151 results match your criteria modified pullulan


Effects of pullulan on the biomechanical and anti-collapse properties of dicalcium phosphate dihydrate bone cement.

J Biomater Appl 2021 Jun 1:8853282211020158. Epub 2021 Jun 1.

School of Chemical Engineering, Sichuan University, Chengdu, China.

In this work, a modified dicalcium phosphate dihydrate (DCPD) bone cement with unique biodegradable ability in a calcium phosphate cement system was prepared by the hydration reaction of monocalcium phosphate monohydrate and calcium oxide and integration with pullulan (Pul), a non-toxic, biocompatible, viscous, and water-soluble polysaccharide that has been successfully used to improve defects in DCPD bone cement, especially its rapid solidification, fragile mechanical properties, and easy collapse. The effect of different contents of Pul on the structure and properties of DCPD were also studied in detail. The modified cement was characterised by X-ray diffraction, Fourier-transform infrared spectroscopy, scanning electron microscopy, ultraviolet-visible absorption, X-ray photoelectron spectroscopy analysis, and rheological property measurements. Read More

View Article and Full-Text PDF

Stability and bioaccessibility of curcumin emulsions stabilized by casein hydrolysates after maleic anhydride acylation and pullulan glycation.

J Dairy Sci 2021 Aug 10;104(8):8425-8438. Epub 2021 May 10.

Engineering Research Center of Bio-Process, Ministry of Education, Hefei University of Technology, Hefei, Anhui 230009, P. R. China; School of Food and Biological Engineering, Hefei University of Technology, Hefei, Anhui 230009, P. R. China; State Key Laboratory of Hybrid Rice, Hunan Hybrid Rice Research Center, Changsha 410125, P. R. China. Electronic address:

The effects of maleic anhydride (MA) acylation and pullulan glycation on casein hydrolysates (CH) and the physicochemical stability of modified or unmodified CH-stabilized emulsions were explored. Compared with casein, the solubility of CH was improved, and CH (hydrolysis degree 4%) exhibited the optimal emulsifying properties. After the acylation of MA, degrees of acylation (DA) increased with increasing addition of MA. Read More

View Article and Full-Text PDF

Characterization of the Micelle Formed by a Hydrophobically Modified Pullulan in Aqueous Solution: Size Exclusion Chromatography.

Polymers (Basel) 2021 Apr 11;13(8). Epub 2021 Apr 11.

Department of Macromolecular Science, Osaka University, Toyonaka Osaka 560-0043, Japan.

Size exclusion chromatography equipped with a multi-angle, light-scattering online detector (SEC-MALS) measurements were carried out on a hydrophobically modified pullulan (PUL-OSA) with degrees of substitution () of 0.14, 0.2, and 0. Read More

View Article and Full-Text PDF

Highly efficient removal of antibiotic from biomedical wastewater using Fenton-like catalyst magnetic pullulan hydrogels.

Carbohydr Polym 2021 Jun 15;262:117951. Epub 2021 Mar 15.

State Key Laboratory of Ophthalmology, Optometry and Vision Science, School of Ophthalmology and Optometry, School of Biomedical Engineering, Wenzhou Medical University, Wenzhou, 325027, China. Electronic address:

Magnetic nanoparticles that can be employed as Fenton-like catalysts Fenton-like catalysts are attractive materials for degrading antibiotics. In this study, we facilely prepared novel magnetic pullulan (MP) hydrogels by doping modified magnetic nanoparticles into pullulan matrices, which could enhance catalytic degradation performance and strengthen the stability of resulting hydrogels. This is the first time that MP hydrogels have been fabricated successfully and used as Fenton-like catalysts for tetracycline hydrochloride (TCH) degradation. Read More

View Article and Full-Text PDF

Methacrylated pullulan/polyethylene (glycol) diacrylate composite hydrogel for cartilage tissue engineering.

J Biomater Sci Polym Ed 2021 Jun 23;32(8):1057-1071. Epub 2021 Mar 23.

Center for Joint Surgery, Southwest Hospital, Third Military Medical University, Chongqing, China.

Pullulan hydrogels are widely used in tissue engineering and drug delivery. However, these hydrogels do not meet the requirements of articular cartilage repair because of their fast degradation rate and poor mechanical strength. Herein, we fabricated a hybrid hydrogel system by combining pullulan with synthetic polymers polyethylene (glycol) diacrylate (PEGDA). Read More

View Article and Full-Text PDF

Electrospinning of Octenylsuccinylated Starch-Pullulan Nanofibers from Aqueous Dispersions.

Carbohydr Polym 2021 Apr 21;258:116933. Epub 2020 Aug 21.

Department of Food Science, Pennsylvania State University, 341 Food Science Building, University Park, PA, 16802, USA. Electronic address:

We aimed to develop a greener process for dry-electrospinning food-grade modified starch through the elimination of organic solvents. The rheological properties and electrospinnability of aqueous dispersions of commercial octenylsuccinylated (OS) starches with various molecular weight (Mw) were investigated, yet only nanofibers with beads or defects could be obtained from OS starch with the highest Mw, i.e. Read More

View Article and Full-Text PDF

[Effect of ginsenoside Rg_3 nanostructured lipid carrier modified by pullulan on promoting absorption and its anti-tumor evaluation in vitro].

Zhongguo Zhong Yao Za Zhi 2020 Nov;45(21):5184-5192

Department of Pharmacy, Suqian First Hospital, Suqian Branch Jiangsu Province Hospital Suqian 223800, China.

Ginsenoside Rg_3 is widely used in clinical practice as an anti-tumor adjuvant drug, but its application is limited due to its poor oral absorption. In this study, we intended to construct a ginsenoside Rg_3 nanostructured lipid carrier modified by the pullulan(PUL-Rg_3-NLC) to improve the adhesion properties of ginsenoside Rg_3, promote the drug uptake and improve the anti-tumor efficacy. PUL-Rg_3-NLC was characterized by morphology, particle size and Zeta potential. Read More

View Article and Full-Text PDF
November 2020

Fabrication and characterization of Pickering emulsion gels stabilized by zein/pullulan complex colloidal particles.

J Sci Food Agric 2021 Jul 22;101(9):3630-3643. Epub 2020 Dec 22.

Longping Branch Graduate School, Hunan University, Changsha, China.

Background: Zein particles are unsuitable as stabilizers of Pickering emulsions because of their high hydrophobicity. However, few studies have reported on the use of a strong hydrophilic neutral polysaccharide to regulate its wettability. In this work, zein/pullulan complex particles (ZPPs) were formulated by an anti-solvent method to fabricate Pickering emulsions. Read More

View Article and Full-Text PDF

Chemical Modification of Pullulan Exopolysaccharide by Grafting Poly(3-hydroxybutyrate--3-hydroxyvalerate) (PHBHV) via Click Chemistry.

Polymers (Basel) 2020 Oct 29;12(11). Epub 2020 Oct 29.

Department of Chemical Engineering, Engineering School of Lorena, University of São Paulo, EEL-USP, Lorena 12602-810, SP, Brazil.

Biodegradable and biocompatible copolymers have been often studied for the development of biomaterials for drug delivery systems. In this context, this work reports the synthesis and characterization of a novel pullulan--poly(3-hydroxybutyrate--3-hydroxyvalerate) (Pull-PHBHV) graft copolymer using click chemistry. Well-defined and functional pullulan backbones containing azide groups (PullN) previously prepared by our group were successfully used for this purpose and propargyl-terminated poly(3-hydroxybutyrate--3-hydroxyvalerate) was prepared via transesterification using propargyl alcohol as a chain transfer agent. Read More

View Article and Full-Text PDF
October 2020

Emulsifying properties and structure characteristics of octenyl succinic anhydride-modified pullulans with different degree of substitution.

Carbohydr Polym 2020 Dec 18;250:116844. Epub 2020 Aug 18.

State Key Laboratory of Food Science and Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi, 214122, Jiangsu, China.

Pullulan was successfully esterified with octenyl succinic anhydride (OSA) in aqueous alkaline solutions. The degree of substitution (DS) was regulated from 0.0163, 0. Read More

View Article and Full-Text PDF
December 2020

Pullulan derivative with cationic and hydrophobic moieties as an appropriate macromolecule in the synthesis of nanoparticles for drug delivery.

Int J Biol Macromol 2020 Dec 15;164:4487-4498. Epub 2020 Sep 15.

"Petru Poni" Institute of Macromolecular Chemistry, Gr. Ghica Voda 41A, 700487 Iasi, Romania.

A new amphiphilic pullulan derivative (DBAP-PO) was obtained by grafting tertiary butyl amine and octanoyl groups on the pullulan backbone as cationic and hydrophobic moieties, respectively. The structural characteristics of the modified polymer were investigated by FT-IR and H and C NMR spectroscopy. The self-association ability in aqueous solution of DBAP-PO was studied by viscosity and fluorescence methods. Read More

View Article and Full-Text PDF
December 2020

Chemical modification of pullulan exopolysaccharide by octenyl succinic anhydride: Optimization, physicochemical, structural and functional properties.

Int J Biol Macromol 2020 Dec 22;164:3485-3495. Epub 2020 Aug 22.

Bioprocessing and Biodetection Laboratory, Department of Food Science and Engineering, University of Tehran, Karaj 31587-77871, Iran.

Pullulan (PU) is an exo-polysaccharide used in the food and pharmaceutical industries. However, the use of PU in different industries is limited due to its highly hydrophilic nature and consequently weakness in surface properties which can be remedied by its chemical modification with octenyl succinic anhydride (OSA). For this purpose, PU modification with OSA was optimized and the results showed that the maximum degree of substitution (0. Read More

View Article and Full-Text PDF
December 2020

Ultrasound Combined with Core Cross-Linked Nanosystem for Enhancing Penetration of Doxorubicin Prodrug/Beta-Lapachone into Tumors.

Int J Nanomedicine 2020 7;15:4825-4845. Epub 2020 Jul 7.

State Key Laboratory of Ultrasound in Medicine and Engineering, College of Biomedical Engineering, Chongqing Medical University, Chongqing 400016, People's Republic of China.

Background: Nanosized drug delivery systems (NDDSs) have shown excellent prospects in tumor therapy. However, insufficient penetration of NDDSs has significantly impeded their development due to physiological instability and low passive penetration efficiency.

Methods: Herein, we prepared a core cross-linked pullulan-modified nanosized system, fabricated by visible-light-induced diselenide bond cross-linked method for transporting β-Lapachone and doxorubicin prodrug (boronate-DOX, BDOX), to improve the physiological stability of the NDDSs for efficient passive accumulation in tumor blood vessels (β-Lapachone/BDOX-CCS). Read More

View Article and Full-Text PDF

Cationised dextran and pullulan modified with diethyl aminoethyl methacrylate for gene delivery in cancer cells.

Carbohydr Polym 2020 Aug 16;242:116426. Epub 2020 May 16.

Biosurface Technology Division, Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Thiruvananthapuram, Kerala, India.

This work describes the synthesis and characterisation of cationised dextran and pullulan modified with diethyl aminoethyl methacrylate (DEAEM) for gene delivery in cancer cells. To dextran and pullulan, PEI was conjugated to impart cationicity. These cationised polysaccharides were then modified with DEAEM monomer via Michael addition reaction and synthesised four different derivatives viz DPD I, DPD II, PPD I and PPD II. Read More

View Article and Full-Text PDF

Bioactive and biostable hyaluronic acid-pullulan dermal hydrogels incorporated with biomimetic hydroxyapatite spheres.

Mater Sci Eng C Mater Biol Appl 2020 Jul 8;112:110906. Epub 2020 Apr 8.

Department of Animal, Avian and Marine Biotechnology, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran.

In this study, hyaluronic acid-pullulan injectable hydrogels were incorporated with biomimetic hydroxyapatite spheres and were modified using silane coupling agents in order to improve the physicochemical, mechanical, and biological performance of hydrogels. So the biomimetic hydroxyapatite spheres were synthesized through immersion of gelatin-siloxane microspheres in the simulated body fluid. The results of field emission scanning electron microscopy, energy dispersive X-ray spectroscopy, and X-ray diffraction spectra confirmed the formation of hydroxyapatite on the surface of hybrid spheres. Read More

View Article and Full-Text PDF

Preparation and evaluation of curcumin grafted hyaluronic acid modified pullulan polymers as a functional wound dressing material.

Carbohydr Polym 2020 Jun 19;238:116195. Epub 2020 Mar 19.

College of Pharmacy & Key Laboratory of Pharmaceutical Quality Control of Hebei Province, Hebei University, Baoding, 071002, PR China. Electronic address:

Curcumin grafted hyaluronic acid modified pullulan polymers (Cur-HA-SPu) by chemical conjugation was designed and prepared, and its film may be used to accelerate wound healing and help to fight infection. The synthesis of Cur-HA-SPu polymer was characterized by FT-IR, H NMR and DSC. Cur-HA-SPu film has a higher swelling ratio than that of HA-SPu film. Read More

View Article and Full-Text PDF

Crosslinked nanogel-based porous hydrogel as a functional scaffold for tongue muscle regeneration.

J Biomater Sci Polym Ed 2020 07 16;31(10):1254-1271. Epub 2020 Jun 16.

Department of Polymer Chemistry, Graduate School of Engineering, Kyoto University, Katsura, Kyoto, Japan.

Surgical resection in tongue cancer can impair speech and swallowing, reducing quality of life. There is a need for biomaterials that can regenerate tongue muscle tissue defects. Ideally, such a biomaterial would allow controlled release of therapeutic proteins, support the survival and differentiation of therapeutic cells, and promote tongue muscle regeneration . Read More

View Article and Full-Text PDF

Engineering butylglyceryl-modified polysaccharides towards nanomedicines for brain drug delivery.

Carbohydr Polym 2020 May 21;236:116060. Epub 2020 Feb 21.

School of Pharmacy and Biomedical Sciences, University of Portsmouth, St Michael's Building, White Swan Road, PO1 2DT, United Kingdom. Electronic address:

Colloidal systems prepared from carbohydrates are subject of intense research due to their potential to enhance drug permeability through biological membranes, however their characteristics and performance are never compared directly. Here we report the results of a comparative investigation of a series of butylglyceryl-modified polysaccharides (chitosan, guar gum, and pullulan) that were formulated into nanoparticles and loaded with a range of model actives (Doxorubicin, Rhodamine B, Angiotensin II). Butylglyceryl-modified guar gum and corresponding pullulan nanocarriers were more stable at physiological pH compared to those obtained from modified chitosan, and studies of the in-vitro interactions with mouse brain endothelial cells (bEnd3) indicated an increased biological membrane permeability and lack of toxicity at application-relevant concentrations. Read More

View Article and Full-Text PDF

Synthesis of amphiphilic pullulan-graft-poly(ε-caprolactone) via click chemistry.

Int J Biol Macromol 2020 Feb 24;145:701-711. Epub 2019 Dec 24.

Department of Chemical Engineering, Engineering School of Lorena, University of São Paulo, EEL-USP, Lorena, SP, Brazil. Electronic address:

Chemical modification of natural polymers has been commonly employed for the development of new bio-based materials, aiming at adjusting specific properties such as solubility, biodegradability, thermal stability and mechanical behavior. Among all natural polymers, polysaccharides are promising materials, in which biodegradability, processability and bioreactivity make them suitable for biomedical applications. In this context, this work describes the synthesis and characterization of a novel amphiphilic pullulan-g-poly(ε-caprolactone) (Pull-g-PCL) graft copolymer. Read More

View Article and Full-Text PDF
February 2020

Antigen Delivery to Antigen-Presenting Cells for Adaptive Immune Response by Self-Assembled Anionic Polysaccharide Nanogel Vaccines.

Biomacromolecules 2020 02 12;21(2):621-629. Epub 2019 Dec 12.

Department of Polymer Chemistry, Graduate School of Engineering , Kyoto University , Kyoto 615-8510 , Japan.

Although current vaccine technology induces sufficient antibody responses to prophylactically ward off viral infections, an anticancer vaccine that directs the patient's immune system to directly fight extant malignant cells will require inducing Th1 and cytotoxic T lymphocyte responses in addition to antibody-mediated activities. Thus, new mechanisms are necessary to deliver antigen to cells in the lymphatic system that will induce these responses. To this end, we have developed a cholesterol-bearing pullulan (CHP) self-assembly nanogel of less than 100 nm, which we have now further modified to be anionic by carboxyl group substitution. Read More

View Article and Full-Text PDF
February 2020

Green construction of recyclable amino-tannic acid modified magnetic nanoparticles: Application for β-glucosidase immobilization.

Int J Biol Macromol 2020 Jul 13;154:1366-1374. Epub 2019 Nov 13.

Bioprocessing and Biodetection Laboratory, Department of Food Science and Engineering, University of Tehran, Karaj 31587-77871, Iran.

The β-glucosidase (BGL) enzyme in food industry is great interest due to its role in food conversion to produce functional food products. In this study, the BGL was covalently immobilized onto amino-tannic acid modified FeO magnetic nanoparticles (ATA-FeO MNPs) as biocompatible nanoplatform by modified poly-aldehyde pullulan (PAP) as a cross-linker to enhance the ability and strength of the nanoparticle connection to the enzyme. The properties of support were subsequently characterized by dynamic light scattering (DLS), transmission electron microscopy (TEM), Fourier transformed infrared (FTIR), X-ray diffraction (XRD), and vibrating sample magnetometer (VSM). Read More

View Article and Full-Text PDF

pH-sensitive and pluronic-modified pullulan nanogels for greatly improved antitumor in vivo.

Int J Biol Macromol 2019 Oct 1;139:277-289. Epub 2019 Aug 1.

Engineering Research Center for Biomedical Materials, Anhui Key Laboratory of Modern Biomanufacturing, School of Life Sciences, Anhui University, 111 Jiulong Road, Hefei, Anhui Province 230601, PR China. Electronic address:

It remains a crucial challenge to achieve efficient cellular uptake in tumor cells for nanoscale drug delivery systems. This work described that two multi-functional pullulan nanogels were prepared by co-polymerization between methacrylated pullulan (Pullulan-M) and different crosslink agents, an acid-labile ortho ester-modified pluronic (L61-MOE) or non-acid-sensitive methacrylated pluronic (L61-M). The prepared nanogels showed a regular spherical structure with the size about 200 nm measured by dynamic light scattering and transmission electron microscopy (TEM). Read More

View Article and Full-Text PDF
October 2019

Effect of lipopolysaccharide addition on the gene transfection of spermine-introduced pullulan-plasmid DNA complexes for human mesenchymal stem cells.

J Biomater Sci Polym Ed 2019 11 8;30(16):1542-1558. Epub 2019 Aug 8.

Laboratory of Biomaterials, Department of Regeneration Science and Engineering, Institute for Frontier Life and Medical Sciences, Kyoto University , Japan.

The objective of this study is to investigate the effect of lipopolysaccharide (LPS) addition on the gene transfection of human mesenchymal stem cells (hMSC). hMSC were treated with the LPS at different concentrations and the complex of spermine-introduced pullulan and luciferase plasmid DNA for 3 h. The maximum level of gene expression was observed for hMSC treated with a certain concentration range of LPS. Read More

View Article and Full-Text PDF
November 2019

An enzymatically controlled mucoadhesive system for enhancing flavour during food oral processing.

NPJ Sci Food 2019 1;3:11. Epub 2019 Jul 1.

2Division of Food, Nutrition and Dietetics, School of Biosciences, University of Nottingham, Sutton Bonington Campus, Leicestershire, UK.

While a good mucoadhesive biopolymer must adhere to a mucus membrane, it must also have a good unloading ability. Here, we demonstrate that the biopolymer pullulan is partially digested by human salivary α-amylase, thus acting as a controlled release system, in which the enzyme triggers an increased release of flavour. Our oral processing simulations have confirmed an increase in the bioavailability of aroma and salt compounds as a function of oral pullulan degradation, although the release kinetics suggest a rather slow process. Read More

View Article and Full-Text PDF

Comparison between conventional and frit-inlet channels in separation of biopolymers by asymmetric flow field-flow fractionation.

Analyst 2019 Aug 21;144(15):4559-4568. Epub 2019 Jun 21.

Department of Food Technology, Engineering and Nutrition, Faculty of Engineering, Lund University, PO Box 124, S-22100 Lund, Sweden.

Asymmetric flow field-flow fractionation (AF4) is a separation technique in which a focusing/relaxation step is used after the sample is injected onto the separation channel. During the focusing/relaxation step, the sample is focused by two counter-directed flows. This allows sample components to establish a diffusion-dependent equilibrium concentration profile. Read More

View Article and Full-Text PDF

Electrospinning and electrospraying technologies for food applications.

Adv Food Nutr Res 2019 25;88:167-234. Epub 2019 Mar 25.

Nano-BioScience Research Group, DTU-Food, Technical University of Denmark, Lyngby, Denmark.

Electrospinning and electrospraying are versatile techniques for the production of nano- to micro-scale fibers and particles. Over the past 2 decades, significant progresses have been made to advance the fundamental understandings of these electrohydrodynamic processes. Researchers have investigated different polymeric and non-polymeric substrates for producing submicron electrospun/electrosprayed materials of unique morphologies and physicochemical properties. Read More

View Article and Full-Text PDF
December 2019

Electrospun nanofiber mats from aqueous starch-pullulan dispersions: Optimizing dispersion properties for electrospinning.

Int J Biol Macromol 2019 Jul 1;133:1168-1174. Epub 2019 May 1.

Department of Food Science, Pennsylvania State University, University Park, PA 16802, United States. Electronic address:

A green method to fabricate starch-based nanofibers is provided. High-temperature (≈162 °C) was used to destructure high-amylose starch in water. Sodium palmitate was added to enhance the stability of high-amylose starch in water at room temperature and increase the conductivity of the electrospinning dope. Read More

View Article and Full-Text PDF

Investigating the potential of carboxymethyl pullulan for protecting the rabbit eye from systematically induced precorneal tear film damage.

Exp Eye Res 2019 07 17;184:91-100. Epub 2019 Apr 17.

Pharmaceutics Division, Department of Pharmaceutical Sciences and Drug Research, Punjabi University, Patiala, 147 002, Punjab, India. Electronic address:

The present investigation was aimed to develop a rabbit model for protecting the rabbit eye from systematically induced precorneal tear film (PTF) damage, evaluation of carboxymethyl pullulan for its protective action against PTF damage and its curative potential. For the same, pullulan was modified by carboxymethylation and structural modification was confirmed by spectral attributes. Further, the carboxymethyl pullulan (CMP) solutions (0. Read More

View Article and Full-Text PDF

Evaluation of human dental pulp stem cells behavior on a novel nanobiocomposite scaffold prepared for regenerative endodontics.

Mater Sci Eng C Mater Biol Appl 2019 Jul 8;100:928-948. Epub 2019 Mar 8.

Department of Biotechnology, Middle East Technical University, Ankara 06800, Turkey; Department of Engineering Sciences, Middle East Technical University, Ankara 06800, Turkey; BIOMATEN, CoE in Biomaterials and Tissue Engineering, METU, Ankara 06800, Turkey. Electronic address:

Dental caries is a dental disease affecting public health, which results in many socio-economic consequences. This disease causes loss of tooth hard tissue and subsequent inflammation and loss of the dental pulp. In this study, it was aimed to develop and characterize boron (B) modified bioactive glass nanoparticles (BG-NPs) containing cellulose acetate/oxidized pullulan/gelatin (CA/ox-PULL/GEL) three dimensional scaffolds with tubular morphology for dentin regeneration. Read More

View Article and Full-Text PDF

The antiatherogenic effect of new biocompatible cationically modified polysaccharides: chitosan and pullulan - the comparison study.

J Physiol Pharmacol 2018 Dec 18;69(6). Epub 2019 Mar 18.

Chair of Pharmacology, Jagiellonian University, Medical College, Cracow, Poland.

Pullulan and chitosan are biocompatible polysaccharides obtained from natural sources with many biomedical applications. Cationically modified polymers, such as chitosan and pullulan after covalent attachment of glycidyltrimethylammonium chloride (GTMAC), showed beneficial biological properties. In the present study, it was clearly demonstrated and confirmed that both cationically modified polysaccharides (chitosan-GTMAC and pullulan-GTMAC) have the antiatherosclerotic potential by inhibition of atherosclerotic plaque development and controlling the expression of genes involved in lipid metabolism. Read More

View Article and Full-Text PDF
December 2018