51,441 results match your criteria modified technique

The funny current in genetically modified mice.

Prog Biophys Mol Biol 2021 Jun 12. Epub 2021 Jun 12.

Institut de Génomique Fonctionnelle, Université de Montpellier, CNRS, INSERM, Montpellier, France; LabEx Ion Channels Science and Therapeutics (ICST), France. Electronic address:

Since its first description in 1979, the hyperpolarization-activated funny current (I) has been the object of intensive research aimed at understanding its role in cardiac pacemaker activity and its modulation by the sympathetic and parasympathetic branches of the autonomic nervous system. I was described in isolated tissue strips of the rabbit sinoatrial node using the double-electrode voltage-clamp technique. Since then, the rabbit has been the principal animal model for studying pacemaker activity and I for more than 20 years. Read More

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Top Ten Tips Palliative Care Clinicians Should Know About Trauma and Emergency Surgery.

J Palliat Med 2021 Jul;24(7):1072-1077

Division of Traumatology, Surgical Critical Care and Emergency Surgery, Department of Surgery, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, Pennsylvania, USA.

There is growing interest in, and need for, integrating palliative care (PC) into the care of patients undergoing emergency surgery and those with traumatic injury. Thus, PC consults for these populations will likely grow in the coming years. Understanding the nuances and unique characteristics of these two acutely ill populations will improve the care that PC clinicians can provide. Read More

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Tacrolimus-loaded chitosan nanoparticles for enhanced skin deposition and management of plaque psoriasis.

Carbohydr Polym 2021 Sep 23;268:118238. Epub 2021 May 23.

Department of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmacy, Ain Shams University, Cairo, Egypt. Electronic address:

Tacrolimus is a natural macrolide that exhibits an anti-proliferative action by T-lymphocytic cells inhibition. Hence, it was tested as a potential topical treatment to improve and control psoriatic plaques. In this study, for the first time the lipophilic tacrolimus in chitosan nanoparticles was used to achieve the desired response and dermal retention of the drug using a modified ionic gelation technique. Read More

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September 2021

Recording of electrocochleography from the facial nerve canal in mice.

J Neurosci Methods 2021 Jun 11;360:109256. Epub 2021 Jun 11.

Center for Hearing and Deafness, State University of New York at Buffalo, New York, United States of America.

Background: The ever-expanding arsenal of genetically modified mice has created experimental models for studying various mechanisms of deafness. Electrocochleography (ECochG) is a recording technique of cochlear potentials evoked by sound stimulation, which was widely used to evaluate the cochlear hearing function. However, there is currently a lack of information on long-term recording technology of ECochG in mice. Read More

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A modified diet to support conservation of the Atala hairstreak butterfly (Eumaeus atala Poey).

Zoo Biol 2021 Jun 14. Epub 2021 Jun 14.

Disney's Animals, Science, and Environment, The Walt Disney Company, Lake Buena Vista, Florida, USA.

Raising insects in a laboratory for release into the wild is a common conservation practice, but maintaining breeding colonies year-round can be limited by seasonal food availability. Food availability is particularly challenging for insects which depend on specific host plants. For example, our early efforts to rear the imperiled Atala hairstreak butterfly (Eumaeus atala Poey) resulted in colony failure during winter due to lack of food. Read More

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Modified double guiding catheter 'Ping Pong' technique to treat large coronary perforation: a case report.

Eur Heart J Case Rep 2021 May 16;5(5):ytab173. Epub 2021 May 16.

5 Hospital Drive, Level 2 Pharmacy, National Heart Centre Singapore 169609.

Background : Despite improvement in available tools and techniques, procedural complications like coronary perforation can occur during percutaneous coronary intervention (PCI). Severe proximal coronary perforations are usually caused by balloon and vessel size mismatch but can also occur with appropriately sized balloons or stents if the coronary vessel has very eccentric calcification or if there is negative remodelling of the vessel.

Case Summary : A 74-year-old man with a history of type II diabetes mellitus, hypertension, and chronic coronary syndrome (previous PCI 10 years before) presented with unstable angina of 2 weeks of duration. Read More

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Defining an Ultrasound-guided Regional Anesthesia Curriculum for Emergency Medicine.

AEM Educ Train 2021 Jul 11;5(3):e10557. Epub 2020 Dec 11.

Department of Emergency Medicine Michigan Medicine Ann Arbor MI USA.

Objectives: Ultrasound-guided regional anesthesia (UGRA) can be a powerful tool in the treatment of painful conditions commonly encountered in emergency medicine (EM) practice. UGRA can benefit patients while avoiding the risks of procedural sedation and opioid-based systemic analgesia. Despite these advantages, many EM trainees do not receive focused education in UGRA and there is no published curriculum specifically for EM physicians. Read More

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Detection of urinary microRNA biomarkers using diazo sulfonamide-modified screen printed carbon electrodes.

RSC Adv 2021 May 25;11(31):18832-18839. Epub 2021 May 25.

Wales Kidney Research Unit, Division of Infection & Immunity, School of Medicine, College of Biomedical and Life Sciences, Cardiff University Heath Park Cardiff CF14 4XN UK

This paper describes a straightforward electrochemical method for rapid and robust urinary microRNA (miRNA) quantification using disposable biosensors that can discriminate between urine from diabetic kidney disease (DKD) patients and control subjects. Aberrant miRNA expression has been observed in several major human disorders, and we have identified a urinary miRNA signature for DKD. MiRNAs therefore have considerable promise as disease biomarkers, and techniques to quantify these transcripts from clinical samples have significant clinical and commercial potential. Read More

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Endoscopic Management of Early Stage Middle Ear Paragangliomas - An Australian Case Series.

Otol Neurotol 2021 Jun 11. Epub 2021 Jun 11.

Department of Otolaryngology-Head & Neck Surgery, Fiona Stanley Hospital, Murdoch Joondalup Health Campus, Joondalup University of Western Australia Notre Dame University, WA, Australia Sydney Endoscopic Ear Surgery Research Group Discipline of Surgery, Sydney Medical School, University of Sydney Department of Otolaryngology-Head & Neck Surgery, Faculty of Medicine and Health Sciences, Macquarie University, Sydney Department of Otolaryngology-Head & Neck Surgery, Royal Prince Alfred Hospital, Sydney, NSW, Australia.

Objective: To analyze the outcomes of the endoscopic transcanal approach for removal of early stage middle ear paraganglioma tumors (MEPT).

Study Design: Cases series with chart review.

Setting: Two tertiary Australian Otology centers. Read More

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Integrin αβ-targeting ferritin nanocarrier traverses the blood-brain barrier for effective glioma chemotherapy.

J Nanobiotechnology 2021 Jun 13;19(1):180. Epub 2021 Jun 13.

Department of Biochemical Science and Technology, College of Life Science, National Taiwan University, AC2-414, No.1, Sec. 4, Roosevelt Rd., Taipei, 106319, Taiwan.

Background: Ferritin, the natural iron storage protein complex, self-assembles into a uniform cage-like structure. Human H-ferritin (HFn) has been shown to transverse the blood-brain barrier (BBB) by binding to transferrin receptor 1 (TfR1), which is abundant in endothelial cells and overexpressed in tumors, and enters cells via endocytosis. Ferritin is easily genetically modified with various functional molecules, justifying that it possesses great potential for development into a nanocarrier drug delivery system. Read More

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A modified simplex based direct search optimization algorithm for adaptive transversal FIR filters.

Sci Prog 2021 Apr-Jun;104(2):368504211025409

Department of Electrical and Computer Engineering, COMSATS University Islamabad, Islamabad, Pakistan.

In this paper, a novel modified optimization algorithm is presented, which combines Nelder-Mead (NM) method with a gradient-based approach. The well-known Nelder Mead optimization technique is widely used but it suffers from convergence issues in higher dimensional complex problems. Unlike the NM, in this proposed technique we have focused on two issues of the NM approach, one is shape of the simplex which is reshaped at each iteration according to the objective function, so we used a fixed shape of the simplex and we regenerate the simplex at each iteration and the second issue is related to reflection and expansion steps of the NM technique in each iteration, NM used fixed value of , that is, = 1 for reflection and = 2 for expansion and replace the worst point of the simplex with that new point in each iteration. Read More

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Modified Passerini-Glazel feminizing genitoplasty outcomes in adults: Two rare cases.

Int J Surg Case Rep 2021 Jun 10;84:106086. Epub 2021 Jun 10.

Urogynecology Division, Department of Obstetrics and Gynecology, Faculty of Medicine, University of Indonesia/Dr. Cipto Mangunkusumo Hospital, Jakarta, Indonesia. Electronic address:

Introduction: Modified Passerini-Glazel feminizing genitoplasty is typically performed in children with atypical genitalia. In our article, we have performed the procedure in adults with genital anomalies.

Case Presentation: The first case was a 22 years old woman who was planning to get married. Read More

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A CRISPR-based and post-amplification coupled SARS-CoV-2 detection with a portable evanescent wave biosensor.

Biosens Bioelectron 2021 Jun 7;190:113418. Epub 2021 Jun 7.

State Key Joint Laboratory of ESPC, Center for Sensor Technology of Environment and Health, School of Environment, Tsinghua University, Beijing, 100084, China. Electronic address:

The continuing pandemic of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection, which causes coronavirus disease 2019 (COVID-19), has spread globally and its reliable diagnosis is one of the foremost priorities for protecting public health. Herein a rapid (<1 h), easy-to-implement, and accurate CRISPR-based evanescent wave fluorescence biosensing platform for detection of SARS-CoV-2 is reported. The collateral effect of Cas13a is combined with a universal autonomous enzyme-free hybridization chain reaction (HCR) by designing a cleavage hairpin reporter, which is cleaved upon target recognition, and hence releasing the initiator sequence to trigger the downstream HCR circuits. Read More

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Application of a new serratus anterior plane block in modified radical mastectomy under ultrasound guidance: A prospective, randomized controlled trial.

J Clin Anesth 2021 Jun 9;74:110377. Epub 2021 Jun 9.

Department of Anesthesiology, The First Affiliated Hospital of Anhui Medical University, Hefei 230022, China. Electronic address:

Study Objectives: Post-operative pain is a significant concern following modified radical mastectomy in breast cancer patients. The serratus anterior plane block has recently been described as an effective technique for post-operative analgesia of modified radical mastectomy. The purpose of this study was to evaluate the analgesic efficacy and safety of a new serratus anterior plane (SAP) block for post-operative pain of mastectomy. Read More

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Flexible electrochemical uric acid and glucose biosensor.

Bioelectrochemistry 2021 Jun 6;141:107870. Epub 2021 Jun 6.

Frank Reidy Research Center for Bioelectrics, Department of Electrical and Computer Engineering, Old Dominion University, Norfolk, VA 23528, USA. Electronic address:

Fully integrated uric acid (UA) and glucose biosensors were fabricated on polydimethylsiloxane/polyimide platform by facile one step laser scribed technique. The laser scribed graphene (LSG) on the thin polyimide film was functionalized using pyrenebutanoic acid, succinimide ester (PBSE) to improve the electrochemical activity of the biosensors. The LSG was further decorated with platinum nanoparticles (PtNPs) to promote the electrocatalytic activity towards the oxidation of UA. Read More

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Transient electrophoresis of a conducting spherical particle embedded in an electrolyte saturated Brinkman medium.

Electrophoresis 2021 Jun 12. Epub 2021 Jun 12.

Department of Mathematics and Computer science, Faculty of Science, Alexandria University, Alexandria, Egypt.

The time-depended electrophoretic motion of a conducting spherical particle embedded in an arbitrary electrolyte solution saturated a porous medium is investigated. The porous medium is uniformly charged, and the embedded hard particle is charged with constant zeta potential or constant surface charge density. The unsteady modified Brinkman equation with an electric force term, which governs the fluid velocity field, is used to model the porous medium and solved by Laplace's transform technique. Read More

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The nexus between road transport intensity and road-related CO emissions in G20 countries: an advanced panel estimation.

Environ Sci Pollut Res Int 2021 Jun 11. Epub 2021 Jun 11.

School of Management and Economics, Beijing Institute of Technology, Beijing, 100081, China.

This study determines the dynamic linkages between road transport intensity, road transport passenger and road transport freight, and road carbon emissions in G20 countries in the presence of economic growth, urbanization, crude oil price, and trade openness for the period of 1990 to 2016, under the multivariate framework. This study employs the residual-based Kao and Westerlund cointegration technique to find long-run cointegration, and continuously updated bias-corrected (CUP-BC) and continuously updated fully modified (CUP-FM) methods to check the long-run elasticities between the variables. The long-run estimators' findings suggest a positive and significant impact of road transport intensity, road passenger transport, road freight transport on road transport CO emissions. Read More

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A modified transconjunctival technique for botulinum toxin chemodenervation of levator palpebrae superioris for corneal protection.

Eye (Lond) 2021 Jun 11. Epub 2021 Jun 11.

Cambridge University Hospitals, Cambridge, UK.

Background: Botulinum toxin (BTX) is useful for inducing temporary ptosis in patients with ocular surface disease. However, transcutaneous BTX often causes inadvertent superior rectus (SR) paresis. Furthermore, Muller's muscle is unaffected by transcutaneous BTX, resulting in lagophthalmos and incomplete ptosis. Read More

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Retrosigmoid transhorizontal fissure approach to lateral pontine cavernous malformation: comparison to transpetrosal presigmoid retrolabyrinthine approach.

J Neurosurg 2021 Jun 11:1-10. Epub 2021 Jun 11.

Objective: The retrosigmoid (RS) approach is a classic route used to access deep-seated brainstem cavernous malformation (CM). The angle of access is limited, so alternatives such as the transpetrosal presigmoid retrolabyrinthine (TPPR) approach have been used to overcome this limitation. Here, the authors evaluated a modification to the RS approach, horizontal fissure dissection by using the RS transhorizontal (RSTH) approach. Read More

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Rapid and highly selective electrochemical sensor based on ZnS/Au-decorated f-multi-walled carbon nanotube nanocomposites produced via pulsed laser technique for detection of toxic nitro compounds.

J Hazard Mater 2021 Jun 1;418:126269. Epub 2021 Jun 1.

Core-Facility Center for Photochemistry & Nanomaterials, Department of Chemistry (BK21 FOUR), Research Institute of Natural Sciences, Gyeongsang National University, Jinju 52828, Republic of Korea. Electronic address:

Novel ZnS/Au/f-multi-walled carbon nanotube (MWCNT) nanostructures were produced via a pulsed laser-assisted technique followed by a wet chemical process. ZnS nanospheres were synthesized via pulsed laser ablation of a Zn target in DMSO, which was used as a solvent and sulfur source. Notably, no additional sulfur sources, surfactants, or reducing agents were used during the synthesis. Read More

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Clinical and Radiographic Evaluation of Modified Transalveolar Two-Step Osteotome-Mediated Localized Maxillary Sinus Elevation: A Retrospective Computed Tomography Study with a 3-Year Follow-up.

Int J Oral Maxillofac Implants 2021 May-Jun;36(3):553-560

Purpose: This study aimed to report a practicable and noninvasive two-stage technique for sinus elevation and delayed implant insertion in the augmented site with residual bone height down to 3 mm or even lower.

Materials And Methods: The surgical technique employed a two-stage process for rehabilitation of posterior maxillary single-tooth edentulous areas, involving, in the first step, transcrestal maxillary sinus floor augmentation with a collagen sponge to fill the intrabony cavity resulting from the detachment of the sinus membrane; the second step consisted of another indirect sinus floor elevation using magnetoelectric surgery with immediate implant placement and no grafting material. Changes in bone height were evaluated by a comparison of the computed tomography scans acquired before treatment and after surgery (at 3 months and 5 years of the survey). Read More

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A double-closed wedge broken-line osteotomy for cubitus varus deformity.

Medicine (Baltimore) 2021 Jun;100(23):e26124

Department of Orthopedic Ward II, Shenzhen Children's Hospital, Shenzhen, China.

Abstract: Various osteotomy methods have been proposed in the treatment of cubitus varus. We designed an improved stepped osteotomy to achieve improved deformity correction. We refer to this new approach as double-closed wedge broken-line osteotomy and report a series of clinical and imaging results (deformity correction, range of motion [ROM], function, osteotomy healing, and complications) of patients with cubitus varus treated with this technique. Read More

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Cicatricial eyebrow restoration using the follicular unit extraction technique.

J Cosmet Dermatol 2021 Jun 11. Epub 2021 Jun 11.

Sultan Abdulhamid Han Education and Training Hospital Department of Plastic Reconstructive and Aesthetic Surgery.

Introduction: Cicatricial eyebrow loss that occurs after trauma or burns considerably reduces a person's self-confidence. Several methods, either surgical or nonsurgical, have been defined to reconstruct eyebrows. This study focused on eyebrow restoration using the atraumatic follicular unit extraction (FUE) technique in patients and emphasized certain details that should be considered during the treatment process. Read More

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Is the modified Ponseti method effective in treating atypical and complex clubfoot? A systematic review.

Int Orthop 2021 Jun 10. Epub 2021 Jun 10.

Department of Orthopedic Surgery, King Faisal Specialist Hospital and Research Centre, Riyadh, Saudi Arabia.

Purpose: In 2006, Ponseti modified the standard technique to treat cases of "atypical" and "complex" clubfoot. To determine the outcomes of Ponseti's modified method to treat complex idiopathic clubfoot patients, we asked the following: (1) What is the deformity correction success rate? (2) What is the relapse rate after the correction? (3) What is the incidence of complications?

Materials And Methods: We performed a systematic review by searching the EMBASE, MEDLINE, Cochrane Library, and Web of Science databases from inception to March 1, 2021. All studies on idiopathic, complex, and atypical clubfoot that assessed Ponseti's modified technique were included. Read More

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Anatomical repair of distal biceps brachial tendon lesions using double-incision surgical approach.

Rev Esp Cir Ortop Traumatol 2021 Jul-Aug;65(4):305-311

Unidad de Investigación, Clínica CEMTRO, Madrid, Spain. Electronic address:

Background: and objective The aim of this work was to evaluate clinical outcomes of patients with biceps brachii tendon rupture who underwent a surgical tendon reconstruction using our own modified double approach technique.

Patients: The study was performed in 20 patients (21 cases) with biceps brachii tendon rupture. Patients were treated with our own double-approach surgical technique consisting of 2 minimum incisions. Read More

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September 2019

Novel nano-engineered environmental sensor based on polymelamine/graphitic-carbon nitride nanohybrid material for sensitive and simultaneous monitoring of toxic heavy metals.

J Hazard Mater 2021 May 31;418:126267. Epub 2021 May 31.

Department of Medicinal and Applied Chemistry, Kaohsiung Medical University (KMU), Kaohsiung City 807, Taiwan; Research Center for Environmental Medicine, Kaohsiung Medical University (KMU), Kaohsiung City 807, Taiwan; Department of Medical Research, Kaohsiung Medical University Hospital (KMUH), Kaohsiung City 807, Taiwan; Department of Chemistry, National Sun Yat-sen University (NSYSU), Kaohsiung City 804, Taiwan; PhD Program of Aquatic Science and Technology, College of Hydrosphere Science, National Kaohsiung University of Science and Technology (NKUST), Kaohsiung City 807, Taiwan. Electronic address:

Heavy metal ions (HMIs) pollution is always a serious issue worldwide. Therefore, monitoring HMIs in environmental water is an important and challenging step to ensure environmental health and human safety. In this study, we spotlight an effortless, single-step in-situ electrochemical polymerization deposition technique to fabricate a novel, low-cost, efficient, nano-engineered poly(melamine)/graphitic-carbon nitride nanonetwork (PM/g-CN) modified screen-printed carbon electrode (SPE) for sensitive, selective, and simultaneous electrochemical monitoring of toxic HMIs in environmental waters. Read More

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Exer-gaming reduces fall risk and improves mobility after stroke.

J Pak Med Assoc 2021 Jun;71(6):1673-1675

Ripah International University, Lahore Campus, Pakistan.

The current study evaluated the effect of virtual reality based balance training in 30 stroke patients recruited via purposive sampling technique for a clinical trial. Sealed envelope method was used to randomly allocate patients into two groups, i.e. Read More

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Dosimetric evaluation of a patient-specific 3D-printed oral positioning stent for head-and-neck radiotherapy.

Phys Eng Sci Med 2021 Jun 10. Epub 2021 Jun 10.

Royal Brisbane and Women's Hospital, Herston, QLD, 4029, Australia.

As head-and-neck radiotherapy treatments become more complex and sophisticated, and the need to control and stabilise the positioning of intra-oral anatomy becomes more important, leading the increasing use of oral positioning stents during head-and-neck radiotherapy simulation and delivery. As an alternative to the established practice of creating oral positioning stents using wax, this study investigated the use of a 3D printing technique. An Ender 5 3D printer (Creality 3D, Shenzhen, China) was used, with PLA+ "food-safe" polylactic acid filament (3D Fillies, Dandenong South, Australia), to produce a low-density 3D printed duplicate of a conventional wax stent. Read More

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Volar Plating versus Headless Compression Screw Fixation of Scaphoid Nonunions: A Meta-analysis of Outcomes.

J Wrist Surg 2021 Jun 3;10(3):255-261. Epub 2021 Jan 3.

Department of Orthopaedic Surgery, The Rothman Institute at Thomas Jefferson University, Philadelphia, Pennsylvania.

 Headless compression screw fixation with bone grafting has been the mainstay of treatment for scaphoid nonunion for the past several decades. Recently, locked volar plate fixation has gained popularity as a technique for scaphoid fixation, especially for recalcitrant or secondary nonunions.  The purpose of this meta-analysis was to compare union rates and clinical outcomes between locked volar plate fixation and headless compression screw fixation for the treatment of scaphoid nonunions. Read More

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Modified technique for double J stent removal.

Radiol Bras 2021 May-Jun;54(3):204-205

Interventional Radiology Unit, "F. Miulli" Regional General Hospital, Acquaviva dele Fonti (BA), Italy.

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