clinicopathological details of six spontaneous cases retrieved retrospectively from a large database. Read More

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DNA methylation architecture of the ACE2 gene in nasal cells of children.

Sci Rep 2021 03 29;11(1):7107. Epub 2021 Mar 29.

Division of Chronic Disease Research Across the Lifecourse, Department of Population Medicine, Harvard Medical School and Harvard Pilgrim Health Care Institute, Boston, MA, USA.

Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has led to the global coronavirus disease 2019 (COVID-19) pandemic. SARS-CoV-2 enters cells via angiotensin-Converting Enzyme 2 (ACE2) receptors, highly expressed in nasal epithelium with parallel high infectivity. The nasal epigenome is in direct contact with the environment and could explain COVID-19 disparities by reflecting social and environmental influences on ACE2 regulation. Read More

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Papillary immature squamous metaplasia of the anal canal: a rare but probably underdiagnosed entity.

Histopathology 2021 Mar 23. Epub 2021 Mar 23.

Department of Pathology, Institut Jules Bordet, Université Libre de Bruxelles (ULB).

A 63-years-old man presented with a condylomatous hemicirconferential exophytic lesion of the anal canal. He had a history of anal squamous cell carcinoma, cT2N0M0, treated by radiochemotherapy and some recurrent condylomata treated by electrocoagulation. Given this background as well as the morbidity of an abdominoperineal amputation, a mucosectomy was performed. Read More

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Targeted next-generation sequencing supports serrated epithelial change as an early precursor to inflammatory bowel disease-associated colorectal neoplasia.

Hum Pathol 2021 Mar 13;112:9-19. Epub 2021 Mar 13.

Department of Pathology, Johns Hopkins Hospital, Baltimore, MD, 21205, USA; Department of Pathology, University of Miami Miller School of Medicine, Miami, FL, 33136, USA. Electronic address:

Serrated epithelial change (SEC) manifests in patients with long-standing inflammatory bowel disease (IBD) and is characterized by disorganized crypt architecture, irregular serrations, and goblet cell-rich epithelium. The serrated nature of SEC is reminiscent of serrated colorectal polyps, which frequently harbor KRAS/BRAF mutations. SEC is, however, not only histologically distinct from sporadic serrated polyps but also associated with colorectal neoplasia. Read More

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Dequalinium Chloride Effectively Disrupts Bacterial Vaginosis (BV) spp. Biofilms.

Pathogens 2021 Feb 25;10(3). Epub 2021 Feb 25.

CICS-UBI-Health Sciences Research Center, University of Beira Interior, 6200-506 Covilhã, Portugal.

Bacterial vaginosis (BV) is the most frequent vaginal infection worldwide. It is caused by the overgrowth of anaerobic vaginal pathogens such as spp. BV has been associated with the occurrence of dense multispecies biofilms on the vaginal mucosa. Read More

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February 2021

Secretes Outer Membrane Vesicles and Promotes Intestinal Inflammation.

mBio 2021 03 2;12(2). Epub 2021 Mar 2.

Department of Pathology and Immunology, Baylor College of Medicine, Houston, Texas, USA.

Multiple studies have implicated microbes in the development of inflammation, but the mechanisms remain unknown. Bacteria in the genus have been identified in the intestinal mucosa of patients with digestive diseases; thus, we hypothesized that promotes intestinal inflammation. The addition of >50 kDa conditioned media, which contain outer membrane vesicles (OMVs), to colonic epithelial cells stimulated secretion of the proinflammatory cytokines interleukin-8 (IL-8) and tumor necrosis factor (TNF). Read More

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IFT46 Expression in the Nasal Mucosa of Primary Ciliary Dyskinesia Patients: Preliminary Study.

Allergy Rhinol (Providence) 2021 Jan-Dec;12:2152656721989288. Epub 2021 Feb 11.

Pathology Department, Faculty of Medicine, University of Valencia, Valencia, Spain.

Background: Primary ciliary dyskinesia (PCD) is characterised by an imbalance in mucociliary clearance leading to chronic respiratory infections. Cilia length is considered to be a contributing factor in cilia movement. Recently, protein has been related to cilia length. Read More

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February 2021

MicroRNAs overexpressed in Crohn's disease and their interactions with mechanisms of epigenetic regulation explain novel aspects of Crohn's disease pathogenesis.

Clin Epigenetics 2021 Feb 18;13(1):39. Epub 2021 Feb 18.

Facultad de Ciencias Básicas y Biomédicas, Universidad Simon Bolivar, 080001, Barranquilla, Colombia.

Background: In this review, we were interested to identify the wide universe of enzymes associated with epigenetic modifications, whose gene expression is regulated by miRNAs with a high relative abundance in Crohn's disease (CD) affected tissues, with the aim to determine their impact in the pathogenesis and evolution of the disease.

Methods: We used HMDD and Bibliometrix R-package in order to identify the miRNAs overexpressed in CD. The identified enzymes associated with epigenetic mechanisms and post-translational modifications, regulated by miRNAs upregulated in CD, were analyzed using String v11 database. Read More

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February 2021

Intraoperative free margins assessment of oropharyngeal squamous cell carcinoma with confocal laser endomicroscopy: a pilot study.

Eur Arch Otorhinolaryngol 2021 Feb 13. Epub 2021 Feb 13.

Department of Otorhinolaryngology, Head and Neck Surgery, University Hospital, University of Erlangen-Nuremberg, Germany, Waldstrasse 1, 91054, Erlangen, Germany.

Purpose: This pilot study aimed to assess the feasibility of intraoperative assessment of safe margins with confocal laser endomicroscopy (CLE) during oropharyngeal squamous cell carcinoma (OPSCC) surgery.

Methods: We included five consecutive patients confirmed OPSCC and planned tumor resection in September and October 2020. Healthy appearing mucosa in the marginal zone, and the tumor margin, were examined with CLE and biopsy during tumor resection. Read More

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February 2021

Maxillary Sinus Lobular Capillary Hemangioma in a 15-Year-Old Boy.

Ear Nose Throat J 2021 Feb 9:145561321993597. Epub 2021 Feb 9.

Department of Otorhinolaryngology, 112404Military Medical Academy, Faculty of Medicine, University of Defence, Belgrade, Serbia.

Lobular capillary hemangioma (LCH) is a benign capillary proliferation with a microscopically distinctive lobular architecture, composed of small to medium-sized vessels of capillary type. Rarely, it originates from the mucosa of the nasal cavity, most frequently from the anterior nasal septum, turbinates, and nasal vestibule. Etiology is unclear, but previous investigations suggest that this lesion is associated with injury, hormonal factors, some viral infections, and therapy with some drugs. Read More

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February 2021

Dynamic diagnosis of early gastric cancer with microvascular blood flow rate using magnifying endoscopy (with video): A pilot study.

J Gastroenterol Hepatol 2021 Feb 3. Epub 2021 Feb 3.

Department of Gastroenterology, Juntendo University School of Medicine, Tokyo, Japan.

Background And Aim: Magnifying endoscopy (ME) diagnostic algorithm for early gastric cancer (EGC) relies on qualitative features such as microvascular (MV) architecture and microsurface structure; however, it is a "static" diagnostic algorithm that uses still images. ME can visualize red blood cell flow within subepithelial microvessels in real time. Here, we evaluated the utility of using the MV blood flow rate in combination with ME for the diagnosis of EGC as a retrospective study. Read More

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February 2021

Constitutive activation of mitogen-activated protein kinase kinase (MEK1) in ileal enterocytes leads to dysplasia and a predisposition to cancer.

Am J Physiol Gastrointest Liver Physiol 2021 03 20;320(3):G366-G379. Epub 2021 Jan 20.

Department of Molecular and Cellular Biology, Dan L. Duncan Comprehensive Cancer Center, Houston, Texas.

Activation of mitogen-activated protein kinases (MAPKs) is a key factor in the pathogenesis of cancer, although the specific role of mitogen-activated protein kinase kinase (MEK1) is not well understood. Villin promoter-driven Cre expression was used to excise a floxed stop cassette from a phosphomimetically constitutively activated MEK1 (caMEK1) expression construct in the intestine of C57BL/6 mice. Zygosity status of caMEK1 afforded assessment of the dose dependence of the effect. Read More

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Murine Intrarectal Instillation of Purified Recombinant Toxins Enables Mechanistic Studies of Pathogenesis.

Infect Immun 2021 Mar 17;89(4). Epub 2021 Mar 17.

Epithelial Biology Center, Vanderbilt University School of Medicine, Nashville, Tennessee, USA

is linked to nearly 225,000 antibiotic-associated diarrheal infections and almost 13,000 deaths per year in the United States. Pathogenic strains of produce toxin A (TcdA) and toxin B (TcdB), which can directly kill cells and induce an inflammatory response in the colonic mucosa. Hirota et al. Read More

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Feasibility of intraoperative assessment of safe surgical margins during laryngectomy with confocal laser endomicroscopy: A pilot study.

Auris Nasus Larynx 2021 Aug 16;48(4):764-769. Epub 2021 Jan 16.

Department of Otorhinolaryngology, Head and Neck Surgery, Friedrich-Alexander-Universität Erlangen-Nürnberg, University Hospital, Waldstraße 1, 91054 Erlangen, Germany. Electronic address:

Objective: This pilot study aimed to assess the feasibility of intraoperative assessment of safe margins with Confocal Laser Endomicroscopy (CLE) during planned partial or total laryngectomy.

Methods: Eight patients with confirmed larynx squamous cell carcinoma (SCC) and planned partial or total laryngectomy were included in this study in March 2020. Two head and neck surgeons and one pathologist were asked to classify carcinoma or healthy epithelium in a sample of 94 representative sequences (5. Read More

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Quantification of Accidental Gluten Contamination in the Diet of Children with Treated Celiac Disease.

Nutrients 2021 Jan 9;13(1). Epub 2021 Jan 9.

Division of Pediatrics, DISCO Department, Polytechnic University of Marche, 60123 Ancona, Italy.

A strict gluten-free diet is extremely difficult to maintain. Protracted ingestion of gluten traces (>10 mg/day) is sufficient to cause significant damage in the architecture of the small intestinal mucosa in patients on treatment for celiac disease. The aim of this study was to directly measure the level of contaminating gluten in the daily diet of celiac children following a gluten-free diet. Read More

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January 2021

Review of Dermoscopy and Reflectance Confocal Microscopy Features of the Mucosal Melanoma.

Diagnostics (Basel) 2021 Jan 8;11(1). Epub 2021 Jan 8.

Department of Medical, Surgical and Neurological Science, Dermatology Section, University of Siena, S. Maria alle Scotte Hospital, 53100 Siena, Italy.

Mucosal melanoma is a rare tumor with aggressive biological behavior and poor prognosis. Diagnosis is often performed at an advanced stage when the lesions become symptomatic. Although dermoscopy and reflectance confocal microscopy (RCM) are widely used techniques for the diagnosis of cutaneous tumors, their use for mucosal lesions is not well established, probably because the latter are rarer. Read More

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January 2021

Safety and Feasibility of a Novel Esophageal Balloon for Circumferential Cytologic Sampling.

Ann Otol Rhinol Laryngol 2021 Jun 14;130(6):585-590. Epub 2020 Oct 14.

Aero-Di-Namics, New York, New York, USA.

Objectives: A prior publication introduced the Strome-Blitzer balloon's ability to obtain circumferential esophageal cytologic sampling. This GLP study was requisite for FDA approval to determine if equivalent cell capture and cellularity was observed with the balloon compared to surface sampling brushes and to determine the balloon's usability for naive otolaryngologists.

Methods: Three naïve users tested the Hobbs brush and Strome-Blitzer balloon on 4 Yorkshire swine. Read More

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Petroleum extract of alleviates nasal symptoms by regulating the Th1-Th2 cytokine balance in a mouse model of Allergic Rhinitis.

Int J Med Sci 2021 1;18(2):555-563. Epub 2021 Jan 1.

Shanxi Key Laboratory of Otorhinolaryngology Head and Neck Cancer, First Hospital of Shanxi Medical University, Taiyuan 030001, Shanxi, China.

is a traditional Chinese medicine that has long been used to treat allergies. In this study, we aimed to investigate the effect of a petroleum extract of (PEFF) in a mouse model of allergic rhinitis (AR) and to explore the underlying molecular mechanisms of action. An animal model of AR was established by sensitization and challenge of BALB/c mice with ovalbumin (OVA). Read More

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January 2021

The role of histopathology in the diagnosis and management of coeliac disease and other malabsorptive conditions.

Histopathology 2021 Jan;78(1):88-105

Envoi Specialist Pathologists, University of Queensland, Brisbane, Queensland, Australia.

Most absorption of nutrients takes place in the proximal small intestine, and the most common disorders leading to malabsorption are associated with a morphological abnormality in the duodenal mucosa that is appreciable in histological sections of biopsy specimens. Coeliac disease is the most well-known example, causing intraepithelial lymphocytosis, inflammation and villous atrophy in the duodenum. Remarkably similar inflammatory changes can be induced by other processes, including medications, e. Read More

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January 2021

Primary Extracellular Matrix Enables Long-Term Cultivation of Human Tumor Oral Mucosa Models.

Front Bioeng Biotechnol 2020 4;8:579896. Epub 2020 Dec 4.

Institute of Pharmacy (Pharmacology and Toxicology), Freie Universität Berlin, Berlin, Germany.

3D tumor models clearly outperform 2D cell cultures in recapitulating tissue architecture and drug response. However, their potential in understanding treatment efficacy and resistance development should be better exploited if also long-term effects of treatment could be assessed . The main disadvantages of the matrices commonly used for culture are their limited cultivation time and the low comparability with patient-specific matrix properties. Read More

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December 2020

Probe-based confocal laser endomicroscopy for in vivo assessment of histological healing in ulcerative colitis: development and validation of the ENHANCE index.

J Crohns Colitis 2020 Dec 18. Epub 2020 Dec 18.

Department of Gastroenterology, Inserm U1256 NGERE, University Hospital of Nancy-Brabois, Lorraine University, Vandœuvre-lès-Nancy, France.

Background And Aims: Histological healing may represent the ultimate therapeutic goal in ulcerative colitis (UC), but it requires biopsies. Our aim was to develop a non-invasive index able to assess histological disease activity in ulcerative colitis using probe based confocal laser endomicroscopy (pCLE).

Methods: One hundred patients with quiescent UC were prospectively included in 5 French centres. Read More

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December 2020

Saccharomyces boulardii as therapeutic alternative in experimental giardiasis.

J Appl Microbiol 2020 Dec 2. Epub 2020 Dec 2.

Department of Parasitology, Institute of Biological Sciences, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.

Aims: The objective of this study was to evaluate the effect of treatment with the probiotic Saccharomyces boulardii with or without metronidazole in experimental giardiasis.

Methods And Results: The effect of treatment with S. boulardii with or without metronidazole on the intestinal mucosa, the antioxidant defense system, and the parasitic load was determined in experimental giardiasis. Read More

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December 2020

A bioengineering perspective on modelling the intestinal epithelial physiology in vitro.

Nat Commun 2020 12 7;11(1):6244. Epub 2020 Dec 7.

BRIC - Biotech Research and Innovation Centre, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.

The small intestine is a specialised organ, essential for nutrient digestion and absorption. It is lined with a complex epithelial cell layer. Intestinal epithelial cells can be cultured in three-dimensional (3D) scaffolds as self-organising entities with distinct domains containing stem cells and differentiated cells. Read More

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December 2020