817 results match your criteria mms sensitivity

Elevated DNA damage sensitivity in malaria parasite induced by the synergistic action between PfHsp90 inhibitor and PfRad51 inhibitor.

Antimicrob Agents Chemother 2021 Jun 7:AAC0045721. Epub 2021 Jun 7.

Department of Biochemistry, School of Life Sciences, University of Hyderabad, India.

The DNA recombinase Rad51 from human malaria parasite has emerged as a potential drug target due to its central role in the homologous recombination (HR) mediated double strand break (DSB) repair pathway. Inhibition of the ATPase and strand exchange activity of PfRad51, by a small molecule inhibitor B02 (3-(Phenylmethyl)-2-[(1E)-2-(3-pyridinyl)ethenyl]-4(3H)-quinazolinone), renders the parasite more sensitive towards the genotoxic agents. Here, we investigated whether the inhibition of the molecular chaperone PfHsp90 potentiates the anti-malarial action of B02. Read More

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Evaluation of the MICRONAUT MIC-strip colistin assay for colistin susceptibility testing of carbapenem-resistant Acinetobacter baumannii and Enterobacterales.

Diagn Microbiol Infect Dis 2021 Apr 16;100(4):115391. Epub 2021 Apr 16.

National Institute for Antibiotic Resistance and Infection Control, Ministry of Health, Israel. Electronic address:

We sought to evaluate the MICRONAUT MIC-Strip colistin (MMS) assay, a commercial broth microdilution (BMD) panel, using 273 carbapenem-resistant Acinetobacter baumannii and Enterobacterales isolates. BMD was used as gold standard. MMS had 98. Read More

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Yeast-based screening of cancer mutations in the DNA damage response protein Mre11 demonstrates importance of conserved capping domain residues.

Mol Biol Rep 2021 Jun 1. Epub 2021 Jun 1.

Department of Biological and Environmental Sciences, Longwood University, Farmville, VA, 23901, USA.

DNA damage response (DDR) pathways are initiated to prevent mutations from being passed on in the event of DNA damage. Mutations in DDR proteins can contribute to the development and maintenance of cancer cells, but many mutations observed in human tumors have not been functionally characterized. Because a proper response to DNA damage is fundamental to living organisms, DDR proteins and processes are often highly conserved. Read More

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Vibro-Thermal Wave Radar: Application of Barker Coded Amplitude Modulation for Enhanced Low-Power Vibrothermographic Inspection of Composites.

Materials (Basel) 2021 May 7;14(9). Epub 2021 May 7.

Mechanics of Materials and Structures (UGent-MMS), Department of Materials, Textiles and Chemical Engineering (MaTCh), Ghent University, Technologiepark-Zwijnaarde 46, 9052 Zwijnaarde, Belgium.

This paper proposes an efficient non-destructive testing technique for composite materials. The proposed vibro-thermal wave radar (VTWR) technique couples the thermal wave radar imaging approach to low-power vibrothermography. The VTWR is implemented by means of a binary phase modulation of the vibrational excitation, using a 5 bit Barker coded waveform, followed by matched filtering of the thermal response. Read More

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Leukocyte cell population data from the blood cell analyzer as a predictive marker for severity of acute pancreatitis.

J Clin Lab Anal 2021 Jun 1:e23863. Epub 2021 Jun 1.

Department of Emergency, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Background: The prediction for severe acute pancreatitis (SAP) is the key to give timely targeted treatment. Leukocyte cell population data (CPD) have been widely applied in early prediction and diagnosis of many diseases, but their predictive ability for SAP remains unexplored. We aim to testify whether CPD could be an indicator of AP severity in the early stage of the disease. Read More

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Correlation Between Choriocapillaris Density and Retinal Sensitivity in Age-Related Macular Degeneration.

Transl Vis Sci Technol 2021 Jun;10(7)

Eye Clinic, Multidisciplinary Department of Medical, Surgical and Dental Sciences, University of Campania Luigi Vanvitelli, Via S. Pansini 5, Naples, Italy.

Purpose: The purpose of this study was to investigate the relationship between perfusion of the choriocapillaris (CC) and retinal sensitivity in eyes with intermediate age-related macular degeneration (iAMD).

Methods: This prospective study included patients with iAMD and healthy controls. All enrolled subjects underwent optical coherence tomography angiography (OCT-A) in order to compute the percent perfused choriocapillaris area (PPCA). Read More

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Division of labor of Y-family polymerases in translesion-DNA synthesis for distinct types of DNA damage.

PLoS One 2021 1;16(6):e0252587. Epub 2021 Jun 1.

Department of Chemistry, Graduate School of Science, Tokyo Metropolitan University, Hachioji-shi, Tokyo, Japan.

Living organisms are continuously under threat from a vast array of DNA-damaging agents, which impact genome DNA. DNA replication machinery stalls at damaged template DNA. The stalled replication fork is restarted via bypass replication by translesion DNA-synthesis polymerases, including the Y-family polymerases Polη, Polι, and Polκ, which possess the ability to incorporate nucleotides opposite the damaged template. Read More

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Characteristics of fixation patterns and their relationship with visual function of patients with idiopathic macular holes after vitrectomy.

Sci Rep 2021 Apr 7;11(1):7658. Epub 2021 Apr 7.

Tianjin Eye Hospital, Tianjin Key Lab of Ophthalmology and Visual Science, Tianjin Eye Institute, Nankai University affiliated Eye Hospital, Clinical College of Ophthalmology, Tianjin Medical University, Gansu Road 4, Heping District, Tianjin, 300020, China.

To analyze the relationships between the fixation location and the visual function of idiopathic macular hole (IMH) patients with macular integrity assessment (MAIA) examination preoperatively and 3 months postoperatively. This was a retrospective case analysis. Forty-three eyes of 43 patients diagnosed with IMH were included in this study. Read More

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Diagnostic accuracy of brief cognitive screening tools to diagnose vascular cognitive impairment in Peru.

Int J Geriatr Psychiatry 2021 Mar 8. Epub 2021 Mar 8.

Servicio de Neurología, Instituto Peruano de Neurociencias, Lima, Perú.

Introduction: To evaluate the diagnostic accuracy of three brief cognitive screening (BCS) tools, Peruvian version of Addenbrooke's Cognitive Examination (ACE-Pe), of INECO Frontal Screening (IFS-Pe) and of the Mini-Mental State Examination (MMSE-Pe), for the diagnosis of vascular cognitive impairment (VCI) and its non-dementia stages (VCI-ND) and vascular dementia (VD) in patients with cerebral stroke in Lima-Peru.

Materials And Methods: A cohort analysis to evaluate the diagnostic accuracy of three BCS for VCI.

Results: Two hundred and four patients were evaluated: 61% Non-VCI, 30% VCI-ND and 9% VD. Read More

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Homolog of Saccharomyces cerevisiae SLX4 is required for cell recovery from MMS-induced DNA damage in Candida albicans.

FEMS Yeast Res 2021 Mar;21(2)

School of Life Sciences, Beijing University of Chinese Medicine, Beijing 102488, China.

SLX4 is a scaffold to coordinate the action of structure-specific endonucleases that are required for homologous recombination and DNA repair. In view of ScSLX4 functions in the maintenance and stability of the genome in Saccharomyces cerevisiae, we have explored the roles of CaSLX4 in Candida albicans. Here, we constructed slx4Δ/Δ mutant and found that it exhibited increased sensitivity to the DNA damaging agent, methyl methanesulfonate (MMS) but not the DNA replication inhibitor, hydroxyurea (HU). Read More

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Validation of the Lyon classification for GORD diagnosis: acid exposure time assessed by prolonged wireless pH monitoring in healthy controls and patients with erosive oesophagitis.

Gut 2021 Feb 12. Epub 2021 Feb 12.

Oesophageal Physiology Laboratory, Guy's and St Thomas' NHS Foundation Trust, London, UK

Objective: Acid exposure time (AET) from ambulatory pH studies and reflux oesophagitis are independent measurements used by the Lyon classification to diagnose GORD. This study aimed to validate AET reference ranges and diagnostic thresholds by analysis of 96-hour wireless pH studies from healthy, asymptomatic controls (HCs) and patients with and without oesophagitis.

Design: HC and consecutive patients referred for wireless pH studies (off acid suppressants for >7 days) underwent 96-hour pH studies at two tertiary referral centres. Read More

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February 2021

Computed tomographic angiography may be used for assessing the dilatation of the anterior choroidal and posterior communicating arteries in patients with moyamoya syndrome.

Eur Radiol 2021 Feb 9. Epub 2021 Feb 9.

Department of Radiology, the Affiliated Hospital of Jining Medical University, Jining, China.

Objective: To evaluate the feasibility of CT angiography (CTA) for assessing anterior choroidal artery (AChA) and posterior communicating artery (PComA) dilatation in patients with moyamoya syndrome (MMS).

Methods: Eighty-eight MMS patients who underwent digital subtraction angiography (DSA) and CTA within 1 month were enrolled. The AChA was graded using both DSA and CTA. Read More

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February 2021

Kleisin NSE4 of the SMC5/6 complex is necessary for DNA double strand break repair, but not for recovery from DNA damage in Physcomitrella (Physcomitrium patens).

Plant Mol Biol 2021 Feb 6. Epub 2021 Feb 6.

Institute of Experimental Botany, The Czech Academy of Sciences, Na Karlovce 1, 16000, Prague, Czech Republic.

Key Message: Kleisin NSE4 and circular form of SMC5/6 is indispensable for DSB repair and necessary for gene targeting but is not enough for recovery of cells from DNA damage in Physcomitrella. Structural maintenance of chromosomes (SMC) complexes are involved in cohesion, condensation and maintenance of genome stability. Based on the sensitivity of mutants to genotoxic stress the SMC5/6 complex is thought to play a prominent role in DNA stabilization during repair by tethering DNA at the site of lesion by a heteroduplex of SMC5 and SMC6 encircled with non-SMC components NSE1, NSE3 and kleisin NSE4. Read More

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February 2021

A Conserved Histone H3-H4 Interface Regulates DNA Damage Tolerance and Homologous Recombination during the Recovery from Replication Stress.

Mol Cell Biol 2021 03 24;41(4). Epub 2021 Mar 24.

Department of Molecular Biology, Graduate School of Science, Gakushuin University, Tokyo, Japan

In eukaryotes, genomic DNA is packaged into nucleosomes, which are the basal components coordinating both the structures and functions of chromatin. In this study, we screened a collection of mutations for histone H3/H4 mutants in that affect the DNA damage sensitivity of DNA damage tolerance (DDT)-deficient cells. We identified a class of histone H3/H4 mutations that suppress methyl methanesulfonate (MMS) sensitivity of DDT-deficient cells (referred to here as the histone SDD mutations), which likely cluster on a specific H3-H4 interface of the nucleosomes. Read More

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Measurement and monitoring patient safety in prehospital care: a systematic review.

Int J Qual Health Care 2021 Feb;33(1)

Irish Centre for Applied Patient Safety and Simulation, National University of Ireland Galway, Galway H91 TK33, County Galway, Ireland.

Background: Prehospital care is potentially hazardous with the possibility for patients to experience an adverse event. However, as compared to secondary care, little is known about how patient safety is managed in prehospital care settings.

Objectives: The objectives of this systematic review were to identify and classify the methods of measuring and monitoring patient safety that have been used in prehospital care using the five dimensions of the Measuring and Monitoring Safety (MMS) framework and use this classification to identify where there are safety 'blind spots' and make recommendations for how these deficits could be addressed. Read More

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February 2021

Stable isotope labeling derivatization combined with multiple-mass spectrometry technologies to monitor metabolites of tenuifoliside A incubated with intestinal bacteria incubation model.

Talanta 2021 Mar 19;224:121791. Epub 2020 Oct 19.

National Center of Mass Spectrometry in Changchun and Jilin Provincial Key Laboratory of Chinese Medicine Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, China. Electronic address:

Aromatic carboxylic acids (ACAs), play important roles in preventive and therapeutic effects for some diseases. However, complex matrix effect and poor detection sensitivity make it difficult and even rare to detect ACAs in complex bio-samples. Herein, a stable isotope labeling derivatization (SILD) method based on one-pot synthesis of carboxylic amides by aniline (AN) and aniline-d (AN-d) was firstly designed for quantitatively monitoring ACAs under mild conditions. Read More

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Transposition and Normalization of the Mini-Mental State Examination in French Sign Language.

Arch Clin Neuropsychol 2020 Dec 30. Epub 2020 Dec 30.

Réseau Sourds et Santé, Groupement des Hôpitaux de l'Institut Catholique de Lille, Lille F-59000, France.

Objective: Due to the lack of a validated translation, the mini-mental state examination (MMSE) cannot be used to screen for suspected dementia in deaf people who communicate in French Sign Language (FSL). Taking into consideration the cultural and linguistic features of this specific population, we transposed the validated French version of the MMSE into a version adapted to FSL users: mini-mental state-langue des signes (MMS-LS). The objective of our work was to obtain screening norms for the MMS-LS. Read More

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December 2020

Oxidation resistance 1 functions in the maintenance of cellular survival and genome stability in response to oxidative stress-independent DNA damage.

Genes Environ 2020 Nov 10;42(1):29. Epub 2020 Nov 10.

Laboratory of Stress Response Biology, Department of Zoology, Division of Biological Sciences, Graduate School of Science, Kyoto University, Kitashirakawa Oiwake-cho, Sakyo-ku, Kyoto, 606-8502, Japan.

Background: DNA damage is generated by various intrinsic and extrinsic sources such as reactive oxygen species (ROS) and environmental mutagens, and causes genomic alterations. DNA damage response (DDR) is activated to induce cell cycle arrest and DNA repair. Oxidation resistance 1 (OXR1) is a protein that defends cells against oxidative stress. Read More

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November 2020

Pre-examinations Improve Automated Metastases Detection on Cranial MRI.

Invest Radiol 2021 May;56(5):320-327

Department for Medical Image Computing, German Cancer Research Center, Heidelberg, Germany.

Materials And Methods: Our local ethics committee approved this retrospective monocenter study.First, a dual-time approach was assessed, for which the CNN was provided sequences of the MRI that initially depicted new MM (diagnosis MRI) as well as of a prediagnosis MRI: inclusion of only contrast-enhanced T1-weighted images (CNNdual_ce) was compared with inclusion of also the native T1-weighted images, T2-weighted images, and FLAIR sequences of both time points (CNNdual_all).Second, results were compared with the corresponding single time approaches, in which the CNN was provided exclusively the respective sequences of the diagnosis MRI. Read More

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Yeast Rpn4 Links the Proteasome and DNA Repair via Regulation.

Int J Mol Sci 2020 Oct 30;21(21). Epub 2020 Oct 30.

Center for Precision Genome Editing and Genetic Technologies for Biomedicine, Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Vavilov str. 32, 119991 Moscow, Russia.

Environmental and intracellular factors often damage DNA, but multiple DNA repair pathways maintain genome integrity. In yeast, the 26S proteasome and its transcriptional regulator and substrate Rpn4 are involved in DNA damage resistance. Paradoxically, while proteasome dysfunction may induce hyper-resistance to DNA-damaging agents, Rpn4 malfunction sensitizes yeasts to these agents. Read More

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October 2020

One-step quantification of salivary exosomes based on combined aptamer recognition and quantum dot signal amplification.

Biosens Bioelectron 2021 Jan 14;171:112733. Epub 2020 Oct 14.

The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) and Key Laboratory of Oral Biomedicine Ministry of Education, School and Hospital of Stomatology, Wuhan University, Wuhan, 430079, PR China. Electronic address:

As promising fluid biomarkers for non-invasive diagnosis, naturally-occurring exosomes in saliva have attracted a wide interest for their potential application in oral diseases especially oral cancers. However, accurate quantification of salivary exosomes is still challenging due to the current difficulties in simultaneous identification and measurement of these nano-sized vesicles. In this study, we developed a novel fluorescent biosensor for one-step sensitive quantification of salivary exosomes based on magnetic and fluorescent bio-probes (MFBPs). Read More

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January 2021

Proteome analysis of after methyl methane sulfonate (MMS) treatment.

Biochem Biophys Rep 2020 Dec 3;24:100820. Epub 2020 Oct 3.

Molecular and Structural Biology Division, CSIR-CDRI, Sector 10, Jankipuram Extension, Lucknow, PIN 226 031, India.

The treatment of methyl methane sulfonate (MMS) increases sensitivity to the DNA damage which, further leads to the cell death followed by a cell cycle delay. Delay in the cell cycle is because of the change in global transcription regulation which results into proteome change. There are several microarray studies on the transcriptome changes after MMS treatment, but very few studies are reported related to proteome change. Read More

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December 2020

Diagnostic test assessment. Validation study of an alternative system to detect microsatellite instability in colorectal carcinoma.

Pathologica 2020 Dec 28;112(4):178-183. Epub 2020 Jul 28.

Department of Oncology, Ospedali Riuniti Villa Sofia-Cervello, Palermo, Italy.

The American Society for Clinical Pathology (ASCP), College of American Pathologists (CAP), Association for Molecular Pathology (AMP), and the American Society of Clinical Oncology (ASCO) have been recently strongly recommended the evaluation of mismatch repair status (MMS) as molecular biomarkers in colorectal cancer for a better prognostic stratification of patients. This recommendation is emphasized by the recent evidence of Microsatellite Instability (MSI) as a predictive marker for chemotherapy and immunotherapy.

In this scenario, the validation of molecular biomarker testing methods seems to be essential to design the most appropriate tailored therapy and the most suitable care strategy, respectively. Read More

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December 2020

Babesia bovis Rad51 ortholog influences switching of ves genes but is not essential for segmental gene conversion in antigenic variation.

PLoS Pathog 2020 08 31;16(8):e1008772. Epub 2020 Aug 31.

Department of Infectious Diseases and Immunology, University of Florida, Gainesville, Florida, United States of America.

The tick-borne apicomplexan parasite, Babesia bovis, a highly persistent bovine pathogen, expresses VESA1 proteins on the infected erythrocyte surface to mediate cytoadhesion. The cytoadhesion ligand, VESA1, which protects the parasite from splenic passage, is itself protected from a host immune response by rapid antigenic variation. B. Read More

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Magnetic Micro Sensors with Two Magnetic Field Effect Transistors Fabricated Using the Commercial Complementary Metal Oxide Semiconductor Process.

Sensors (Basel) 2020 Aug 21;20(17). Epub 2020 Aug 21.

Department of Mechanical Engineering, National Chung Hsing University, Taichung 402, Taiwan.

The fabrication and characterization of a magnetic micro sensor (MMS) with two magnetic field effect transistors (MAGFETs) based on the commercial complementary metal oxide semiconductor (CMOS) process are investigated. The magnetic micro sensor is a three-axis sensing type. The structure of the magnetic microsensor is composed of an x/y-MAGFET and a z-MAGFET. Read More

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Automated, High-Throughput Infrared Spectroscopy for Secondary Structure Analysis of Protein Biopharmaceuticals.

J Pharm Sci 2020 10 3;109(10):3223-3230. Epub 2020 Aug 3.

Pfizer, Inc., BioTherapeutics Pharmaceutical Sciences, Analytical Research and Development, Andover, MA 01810, USA. Electronic address:

Protein higher order structure (HOS) is an important product quality attribute that governs the structure-function characteristics, safety, and efficacy of therapeutic proteins. Infrared (IR) spectroscopy has long been recognized as a powerful biophysical tool in determining protein secondary structure and monitoring the dynamic structural changes. Such biophysics analyses help establish process and product knowledge, understand the impact of upstream (cell culture) and downstream (purification) process conditions, create stable formulations, monitor product stability, and assess product comparability when process improvements are implemented (or establish biosimilarity to originator products). Read More

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October 2020

Identification of APTX disease-causing mutation in two unrelated Jordanian families with cerebellar ataxia and sensitivity to DNA damaging agents.

PLoS One 2020 4;15(8):e0236808. Epub 2020 Aug 4.

Cell Therapy Center, The University of Jordan, Amman, Jordan.

Background: Ataxia with oculomotor apraxia type 1 (AOA1) is a rare autosomal recessive cerebellar ataxia, caused by mutations in the APTX gene. The disease is characterized by early-onset cerebellar ataxia, oculomotor apraxia and severe axonal polyneuropathy. The aim of this study was to detect the disease-causing variants in two unrelated consanguineous Jordanian families with cerebellar ataxia using whole exome sequencing (WES), and to correlate the identified mutation(s) with the clinical and cellular phenotypes. Read More

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October 2020

Toxicity to RAW264.7 Macrophages of Silica Nanoparticles and the E551 Food Additive, in Combination with Genotoxic Agents.

Nanomaterials (Basel) 2020 Jul 21;10(7). Epub 2020 Jul 21.

Université Grenoble-Alpes, CEA, CNRS, IRIG-DIESE, SyMMES, Chemistry Interface Biology for the Environment, Health and Toxicology (CIBEST), F-38000 Grenoble, France.

Synthetic amorphous silica (SAS) is used in a plethora of applications and included in many daily products to which humans are exposed via inhalation, ingestion, or skin contact. This poses the question of their potential toxicity, particularly towards macrophages, which show specific sensitivity to this material. SAS represents an ideal candidate for the adsorption of environmental contaminants due to its large surface area and could consequently modulate their toxicity. Read More

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Evaluation of Prognostic Factors of Severity in Acute Biliary Pancreatitis.

Int J Mol Sci 2020 Jun 16;21(12). Epub 2020 Jun 16.

Biophysics Institute, Faculty of Medicine, University of Coimbra, 3000-548 Coimbra, Portugal.

Acute pancreatitis (AP) is an inflammatory disorder of the pancreas that, when classified as severe, is associated with high morbidity and mortality. Promptly identifying the severity of AP is of extreme importance for improving clinical outcomes. The aim of this study was to compare the prognostic value of serological biomarkers, ratios, and multifactorial scores in patients with acute biliary pancreatitis and to identify the best predictors. Read More

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Correlation of MMP-9 and HMGB1 expression with the cognitive function in patients with epilepsy and factors affecting the prognosis.

Cell Mol Biol (Noisy-le-grand) 2020 Jun 8;66(3):39-47. Epub 2020 Jun 8.

Department of Neurosurgery, Jiaxiang People's Hospital, Jining 272400, China.

This study was designed to investigate the expressions and roles of MMP-9 and HMGB1 in peripheral blood of patients with epilepsy and their relationship with the cognitive function and to explore factors affecting the prognosis of epilepsy patients. A total of 127 patients with epilepsy were collected in the study group and 120 healthy subjects receiving a physical examination at the same time were collected in the control group. The MMP-9 and HMGB1 expressions and their diagnostic value for epilepsy were compared between the two groups. Read More

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