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Bioresour Technol 2009 Jan 13;100(2):991-5. Epub 2008 Aug 13.

Fermentation Technology and Bioengineering Department, Central Food Technological Research Institute, Mysore 570 020, Karnataka, India.

Comparison of various pretreatments such as mild alkali/acid treatments and pressure cooking of corncob to expose its lignin-saccharide complex has been carried out to enhance enzymatic hydrolysis of xylan to xylooligosaccharides (XOS). Scanning electron micrographs of lignin-saccharide complex of native and pretreated corncob powder showed that the complex was greatly altered during alkali pretreatment. Hydrolysis of alkali pretreated corncob powder using a commercial endoxylanase produced 81+/-1. Read More

J Agric Food Chem 2008 Jun 20;56(11):3981-8. Epub 2008 May 20.

Fermentation Technology and Bioengineering Department, Central Food Technological Research Institute, Mysore, Karnataka, India.

Eight different fungi were cultivated in a peptone-yeast extract medium containing 1% oat spelt xylan (OSX) to evaluate endo-1,4-beta-xylanase secretion for xylooligosaccharide (XOS) production. Aspergillus oryzae MTCC 5154, Aspergillus flavus , Aspergillus niger , and Aspergillus ochraceus showed significant titers of endoxylanases, which were further used for the production of XOS from birch wood xylan (BWX). A. Read More

Carbohydr Res 2008 Jan 23;343(1):56-66. Epub 2007 Oct 23.

Department of Fermentation Technology and Bioengineering, Central Food Technological Research Institute, Mysore 570 020, India.

Fructooligosaccharides (FOSs) were prepared from sucrose using fungal fructosyl transferase (FTase) obtained from Aspergillus oryzae MTCC 5154. The resulting mixture consisted of glucose (28-30%), sucrose (18-20%) and fructooligosaccharides (50-54%) as indicated by HPLC analysis. Identification of oligomers present in the mixture of fructooligosaccharides was carried out using NMR spectroscopy and LC-MS. Read More