254 results match your criteria mtb proliferation

The Cholinergic System Contributes to the Immunopathological Progression of Experimental Pulmonary Tuberculosis.

Front Immunol 2020 18;11:581911. Epub 2021 Feb 18.

Division of Experimental Pathology, Department of Pathology, National Institute of Medical Sciences and Nutrition Salvador Zubirán, México City, Mexico.

The cholinergic system is present in both bacteria and mammals and regulates inflammation during bacterial respiratory infections through neuronal and non-neuronal production of acetylcholine (ACh) and its receptors. However, the presence of this system during the immunopathogenesis of pulmonary tuberculosis (TB) and in its causative agent () has not been studied. Therefore, we used an experimental model of progressive pulmonary TB in BALB/c mice to quantify pulmonary ACh using high-performance liquid chromatography during the course of the disease. Read More

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February 2021

Immunological characterization of chimeras of high specificity antigens from Mycobacterium tuberculosis H37Rv.

Tuberculosis (Edinb) 2021 Mar 28;127:102054. Epub 2021 Jan 28.

Molecular and Structural Biology Division, CSIR-Central Drug Research Institute, Lucknow, 226031, India; Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, 201002, India. Electronic address:

Tuberculosis remains a serious global health problem. BCG is the only prophylactic TB vaccine and it shows variable protective efficacy. Chimeric protein subunit vaccines hold great potential as stand-alone vaccines or heterologous BCG prime boosters. Read More

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Zn Intoxication of Mycobacterium marinum during Dictyostelium discoideum Infection Is Counteracted by Induction of the Pathogen Zn Exporter CtpC.

mBio 2021 02 2;12(1). Epub 2021 Feb 2.

Department of Biochemistry, Faculty of Science, University of Geneva, Geneva, Switzerland

Macrophages use diverse strategies to restrict intracellular pathogens, including either depriving the bacteria of (micro)nutrients such as transition metals or intoxicating them via metal accumulation. Little is known about the chemical warfare between , a close relative of (Mtb), and its hosts. We use the professional phagocyte to investigate the role of Zn during infection. Read More

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February 2021

Increased Frequency of Memory CD4+ T-Cell Responses in Individuals With Previously Treated Extrapulmonary Tuberculosis.

Front Immunol 2020 17;11:605338. Epub 2020 Dec 17.

Division of Infectious Diseases, Department of Medicine, Vanderbilt University Medical Center, Nashville, TN, United States.

Extrapulmonary TB (EPTB) occurs with increased frequency in persons with underlying immunodeficiency. Even after recovery from acute illness, differences in immune phenotype and activation persist. Studies defining characteristics of immune responses after recovery from extrapulmonary TB may provide insights into factors that increase TB risk. Read More

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December 2020

LncRNA NEAT1 participates in inflammatory response in macrophages infected by mycobacterium tuberculosis through targeted regulation of miR-377-3p.

Microb Pathog 2021 Jan 30;150:104674. Epub 2020 Nov 30.

Shanghai Clinical Research Center for Tuberculosis, Shanghai Key Laboratory of Tuberculosis, Shanghai Pulmonary Hospital, Tongji University School of Medicine, Shanghai, 200433, China. Electronic address:

Background Tuberculosis (TB) is a very serious public health problem in the world at present. The incidence rate is rising continuously. Once it develops to the middle and late stage, it can cause serious tissue damage and necrosis, directly threatening the life and health of patients. Read More

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January 2021

Lung gene expression signatures suggest pathogenic links and molecular markers for pulmonary tuberculosis, adenocarcinoma and sarcoidosis.

Commun Biol 2020 Oct 23;3(1):604. Epub 2020 Oct 23.

CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, 100101, Beijing, China.

Previous reports have suggested a link between pulmonary tuberculosis (TB), which is caused by Mycobacterium tuberculosis (Mtb), and the development of lung adenocarcinoma (LUAD) and sarcoidosis. Furthermore, these lung diseases share certain clinical similarities that can challenge differential diagnosis in some cases. Here, through comparison of lung transcriptome-derived molecular signatures of TB, LUAD and sarcoidosis patients, we identify certain shared disease-related expression patterns. Read More

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October 2020

Low-dose 2-deoxy glucose stabilises tolerogenic dendritic cells and generates potent in vivo immunosuppressive effects.

Cell Mol Life Sci 2021 Mar 19;78(6):2857-2876. Epub 2020 Oct 19.

Institute of Medical Sciences, School of Medicine, Medical Sciences and Nutrition, Foresterhill, University of Aberdeen, Aberdeen, AB25 2ZD, Scotland, UK.

Cell therapies for autoimmune diseases using tolerogenic dendritic cells (tolDC) have been promisingly explored. A major stumbling block has been generating stable tolDC, with low risk of converting to mature immunogenic DC (mDC), exacerbating disease. mDC induction involves a metabolic shift to lactate production from oxidative phosphorylation (OXPHOS) and β-oxidation, the homeostatic energy source for resting DC. Read More

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Distinct epigenetic regulation in patients with multidrug-resistant TB-HIV co-infection and uninfected individuals.

Mutat Res 2020 May - Dec;821:111724. Epub 2020 Oct 13.

Department of Clinical Microbiology and Infectious Diseases, School of Pathology, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, 2193, South Africa; Infectious Diseases, Charlotte Maxeke Johannesburg Academic Hospital, National Health Laboratory Service, Johannesburg, 2193, South Africa.

Background: Mycobacterium tuberculosis (Mtb) is an airborne pathogenic microorganism that causes tuberculosis (TB). This pathogen invades lung tissues causing pulmonary infections and disseminates into other host organs. The Bacillus Calmette-Guérin (BCG) vaccine is employed to provide immune protection against TB; however, its efficacy is dependent on the age, immune status and geographic location of vaccinated individuals. Read More

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December 2020

Adults from Kisumu, Kenya have robust γδ T cell responses to Schistosoma mansoni, which are modulated by tuberculosis.

PLoS Negl Trop Dis 2020 10 12;14(10):e0008764. Epub 2020 Oct 12.

Emory Vaccine Center, Emory University, Atlanta, Georgia, United States of America.

Schistosoma mansoni (SM) is a parasitic helminth that infects over 200 million people and causes severe morbidity. It undergoes a multi-stage life cycle in human hosts and as such stimulates a stage-specific immune response. The human T cell response to SM is complex and varies throughout the life cycle of SM. Read More

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October 2020

Calcium Pump CtpF Modulates the Autophagosome in an mTOR-Dependent Manner.

Front Cell Infect Microbiol 2020 16;10:461. Epub 2020 Sep 16.

Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore, India.

Calcium is a very important second messenger, whose concentration in various cellular compartments is under tight regulation. A disturbance in the levels of calcium in these compartments can play havoc in the cell, as it regulates various cellular processes by direct or indirect mechanisms. Here, we have investigated the functional importance of a calcium transporting P2A ATPase, CtpF of (Mtb) in the pathogen's interaction with the host. Read More

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September 2020

LncRNA MEG3 control Mycobacterium Tuberculosis infection via controlled MiR-145-5p expression and modulation of macrophages proliferation.

Microb Pathog 2020 Dec 6;149:104550. Epub 2020 Oct 6.

Clinic and Research Center of Tuberculosis, Shanghai Key Laboratory of Tuberculosis, Shanghai Pulmonary Hospital, Tongji University School of Medicine, No.507 Zhengmin Road, Yangpu District, Shanghai, 200433, China. Electronic address:

Background: Mycobacterium tuberculosis (Mtb) is an intractable pathogen for humans to overcome. While as an important part of innate immunity, macrophages play an important role in resisting foreign pathogenic microorganisms, and it has been proved that there is a close relationship between macrophages and Mtb. In recent years, with the in-depth study of LncRNA, there have been crucial breakthroughs in the diagnosis and treatment of a number of diseases, and understanding the impact of LncRNA on Mtb may also be conducive to providing new therapeutic targets for tuberculosis prevention and treatment in the future. Read More

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December 2020

[Butyrophilin 3A1 (BTN3A1) enhances activation and proliferation of human peripheral blood Vγ9Vδ2 T cells induced by MTB-HAg].

Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi 2020 Aug;36(8):680-686

Department of Immunology, Bengbu Medical College, Anhui Key Laboratory of Infection and Immunity, Bengbu Medical College, Bengbu 233000, China. *Corresponding authors, E-mail:

Objective To investigate the role of butyrophilin 3A1 (BTN3A1) in the activation and proliferation of human peripheral blood Vγ9Vδ2 T cells induced by M. tuberculosis heat resistant antigen (MTB-HAg). Methods Human peripheral blood mononuclear cells (PBMCs) were treated with BTN3A1 blocking antibody for 3 hours and then stimulated with MTB-HAg or phosphoantigen (PAg). Read More

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Design, synthesis and antimycobacterial activity of new benzothiazinones inspired by rifampicin/rifapentine.

Bioorg Chem 2020 09 22;102:104135. Epub 2020 Jul 22.

Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China. Electronic address:

A series of novel benzothiazinone derivatives containing a N-((methylene)amino)piperazine moiety, inspired by rifampicin/rifapentine, were designed and synthesized. Seven compounds 1a and 1e-j show excellent in vitro activity against both drug-sensitive MTB strain H37Rv and drug-resistant clinical isolates (MIC: <0.029-0. Read More

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September 2020

Host sirtuin 2 as an immunotherapeutic target against tuberculosis.

Elife 2020 07 22;9. Epub 2020 Jul 22.

Signal Transduction Laboratory 1, National Institute of Immunology, Aruna Asaf Ali Marg, New Delhi, India.

() employs plethora of mechanisms to hijack the host defence machinery for its successful survival, proliferation and persistence. Here, we show that upregulates one of the key epigenetic modulators, NAD+ dependent histone deacetylase Sirtuin 2 (SIRT2), which upon infection translocate to the nucleus and deacetylates histone H3K18, thus modulating the host transcriptome leading to enhanced macrophage activation. Furthermore, in specific T cells, SIRT2 deacetylates NFκB-p65 at K310 to modulate T helper cell differentiation. Read More

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Ornithine-A urea cycle metabolite enhances autophagy and controls Mycobacterium tuberculosis infection.

Nat Commun 2020 07 15;11(1):3535. Epub 2020 Jul 15.

Department of Pulmonary Immunology, Center for Biomedical Research, University of Texas Health Center, Tyler, TX, 75708, USA.

Macrophages are professional phagocytes known to play a vital role in controlling Mycobacterium tuberculosis (Mtb) infection and disease progression. Here we compare Mtb growth in mouse alveolar (AMs), peritoneal (PMs), and liver (Kupffer cells; KCs) macrophages and in bone marrow-derived monocytes (BDMs). KCs restrict Mtb growth more efficiently than all other macrophages and monocytes despite equivalent infections through enhanced autophagy. Read More

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A novel biosafety level 2 compliant tuberculosis infection model using a ΔΔ double auxotroph of H37Rv and .

Virulence 2020 12;11(1):811-824

Section of Paediatric Infectious Disease, Department of Infectious Disease, Imperial College London , London, UK.

Mammalian infection models have contributed significantly to our understanding of the host-mycobacterial interaction, revealing potential mechanisms and targets for novel antimycobacterial therapeutics. However, the use of conventional mammalian models such as mice, are typically expensive, high maintenance, require specialized animal housing, and are ethically regulated. Furthermore, research using (MTB), is inherently difficult as work needs to be carried out at biosafety level 3 (BSL3). Read More

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December 2020

Gut Dysbiosis Thwarts the Efficacy of Vaccine Against .

Front Immunol 2020 19;11:726. Epub 2020 May 19.

CSIR-Institute of Microbial Technology, Chandigarh, India.

The generation of enduring protective immunity by vaccines is of utmost importance. Intriguingly, there is considerable variation in the efficacy of vaccines amongst individuals. Various studies have shown that normal flora of gastrointestinal tract plays a vital role in maintaining host homeostasis and immunity. Read More

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Identification of Substituted Amino Acid Hydrazides as Novel Anti-Tubercular Agents, Using a Scaffold Hopping Approach.

Molecules 2020 May 21;25(10). Epub 2020 May 21.

Biosciences Institute, Faculty of Medical Sciences, Newcastle University, Newcastle upon Tyne NE2 4HH, UK.

Discovery and development of new therapeutic options for the treatment of () infection, particularly drug-resistant strains, are urgently required to tackle the global burden of this disease. Herein, we reported the synthesis of a novel series of N-substituted amino acid hydrazides, utilising a scaffold hopping approach within a library of anti-tubercular agents. Efficacy and selectivity were evaluated against three strains of (wild-type, isoniazid-resistant and rifampicin-resistant), and cytotoxicity against macrophages . Read More

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Mycobacterium tuberculosis Rv3034c regulates mTORC1 and PPAR-γ dependant pexophagy mechanism to control redox levels in macrophages.

Cell Microbiol 2020 09 10;22(9):e13214. Epub 2020 Jun 10.

School of Biotechnology, KIIT (Deemed to be University), Bhubaneswar, India.

Mycobacterium tuberculosis survives inside the macrophages by employing several host immune evasion strategies. Here, we reported a novel mechanism in which M. tuberculosis acetyltransferase, encoded by Rv3034c, induces peroxisome homeostasis to regulate host oxidative stress levels to facilitate intracellular mycobacterial infection. Read More

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September 2020

Flagella and Swimming Behavior of Marine Magnetotactic Bacteria.

Biomolecules 2020 03 16;10(3). Epub 2020 Mar 16.

International Associated Laboratory of Evolution and Development of Magnetotactic Multicellular Organisms, CNRS-Marseille F-13402 France/ CAS-Sanya 572000, China.

Marine environments are generally characterized by low bulk concentrations of nutrients that are susceptible to steady or intermittent motion driven by currents and local turbulence. Marine bacteria have therefore developed strategies, such as very fast-swimming and the exploitation of multiple directional sensing-response systems in order to efficiently migrate towards favorable places in nutrient gradients. The magnetotactic bacteria (MTB) even utilize Earth's magnetic field to facilitate downward swimming into the oxic-anoxic interface, which is the most favorable place for their persistence and proliferation, in chemically stratified sediments or water columns. Read More

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Lymphocyte responses to Mycobacterium tuberculosis and Mycobacterium bovis are similar between BCG-vaccinated patients with cystic fibrosis and healthy controls.

J Cyst Fibros 2020 07 13;19(4):575-579. Epub 2020 Feb 13.

Center for Investigation in Pediatrics, School of Medical Sciences, University of Campinas, Campinas/SP, Brazil. Electronic address:

Background: The low rate of nontuberculous mycobacteria (NTM) among Brazilian patients with cystic fibrosis (CF) may be due to cross-reactive Bacille Calmette-Guérin (BCG) vaccination. In the present pilot study, we aimed to compare the lymphocyte responses against Mycobacterium tuberculosis(Mtb) and Mycobacterium bovis (BCG) in BCG-vaccinated CF patients and healthy controls.

Methods: The lymphocyte responses of CF patients (n = 10) and healthy controls (n = 10) were assessed in terms of lymphocyte proliferation index (LPI), using flow cytometry. Read More

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Immune responses to Mycobacterium tuberculosis membrane-associated antigens including alpha crystallin can potentially discriminate between latent infection and active tuberculosis disease.

PLoS One 2020 31;15(1):e0228359. Epub 2020 Jan 31.

Department of Clinical Immunology & Rheumatology, Sanjay Gandhi Postgraduate Institute of Medical Sciences, Lucknow, India.

Changes in expression of membrane antigens may accompany the transition of Mycobacterium tuberculosis (Mtb) from 'dormant' to 'active' states. We have determined whether antibody and T cell responses to Mtb membrane (MtM)-associated antigens, especially the latency-induced protein alpha crystallin (Acr), can discriminate between latent tuberculosis infection (LTBI) and active TB (ATB) disease. Study subjects comprised a previously described cohort of healthcare workers (HCWs, n = 43) and smear-positive ATB patients (n = 10). Read More

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Mycobacterium tuberculosis, antimicrobials, immunity, and lung-gut microbiota crosstalk: current updates and emerging advances.

Ann N Y Acad Sci 2020 05 28;1467(1):21-47. Epub 2020 Jan 28.

Molecular Mycobacteriology Laboratory, Department of Medical Microbiology, School of Medicine, Faculty of Health Sciences, University of Pretoria, Pretoria, South Africa.

Increasingly, gut microbiota distortions are being implicated in the pathogenesis of several infectious and noninfectious diseases. Specifically, in the absence of an eubiotic microbiota, mice are more prone to colonization and infection by Mycobacterium tuberculosis (Mtb). In this qualitative analysis, the following were observed: (1) antimicrobials cause long-term gut microbiota perturbations; (2) Mtb causes limited and transient disturbances to the lung-gut microbiota; (3) pathogens (e. Read More

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Mycobacterium tuberculosis Limits Host Glycolysis and IL-1β by Restriction of PFK-M via MicroRNA-21.

Cell Rep 2020 01;30(1):124-136.e4

School of Biochemistry and Immunology, Trinity College, Dublin 2, Ireland; Conway Institute, University College Dublin, Dublin, Ireland. Electronic address:

Increased glycolytic metabolism recently emerged as an essential process driving host defense against Mycobacterium tuberculosis (Mtb), but little is known about how this process is regulated during infection. Here, we observe repression of host glycolysis in Mtb-infected macrophages, which is dependent on sustained upregulation of anti-inflammatory microRNA-21 (miR-21) by proliferating mycobacteria. The dampening of glycolysis by miR-21 is mediated through targeting of phosphofructokinase muscle (PFK-M) isoform at the committed step of glycolysis, which facilitates bacterial growth by limiting pro-inflammatory mediators, chiefly interleukin-1β (IL-1β). Read More

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January 2020

7-oxo-DHEA enhances impaired M. tuberculosis-specific T cell responses during HIV-TB coinfection.

J Biomed Sci 2020 Jan 6;27(1):20. Epub 2020 Jan 6.

Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Universidad de Buenos Aires, Instituto de Investigaciones Biomédicas en Retrovirus y Sida (INBIRS), Buenos Aires, Argentina.

Background: Mycobacterium tuberculosis (Mtb) is the causative agent of tuberculosis (TB), affecting approximately one third of the world's population. Development of an adequate immune response will determine disease progression or progress to chronic infection. Risk of developing TB among human immunodeficiency virus (HIV)-coinfected patients (HIV-TB) is 20-30 times higher than those without HIV infection, and a synergistic interplay between these two pathogens accelerates the decline in immunological functions. Read More

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January 2020

Caveolin-1 Controls Vesicular TLR2 Expression, p38 Signaling and T Cell Suppression in BCG Infected Murine Monocytic Myeloid-Derived Suppressor Cells.

Front Immunol 2019 3;10:2826. Epub 2019 Dec 3.

Institute for Virology and Immunobiology, University of Würzburg, Würzburg, Germany.

Monocytic myeloid-derived suppressor cells (M-MDSCs) and granulocytic MDSCs (G-MDSCs) have been found to be massively induced in TB patients as well in murine Mtb infection models. However, the interaction of mycobacteria with MDSCs and its role in TB infection is not well studied. Here, we investigated the role of Cav-1 for MDSCs infected with Bacille-Calmette-Guerín (BCG). Read More

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November 2020

Temporal modulation of host aerobic glycolysis determines the outcome of Mycobacterium marinum infection.

Fish Shellfish Immunol 2020 Jan 24;96:78-85. Epub 2019 Nov 24.

MOE/NHC/CAMS Key Laboratory of Medical Molecular Virology, School of Basic Medical Sciences, Shanghai Medical College, Fudan University, Shanghai, China. Electronic address:

Macrophages are the first-line host defense that the invading Mycobacterium tuberculosis (Mtb) encounters. It has been recently reported that host aerobic glycolysis was elevated post the infection by a couple of virulent mycobacterial species. However, whether this metabolic transition is required for host defense against intracellular pathogens and the underlying mechanisms remain to be further investigated. Read More

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January 2020

BCG stimulation promotes dendritic cell proliferation and expression of VDR and CYP27B1 in vitamin D‑deficient mice.

Mol Med Rep 2019 Dec 30;20(6):5265-5271. Epub 2019 Oct 30.

Department of Orthopedics, General Hospital of Northern Theater Command, Shenyang, Liaoning 110015, P.R. China.

Vitamin D deficiency may lead to an increased risk of tuberculosis. In the present study, the effects of Mycobacterium tuberculosis (Mtb) infection on dendritic cells (DCs) derived from vitamin D‑deficient mice or normal control mice were investigated. A vitamin D‑deficient mouse model was established, and bone marrow‑derived DCs (BMDCs) were isolated and treated with GM‑CSF and interleukin (IL)‑4 for 6 days, followed by an additional 24 h of treatment with Bacillus Calmette‑Guérin (BCG). Read More

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December 2019

An evolutionary recent IFN/IL-6/CEBP axis is linked to monocyte expansion and tuberculosis severity in humans.

Elife 2019 10 22;8. Epub 2019 Oct 22.

Laboratório de Imunobiologia, Departmento de Microbiologia, Imunologia e Parasitologia, Universidade Federal de Santa Catarina, Florianópolis, Brazil.

Monocyte counts are increased during human tuberculosis (TB) but it has not been determined whether () directly regulates myeloid commitment. We demonstrated that exposure to directs primary human CD34 cells to differentiate into monocytes/macrophages. In vitro myeloid conversion did not require type I or type II IFN signaling. Read More

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October 2019

Dynamics of trophoblast differentiation in peri-implantation-stage human embryos.

Proc Natl Acad Sci U S A 2019 11 21;116(45):22635-22644. Epub 2019 Oct 21.

Colorado Center for Reproductive Medicine, Lone Tree, CO 80124;

Single-cell RNA sequencing of cells from cultured human blastocysts has enabled us to define the transcriptomic landscape of placental trophoblast (TB) that surrounds the epiblast and associated embryonic tissues during the enigmatic day 8 (D8) to D12 peri-implantation period before the villous placenta forms. We analyzed the transcriptomes of 3 early placental cell types, cytoTB (CTB), syncytioTB (STB), and migratoryTB (MTB), picked manually from cultured embryos dissociated with trypsin and were able to follow sublineages that emerged from proliferating CTB at the periphery of the conceptus. A unique form of CTB with some features of STB was detectable at D8, while mature STB was at its zenith at D10. Read More

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November 2019