77,673 results match your criteria mitochondrial membrane


Photodiagnosis Photodyn Ther 2021 Jun 20:102403. Epub 2021 Jun 20.

Photobiology Applied to Health - Universidade do Vale do Paraíba. Av. Shishima Hifumi, 2911, Urbanova, São José dos Campos, S.P, Brazil. Electronic address:

Leishmaniasis is a zoonotic disease, regarded by WHO as a public health problem that has presented a significant increase in the recent years. Conventional treatment is toxic and leads to serious side effects. Photodynamic therapy has been studied as an alternative treatment to cutaneous leishmaniasis. Read More

View Article and Full-Text PDF

TAK1 inhibition elicits mitochondrial ROS to block intracellular bacterial colonization.

Proc Natl Acad Sci U S A 2021 Jun;118(25)

Department of Biological Sciences, North Carolina State University, Raleigh, NC 27695-7633;

Mitogen-activated protein kinase kinase kinase 7 (MAP3K7), known as TAK1, is an intracellular signaling intermediate of inflammatory responses. However, a series of mouse gene deletion analyses have revealed that ablation of TAK1 does not prevent but rather elicits inflammation, which is accompanied by elevation of reactive oxygen species (ROS). This has been considered a consequence of impaired TAK1-dependent maintenance of tissue integrity. Read More

View Article and Full-Text PDF

Loss of vacuolar type H+-ATPase induces caspase-independent necrosis-like death of hair cells in zebrafish neuromast.

Dis Model Mech 2021 Jun 23. Epub 2021 Jun 23.

Department of Cell and Developmental Biology, State University of New York Upstate Medical University, Syracuse, New York, USA.

The vacuolar type H+-ATPase (V-ATPase) is a ubiquitous membrane-bound, multi-subunit proton pump that regulates pH of cellular compartments. V-ATPase activity is known to modulate several cellular processes, but cell type-specific V-ATPase functions remain poorly understood. Patients with mutations in specific V-ATPase subunits can develop sensorineural deafness, but underlying mechanisms are unclear. Read More

View Article and Full-Text PDF

CRISPR/Cas9-engineered Drosophila knock-in models to study VCP diseases.

Dis Model Mech 2021 Jun 23. Epub 2021 Jun 23.

Louisiana State University, Department of Biological Sciences, Baton Rouge, LA 70803, USA.

Valosin containing protein (VCP) is a hexameric type II AAA ATPase required for several cellular processes including ER-associated degradation, organelle biogenesis, autophagy and membrane fusion. VCP contains three domains: a regulatory N-terminal domain and two ATPase domains (D1 and D2). Mutations in the N-terminal and D1 domains are associated with several degenerative diseases, including Multisystem Proteinopathy (MSP-1) and ALS. Read More

View Article and Full-Text PDF

Comparative Lipidomics of Chick Yolk Sac during the Embryogenesis Provides Insight into Understanding the Development-Related Lipid Supply.

J Agric Food Chem 2021 Jun 23. Epub 2021 Jun 23.

CASS Food Research Centre, School of Exercise and Nutrition Sciences, Deakin University, Burwood, Victoria 3125, Australia.

Yolk sac (YS, include the yolk content) at different chick embryogenesis stages possesses varying lipid distributions, which are nutrition-influencing factors for the health of an early embryo and a later adult. YS lipids can substantially influence embryogenesis metabolism, but a comprehensive understanding of lipid's influence remains unknown. Herein, the effects of embryogenesis on lipid profiling of chick YS were investigated by UHPLC-MS/MS-based lipidomics. Read More

View Article and Full-Text PDF

Protective effect of purple corn silk extract against ultraviolet-B-induced cell damage in human keratinocyte cells.

J Adv Pharm Technol Res 2021 Apr-Jun;12(2):140-146. Epub 2021 Apr 27.

Department of Biochemistry, Faculty of Medicine, Srinakharinwirot University, Bangkok, Thailand.

Ultraviolet-B (UVB) could lead to inflammation and cell death induction. Purple corn silk (PCS), part of female flower of corn has multiple pharmacological properties. This investigation focused on determining the preventive effects of PCS extract on human keratinocyte HaCaT cell damage induced by UVB irradiation. Read More

View Article and Full-Text PDF

Mn-rich oxide/persistent luminescence nanoparticles achieve light-free generation of singlet oxygen and hydroxyl radicals for responsive imaging and tumor treatment.

Theranostics 2021 25;11(15):7439-7449. Epub 2021 May 25.

State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics & Center for Molecular Imaging and Translational Medicine, School of Public Health, Xiamen University, Xiamen 361102, China.

X-ray excited persistent luminescence (XEPL) imaging has attracted increasing attention in biomedical imaging due to elimination of autofluorescence, high signal-to-noise ratio and repeatable activation with high penetration. However, optical imaging still suffers from limited for high spatial resolution. Herein, we report Mn-rich manganese oxide (MnO)-coated chromium-doped zinc gallogermanate (ZGGO) nanoparticles (Mn-ZGGOs). Read More

View Article and Full-Text PDF

Treatment with HIV-protease inhibitor nelfinavir identifies membrane lipid composition and fluidity as a therapeutic target in advanced multiple myeloma.

Cancer Res 2021 Jun 22. Epub 2021 Jun 22.

Experimental Oncology and Hematology, Department of Oncology and Hematology, Kantonsspital St. Gallen.

The HIV-protease inhibitor nelfinavir has shown broad anti-cancer activity in various preclinical and clinical contexts. In patients with advanced, proteasome inhibitor (PI)-refractory multiple myeloma (MM), nelfinavir-based therapy resulted in 65% partial response or better, suggesting that this may be a highly active chemotherapeutic option in this setting. The broad anti-cancer mechanism of action of nelfinavir implies that it interferes with fundamental aspects of cancer cell biology. Read More

View Article and Full-Text PDF

Mitochondrial metabolism regulates macrophage biology.

J Biol Chem 2021 Jun 19:100904. Epub 2021 Jun 19.

Affiliation: School of Life Science and Technology, ShanghaiTech University, Shanghai, China. Electronic address:

Mitochondria are critical for regulation of the activation, differentiation, and survival of macrophages and other immune cells. In response to various extracellular signals, such as microbial or viral infection, changes to mitochondrial metabolism and physiology could underlie the corresponding state of macrophage activation. These changes include alterations of oxidative metabolism, mitochondrial membrane potential, and tricarboxylic acid (TCA) cycling, as well as the release of mitochondrial reactive oxygen species (mtROS) and mitochondrial DNA (mtDNA) and transformation of the mitochondrial ultrastructure. Read More

View Article and Full-Text PDF

Light-inducible deformation of mitochondria in live cells.

Cell Chem Biol 2021 Jun 8. Epub 2021 Jun 8.

Department of Biomedical Engineering, The Chinese University of Hong Kong, Sha Tin, Hong Kong SAR, China; Shun Hing Institute of Advanced Engineering (SHIAE), The Chinese University of Hong Kong, Sha Tin, Hong Kong SAR, China. Electronic address:

Mitochondria, the powerhouse of the cell, are dynamic organelles that undergo constant morphological changes. Increasing evidence indicates that mitochondria morphologies and functions can be modulated by mechanical cues. However, the mechano-sensing and -responding properties of mitochondria and the relation between mitochondrial morphologies and functions are unclear due to the lack of methods to precisely exert mechano-stimulation on and deform mitochondria inside live cells. Read More

View Article and Full-Text PDF

N-acetylcysteine ameliorate cytotoxic injury in piglets sertoli cells induced by zearalenone and deoxynivalenol.

Environ Sci Pollut Res Int 2021 Jun 22. Epub 2021 Jun 22.

College of Animal Science and Technology, Anhui Agricultural University, 130 West Changjiang Road, Hefei, 230036, China.

Zearalenone (ZEA) and Deoxynivalenol (DON) are two mycotoxins highly detected in agricultural products and feed. Both mycotoxins produce reproductive toxicity and pose a serious threat to human and animal health, among which pigs are the most sensitive animals. Sertoli cells (SCs) play an important role in spermatogenesis; however, the combined toxicity of ZEA and DON and the screening of effective protective agents remains to be determined. Read More

View Article and Full-Text PDF

Apoptotic mechanisms of myricitrin isolated from Madhuca longifolia leaves in HL-60 leukemia cells.

Mol Biol Rep 2021 Jun 22. Epub 2021 Jun 22.

Medicinal Chemistry and Immunology Lab (ASK-II-406), School of Chemical and Biotechnology (SCBT), SASTRA Deemed University, Thanjavur, India.

Myricitrin, a naturally occurring flavonoid in Madhuca longifolia, possesses several medicinal properties. Even though our earlier work revealed its role against the proliferation of acute myelogenous leukemia cells (HL-60), its molecular mechanisms have not yet been revealed. The current study aims to explore the molecular mechanisms of myricitrin (isolated from an ethnomedicinal drug Madhuca longifolia) to induce apoptosis in HL-60 cells. Read More

View Article and Full-Text PDF

GSAP regulates lipid homeostasis and mitochondrial function associated with Alzheimer's disease.

J Exp Med 2021 Aug 22;218(8). Epub 2021 Jun 22.

Laboratory of Molecular and Cellular Neuroscience, The Rockefeller University, New York, NY.

Biochemical, pathogenic, and human genetic data confirm that GSAP (γ-secretase activating protein), a selective γ-secretase modulatory protein, plays important roles in Alzheimer's disease (AD) and Down's syndrome. However, the molecular mechanism(s) underlying GSAP-dependent pathogenesis remains largely elusive. Here, through unbiased proteomics and single-nuclei RNAseq, we identified that GSAP regulates multiple biological pathways, including protein phosphorylation, trafficking, lipid metabolism, and mitochondrial function. Read More

View Article and Full-Text PDF

Biogenic selenium nanoparticles by ATCC 393 alleviate the intestinal permeability, mitochondrial dysfunction and mitophagy induced by oxidative stress.

Food Funct 2021 Jun 22. Epub 2021 Jun 22.

The Key Laboratory for Space Bioscience and Biotechnology, School of Life Sciences, Northwestern Polytechnical University, Xi'an, Shaanxi 710072, China.

Selenium (Se) is an essential trace element. Nano-selenium has attracted great attention due to its various biological properties, especially strong antioxidant activity, high bioavailability, and low toxicity. Our previous studies demonstrated that the selenium nanoparticles (SeNPs) synthesized by Lactobacillus casei ATCC 393 (L. Read More

View Article and Full-Text PDF

Mitochondrial NAD(P)+ transhydrogenase: from molecular features to physiology and disease.

Antioxid Redox Signal 2021 Jun 22. Epub 2021 Jun 22.

State University of Campinas Faculty of Medical Sciences, 67791, Pathology, Rua Tessália Vieira de Camargo, 126., Campinas, Campinas, SP, Brazil, 13083-887;

Significance: Proton-translocating NAD(P)+ transhydrogenase, also known as nicotinamide nucleotide transhydrogenase (NNT), catalyzes a reversible reaction coupling the protonmotive force across the inner mitochondrial membrane and hydride (H-, a proton plus two electrons) transfer between the mitochondrial pools of NAD(H) and NADP(H). The forward NNT reaction is a source of NADPH in the mitochondrial matrix, fueling antioxidant and biosynthetic pathways with reductive potential. Despite the greater emphasis given to the net forward reaction, the reverse NNT reaction that oxidizes NADPH also occurs in physiological and pathological conditions. Read More

View Article and Full-Text PDF

The mitochondrial permeability transition pore: an evolving concept critical for cell life and death.

Biol Rev Camb Philos Soc 2021 Jun 21. Epub 2021 Jun 21.

CNC - Center for Neuroscience and Cell Biology, CIBB - Centre for Innovative Biomedicine and Biotechnology, University of Coimbra, UC Biotech, Biocant Park, Cantanhede, 3060-197, Portugal.

In this review, we summarize current knowledge of perhaps one of the most intriguing phenomena in cell biology: the mitochondrial permeability transition pore (mPTP). This phenomenon, which was initially observed as a sudden loss of inner mitochondrial membrane impermeability caused by excessive calcium, has been studied for almost 50 years, and still no definitive answer has been provided regarding its mechanisms. From its initial consideration as an in vitro artifact to the current notion that the mPTP is a phenomenon with physiological and pathological implications, a long road has been travelled. Read More

View Article and Full-Text PDF

Expression of LONP1 Is High in Visceral Adipose Tissue in Obesity, and Is Associated with Glucose and Lipid Metabolism.

Endocrinol Metab (Seoul) 2021 Jun 22. Epub 2021 Jun 22.

Department of Internal Medicine, Chungnam National University College of Medicine, Daejeon, Korea.

Background: The nature and role of the mitochondrial stress response in adipose tissue in relation to obesity are not yet known. To determine whether the mitochondrial unfolded protein response (UPRmt) in adipose tissue is associated with obesity in humans and rodents.

Methods: Visceral adipose tissue (VAT) was obtained from 48 normoglycemic women who underwent surgery. Read More

View Article and Full-Text PDF

Knockout of FosB gene changes drug sensitivity and invasion activity via the regulation of Bcl-2, E-cadherin, β-catenin, and vimentin expression.

Biochem Biophys Res Commun 2021 Jun 18;567:131-137. Epub 2021 Jun 18.

Department of Biological Sciences, College of Natural Sciences, Kangwon National University, Chuncheon, 24341, Republic of Korea. Electronic address:

Our previous research suggested the presence of a novel SETDB1-mediated FosB pathway that could be responsible for the regulation of cell proliferation and invasiveness during anticancer treatments. In this study, we prepared FosB knock-out (FosB-KO) A549 human lung cancer cells using the CRISPR/Cas9 system and examined the physiological and molecular changes that caused. Annexin V and TUNEL assays showed that FosB-KO clones were less sensitive to doxorubicin treatment compared to the control A549 cells. Read More

View Article and Full-Text PDF

Platinum nanoparticles inhibit intracellular ROS generation and protect against cold atmospheric plasma-induced cytotoxicity.

Nanomedicine 2021 Jun 18:102436. Epub 2021 Jun 18.

BioPlasma Research Group, School of Food Science and Environmental Health, Technological University Dublin, Dublin, Ireland; Nanolab, FOCAS Research Institute, Technological University Dublin, Dublin, Ireland. Electronic address:

Platinum nanoparticles (PtNPs) have been investigated for their antioxidant abilities in a range of biological and other applications. The ability to reduce off-target CAP cytotoxicity would be useful in Plasma Medicine, however, little has been published to date about the ability of PtNPs to reduce or inhibit the effects of CAP. Here we investigate whether PtNPs can protect against CAP-induced cytotoxicity in cancerous and non-cancerous cell lines. Read More

View Article and Full-Text PDF

Glucose fluctuation accelerates cardiac injury of diabetic mice via sodium-dependent glucose cotransporter 1 (SGLT1).

Arch Biochem Biophys 2021 Jun 18:108968. Epub 2021 Jun 18.

Department of General Medicine, The First Affiliated Hospital of Harbin Medical University, Harbin, 150001, People's Republic of China.

Recent studies have shown that blood glucose fluctuation is associated with complications of diabetes mellitus (DM). SGLT1 (sodium-dependent glucose cotransporter 1), is highly expressed in pathological conditions of heart, and is expressed cardiomyocytes induced by high glucose. Herein, we constructed a diabetic mouse model with glucose fluctuation to investigate whether SGLT1 is involved in glucose fluctuation-induced cardiac injury. Read More

View Article and Full-Text PDF

Introduction to the Theme on Membrane Channels.

Annu Rev Biochem 2021 Jun;90:503-505

Department of Biochemistry and Biophysics, Stockholm University, 106 91 Stockholm, Sweden; email:

This volume of the contains three reviews on membrane channel proteins: the first by Szczot et al., titled The Form and Function of PIEZO2; the second by Ruprecht & Kunji, titled Structural Mechanism of Transport of Mitochondrial Carriers; and the third by McIlwain et al., titled Membrane Exporters of Fluoride Ion. Read More

View Article and Full-Text PDF

and antifungal activity of buparvaquone against .

Antimicrob Agents Chemother 2021 Jun 21:AAC0069921. Epub 2021 Jun 21.

Laboratory of Fungal Cell Biology, Institute of Biophysics Carlos Chagas Filho, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.

Sporotrichosis has become an important zoonosis in Brazil and is the primary species transmitted by cats. Improvement of animal treatment will help control and limit the spread and geographic expansion of sporotrichosis. Accordingly, buparvaquone, an antiprotozoal hydroxynaphthoquinone agent marketed as Butalex®, was evaluated and against feline-borne isolates of . Read More

View Article and Full-Text PDF

Dissecting the Nucleoside Antibiotics as Universal Translation Inhibitors.

Acc Chem Res 2021 Jun 21. Epub 2021 Jun 21.

Department of Chemistry, University of Utah, Salt Lake City Utah 84103, United States.

ConspectusWithout question, natural products have provided the lion share of leads, if not drugs themselves, for the treatment of bacterial infections. The bacterial arms race, fueled by selection and survival pressures has delivered a natural arsenal of small molecules targeting the most essential of life processes. Antibiotics that target these critical intracellular processes face the formidable defense of both penetrating a bacterial cell membrane and avoiding efflux to exert their effect. Read More

View Article and Full-Text PDF

Genomic basis of deep-water adaptation in Arctic Charr (Salvelinus alpinus) morphs.

Mol Ecol 2021 Jun 21. Epub 2021 Jun 21.

Fisheries and Oceans Canada, Northwest Atlantic Fisheries Centre, St. John's, Newfoundland and Labrador, Canada.

The post-glacial colonization of Gander Lake in Newfoundland, Canada, by Arctic Charr (Salvelinus alpinus) provides the opportunity to study the genomic basis of adaptation to extreme deep-water environments. Colonization of deep-water (> 50 m) habitats often requires extensive adaptation to cope with novel environmental challenges from high hydrostatic pressure, low temperature, and low light, but the genomic mechanisms underlying evolution in these environments are rarely known. Here, we compare genomic divergence between a deep-water morph adapted to depths of up to 288m and a larger, piscivorous pelagic morph occupying shallower depths. Read More

View Article and Full-Text PDF

Honokiol Restores Microglial Phagocytosis by Reversing Metabolic Reprogramming.

J Alzheimers Dis 2021 Jun 17. Epub 2021 Jun 17.

Innovation Center for Neurological Disorders and Department of Neurology, Xuanwu Hospital, Capital Medical University, National Clinical Research Center for Geriatric Diseases, Beijing, China.

Background: Dysfunction of microglia has been increasingly recognized as a causative factor in Alzheimer's disease (AD); thus, developing medicines capable of restoring microglial functions is critically important and constitutes a promising therapeutic strategy. Honokiol is a natural neuroprotective compound extracted from Magnolia officinalis, which may play roles in AD therapy.

Objective: This study aimed to evaluate the role and the underlying mechanisms of honokiol in microglial phagocytosis. Read More

View Article and Full-Text PDF

Lactate Modulates Cellular Metabolism Through Histone Lactylation-Mediated Gene Expression in Non-Small Cell Lung Cancer.

Front Oncol 2021 2;11:647559. Epub 2021 Jun 2.

Department of Scientific Research Office, Affiliated Hospital of Qinghai University, Xining, China.

Lactate has been observed to fuel TCA cycle and is associated with cancer progression in human lung cancer, the leading cause of cancer deaths worldwide, but the effect of lactate on lung cancer metabolism is rarely reported. In this study, disordered metabolism in non-small cell lung cancer was demonstrated by increased G6PD and SDHA protein levels immunofluorescence, and up-regulated lactate dehydrogenase was found to be associated with poor prognosis. Then flow cytometry and Seahorse XFe analyzer were utilized to detect the effect of lactate on glycolysis and mitochondrial function in non-small cell lung cancer cells. Read More

View Article and Full-Text PDF

Optical Control of CD8 T Cell Metabolism and Effector Functions.

Front Immunol 2021 3;12:666231. Epub 2021 Jun 3.

Department of Microbiology and Immunology, David H. Smith Center for Vaccine Biology and Immunology, University of Rochester Medical Center, Rochester, NY, United States.

Although cancer immunotherapy is effective against hematological malignancies, it is less effective against solid tumors due in part to significant metabolic challenges present in the tumor microenvironment (TME), where infiltrated CD8 T cells face fierce competition with cancer cells for limited nutrients. Strong metabolic suppression in the TME is often associated with impaired T cell recruitment to the tumor site and hyporesponsive effector function T cell exhaustion. Increasing evidence suggests that mitochondria play a key role in CD8 T cell activation, effector function, and persistence in tumors. Read More

View Article and Full-Text PDF

PK11195 Protects From Cell Death Only When Applied During Reperfusion: Succinate-Mediated Mechanism of Action.

Front Physiol 2021 4;12:628508. Epub 2021 May 4.

Department of Molecular Biosciences, School of Veterinary Medicine, University of California, Davis, Davis, CA, United States.

: Reperfusion after myocardial ischemia causes cellular injury, in part due to changes in mitochondrial Ca handling, oxidative stress, and myocyte energetics. We have previously shown that the 18-kDa translocator protein of the outer mitochondrial membrane (TSPO) can modulate Ca handling. Here, we aim to evaluate the role of the TSPO in ischemia/reperfusion (I/R) injury. Read More

View Article and Full-Text PDF

Treatment of breast and colon cancer cell lines with anti-helmintic benzimidazoles mebendazole or albendazole results in selective apoptotic cell death.

J Cancer Res Clin Oncol 2021 Jun 20. Epub 2021 Jun 20.

Department of Pharmacology and Toxicology, School of Biomedical Sciences, University of Otago, Dunedin, New Zealand.

Purpose: Anti-helmintic drugs mebendazole and albendazole are commonly used to treat a variety of parasitic infections. They have recently shown some promising results in pre-clinical in vitro and in vivo anti-cancer studies.

Methods: We compare their efficacy in breast and colon cancer cell lines as well as in non-cancerous cells and elucidate their mechanism of action. Read More

View Article and Full-Text PDF

Mitochondrial Ca oscillation induces mitophagy initiation through the PINK1-Parkin pathway.

Cell Death Dis 2021 Jun 19;12(7):632. Epub 2021 Jun 19.

School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, China.

Dysregulation of the PINK1/Parkin-mediated mitophagy is essential to Parkinson's disease. Although important progress has been made in previous researches, the biochemical reagents that induce global and significant mitochondrial damage may still hinder deeper insights into the mechanisms of mitophagy. The origin of PINK1/Parkin pathway activation in mitophagy remains elusive. Read More

View Article and Full-Text PDF