316,168 results match your criteria molecular level

Recent advances in demystifying the metabolism of lipoprotein(a).

Atherosclerosis 2022 May;349:82-91

Medical School, University of Western Australia, Perth, WA, Australia; Lipid Disorders Clinic, Department of Cardiology and Internal Medicine, Royal Perth Hospital, Perth, Western Australia, Australia. Electronic address:

Lipoprotein (a) (Lp(a)) is a strange lipoprotein species causatively independently and significantly associated with cardiovascular diseases and calcified aortic valve stenosis. Elevated plasma Lp(a) levels increase the rate of cardiovascular events at any achieved low-density lipoprotein (LDL) level. The major structural difference between Lp(a) and LDL is that Lp(a) has a second large protein, apolipoprotein (a) (apo(a)), bound to the apolipoprotein B100 moiety of an LDL sized particle by a single disulfide bond. Read More

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Putting in the Erk: Growth factor signaling and mesoderm morphogenesis.

Curr Top Dev Biol 2022 4;149:263-310. Epub 2022 Mar 4.

Department of Molecular Biology, Princeton University, Princeton, NJ, United States. Electronic address:

It has long been known that FGF signaling contributes to mesoderm formation, a germ layer found in triploblasts that is composed of highly migratory cells that give rise to muscles and to the skeletal structures of vertebrates. FGF signaling activates several pathways in the developing mesoderm, including transient activation of the Erk pathway, which triggers mesodermal fate specification through the induction of the gene brachyury and activates morphogenetic programs that allow mesodermal cells to position themselves in the embryo. In this review, we discuss what is known about the generation and interpretation of transient Erk signaling in mesodermal tissues across species. Read More

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Mol Pharmacol 2022 May 23. Epub 2022 May 23.

Molecular and Cellular Biology, Kennesaw State University, United States

CBL0137 is a lead drug for human African trypanosomiasis, caused by Herein, we use a four-step strategy to (a) identify physiologic targets and (b) determine modes of molecular action of CBL0137 in the trypanosome. First, we identified fourteen CBL0137-binding proteins using affinity chromatography. Second, we developed hypotheses of molecular modes of action, using predicted functions of CBL0137-binding proteins as guides. Read More

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Effects of wastewater-spiked nanoparticles of silver and titanium dioxide on survival, growth, reproduction and biochemical markers of Daphnia magna.

Sci Total Environ 2022 May 20:156079. Epub 2022 May 20.

Department of Biology & CESAM, University of Aveiro, Campus Universitário de Santiago, 3810-193 Aveiro, Portugal. Electronic address:

Silver (Ag) and titanium dioxide (TiO) nanoparticles (NPs) are released into aquatic environments through wastewater treatment plants (WWTPs). Even though these NPs are mostly retained in WWTPs, a small fraction can be found in released effluents and may exert toxic effects on aquatic biota. Currently, the available information about the sublethal effects of wastewater-borne NPs on aquatic organisms is inconclusive and the importance of exposure media remains poorly understood. Read More

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Negative impacts of sea-level rise on soil microbial involvement in carbon metabolism.

Sci Total Environ 2022 May 20:156087. Epub 2022 May 20.

School of Life Sciences/State Key Laboratory of Biocontrol, Sun Yat-sen University, Guangzhou 510275, China; Research Institute of Sun Yat-sen University in Shenzhen, Shenzhen 518057, China. Electronic address:

Sea-level rise has been threatening the terrestrial ecosystem functioning of coastal islands, of which the most important component is carbon (C) cycling. However, metagenomic and metabolomic evidence documenting salt intrusion effects on molecular biological processes of C cycling are still lacking. Here, we investigated microbial communities, metagenomic taxonomy and function, and metabolomic profiles in the marine-terrestrial transition zone of low- and high-tide, and low- and high-land areas based on distances of 0 m, 50 m, 100 m, and 200 m, respectively, to the water-land junction of Neilingding Island. Read More

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Current and future diagnostic and treatment strategies for patients with invasive lobular breast cancer.

Ann Oncol 2022 May 20. Epub 2022 May 20.

Laboratory for Translational Breast Cancer Research (LTBCR), Department of Oncology, KU Leuven, 3000 Leuven, Belgium. Electronic address:

Background: Invasive Lobular breast cancer (ILC) is the second most common type of breast cancer after invasive breast cancer of no special type (NST), representing up to 15% of all breast cancers.

Design: Latest data on ILC are presented, focusing on diagnosis, molecular make-up according to the ESMO Scale for Clinical Actionability of molecular Targets (ESCAT) guidelines, treatment in the early and metastatic setting and ILC-focused clinical trials.

Results: At the imaging level, MRI-based and novel PET/CT-based techniques can overcome the limitations of currently used imaging techniques for diagnosing ILC. Read More

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Oligo-layer graphene stabilized fully exposed Fe-sites for ultra-sensitivity electrochemical detection of dopamine.

Biosens Bioelectron 2022 May 17;211:114367. Epub 2022 May 17.

Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology (Ministry of Education), Department of Chemistry, Center for Synthetic and Systems Biology, Tsinghua University, Beijing, 100084, China. Electronic address:

Neurotransmitter dopamine (DA) has been implicated in a variety of physiological and pathological processes, realizing its low detection limit and high sensitivity analysis is of great significance for early disease diagnosis. Herein, we propose a simple pyrolysis approach for dispersing Fe-sites onto the N-doped graphene support (denoted as Fe/N-GR) to construct an electrochemical biosensor for DA detection. The fully exposed Fe-sites guaranteed the well-defined active center for electrochemical oxidation of DA. Read More

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Rapid qualitative and quantitative analysis of trace aconitum phytotoxin by SERS.

Food Chem 2022 May 18;391:133234. Epub 2022 May 18.

State Key Laboratory for Physical Chemistry of Solid Surfaces, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China.

The dual functions of phytotoxin, such as aconitine, with biological activity and toxicity ignited the related food poisoning intentionally or accidentally from time to time. The fast and accurate qualitative analysis is a prerequisite for tracking the source of poisoning and taking correct treatments. Taking the single molecule level sensitivity and molecular fingerprinting of Surface-enhanced Raman spectroscopy (SERS), we developed a highly sensitive and accurate strategy for the trace detection of three structurally similar aconitines (ATs) (aconitine, mesaconitine and hypoaconitine) by employing the 100 nm Ag NPs colloid as the SERS substrate. Read More

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Electroporation, electrochemotherapy and electro-assisted drug delivery in cancer. A state-of-the-art review.

Biophys Chem 2022 Apr 15;286:106819. Epub 2022 Apr 15.

Institute of Biophysics and Biomedical Engineering, Bulgarian Academy of Sciences, Acad. G. Bonchev Str., Bl. 21, Sofia 1113, Bulgaria. Electronic address:

This review focuses on electrochemotherapy that consists in the delivery of anti-cancer drugs using high-voltage electrical pulses. Technical issues, choice of drugs, and protocol of drug delivery are still under investigation and no consensus has been achieved yet. The different aspects of electrochemotherapy are discussed in the present paper. Read More

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Enhancement in chaperone activity of human αA-crystallin by nanochaperone gold nanoparticles: Multispectroscopic studies on their molecular interactions.

Spectrochim Acta A Mol Biomol Spectrosc 2022 May 13;279:121344. Epub 2022 May 13.

Department of Chemistry, National Institute of Technology Hamirpur, Himachal Pradesh 177005, India. Electronic address:

The chaperone activity of human αA-crystallin (HAA) against aggregation of human γD-crystallin (HGD) was enhanced by gold nanoparticles (AuNPs). Chaperone activity of HAA was almost doubled in the presence of 5.5 nM gold nanoparticles (AuNPs). Read More

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Long-term exposure to ozone and cardiovascular mortality in a large Chinese cohort.

Environ Int 2022 May 17;165:107280. Epub 2022 May 17.

Department of Epidemiology and Biostatistics, School of Public Health, Peking University, No. 38 Xueyuan Road, Haidian District, Beijing 100191, China; Center for Real-World Evidence Evaluation, Peking University Clinical Research Institute, No. 38 Xueyuan Road, Haidian District, Beijing 100191, China; Key Laboratory of Molecular Cardiovascular (Peking University), Ministry of Education, Beijing, China. Electronic address:

Background: Evidence for the association between long-term exposure to ozone (O) and cause-specific cardiovascular disease (CVD) mortality is inconclusive, and this association has rarely been evaluated at high O concentrations.

Objectives: We aim to evaluate the associations between long-term O exposure and cause-specific CVD mortality in a Chinese population.

Methods: From 2009 to 2018, 744,882 subjects (median follow-up of 7. Read More

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HATs meet structural biology.

Curr Opin Struct Biol 2022 May 20;74:102389. Epub 2022 May 20.

Institute for Research in Biomedicine, The Barcelona Institute of Science and Technology (BIST), Baldiri I Reixac 10, E-08028, Barcelona, Spain; The Spanish CIBER of Rare Diseases (CIBERER), ISCIII. U-731, E-08028, Barcelona, Spain; Department of Biochemistry and Molecular Biomedicine, Universitat de Barcelona, Spain. Electronic address:

Heteromeric amino acid transporters (HATs) are one of the ten types of amino acid transporters present in the human body. Growing interest in the pathophysiological role of this group of transporters in rare and complex diseases and cancer has brought about the recent resolution of various structures of human HATs and bacterial homologues at atomic level. This knowledge sheds light on the mechanisms of transport used by these molecules. Read More

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Dynamics of Photogenerated Charge Carriers in Inorganic/Organic S-Scheme Heterojunctions.

J Phys Chem Lett 2022 May 23:4695-4700. Epub 2022 May 23.

Laboratory of Solar Fuel, Faculty of Materials Science and Chemistry, China University of Geosciences, Wuhan, Hubei 430074, China.

Step-scheme heterojunctions formed between two firmly bound photocatalysts facilitate charge separation due to interfacial charge transfer, which is usually illustrated by the gain or loss of electrons in the constituent photocatalysts characterized by in situ irradiated X-ray photoelectron spectroscopy. This technique provides a steady-state view of charge distribution but overlooks the transient and complex dynamics of charge transfer, trapping, and recombination. To provide a molecular-level and dynamic view of these processes, we investigated the behaviors of photogenerated charge carriers within an inorganic/organic TiO/polydopamine S-scheme heterojunction using ultrafast transient absorption spectroscopy and time-resolved photoluminescence spectroscopy. Read More

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Differential Cross Sections for Cold, State-to-State Spin-Orbit Changing Collisions of NO( = 10) with Neon.

J Phys Chem A 2022 May 23. Epub 2022 May 23.

Department of Chemistry, University of Missouri, Columbia, Missouri 65211, United States.

Inelastic scattering processes have proven a powerful means of investigating molecular interactions, and much current effort is focused on the cold and ultracold regime where quantum phenomena are clearly manifested. Studies of collisions of the open shell nitric oxide (NO) molecule have been central in this effort since the pioneering work of Houston and co-workers in the early 1990s. State-to-state scattering of vibrationally excited molecules in the cold regime introduces challenges that test the suitability of current theoretical methods for determination of intermolecular potentials, and concomitant electronically nonadiabatic processes raise the bar further. Read More

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Design principles of PI(4,5)P clustering under protein-free conditions: Specific cation effects and calcium-potassium synergy.

Proc Natl Acad Sci U S A 2022 May 23;119(22):e2202647119. Epub 2022 May 23.

Laboratory of Computational Biology, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD 20892.

SignificanceClustering of phosphatidylinositol 4,5-bisphosphate (PIP) with proteins into what are known as "PIP rafts" is a critical component of intracellular signaling, yet little is known about PIP clusters at the atomic level. Using molecular dynamics simulations and network theory, this paper shows that Ca generates large clusters by linking PIP dimers already formed by doubly charged P4/P5 phosphates, while monovalent cations form smaller and less-stable clusters by adding PIP monomers preferentially via weaker interactions with P4/P5 (for Na) or with glycerol P1 (for K). Synergy arises between K and Ca because each ion forms linkages with different phosphates, thereby giving clusters more ways to grow. Read More

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Role of composted tannery solid waste and its autochthonous microbes in enhancing phytoextraction of toxic metals and stress abatement in sunflower.

Int J Phytoremediation 2022 May 23:1-11. Epub 2022 May 23.

Institute of Botany, Environmental Biotechnology Laboratory (F4), University of the Punjab, Lahore, Pakistan.

The excessive concentration of multiple heavy metals in the tannery solid waste (TSW) needs integrated process solutions for its decontamination. This study is aimed at deriving TSW compost and autochthonous microbe synergies for improving phytoextraction potential of sunflower. In-vessel composting of TSW was carried out by using fruit waste as an inoculum to achieve the optimized conditions. Read More

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Diversity of carbapenemase-producing in England as revealed by whole-genome sequencing of isolates referred to a national reference laboratory over a 30-month period.

J Med Microbiol 2022 May;71(5)

Antimicrobial Resistance and Healthcare Associated Infections (AMRHAI) Reference Unit, Reference Services Division, UK Health Security Agency, London, UK.

Increasing numbers of carbapenemase-producing (CPE), which can be challenging to treat, have been referred to the national reference laboratory in England since the early 2000s. Previous studies on CPE in the UK have focussed on localized outbreaks. We applied whole-genome sequencing (WGS) to isolates referred to the national reference laboratory over 30 months to inform our understanding of CPE epidemiology in England. Read More

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Computer-Aided Rational Construction of Mediated Bioelectrocatalysis with π-Conjugated (Hetero)cyclic Molecules: Toward Promoted Distant Electron Tunneling and Improved Biosensing.

Anal Chem 2022 May 23. Epub 2022 May 23.

Beijing National Laboratory for Molecular Science, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China.

Highly π-conjugated (hetero)cyclic molecules having delocalized orbitals and tunable charge mobilities are attractive redox relays for mediated bioelectrocatalysis in manifold applications. As rigid molecules, their dynamics within the soft but confined intraprotein space becomes the crucial determinant of the enzyme-mediator electron-tunneling efficiency. However, it is rarely investigated in designing the mediated interface with a particular biocatalyst (, oxidoreductase), which remains an empirical but try-and-error process. Read More

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Interplay of Electronic and Geometric Structure Tunes Organic Biradical Character in Bimetallic Tetrathiafulvalene Tetrathiolate Complexes.

J Phys Chem A 2022 May 23. Epub 2022 May 23.

Department of Chemistry and The James Franck Institute, The University of Chicago, Chicago, Illinois 60637, United States.

The synthesis and design of organic biradicals with tunable singlet-triplet gaps have become the subject of significant research interest, owing to their possible photochemical applications and use in the development of molecular switches and conductors. Recently, tetrathiafulvalene tetrathiolate (TTFtt) has been demonstrated to exhibit such organic biradical character in doubly ionized bimetallic complexes. In this article we use high-level calculations to interrogate the electronic structure of a series of TTFtt-bridged metal complexes, resolving the factors governing their biradical character and singlet-triplet gaps. Read More

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Species- and strain-level assessment using long-amplicons suggests donor's influence on gut microbial transference via fecal transplants in metabolic syndrome subjects.

Gut Microbes 2022 Jan-Dec;14(1):2078621

Microbial Ecology, Nutrition & Health Research Unit, Institute of Agrochemistry and Food Technology, Spanish National Research Council (IATA-CSIC), Paterna-Valencia, Spain.

Fecal microbiota transplantation (FMT) is currently used for treating infection and explored for other clinical applications in experimental trials. However, the effectiveness of this therapy could vary, and partly depend on the donor's bacterial species engraftment, whose evaluation is challenging because there are no cost-effective strategies for accurately tracking the microbe transference. In this regard, the precise identification of bacterial species inhabiting the human gut is essential to define their role in human health unambiguously. Read More

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Glioblastoma, an opportunity T cell trafficking could bring for the treatment.

Mol Biol Rep 2022 May 23. Epub 2022 May 23.

Department of Clinical Biochemistry, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.

Purpose: Infiltrating into the vital structure of the brain, located in the inaccessible anatomical region, and having molecular heterogeneity, glioblastoma (GBM) -with no doubt- is one of the deadliest cancers. Using the blood and brain barrier (BBB), GBM makes a shield to restrict the reach of chemotherapeutic agents to the tumor site and evolves a unique microenvironment to furnish all the essentials for cancer cells survival to conceal neoplastic cells from immunosurveillance.

Methods: 99 papers which met the criteria of eligibility were included in this review by consensus. Read More

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Non-invasive measurement of nuclear relative stiffness from quantitative analysis of microscopy data.

Eur Phys J E Soft Matter 2022 May 23;45(5):50. Epub 2022 May 23.

Dipartimento di Biotecnologie Mediche e Medicina Traslazionale, Universitá degli Studi di Milano, 20090, Segrate, Italy.

The connection between the properties of a cell tissue and those of the single constituent cells remains to be elucidated. At the purely mechanical level, the degree of rigidity of different cellular components, such as the nucleus and the cytoplasm, modulates the interplay between the cell inner processes and the external environment, while simultaneously mediating the mechanical interactions between neighboring cells. Being able to quantify the correlation between single-cell and tissue properties would improve our mechanobiological understanding of cell tissues. Read More

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Delineating the Bacteriome of Packaged and Loose Smokeless Tobacco Products Available in North India.

Appl Microbiol Biotechnol 2022 May 23. Epub 2022 May 23.

Division of Molecular Genetics and Biochemistry, Molecular Biology Group, ICMR-National Institute of Cancer Prevention and Research, Noida, Uttar Pradesh, India.

Smokeless tobacco product (STP) consumption is a significant public health threat across the globe. STPs are not only a storehouse of carcinogens and toxicants but also harbor microbes that aid in the conversion of tobacco alkaloids to carcinogenic tobacco-specific nitrosamines (TSNAs), thereby posing a further threat to the health of its consumers. The present study analyzed the bacterial diversity of popular dry and loose STPs by 16S rRNA gene sequencing. Read More

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Anti-epileptic Kunitz-like peptides discovered in the branching coral Acropora digitifera through transcriptomic analysis.

Arch Toxicol 2022 May 23. Epub 2022 May 23.

State Key Laboratory of Quality Research in Chinese Medicine and Institute of Chinese Medical Sciences, University of Macau, Macao, China.

Approximately 50 million people are suffering from epilepsy worldwide. Corals have been used for treating epilepsy in traditional Chinese medicine, but the mechanism of this treatment is unknown. In this study, we analyzed the transcriptome of the branching coral Acropora digitifera and obtained its Kyoto Encyclopedia of Genes and Genomes (KEGG), EuKaryotic Orthologous Groups (KOG) and Gene Ontology (GO) annotation. Read More

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Vibrationally excited intermolecular potential energy surfaces and the predicted near infrared overtone ( = 2 ← 0) spectra of a HO-Ne complex.

Phys Chem Chem Phys 2022 May 23. Epub 2022 May 23.

Institute of Theoretical Chemistry, College of Chemistry, Jilin University, 2519 Jiefang Road, Changchun 130023, P. R. China.

The intra- and inter-molecular potential energy surfaces (PESs) for the HO-Ne system that explicitly incorporate the intramolecular overtone state ( = 2) of HO are presented. The electronic structure computations have been carried out at the explicitly correlated coupled cluster theory [CCSD(T)-F12] level with an augmented correlation-consistent triple zeta basis set and an additional bond function. The vibrationally averaged three-dimensional intermolecular potentials for |00〉, |02〉, |02〉 and |11〉 are obtained analytically by fitting to the multi-dimensional Morse/Long-range potential function form. Read More

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Structural Basis for Binding of Potassium-Competitive Acid Blockers to the Gastric Proton Pump.

J Med Chem 2022 May 23. Epub 2022 May 23.

Graduate School of Pharmaceutical Sciences, Nagoya University, Nagoya, 464-8601, Japan.

As specific inhibitors of the gastric proton pump, responsible for gastric acidification, K-competitive acid blockers (P-CABs) have recently been utilized in the clinical treatment of gastric acid-related diseases in Asia. However, as these compounds have been developed based on phenotypic screening, their detailed binding poses are unknown. We show crystal and cryo-EM structures of the gastric proton pump in complex with four different P-CABs, tegoprazan, soraprazan, PF-03716556 and revaprazan, at resolutions reaching 2. Read More

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Surmounting the off-flavor challenge in plant-based foods.

Crit Rev Food Sci Nutr 2022 May 23:1-22. Epub 2022 May 23.

School of Agriculture and Food, The University of Melbourne, Parkville, Victoria, Australia.

Plant-based food products have been receiving an astronomical amount of attention recently, and their demand will most likely soar in the future. However, their unpleasant, intrinsic flavor and odor are the major obstacles limiting consumer's acceptance. These off-flavors are often described as "green," "grassy," "beany," "fatty" and "bitter. Read More

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The diet-microbiota axis: a key regulator of intestinal permeability in human health and disease.

Tissue Barriers 2022 May 21:2077069. Epub 2022 May 21.

Department of Biochemistry and Molecular Biology, University of Nebraska Medical Center, Omaha, Nebraska, USA.

The intestinal barrier orchestrates selective permeability to nutrients and metabolites while excluding noxious stimuli. Recent scientific advances establishing a causal role for the gut microbiota in human health outcomes have generated a resurgent interest toward intestinal permeability. Considering the well-established role of the gut barrier in protection against foreign antigens, there is mounting evidence for a causal link between gut permeability and the microbiome in regulating human health. Read More

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Light-Activated Vascular Anastomosis.

Surg Innov 2022 May 21:15533506221104382. Epub 2022 May 21.

Wellman Center for Photomedicine, Harvard Medical School, 2348Massachusetts General Hospital, Boston, MA, USA.

. There have been few advances in technique since vascular anastomosis was performed with silk suture on a curved needle in 1902. This technique results in disruption of the endothelium with exposed intraluminal suture, both of which may lead to thrombocyte aggregation, intimal hyperplasia, and vascular stenosis. Read More

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A chronic moderate methionine administration induced hyperhomocysteinemia associated with cardiovascular disease phenotype in the sand rat Psammomys obesus.

Folia Histochem Cytobiol 2022 May 23. Epub 2022 May 23.

Laboratory of Cellular and Molecular Biology, Biochemistry and Remodeling of the Extracellular Matrix, Faculty of Biological Sciences, Houari Boumediene University of Science and Technology (USTHB), Bab Ezzouar, El Alia, 16111, Algiers, Algeria.

Introduction: Cardiovascular diseases were defined as coronary artery, cerebrovascular, or peripheral arterial disease.Hyperhomocysteinemia (Hhcy) is an independent risk factor of cardiovascular diseases, including atherosclerosis. Our previous studies demonstrated the involvement of Hhcy in cardiovascular remodeling in the sand rat Psammomys obesus. Read More

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