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Structure of CRL2Lrr1, the E3 ubiquitin ligase that promotes DNA replication termination in vertebrates.

Nucleic Acids Res 2021 Dec 1. Epub 2021 Dec 1.

Department of Biological Chemistry and Molecular Pharmacology, Blavatnik Institute, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA.

When vertebrate replisomes from neighboring origins converge, the Mcm7 subunit of the replicative helicase, CMG, is ubiquitylated by the E3 ubiquitin ligase, CRL2Lrr1. Polyubiquitylated CMG is then disassembled by the p97 ATPase, leading to replication termination. To avoid premature replisome disassembly, CRL2Lrr1 is only recruited to CMGs after they converge, but the underlying mechanism is unclear. Read More

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December 2021

CeLINC, a fluorescence-based protein-protein interaction assay in Caenorhabditiselegans.

Genetics 2021 Oct 2. Epub 2021 Oct 2.

Division of Developmental Biology, Department of Biology, Faculty of Science, Institute of Biodynamics and Biocomplexity, Utrecht University, 3584 CH Utrecht, the Netherlands.

Interactions among proteins are fundamental for life and determining whether two particular proteins physically interact can be essential for fully understanding a protein's function. We present Caenorhabditiselegans light-induced coclustering (CeLINC), an optical binary protein-protein interaction assay to determine whether two proteins interact in vivo. Based on CRY2/CIB1 light-dependent oligomerization, CeLINC can rapidly and unambiguously identify protein-protein interactions between pairs of fluorescently tagged proteins. Read More

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October 2021

Natural Flt3Lg-Based Chimeric Antigen Receptor (Flt3-CAR) T Cells Successfully Target Flt3 on AML Cell Lines.

Vaccines (Basel) 2021 Oct 25;9(11). Epub 2021 Oct 25.

Dmitry Rogachev National Medical Research Center of Pediatric Hematology, Oncology and Immunology, 117997 Moscow, Russia.

Relapsed/refractory acute myeloid leukemia (AML) cannot be cured with chemotherapy alone, as the blasts survive the treatment. Chimeric antigen receptor (CAR) approaches for AML are being actively developed. CARs promote immune reactions through recognition of the target molecular epitopes at the surface of cancer cells. Read More

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October 2021

Identification of Prognostic Gene Biomarkers in Non-Small Cell Lung Cancer Progression by Integrated Bioinformatics Analysis.

Biology (Basel) 2021 Nov 18;10(11). Epub 2021 Nov 18.

School of Applied Sciences, University of Brighton, Lewes Road, Brighton BN2 4GJ, UK.

The progression of non-small cell lung cancer (NSCLC) is linked to epithelial-mesenchymal transition (EMT), a biologic process that enables tumor cells to acquire a migratory phenotype and resistance to chemo- and immunotherapies. Discovery of novel biomarkers in NSCLC progression is essential for improved prognosis and pharmacological interventions. In the current study, we performed an integrated bioinformatics analysis on gene expression datasets of TGF-β-induced EMT in NSCLC cells to identify novel gene biomarkers and elucidate their regulation in NSCLC progression. Read More

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November 2021

A visual-degradation-inspired model with HSV color-encoding for contour detection.

J Neurosci Methods 2021 Nov 23:109423. Epub 2021 Nov 23.

Institute for Cognitive Neurodynamics, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China. Electronic address:

Background: Given energy metabolism, visual information degradation plays an essential role in the retina- lateral geniculate nucleus (LGN)-primary visual cortex (V1)-secondary visual cortex (V2) pathway, and is a pivotal issue for visual information processing. Degradation helps the visual nervous system conserve brain energy and efficiently perceive the real world even though a small fraction of visual information reaches the early visual areas. The coding of contour features (edge and corner) is achieved in the retina-LGN-V1-V2 pathway. Read More

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November 2021

Modeling and experimental analysis of battery charge controllers for comparing three off-grid photovoltaic power plants.

Heliyon 2021 Nov 8;7(11):e08331. Epub 2021 Nov 8.

Fort Hare Institute of Technology, University of Fort Hare, Alice, 5700, South Africa.

The study of battery charge algorithm as a sole power storage agent in off-grid systems is essential. The battery charge algorithm has various methods, and the battery in these methods relies on the quantity of charges. Hence, a charge controller is used to safeguard and regulate battery charge and discharge for off-grid photovoltaic (PV) systems. Read More

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November 2021

Drr4covid: Learning Automated COVID-19 Infection Segmentation From Digitally Reconstructed Radiographs.

IEEE Access 2020 16;8:207736-207757. Epub 2020 Nov 16.

School of Life Science, Beijing Institute of Technology Beijing 100081 China.

Automated infection measurement and COVID-19 diagnosis based on Chest X-ray (CXR) imaging is important for faster examination, where infection segmentation is an essential step for assessment and quantification. However, due to the heterogeneity of X-ray imaging and the difficulty of annotating infected regions precisely, learning automated infection segmentation on CXRs remains a challenging task. We propose a novel approach, called DRR4Covid, to learn COVID-19 infection segmentation on CXRs from digitally reconstructed radiographs (DRRs). Read More

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November 2020

Graphical user interface for the haplotype frequency estimation software Hapl-o-Mat.

Hum Immunol 2021 Nov 16. Epub 2021 Nov 16.

DKMS, Tübingen, Germany.

Population-specific human leukocyte antigen (HLA) haplotype frequencies are an essential basis of advanced algorithms for donor selection in unrelated hematopoietic stem cell transplantation. In 2016, we introduced Hapl-o-Mat, a versatile tool for haplotype frequency estimation based on an expectation-maximization algorithm (https://github.com/DKMS/hapl-o-Mat). Read More

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November 2021

Sensor histidine kinases mediate ABA and osmostress signaling in the moss Physcomitrium patens.

Curr Biol 2021 Nov 12. Epub 2021 Nov 12.

Department of Bioscience, Tokyo University of Agriculture, Tokyo 156-8502, Japan. Electronic address:

To survive fluctuating water availability on land, terrestrial plants must be able to sense water stresses, such as drought and flooding. The plant hormone abscisic acid (ABA) and plant-specific SNF1-related protein kinase 2 (SnRK2) play key roles in plant osmostress responses. We recently reported that, in the moss Physcomitrium patens, ABA and osmostress-dependent SnRK2 activation requires phosphorylation by an upstream RAF-like kinase (ARK). Read More

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November 2021

Design of a Vibration Damping Robot and Force Evaluation In Intraoperative Robotic Assisted Femoral Shaft Repair Using a Modified Soft Damper.

Int J Med Robot 2021 Nov 18:e2349. Epub 2021 Nov 18.

School of Mechanical Engineering, Southeast University, Nanjing, P.R China.

Closed intramedullary nailing fixation is a method for treating fractured femurs with minimal invasiveness. However, this method lacks safety and precision. To avert prevailing problems such as extended cracks in the already broken bone, the design of a vibration damping robot and force evaluation system is essential. Read More

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November 2021

Formation of nuclear condensates by the Mediator complex subunit Med15 in mammalian cells.

BMC Biol 2021 11 17;19(1):245. Epub 2021 Nov 17.

Molecular Cancer Research Center, School of Medicine, Shenzhen Campus of Sun Yat-sen University, Sun Yat-sen University, Shenzhen, China.

Background: The Mediator complex is an evolutionarily conserved multi-subunit protein complex that plays major roles in transcriptional activation and is essential for cell growth, proliferation, and differentiation. Recent studies revealed that some Mediator subunits formed nuclear condensates that may facilitate enhancer-promoter interactions and gene activation. The assembly, regulation, and functions of these nuclear condensates remain to be further understood. Read More

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November 2021

Salmonella Genomic Island 1 requires a self-encoded small RNA for mobilization.

Mol Microbiol 2021 Nov 16. Epub 2021 Nov 16.

Institute of Genetics and Biotechnology, Hungarian University of Agriculture and Life Sciences, Gödöllő, Hungary.

The SGI1-family elements that are specifically mobilized by the IncA- and IncC-family plasmids are important vehicles of antibiotic resistance among enteric bacteria. Although SGI1 exploits many plasmid-derived conjugation and regulatory functions, the basic mobilization module of the island is unrelated to that of IncC plasmids. This module contains the oriT and encodes the mobilization proteins MpsA and MpsB, which belong to the tyrosine recombinases and not to relaxases. Read More

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November 2021

Zipper head mechanism of telomere synthesis by human telomerase.

Cell Res 2021 Nov 15. Epub 2021 Nov 15.

Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Telomerase, a multi-subunit ribonucleoprotein complex, is a unique reverse transcriptase that catalyzes the processive addition of a repeat sequence to extend the telomere end using a short fragment of its own RNA component as the template. Despite recent structural characterizations of human and Tetrahymena telomerase, it is still a mystery how telomerase repeatedly uses its RNA template to synthesize telomeric DNA. Here, we report the cryo-EM structure of human telomerase holoenzyme bound with telomeric DNA at resolutions of 3. Read More

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November 2021

Development of an IoT-Based Sleep Apnea Monitoring System for Healthcare Applications.

Comput Math Methods Med 2021 3;2021:7152576. Epub 2021 Nov 3.

Department of Electrical and Computer Engineering, North South University, Bashundhara, Dhaka 1229, Bangladesh.

Sleep is an essential and vital element of a person's life and health that helps to refresh and recharge the mind and body of a person. The quality of sleep is very important in every person's lifestyle, removing various diseases. Bad sleep is a big problem for a lot of people for a very long time. Read More

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November 2021

The IbBBX24-IbTOE3-IbPRX17 module enhances abiotic stress tolerance by scavenging reactive oxygen species in sweet potato.

New Phytol 2021 Nov 13. Epub 2021 Nov 13.

Key Laboratory of Sweet Potato Biology and Biotechnology, Ministry of Agriculture and Rural Affairs/Beijing Key Laboratory of Crop Genetic Improvement/Laboratory of Crop Heterosis & Utilization and Joint Laboratory for International Cooperation in Crop Molecular Breeding, College of Agronomy & Biotechnology, Ministry of Education, China Agricultural University, Beijing, 100193, China.

Soil salinity and drought limit sweet potato yield. Scavenging of reactive oxygen species (ROS) by peroxidases (PRXs) is essential during plant stress responses, but how PRX expression is regulated under abiotic stress is not well understood. Here, we report that the B-box (BBX) family transcription factor IbBBX24 activates the expression of the class III peroxidase gene IbPRX17 by binding to its promoter. Read More

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November 2021

Annual prevalence of unmet healthcare need in Thailand: evidence from national household surveys between 2011 and 2019.

Int J Equity Health 2021 Nov 12;20(1):244. Epub 2021 Nov 12.

International Health Policy Program, Ministry of Public Health, Nonthaburi, Thailand.

Background: Extending Universal Health Coverage (UHC) requires identifying and addressing unmet healthcare need and its causes to improve access to essential health services. Unmet need is a useful monitoring indicator to verify if low incidence of catastrophic health spending is not a result of foregone services due to unmet needs. This study assesses the trend, between 2011 and 2019, of prevalence and reasons of unmet healthcare need and identifies population groups who had unmet needs. Read More

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November 2021

Gene expression changes related to bone mineralization, blood pressure and lipid metabolism in mouse kidneys after space travel.

Kidney Int 2021 Nov 9. Epub 2021 Nov 9.

Department of Integrative Genomics, Tohoku Medical Megabank Organization, Tohoku University, Sendai, Japan; Department of Medical Biochemistry, Tohoku University Graduate School of Medicine, Sendai, Japan.

Space travel burdens health by imposing considerable environmental stress associated with radioactivity and microgravity. In particular, gravity change predominantly impacts blood pressure and bone homeostasis, both of which are controlled mainly by the kidneys. Nuclear factor erythroid-2-related transcription factor 2 (Nrf2) plays essential roles in protecting the kidneys from various environmental stresses and injuries. Read More

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November 2021

Experimental study of the potential for thermal energy recovery with thermoelectric devices in low displacement diesel engines.

Heliyon 2021 Nov 28;7(11):e08273. Epub 2021 Oct 28.

KAI Research Unit, Department of Mechanical Engineering, Universidad del Atlántico, Carrera 30 Número 8-49, Puerto Colombia, Barranquilla, Colombia.

Improving the thermal efficiency of internal combustion engines is essential to reduce the operating costs and complaints with the increasing environmental requirements. Thermoelectric generators came up as an opportunity to reuse part of the heat loss with the exhausts. This paper evaluates the performance of a thermoelectric generator to improve the efficiency of a stationary diesel engine under different rotational speeds and torques. Read More

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November 2021

Stiffer Spleen Predicts Higher Bone Marrow Fibrosis and Higher Allele Burden in Patients With Myeloproliferative Neoplasms.

Front Oncol 2021 26;11:777730. Epub 2021 Oct 26.

Division of Internal Medicine, Department of Translational Medicine, Università del Piemonte Orientale and Azienda Ospedaliero-Universitaria Maggiore della Carità, Novara, Italy.

A total of 63 myeloproliferative neoplasms [MPN; 9 polycythemia vera (PV), 32 essential thrombocythemia (ET), and 22 myelofibrosis (MF)] underwent spleen stiffness (SS) measurement by vibration-controlled transient elastography equipped with a novel spleen-dedicated module. Higher SS values significantly correlated with grade 2-3 bone marrow (BM) fibrosis (=0.035), with hemoglobin level <10 g/dl (=0. Read More

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October 2021

Multiple instance convolutional neural network with modality-based attention and contextual multi-instance learning pooling layer for effective differentiation between borderline and malignant epithelial ovarian tumors.

Artif Intell Med 2021 11 12;121:102194. Epub 2021 Oct 12.

Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou, Jiangsu 215163, China; Jinan Guoke Medical Engineering and Technology Development Co., Ltd., Jinan, Shandong 250109, China; Department of Radiology, Shanxi Province Cancer Hospital, Shanxi Medical University, Taiyuan, Shanxi 030013, China. Electronic address:

Malignant epithelial ovarian tumors (MEOTs) are the most lethal gynecologic malignancies, accounting for 90% of ovarian cancer cases. By contrast, borderline epithelial ovarian tumors (BEOTs) have low malignant potential and are generally associated with a good prognosis. Accurate preoperative differentiation between BEOTs and MEOTs is crucial for determining the appropriate surgical strategies and improving the postoperative quality of life. Read More

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November 2021

Identification and Validation of IFI44 as Key Biomarker in Lupus Nephritis.

Front Med (Lausanne) 2021 25;8:762848. Epub 2021 Oct 25.

Kidney Disease Center, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China.

Lupus nephritis (LN) is a common and severe organ manifestation of systemic lupus erythematosus (SLE) and is a major cause of SLE related deaths. Early diagnosis is essential to improve the prognosis of patients with LN. To screen the potential biomarkers associated with LN, we downloaded the gene expression profile of GSE99967 from the Gene Expression Omnibus (GEO) database. Read More

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October 2021

HFRU-Net: High-Level Feature Fusion and Recalibration UNet for Automatic Liver and Tumor Segmentation in CT Images.

Comput Methods Programs Biomed 2021 Oct 28;213:106501. Epub 2021 Oct 28.

Department of Electronics and Telecommunication Engineering, Shri Guru Gobind Singhji Institute of Engineering and Technology, Nanded, Maharashtra, India.

Automatic liver and tumor segmentation are essential steps to take decisive action in hepatic disease detection, deciding therapeutic planning, and post-treatment assessment. The computed tomography (CT) scan has become the choice of medical experts to diagnose hepatic anomalies. However, due to advancements in CT image acquisition protocol, CT scan data is growing and manual delineation of the liver and tumor from the CT volume becomes cumbersome and tedious for medical experts. Read More

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October 2021

Prediction of transcription factor binding sites with an attention augmented convolutional neural network.

IEEE/ACM Trans Comput Biol Bioinform 2021 Nov 9;PP. Epub 2021 Nov 9.

Identification of transcription factor binding sites (TFBSs) is essential for revealing the rules of protein-DNA binding. Although some computational methods have been presented to predict TFBSs using epigenomic and sequence features, most of them ignore the common features among cross-cell types. It is still unclear to what extent the common features could help for this task. Read More

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November 2021

MIFAD-Net: Multi-Layer Interactive Feature Fusion Network With Angular Distance Loss for Face Emotion Recognition.

Front Psychol 2021 22;12:762795. Epub 2021 Oct 22.

College of Sports Engineering and Information Technology, Wuhan Sports University, Wuhan, China.

Understanding human emotions and psychology is a critical step toward realizing artificial intelligence, and correct recognition of facial expressions is essential for judging emotions. However, the differences caused by changes in facial expression are very subtle, and different expression features are less distinguishable, making it difficult for computers to recognize human facial emotions accurately. Therefore, this paper proposes a novel multi-layer interactive feature fusion network model with angular distance loss. Read More

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October 2021

Tuning structural isomers of phenylenediammonium to afford efficient and stable perovskite solar cells and modules.

Nat Commun 2021 Nov 4;12(1):6394. Epub 2021 Nov 4.

Institute of Chemical Sciences and Engineering, EPFL VALAIS, Sion, 1951, Switzerland.

Organic halide salt passivation is considered to be an essential strategy to reduce defects in state-of-the-art perovskite solar cells (PSCs). This strategy, however, suffers from the inevitable formation of in-plane favored two-dimensional (2D) perovskite layers with impaired charge transport, especially under thermal conditions, impeding photovoltaic performance and device scale-up. To overcome this limitation, we studied the energy barrier of 2D perovskite formation from ortho-, meta- and para-isomers of (phenylene)di(ethylammonium) iodide (PDEAI) that were designed for tailored defect passivation. Read More

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November 2021

Identification of hub genes and molecular subtypes in COVID-19 based on WGCNA.

Eur Rev Med Pharmacol Sci 2021 Oct;25(20):6411-6424

Department of Gastrointestinal Surgery, Henan Provincial People's Hospital, Zhengzhou, Henan, China.

Objective: The heterogeneity of clinical manifestations and mortality rates in Coronavirus disease 2019 (COVID-19) patients may be related to the existence of molecular subtypes in COVID-19. To improve current management, it is essential to find the hub genes and pathways associated with different COVID-19 subtypes.

Materials And Methods: The whole-genome sequencing information (GSE156063, GSE163151) of nasopharyngeal swabs from normal subjects and COVID-19 patients were downloaded from the Gene Expression Omnibus (GEO) database. Read More

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October 2021

Multilevel Attention Networks and Policy Reinforcement Learning for Image Caption Generation.

Big Data 2021 Nov 2. Epub 2021 Nov 2.

School of Computer Science and Engineering, Beihang University, Beijing, China.

The analysis of large-scale multimodal data has become very popular recently. Image captioning, whose goal is to describe the content of image with natural language automatically, is an essential and challenging task in artificial intelligence. Commonly, most existing image caption methods utilize the mixture of Convolutional Neural Network and Recurrent Neural Network framework. Read More

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November 2021

Oxidized Product Profiles of AA9 Lytic Polysaccharide Monooxygenases Depend on the Type of Cellulose.

ACS Sustain Chem Eng 2021 Oct 13;9(42):14124-14133. Epub 2021 Oct 13.

Laboratory of Food Chemistry, Wageningen University & Research, Bornse Weilanden 9, 6708 WG Wageningen, The Netherlands.

Lytic polysaccharide monooxygenases (LPMOs) are essential for enzymatic conversion of lignocellulose-rich biomass in the context of biofuels and platform chemicals production. Considerable insight into the mode of action of LPMOs has been obtained, but research on the cellulose specificity of these enzymes is still limited. Hence, we studied the product profiles of four fungal Auxiliary Activity family 9 (AA9) LPMOs during their oxidative cleavage of three types of cellulose: bacterial cellulose (BC), Avicel PH-101 (AVI), and regenerated amorphous cellulose (RAC). Read More

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October 2021

Optimized-Unet: Novel Algorithm for Parapapillary Atrophy Segmentation.

Front Neurosci 2021 13;15:758887. Epub 2021 Oct 13.

The Affiliated Eye Hospital of Nanjing Medical University, Nanjing, China.

In recent years, an increasing number of people have myopia in China, especially the younger generation. Common myopia may develop into high myopia. High myopia causes visual impairment and blindness. Read More

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October 2021

Micro telecom computing architecture.4-based beam position monitor electronics design for storage ring of Hefei advanced light facility.

Rev Sci Instrum 2021 Oct;92(10):104711

National Synchrotron Radiation Laboratory, University of Science and Technology of China, Hefei, Anhui 230026, China.

The Hefei Advanced Light Facility (HALF) is a fourth-generation vacuum ultraviolet and x-ray diffraction limit synchrotron radiation (DLSR) light source now under preliminary research. To achieve ultralow beam emittance and small beam size, the orbit of the beam in the DLSR storage ring should meet the stability requirement at submicrometer scale. The beam position monitor (BPM) electronics measures the orbit and is hence an essential part of the beam orbit control system. Read More

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October 2021