233 results match your criteria mir-155 transfection

lncRNA NORAD promotes the progression of osteosarcoma via targeting of miR-155-5p.

Exp Ther Med 2021 Jun 18;21(6):645. Epub 2021 Apr 18.

Department of Orthopedics, Ezhou Central Hospital, Ezhou, Hubei 436000, P.R. China.

Osteosarcoma (OS) is the most common malignant bone tumor in teens. Non-coding RNA activated by DNA damage (NORAD), a long non-coding RNA (lncRNA), has been reported to be involved in cancer biology, although its role in OS remains largely unknown. In the present study reverse transcription-quantitative PCR (RT-qPCR) was used to determine the expression levels of NORAD and miR-155-5p in samples from patients with OS. Read More

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Research on the effect of interfering with miRNA-155 on triple-negative breast cancer cells.

Genes Genomics 2021 Apr 28. Epub 2021 Apr 28.

Department of Thyroid and Breast Diagnosis and Treatment Center, Shulan (Hangzhou) Hospital Affiliated to Zhejiang Shuren University Shulan International Medical College, Hangzhou, 310000, China.

Background: Triple negative breast cancer (TNBC) is a poor prognosis breast cancer with the highest mutation rate and limited treatment options. MiR-155 is highly expressed in TNBC, but its role and potential mechanism in TNBC remain to be elucidated.

Objective: The aim of this study is to examine the effect of interfering with miRNA-155 on the inflammatory pathway of NLRP 3 in TNBC (MDA-MB-231). Read More

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MiR-155 Inhibits Malignant Biological Behavior of Human Liver Cancer Cells by Regulating SRPK1.

Technol Cancer Res Treat 2021 Jan-Dec;20:1533033820957021

Department of Hepatobiliary Surgery, The Affiliated Hospital of Shananxi University of Chinese Medicine, Xianyang, China.

Although the treatment of liver cancer has made great progress, the mechanism of its occurrence is not completely clear. miR-155 plays an important regulatory role in tumorigenesis and development, including survival, proliferation, migration and invasion. However, the role and regulatory mechanism of miR-155 in liver cancer has rarely been reported. Read More

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The miR-155-5p inhibits osteoclast differentiation through targeting CXCR2 in orthodontic root resorption.

J Periodontal Res 2021 Mar 24. Epub 2021 Mar 24.

Department of Orthodontics, Dalian Stomatological Hospital, Dalian, China.

Background And Objective: Root resorption is an unavoidable side effect of orthodontic tooth movement. The mechanism of root resorption is similar to bone resorption; the odontoclasts share similar characteristics with osteoclasts (OCs). MicroRNAs (miRNAs) such as miR-155-5p play an important role in OC differentiation, but the underlying molecular mechanism of miR-155-5p in this process is not fully understood. Read More

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MicroRNA-155-5p promotes tumor progression and contributes to paclitaxel resistance via TP53INP1 in human breast cancer.

Pathol Res Pract 2021 Apr 5;220:153405. Epub 2021 Mar 5.

Departmentof Biochemistry and Molecular Biology, Bengbu Medical College, Anhui, 233030, China; Anhui Province Key Laboratory of Translational Cancer Research, Bengbu Medical College, Anhui, 233030, China. Electronic address:

MicroRNAs (miRNAs/miRs) are small, non-coding RNAs that are reported to serve numerous important regulatory functions; however, the role of miRNAs in regulating breast cancer cell biology remains poorly understood. Accumulating evidence has demonstrated that miRNAs orchestrate multiple cellular functions and serve crucial roles in cell differentiation and cancer development, either by acting as tumor suppressors or oncogenes. In particular, miR-155-5p expression levels have been found to be upregulated and serve as a prognostic marker in numerous types of solid cancer, including human breast cancer. Read More

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MiR-155 acts as an inhibitory factor in atherosclerosis-associated arterial pathogenesis by down-regulating NoxA1 related signaling pathway in ApoE mouse.

Cardiovasc Diagn Ther 2021 Feb;11(1):1-13

Department of Cardiology, Tongji Hospital affiliated to Tongji University, Shanghai, China.

Background: To investigate the protective efficacy of miR-155 on down regulating NADPH oxidase isoform subunit A1 (NoxA1) gene expression, resulting in inhibition of VSMC migration and over proliferation and thus ameliorating the progression of arterial atherosclerosis in AS mouse model. Therefore, to further explore the regulatory effect of miR-155 on neointima formation in AS and locate potential anti-atherosclerosis target.

Methods: The mouse vascular aorta smooth muscle cell (MOVAS) was cultured and transfected with recombinant Pad2YFG adenovirus fluorescent vector with miR-155 fragment into 4 groups. Read More

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February 2021

Inhibiting microRNA-155 attenuates atrial fibrillation by targeting CACNA1C.

J Mol Cell Cardiol 2021 Feb 24;155:58-65. Epub 2021 Feb 24.

Key Laboratory of Interdisciplinary Research, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China; Medical School of University of Chinese Academy of Sciences, Beijing, China; Guangzhou Regenerative Medicine and Health Guangdong Laboratory, Guangzhou, China. Electronic address:

Background: Reduction in L-type Ca current (I) density is a hallmark of the electrical remodeling in atrial fibrillation (AF). The expression of miR-155, whose predicted target gene is the α1c subunit of the calcium channel (CACNA1C), was upregulated in atrial cardiomyocytes (aCMs) from patients with paroxysmal AF.The study is to determine miR-155 could target the gene expression of I and contribute to electrical remodeling in AF. Read More

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February 2021

The antimicrobial peptide YD attenuates inflammation miR-155 targeting CASP12 during liver fibrosis.

Acta Pharm Sin B 2021 Jan 12;11(1):100-111. Epub 2020 Jul 12.

Key Laboratory of Preclinical Study for New Drugs of Gansu Province, School of Basic Medical Sciences & Research Unit of Peptide Science, Chinese Academy of Medical Sciences, 2019RU066, Lanzhou University, Lanzhou 730000, China.

The antimicrobial peptide APKGVQGPNG (named YD), a natural peptide originating from CBSYD1, exhibited excellent antibacterial and antioxidant properties . These characteristics are closely related to inflammatory responses which is the central trigger for liver fibrosis. However, the therapeutic effects of YD against hepatic fibrosis and the underlying mechanisms are rarely studied. Read More

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January 2021

miR-155 promotes proliferation and epithelial-mesenchymal transition of MCF-7 cells.

Exp Ther Med 2021 Mar 15;21(3):218. Epub 2021 Jan 15.

Department of Surgery, Xinganmeng People's Hospital, Ulanhot, Inner Mongolia 137400, P.R. China.

Breast cancer (BC) is the second leading cause of cancer-associated deaths among women worldwide. Increasing evidence has indicated that microRNAs (miRNAs) have demonstrated great potential for improving the diagnosis and therapy for BC. In the present study, miRNA-155 was detected in human BC tissues using reverse transcription-quantitative (RT-q)PCR. Read More

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Downregulation of miR-155-5p enhances the anti-tumor effect of cetuximab on triple-negative breast cancer cells via inducing cell apoptosis and pyroptosis.

Aging (Albany NY) 2021 01 5;13(1):228-240. Epub 2021 Jan 5.

State Key Laboratory of Bioreactor Engineering and Shanghai Key Laboratory of New Drug Design, School of Pharmacy, East China University of Science and Technology, Shanghai 200237, P.R. China.

Cetuximab resistance is the main obstacle for the treatment of EGFR overexpression cancer, including triple-negative breast cancer (TNBC). MicroRNA (miRNA)-155-5p is upregulated in TNBC cells; thus, the present study explored whether the downregulation of miR-155-5p enhanced the anti-tumor effect of cetuximab in TNBC cells. MDA-MB-231 and MDA-MB-468 cells were infected with lentivirus-epidermal growth factor receptor (EGFR) for 72 h to obtain EGFR-overexpressed cell lines (MDA-MB-231 and MDA-MB-468). Read More

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January 2021

Pivotal role of long non-coding ribonucleic acid-X-inactive specific transcript in regulating immune checkpoint programmed death ligand 1 through a shared pathway between miR-194-5p and miR-155-5p in hepatocellular carcinoma.

World J Hepatol 2020 12;12(12):1211-1227

Molecular Pharmacology Research Group, Department of Pharmacology and Toxicology, Faculty of Pharmacy and Biotechnology, German University in Cairo, Cairo 11835, Egypt.

Background: Anti-programmed death therapy has thrust immunotherapy into the spotlight. However, such therapy has a modest response in hepatocellular carcinoma (HCC). Epigenetic immunomodulation is a suggestive combinatorial therapy with immune checkpoint blockade. Read More

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December 2020

Inhibition of miR-155 alleviates sepsis-induced inflammation and intestinal barrier dysfunction by inactivating NF-κB signaling.

Int Immunopharmacol 2021 Jan 6;90:107218. Epub 2020 Dec 6.

Department of Intensive Care Unit, The First Affiliated Hospital of Wannan Medical College, Wuhu 241001, Anhui, China. Electronic address:

MicroRNA-155 (miR-155) is implicated in the pathological processes of sepsis. However, the function and regulatory mechanism of miR-155 in sepsis-induced inflammation and intestinal barrier dysfunction remain unknown. In this study, mouse models of sepsis were established by caecal ligation and puncture (CLP). Read More

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January 2021

MicroRNA-23a-3p Down-Regulation in Active Pulmonary Tuberculosis Patients with High Bacterial Burden Inhibits Mononuclear Cell Function and Phagocytosis through TLR4/TNF-α/TGF-β1/IL-10 Signaling via Targeting IRF1/SP1.

Int J Mol Sci 2020 Nov 14;21(22). Epub 2020 Nov 14.

Division of Pulmonary and Critical Care Medicine, Department of Medicine, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Kaohsiung 83301, Taiwan.

The aim of this study is to explore the role of microRNAs (miR)-21/23a/146a/150/155 targeting the toll-like receptor pathway in active tuberculosis (TB) disease and latent TB infection (LTBI). Gene expression levels of the five miRs and predicted target genes were assessed in peripheral blood mononuclear cells from 46 patients with active pulmonary TB, 15 subjects with LTBI, and 17 non-infected healthy subjects (NIHS). THP-1 cell lines were transfected with miR-23a-3p mimics under stimuli with Mycobacterium TB-specific antigens. Read More

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November 2020

Proliferation and Apoptosis of B-Cell Lymphoma Cells under Targeted Regulation of FOXO3 by miR-155.

Mediterr J Hematol Infect Dis 2020 1;12(1):e2020073. Epub 2020 Nov 1.

Department of Hematology and Rheumatology, The First Affiliated Hospital of Fujian Medical University, Fuzhou 350000, P.R. China.

This study aimed to explore B-cell lymphoma cells' proliferation and apoptosis under targeted regulation of FOXO3 by miR-155. We analyzed the differences between B-cell lymphoma cells and B lymphocytes in expressions of miR-155 and FOXO3, explored the effects of miR-155 on proliferation and apoptosis of B-cell lymphoma cells, and relevant mechanisms, and also analyzed the relationship between expressions of miR-155 and FOXO3 in 42 patients with diffuse large B-cell lymphoma (DLBCL) and clinical characteristics of them. B-cell lymphoma cells showed a higher expression of miR-155 and a low expression of FOXO3 than B lymphocytes (both P<0. Read More

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November 2020

Fusion protein engineered exosomes for targeted degradation of specific RNAs in lysosomes: a proof-of-concept study.

J Extracell Vesicles 2020 Sep 6;9(1):1816710. Epub 2020 Sep 6.

Department of Ultrasound Diagnostics, Tangdu Hospital, Fourth Military Medical University, Xi'an, People's Republic of China.

Therapeutically intervening the function of RNA in vivo remains a big challenge. We here developed an exosome-based strategy to deliver engineered RNA-binding protein for the purpose of recruiting specific RNA to the lysosomes for degradation. As a proof-of-principle study, RNA-binding protein HuR was fused to the C-terminus of Lamp2b, a membrane protein localized in both exosome and lysosome. Read More

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September 2020

MicroRNA-155-5p inhibits the invasion and migration of prostate cancer cells by targeting SPOCK1.

Oncol Lett 2020 Dec 11;20(6):353. Epub 2020 Oct 11.

Department of Urology, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215006, P.R. China.

The aim of the present study was to determine the effect of microRNA (miR)-155-5p on the expression of testican-1 (SPOCK1) and the invasion and migration of prostate cancer cells . Bioinformatics analysis and molecular biology assays revealed that SPOCK1 may be a direct target gene of miR-155-5p. In addition, a negative correlation was identified between SPCOK1 and miR-155-5p expression in prostate tumor tissues and cell lines. Read More

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December 2020

MicroRNA-155-5p Diminishes in Vitro Ovarian Cancer Cell Viability by Targeting HIF1α Expression.

Adv Pharm Bull 2020 Sep 9;10(4):630-637. Epub 2020 Aug 9.

Medicine and Health Sciences of Doctoral Program, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada, Farmako Yogyakarta 55281, Indonesia.

Ovarian cancer is the most lethal of gynecological malignancies. Recently, the development of microRNA (miRNA) -based therapeutics that could impact broad cellular programs, leading to inhibition of cancer cell viability, is gaining attention in the therapeutic landscape. The therapy is based on the presence of aberrant expressions of miRNA in cancer cells. Read More

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September 2020

Next generation miRNA inhibition using short anti-seed PNAs encapsulated in PLGA nanoparticles.

J Control Release 2020 11 21;327:406-419. Epub 2020 Aug 21.

Department of Pharmaceutical Sciences, University of Connecticut, Storrs, CT 06269, USA. Electronic address:

Selective inhibition of microRNAs (miRNAs) offers a new avenue for cancer therapeutics. While most of the current anti-miRNA (antimiR) reagents target full length miRNAs, here we investigate novel nanoparticle-delivered short PNA probes containing cationic domains targeting the seed region of the miRNA for effective antimiR therapy. For proof of concept, we tested PNAs targeting miRNA-155 and employed poly(lactic-co-glycolic acid) (PLGA)-based nanoparticle formulation for delivery. Read More

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November 2020

Apigenin attenuates TGF-β1-stimulated cardiac fibroblast differentiation and extracellular matrix production by targeting miR-155-5p/c-Ski/Smad pathway.

J Ethnopharmacol 2021 Jan 12;265:113195. Epub 2020 Aug 12.

Department of Pharmacology, College of Pharmaceutical Sciences, Soochow University, Suzhou, 215123, Jiangsu Province, China. Electronic address:

Ethnopharmacological Relevance: Apigenin is a natural flavonoid compound present in chamomile (Matricaia chamomilla L.) from the Asteraceae family, which is used in the treatment of cardiovascular diseases by traditional healers, but its effects on differentiation and extracellular matrix (ECM) production of cardiac fibroblasts (CFs) induced by transforming growth factor beta 1 (TGF-β1) are poorly understood.

Aim Of The Study: This study aimed to examine these effects and potential molecular mechanisms and to provide a new application of apigenin in the prevention and treatment of cardiac fibrosis. Read More

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January 2021

Downregulation of microRNA-155-5p prevents immune thrombocytopenia by promoting macrophage M2 polarization via the SOCS1-dependent PD1/PDL1 pathway.

Life Sci 2020 Sep 4;257:118057. Epub 2020 Jul 4.

Department of Hematology, the First Affiliated Hospital of Harbin Medical University, Harbin 150007, PR China. Electronic address:

Aims: We set about to investigate the potential role of microRNA-155-5p (miR-155-5p) in the development of immune thrombocytopenia (ITP), an idiopathic deficiency of blood platelets.

Main Methods: Initially, RT-qPCR and Western blot analyses were carried out to determine the expression of miR-155-5p and SOCS1 in peripheral blood mononuclear cells (PBMCs) and macrophages from ITP patients. We undertook gain- and loss- function methods by transfection of macrophages and PBMCs with treated plasmids. Read More

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September 2020

Role of MicroRNAs in regulation of DNA damage in monocytes exposed to polystyrene and TiO nanoparticles.

Toxicol Rep 2020 3;7:743-751. Epub 2020 Jun 3.

Chair for Fish Diseases and Fisheries Biology, Faculty of Veterinary Medicine, Ludwig Maximilian University of Munich, Munich, Germany.

The release of nanoparticles into the environment can interfere with the health of the exposed organisms. MicroRNAs have been suggested as potential toxicology biomarkers. The expression of potential zebrafish nano-toxicity biomarker miRNAs in our previous study was validated in THP-1 human monocytic cell line after exposure to polystyrene (PSNPs) and ARS labeled Titanium dioxide nanoparticles (nano-TiO-ARS). Read More

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Osteoclast signaling-targeting miR-146a-3p and miR-155-5p are downregulated in Paget's disease of bone.

Biochim Biophys Acta Mol Basis Dis 2020 10 30;1866(10):165852. Epub 2020 May 30.

Division of Rheumatology, Department of Medicine, Faculty of Medicine and Health Sciences, University of Sherbrooke, 3001, 12(th) Avenue North, Sherbrooke, PQ, Canada. Electronic address:

MicroRNA (miRs) are small, non-coding RNA that post-transcriptionally regulate DNA expression. We hypothesized that specific miR profiles may be a feature of overactive osteoclasts in Paget's disease of bone (PDB), a disorder characterized by an increased and disorganized bone remodeling that typically begins with excessive bone resorption. We compared the expression profile of 13 miRs in human osteoclasts differentiated in vitro from peripheral blood mononuclear cells (PBMCs) of patients with PDB (n = 10) or age- and sex- matched healthy subjects (n = 10). Read More

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October 2020

DsRNA induction of microRNA-155 disrupt tight junction barrier by modulating claudins.

Asia Pac Allergy 2020 Apr 27;10(2):e20. Epub 2020 Apr 27.

Division of Respiratory Medicine, Department of Internal Medicine, Nihon University School of Medicine, Tokyo, Japan.

Background: The impaired barrier function of the airway epithelium due to RNA virus infection is closely related to the development and exacerbation of allergic airway inflammation.

Objective: In this study, we investigated the roles of microRNAs on the mechanisms of double-stranded RNA (dsRNA)-induced epithelial barrier dysfunction.

Methods: 16HBE14o- human bronchial epithelial cells were grown to confluence on Transwell inserts and exposed to poly-I:C. Read More

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miR-155 Regulates the Proliferation of Glioma Cells Through PI3K/AKT Signaling.

Front Neurol 2020 28;11:297. Epub 2020 Apr 28.

Department of Neurosurgery, Shandong Provincial ENT Hospital, Shandong Provincial ENT Hospital Affiliated to Shandong University, Jinan, China.

Micro-RNA plays a critical role in the pathological process of gliomas. Previous research showed that the level of miR-155 was significantly increased in many cancers, including gliomas. However, the mechanism of glioma is still unknown. Read More

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Suppression of microRNA-155 exerts an anti-inflammatory effect on CD4+ T cell-mediated inflammatory response in the pathogenesis of atherosclerosis.

Acta Biochim Biophys Sin (Shanghai) 2020 Jun;52(6):654-664

Department of Cardiac Surgery, Zhongshan Hospital, Fudan University, Shanghai 200032, China.

In the current study, we aimed to investigate the effects of miR-155 on CD4+ T cell-mediated immune response in the pathogenesis of atherosclerosis. CD34+ hematopoietic stem cells, CD4+ T lymphocytes, endothelial cells (ECs), and vascular smooth muscle cells (VSMCs) were harvested from the same donor. Knockdown of miR-155 in the CD4+ T cells was achieved by lentiviral transfection, whereas control RNA-transfected or untransfected lymphocytes were used as controls. Read More

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Profiling of miRNAs in Mouse Peritoneal Macrophages Responding to Infection.

Front Cell Infect Microbiol 2020 3;10:132. Epub 2020 Apr 3.

State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Gansu, China.

Alveolar echinococcosis (AE) is a zoonotic helminthic disease caused by infection with the larval of in human and animals. Here, we compared miRNA profiles of the peritoneal macrophages of -infected and un-infected female BALB/c mice using high-throughput sequencing. A total of 87 known miRNAs were differentially expressed (fold change ≥ 2, < 0. Read More

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Highly efficient serum-free manipulation of miRNA in human NK cells without loss of viability or phenotypic alterations is accomplished with TransIT-TKO.

PLoS One 2020 17;15(4):e0231664. Epub 2020 Apr 17.

Department of Microbiology and Immunology, Dalhousie University, Halifax, Canada.

Natural killer (NK) cells are innate lymphocytes with functions that include target cell killing, inflammation and regulation. NK cells integrate incoming activating and inhibitory signals through an array of germline-encoded receptors to gauge the health of neighbouring cells. The reactive potential of NK cells is influenced by microRNA (miRNA), small non-coding sequences that interfere with mRNA expression. Read More

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MiR-155 inhibition alleviates suppression of osteoblastic differentiation by high glucose and free fatty acids in human bone marrow stromal cells by upregulating SIRT1.

Pflugers Arch 2020 04 4;472(4):473-480. Epub 2020 Apr 4.

Orthopaedics, The First Affiliated Hospital of Chengdu Medical College, No. 278 Baoguang Avenue, Xindu District, Chengdu, 610500, China.

Diabetic osteoporosis is a severe and chronic complication of diabetes in the bone and joint system, and its pathogenesis is needed to be explored. In the present study, we examined the effect and underlying mechanism of miR-155 on osteogenic differentiation in human bone marrow-derived mesenchymal stem cells (hBMSCs) under high glucose and free fatty acids (HG-FFA) conditions. It was shown that miR-155 levels in hBMSCs increased corresponding to the time of exposure to HG-FFA treatment. Read More

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Abnormal lncRNA CCAT1/microRNA-155/SIRT1 axis promoted inflammatory response and apoptosis of tubular epithelial cells in LPS caused acute kidney injury.

Mitochondrion 2020 07 31;53:76-90. Epub 2020 Mar 31.

Department of Nephrology, First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan Province, The People's Republic of China. Electronic address:

Backgrounds: Acute kidney injury (AKI) is characterized by excessive inflammatory response and apoptosis in tubular epithelial cells. Recent studies suggested that long non-coding RNAs colon cancer-associated transcript-1 (CCAT-1) and microRNA-155 (miR-155) might regulate cell death and inflammation. We aimed to explore the role of CCAT-1/miRNA-155 axis in the AKI. Read More

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GuaLou GuiZhi decoction represses LPS-induced BV2 activation via miR-155 induced inflammatory signals.

Pak J Pharm Sci 2020 Jan;33(1(Special)):403-408

Institute of Integrated Chinese and Western Medicine, Fujian University of Traditional Chinese Medicine, 1 Qiuyang Road, Minhou Shangjie, Fuzhou, Fujian, China.

Inflammatory response that occur post-ischemia is a serious problem in the treatment of ischemic brain disease. MicroRNA-155 is a brain-specific or brain-enriched miRNA, which mediates inflammatory reactions in cerebral ischemic tissue by regulating inflammatory signal and the expression level of SOCS1. The present study was aimed to assess the effect of GuaLou GuiZhi Decoction (GLGZD) on miR-155 expression in activated microglia following inflammation and further explore the role of GLGZD on expression of the inflammation-related gene. Read More

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January 2020