3,316,855 results match your criteria molecular

Biosynthesis and extraction of high-value carotenoid from algae.

Front Biosci (Landmark Ed) 2021 May;26(6):171-190

Department of Botany, Mohanlal Sukhadia University, 313 001 Udaipur, Rajasthan, India.

Algae possess a considerable potential as bio-refinery for the scale-up production of high-value natural compounds like-carotenoids. Carotenoids are accessory pigments in the light-harvesting apparatus and also act as antioxidants and photo-protectors in green cells. They play important roles for humans, like-precursors of vitamin A, reduce the risk of some cancers, helps in the prevention of age-related diseases, cardiovascular diseases, improve skin health, and stimulates immunity. Read More

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Acetylsalicylic acid improves cognitive performance in sleep deprived adult Zebrafish () model.

Front Biosci (Landmark Ed) 2021 May;26(6):114-124

Department of Pharmacology, JSS College of Pharmacy, JSS Academy of Higher Education & Research, 570015 Mysuru, India.

Sleep deprivation (SD) is commonly associated with decreased attention, reduced responsiveness to external stimuli, and impaired locomotor and cognitive performances. Strong evidence indicates that SD disrupts neuro-immuno-endocrine system which is also linked to cognitive function. Recently Zebrafish have emerged as a powerful model sharing organizational and functional characteristics with other vertebrates, providing great translational relevance with rapid and reliable screening results. Read More

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Growth hormone treatment and bone mineral density in pediatric patients with Prader-Willi syndrome.

J Pediatr Endocrinol Metab 2021 Jun 23. Epub 2021 Jun 23.

Department of Pediatrics, Dokkyo Medical University Saitama Medical Center, Saitama, Japan.

Objectives: Previous reports indicate that growth hormone (GH) treatment for Prader-Willi syndrome (PWS) improves bone mineral density (BMD) only when initiated at a young age and not when initiated in adulthood. However, there are no data on BMD during long-term GH treatment of Japanese children and adolescents with PWS. Thus, this study aimed to investigate BMD changes among patients with PWS, who were undergoing GH treatment from childhood to adolescence. Read More

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Genome analyses and androgen quantification for an infant with 5α-reductase type 2 deficiency.

J Pediatr Endocrinol Metab 2021 Jun 23. Epub 2021 Jun 23.

Department of Molecular Endocrinology, National Research Institute for Child Health and Development, Tokyo, Japan.

Objectives: 5α-reductase type 2 deficiency due to biallelic variants is a common form of 46,XY disorders of sex development.

Case Presentation: A Chinese neonate presented with ambiguous genitalia. He carried a homozygous likely_pathogenic variant (c. Read More

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Retrospective evaluation of patients with X-linked adrenoleukodystrophy with a wide range of clinical presentations: a single center experience.

J Pediatr Endocrinol Metab 2021 Jun 23. Epub 2021 Jun 23.

Department of Pediatric Metabolism, Dr. Sami Ulus Maternity and Child Health Training and Research Hospital, Ankara, Turkey.

Objectives: X-linked adrenoleukodystrophy (X-ALD), is a peroxisomal inborn error of metabolism caused due to the loss of function variants of gene that leads to accumulation of very long chain fatty acids (VLCFAs) in several tissues including the neurological system. Childhood cerebral X-ALD (CCALD) is the most common and severe form of X-ALD, if left untreated. Allogenic hematopoietic stem cell transplantation (HSCT) is the only available therapy that halts neurological deterioration in CCALD. Read More

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Molecular and clinical findings of Turkish patients with hereditary fructose intolerance.

J Pediatr Endocrinol Metab 2021 Jun 23. Epub 2021 Jun 23.

Ankara Yıldırım Beyazıt University, Ankara City Hospital, Ankara, Turkey.

Objectives: Hereditary fructose intolerance (HFI) is an autosomal recessive disorder caused by a deficiency in aldolase B that can result in hypoglycemia, nausea, vomiting, abdominal pain, liver and kidney dysfunction, coma, and even death. This study aims to represent the clinical features and molecular genetic analysis data of the patients diagnosed with HFI in our study population.

Methods: The medical records of the 26 patients with HFI were evaluated retrospectively. Read More

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MAN1B1-CDG: novel patients and novel variant.

J Pediatr Endocrinol Metab 2021 Jun 23. Epub 2021 Jun 23.

Department of Pediatrics, Center for Metabolic Diseases, KU Leuven, Leuven, Belgium.

Objectives: Congenital disorders of glycosylation (CDGs) are a group of genetic disorders due to hypoglycosylation of proteins and lipids. A type I pattern is associated with defects in glycan assembly and transfer (CDG-I; cytosol; and endoplasmic reticulum defects), a type II pattern is seen in processing defects of the Golgi apparatus. MAN1B1-CDG is an autosomal recessive CDG-II due to mutations in the 1,2-mannosidase gene (MAN1B1), mainly characterized by psychomotor disability, facial dysmorphism, truncal obesity, and hypotonia. Read More

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Invasion history shapes host transcriptomic response to a body-snatching parasite.

Mol Ecol 2021 Jun 23. Epub 2021 Jun 23.

Department of Biology, Woods Hole Oceanographic Institution, Woods Hole, MA, USA.

By shuffling biogeographic distributions, biological invasions can both disrupt long-standing associations between hosts and parasites and establish new ones. This creates natural experiments with which to study the ecology and evolution of host-parasite interactions. In estuaries of the Gulf of Mexico, the white-fingered mud crab (Rhithropanopeus harrisii) is infected by a native parasitic barnacle Loxothylacus panopaei (Rhizocephala), which manipulates host physiology and behavior. Read More

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MRGPRX4 in Cholestatic Pruritus.

Semin Liver Dis 2021 Jun 23. Epub 2021 Jun 23.

Department of Neuroscience, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania.

Pruritus (itch) is a debilitating symptom in liver diseases with cholestasis, which severely affects patients' quality of life. Limited treatment options are available for cholestatic itch, largely due to the incomplete understanding of the underlying molecular mechanisms. Several factors have been proposed as pruritogens for cholestatic itch, such as bile acids, bilirubin, lysophosphatidic acid, and endogenous opioids. Read More

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The microscopic transition process from high-density to low-density amorphous state of SnI.

J Phys Condens Matter 2021 Jun 23. Epub 2021 Jun 23.

Physics, Ehime University, 2-5 Bunkyo-cho, Matsuyama, Ehime, 790-8577, JAPAN.

A molecular crystalline SnIundergoes pressure-induced solid-state amorphization via molecular dissociation to the high-density amorphous (HDA) state, which we call Am-I. In the present study, we examine the reverse transition process from Am-I to the low-density amorphous (LDA) state, called Am-II. We first measure the structure factor on decompression from 30 GPa down to 1. Read More

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SSMD: Semi-Supervised medical image detection with adaptive consistency and heterogeneous perturbation.

Med Image Anal 2021 Jun 3;72:102117. Epub 2021 Jun 3.

Department of Computer Science, The University of Hong Kong, Pokfulam, Hong Kong. Electronic address:

Semi-Supervised classification and segmentation methods have been widely investigated in medical image analysis. Both approaches can improve the performance of fully-supervised methods with additional unlabeled data. However, as a fundamental task, semi-supervised object detection has not gained enough attention in the field of medical image analysis. Read More

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Polystyrene microplastics induce microbial dysbiosis and dysfunction in surrounding seawater.

Environ Int 2021 Jun 20;156:106724. Epub 2021 Jun 20.

Center for Excellence in Regional Atmospheric Environment, Institute of Urban Environment, Chinese Academy of Sciences, 1799 Jimei Road, Xiamen 361021, China; Key Laboratory of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, 1799 Jimei Road, Xiamen 361021, China. Electronic address:

Microplastics are ubiquitously present in the environment, accumulate in aquaculture water, and cause toxicological effects on aquatic organisms. Besides, microplastics provide ecological niches for microorganisms in aquatic environments. However, the effects of microplastics on microbial balance and function in surrounding water are still unclear, especially for aquaculture water. Read More

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Prevalence, healthcare resource utilization and mortality of Lennox-Gastaut syndrome: retrospective linkage cohort study.

Seizure 2021 Jun 9;91:159-166. Epub 2021 Jun 9.

Evidence Synthesis, Modeling, Communications, Evidera, The Ark, 201 Talgarth Road, London W6 8BJ, UK. Electronic address:

Purpose: To retrospectively investigate the prevalence, demography, antiseizure medication (ASM) usage, healthcare resource utilization (HCRU), and mortality of patients with Lennox-Gastaut syndrome (LGS) in primary and secondary care in the UK.

Methods: Patients with confirmed LGS were anonymously identified from the UK Clinical Practice Research Datalink (CPRD) GOLD database (01/01/1987-31/10/2018) using the LGS Read Code (F250500). Probable LGS was identified using the International Classification of Diseases-10/Read Code for epilepsy (Hospital Episode Statistics [HES]/CPRD) plus rufinamide prescription. Read More

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Comparative analysis of hippocampal transcriptional features between major depressive disorder patients and animal models.

J Affect Disord 2021 Jun 11;293:19-28. Epub 2021 Jun 11.

NHC Key Laboratory of Diagnosis and Treatment on Brain Functional Diseases, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China; Department of Neurology, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China. Electronic address:

Background: Major depressive disorder (MDD) is a psychiatric disorder caused by various etiologies. Chronic stress models are used to simulate the heterogeneous pathogenic processes of depression. However, few studies have compared transcriptional features between stress models and MDD patients. Read More

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The Cd-induced morphological and photosynthetic disruption is related to the reduced Fe status and increased oxidative injuries in sugar beet.

Plant Physiol Biochem 2021 Jun 17;166:448-458. Epub 2021 Jun 17.

Molecular Plant Physiology Laboratory, Department of Botany, University of Rajshahi, Rajshahi, 6205, Bangladesh. Electronic address:

Cadmium (Cd) toxicity is a form of soil contamination that causes losses in plant growth and yield. Understanding the effects of Cd-induced changes in physiological and cellular processes will help scientists develop better scientific strategies for sugar beet plant improvement. Cd toxicity triggered a substantial decrease in morphological parameters and total soluble protein in sugar beets, as well as membrane damage and cell death. Read More

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New pyrimidine and pyrazole-based compounds as potential EGFR inhibitors: Synthesis, anticancer, antimicrobial evaluation and computational studies.

Bioorg Chem 2021 Jun 10;114:105078. Epub 2021 Jun 10.

Pharmaceutical Medicinal Chemistry and Drug Design Department, Faculty of Pharmacy (Girls), Al-Azhar University, Cairo 11754, Egypt.

This study was focused on the synthesis of new pyrimidines 4a,b, 5a,b and pyrazoles 6a, b as ATP mimicking tyrosine kinase inhibitors of the epidermal growth factor receptor (EGFR). The new compounds were assessed as cytotoxic candidates against human breast cancer cells (MCF-7) and hepatocellular carcinoma cells (HepG-2). All the new compounds appeared as more potent cytotoxic agents than erlotinib, while only compound 4a exhibited more potency than 5-flourouracil and 4b analogue was equipotent to it. Read More

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Are other tick-borne infections overlooked in patients investigated for Lyme neuroborreliosis? A large retrospective study from South-eastern Sweden.

Ticks Tick Borne Dis 2021 Jun 4;12(5):101759. Epub 2021 Jun 4.

Department of Biomedical and Clinical Sciences, Division of Inflammation and Infection, Linköping University, Linköping, Sweden; Department of Infectious Diseases, Region Östergötland County, Linköping/Norrköping, Sweden.

In Europe, the hard tick Ixodes ricinus is considered the most important vector of human zoonotic diseases. Human pathogenic agents spread by I. ricinus in Sweden include Borrelia burgdorferi sensu lato (s. Read More

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Si113-prodrugs selectively activated by plasmin against hepatocellular and ovarian carcinoma.

Eur J Med Chem 2021 Jun 17;223:113653. Epub 2021 Jun 17.

Dipartimento di Biotecnologie, Chimica e Farmacia, Università degli Studi di Siena, Via Aldo Moro 2, 53100, Siena, Italy; Lead Discovery Siena S.r.l., Via Vittorio Alfieri 31, 53019, Castelnuovo Berardenga, Siena, Italy; Sbarro Institute for Cancer Research and Molecular Medicine, Center for Biotechnology, College of Science and Technology Temple University, BioLife Science Building, Suite 333, 1900 North 12th Street, Philadelphia, PA, 19122, United States.

Si113, a pyrazolo[3,4-d]pyrimidine derivative, gained more attention as an anticancer agent due to its potent anticancer activity on both in vitro and in vivo hepatocellular carcinomas (HCC) and ovarian carcinoma models. But the drawback is the low water solubility which prevents its further development. In this context, we successfully overcame this limitation by synthesizing two novel prodrugs introducing the amino acid sequence D-Ala-Leu-Lys (TP). Read More

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Repairing damaged lungs using regenerative therapy.

Curr Opin Pharmacol 2021 Jun 20;59:85-94. Epub 2021 Jun 20.

Department of Pulmonology, Leiden University Medical Center, Leiden, the Netherlands. Electronic address:

There is an urgent need for better treatment of lung diseases that are a major cause of morbidity and mortality worldwide. This urgency is illustrated by the current COVID-19 health crisis. Moderate-to-extensive lung injury characterizes several lung diseases, and not only therapies that reduce such lung injury are needed but also those that regenerate lung tissue and repair existing lung injury. Read More

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A rotational study of the 1:1 adduct of ethanol and 1,4-dioxane.

Spectrochim Acta A Mol Biomol Spectrosc 2021 Jun 16;261:120086. Epub 2021 Jun 16.

Dipartimento di Chimica "Giacomo Ciamician" dell'Università, Via Selmi 2, I-40126 Bologna, Italy.

The pure rotational spectra of the 1:1 ethanol - 1,4-dioxane complex and its OD mono-deuterated species have been measured using pulsed-jet Fourier transform microwave spectroscopy. Conformational predictions for the plausible isomers of ethanol - 1,4-dioxane have been carried out considering the spatial orientation of gauche/trans ethanol with respect to the chair/boat and twisted conformations of 1,4-dioxane. Using Helium for the supersonic expansion, the microwave spectrum has been observed for the most stable structure. Read More

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Structural insights into understudied human cytochrome P450 enzymes.

Drug Discov Today 2021 Jun 20. Epub 2021 Jun 20.

Pharmaceutical and Medicinal Chemistry (Computer-Aided Drug Design), Institute of Pharmacy, Freie Universität Berlin, 14195 Berlin, Germany. Electronic address:

Human cytochrome P450 (CYP) enzymes are widely known for their pivotal role in the metabolism of drugs and other xenobiotics as well as of endogenous chemicals. In addition, CYPs are involved in numerous pathophysiological pathways and, hence, are therapeutically relevant. Remarkably, a portion of promising CYP targets is still understudied and, as a consequence, untargeted, despite their huge therapeutic potential. Read More

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A narrative review of clinical studies of herbal treatment of difficult to manage asthma.

Complement Ther Clin Pract 2021 Jun 16;44:101433. Epub 2021 Jun 16.

School of Life Sciences, Faculty of Science, University of Technology Sydney, Sydney, NSW, 2007, Australia; Respiratory Cellular and Molecular Biology, Woolcock Institute of Medical Research, The University of Sydney, Sydney, NSW, 2037, Australia. Electronic address:

Asthma can be complex and difficult to manage. Patients often seek alternative treatment options, including Traditional Chinese Medicine (TCM). The paradigms that inform TCM treatments include a philosophy focusing on modifying the whole body status ('bian zheng') to treat the lungs. Read More

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Splenic Infarction with Aortic Thrombosis in COVID-19.

Am J Med Sci 2021 Jun 20. Epub 2021 Jun 20.

Intensive Care Unit, Emílio Ribas Institute of Infectious Diseases, São Paulo, Brazil. Electronic address:

Coronavirus disease 2019 (COVID-19) has been associated with an increased risk of venous and arterial thrombotic disease. Although pulmonary embolism has been the most common thrombotic complication, there have been recent reports of COVID-19-associated large-vessel ischemic stroke, acute upper- and lower-limb ischemia, as well as infarctions of the abdominal viscera, including renal, splenic, and small bowel infarctions. Here, we describe a case of splenic infarction (SI) associated with aortic thrombosis, which evolved despite the prophylactic use of low-molecular-weight heparin (LMWH), in a 60-year-old female patient with COVID-19. Read More

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Arecanut-induced fibrosis display dual phases of reorganising glycans and amides in skin extracellular matrix.

Int J Biol Macromol 2021 Jun 20. Epub 2021 Jun 20.

School of Medical Science and Technology, Indian Institute of Technology Kharagpur, West Bengal 721302, India.

The habit of chewing arecanut leads to fibrosis in the oral tissues, which can lead to cancer. Despite high mortality, fibrosis has limited clinical success owing to organ-specific variations, genetic predispositions, and slow progression. Fibrosis is a progressive condition that is unresponsive to medications in the severe phase. Read More

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Comparison of two assays to detect IgG antibodies to the receptor binding domain of the SARS‑CoV‑2 as a surrogate marker for assessing neutralizing antibodies in COVID-19 patients.

Int J Infect Dis 2021 Jun 20. Epub 2021 Jun 20.

AICBU, Department of Immunology and Molecular Medicine, Faculty of Medical Sciences, University of Sri Jayewardenapura, Sri Lanka; MRC Human Immunology Unit, MRC Weatherall Institute of Molecular Medicine, University of Oxford, Oxford, United Kingdom. Electronic address:

Background: Neutralizing antibodies (NAbs) are important for protection against COVID-19 re-infection. We compared two assays that are correlated with NAbs, the haemagglutination test (HAT) and surrogate virus neutralization test (sVNT).

Methods: The specificity of the HAT was compared with the sVNT and the sensitivity and persistence of antibodies in patients with varying severity of illness was assessed in cohort of 71 patients at 4 to 6 weeks and 13-16 weeks. Read More

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Screen of anti-migraine active compounds from Duijinsan by spectrum-effect relationship analysis and molecular docking.

J Ethnopharmacol 2021 Jun 20:114352. Epub 2021 Jun 20.

Tang Center of Herbal Medicine Research and Department of Anesthesia & Critical Care, University of Chicago, Chicago, IL, 60637, USA. Electronic address:

Ethnopharmacological Relevance: Duijinsan (DJS) is a famous Chinese medicine prescription composed of Radix scutellariae (RS) and Rhei Radix (RRR), which has been mainly used for treating migraine.

Aim Of The Study: This study aimed to uncover the anti-migraine active compounds from DJS and preliminary predicted the pharmacological mechanism by evaluating the spectrum-effect relationship between high-performance liquid chromatography (HPLC) fingerprints and anti-migraine effects of Duijinsan (DJS) extract combined with molecular docking.

Materials And Methods: HPLC and LC-MS were applied for chemical analyses of DJS extracts in different proportions. Read More

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UM-6 induces autophagy and apoptosis via the Hippo-YAP signaling pathway in cervical cancer.

Cancer Lett 2021 Jun 20. Epub 2021 Jun 20.

Department of Obstetrics and Gynecology, The Second Hospital, Jilin University, 218 Zi Qiang Street, Changchun, Jilin, 130000, PR China. Electronic address:

Melittin's non-specific cytotoxicity and hemolytic activity restrict its clinical use, but polypeptide modification is thoμght to be highly selective and well-tolerated. Here, we synthesized a novel antineoplastic peptide UM-6 based on melittin and explored the mechanism related to its anti-proliferation and metastasis on cervical cancer (CC). In the present study, we demonstrated that UM-6 inhibits viability of CC cell lines Caski and Hela in vitro by inducing apoptosis and autophagy with low toxicity to normal epithelial cells. Read More

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Activity-based, bioorthogonal imaging of phospholipase D reveals spatiotemporal dynamics of GPCR-Gq signaling.

Cell Chem Biol 2021 Jun 9. Epub 2021 Jun 9.

Department of Chemistry and Chemical Biology and Weill Institute for Cell and Molecular Biology, Cornell University, Ithaca, NY, USA. Electronic address:

Canonically, G-protein-coupled receptor (GPCR) signaling is transient and confined to the plasma membrane (PM). Deviating from this paradigm, the parathyroid hormone receptor (PTHR1) stimulates sustained G signaling at endosomes. In addition to G, PTHR1 activates G signaling; yet, in contrast to the PTHR1-G pathway, the spatiotemporal dynamics of the G branch of PTHR1 signaling and its relationship to G signaling remain largely ill defined. Read More

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Molecular recognition of structurally disordered Pro/Ala-rich sequences (PAS) by antibodies involves an Ala residue at the hot spot of the epitope.

J Mol Biol 2021 Jun 20:167113. Epub 2021 Jun 20.

Lehrstuhl für Biologische Chemie Technische Universität München Emil-Erlenmeyer-Forum 5 85354 Freising Germany; XL-protein GmbH Lise-Meitner-Strasse 30 85354 Freising Germany.

Pro/Ala-rich sequences (PAS) are polypeptides that were developed as a biological alternative to poly-ethylene glycol (PEG) to generate biopharmaceuticals with extended plasma half-life. Like PEG PAS polypeptides are conformationally disordered and show high solubility in water. Devoid of any charged or prominent hydrophobic side chains these biosynthetic polymers represent an extreme case of intrinsically disordered proteins. Read More

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A novel N-terminal region to chromodomain in CHD7 is required for the efficient remodeling activity.

J Mol Biol 2021 Jun 20:167114. Epub 2021 Jun 20.

Department of Biological Sciences, Korea Advanced Institute of Science and Technology (KAIST), KAIST Institute of BioCentury, Daejeon 34141, Korea. Electronic address:

Chromodomain-Helicase DNA binding protein 7 (CHD7) is an ATP dependent chromatin remodeler involved in maintaining open chromatin structure. Mutations of CHD7 gene causes multiple developmental disorders, notably CHARGE syndrome. However, there is not much known about the molecular mechanism by which CHD7 remodels nucleosomes. Read More

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