Diabetologia 2016 12 27;59(12):2654-2663. Epub 2016 Sep 27.
Montreal Diabetes Research Center, CRCHUM, 900 St-Denis (Viger Tower), Room R08-412, Montreal, QC, H1W 4A4, Canada.
Aims/hypothesis: To directly assess the role of beta cell lipolysis in insulin secretion and whole-body energy homeostasis, inducible beta cell-specific adipose triglyceride lipase (ATGL)-deficient (B-Atgl-KO) mice were studied under normal diet (ND) and high-fat diet (HFD) conditions.
Methods: Atgl mice were cross-bred with Mip-Cre-ERT mice to generate Mip-Cre-ERT;Atgl mice. At 8 weeks of age, these mice were injected with tamoxifen to induce deletion of beta cell-specific Atgl (also known as Pnpla2), and the mice were fed an ND or HFD. Read More