Nucleic Acids Res 2014 Feb 30;42(4):2602-23. Epub 2013 Nov 30.
Laboratory of RNA Modification and Mitochondrial Diseases, Príncipe Felipe Research Center, 46012-Valencia, Spain, Department of Molecular Biology, Umeå University, S90187, Sweden and Biomedical Research Networking Centre for Rare Diseases (CIBERER) (node U721), Spain.
In Escherichia coli, the MnmEG complex modifies transfer RNAs (tRNAs) decoding NNA/NNG codons. MnmEG catalyzes two different modification reactions, which add an aminomethyl (nm) or carboxymethylaminomethyl (cmnm) group to position 5 of the anticodon wobble uridine using ammonium or glycine, respectively. In tRNA(cmnm5s2UUG)(Gln) and tRNA(cmnm5UmAA)(Leu), however, cmnm(5) appears as the final modification, whereas in the remaining tRNAs, the MnmEG products are converted into 5-methylaminomethyl (mnm(5)) through the two-domain, bi-functional enzyme MnmC. Read More