73,204 results match your criteria min injection

Role of hippocampal orexin receptors in antinociception elicited by chemical stimulation of the lateral hypothalamus in the tail-flick test.

Behav Brain Res 2021 Jul 27:113492. Epub 2021 Jul 27.

Neuroscience Research Center, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Electronic address:

The lateral hypothalamus (LH) orexinergic neurons project to numerous brain regions implicated in pain perception, including the CA1 part of the hippocampal formation. Moreover, the roles of orexin receptors (OXRs) in the CA1 in anti-analgesic consequences of the LH chemical stimulation by carbachol, muscarinic receptor agonist, in acute pain have not been clarified. The current research showed OXRs antagonist administration's effect in the CA1 on analgesia elicited by the LH chemical stimulation in a tail-flick test as an acute model of pain. Read More

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Comparison of the effect of electromyogram activity during emergence on anesthetic depth monitoring between phase lag entropy and bispectral index: a prospective observational study.

Ann Palliat Med 2021 Jul 14. Epub 2021 Jul 14.

Department of Anesthesia and Pain Medicine, Pusan National University Yangsan Hospital, Yangsan, Republic of Korea.

Background: The bispectral index (BIS) is the most widely used algorithm for measuring anesthetic depth. The BIS has been demonstrated as inaccurate when neuromuscular blocking drugs (NMBDs) are used. Compared with BIS, phase lag entropy (PLE), which measures the anesthetic depth based on a 4-channel EEG signal, is less affected by EMG. Read More

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Radio-carpal wrist MR arthrography: comparison of ultrasound with fluoroscopy and palpation-guided injections.

Skeletal Radiol 2021 Jul 29. Epub 2021 Jul 29.

The Department of Diagnostic Radiology, Faculty of Medicine, Assiut University, Assiut, Egypt.

Objective: To compare ultrasound- (US), fluoroscopy- (FL), and palpation-guided contrast injection techniques used for dorsal radio-carpal wrist MRA.

Materials And Methods: Patients with chronic wrist pain were randomized as to which injection technique they underwent into three groups of 50 participants. Dorsal radio-carpal contrast injection was performed under US, FL guidance (one radiologist for each), or palpation guidance by an orthopedic surgeon. Read More

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[Preparation and Characterization of Clopidogrel Bisulfate Liposomes].

Sichuan Da Xue Xue Bao Yi Xue Ban 2021 Jul;52(4):630-636

Key Laboratory of Drug-Targeting and Drug Delivery System of the Ministry of Education, West China School of Pharmacy, Sichuan University, Chengdu 610041, China.

Objective: To prepare encapsulated clopidogrel bisulfate (CLP) liposomes so as to deal with the poor water solubility of CLP, and to provide the experimental basis for the development of CLP formulations for intravascular injection.

Methods: CLP-loaded liposomes were prepared using thin film hydration/sonication method and pH gradient active drug loading technology. Then, the morphology, particle size, encapsulation efficiency, drug loading capacity, Zeta potentials and release behavior were characterized. Read More

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Lateral Preoptic Area Neurons Activated by Angiotensin-(1-7) Increase Intravesical Pressure: A Novel Feature in Central Micturition Control.

Front Physiol 2021 12;12:682711. Epub 2021 Jul 12.

Department of Morphology and Physiology, Faculdade de Medicina do ABC, Centro Universitario FMABC, Santo Andre, Brazil.

Central micturition control and urine storage involve a multisynaptic neuronal circuit for the efferent control of the urinary bladder. Electrical stimulation of the lateral preoptic area (LPA) at the level of the decussation of the anterior commissure in cats evokes relaxation of the bladder, whereas ventral stimulation of LPA evokes vigorous contraction. Endogenous Angiotensin-(1-7) [(Ang-(1-7)] synthesis depends on ACE-2, and its actions on binding to Mas receptors, which were found in LPA neurons. Read More

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The ED and ED of Prophylactic Norepinephrine for Preventing Post-Spinal Hypotension During Cesarean Delivery Under Combined Spinal-Epidural Anesthesia: A Prospective Dose-Finding Study.

Front Pharmacol 2021 12;12:691809. Epub 2021 Jul 12.

Department of Anesthesia, Jiaxing University Affiliated Women and Children Hospital, Jiaxing, China.

Hypotension commonly occurs with spinal anesthesia during cesarean delivery. Norepinephrine is an alternative to phenylephrine which can be used to prevent or treat hypotension, with better maintained cardiac output and less bradycardia. However, an appropriate initial prophylactic infusion dose of norepinephrine remains unclear. Read More

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Near-infrared fluorescence imaging with indocyanine green for quantification of changes in tissue perfusion following revascularization.

Vascular 2021 Jul 28:17085381211032826. Epub 2021 Jul 28.

Department of Vascular Surgery, 4501Leiden University Medical Center, Leiden, The Netherlands.

Objectives: Current diagnostic modalities for patients with peripheral artery disease (PAD) mainly focus on the macrovascular level. For assessment of tissue perfusion, near-infrared (NIR) fluorescence imaging using indocyanine green (ICG) seems promising. In this prospective cohort study, ICG NIR fluorescence imaging was performed pre- and post-revascularization to assess changes in foot perfusion. Read More

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Closed Repair Technique: Innovative Surface Design for Polymethylmethacrylate Denture Base Repair.

J Prosthodont 2021 Jul 27. Epub 2021 Jul 27.

Department of Substitutive Dental Sciences, College of Dentistry, Imam Abdulrahman Bin Faisal University, Dammam, Saudi Arabia.

Purpose: This study aimed to evaluate the repair strength of a newly introduced repair technique involving zero-gap repair width.

Methods: A total of 36 rectangular prism specimens with dimensions of 64 × 10 × 3.3 mm were prepared of heat-polymerized acrylic resin. Read More

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Constant-rate intravenous infusion of indocyanine green leading to high fluorescence intensity in infrared thoracoscopic segmentectomy.

JTCVS Tech 2020 Sep 11;3:319-324. Epub 2020 May 11.

Department of General Thoracic Breast and Endocrinological Surgery, Kagawa University, Takamatsu City, Kagawa Prefecture, Japan.

Objectives: The purpose of this study was to determine whether or not fluorescence could be increased by administering indocyanine green at a constant rate, thus stabilizing its blood concentration.

Methods: In 20 consecutive patients undergoing segmentectomy, the dominant pulmonary arteries were ligated, blocking blood in the target segment. Fluorescence intensity was then observed using different indocyanine green administration methods under infrared thoracoscopy. Read More

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September 2020

Biodistribution and internal radiation dosimetry of a companion diagnostic radiopharmaceutical, [Ga]PSMA-11, in subcutaneous prostate cancer xenograft model mice.

Sci Rep 2021 Jul 27;11(1):15263. Epub 2021 Jul 27.

Department of Nuclear Medicine, Seoul National University College of Medicine, Seoul National University Bundang Hospital, 82 Gumi-ro, 173 Beon-gil, Bundang-gu, Seongnam, 13620, Korea.

[Ga]PSMA-11 is a prostate-specific membrane antigen (PSMA)-targeting radiopharmaceutical for diagnostic PET imaging. Its application can be extended to targeted radionuclide therapy (TRT). In this study, we characterize the biodistribution and pharmacokinetics of [Ga]PSMA-11 in PSMA-positive and negative (22Rv1 and PC3, respectively) tumor-bearing mice and subsequently estimated its internal radiation dosimetry via voxel-level dosimetry using a dedicated Monte Carlo simulation to evaluate the absorbed dose in the tumor directly. Read More

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Safety assessment and potential risks of the glucagon stimulation test in the diagnosis of secondary adrenal insufficiency.

Curr Drug Saf 2021 Jul 27. Epub 2021 Jul 27.

University Hospital of Farhat Hached Sousse, Endocrinology, Tunisia.

Background: Although it takes more time, the glucagon stimulation test (GST) is a reliable measure for assessing growth hormone (GH) and adrenocorticotropic hormone (ACTH) secretion. The GST is considered to be a safe test, however, it still has mild side effects and potential risks.

Objective: The objective of this study was to analyze the side effects of the GST while testing adrenal insufficient patients. Read More

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Rosmarinic acid attenuates lipopolysaccharide-induced neuroinflammation and cognitive impairment in rats.

J Chem Neuroanat 2021 Jul 24;117:102008. Epub 2021 Jul 24.

Cellular and Molecular Research Center, Birjand University of Medical Sciences, Birjand 9717853577, Iran; Department of Anatomical Sciences, Faculty of Medicine, Birjand University of Medical Sciences, Birjand 9717853577, Iran. Electronic address:

It has been recently demonstrated that rosmarinic acid (RA) through modulation in the amyloidogenic pathway exhibit neuroprotective potential in Alzheimer's disease. However, its effects on non-amyloidogenic pathways such as neuroinflammation (NI) and oxidative stress have not been elucidated carefully. Hence, this study aimed to investigate the effect of RA on cognitive function, cortical and hippocampal oxidant-antioxidant balance, and proinflammatory cytokines production in lipopolysaccharide (LPS)-induced NI in rats. Read More

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(R)-Ketamine attenuates LPS-induced endotoxin-derived delirium through inhibition of neuroinflammation.

Psychopharmacology (Berl) 2021 Jul 27. Epub 2021 Jul 27.

Division of Clinical Neuroscience, Chiba University Center for Forensic Mental Health, Chiba, 260-8670, Japan.

Rationale: (R)-Ketamine produced beneficial effects in a variety of models of inflammatory diseases, including low dose of bacterial lipopolysaccharide (LPS) (0.5-1.0 mg/kg)-induced endotoxemia. Read More

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Distinctive detection of insulinoma using [F]FB(ePEG12)12-exendin-4 PET/CT.

Sci Rep 2021 Jul 22;11(1):15014. Epub 2021 Jul 22.

Department of Diabetes, Endocrinology and Nutrition, Graduate School of Medicine, Kyoto University, 54 Kawahara-cho, Shogoin, Sakyo-ku, Kyoto, 606-8507, Japan.

Specifying the exact localization of insulinoma remains challenging due to the lack of insulinoma-specific imaging methods. Recently, glucagon-like peptide-1 receptor (GLP-1R)-targeted imaging, especially positron emission tomography (PET), has emerged. Although various radiolabeled GLP-1R agonist exendin-4-based probes with chemical modifications for PET imaging have been investigated, an optimal candidate probe and its scanning protocol remain a necessity. Read More

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Blockade of the orexin 1 receptors in the nucleus accumbens' shell reversed the reductioneffect of olanzapine on motivation for positive reinforcers.

Neurosci Lett 2021 Jul 23:136137. Epub 2021 Jul 23.

Institute for Basic Sciences, Physiology Research Center, Kashan University of Medical Sciences, Kashan, Iran.

Effort-based choice of high reward requires one to decide how much effort to expend for a certain amount of reward. Orexin is a crucial neuropeptide in the physiological aspect especially a variety of affective and cognitive processes. The nucleus accumbens (NAc) is a region of the neural system that serves effort-related high reward choices andthe Orexin 1 receptor (OX1R) is distributed extensively throughout the nucleus accumbens shell (AcbS). Read More

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Immunogenicity and safety of a recombinant fusion protein vaccine (V-01) against coronavirus disease 2019 in healthy adults: a randomized, double-blind, placebo-controlled, phase II trial.

Chin Med J (Engl) 2021 Jul 22. Epub 2021 Jul 22.

Guangdong Provincial Institute of Biological Products and Materia Medica, Guangzhou, Guangdong 510440, China Guangdong Provincial Center for Disease Control and Prevention, Guangzhou, Guangdong 511430, China Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, Hubei 430071, China National Institutes for Food and Drug Control, Beijing 100050, China Gaozhou Center for Disease Control and Prevention, Maoming, Guangdong 525000, China Livzon Bio Inc., Zhuhai, Guangdong 519045, China Key Laboratory of Infection and Immunity, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China Institute for Hepatology, National Clinical Research Center for Infectious Disease, Shenzhen Third People's Hospital, Shenzhen, Guangdong 518112, China.

Background: Innovative coronavirus disease 2019 (COVID-19) vaccines, with elevated global manufacturing capacity, enhanced safety and efficacy, simplified dosing regimens, and distribution that is less cold chain-dependent, are still global imperatives for tackling the ongoing pandemic. A previous phase I trial indicated that the recombinant COVID-19 vaccine (V-01), which contains a fusion protein (IFN-PADRE-RBD-Fc dimer) as its antigen, is safe and well tolerated, capable of inducing rapid and robust immune responses, and warranted further testing in additional clinical trials. Herein, we aimed to assess the immunogenicity and safety of V-01, providing rationales of appropriate dose regimen for further efficacy study. Read More

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Exogenous oxytocin administration improves the testicular blood flow in rams.

Andrologia 2021 Jul 26:e14193. Epub 2021 Jul 26.

Department of Theriogenology, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt.

The current research aimed to examine the effects of single-dose oxytocin administration on testicular blood flow measurements peak systolic velocity (PSV), end-diastolic velocity (EDV), resistive index (RI) and pulsatility index (PI) and plasma steroid (testosterone and oestradiol-17β) concentrations in rams. In the treated group, eight mature Ossimi rams during the breeding season were injected intravenously (iv) with 20 IU oxytocin, while the other eight male rams were administered normal saline (2 ml) iv as a control group. Venous blood samples and testicular blood flow in the left and right testes were examined immediately before (0) and 5, 30, 60 and 120 min after injections. Read More

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Rapid tumor-labeling kinetics with a site-specific near-infrared anti-CEA nanobody in a patient-derived orthotopic xenograft mouse model of colon cancer.

J Surg Oncol 2021 Jul 26. Epub 2021 Jul 26.

Department of Surgery, University of California San Diego, San Diego, California, USA.

Background/objectives: Nanobodies are the smallest biologic antigen-binding fragments derived from camelid-derived antibodies. Nanobodies effect a peak tumor signal within minutes of injection and present a novel opportunity for fluorescence-guided surgery (FGS). The present study demonstrates the efficacy of an anti-CEA nanobody conjugated to near-infrared fluorophore LICOR-IRDye800CW for rapid intraoperative tumor labeling of colon cancer. Read More

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Comparison of the tissue distribution and metabolism of AN1284, a potent anti-inflammatory agent, after subcutaneous and oral administration in mice.

Naunyn Schmiedebergs Arch Pharmacol 2021 Jul 26. Epub 2021 Jul 26.

Institute of Drug Research, School of Pharmacy, The Hebrew University of Jerusalem, Ein Kerem, 9112002, Jerusalem, Israel.

This study is to compare the tissue distribution and metabolism of AN1284 after subcutaneous and oral administration at doses causing maximal reductions in IL-6 in plasma and tissues of mice. Anti-inflammatory activity of AN1284 and its metabolites was detected in lipopolysaccharide (LPS) activated RAW 264.7 macrophages. Read More

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A compact integrated microfluidic oxygenator with high gas exchange efficiency and compatibility for long-lasting endothelialization.

Lab Chip 2021 Jul 26. Epub 2021 Jul 26.

Université Paris-Saclay, CNRS, Centre de Nanosciences et Nanotechnologies C2N, UMR9001, Palaiseau 91120, France.

We have developed and tested a novel microfluidic device for blood oxygenation, which exhibits a large surface area of gas exchange and can support long-term sustainable endothelialization of blood microcapillaries, enhancing its hemocompatibility for clinical applications. The architecture of the parallel stacking of the trilayers is based on a central injection for blood and a lateral injection/output for gas which allows significant reduction in shear stress, promoting sustainable endothelialization since cells can be maintained viable for up to 2 weeks after initial seeding in the blood microchannel network. The circular design of curved blood capillaries allows covering a maximal surface area at 4 inch wafer scale, producing high oxygen uptake and carbon dioxide release in each single unit. Read More

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miR-451 on Myocardial Ischemia-Reperfusion in Rats by Regulating AMPK Signaling Pathway.

Biomed Res Int 2021 6;2021:9933998. Epub 2021 Jul 6.

Department of Cardiac Surgery, Beijing Chaoyang Hospital, Capital Medical University, Beijing 100020, China.

Myocardial infarction is the main cause of death in patients with coronary heart disease. At present, the main method to treat cardiovascular disease is perfusion therapy. Myocardial ischemia-reperfusion will inevitably lead to reperfusion injury, which is also a major problem in the treatment of cardiovascular diseases. Read More

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Additive Value of Dynamic FDOPA PET/CT for Glioma Grading.

Front Med (Lausanne) 2021 9;8:705996. Epub 2021 Jul 9.

Univ Rennes, CLCC Eugène Marquis, INSERM, LTSI - UMR 1099, Rennes, France.

The aim of this study was to assess the value of the FDOPA PET kinetic parameters extracted using full kinetic analysis for tumor grading with neuronavigation-guided biopsies as reference in patients with newly-diagnosed gliomas. Fourteen patients with untreated gliomas were investigated. Twenty minutes of dynamic positron-emission tomography (PET) imaging and a 20-min static image 10 min after injection were reconstructed from a 40-min list-mode acquisition immediately after FDOPA injection. Read More

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Inactivation of Prefrontal Cortex Delays Emergence From Sevoflurane Anesthesia.

Front Syst Neurosci 2021 9;15:690717. Epub 2021 Jul 9.

Department of Anesthesiology, University of Michigan, Ann Arbor, MI, United States.

Studies aimed at investigating brain regions involved in arousal state control have been traditionally limited to subcortical structures. In the current study, we tested the hypothesis that inactivation of prefrontal cortex, but not two subregions within parietal cortex-somatosensory barrel field and medial/lateral parietal association cortex-would suppress arousal, as measured by an increase in anesthetic sensitivity. Male and female Sprague Dawley rats were surgically prepared for recording electroencephalogram and bilateral infusion into prefrontal cortex ( = 13), somatosensory barrel field (N = 10), or medial/lateral parietal association cortex ( = 9). Read More

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Effects of probiotics on pentylenetetrazol-induced convulsions in mice.

Epilepsy Res 2021 Jul 12;176:106723. Epub 2021 Jul 12.

Student Research Committee, Faculty of Pharmacy, Kerman University of Medical Sciences, Kerman, Iran; Department of Toxicology & Pharmacology, Faculty of Pharmacy, Kerman University of Medical Sciences, Kerman, Iran. Electronic address:

Objective: There are some reports of the effect of the gut microbiota on the central nervous system. The aim of this study was to find out the effect of probiotics on pentylenetetrazol (PTZ)-induced convulsions in mice.

Methods: The mice were pretreated with probiotic powder (Lactobacillus acidophilus, Lactobacillus casei, and Bifidobacterium bifidum) suspended in normal saline by intragastric gavage (IG-gavage) for 14 (group 1) or 28 (group 2) days prior to injection of PTZ (90 mg/kg, intraperitoneally). Read More

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Serial cellular events in bone formation initiated by calcium phosphate ceramics.

Acta Biomater 2021 Jul 22. Epub 2021 Jul 22.

Kuros Biosciences BV, Prof. Bronkhorstlaan 10, 3723 MB Bilthoven, The Netherlands; Huipin Yuan's Lab, Sichuan, China. Electronic address:

To better understand the biological mechanisms triggered by osteoinductive materials in vivo, we evaluated the timeline of cellular responses to osteoinductive materials subcutaneously implanted in FVB mice. More F4/80-positive macrophages were present in osteoinductive tri-CaP ceramic (TCP) with submicron surface topography (TCPs) than non-osteoinductive TCP with micron surface topography (TCPb) at week 1. Moreover, TCPs (but not TCPb) significantly enhanced osteoclastogenesis, and induced macrophages to polarize from M1 to M2 in the first week. Read More

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Flow Field Analysis Inside and at the Outlet of the Abrasive Head.

Materials (Basel) 2021 Jul 14;14(14). Epub 2021 Jul 14.

Department of Biological Instrumentation, Institute of Analytical Chemistry of the Czech Academy of Sciences, 60200 Brno, Czech Republic.

This paper focuses on the investigation of a multiphase flow of water, air, and abrasive particles inside and at the outlet of the abrasive head with the help of computational fluid dynamics calculations and measurements. A standard abrasive head with a water nozzle hole diameter of 0.33 mm (0. Read More

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Superparamagnetic Iron Oxide for Identifying Sentinel Lymph Node in Breast Cancer after Neoadjuvant Chemotherapy: Feasibility Study.

J Clin Med 2021 Jul 16;10(14). Epub 2021 Jul 16.

Department of Surgical Oncology, Medical University of Lublin, Radziwiłłowska 13 St., 20-080 Lublin, Poland.

Sentinel lymph node biopsy (SLNB) is a well-established procedure for staging clinically node-negative early breast cancer (BC). Superparamagnetic iron oxide (SPIO) demonstrated efficacy for nodal identification using a magnetic probe after local retroaeroal interstitial injection. Its benefits lie in its flexibility, which is an essential property in the global setting, where access to the isotope is difficult. Read More

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Profiling the Effects of Repetitive Morphine Administration on Motor Behavior in Rats.

Molecules 2021 Jul 19;26(14). Epub 2021 Jul 19.

Medical School, University of Cyprus, Nicosia 1678, Cyprus.

Efficient repetitive clinical use of morphine is limited by its numerous side effects, whereas analgesic tolerance necessitates subsequent increases in morphine dose to achieve adequate levels of analgesia. While many studies focused on analgesic tolerance, the effect of morphine dosing on non-analgesic effects has been overlooked. This study aimed to characterize morphine-induced behavior and the development and progression of morphine-induced behavioral tolerance. Read More

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Single Exposure to the Cathinones MDPV and α-PVP Alters Molecular Markers of Neuroplasticity in the Adult Mouse Brain.

Int J Mol Sci 2021 Jul 9;22(14). Epub 2021 Jul 9.

Department of Pharmacological and Biomolecular Sciences, Università degli Studi di Milano, Via Balzaretti 9, 20133 Milano, Italy.

Synthetic cathinones have gained popularity among young drug users and are widely used in the clandestine market. While the cathinone-induced behavioral profile has been extensively investigated, information on their neuroplastic effects is still rather fragmentary. Accordingly, we have exposed male mice to a single injection of MDPV and α-PVP and sacrificed the animals at different time points (i. Read More

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Paroxetine effects on morphine analgesic tolerance in rats.

Scand J Pain 2021 Jul 26. Epub 2021 Jul 26.

Department of Pharmacology, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Objectives: To alleviate different pain intensities, morphine administration has been extensively used. However, prolonged administration of morphine leads to a progressive decline of its analgesic effect which limits their overall utility. Morphine tolerance is considered as a challenging issue for the treatment of both acute and chronic pain. Read More

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