314,940 results match your criteria motility


Towards increasing stallion sperm longevity by storage at subzero temperatures in the absence of ice.

J Equine Vet Sci 2021 Nov 5;108:103802. Epub 2021 Nov 5.

Unit for Reproductive Medicine, Clinic for Horses, University of Veterinary Medicine Hannover, Hannover, Germany.

The aim of cell preservation technologies is to slow down damaging reactions by lowering the storage temperature. Upon dilution in a stabilizing extender, stallion sperm can be stored at refrigerator temperatures for several days. Cryopreservation allows storage for decades, but freezing and thawing cause damage and viability losses. Read More

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November 2021

Transcriptome analysis of biofilm formation under aerobic and microaerobic conditions in clinical isolates of Campylobacter spp.

Res Vet Sci 2021 Nov 19;142:24-30. Epub 2021 Nov 19.

Department of Microbiology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran; Microbial Biotechnology Research Center, Iran University of Medical Sciences, Tehran, Iran. Electronic address:

It has been well documented that Campylobacter is the leading cause of foodborne infections and bacterial enteritis in high-income countries. The gastrointestinal tract of most warm-blooded animals, such as mammals and poultry, is prone to this pathogen. Infections caused by this bacterium in humans have usually been associated with the consumption of contaminated poultry meat. Read More

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November 2021

The analysis of F-actin structure of mesenchymal stem cells by quantification of fractal dimension.

PLoS One 2021 30;16(11):e0260727. Epub 2021 Nov 30.

Group of Ionic Mechanisms of Cell Signaling, Institute of Cytology of the Russian Academy of Sciences, St-Petersburg, Russia.

The actin cytoskeleton is indispensable for the motility and migration of all types of cells; therefore, it plays a crucial role in the ability of the tissues to repair. Mesenchymal stem cells are intensively used in regenerative medicine, but usually relatively low percent of transplanted cells reaches the injury. To overcome this evident limitation, researchers try to enhance the motility and migration rate of the cells. Read More

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November 2021

Diminished androgen levels are linked to irritable bowel syndrome and cause bowel dysfunction in mice.

J Clin Invest 2021 Nov 30. Epub 2021 Nov 30.

Department of Pediatrics, Boston Children's Hospital, Boston, United States of America.

Functional gastrointestinal disorders (FGIDs) have prominent sex differences in incidence, symptoms, and treatment response that are not well understood. Androgens are steroid hormones present at much higher levels in males than females and could be involved in these differences. In adults with irritable bowel syndrome (IBS), a FGID that affects 5-10% of the population worldwide, we found that free testosterone levels were lower than those in healthy controls and inversely correlated with symptom severity. Read More

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November 2021

Biosensors for the Detection of .

Acc Chem Res 2021 Nov 30. Epub 2021 Nov 30.

National Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China.

Conspectus, present in two forms of vegetative cells and spores, is a pathogen that infects humans through contact with infected animals or contaminated animal products and is also maliciously used in terrorist acts. Therefore, a rapid and sensitive test for is necessary but challenging. The challenge comes from the following aspects: an accurate distinction of from other species due to their high genomic similarity and the horizontal gene transfer between members; direct detection of the spores without damaging them for component extraction to avoid the risk of spore atomization; and the rapid detections of in complex samples, such as soil and suspicious powders, without sample pretreatments and expensive large-scale equipment. Read More

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November 2021

Improvement of Astragalin on Spermatogenesis in Oligoasthenozoospermia Mouse Induced by Cyclophosphamide.

Reprod Sci 2021 Nov 30. Epub 2021 Nov 30.

Department of Obstetrics and Gynecology, Key Laboratory for Gynecologic Oncology Gansu Province, The First Hospital of Lanzhou University, Lanzhou, China.

More than 40% of infertile men are diagnosed with oligoasthenozoospermia and the incidence is still rising, but the effective treatments are not been found until now. Astragalin, one of the main active ingredients in traditional Chinese medicine, may be effective in the treatment of oligoasthenozoospermia. This study investigated the pharmacological effects of astragalin for treatment of oligoasthenozoospermia in male mice, induced by cyclophosphamide (CTX). Read More

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November 2021

A novel live-cell imaging assay reveals regulation of endosome maturation.

Elife 2021 Nov 30;10. Epub 2021 Nov 30.

Biozentrum, University of Basel, Basel, Switzerland.

Cell-cell communication is an essential process in life, with endosomes acting as key organelles for regulating uptake and secretion of signaling molecules. Endocytosed material is accepted by the sorting endosome where it either is sorted for recycling or remains in the endosome as it matures to be degraded in the lysosome. Investigation of the endosome maturation process has been hampered by the small size and rapid movement of endosomes in most cellular systems. Read More

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November 2021

Diurnal changes of colonic motility and regulatory factors for colonic motility in Suncus murinus.

Neurogastroenterol Motil 2021 Nov 30:e14302. Epub 2021 Nov 30.

Division of Life Science, Graduate School of Science and Engineering, Saitama University, Saitama, Japan.

Background: The aim of this study was to investigate the fundamental mechanisms of colonic motility in the house musk suncus (Suncus murinus) as an established animal model of gut motility.

Methods: To measure gut motility in free-moving conscious suncus, strain gauge force transducers were implanted on the serosa of the colon and gastric body.

Key Results: We recorded diurnal changes in colonic motility and observed the relationship between feeding and colonic motility. Read More

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November 2021

Hyperpolarization-activated cyclic nucleotide-gated channels working as pacemaker channels in colonic interstitial cells of Cajal.

J Cell Mol Med 2021 Nov 29. Epub 2021 Nov 29.

Department of Physiology, College of Medicine, Chosun University, Gwangju, Korea.

Hyperpolarization-activated cyclic nucleotide-gated (HCN) channels function as pacemaker channels in spontaneously active cells. We studied the existence of HCN channels and their functional roles in the interstitial cells of Cajal (ICC) from the mouse colon using electrophysiological, immunohistochemical and molecular techniques. HCN1 and HCN3 channels were detected in anoctamin-1 (Ca -activated Cl channel; ANO1)-positive cells within the muscular and myenteric layers in colonic tissues. Read More

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November 2021

In vitro spermicidal effect of Thevetia Peruviana leaves on human spermatozoa.

Andrologia 2021 Nov 29:e14323. Epub 2021 Nov 29.

Department of Biomedical Laboratory Science and Management, Vidyasagar University, Midnapore, India.

An in vitro spermicidal effect of aqua-methanolic (2:3) extract of Thevetia peruviana leaves on human spermatozoa was evaluated in a dose-dependent manner (20, 40, 80 and 160 mg/ml) at a 1:1 ratio. Sperm motility, viability, hypo-osmotic swelling (HOS) and acrosomal status and function tests were performed immediately (20 s), and after 5 and 10 min of exposure of the spermatozoa to the extract of Thevetia peruviana leaves at different dose concentrations. Nuclear chromatin decondensation (NCD) test, DNA fragmentation test and sperm revival test were also evaluated. Read More

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November 2021

Protective effect of saponin on sperm DNA fragmentation of mice treated with cyclophosphamide.

Andrologia 2021 Nov 29:e14336. Epub 2021 Nov 29.

Research Laboratory for Embryology and Stem Cells, Department of Anatomical Sciences, School of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran.

Cyclophosphamide (CP) is a common chemotherapy drug with the testicular damage. The aim of this study was to investigate the effect of saponin (SP) on the toxicity of CP in the male reproductive system. Following an experimental pilot study for determining SP dose, 40 male mice (32 ± 3 g) were divided into five groups (n = 8): control, sham (normal saline 0. Read More

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November 2021

Isolation and Transfection of Citrus Protoplasts with Citrus Exocortis Viroid.

Methods Mol Biol 2022 ;2316:39-54

Department of Microbiology and Plant Pathology, University of California Riverside, Riverside, CA, USA.

Viroids are RNA-based infectious agents that are single-stranded, covalently closed circular, non-coding, and naked. Unlike RNA viruses, which at least encode proteins for replication, encapsidation, and movement, lack of protein-coding capacity of viroids makes them completely reliant on host for replication and movement. The high genetic diversity in viroids is believed to be due to the absence of proof-reading activity of the host RNA polymerases, the large population size, and the rapid rate of replication. Read More

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January 2022

Differential Regulation of Neurite Outgrowth and Growth Cone Morphology by 3D Fibronectin and Fibronectin-Collagen Extracellular Matrices.

Mol Neurobiol 2021 Nov 30. Epub 2021 Nov 30.

Department of Molecular Biology, Princeton University, Princeton, NJ, 08544, USA.

The extracellular matrix (ECM) plays a critical role in development, homeostasis, and regeneration of tissue structures and functions. Cell interactions with the ECM are dynamic and cells respond to ECM remodeling by changes in morphology and motility. During nerve regeneration, the ECM facilitates neurite outgrowth and guides axons with target specificity. Read More

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November 2021

Epitope-coated polymer particles elicit neutralising antibodies against Plasmodium falciparum sporozoites.

NPJ Vaccines 2021 Nov 29;6(1):141. Epub 2021 Nov 29.

Centre for Cell Factories and Biopolymers, Griffith Institute for Drug Discovery, Griffith University, Brisbane, QLD, Australia.

The current Malaria RTS,S vaccine is based on virus-like particles (VLPs) comprising the NANP repetitive epitopes from the cicumsporozoite protein (CSP) of Plasmodium falciparum. This vaccine has limited efficacy, only preventing severe disease in about 30% of vaccinated individuals. A more efficacious vaccine is urgently needed to combat malaria. Read More

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November 2021

Moxibustion Regulates Gastrointestinal Motility via HCN1 in Functional Dyspepsia Rats.

Med Sci Monit 2021 Nov 30;27:e932885. Epub 2021 Nov 30.

School of Nursing, Second Military Medical University, Shanghai, China (mainland).

BACKGROUND Moxibustion therapy has been found to ameliorate clinical symptoms of functional dyspepsia (FD). We aimed to examine the regulatory effect of moxibustion on the gastrointestinal (GI) motility in FD and explore the underlying mechanism based on the hyperpolarization-activated cyclic nucleotide-gated cation channel 1 (HCN1). MATERIAL AND METHODS Moxibustion therapy was used in FD rats induced by using classic tail-pinch and irregular feeding. Read More

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November 2021

Analysis of changes in microbiome compositions related to the prognosis of colorectal cancer patients based on tissue-derived 16S rRNA sequences.

J Transl Med 2021 Nov 29;19(1):485. Epub 2021 Nov 29.

Division of Biomedical Convergence, College of Biomedical Science, Institute of Bioscience & Biotechnology, Kangwon National University, Chuncheon, 24341, Republic of Korea.

Background: Comparing the microbiome compositions obtained under different physiological conditions has frequently been attempted in recent years to understand the functional influence of microbiomes in the occurrence of various human diseases.

Methods: In the present work, we analyzed 102 microbiome datasets containing tumor- and normal tissue-derived microbiomes obtained from a total of 51 Korean colorectal cancer (CRC) patients using 16S rRNA amplicon sequencing. Two types of comparisons were used: 'normal versus (vs. Read More

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November 2021

Mixed sexually transmitted infections in infertile couples: empirical treatment and influence on semen quality.

Recent Adv Antiinfect Drug Discov 2021 Nov 28. Epub 2021 Nov 28.

Department of Urology, Danylo Halytsky National Medical University, Lviv. Ukraine.

Background: Trichomonas vaginalis (TV), Mycoplasma genitalium (MG) and Ureaplasma urealyticum (UU) are common sexually transmitted infections (STIs) that are diagnosed in infertile couples (cps). In cases with their simultaneous presence in a patient (mixed STI, MSTIs), treatment is complicated by the different sensitivity of microorganisms to antibacterial drugs. Moreover, in cases of complicated infections, the empirical treatment should be started before obtaining the results of drug susceptibilities. Read More

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November 2021

Use Low Ozone Dosages has Positive Effects on the Cooling and Cryopreservation of Equine Semen.

J Equine Vet Sci 2021 Oct 29;108:103800. Epub 2021 Oct 29.

Veterinary Medicine and Animal Science of the Federal University of Mato Grosso do Sul Foundation - FAMEZ/UFMS, Campo Grande, Mato Grosso do Sul, Brazil. Electronic address:

The aim of this study was to determine an ozone dosage capable of inducing pro-oxidation, and to verify its action on sperm cells during the process of cooling and cryopreservation of equine semen. In this study, we evaluated the ozone concentrations of 2µg/mL,15µg/mL, 30µg/mL e 60 µg/mL added in equine semen cooling and freezing extenders. Samples were evaluated for sperm kinetics patterns, function of sperm structures and lipid peroxidation. Read More

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October 2021

Epididymal mRNA and miRNA transcriptome analyses reveal important genes and miRNAs related to sperm motility in roosters.

Poult Sci 2021 Oct 21;101(1):101558. Epub 2021 Oct 21.

Animal Science and Technology College, Beijing University of Agriculture, Beijing 102206, China. Electronic address:

Sperm motility is a crucial trait in chicken production, and the epididymis is an essential organ in the reproductive system. Currently, the molecular mechanisms underlying sperm motility in the epididymis are unclear. In this study, 8 cDNA libraries and eight miRNA libraries were constructed from roosters (4 chickens per group) with diverse sperm motility. Read More

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October 2021

DILUENT CONTAINING DIMETHYLFORMAMIDE ADDED WITH SUCROSE IMPROVES IN VITRO QUALITY AFTER FREEZING/THAWING STALLION SPERM.

J Equine Vet Sci 2021 Nov 26:103825. Epub 2021 Nov 26.

Andrology Laboratory (ANDROLAB), Department of Veterinary Medicine, Universidade Federal Rural de Pernambuco, 52171-900, Dois Irmãos, Recife, Pernambuco, Brazil.

The aim of this study was to investigate the effects of sucrose on post-thawed equine semen quality. Semen samples (n = 24) were collected from six stallions. They were diluted (200 × 10 sperm/ml) in a freezing medium based on skimmed milk, egg yolk, dimethylformamide, and supplemented with sucrose at concentrations of 0 (Control), 25, 50, and 100 mM and in a commercial extender (BotuCrio®). Read More

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November 2021

Motor domain-mediated autoinhibition dictates axonal transport by the kinesin UNC-104/KIF1A.

PLoS Genet 2021 Nov 29;17(11):e1009940. Epub 2021 Nov 29.

College of Life Sciences, University of Chinese Academy of Sciences, Beijing, China.

The UNC-104/KIF1A motor is crucial for axonal transport of synaptic vesicles, but how the UNC-104/KIF1A motor is activated in vivo is not fully understood. Here, we identified point mutations located in the motor domain or the inhibitory CC1 domain, which resulted in gain-of-function alleles of unc-104 that exhibit hyperactive axonal transport and abnormal accumulation of synaptic vesicles. In contrast to the cell body localization of wild type motor, the mutant motors accumulate on neuronal processes. Read More

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November 2021

Protein domain-based prediction of drug/compound-target interactions and experimental validation on LIM kinases.

PLoS Comput Biol 2021 Nov 29;17(11):e1009171. Epub 2021 Nov 29.

CanSyL, Graduate School of Informatics, Middle East Technical University, Ankara, Turkey.

Predictive approaches such as virtual screening have been used in drug discovery with the objective of reducing developmental time and costs. Current machine learning and network-based approaches have issues related to generalization, usability, or model interpretability, especially due to the complexity of target proteins' structure/function, and bias in system training datasets. Here, we propose a new method "DRUIDom" (DRUg Interacting Domain prediction) to identify bio-interactions between drug candidate compounds and targets by utilizing the domain modularity of proteins, to overcome problems associated with current approaches. Read More

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November 2021

Diacylglycerol kinase A is essential for polymyxin resistance provided by EptA, MCR-1 and other lipid A phosphoethanolamine transferases.

J Bacteriol 2021 Nov 29:JB0049821. Epub 2021 Nov 29.

Department of Infectious Diseases, College of Veterinary Medicine, University of Georgia, Athens GA, 30602.

Gram-negative bacteria utilize glycerophospholipids (GPLs) as phospho-form donors to modify various surface structures. These modifications play important roles in bacterial fitness in diverse environments influencing cell motility, recognition by the host during infection, and antimicrobial resistance. A well-known example is the modification of the lipid A component of lipopolysaccharide by the phosphoethanolamine (pEtN) transferase EptA that utilizes phosphatidyethanoalmine (PE) as the phospho-form donor. Read More

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November 2021

Characterization of Edwardsiella piscicida CK108 flagellin genes and evaluation of their potential as vaccine targets in the zebrafish model.

J Fish Dis 2021 Nov 29. Epub 2021 Nov 29.

Department of Microbiology, Pusan National University, Busan, Korea.

The control of bacterial pathogens, including Edwardsiella piscicida, in the aquaculture industry has high economic importance. This study aimed to identify a potential live vaccine candidate against E. piscicida infection to minimize the side effects and elicit immunity in the host. Read More

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November 2021

Achievements in bacterial flagellar research with focus on Vibrio species.

Microbiol Immunol 2021 Nov 29. Epub 2021 Nov 29.

Division of Biological Science, Graduate School of Science, Nagoya University.

In 1980's, the most genes involved in the bacterial flagellar function and formation had been isolated though many of their functions or roles were not clarified. Bacterial flagella are the primary locomotive organ and are not necessary for growing in vitro but are probably essential for living in natural condition and are involved in the pathogenicity. In vitro, the flagella-deficient strains can grow at rates similar to wild-type strains. Read More

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November 2021

Estrogen receptor variant ERα46 and insulin receptor drive in primary breast cancer cells growth effects and interleukin 11 induction prompting the motility of cancer-associated fibroblasts.

Clin Transl Med 2021 11;11(11):e516

Department of Pharmacy, Health and Nutritional Sciences, University of Calabria, Rende, Italy.

Among the prognostic and predictive biomarkers of breast cancer (BC), the role of estrogen receptor (ER)α wild-type has been acknowledged, although the action of certain ERα splice variants has not been elucidated. Insulin/insulin receptor (IR) axis has also been involved in the progression and metastasis of BC. For instance, hyperinsulinemia, which is often associated with obesity and type 2 diabetes, may be a risk factor for BC. Read More

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November 2021

Phylogenetic analysis of the bacterial Pro-Pro-endopeptidase domain reveals a diverse family including secreted and membrane anchored proteins.

Curr Res Microb Sci 2021 Dec 26;2:100024. Epub 2021 Feb 26.

Center for Proteomics and Metabolomics, Leiden University Medical Center, Albinusdreef 2, PO Box 9600, 2300 RC Leiden, the Netherlands.

Pro-Pro-endopeptidases (PPEP, EC 3.4.24. Read More

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December 2021

Auditory stimulation during sleep suppresses spike activity in benign epilepsy with centrotemporal spikes.

Cell Rep Med 2021 Nov 26;2(11):100432. Epub 2021 Oct 26.

Institute of Medical Psychology and Behavioral Neurobiology, University of Tübingen, 72076 Tübingen, Germany.

Benign epilepsy with centrotemporal spikes (BECTS) is a common form of childhood epilepsy linked to diverse cognitive abnormalities. The electroencephalogram of patients shows focal interictal epileptic spikes, particularly during non-rapid eye movement (NonREM) sleep. Spike formation involves thalamocortical networks, which also contribute to the generation of sleep slow oscillations (SOs) and spindles. Read More

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November 2021

FoxM1 insufficiency hyperactivates Ect2-RhoA-mDia1 signaling to drive cancer.

Nat Cancer 2020 Oct 12;1(10):1010-1024. Epub 2020 Oct 12.

Department of Biochemistry and Molecular Biology, Mayo Clinic Rochester, MN, USA.

FoxM1 activates genes that regulate S-G2-M cell-cycle progression and, when overexpressed, is associated with poor clinical outcome in multiple cancers. Here we identify FoxM1 as a tumor suppressor in mice that, through its N-terminal domain, binds to and inhibits Ect2 to limit the activity of RhoA GTPase and its effector mDia1, a catalyst of cortical actin nucleation. FoxM1 insufficiency impedes centrosome movement through excessive cortical actin polymerization, thereby causing the formation of non-perpendicular mitotic spindles that missegregate chromosomes and drive tumorigenesis in mice. Read More

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October 2020

A 150 kDa Protein Derived from Bull Seminal Plasma Extended the Survival Time of Kacang Goat Sperm Stored at 5°C.

Vet Med Int 2021 18;2021:1470209. Epub 2021 Nov 18.

Division of Veterinary Reproduction, Faculty of Veterinary Medicine, Universitas Airlangga, Kampus C Unair, Mulyorejo, Surabaya 60115, Indonesia.

Artificial insemination has proven to be an effective method for increasing population size and genetic quality of Kacang goats. However, innovation is required to maintain the quality of Kacang goat semen in storage. This study aimed to examine the effects of supplementing the 150 kDa protein assumed as IGF-I complex derived from bull seminal plasma in skim milk-egg yolk extender on the quality of Kacang goat sperm stored at 5°C. Read More

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November 2021