5,206 results match your criteria monopolar


Gene transfer by electroporation with high frequency bipolar pulses in vitro.

Bioelectrochemistry 2021 Mar 22;140:107803. Epub 2021 Mar 22.

University of Ljubljana, Faculty of Electrical Engineering, Tržaška 25, 1000 Ljubljana, Slovenia. Electronic address:

High-frequency bipolar pulses (HF-BP) have been demonstrated to be efficient for membrane permeabilization and irreversible electroporation. Since membrane permeabilization has been achieved using HF-BP pulses we hypothesized that with these pulses we can also achieve successful gene electrotransfer (GET). Three variations of bursts of 2 µs bipolar pulses with 2 µs interphase delay were applied in HF-BP protocols. Read More

View Article and Full-Text PDF

Monopolar gene electrotransfer enhances plasmid DNA delivery to skin.

Bioelectrochemistry 2021 Apr 17;140:107814. Epub 2021 Apr 17.

Department of Medical Engineering, University of South Florida, Tampa, FL, United States.

A novel monopolar electroporation system and methodologies were developed for in vivo electroporation intended for potential clinical applications such as gene therapy. We hypothesized that an asymmetric anode/cathode electrode applicator geometry could produce favorable electric fields for electroporation, without the typical drawback associated with traditional needle and parallel plate geometries. Three monopolar electrode applicator prototypes were built and tested for gene delivery of reporter genes to the skin in a guinea pig model. Read More

View Article and Full-Text PDF

The Putative RNA-Binding Protein Dri1 Promotes the Loading of Kinesin-14/Klp2 to the Mitotic Spindle and Is Sequestered into Heat-Induced Protein Aggregates in Fission Yeast.

Int J Mol Sci 2021 Apr 30;22(9). Epub 2021 Apr 30.

Laboratory of Molecular and Chemical Cell Biology, Graduate School of Integrated Sciences for Life, Hiroshima University, 1-3-1 Kagamiyama, Higashi-Hiroshima, Hiroshima 739-8530, Japan.

Cells form a bipolar spindle during mitosis to ensure accurate chromosome segregation. Proper spindle architecture is established by a set of kinesin motors and microtubule-associated proteins. In most eukaryotes, kinesin-5 motors are essential for this process, and genetic or chemical inhibition of their activity leads to the emergence of monopolar spindles and cell death. Read More

View Article and Full-Text PDF

X-ray Crystal Structure-Guided Design and Optimization of 7-Pyrrolo[2,3-]pyrimidine-5-carbonitrile Scaffold as a Potent and Orally Active Monopolar Spindle 1 Inhibitor.

J Med Chem 2021 May 4. Epub 2021 May 4.

College of Pharmacy and Yonsei Institute of Pharmaceutical Sciences, Yonsei University, 85 Songdogwahak-ro, Yeonsu-gu, Incheon 21983, South Korea.

Triple-negative breast cancer (TNBC) is an aggressive breast-cancer subtype associated with poor prognosis and high relapse rates. Monopolar spindle 1 kinase (MPS1) is an apical dual-specificity protein kinase that is over-expressed in TNBC. We herein report a highly selective MPS1 inhibitor based on a 7-pyrrolo[2,3-]pyrimidine-5-carbonitrile scaffold. Read More

View Article and Full-Text PDF

Proximity resonances of water-entrained air bubbles near acoustically reflecting boundaries.

J Acoust Soc Am 2021 Apr;149(4):2477

Institute of Physics, Pontificia Universidad Católica de Chile, Santiago, Región Metropolitana, Chile.

The acoustic resonances of radiatively damped air bubbles in water near reflecting boundaries are investigated by representing the bubble and its image by two bubbles in a full space, ensonified by two incident fields. Results obtained using an analytic monopole theory are compared with those of a coupled spherical harmonic technique and a boundary element method. Near a rigid boundary, the resonance frequency is reduced, and the response characteristics are determined by the predominant monopolar character of the individual bubble motion, with small changes in peak amplitude and Q. Read More

View Article and Full-Text PDF

ACG Clinical Guideline: Upper Gastrointestinal and Ulcer Bleeding.

Am J Gastroenterol 2021 May;116(5):899-917

Division of Gastroenterology and Farncombe Family Digestive Health Research Institute, Department of Medicine, McMaster University, Hamilton, Ontario, Canada.

We performed systematic reviews addressing predefined clinical questions to develop recommendations with the GRADE approach regarding management of patients with overt upper gastrointestinal bleeding. We suggest risk assessment in the emergency department to identify very-low-risk patients (e.g. Read More

View Article and Full-Text PDF

Treatment of Patellofemoral Pain Syndrome with Dielectric Radiofrequency Diathermy: A Preliminary Single-Group Study with Six-Month Follow-Up.

Medicina (Kaunas) 2021 Apr 28;57(5). Epub 2021 Apr 28.

Department of Medical-Surgical Therapy, Medicine Faculty, University of Extremadura, 06071 Badajoz, Spain.

: Notwithstanding patellofemoral pain syndrome (PFPS) being one of the most common causes of pain in the front of the knee in outpatients, few studies have shown the effects of radiofrequency on knee pain and function in this population. The aim of the present study was to determine whether outpatients diagnosed with PFPS obtained improvement in pain and function after treatment by dynamic application of monopolar dielectric diathermy by emission of radiofrequency (MDR). : An experimental study was conducted with 27 subjects with PFPS. Read More

View Article and Full-Text PDF

Minimally invasive laparoscopic technique for lymph node dissection in penile cancer: the Pelvic and Inguinal Single-site Approach: PISA technique.

Urology 2021 Apr 26. Epub 2021 Apr 26.

Servicio de Urología. Hospital General Universitario Gregorio Marañón, Madrid, Spain. Electronic address:

Background: Lymph node dissection(LND) remains the gold standard in the staging and treatment of locally advanced penile cancer(PC).

Objective: To describe our initial experience with a new minimally invasive inguinal and pelvic single-access laparoscopic approach,for performing LND in PC, first described in Urology by our group in 2015: the Pelvic and Inguinal Single Access(PISA) technique (Figure 1).

Material: Between 2015-2018, 10 consecutive patients with different PC stages and indication of inguinal LND (cN0 and ≥pT1G3 or cN1/cN2) were operated by means of the PISA technique (Table 1). Read More

View Article and Full-Text PDF

Removal of Electrocardiogram Artifacts From Local Field Potentials Recorded by Sensing-Enabled Neurostimulator.

Front Neurosci 2021 12;15:637274. Epub 2021 Apr 12.

National Engineering Laboratory for Neuromodulation, Tsinghua University, Beijing, China.

Sensing-enabled neurostimulators are an advanced technology for chronic observation of brain activities, and show great potential for closed-loop neuromodulation and as implantable brain-computer interfaces. However, local field potentials (LFPs) recorded by sensing-enabled neurostimulators can be contaminated by electrocardiogram (ECG) signals due to complex recording conditions and limited common-mode-rejection-ratio (CMRR). In this study, we propose a solution for removing such ECG artifacts from local field potentials (LFPs) recorded by a sensing-enabled neurostimulator. Read More

View Article and Full-Text PDF

Very-Low Energy Monopolar Reduces Post-Tonsillectomy Hemorrhage Versus Standard Energy Techniques.

Laryngoscope 2021 Apr 27. Epub 2021 Apr 27.

Dr Bing Zhang Department of Statistics, University of Kentucky, Lexington, Kentucky, U.S.A.

Objectives/hypothesis: To compare rates of post-tonsillectomy hemorrhage (PTH) between a very-low energy transfer monopolar technique (VLET) and standard energy techniques.

Study Design: Retrospective controlled cohort study.

Methods: All tonsillectomies performed by practice physicians during the period January 1, 2010 to August 31, 2019 were identified. Read More

View Article and Full-Text PDF

Removal of Perirolandic Cavernoma with Direct Cortical Stimulation and Neuronavigation with DTI.

Neurol India 2021 Mar-Apr;69(2):304-306

Deprartment of Neuroanaesthesia, National Institute of Mental Health and Neurosciences, Bangalore, Karnataka, India.

Background And Introduction: Intraoperative neuromonitoring for eloquent cortical lesions is irreplaceable in present-day neurosurgery.

Objective: We report a novel technique of combining awake craniotomy with direct cortical stimulation of the motor cortex using the NIM nerve monitoring system.

Technique: A 17-year-old boy presenting with focal motor seizures, diagnosed with left perirolandinc cavernoma, underwent awake craniotomy and complete excision under direct cortical stimulation with NIM monopolar stimulator electrode to locate the motor cortex. Read More

View Article and Full-Text PDF

The PLASMA System for Transurethral Resection of the Prostate: A NICE Medical Technologies Guidance Update.

Appl Health Econ Health Policy 2021 Apr 26. Epub 2021 Apr 26.

Cedar, Cardiff & Vale University Health Board, Cardiff, UK.

Lower urinary tract symptoms (LUTS) in men commonly occur as a consequence of benign prostatic hyperplasia (BPH), also known as prostate enlargement. Treatments for this can involve electrosurgical removal of a section of the prostate via transurethral resection of the prostate (TURP). This can be performed using either monopolar or bipolar electrosurgery. Read More

View Article and Full-Text PDF

Prospective Clinical Trial of the Latest Generation of Noninvasive Monopolar Radiofrequency for the Treatment of Facial and Upper Neck Skin Laxity.

Dermatol Surg 2021 Apr 16. Epub 2021 Apr 16.

Cosmetic Laser Dermatology, San Diego, California; Slocum-Dickson Medical Group, New Hartford, New York; Westlake Dermatology, Dallas, Texas; Department of Dermatology, Mayo Clinic, Phoenix, Arizona.

Abstract: Aging of the face and upper neck is a complex process characterized by loss of collagen and elastic fibers, resulting in clinical skin laxity. Noninvasive interventions such as monopolar capacitively coupled radiofrequency (MRF) have gained popularity, offering a safer and more convenient alternative to traditional surgical face lift. The latest MRF device features larger tips, vibration, cooling, and impedance matching. Read More

View Article and Full-Text PDF

Switching monopolar radiofrequency ablation improves long-term outcomes of medium-sized hepatocellular carcinoma.

Eur Radiol 2021 Apr 25. Epub 2021 Apr 25.

Division of Hepatology, Liver Research Unit, Department of Gastroenterology and Hepatology, Linkou Chang Gung Memorial Hospital, Chang Gung University, 5, Fu-Hsin St., Kwei-Shan, Taoyuan, Taiwan, 333.

Objectives: Radiofrequency ablation (RFA) of medium-sized (3-5 cm) hepatocellular carcinoma (HCC) is suboptimal. Switching monopolar RFA (SW-RFA) enlarges the ablative volume to better cover larger tumors. This study aims to compare the long-term outcomes of medium-sized HCC treated by either SW-RFA or single-monopolar RFA (S-RFA). Read More

View Article and Full-Text PDF

Determination of initial airtightness after anatomical laser segmentectomy in an ex vivo model.

Lasers Med Sci 2021 Apr 23. Epub 2021 Apr 23.

Department of Surgery, Asklepios Stadtklinik Bad Wildungen, Bad Wildungen, Germany.

If a pulmonary pathology can be removed by anatomical segmentectomy, the need for lobectomy is obviated. The procedure is considered oncologically equivalent and saves healthy lung tissue. In every segmentectomy, lung parenchyma must be transected in the intersegmental plane. Read More

View Article and Full-Text PDF

Radiofrequency ablation for markedly incompetent perforators versus compression therapy in the management of post-phelebtic venous ulcers: A randomized controlled trial.

Vascular 2021 Apr 22:17085381211010022. Epub 2021 Apr 22.

Department of Vascular and Endovascular Surgery, Mansoura University Hospitals, Mansoura University, Mansoura, Egypt.

Background: Venus ulcers developed mainly due to reflux of incompetent venous valves in perforating veins.

Patients And Methods: In this randomized controlled trial, 119 patients recruited over two years, with post-phelebtic venous leg ulcers, were randomly assigned into one of two groups: either to receive radiofrequency ablation of markedly incompetent perforators (Group A, n = 62 patients) or to receive conventional compression therapy (Group B, n = 57 patients). Follow-up duration required for ulcer healing continued for 24 months post randomization. Read More

View Article and Full-Text PDF

Persistent Visual and Vestibular Impairments for Postural Control Following Concussion: A Cross-Sectional Study in University Students.

Sports Med 2021 Apr 21. Epub 2021 Apr 21.

Department of Kinesiology & Applied Physiology and Interdisciplinary Biomechanics and Movement Science Program, University of Delaware, Newark, DE, 19713, USA.

Objective: To examine how concussion may impair sensory processing for control of upright stance.

Methods: Participants were recruited from a single university into 3 groups: 13 participants (8 women, 21 ± 3 years) between 2 weeks and 6 months post-injury who initiated a return-to-play progression (under physician management) by the time of testing (recent concussion group), 12 participants (7 women, 21 ± 1 years) with a history of concussion (concussion history group, > 1 year post-injury), and 26 participants (8 women, 22 ± 3 years) with no concussion history (control group). We assessed sensory reweighting by simultaneously perturbing participants' visual, vestibular, and proprioceptive systems and computed center of mass gain relative to each modality. Read More

View Article and Full-Text PDF

Exploring the potential of rapid evaporative ionization mass spectrometry (Intelligent Knife) for point-of-care testing in aortic surgery.

Eur J Cardiothorac Surg 2021 Apr 11. Epub 2021 Apr 11.

Liverpool Centre for Cardiovascular Science, Liverpool, UK.

Objectives: Many intraoperative decisions regarding the extent of thoracic aortic surgery are subjective and are based on the appearance of the aorta, perceived surgical risks and likelihood of early recurrent disease. Our objective in this work was to carry out a cross-sectional study to demonstrate that rapid evaporative ionization mass spectrometry (REIMS) of electrosurgical aerosol is able to empirically discriminate ex vivo aneurysmal human thoracic aorta from normal aorta, thus providing supportive evidence for the development of the technique as a point-of-care test guiding intraoperative surgical decision-making.

Methods: Human aortic tissue was obtained from patients undergoing surgery for thoracic aortic aneurysms (n = 44). Read More

View Article and Full-Text PDF

COMBINED BIPOLAR AND V-SHAPED LESIONS FOR LUMBAR FACET RADIOFREQUENCY ABLATION: A TECHNICAL REPORT.

Pain Med 2021 Apr 8. Epub 2021 Apr 8.

Department of Pain Management, Cleveland Clinic, Cleveland, OH.

Introduction: Radiofrequency ablation (RFA) is a demonstrated safe and effective treatment for chronic low back pain. RFA delivers high density electrical current near lumbar medial branch nerve. The success of RFA is contingent upon creating a lesion that overlaps the sensory nerve supplying the affected facet joint(s). Read More

View Article and Full-Text PDF

Direct Cortical Stimulation in Neurosurgical Emergencies: Single-Center Experience in 2 Patients.

World Neurosurg 2021 Apr 2;150:147-152. Epub 2021 Apr 2.

Department of Neurosurgery, University Hospital Düsseldorf, Düsseldorf, Germany; Centre of Neurooncology, University Hospital Düsseldorf, Düsseldorf, Germany.

Background: Intraoperative neuromonitoring (IONM) is widely used for elective resection of eloquently located brain tumors to increase safety and extent of resection. Owing to the need for specially trained personnel for IONM and the sophisticated, time-consuming technical setup, standard IONM is usually not suitable for emergency situations. We report the use of a device that can be operated by the neurosurgeon autonomously for monopolar brain mapping in 2 emergency cases. Read More

View Article and Full-Text PDF

Comparison of wire and disc electrodes to electrically activate the inspiratory muscles in dogs.

J Neurosci Methods 2021 Jun 2;357:109176. Epub 2021 Apr 2.

Department of Physical Medicine and Rehabilitation, Case Western Reserve University, 2500 MetroHealth Drive, Cleveland, OH, USA; MetroHealth Research Institute, MetroHealth Medical Center, 2500 MetroHealth Drive, Cleveland, OH, USA. Electronic address:

Objective: To compare the effectiveness of wire versus disc electrodes to activate the inspiratory muscles via high frequency spinal cord stimulation.

Design: Animal study.

Setting: Research laboratory. Read More

View Article and Full-Text PDF

Right-sided Zenker's diverticulum resected using intraoperative neuromonitoring.

Clin J Gastroenterol 2021 Apr 4. Epub 2021 Apr 4.

Department of Surgical Oncology, Gifu University School of Medicine, 1-1 Yanagido, Gifu, 501-1194, Japan.

Right-sided Zenker's diverticulum is a rare pharyngoesophageal diverticulum. The risk of intraoperative injury of the recurrent laryngeal nerve is high during transcervical diverticulectomy because this nerve presents many variations of extralaryngeal bifurcation before entry into the larynx. We present a case of right-sided Zenker's diverticulum that was safely resected with the use of intraoperative neuromonitoring to prevent right recurrent laryngeal nerve injury. Read More

View Article and Full-Text PDF

Creatine promotes cancer metastasis through activation of Smad2/3.

Cell Metab 2021 Mar 27. Epub 2021 Mar 27.

Key Laboratory of RNA Biology, Key Laboratory of Protein and Peptide Pharmaceutical, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China; College of Life Sciences, University of Chinese Academy of Sciences, Beijing 100049, China; Center for Excellence in Biomacromolecules, Chinese Academy of Sciences, Beijing 100101, China. Electronic address:

As one of the most popular nutrient supplements, creatine has been highly used to increase muscle mass and improve exercise performance. Here, we report an adverse effect of creatine using orthotopic mouse models, showing that creatine promotes colorectal and breast cancer metastasis and shortens mouse survival. We show that glycine amidinotransferase (GATM), the rate-limiting enzyme for creatine synthesis, is upregulated in liver metastases. Read More

View Article and Full-Text PDF

Validation of Ultrasonic Harmonic Scalpel for Real-Time Tissue Identification Using Rapid Evaporative Ionization Mass Spectrometry.

Anal Chem 2021 04 31;93(14):5906-5916. Epub 2021 Mar 31.

Department of Metabolism, Digestion and Reproduction, Imperial College London, South Kensington Campus, London SW7 2AZ, United Kingdom.

In this study, we integrate rapid evaporative ionization mass spectrometry (REIMS) with the Harmonic scalpel, an advanced laparoscopic surgical instrument that utilizes ultrasound energy to dissect and coagulate tissues. It provides unparalleled manipulation capability to surgeons and has superseded traditional electrosurgical tools particularly in abdominal surgery, but is yet to be validated with REIMS. The REIMS platform coupled with the Harmonic device was shown to produce tissue-specific lipid profiles through the analysis of porcine tissues in both negative and positive ionization modes. Read More

View Article and Full-Text PDF

En-bloc resection of urinary bladder tumour - a prospective controlled multicentre observational study.

Wideochir Inne Tech Maloinwazyjne 2021 Mar 15;16(1):145-150. Epub 2020 May 15.

Second Department of Urology, Centre of Postgraduate Medical Education, Warsaw, Poland.

Introduction: Transurethral resection of bladder tumour (TURBT) is one of the most commonly performed urologic procedures. Because of the shortcomings of conventional TURBT, the en-bloc resection concept was created.

Aim: To analyse the influence of en-bloc technique on surgical and oncological outcomes of TURBT performed with electric current. Read More

View Article and Full-Text PDF

Selective Extraction of Medium-Chain Carboxylic Acids by Electrodialysis and Phase Separation.

ACS Omega 2021 Mar 12;6(11):7841-7850. Epub 2021 Mar 12.

Advanced Water Management Centre, The University of Queensland, Brisbane, Queensland 4072, Australia.

Carboxylic acids obtained the microbial electrochemical conversion of waste gases containing carbon dioxide (, microbial electrosynthesis) can be used of nonrenewable building-block chemicals in the manufacture of a variety of products. When targeting valuable medium-chain carboxylic acids such as caproic acid, electricity-driven fermentations can be limited by the accumulation of fermentation products in the culturing media, often resulting in low volumetric productivities and titers due to direct toxicity or inhibition of the biocatalyst. In this study, we tested the effectiveness of a simple electrodialysis system in upconcentrating carboxylic acids from a model solution mimicking the effluent of a microbial electrochemical system producing short- and medium-chain carboxylic acids. Read More

View Article and Full-Text PDF

Inhibition of 14-3-3 Proteins Alters Neural Oscillations in Mice.

Front Neural Circuits 2021 12;15:647856. Epub 2021 Mar 12.

Department of Biomedical Sciences, Florida State University College of Medicine, Tallahassee, FL, United States.

Accumulating evidence suggests that schizophrenia is a disorder of the brain's communication, a result of functional and structural dysconnectivities. Patients with schizophrenia exhibit irregular neuronal circuit and network activity, but the causes and consequences of such activity remain largely unknown. Inhibition of 14-3-3 proteins in the mouse brain leads to the expression of multiple schizophrenia endophenotypes. Read More

View Article and Full-Text PDF

Computational exploration of epidural cortical stimulation using a realistic head model.

Comput Biol Med 2021 Mar 25:104290. Epub 2021 Mar 25.

School of Electrical Engineering and Computer Science, Gwangju Institute of Science & Technology, South Korea. Electronic address:

Motor cortex stimulation, either non-invasively or with implanted electrodes, has been applied worldwide as a treatment for intractable neuropathic pain syndromes. Although computer simulations of non-invasive brain stimulation have been investigated largely to optimize protocols and improve our understanding of underlying mechanisms using a realistic head model, computational studies of invasive cortical stimulation are rare and limited to very simplified cortical models. In this paper, we present an anatomically realistic head model for epidural cortical stimulation that includes the most sophisticated epidural electrodes with an insulating paddle. Read More

View Article and Full-Text PDF

Systemic methylprednisolone for hearing preservation during cochlear implant surgery: A double blinded placebo-controlled trial.

Hear Res 2021 May 28;404:108224. Epub 2021 Feb 28.

Department of Surgery - Otolaryngology, The University of Melbourne, Royal Victorian Eye and Ear Hospital, 32 Gisborne St RVEEH, Melbourne East 3002,Victoria, Australia; Cochlear Implant Clinic, Royal Victorian Eye and Ear Hospital, 32 Gisborne St, Melbourne East 3002, Australia.

Aim: To assess whether a single, peri-operative, high dose of methylprednisolone can improve the preservation of residual acoustic hearing following cochlear implantation (CI).

Methods: This was a double blinded placebo-controlled trial, performed in a tertiary academic centre. The hypothesis was that methylprednisolone would improve the preservation of hearing, and lower electrode impedances. Read More

View Article and Full-Text PDF