25 results match your criteria mpo-dna complex

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Elevated Myeloperoxidase-DNA Complex Levels in Sera of Patients with IgA Vasculitis.

Pathobiology 2021 Nov 23:1-6. Epub 2021 Nov 23.

Department of Dermatology, St. Marianna University School of Medicine, Kawasaki, Japan.

Introduction: IgA vasculitis is a systemic disease that results from the entrapment of circulating IgA-containing immune complexes in small-vessel walls in the skin, kidneys, and gastrointestinal tract. An excessive formation of neutrophil extracellular traps (NETs) is involved in the pathogenesis of vasculitis, especially in antineutrophil cytoplasmic antibody (ANCA)-associated vasculitis. This study aimed to clarify whether NETs are implicated in IgA vasculitis. Read More

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November 2021

The Quantification of Extracellular Trap Cell Death-Derived Products as Diagnostic Biomarkers for Otitis Media with Antineutrophil Cytoplasmic Antibody-Associated Vasculitis and Eosinophilic Otitis Media.

Otol Neurotol 2021 Nov 19. Epub 2021 Nov 19.

Department of Otolaryngology - Head and Neck Surgery, Faculty of Medicine and Graduate School of Medicine, Hokkaido University, Sapporo, Japan.

Objective: This study aimed to quantify the cell-free deoxyribonucleic acid (DNA), citrullinated-histone H3 (cit-H3)-DNA complex, and myeloperoxidase (MPO)-DNA complex as extracellular trap cell death (ETosis)-derived products in the middle ear fluid, and to identify diagnostic biomarkers for the discrimination of antineutrophil cytoplasmic antibody (ANCA)-associated vasculitis (OMAAV) from eosinophilic otitis media (EOM).

Study Design: Prospective study.

Setting: Tertiary referral center. Read More

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November 2021

Neutrophil extracellular traps in patients with liver cirrhosis and hepatocellular carcinoma.

Sci Rep 2021 09 9;11(1):18025. Epub 2021 Sep 9.

Department of Medicine, Karolinska Institutet, Huddinge, Stockholm, Sweden.

Neutrophil extracellular traps (NETs) are web-like structures consisting of DNA, histones and granule proteins, released from neutrophils in thrombus formation, inflammation, and cancer. We asked if plasma levels of the NET markers myeloperoxidase (MPO)-DNA and citrullinated histone H3 (H3Cit)-DNA, are elevated in liver cirrhosis and hepatocellular carcinoma (HCC) and if the levels correlate with clinical parameters. MPO-DNA, H3Cit-DNA, and thrombin-antithrombin (TAT) complex, as a marker of coagulation activity, were measured using ELISA in plasma from 82 patients with HCC, 95 patients with cirrhosis and 50 healthy controls. Read More

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September 2021

Circulating Myeloperoxidase (MPO)-DNA complexes as marker for Neutrophil Extracellular Traps (NETs) levels and the association with cardiovascular risk factors in the general population.

PLoS One 2021 11;16(8):e0253698. Epub 2021 Aug 11.

Department of Hematology, Erasmus University Medical Center, Rotterdam, The Netherlands.

Introduction: Neutrophil extracellular traps (NETs) are DNA scaffolds enriched with antimicrobial proteins. NETs have been implicated in the development of various diseases, such as cardiovascular disease. Here, we investigate the association of demographic and cardiovascular (CVD) risk factors with NETs in the general population. Read More

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Senkyunolide I protect against lung injury via inhibiting formation of neutrophil extracellular trap in a murine model of cecal ligation and puncture.

Int Immunopharmacol 2021 Oct 2;99:107922. Epub 2021 Jul 2.

Faculty of Anesthesiology, Changhai Hospital, Naval Medical University, Shanghai 200433, China. Electronic address:

Background: Senkyunolide I (SEI), a component of a Chinese herb named Ligusticum Chuanxiong hort, which is included in the formulation of Xuebijing Injection, a medication used to treat sepsis in China. Our previous study showed that SEI was protective against sepsis-associated encephalopathy and the present study was performed to investigate the role of SEI in sepsis-induced lung injury in a murine model of cecal ligation and puncture (CLP).

Methods: SEI (36 mg/kg in 200 μl) or vehicle was administered immediately after CLP surgery. Read More

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October 2021

Common and Rare Variants Genetic Association Analysis of Circulating Neutrophil Extracellular Traps.

Front Immunol 2021 24;12:615527. Epub 2021 Feb 24.

Department of Epidemiology, Erasmus University Medical Center, Rotterdam, Netherlands.

Introduction: Neutrophils contribute to host defense through different mechanisms, including the formation of neutrophil extracellular traps (NETs). The genetic background and underlying mechanisms contributing to NET formation remain unclear.

Materials And Methods: We performed a genome-wide association study (GWAS) and exome-sequencing analysis to identify common and rare genetic variants associated with plasma myeloperoxidase (MPO)-DNA complex levels, a biomarker for NETs, in the population-based Rotterdam Study cohort. Read More

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Neutrophil extracellular traps exacerbate coagulation and endothelial damage in patients with essential hypertension and hyperhomocysteinemia.

Thromb Res 2021 01 1;197:36-43. Epub 2020 Nov 1.

Department of Cardiology, First Affiliated Hospital of Harbin Medical University, Harbin, 150001, Heilongjiang Province, China. Electronic address:

Patients with essential hypertension (EH) and hyperhomocysteinemia (HHCY) suffer from more increased thrombotic events than those in EH alone. However, the underlying mechanisms for this effect are not well understood. This study hypothesized that neutrophil extracellular trap (NET) releasing may be triggered by HHCY in patients in EH, thereby predisposing them to a more hypercoagulable state. Read More

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January 2021

Ticagrelor Exerts Immune-Modulatory Effect by Attenuating Neutrophil Extracellular Traps.

Int J Mol Sci 2020 May 21;21(10). Epub 2020 May 21.

Laboratory of Molecular Hematology, Department of Medicine, Democritus University of Thrace, 68100 Alexandroupolis, Greece.

Neutrophils through the release of neutrophil extracellular traps (NETs) containing active tissue factor (TF) are key components of thrombo-inflammation. Platelets-neutrophils interplay in ST elevation myocardial infarction (STEMI) promotes NET formation via inorganic polyphosphates (polyP) released by thrombin-activated platelets. NETs, however, are also induced by biomaterials in a platelet-independent manner. Read More

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Complement Activation in Association with Markers of Neutrophil Extracellular Traps and Acute Myocardial Infarction in Stable Coronary Artery Disease.

Mediators Inflamm 2020 26;2020:5080743. Epub 2020 Mar 26.

Center for Clinical Heart Research, Department of Cardiology, Oslo University Hospital Ullevål, Norway.

Complement activation and neutrophil extracellular traps (NETs) have both been suggested to drive atherosclerotic plaque progression. Although experimental studies suggest interplay between these two innate immunity components, the relevance in patients with coronary artery disease (CAD) is unclear. The aim of this study was to assess associations between complement activation and NETs in patients with stable CAD and examine the role of complement activation on clinical outcome. Read More

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February 2021

Three Ingredients of Safflower Alleviate Acute Lung Injury and Inhibit NET Release Induced by Lipopolysaccharide.

Mediators Inflamm 2020 29;2020:2720369. Epub 2020 Feb 29.

Faculty of Anesthesiology, Changhai Hospital, Naval Medical University, 168 Changhai Road, Shanghai 200433, China.

Xuebijing injection is a Chinese herb compound to treat sepsis in China, but it contains many different kinds of components, and each component may have different effects in treating sepsis. The present study was performed to investigate the effect of three ingredients of Xuebijing, safflor yellow A (SYA), hydroxysafflor yellow A (HSYA), and anhydrosafflor yellow B (AHSYB), in lipopolysaccharide- (LPS-) induced acute lung injury (ALI). LPS (10 mg/kg) was injected intratracheally to induce acute lung injury in mice, which were then treated with SYA, HSYA, and AHSYB. Read More

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January 2021

Neutrophil Extracellular Traps Effectively Control Acute Chikungunya Virus Infection.

Front Immunol 2019 31;10:3108. Epub 2020 Jan 31.

Department of Pharmacology, School of Medicine of Ribeirao Preto, University of São Paulo, Ribeirao Preto, Brazil.

The Chikungunya virus (CHIKV) is a re-emerging arbovirus, in which its infection causes a febrile illness also commonly associated with severe joint pain and myalgia. Although the immune response to CHIKV has been studied, a better understanding of the virus-host interaction mechanisms may lead to more effective therapeutic interventions. In this context, neutrophil extracellular traps (NETs) have been described as a key mediator involved in the control of many pathogens, including several bacteria and viruses, but no reports of this important protective mechanism were documented during CHIKV infection. Read More

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November 2020

Neutrophil extracellular traps induced by IL-8 aggravate atherosclerosis via activation NF-κB signaling in macrophages.

Cell Cycle 2019 Nov 8;18(21):2928-2938. Epub 2019 Sep 8.

Department of Cardiovascular, The First Affiliated Hospital of Harbin Medical University , Harbin , Heilongjiang , China.

Here, we sought to explore the underlying role of interleukin (IL)-8 in neutrophil extracellular traps (NETs) formation during atherosclerosis (AS). The concentration of pro-inflammatory cytokines IL-8, IL-6 and IL-1β was determined by enzyme-linked immunosorbent assay (ELISA). NETs formation was evaluated by immunofluorescence and myeloperoxidase (MPO)-DNA complex ELISA. Read More

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November 2019

Neutrophil Extracellular Traps.

Authors:
Balázs Rada

Methods Mol Biol 2019 ;1982:517-528

College of Veterinary Medicine, Department of Infectious Diseases, University of Georgia, Athens, GA, USA.

Neutrophil extracellular traps (NETs) are made of a network of extracellular strings of DNA that bind pathogenic microbes. Histones and several neutrophil granule proteins associated with the DNA framework damage entrapped microorganisms. Reactive oxygen species generated by the neutrophil NADPH oxidase have been shown to be essential to mediate NET release by several stimuli including numerous pathogenic bacteria. Read More

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January 2020

Neutrophil Extracellular Traps May Contribute to the Pathogenesis in Adult-onset Still Disease.

J Rheumatol 2019 12 1;46(12):1560-1569. Epub 2019 May 1.

From the departments of Rheumatology, Pathology, and Microbiology, Ajou University School of Medicine, Suwon, Korea.

Objective: Release of neutrophil extracellular traps (NET) has been described as an effector mechanism of polymorphonuclear neutrophils in several inflammatory diseases. Thus, this study was performed to evaluate the role of NET in the pathogenesis of adult-onset Still disease (AOSD).

Methods: We determined the serum levels of NET molecules and investigated their associations with clinical disease activities in patients with AOSD. Read More

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December 2019

Neutrophil extracellular traps (NETs) are increased in the alveolar spaces of patients with ventilator-associated pneumonia.

Crit Care 2018 12 27;22(1):358. Epub 2018 Dec 27.

Division of Rheumatology, Department of Medicine, University of Washington, 750 Republican Street, Rm. E563, Box 358060, Seattle, WA, 98109, USA.

Background: Neutrophils release neutrophil extracellular traps (NETs) in response to invading pathogens. Although NETs play an important role in host defense against microbial pathogens, they have also been shown to play a contributing mechanistic role in pathologic inflammation in the absence of infection. Although a role for NETs in bacterial pneumonia and acute respiratory distress syndrome (ARDS) is emerging, a comprehensive evaluation of NETs in the alveolar space of critically ill patients has yet to be reported. Read More

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December 2018

The Diagnostic and Clinical Utility of the Myeloperoxidase-DNA Complex as a Biomarker in Otitis Media With Antineutrophil Cytoplasmic Antibody-associated Vasculitis.

Otol Neurotol 2019 02;40(2):e99-e106

Department of Otolaryngology - Head and Neck Surgery.

Objective: This prospective study aimed to evaluate the diagnostic and clinical utility of the myeloperoxidase (MPO)-DNA complex as a NETosis-derived product in the middle ear fluid of patients with otitis media with antineutrophil cytoplasmic antibody-associated vasculitis (OMAAV).

Study Design: Prospective study.

Setting: Tertiary referral center. Read More

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February 2019

Elevated Plasma Levels of Cell-Free DNA During Liver Transplantation Are Associated With Activation of Coagulation.

Liver Transpl 2018 12;24(12):1716-1725

Surgical Research Laboratory, University of Groningen, Groningen, the Netherlands.

Patients undergoing liver transplantation have complex changes in their hemostatic system, and the net effect of these changes appears to be a "rebalanced" hemostatic profile. Recently, a process called NETosis in which a neutrophil expels DNA and proteins that form a weblike structure, has been described as a mechanism of pathogen entrapment. Increasing evidence suggests a pivotal role for neutrophil extracellular traps (NETs) and their main component, cell-free DNA (cfDNA), in activation of coagulation. Read More

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December 2018

Elevated Level of Myeloperoxidase-Deoxyribonucleic Acid Complex in the Middle Ear Fluid Obtained From Patients With Otitis Media Associated With Antineutrophil Cytoplasmic Antibody-associated Vasculitis.

Otol Neurotol 2018 04;39(4):e257-e262

Faculty of Health Sciences.

Objective: The purpose was to explore the presence of myeloperoxidase (MPO)-deoxyribonucleic acid (DNA) complex as a surrogate marker of neutrophil extracellular traps (NETs) in the middle ear fluid, and to clarify the correlation between its quantifiable level and hearing outcome in patients with otitis media associated with antineutrophil cytoplasmic antibody (ANCA)-associated vasculitis (AAV).

Study Design: Prospective study.

Setting: Tertiary referral center. Read More

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High Level of Neutrophil Extracellular Traps Correlates With Poor Prognosis of Severe Influenza A Infection.

J Infect Dis 2018 01;217(3):428-437

Institute of Infectious Diseases, Capital Medical University, China.

Background: Most patients with severe infection with influenza A virus (IAV) progress to acute respiratory distress syndrome and even multiple organ dysfunction syndrome (MODS). Neutrophil extracellular traps (NETs) can be induced by pathogens and are responsible for immune tissue damage. We conducted a prospective study on the production and effects of NETs in H7N9 and H1N1 patients. Read More

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January 2018

IL-33 exacerbates liver sterile inflammation by amplifying neutrophil extracellular trap formation.

J Hepatol 2017 Sep 21. Epub 2017 Sep 21.

Department of Surgery, University of Pittsburgh, Pittsburgh, PA, United States; Department of Surgery, Union Hospital, Huazhong University of Science and Technology, Wuhan, PR China. Electronic address:

Background & Aims: Neutrophils and liver sinusoidal endothelial cells (LSECs) both contribute to sterile inflammatory injury during ischemia/reperfusion (I/R), a well-known liver surgical stress. Interleukin-33 (IL-33) has been shown to drive neutrophil infiltration during inflammatory responses through its receptor ST2. We recently reported that infiltrating neutrophils form neutrophil extracellular traps (NETs), which exacerbate sterile inflammatory injury in liver I/R. Read More

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September 2017

Nicotine drives neutrophil extracellular traps formation and accelerates collagen-induced arthritis.

Rheumatology (Oxford) 2017 04;56(4):644-653

Division of Immunology and Rheumatology, Stanford University School of Medicine, Stanford, CA, USA.

Objectives: The aim was to investigate the effects of nicotine on neutrophil extracellular traps (NETs) formation in current and non-smokers and on a murine model of RA.

Methods: We compared spontaneous and phorbol 12-myristate 13-acetate-induced NETosis between current and non-smokers by DNA release binding. Nicotine-induced NETosis from non-smokers was assessed by DNA release binding, NET-specific (myeloperoxidase (MPO)-DNA complex) ELISA and real-time fluorescence microscopy. Read More

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NETosis markers: Quest for specific, objective, and quantitative markers.

Clin Chim Acta 2016 Aug 31;459:89-93. Epub 2016 May 31.

Faculty of Health Sciences, Hokkaido University, Sapporo, Japan. Electronic address:

More than 10years have passed since the discovery of neutrophil extracellular traps (NETs) in 2004. NETs are extracellular web-like DNA decorated with antimicrobial proteins, which are released from activated neutrophils. The state of neutrophils with NET formation is called NETosis. Read More

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Circulating Neutrophil Extracellular Trap Levels in Well-Controlled Type 2 Diabetes and Pathway Involved in Their Formation Induced by High-Dose Glucose.

Pathobiology 2016 18;83(5):243-51. Epub 2016 May 18.

Division of Rheumatology, Endocrinology and Nephrology, Hokkaido University Graduate School of Medicine, Sapporo, Japan.

Objectives: Although intensive therapy for type 2 diabetes (T2D) prevents microvascular complications, 10% of well-controlled T2D patients develop microangiopathy. Therefore, the identification of risk markers for microvascular complications in well-controlled T2D patients is important. Recent studies have demonstrated that high-dose glucose induces neutrophil extracellular trap (NET) formation, which can be a risk for microvascular disorders. Read More

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January 2017

Association of neutrophil extracellular traps with endometriosis-related chronic inflammation.

Eur J Obstet Gynecol Reprod Biol 2014 Dec 31;183:193-200. Epub 2014 Oct 31.

Institute of Biochemistry, Medical School, Justus-Liebig-University, 35392 Giessen, Germany.

Objective: To study if neutrophil extracellular traps (NETs) are present in the peritoneal fluid of endometriosis patients. NETs play a crucial role in fighting against microorganisms. However, exaggerated NET production may lead to tissue damage in their vicinity in pathological conditions. Read More

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December 2014

Neutrophil extracellular traps regulate IL-1β-mediated inflammation in familial Mediterranean fever.

Ann Rheum Dis 2016 Jan 26;75(1):269-77. Epub 2014 Sep 26.

Laboratory of Molecular Hematology, Democritus University of Thrace, Alexandroupolis, Greece First Department of Internal Medicine, University Hospital of Alexandroupolis, Alexandroupolis, Greece.

Objective: Inflammatory attacks of familial Mediterranean fever (FMF) are characterised by circulation and influx of high number of polymorphonuclear neutrophils (PMN) in the affected sites and profound therapeutic effect of IL-1β inhibitors. We investigated the role of neutrophil extracellular traps (NET) in the pathogenesis of FMF, and their involvement in IL-1β production.

Methods: Blood samples were obtained from six FMF patients during remissions and from three patients during attacks. Read More

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January 2016
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