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Braz J Microbiol 2021 Apr 10. Epub 2021 Apr 10.

Beijing Key Laboratory of Seed Disease Testing and Control, Department of Plant Pathology, China Agricultural University, Beijing, 100193, People's Republic of China.

Guanosine 5'-diphosphate-3'-diphosphate (ppGpp) is a small molecule nucleotide alarmone that can accumulate under the amino acid starvation state and trigger the stringent response. This study reported the extraction of ppGpp from the Gram-positive bacteria Clavibacter michiganensis through methods using formic acid, lysozyme, or methanol. Following extraction, ppGpp was detected through ultra-high-performance liquid chromatography (UHPLC) and liquid chromatography-tandem mass spectrometry (LC-MS/MS). Read More

Cancer Immunol Immunother 2021 Apr 10. Epub 2021 Apr 10.

Trion Research GmbH, Am Klopferspitz 19, 82152, Martinsried, Germany.

Transurethral resection of the tumor (TUR-B) followed by adjuvant intravesical treatment with cytostatic drugs or Bacillus Calmette-Guérin (BCG) as standard therapy of non-muscle-invasive bladder cancer (NMIBC) is associated with a high recurrence rate of about 60-70%, considerable side effects and requires close monitoring. Alternative treatment options are warranted. Two patients with epithelial cell adhesion molecule (EpCAM)-positive recurrent non-muscle invasive bladder cancer were treated the first time by an intravesical administration of the trifunctional bispecific EpCAM targeting antibody catumaxomab (total dosage of 470 and 1120 µg, respectively). Read More

Bone 2021 Apr 6:115945. Epub 2021 Apr 6.

Division of Diagnostic and Surgical Sciences, UCLA School of Dentistry, Los Angeles, CA, USA. Electronic address:

Medication related osteonecrosis of the Jaws (MRONJ) is a severe complication of antiresorptive and anti-angiogenic medications. Osteoclast inhibition is central in MRONJ pathogenesis. Here, we investigated if local application of RANKL (a key molecule in osteoclast activation) could enhance osteoclast generation and improve extraction socket healing in the presence of bisphosphonates. Read More

Nucleic Acids Res 2021 Apr 9. Epub 2021 Apr 9.

Institut de Biologie de l'Ecole Normale Supérieure (IBENS), Ecole Normale Supérieure, CNRS, INSERM, Université PSL, 46 rue d'Ulm, F-75005 Paris, France.

The replication strategy of metazoan genomes is still unclear, mainly because definitive maps of replication origins are missing. High-throughput methods are based on population average and thus may exclusively identify efficient initiation sites, whereas inefficient origins go undetected. Single-molecule analyses of specific loci can detect both common and rare initiation events along the targeted regions. Read More

Methods Mol Biol 2021 ;2284:531-541

Department of Computational Biology and Medical Sciences, Graduate School of Frontier Sciences, The University of Tokyo, Kashiwa-shi, Chiba, Japan.

RNA-seq using long-read sequencing, such as nanopore and SMRT (Single Molecule, Real-Time) sequencing, enabled the identification of the full-length structure of RNA molecules. Several tools for long-read RNA-seq were developed recently. In this section, we introduce an analytical pipeline of long-read RNA-seq for isoform identification and the estimation of expression levels using minimap2, TranscriptClean, and TALON. Read More

J Biomed Mater Res B Appl Biomater 2021 Apr 8. Epub 2021 Apr 8.

Department of Dental Sciences, University Institute of Health Sciences (IUCS), CESPU, Gandra, PRD, Portugal.

The main aim of this study was to perform an integrative review on the release of bisphenol A (BPA) from resin-matrix composites and potential toxic effects. A bibliographic search was performed on the PubMed platform using the following keywords: "Bisphenol A" OR "BPA" AND "resin composite" OR "composite resin" AND "toxicity" OR "cytotoxicity" OR "release". Inclusion criteria involved in vitro and in vivo studies on the release and toxicity of BPA. Read More

Life Sci 2021 Apr 5:119398. Epub 2021 Apr 5.

Department of Gastrointestinal Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China. Electronic address:

Background: Recent studies have provided compelling evidence regarding the association of microRNAs (miRNAs) with the progression and development of tumors. Among the miRNAs, the dysregulation of miR-146b-3p expression has been reported in several cancers, however, its effect on colorectal cancer (CRC) remains unexplored. Many studies have suggested a close correlation between the transcription factor (TF)-miRNA signal and cancer. Read More

PLoS One 2021 8;16(4):e0249797. Epub 2021 Apr 8.

Metabolon, Morrisville, North Carolina, United States of America.

Introduction: Analysis of blood for the evaluation of clinically relevant biomarkers requires precise collection and sample handling by phlebotomists and laboratory staff. An important consideration for the clinical application of metabolomics are the different anticoagulants utilized for sample collection. Most studies that have characterized differences in metabolite levels in various blood collection tubes have focused on single analytes. Read More

Genes Genomics 2021 Apr 8. Epub 2021 Apr 8.

Clinical Laboratory, The First Hospital of Yulin, No. 59 Yuxi Avenue, Yulin, 719000, Shaanxi, China.

Background: A growing number of studies have shown that circular RNA (circRNA) is an important regulator molecule in cancer progression, but it has been poorly studied in diffuse large b-cell lymphoma (DLBCL).

Objective: This study aimed to explore the role of circ_OTUD7A in DLBCL.

Methods: Relative expression levels of circ_OTUD7A, microRNA (miR)-431-5p and forkhead box P1 (FOXP1) were determined by quantitative real-time PCR (qRT-PCR). Read More

Nature 2021 Apr 7;592(7853):205-208. Epub 2021 Apr 7.

INAF-Osservatorio Astrofisico di Arcetri, Arcetri, Italy.

The atmospheres of gaseous giant exoplanets orbiting close to their parent stars (hot Jupiters) have been probed for nearly two decades. They allow us to investigate the chemical and physical properties of planetary atmospheres under extreme irradiation conditions. Previous observations of hot Jupiters as they transit in front of their host stars have revealed the frequent presence of water vapour and carbon monoxide in their atmospheres; this has been studied in terms of scaled solar composition under the usual assumption of chemical equilibrium. Read More

G3 (Bethesda) 2021 Feb 5. Epub 2021 Feb 5.

Department of Viticulture and Enology, University of California Davis, Davis, CA 95616, USA.

Muscadinia rotundifolia, the muscadine grape, has been cultivated for centuries in the southeastern United States. M. rotundifolia is resistant to many of the pathogens that detrimentally affect Vitis vinifera, the grape species commonly used for winemaking. Read More

J Bone Miner Metab 2021 Apr 5. Epub 2021 Apr 5.

Shengli Clinical Medical College of Fujian Medical University, Fuzhou, 350001, Fujian, China.

Introduction: PTH1-34 can stimulate osteoblast formation, which contributes to the improvement of bone loss. PTH1-34 can activate autophagy, and autophagy plays a key role in osteoblast formation. This study aimed to explore the role of autophagy in PTH1-34-regulated osteoblastogenesis. Read More

Heliyon 2021 Mar 21;7(3):e06527. Epub 2021 Mar 21.

Department of Chemistry, St Berchmans College (Autonomous), Changanassery, 686101, Kerala, India.

Single crystals of Sodium Oxalate (SO) were grown by adopting the slow evaporation solution growth approach from aqueous solution. The prominent functional groups seen in the SO crystal were distinctly detected with Fourier transform infrared and FT-Raman spectral analysis. The cut-off wavelength of 230 nm was measured using Ultraviolet -visible spectral analysis. Read More

Open Life Sci 2019 Jan 15;14:454-461. Epub 2019 Nov 15.

Department of Radiotherapy, The Second Hospital of Jilin University, Changchun, 130041, Jilin, China.

Targeting 6-phosphogluconate dehydrogenase (6PGD) can inhibit cancer cell proliferation and tumor growth. However, the relationship between 6PGD and cisplatin resistance still needs further study. Cisplatin-sensitive and cisplatin-resistant ovarian cancer OV2008 and C13* lines and lung cancer A549 and A549DDP lines were treated with different concentrations of cisplatin and cell viability was evaluated. Read More

Biomed Res Int 2021 16;2021:5612138. Epub 2021 Mar 16.

Department of Clinical Laboratory, Beijing You'an Hospital, Capital Medical University, Beijing 100069, China.

Purpose: Interferon-induced transmembrane protein 3 (IFITM3) is a key signaling molecule regulating cell growth in some tumors, but its function and mechanism in hepatocellular carcinoma (HCC) remain unknown. Our study investigated the relationship between the expression of IFITM3 and HCC development. . Read More

Front Pharmacol 2021 18;12:641435. Epub 2021 Mar 18.

Xianyang Central Hospital of Shaanxi Province, Xianyang, China.

Lumbar spinal stenosis (LSS) is a spinal degenerative disease, complicated with nerve injury. Lysophosphatidic acid (LPA), a kind of glycerophospholipid molecule is elevated in the initial stages of neural injury. This research aimed to investigate the patho-mechanism of nerve injury caused by LPA in LSS patients. Read More

Hypertension 2021 Apr 5:HYPERTENSIONAHA12016252. Epub 2021 Apr 5.

Barrow Aneurysm and AVM Research Center, Barrow Neurological Institute, Phoenix, AZ. (M.K., J.P., Y.K., K.M., H.F., K.Y., T.M., Hitomi Sato, Hiroki Sato, J.A., M.T.L., T.H.).

Potential roles for neutrophils in the pathophysiology of intracranial aneurysm have long been suggested by clinical observations. The presence of neutrophil enzymes in the aneurysm wall has been associated with significant increases in rupture risk. However, the mechanisms by which neutrophils may promote aneurysm rupture are not well understood. Read More

Zhongguo Shi Yan Xue Ye Xue Za Zhi 2021 Apr;29(2):403-407

College of Engineering, Beijing Normal University, Zhuhai 519087, Guangdong Province, China,E-mail:

Objective: To establish a method for rapid detection and typing of NPM1 mutations in acute myeloid leukemia (AML) by fluorescence melting curve analysis technology.

Methods: A pair of primers and a fluorescent single-stranded probe (molecule beacon) were designed for the mutant genes mutA, mutB, mutD in exon 12 of nucleopsin (NPM1) and wild type. With a real-time qPCR, the A, B, and D gene mutations of NPM1 were detected and typed by different-melting curve peak value of the probe through RT-PCR. Read More

J Cell Mol Med 2021 Apr 3. Epub 2021 Apr 3.

Department of Radiation Oncology, The Affiliated Lihuili Hospital, Ningbo University, Ningbo, China.

Pinin (PNN) was originally characterized as a desmosome-associated molecule. Its function and the mechanism of its regulation in renal cell carcinoma (RCC) are still undefined. Data on PNN expression, clinicopathological features, and prognosis of patients with RCC were obtained from The Cancer Genome Atlas (TCGA) database. Read More

Int J Mol Sci 2021 Mar 22;22(6). Epub 2021 Mar 22.

Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand.

Alternansucrase (ALT, EC 2.4.1. Read More

Nanomaterials (Basel) 2021 Mar 19;11(3). Epub 2021 Mar 19.

Institute of Science and Industrial Research, Osaka University, 8-1 Mihogaoka, Ibaraki, Osaka 567-0047, Japan.

Cyclic adenosine monophosphate (cAMP) is an important research target because it activates protein kinases, and its signaling pathway regulates the passage of ions and molecules inside a cell. To detect the chemical reactions related to the cAMP intracellular signaling pathway, cAMP, adenosine triphosphate (ATP), adenosine monophosphate (AMP), and adenosine diphosphate (ADP) should be selectively detected. This study utilized single-molecule quantum measurements of these adenosine family molecules to detect their individual electrical conductance using nanogap devices. Read More

Plants (Basel) 2021 Mar 30;10(4). Epub 2021 Mar 30.

School of Design, Shanghai Jiao Tong University, Shanghai 200240, China.

This study aimed to obtain the full-length transcriptome of purslane (); assorted plant samples were used for single-molecule real-time (SMRT) sequencing. Based on SMRT, functional annotation of transcripts, transcript factors (TFs) analysis, simple sequence repeat analysis and long non-coding RNAs (LncRNAs) prediction were accomplished. Total 15. Read More

Cancers (Basel) 2021 Mar 5;13(5). Epub 2021 Mar 5.

Koltzov Institute of Developmental Biology of Russian Academy of Sciences, 26 Vavilov Street, 119334 Moscow, Russia.

A special problem in the surgery of rectal cancer is connected with a need for appropriate removal of intestine parts, along with the tumor, including the fragment close to the sphincter. To determine the length of fragments to remove, it is necessary to reveal areas without changes in molecule functioning, specific for tumor. The purpose of the present study was to investigate functioning the proteasomes, the main actors in protein hydrolysis, in patient rectal adenocarcinoma and different intestine locations. Read More

Sensors (Basel) 2021 Mar 4;21(5). Epub 2021 Mar 4.

School of Microelectronics, Southern University of Science and Technology, Shenzhen 518055, China.

Polydimethylsiloxane (PDMS) is a polymer widely used for fabrication and prototyping of microfluidic chips. The porous matrix structure of PDMS allows small hydrophobic molecules including some fluorescent dyes to be readily absorbed to PDMS and results in high fluorescent background signals, thereby significantly decreasing the optical detection sensitivity. This makes it challenging to accurately detect the fluorescent signals from samples using PDMS devices. Read More

Int J Mol Sci 2021 Mar 17;22(6). Epub 2021 Mar 17.

Institute of Biomechanics and Orthopaedics, German Sport University Cologne, 50933 Cologne, Germany.

Osteoarthritis (OA) is a multifactorial disease which is characterized by a change in the homeostasis of the extracellular matrix (ECM). The ECM is essential for the function of the articular cartilage and plays an important role in cartilage mechanotransduction. To provide a better understanding of the interaction between the ECM and the actin cytoskeleton, we investigated the localization and expression of the Ca-dependent proteins cartilage oligomeric matrix protein (COMP), thrombospondin-1 (TSP-1), plastin 3 (PLS3) and stromal interaction molecule 1 (STIM1). Read More

Int J Mol Sci 2021 Mar 17;22(6). Epub 2021 Mar 17.

Department of Biomedical Science, Jungwon University, 85 Munmu-ro, Goesan-eup, Goesan-gun, Chungbuk 28024, Korea.

Coxsackievirus and adenovirus receptor (CAR) is present in epithelial and vascular endothelial cell junctions. We have previously shown a hemorrhagic phenotype in germ-line CAR knock-out mouse embryos; we have also found that CAR interacts with ZO-1 and β-catenin. However, the role of CAR in vascular endothelial junction permeability has not been proven. Read More

Sensors (Basel) 2021 Mar 3;21(5). Epub 2021 Mar 3.

NanoBio High-Tech Materials Research Center, Department of Biological Engineering, Inha University, 100 Inha-ro, Incheon 22212, Korea.

Meat is often wasted due to the perceived concerns of its shelf life and preservation. Specifically, in meat formation, biogenic amines (BAs) are the major agents to spoil them. Herein, we have developed a carbon disulfide (CS) added colloidal gold nanoparticles-based colorimetric sensor for the rapid and on-site detection of biogenic amines. Read More

Cancers (Basel) 2021 Mar 3;13(5). Epub 2021 Mar 3.

Radiotherapy and OncoImmunology Laboratory, Department of Radiation Oncology, Radboud University Medical Center, 6525 GA Nijmegen, The Netherlands.

To study head and neck squamous cell carcinomas (HNSCC) in vitro, a large variety of HNSCC cell lines have been developed. Here, we characterize a panel of 22 HNSCC cell lines, thereby providing a tool for research into tumor-specific treatment options in HNSCC. Both human papillomavirus (HPV) positive and HPV negative tumor cell lines were collected from commercial and collaborative sources. Read More

Plants (Basel) 2021 Mar 10;10(3). Epub 2021 Mar 10.

Department of Botany, Aristotle University of Thessaloniki, GR-54124 Thessaloniki, Greece.

, a fungal pathogen that causes gray mold, is damaging more than 200 plant species, and especially tomato. Photosystem II (PSII) responses in tomato ( L.) leaves to spore suspension application were evaluated by chlorophyll fluorescence imaging analysis. Read More

Int J Mol Sci 2021 Mar 10;22(6). Epub 2021 Mar 10.

Department of Biochemistry, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.

Although epoxyeicosatrienoic acid (EET) analogs have performed well in several acute and chronic kidney disease models, targeted delivery of EET analogs to the kidney can be reasonably expected to reduce the level of drug needed to achieve a therapeutic effect and obviate possible side effects. For EET analog kidney-targeted delivery, we conjugated a stable EET analog to folic acid via a PEG-diamine linker. Next, we compared the kidney targeted EET analog, EET-F01, to a well-studied EET analog, EET-A. Read More