523 results match your criteria modified hodge

Rigid monoclonal antibodies improve detection of SARS-CoV-2 nucleocapsid protein.

MAbs 2021 Jan-Dec;13(1):1905978

Molecular Biophysics and Integrated Bioimaging, Lawrence Berkeley National Laboratory, Berkeley, CA, USA.

Monoclonal antibodies (mAbs) are the basis of treatments and diagnostics for pathogens and other biological phenomena. We conducted a structural characterization of mAbs against the N-terminal domain of nucleocapsid protein (NP) from SARS-CoV-2 using small-angle X-ray scattering and transmission electron microscopy. Our solution-based results distinguished the mAbs' flexibility and how this flexibility affects the assembly of multiple mAbs on an antigen. Read More

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Detection of carbapenemase-producing bacteria in a public healthcare center from Venezuela.

J Infect Dev Ctries 2020 Dec 27;15(1):163-167. Epub 2020 Dec 27.

School of Medicine, Medical Faculty, University of Zulia, Maracaibo, Venezuela.

Introduction: The dramatic increase in the prevalence and clinical impact of infections caused by Carbapenemase-Producing Bacteria in the nosocomial setting in Latin America represents an emerging challenge to public health. The present study detected carbapenemase-producing Gram-negative bacteria in patients from a Hospital from Venezuela, by phenotypic and genotypic methods.

Methodology: The bacterial identification was carried out using conventional methods. Read More

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December 2020

The Cancer Prevention Study-3 FFQ Is a Reliable and Valid Measure of Nutrient Intakes among Racial/Ethnic Subgroups, Compared with 24-Hour Recalls and Biomarkers.

J Nutr 2021 Mar;151(3):636-648

Department of Population Science, American Cancer Society, Atlanta, GA, USA.

Background: Valid assessment of dietary intake in diverse populations is important for studies of chronic disease risk in the United States.

Objectives: We evaluated the reproducibility and validity of a food frequency questionnaire (FFQ) modified for the American Cancer Society's Cancer Prevention Study-3 (CPS-3) prospective cohort, among a racially/ethnically diverse subgroup.

Methods: The Diet Assessment Substudy included 677 CPS-3 participants (64% women; 61% non-Hispanic white, 24% non-Hispanic black, 15% Hispanic), aged 31-70 y, who completed 2 FFQs 1 y apart (FFQ1, FFQ2), 4-6 telephone-administered 24-h dietary recalls (24HRs), and 2 fasting blood samples and 24-h urine collections ∼6 mo apart in the interim. Read More

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Rigid monoclonal antibodies improve detection of SARS-CoV-2 nucleocapsid protein.

bioRxiv 2021 Jan 13. Epub 2021 Jan 13.

Monoclonal antibodies (mAbs) are the basis of treatments and diagnostics for pathogens and other biological phenomena. We conducted a structural characterization of mAbs against the N-terminal domain of nucleocapsid protein (NP ) from SARS-CoV-2 using small angle X-ray scattering (SAXS). Our solution-based results distinguished the mAbs' flexibility and how this flexibility impacts the assembly of multiple mAbs on an antigen. Read More

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January 2021

Comparison of four low-cost carbapenemase detection tests and a proposal of an algorithm for early detection of carbapenemase-producing Enterobacteriaceae in resource-limited settings.

PLoS One 2021 12;16(1):e0245290. Epub 2021 Jan 12.

Department of Biochemistry and Clinical Chemistry, Faculty of Medicine, University of Kelaniya, Ragama, Sri Lanka.

Rapidly progressing antibiotic resistance is a great challenge in therapy. In particular, the infections caused by carbapenem-resistant Enterobacteriaceae (CRE) are exceedingly difficult to treat. Carbapenemase production is the predominant mechanism of resistance in CRE. Read More

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January 2021

Comparison of Genotypic and Phenotypic Methods of Metallo-β- lactamase Detection in spp.

J Glob Infect Dis 2020 Jul-Sep;12(3):141-143. Epub 2020 Aug 29.

Department of Orthopaedics, JPNA Trauma Centre, All India Institute of Medical Sciences, New Delhi, India.

Introduction: MBL containing genes have been reported in all GNBs including spp since 1990s which are worrisome as they are transmitted by mobile genetic elements. Thus, early detection of MBL encoding organisms is necessary. The current study was designed to identify the most sensitive cost-effective test which could be used as a screening test for detection of cabapenamase producing isolates. Read More

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Application Value of Triton X-100 to Modified Hodge Test and Carbapenem Inactivation Method in the Detection of Carbapenemase.

Infect Drug Resist 2020 24;13:4283-4288. Epub 2020 Nov 24.

Department of Clinical Laboratory, The First Affiliated Hospital of Anhui Medical University, Hefei City 230022, People's Republic of China.

Aim: To compare the sensitivity and specificity before and after the addition of Triton X-100 in the modified Hodge test (MHT) and carbapenem inactivation method (CIM) for the detection of carbapenemase in

Materials And Methods: A total of 135 isolates of (83 carbapenem-resistant and 52 carbapenem-sensitive) were selected and the carbapenemase genotypes were detected using PCR. Carbapenemase phenotypes were tested using the MHT, Triton-MHT (THT), CIM, modified CIM (mCIM), and Triton-CIM (TCIM). Different concentrations (0. Read More

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November 2020

Phenotypic and Genotypic Features of Harboring Carbapenemases in Egypt: OXA-48-Like Carbapenemases as an Investigated Model.

Antibiotics (Basel) 2020 Nov 28;9(12). Epub 2020 Nov 28.

Department of Microbiology and Immunology, Faculty of Pharmacy (Girls), Al-Azhar University, Cairo 11884, Egypt.

This study aimed at the characterization of carbapenem-resistant isolates focusing on typing of the genes. Additionally, the correlation between the resistance pattern and biofilm formation capacity of the carbapenem-resistant isolates was studied. The collected isolates were assessed for their antimicrobial resistance and carbapenemases production by a modified Hodge test and inhibitor-based tests. Read More

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November 2020

Carbapenemase OXA-423: A Novel OXA-23 Variant in .

Infect Drug Resist 2020 10;13:4069-4075. Epub 2020 Nov 10.

Department of Clinical Laboratory, Yijishan Hospital of Wannan Medical College, Wuhu, People's Republic of China.

Purpose: A novel variant of OXA-23, named OXA-423, was identified in an clinical isolate. The aim of this study was to analyse the resistance phenotype of OXA-423.

Methods: The strain WY-0713 was isolated from an intensive care unit patient. Read More

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November 2020

Carbapenem Resistant Carrying Isolated From Two Solid Organ Transplant Patients.

Front Cell Infect Microbiol 2020 26;10:563482. Epub 2020 Oct 26.

Department of Pathology and Laboratory Medicine, University of California, Los Angeles, Los Angeles, CA, United States.

resides in a variety of aquatic environments. Infections with mainly occur after contact with fresh or brackish water. Nosocomial infections with can also occur. Read More

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October 2020

Antibiogram and Genetic Characterization of Carbapenem-Resistant Gram-Negative Pathogens Incriminated in Healthcare-Associated Infections.

Infect Drug Resist 2020 4;13:3991-4002. Epub 2020 Nov 4.

Department of Bacteriology, Immunology and Mycology, Faculty of Veterinary Medicine, Suez Canal University, Ismailia 41522, Egypt.

Purpose: Carbapenems are considered the most efficient antibiotic used in the treatment of nosocomial infections. Carbapenem-resistant Gram-negative rods are becoming a serious hazard in hospitals threatening public health. The aim of the current study was to investigate the prevalence of carbapenem-resistant Gram-negative pathogens incriminated in healthcare-associated infections, along with antimicrobial resistance profiles, carbapenemase and metallo-β-lactamase production, and their molecular characterization. Read More

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November 2020

A Novel KPC Variant KPC-55 in ST307 of Reinforced Meropenem-Hydrolyzing Activity.

Front Microbiol 2020 7;11:561317. Epub 2020 Oct 7.

Department of Laboratory Medicine, Yonsei University College of Medicine, Seoul, South Korea.

A novel carbapenemase (KPC) variant, KPC-55, produced by a ST307 strain was characterized. strain BS407 was recovered from an active surveillance rectal swab of a patient newly admitted to a general hospital in Busan, South Korea. Carbapenemase production was confirmed by the modified Hodge test, and the MICs of β-lactams were determined by the broth microdilution method. Read More

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October 2020

Increasing Prevalence of and Producing CTX-M-Type Extended-Spectrum Beta-Lactamase, Carbapenemase, and NDM-1 in Patients from a Rural Community with Community Acquired Infections: A 3-Year Study.

Int J Appl Basic Med Res 2020 Jul-Sep;10(3):156-163. Epub 2020 Jul 11.

Department of Microbiology, SGT Medical College Hospital and Research Institute, Gurgaon, Haryana, India.

Background: Increasing prevalence of community-acquired infections (CAIs) due to and producing extended-spectrum beta-lactamase (ESBL), especially the Cefotaxime-Munich (CTX-M) type, carbapenemase, and New Delhi metallo-β-lactamase (NDM), has been reported globally posing a serious public health threat that has complicated treatment strategies for Gram-negative bacterial infections. While most of the reports in this regard are based on hospitalized patients from the urban community, there is a paucity of data in a rural community presenting with CAIs.

Materials And Methods: A total of 1275 strains of and isolated over a period of 3 years from patients with CAIs were subjected to the detection of ESBL by double-disc synergy test; carbapenemase by modified Hodge test; metallo-β-lactamase by MIC test strip metallo-β-lactamase (MBL); and , , , and genes by polymerase chain reaction. Read More

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Genetic Diversity of OXA-like Genes in Multidrug-Resistant Acinetobacter baumannii Strains from ICUs.

Clin Lab 2020 Oct;66(10)

Background: This study aimed to investigate the genetic diversity of OXA-51-like, OXA-23-like, OXA-24, and OXA-58-like genes and the role of β-lactamases in carbapenem resistance among multidrug resistant Acinetobacter baumannii strains recovered from patients in intensive care units (ICUs).

Methods: Non-duplicate clinical isolates of A. baumannii from ICUs that were identified as imipenem and meropenem resistant were collected. Read More

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October 2020

Combination of modified carbapenem inactivation method (mCIM) and EDTA-CIM (eCIM) for phenotypic detection of carbapenemase-producing Enterobacteriaceae.

BMC Microbiol 2020 10 17;20(1):315. Epub 2020 Oct 17.

Department of Clinical Laboratory, En Chu Kong Hospital, No. 399, Fuxing Rd., Sanxia Dist, New Taipei City, 23702, Taiwan.

Background: Carbapenemase-resistant Enterobacteriaceae (CRE) cause many serious infections resulting in increasing treatment cost, prolonged hospitalization, and mortality rate. Reduced expression and/or mutations of porins and the presence of carbapenemase promote Enterobacteriaceae survival under carbapenem treatments. Development of accurate methods for the detection of antimicrobial resistance is required not only for therapy but also to monitor the spread of resistant bacteria or resistance genes throughout the hospital and community. Read More

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October 2020

Metallo-Beta-Lactamase-Producing spp. from Clinical Isolates at a Tertiary Care Hospital in Ghana.

Biomed Res Int 2020 24;2020:3852419. Epub 2020 Sep 24.

Department of Medical Laboratory Science, School of Biomedical and Allied Health Sciences, College of Health Sciences, University of Ghana, Legon Accra, Ghana.

Metallo-beta-lactamase-producing spp. is a major challenge for therapeutic treatment of nosocomial infections. This study is aimed at determining the prevalence of MBL-producing spp. Read More

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September 2020

Emerge of NDM-1-Producing Multidrug-Resistant and Co-Harboring of Carbapenemase Genes in South of Iran.

Iran J Public Health 2020 May;49(5):959-967

Department of Bacteriology, Microbiology Research Center, Pasteur Institute of Iran, Tehran, Iran.

Background: New Delhi metallobetalactamase1 (NDM-1) is one of the most important emerging antibiotic resistance. Co-harboring three or four carbapenemases is rare and only a few reports exist in the literature. We described the characteristics of the large epidemic outbreaks and reports co-producing with the other carbapenemase genes in isolates. Read More

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Antibiogram, Prevalence of OXA Carbapenemase Encoding Genes, and RAPD-Genotyping of Multidrug-Resistant Incriminated in Hidden Community-Acquired Infections.

Antibiotics (Basel) 2020 Sep 15;9(9). Epub 2020 Sep 15.

Department of Medical Microbiology and Immunology, Faculty of Medicine, Assiut University, Assiut 71515, Egypt.

spp. has gained fame from their ability to resist difficult conditions and their constant development of antimicrobial resistance. This study aimed to investigate the prevalence, susceptibility testing, OXA carbapenemase-encoding genes, and RAPD-genotyping of multidrug resistant incriminated in hidden community-acquired infections in Egypt. Read More

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September 2020

Effect of Multilevel Upper Airway Surgery vs Medical Management on the Apnea-Hypopnea Index and Patient-Reported Daytime Sleepiness Among Patients With Moderate or Severe Obstructive Sleep Apnea: The SAMS Randomized Clinical Trial.

JAMA 2020 09;324(12):1168-1179

Adelaide Institute for Sleep Health, College of Medicine and Public Health, Flinders University, Adelaide, SA, Australia.

Importance: Many adults with obstructive sleep apnea (OSA) use device treatments inadequately and remain untreated.

Objective: To determine whether combined palatal and tongue surgery to enlarge or stabilize the upper airway is an effective treatment for patients with OSA when conventional device treatment failed.

Design, Setting, And Participants: Multicenter, parallel-group, open-label randomized clinical trial of upper airway surgery vs ongoing medical management. Read More

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September 2020

Detection of OXA-48 Gene in Carbapenem-Resistant and from Urine Samples.

Infect Drug Resist 2020 14;13:2311-2321. Epub 2020 Jul 14.

Central Department of Microbiology, Tribhuvan University, Kirtipur, Kathmandu, Nepal.

Introduction: Resistance to carbapenem in Gram-negative bacteria is attributable to their ability to produce carbapenemase enzymes. The main objective of this study was to detect the presence of genes in carbapenem-resistant uropathogenic and isolated from urine samples from patients attending Alka Hospital, Jawalakhel, Lalitpur, Nepal.

Methods: A total of 1013 mid-stream urine samples were collected from patients with suspected urinary tract infection (UTI) between April and September 2018. Read More

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High Mortality Rates Among Patients With Non-Traumatic Intracerebral Hemorrhage and Atrial Fibrillation on Antithrombotic Therapy Are Independent of the Presence of Cerebral Amyloid Angiopathy: Insights From a Population-Based Study.

J Am Heart Assoc 2020 08 25;9(15):e016893. Epub 2020 Jul 25.

Division of Cardiovascular Diseases Mayo Clinic Rochester MN.

Background Intracerebral hemorrhage (ICH) risk is higher in elderly patients with atrial fibrillation on antithrombotic therapy as well as those with cerebral amyloid angiopathy (CAA). We investigated if mortality among patients with atrial fibrillation on antithrombotic therapy presenting with non-traumatic ICH was influenced by underlying CAA. Methods and Results We used the Rochester Epidemiology Project to identify 6045 patients with atrial fibrillation aged >55 years on anticoagulation or antiplatelet therapy from 1995 to 2016. Read More

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Electronic cigarettes: A position statement from the Thoracic Society of Australia and New Zealand.

Respirology 2020 10 26;25(10):1082-1089. Epub 2020 Jul 26.

Department of Respiratory Medicine, Concord Repatriation General Hospital, Sydney, NSW, Australia.

The TSANZ develops position statements where insufficient data exist to write formal clinical guidelines. In 2018, the TSANZ addressed the question of potential benefits and health impacts of electronic cigarettes (EC). The working party included groups focused on health impacts, smoking cessation, youth issues and priority populations. Read More

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October 2020

Distribution of Class B and Class A β-Lactamases in Clinical Strains of : Comparison of Phenotypic Methods and High-Resolution Melting Analysis (HRMA) Assay.

Infect Drug Resist 2020 30;13:2037-2052. Epub 2020 Jun 30.

Microbiology Department, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.

Background: There are various phenotypic methods for identifying class B and class A β-lactamase enzymes in . The purpose of this study was to compare the sensitivity and specificity of different phenotypic methods with HRMA assay to detect β-lactamase-producing strains.

Methods: Eighty-eight of isolates were collected from different specimens. Read More

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Tumor control via targeting PD-L1 with chimeric antigen receptor modified NK cells.

Elife 2020 07 7;9. Epub 2020 Jul 7.

Translational Tumor Immunology Program, National Institute on Deafness and Other Communication Disorders, National Institutes of Health, Bethesda, United States.

Failed T cell-based immunotherapies in the presence of genomic alterations in antigen presentations pathways may be overcome by NK cell-based immunotherapy. This approach may still be limited by the presence of immunosuppressive myeloid populations. Here, we demonstrate that NK cells (haNKs) engineered to express a PD-L1 chimeric antigen receptor (CAR) haNKs killed a panel of human and murine head and neck cancer cells at low effector-to-target ratios in a PD-L1-dependent fashion. Read More

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Impact of dilute acid pretreatment conditions on p-coumarate removal in diverse maize lines.

Bioresour Technol 2020 Oct 28;314:123750. Epub 2020 Jun 28.

Department of Chemical & Biological Engineering, Montana State University, Bozeman, MT 59717, United States; Division of Sustainable Process Engineering, Luleå University of Technology, Luleå 97187, Sweden. Electronic address:

Prior work has identified that lignins recovered from dilute acid-pretreated corn stover exhibit superior performance in phenol-formaldehyde resins used in wood adhesive applications when compared to diverse process-modified lignins derived from other sources. This improved performance is hypothesized to be due to the higher content of unsubstituted phenolic groups specifically p-coumarate lignin esters. In this work, a diverse set of corn stover samples are employed that exhibit diversity in p-coumarate content and total lignin content to explore the relationship between dilute acid pretreatment conditions, p-coumarate ester hydrolysis, xylan solubilization, and the resulting glucose enzymatic hydrolysis yields. Read More

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October 2020

Use of the CUSUM and Shewhart control chart methods to identify changes of public health significance using childhood blood lead surveillance data.

Environ Epidemiol 2020 Apr;4(2)

Division of Environmental Health Science and Practice, National Center for Environmental Health, Centers for Disease Control and Prevention, Atlanta, Georgia.

Background: Local, state, and national childhood blood lead surveillance is based on healthcare providers and clinical laboratories reporting test results to public health departments. Increased interest in detecting blood lead level (BLL) patterns and changes of potential public health significance in a timely manner has highlighted the need for surveillance systems to rapidly detect and investigate these events.

Objective: Decrease the time to detect changes in surveillance patterns by using an alerting algorithm developed and assessed through historical child blood lead surveillance data analysis. Read More

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Genetic Characterization of Carbapenemase-Producing Complex and of Food of Animal Origin from Egypt.

Microb Drug Resist 2021 Feb 26;27(2):196-203. Epub 2020 Jun 26.

Laboratory of Food Microbiology and Hygiene, Graduate School of Integrated Sciences for Life, Hiroshima University, Higashi-Hiroshima, Japan.

The increasing spread of carbapenem resistance is a serious global public health concern that negatively affects human and animal health. In this study we characterized the carbapenemase production in gram-negative bacteria isolated from different meat and meat products in Egypt. Phenotypic and genotypic susceptibility testing were investigated. Read More

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February 2021

Molecular Epidemiology of Carbapenemase-Producing Isolated from an Iranian University Hospital: Evidence for Spread of High-Risk Clones.

Infect Drug Resist 2020 3;13:1583-1592. Epub 2020 Jun 3.

Uro-Oncology Research Center, Tehran University of Medical Sciences, Tehran, Iran.

Purpose: Given the importance of treatment failure due to multidrug-resistant (MDR) strains, studies on population structure of these organisms are necessary to improve control strategies. Accordingly, the current study aimed to determine the prevalence of carbapenem-resistant (CRPA) at a teaching referral hospital in Iran and to analyz their molecular clonality by multilocus sequence typing (MLST) and pulsed-field gel electrophoresis (PFGE) for epidemiological purposes.

Methods: In this study, modified Hodge test (MHT) and double-disk synergy test (DDST) were used for carbapenemase production and metallo-β-lactamases (MBLs) screening, respectively. Read More

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Subarachnoid Hemorrhage "Fast Track": A Health Economics and Health Care Redesign Approach for Early Selected Hospital Discharge.

Mayo Clin Proc Innov Qual Outcomes 2020 Jun 5;4(3):238-248. Epub 2020 Jun 5.

Department of Neurologic Surgery, Mayo Clinic, Jacksonville, FL.

Objective: To determine whether earlier hospital discharge is feasible and safe in selected patients with subarachnoid hemorrhage (SAH) using an outpatient "fast-track" protocol.

Patients And Methods: We conducted a prospective quality improvement cohort study with the primary feasibility end point of patients with SAH deemed safe for discharge by treating team consensus. All patients received detailed education and outpatient transcranial Doppler monitoring; caregivers could contact the on-call team 24-7. Read More

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Phenotypic and genotypic detection of metallo-beta-lactamases in Carbapenem resistant Acinetobacter baumannii.

Caspian J Intern Med 2020 ;11(2):171-176

Infectious Diseases and Tropical Medicine Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran.

Background: Carbapenem resistance in has become a major concern for treating physicians. The aim of this study was to investigate the prevalence of metallo β-lactamase (MBL) genes () among isolated multidrug-resistant .

Methods: Fifty non-repetitive carbapenem-resistant isolates were collected. Read More

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January 2020