363 results match your criteria minutes mtt

Mechanical and antimicrobial effects of riboflavin-mediated photosensitization of in vitro C. albicans formed on polymethyl methacrylate resin.

Photodiagnosis Photodyn Ther 2021 Aug 17:102488. Epub 2021 Aug 17.

Department of Prosthetic Dental Sciences, College of Dentistry, Jazan University, Jazan, Saudi Arabia. Electronic address:

Purpose: This study assessed the potential of riboflavin-mediated photodynamic therapy (RF-PDT) against Candida albicans (C. albicans) and compare the effects of RF-PDT with other therapeutic modalities in terms of mechanical and surface characteristics of acrylic denture base material.

Materials And Methods: Molds (10 × 10 × 2 mm) of acrylic resin specimens were prepared and underwent artificial ageing. Read More

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MTT and Blood-Brain Barrier Disruption within Asymptomatic Vascular WM Lesions.

AJNR Am J Neuroradiol 2021 08 3;42(8):1396-1402. Epub 2021 Jun 3.

Department of Neurology (R.L., P.N.), Electrical and Computer Engineering (B.E.D., J.L.P.), Johns Hopkins University, Baltimore, Maryland

Background And Purpose: White matter lesions of presumed ischemic origin are associated with progressive cognitive impairment and impaired BBB function. Studying the longitudinal effects of white matter lesion biomarkers that measure changes in perfusion and BBB patency within white matter lesions is required for long-term studies of lesion progression. We studied perfusion and BBB disruption within white matter lesions in asymptomatic subjects. Read More

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Hypnotic Effect of . Hydroalcoholic Extract in Pentobarbital-Treated Mice.

Neurol Res Int 2021 21;2021:5521019. Epub 2021 Apr 21.

Neuroscience Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.

Background: Current drugs used in the management of insomnia are associated with side effects. The use of medicinal herbs for insomnia treatment has recently been suggested.

Objective: The present study aimed to determine the hypnotic activity of the hydroalcoholic extract of . Read More

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Comparative study on the local tolerance and efficacy of benzalkonium chloride, myristalkonium chloride and nonoxynol-9 as active principles in vaginal contraceptives.

Eur J Contracept Reprod Health Care 2021 Aug 6;26(4):334-342. Epub 2021 Apr 6.

Biology of Reproduction and Stem Cells Group, CNC- Center for Neuroscience and Cell Biology, CIBB, Polo III, Azinhaga Santa Comba, University of Coimbra, Coimbra, Portugal.

Background: Spermicides have been identified as a potentially attractive alternative to hormonal contraceptives and/or intrauterine devices. Thus, this study aimed evaluating the efficacy and local tolerance of benzalkonium chloride (BKC) and myristalkonium chloride (MKC) contained in Pharmatex vaginal formulations and compare them with nonoxynol-9 (N-9), the most common active ingredient in topical vaginal contraceptives.

Methods: Human normozoospermic samples were assessed for motility, viability, acrosome status and penetration ability after exposure to control, N-9 or different BKC and MKC doses for 0 and 10 minutes. Read More

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[The adjuvant killing effect of modulated electro-hyperthermia combined with chemotherapy on B16F10 melanoma cells].

Magy Onkol 2021 Mar 21;65(1):71-77. Epub 2020 Feb 21.

Transzlációs Medicina Intézet, Semmelweis Egyetem, Budapest, Hungary.

Our aim was to detect the effect of modulated electro-hyperthermia (mEHT) on cell viability and to examine if hyperthermia can augment the cell killing effect of various chemotherapeutic agents. B16F10 melanoma cells were treated for 30, 60, 90 and 120 minutes with mEHT using LabEHY100 (OncothermTM). Cell viability was measured using MTT assay and apoptosis by annexin V/7-AAD staining using flow cytometry 24 hours post-treatment. Read More

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Cellular Effects of Ultraviolet-Radiated Reduced-Titanium Dioxide Nanoparticles on Human Hypopharyngeal Adenocarcinoma Cells.

J Nanosci Nanotechnol 2021 07;21(7):3656-3661

Department of Pharmacology and Dental Therapeutics, College of Dentistry, Chosun University, Gwangju, 501-759, South Korea.

The cellular effects of ultraviolet (UV)-radiated reduced-titanium dioxide (TiO₂) nanoparticles were investigated on human hypopharyngeal adenocarcinoma cells (FaDu). In 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, the viability of FaDu cells exposed to UV (254 nm) for 10 minutes, in the presence of reduced-TiO₂ nanoparticles in rutile, was dose- and time-dependently decreased. The UV-radiated reduced-TiO₂ suppressed the cell proliferation by inhibiting the expression of cell cycle kinase, cyclin dependent kinase 2 (Cdk2), and its functional regulators Cyclin E and Cyclin B1 as well as proliferation-regulating proteins of p85 regulatory sub-unit of phosphoinositide3-kinases (PI3K p85), phosphorylated protein kinase B (p-AKT/p-PKB) and phosphorylated mammalian target of rapamycin (p-mTOR). Read More

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Final Endodontic Irrigation with 2% Peracetic Acid: Antimicrobial Activity and Cytotoxicity.

Eur J Dent 2021 Jul 3;15(3):533-538. Epub 2021 Feb 3.

Postgraduate Program in Dentistry, Health Institute of Nova Friburgo, Fluminense Federal University, Nova Friburgo, Rio de Janeiro, Brazil.

Objective:  The aim of present study was to assess the cytotoxicity and antimicrobial efficacy of 2% peracetic acid (PAA) compared with 5.25% sodium hypochlorite (NaOCl) and 2% chlorhexidine (CHX).

Material And Methods:  For the cytotoxicity test, 100 µl of the tested solutions were added in 12 wells with ECV 304 endothelial cells in each group: NaOCl, CHX, and PAA, in addition to the control group. Read More

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Stoichiometrically Governed Curcumin Solid Dispersion and Its Cytotoxic Evaluation on Colorectal Adenocarcinoma Cells.

Drug Des Devel Ther 2020 2;14:4639-4658. Epub 2020 Nov 2.

Department of Pharmaceutical Analysis, Erode College of Pharmacy, Veppampalayam, Erode, Tamil Nadu 638112, India.

Background: Colorectal cancer (CRC) is the third most commonly occurring cancer in men and the second most commonly occurring cancer in women. Curcumin (CMN) is obtained from a natural source and has no toxicity, even at high doses (8,000 mg/kg body weight in 24 hours) and was determined to have anticancer potency on several kinds of carcinoma. However, its medical applications were limited because of its low solubility and poor bioavailability. Read More

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Cytotoxicity of the Ingredients of Commonly Used Toothpastes and Mouthwashes on Human Gingival Fibroblasts.

Front Dent 2019 Nov-Dec;16(6):450-457. Epub 2019 Dec 20.

Dental Research Center, Dentistry Research Institute, Tehran University of Medical Sciences, Tehran, Iran.

Objectives: Toothpastes and mouthwashes contain ingredients that may be toxic for oral mucosal tissues. This study aimed to assess the cytotoxicity of the ingredients of commonly used toothpastes and mouthwashes.

Materials And Methods: This experimental study was performed on 16 toothpastes and four mouthwashes widely available in the Iranian market. Read More

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December 2019

Effects of Lidocaine on Mitomycin C Cytotoxicity.

Ophthalmol Glaucoma 2021 May-Jun;4(3):330-335. Epub 2020 Oct 17.

Little Rock Eye Clinic, Little Rock, Arkansas.

Purpose: Intra-Tenon or subconjunctival injection of a solution of mitomycin C (MMC) and 1% preservative-free lidocaine (as an anesthetic) has gained popularity for its use in trabeculectomy, a filtering surgery for glaucoma. To our knowledge, no studies have analyzed the impact of lidocaine on the cytotoxic effects of MMC in this setting. This study was conducted to evaluate in vitro fibroblast cytotoxicity to a solution of MMC (0. Read More

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October 2020

Saikosaponin D Inhibits Proliferation and Promotes Apoptosis Through Activation of MKK4-JNK Signaling Pathway in Pancreatic Cancer Cells.

Onco Targets Ther 2020 24;13:9465-9479. Epub 2020 Sep 24.

College of Pharmaceutical Science, Zhejiang Chinese Medical University, Hangzhou 310053, People's Republic of China.

Introduction: Pancreatic cancer remains one of the most lethal malignancies and has few treatment options. Saikosaponin D (SSD), a major bioactive triterpene saponin isolated from , has been reported to exert cytotoxicity properties toward many cancer cells. However, the effects of SSD on pancreatic cancer have been little scrutinized. Read More

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September 2020

Effects of Na-DNA mouthwash solutions on oral soft tissues. A bioreactor-based reconstituted human oral epithelium model.

Am J Dent 2020 Oct;33(5):277-284

Department of Biomedical, Surgical and Dental Sciences, University of Milan, Milan, Italy,

Purpose: To investigate whether the addition of sodium-DNA (Na-DNA) to chlorhexidine (CHX)-containing mouthwash influenced morphology and viability of a reconstituted human oral epithelium (ROE), and protects ROE against oxidative stress.

Methods: Multi-layered 0.5 cm² ROE specimens were positioned inside a continuous flow bioreactor and grown air-lifted for 24 hours. Read More

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October 2020

Clinical Utility of the Meal Tolerance Test in the Care of Patients with Type 2 Diabetes Mellitus.

Intern Med 2020 15;59(18):2229-2235. Epub 2020 Sep 15.

Department of Internal Medicine, Wakayama Rosai Hospital, Japan.

Objective The measurement of C-peptide immunoreactivity (CPR) is essential for evaluating the pancreatic β-cell function and selecting appropriate therapeutic agents in patients with diabetes mellitus. The meal tolerance test (MTT) is simple to administer physiological insulin-stimulating test. Previous studies have reported that several CPR-related indices are useful markers for predicting insulin requirement in type 2 diabetes. Read More

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October 2020

Preparation and Evaluation of Doxorubicin-Loaded PLA-PEG-FA Copolymer Containing Superparamagnetic Iron Oxide Nanoparticles (SPIONs) for Cancer Treatment: Combination Therapy with Hyperthermia and Chemotherapy.

Int J Nanomedicine 2020 18;15:6167-6182. Epub 2020 Aug 18.

Nanobiotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.

Background: Among the novel cancer treatment strategies, combination therapy is a cornerstone of cancer therapy.

Materials And Methods: Here, combination therapy with targeted polymer, magnetic hyperthermia and chemotherapy was presented as an effective therapeutic technique. The DOX-loaded PLA-PEG-FA magnetic nanoparticles (nanocarrier) were prepared via a double emulsion method. Read More

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October 2020

Perfusion Parameter Thresholds That Discriminate Ischemic Core Vary with Time from Onset in Acute Ischemic Stroke.

AJNR Am J Neuroradiol 2020 10 27;41(10):1809-1815. Epub 2020 Aug 27.

From the Department of Neurology (T.Y., Y.Y., H.J., T.H., H.T., F.S., J.L.S., D.S.L.), David Geffen School of Medicine at University of California, Los Angeles, Los Angeles, California.

Background And Purpose: When mapping the ischemic core and penumbra in patients with acute ischemic stroke using perfusion imaging, the core is currently delineated by applying the same threshold value for relative CBF at all time points from onset to imaging. We investigated whether the degree of perfusion abnormality and optimal perfusion parameter thresholds for defining ischemic core vary with time from onset to imaging.

Materials And Methods: In a prospectively maintained registry, consecutive patients were analyzed who had ICA or M1 occlusion, baseline perfusion and diffusion MR imaging, treatment with IV tPA and/or endovascular thrombectomy, and a witnessed, well-documented time of onset. Read More

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October 2020

Noscapine protects the H9c2 cardiomyocytes of rats against oxygen-glucose deprivation/reperfusion injury.

Mol Biol Rep 2020 Aug 9;47(8):5711-5719. Epub 2020 Jul 9.

Department of Pharmacology and Toxicology, Faculty of Pharmacy and Pharmaceutical Sciences, Tehran Medical Science, Islamic Azad University, Tehran, Iran.

Noscapine is an antitumor alkaloid derived from Papaver somniferum plants. Our previous study has demonstrated that exposure of noscapine on primary murine fetal cortical neurons exposed to oxygen-glucose deprivation/reperfusion (OGD/R) has neuroprotective effects. In current study, the effects of noscapine on cardiomyocytes (H9c2 cells) damage caused by 120 minutes (min) of OGD/R were evaluated and we determined whether the addition of BD1047, sigma-one receptor antagonist, prevents the protective effects of noscapine in H9c2 cells through the production of nitric oxide (NO) and apoptosis. Read More

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The effect of dental gel formulation on human primary fibroblasts - an in vitro study.

Folia Histochem Cytobiol 2020 22;58(2):156-161. Epub 2020 Jun 22.

Department of Dental Surgery, Wroclaw Medical University, Wroclaw, Poland.

Introduction: In ordinary dental practice, the dentist often meets with patients suffering from ulcers, aphtha with edema, bleeding gums, bothersome burning, and dry mouth. These are prosthetic, orthodontic patients, after surgery, in various age ranges. Protefix® gel is a soothing and regenerating preparation aimed at patients with mucosal problems. Read More

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Neurogenic differentiation of human dental pulp stem cells by optogenetics stimulation.

J Chem Neuroanat 2020 11 5;109:101821. Epub 2020 Jun 5.

Laser and Plasma Research Institute-Shahid Beheshti University, Tehran, Iran.

Introduction: Human dental pulp stem cells (hDPSCs), a promising source for autologous transplantation in regenerative medicine, have been shown to be able to differentiate into neural precursors. Optogenetics is considered as an advanced biological technique in neuroscience which is able to control the activity of genetically modified stem cells by light. The purpose of this study is to investigate the neurogenic differentiation of hDPSCs following optogenetic stimulation. Read More

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November 2020

2,4-Thiazolidinedione as Precursor to the Synthesis of Compounds with Anti-glioma Activities in C6 and GL261 Cells.

Med Chem 2021 ;17(6):601-610

Laboratorio de Quimica Aplicada a Bioativos (LaQuiABio), Centro de Ciencias Quimicas, Farmaceuticas e de Alimentos, Universidade Federal de Pelotas, Campus Universitario s/n, Capao do Leao, RS, CEP: 96010-900, Brazil.

Background: Thiazolidinediones (TZDs) represent an important class of heterocyclic compounds that have versatile biological activities, including anticancer activity. Glioma is one of the most common primary brain tumors, and it is responsible for most of the deaths caused by primary brain tumors. In the present work, 2,4-thiazolidinediones were synthesized via a multicomponent microwave one-pot procedure. Read More

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Clinical Impact of Cryopreservation on Split Thickness Skin Grafts in the Porcine Model.

J Burn Care Res 2020 02;41(2):306-316

Center for Transplantation Science (CTS), Massachusetts General Hospital, Boston, Massachusetts, USA.

Vital, genetically engineered, porcine xenografts represent a promising alternative to human cadaveric allografts (HCA) in the treatment of severe burns. However, their clinical value would be significantly enhanced if preservation and long-term storage-without the loss of cellular viability-were feasible. The objective of this study was to examine the direct impact of cryopreservation and the length of storage on critical in vivo and in vitro parameters, necessary for a successful, potentially equivalent substitute to HCA. Read More

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February 2020

Dynamic Covalent C═C Bond, Cross-Linked, Injectable, and Self-Healable Hydrogels via Knoevenagel Condensation.

Biomacromolecules 2020 03 25;21(3):1234-1242. Epub 2020 Feb 25.

School of Materials Science and Engineering, Qingdao University, Qingdao 266071, China.

Hydrogels have a wide range of applications in the fields of biomedicine, flexible electronics, and bionics. In this study, injectable and self-healable hydrogels were first prepared based on a dynamic covalent C═C bond formed via the Knoevenagel condensation reaction between poly(ethylene glycol) dicyanoacetate and water-soluble poly(vanillin acrylate). Three kinds of catalysts (phosphate buffer, zeolitic imidazolate framework-8, and tertiary amine) were used in Knoevenagel condensation for preparing hydrogels. Read More

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MR-guided focused ultrasound cerebellothalamic tractotomy for chronic therapy-resistant essential tremor: anatomical target reappraisal and clinical results.

J Neurosurg 2020 Feb 7:1-10. Epub 2020 Feb 7.

Objective: In addition to the well-recognized ventral intermediate nucleus (Vim) thalamotomy for the treatment of chronic therapy-resistant essential tremor (ET), an alternative approach targeting the posterior part of the subthalamus was proposed in the 1960s and early 1970s and then was reactualized as cerebellothalamic tractotomy (CTT) with the advent of MR-guided focused ultrasound (MRgFUS) surgery. The goal of this study was to improve target coverage and thus efficacy (i.e. Read More

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February 2020

Combined Magnetic Resonance Imaging and Photodynamic Therapy Using Polyfunctionalised Nanoparticles Bearing Robust Gadolinium Surface Units.

Chemistry 2020 Apr 18;26(20):4552-4566. Epub 2020 Mar 18.

Department of Chemistry, Molecular Sciences Research Hub, Imperial College London, White City Campus, London, W12 0BZ, UK.

A robust dithiocarbamate tether allows novel gadolinium units based on DOTAGA (q=1) to be attached to the surface of gold nanoparticles (2.6-4.1 nm diameter) along with functional units offering biocompatibility, targeting and photodynamic therapy. Read More

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[Histone deacetylase 3 inhibitor alleviates alcohol-induced disruption of intestinal epithelial barrier via inhibiting nuclear factor κB].

Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi 2019 Sep;35(9):800-805

Department of Gastroenterology, First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710061, China. *Corresponding authors, E-mail:

Objective To investigate the role and mechanism of histone deacetylase 3 (HDAC3) in alcohol-induced inflammation and permeability of intestinal epithelial cells. Methods To select the proper concentration of alcohol, differentiated Caco-2 cells were treated with different concentrations (10, 25, 50, 100 and 200 mmol/L) of alcohol, and then cell viability was assayed by MTT assay; the mRNA and protein levels of HDAC3 were analyzed by real-time PCR and Western blot analysis. Differentiated Caco-2 cells were divided into three groups: control group, alcohol group (treatment with 50 mmol/L alcohol for 60 minutes), and alcohol combined with HDAC3 inhibitor group (pretreatment with 2 μmol/L RGFP966 1 hour before alcohol). Read More

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September 2019

Complex Effects of Putative DRP-1 Inhibitors on Stress Responses in Mouse Heart and Rat Cardiomyoblasts.

J Pharmacol Exp Ther 2020 01 8;372(1):95-106. Epub 2019 Nov 8.

School of Medical Science, Griffith University, Southport, Australia (L.W., J.V., E.D.T., J.N.P., J.P.H.) and Critical Care Research Group, The Prince Charles Hospital and The University of Queensland, Chermside, Australia (L.E.S.H.)

Dynamin-related protein-1 (DRP-1)-dependent mitochondrial fission may influence cardiac tolerance to ischemic or oxidative stress, presenting a potential "cardioprotective" target. Effects of dynamin inhibitors [mitochondrial division inhibitor 1 (MDIVI-1) and dynasore] on injury, mitochondrial function, and signaling proteins were assessed in distinct models: ischemia-reperfusion (I-R) in mouse hearts and oxidative stress in rat H9c2 cardiomyoblasts. Hearts exhibited substantial cell death [approx. Read More

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January 2020

Shotgun Lipidomics Discovered Diurnal Regulation of Lipid Metabolism Linked to Insulin Sensitivity in Nondiabetic Men.

J Clin Endocrinol Metab 2020 05;105(5)

Department of Clinical Nutrition, German Institute of Human Nutrition Potsdam-Rehbruecke, Nuthetal, Germany.

Context: Meal timing affects metabolic homeostasis and body weight, but how composition and timing of meals affect plasma lipidomics in humans is not well studied.

Objective: We used high throughput shotgun plasma lipidomics to investigate effects of timing of carbohydrate and fat intake on lipid metabolism and its relation to glycemic control.

Design: 29 nondiabetic men consumed (1) a high-carb test meal (MTT-HC) at 09. Read More

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Nanosilver Colloid Inhibits Tachyzoites and Bradyzoites in Vitro.

Iran J Parasitol 2019 Jul-Sep;14(3):362-367

Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.

Background: , the coccidian protozoan parasite with worldwide distribution, is the agent of toxoplasmosis. The disease is life threatening in congenital form and in immunocompromised patients. The present study was carried out in 2016 to evaluate the in vitro effects of nanosilver colloid on tachyzoites and bradyzoites of , RH and Tehran strains. Read More

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November 2019

Anti-inflammatory and anti-resorptive efficacy of adrenergic blockers on late replanted rat incisors.

Dent Traumatol 2020 Jun 13;36(3):253-263. Epub 2019 Nov 13.

Department of Restorative Dentistry, Endodontic Division, Institute of Science and Technology, São Paulo State University (Unesp), São José dos Campos, Brazil.

Background/aim: In addition to their anti-inflammatory and anti-osteoclastogenesis properties, adrenergic blockers may also have promising anti-resorptive effects that can prevent root resorption when teeth are replanted because of avulsion. The aim of this study was to investigate the effects of phentolamine (Ph) and propranolol (Pr) in gels on the repair process of late replanted rat incisors. A further aim was to evaluate the biocompatibility of both drugs to human periodontal ligament fibroblasts (HPDLFs). Read More

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Synergistic effect of photodynamic therapy at 400 nm and doxycycline against .

Future Microbiol 2019 09;14:1199-1205

Department of Experimental & Clinical Medicine, University of Florence, Florence, Italy.

The objective of this study was to investigate the possible synergy between doxycycline and photodynamic therapy against and to evaluate the possible side effects on adenocarcinoma gastric cells with and without protoporphyrin IX. Three strains (ATCC 700392, 43504 and 49503) were grown on solid medium either with, or without, doxycycline at subinhibitory concentrations, and irradiated for 10, 20 and 30 minutes with a 400 nm-peaked light source. The phototoxicity tests on AGS cells were evaluated by MTT assay. Read More

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September 2019

Biocompatibility and zinc release testing of a zinc-containing vaginal gel.

Menopause 2020 02;27(2):143-149

Eastern Virginia Medical School Department of Obstetrics and Gynecology, Division of Female Pelvic Medicine and Reconstructive Surgery, Norfolk, VA.

Objective: To test the biocompatibility of a zinc-containing vaginal gel, evaluate its ability to release zinc, and to assess the transepithelial permeability of zinc on human vaginal epithelium.

Methods: The release and membrane diffusion of zinc from the vaginal gel was tested by a vertical Franz-diffusion cell system. The biocompatibility of the gel was tested on HaCaT cells and reconstructed human vaginal epithelium. Read More

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February 2020