73 results match your criteria mimic worst

A toolbox for mimicking gastrointestinal conditions in children: Simulated Paediatric Breakfast Media (SPBM) for addressing the variability of gastric contents after typical paediatric breakfasts.

J Pharm Sci 2021 May 19. Epub 2021 May 19.

Department of Pharmacy, University of Greifswald, 17489 Greifswald. Electronic address:

Since co-administration of dosage forms with food can impact drug exposure, food effect studies became an integral part of oral drug product development. Studies are usually performed in healthy adults and the dosage form is co-administered with a high-fat high-calorie standard breakfast meal to mimic worst-case dosing conditions. A corresponding study design for children is lacking but would be essential for a proper risk-assessment in this vulnerable patient group. Read More

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Spotting isomer mixtures in forensic illicit drug casework with GC-VUV using automated coelution detection and spectral deconvolution.

J Chromatogr B Analyt Technol Biomed Life Sci 2021 Mar 29;1173:122675. Epub 2021 Mar 29.

Van 't Hoff Institute for Molecular Sciences, University of Amsterdam, PO Box 94157, Amsterdam 1090 GD, the Netherlands; Co van Ledden Hulsebosch Center (CLHC), Amsterdam Center for Forensic Science and Medicine, PO Box 94157, Amsterdam 1090 GD, the Netherlands.

Analysis of isomeric mixtures is a significant analytical challenge. In the forensic field, for example, over 1000 new psychoactive substances (NPSs), comprising of many closely related and often isomeric varieties, entered the drugs-of-abuse market within the last decade. Unambiguous identification of the isomeric form requires advanced spectroscopic techniques, such as GC-Vacuum Ultraviolet Spectroscopy (GC-VUV). Read More

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New insights into application of nanoparticles in the diagnosis and screening of novel coronavirus (SARS-CoV-2).

Emergent Mater 2021 Mar 31:1-17. Epub 2021 Mar 31.

School of Energy Materials, Mahatma Gandhi University, Kerala, 686560 India.

Novel coronavirus disease 2019 (COVID-19) is by far the worst pandemic disease in the current millennium. The first human-to-human transmission was observed in December 2019 in China and is caused by the highly contagious severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which has infected millions of people within months across the globe. SARS-CoV-2 is a spike protein enveloped virus with particle-like characteristics and a diameter of 60-140 nm. Read More

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The Role of Frozen Section in the Rapid Diagnosis of Severe Cutaneous Adverse Drug Reactions.

Indian Dermatol Online J 2021 Jan-Feb;12(1):78-83. Epub 2021 Jan 16.

Department of Dermatology, Venereology and Leprosy, Christian Medical College, Vellore, Tamil Nadu, India.

Context: Early diagnosis is the mainstay in the management of severe cutaneous adverse reactions (SCARs) to drugs.

Aims: To study the role of frozen section in the rapid diagnosis of SCARs and the impact on outcome of the affected patients.

Settings And Design: A single-blind, hospital-based study was conducted from December 2014-July 2016. Read More

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January 2021

Corrective factors applied to reduced sulfur compounds in wastewater foul air.

Water Environ Res 2021 Mar 17. Epub 2021 Mar 17.

Dept. of Env. Health Sciences, UCLA, Los Angeles, CA, USA.

To determine accurately odorant concentrations at its worst-case condition for planning and odor treatment design purposes, corrective factors need to be factored into the foul air monitoring results of water resource recovery facilities. These corrective factors will adjust each odorant concentration for usual seasonal and daily odor variations. Typically, corrective factors are taken from hydrogen sulfide continuous readings and applied to all identified sulfur odorants. Read More

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Effects of preoperative oral carbohydrate on postoperative discomfort in patients undergoing elective surgery: a meta-analysis of randomized controlled trials.

Langenbecks Arch Surg 2021 Jun 25;406(4):993-1005. Epub 2021 Feb 25.

Cochrane Taiwan, Taipei Medical University, Taipei, Taiwan.

Purpose: Fasting is a standard preoperative procedure performed to prevent vomiting and pulmonary aspiration during anaesthesia and surgery. However, fasting can cause postoperative physical and psychological discomfort. Intake of oral carbohydrate (CHO) may mimic the intake of food, which prevents postoperative discomfort. Read More

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Impact of different radiation techniques and doses on cardiac implantable electronic devices.

Z Med Phys 2021 Jan 28. Epub 2021 Jan 28.

Universitaetsklinikum Muenster, Department of Radiation Oncology, Albert-Schweitzer Campus 1 A, 48149, Muenster, Germany.

Background And Objective: Purpose of this investigation was to get deeper insight into the impact of different radiation techniques and doses on cardiac implantable electric devices (CIEDs). We aimed to mimic a worst-case scenario with very high doses and external radiation being applied directly on the devices.

Methods: Radiation was applied on 21 CIEDs as photon or electron therapy with maximum dose of 150Gy in fractions of 2 -20Gy. Read More

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January 2021

Three-dimensional multicellular cell culture for anti-melanoma drug screening: focus on tumor microenvironment.

Cytotechnology 2021 Feb 20;73(1):35-48. Epub 2020 Nov 20.

Departamento de Ciências Farmacêuticas, GEIMM-Grupo de Estudos de Interações entre Micro e Macromoléculas, Universidade Federal de Santa Catarina, S/N Centro de Ciências da Saúde Bloco H - 3° andar, sala H302-Bairro Trindade, Florianópolis, Santa Catarina CEP: 88040-900 Brazil.

Abstract: The development of new treatments for malignant melanoma, which has the worst prognosis among skin neoplasms, remains a challenge. The tumor microenvironment aids tumor cells to grow and resist to chemotherapeutic treatment. One way to mimic and study the tumor microenvironment is by using three-dimensional (3D) co-culture models (spheroids). Read More

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February 2021

Mechanistic insights into viral clearance during the chromatography steps in antibody processes by using virus surrogates.

Biotechnol Prog 2020 11 21;36(6):e3057. Epub 2020 Aug 21.

Biologics Development, Bristol-Myers Squibb, Devens, Massachusetts, USA.

Viral safety is required for biological products to treat human diseases, and the burden of inactivation and or virus removal lies on the downstream purification process. Minute virus of mice (MVM) is a nonenveloped parvovirus commonly used as the worst-case model virus in validation studies because of its small size and high chemical stability. In this study, we investigated the use of MVM-mock virus particle (MVP) and bacteriophage ΦX174 as surrogates for MVM to mimic viral clearance studies, with a focus on chromatography operations. Read More

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November 2020

Morphological response of the red palm weevil, Rhynchophorus ferrugineus, to a transient low temperature analyzed by computer tomography and holographic microscopy.

J Therm Biol 2020 Dec 7;94:102748. Epub 2020 Oct 7.

Departamento de Física Aplicada, Universidad Miguel Hernández, E3202-Elche, Alicante, Spain.

The red palm weevil (RPW), Rhynchophorus ferrugineus, is one of the worst palm pests worldwide. Our study aims to assess its internal and external morphological response to a sudden but transient decrease in the environmental temperature. Wild pre-pupae were subjected for 7 days to either low (5. Read More

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December 2020

The Open Challenge of Modeling Complex and Multi-Microbial Communities in Three-Dimensional Niches.

Front Bioeng Biotechnol 2020 20;8:539319. Epub 2020 Oct 20.

Department of Chemistry, Materials and Chemical Engineering "Giulio Natta" and UdR INSTM Politecnico di Milano, Milan, Italy.

The comprehension of the underlying mechanisms of the interactions within microbial communities represents a major challenge to be faced to control their outcome. Joint efforts of , and ecological models are crucial to controlling human health, including chronic infections. In a broader perspective, considering that polymicrobial communities are ubiquitous in nature, the understanding of these mechanisms is the groundwork to control and modulate bacterial response to any environmental condition. Read More

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October 2020

ΕGFR/ERβ-Mediated Cell Morphology and Invasion Capacity Are Associated with Matrix Culture Substrates in Breast Cancer.

Cells 2020 10 8;9(10). Epub 2020 Oct 8.

Biochemistry, Biochemical Analysis & Matrix Pathobiology Research Group, Laboratory of Biochemistry, Department of Chemistry, University of Patras, 26504 Patras, Greece.

Breast cancer accounts for almost one in four cancer diagnoses in women. Studies in breast cancer patients have identified several molecular markers, indicators of aggressiveness, which help toward more individual therapeutic approaches. In triple-negative breast cancer (TNBC), epidermal growth factor receptor (EGFR) overexpression is associated with increased metastatic potential and worst survival rates. Read More

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October 2020

MicroRNA 200b promotes mesenchymal-to-epithelial transition in anaplastic thyroid carcinoma.

Oncol Lett 2020 Oct 14;20(4). Epub 2020 Jul 14.

Department of Otolaryngology-Head and Neck Surgery, Wakayama Medical University, Wakayama, Wakayama 641-8509, Japan.

Anaplastic thyroid cancer (ATC) remains a cancer with one of the worst prognoses, despite novel targeted therapies. The median survival rate has not improved for decades. Epithelial-to-mesenchymal transition (EMT) is a crucial step in physiological processes and in cancer progression, but the underlying mechanisms are not yet fully understood. Read More

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October 2020

A statistical shape modeling approach for predicting subject-specific human skull from head surface.

Med Biol Eng Comput 2020 Oct 25;58(10):2355-2373. Epub 2020 Jul 25.

Université de technologie de Compiègne, CNRS, UMR 7338 Biomechanics and Bioengineering, Centre de recherche Royallieu, 60 319, Compiègne, CS, France.

Human skull is an important body structure for jaw movement and facial mimic simulations. Surface head can be reconstructed using 3D scanners in a straightforward way. However, internal skull is challenging to be generated when only external information is available. Read More

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October 2020

Upcoming Revolutionary Paths in Preclinical Modeling of Pancreatic Adenocarcinoma.

Front Oncol 2019 22;9:1443. Epub 2020 Jan 22.

Centre de Recherche en Cancérologie de Marseille (CRCM), INSERM U1068, CNRS UMR 7258, Aix-Marseille Université and Institut Paoli-Calmettes, Parc Scientifique et Technologique de Luminy, Marseille, France.

To date, PDAC remains the cancer having the worst prognosis with mortality rates constantly on the rise. Efficient cures are still absent, despite all attempts to understand the aggressive physiopathology underlying this disease. A major stumbling block is the outdated preclinical modeling strategies applied in assessing effectiveness of novel anticancer therapeutics. Read More

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January 2020

Could 3D models of cancer enhance drug screening?

Biomaterials 2020 02 26;232:119744. Epub 2019 Dec 26.

3B's Research Group, I3Bs - Research Institute on Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, Parque de Ciência e Tecnologia, Zona Industrial da Gandra, 4805-017, Barco, Guimarães, Portugal; ICVS/3B's-PT Government Associate Laboratory, Braga, Guimarães, Portugal. Electronic address:

Cancer is a multifaceted pathology, where cellular and acellular players interact to drive cancer progression and, in the worst-case, metastasis. The current methods to investigate the heterogeneous nature of cancer are inadequate, since they rely on 2D cell cultures and animal models. The cell line-based drug efficacy and toxicity assays are not able to predict the tumor response to anti-cancer agents and it is already widely discussed how molecular pathway are not recapitulated in vitro so called flat biology. Read More

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February 2020

A flow-based platform hyphenated to on-line liquid chromatography for automatic leaching tests of chemical additives from microplastics into seawater.

J Chromatogr A 2019 Sep 20;1602:160-167. Epub 2019 Jun 20.

FI-TRACE Group, Department of Chemistry, University of the Balearic Islands, Carretera de Valldemossa km 7.5, E-07122 Palma, Spain. Electronic address:

An automatic flow-based system as a front end to liquid chromatography (LC) for on-line dynamic leaching of microplastic materials (polyethylene of medium density and poly(vinyl chloride)) with incurred phthalates and bisphenol A is herein presented. The microplastic particles were packed in a metal column holder, through which seawater was pumped continuously by resorting to advanced flow methodology. Each milliliter of the leachable (bioaccessible) fraction of chemical additives was preconcentrated on-line using a 10 mm-long octadecyl monolithic silica column placed in the sampling loop of the injection valve of a HPLC system that served concomitantly for analyte uptake and removal of the seawater matrix. Read More

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September 2019

Mimicking Anti-Viruses with Machine Learning and Entropy Profiles.

Entropy (Basel) 2019 May 21;21(5). Epub 2019 May 21.

Hoffmann-La Roche, 28027 Madrid, Spain.

The quality of anti-virus software relies on simple patterns extracted from binary files. Although these patterns have proven to work on detecting the specifics of software, they are extremely sensitive to concealment strategies, such as polymorphism or metamorphism. These limitations also make anti-virus software predictable, creating a security breach. Read More

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Prognostic accuracy of the serum lactate level, the SOFA score and the qSOFA score for mortality among adults with Sepsis.

Scand J Trauma Resusc Emerg Med 2019 Apr 30;27(1):51. Epub 2019 Apr 30.

Department of Pancreatic Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China.

Background: Sepsis is a common critical condition caused by the body's overwhelming response to certain infective agents. Many biomarkers, including the serum lactate level, have been used for sepsis diagnosis and guiding treatment. Recently, the Third International Consensus Definitions for Sepsis and Septic Shock (Sepsis-3) recommended the Sequential Organ Failure Assessment (SOFA) and the quick SOFA (qSOFA) rather than lactate for screening sepsis and assess prognosis. Read More

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Gap between the fragment and the tibia affects the stability of tibial tubercle osteotomy: A finite element study.

Med Eng Phys 2019 06 8;68:57-64. Epub 2019 Apr 8.

Department of BioMedical Engineering, National Cheng Kung University, Tainan, Taiwan; Metal Industries Research & Development Centre, Kaohsiung, Taiwan. Electronic address:

Tibial tubercle osteotomy (TTO) is commonly performed in cases of complicated juxta-articular trauma or revision total knee arthroplasty. However, strategies for firmly fixing the resulting osteotomy bone fragment are not sufficiently understood. This study aims to investigate the effect of the location of the gap between the fragment and the tibia and with various fixed screw configurations on TTO stability, contact force on the fragment, and bone stress by using the finite element method. Read More

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Gemcitabine enhances OSI-027 cytotoxicity by upregulation of miR-663a in pancreatic ductal adenocarcinoma cells.

Am J Transl Res 2019 15;11(1):473-485. Epub 2019 Jan 15.

Department of Hepatobiliary and Pancreatic Surgery, The First Affiliated Hospital, School of Medicine, Zhejiang University No. 79 Qingchun Road, Hangzhou 310003, China.

Pancreatic ductal adenocarcinoma (PDAC) is well-known to be the most deadly malignancy with the worst survival rate of all cancers. Gemcitabine-based chemotherapy is the most common treatment option for pancreatic ductal adenocarcinoma. However, it offers little therapeutic value in many cases due to the rapid development of chemoresistance. Read More

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January 2019

Detector for monitoring potential bleeding during electron intraoperative radiotherapy.

Phys Med 2019 Jan 3;57:95-99. Epub 2019 Jan 3.

IRIMED, Unidad Mixta de Investigacion en Radiofisica e Instrumentacion Nuclear en Medicina IIS Hospital La Fe - Universitat de Valencia, Spain; Department of Radiation Oncology, La Fe Polytechnic and University Hospital, Valencia E-46026, Spain.

Purpose: The aim of this work is to develop a bleeding detector integrated into the acrylic circular applicators for specific mobile linacs. Thus, a bleeding detector has been developed based on a capacitive sensor to be used with plastic applicators, as in the case of LIAC HWL from Sordina IORT Technologies SpA. According to the clinical impact, we have selected 0. Read More

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January 2019

Variable expressivity of HJV related hemochromatosis: "Juvenile" hemochromatosis?

Blood Cells Mol Dis 2019 02 22;74:30-33. Epub 2018 Oct 22.

Univ Rennes, CHU Rennes, INSERM, French Reference Center for Hemochromatosis and Iron Metabolism Disease, F-35000 Rennes, France; Univ Rennes, CHU Rennes, INSERM, Liver Disease Department, F-35000 Rennes, France.

Juvenile hemochromatosis is a rare autosomal recessive disease due to variants in the Hemojuvelin (HJV) gene. Although biological features mimic HFE hemochromatosis, clinical presentation is worst with massive iron overload diagnosed during childhood. Our study describes clinical features and results of genetic testing for a group of patients initially referred for a hepcidino-deficiency syndrome and for whom HJV hemochromatosis was finally diagnosed. Read More

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February 2019

Caval to pulmonary 3D flow distribution in patients with Fontan circulation and impact of potential 4D flow MRI error sources.

Magn Reson Med 2019 02 15;81(2):1205-1218. Epub 2018 Sep 15.

Department of Radiology, Feinberg School of Medicine, Northwestern University, Chicago, Illinois.

Purpose: Uneven flow distribution in patients with Fontan circulation is suspected to lead to complications. 4D flow MRI offers evaluation using time-resolved pathlines; however, the potential error is not well understood. The aim of this study was to systematically assess variability in flow distribution caused by well-known sources of error. Read More

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February 2019

Traumatic osteoarthritis-induced persistent mechanical hyperalgesia in a rat model of anterior cruciate ligament transection plus a medial meniscectomy.

J Pain Res 2018 22;11:41-50. Epub 2017 Dec 22.

Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei, Taiwan.

Background: Osteoarthritis (OA) is a degenerative joint disease characterized by progressive cartilage degeneration, subchondral bone changes, osteophyte formation, and synovitis. A major symptom is pain that is triggered by peripheral and central changes within the pain pathways. Some surgery-induced joint instability rat models of OA were described to mimic traumatic OA. Read More

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December 2017

High density marker panels, SNPs prioritizing and accuracy of genomic selection.

BMC Genet 2018 01 5;19(1). Epub 2018 Jan 5.

Department of Animal and Dairy Science, University of Georgia, Athens, GA, 30602, USA.

Background: The availability of high-density (HD) marker panels, genome wide variants and sequence data creates an unprecedented opportunity to dissect the genetic basis of complex traits, enhance genomic selection (GS) and identify causal variants of disease. The disproportional increase in the number of parameters in the genetic association model compared to the number of phenotypes has led to further deterioration in statistical power and an increase in co-linearity and false positive rates. At best, HD panels do not significantly improve GS accuracy and, at worst, reduce accuracy. Read More

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January 2018

[Posterior fossa infarct misdiagnosed as acute peripheral vestibulopathy].

Ugeskr Laeger 2018 01;180(1)

Patients with acute central vestibular syndrome (AVS) can mimic acute peripheral vestibulopathy, which can mislead to a diagnosis of posterior fossa infarcts. Delayed diagnosis will prevent relevant treatment and may lead to severe disability and in worst case death. Understanding of AVS is extremely relevant for physicians in hospital and prehospital care to insure the right treatment. Read More

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January 2018

Cerebral venous thrombosis as a diagnostic challenge: Clinical and radiological correlation based on the retrospective analysis of own cases.

Adv Clin Exp Med 2017 Oct;26(7):1113-1122

Department of Neurology, Wroclaw Medical University, Poland.

Background: Cerebral venous thrombosis (CVT) is a rare condition which constitutes 0.5-1% of all strokes. The clinical and radiological picture of CVT is non-specific and can mimic other disorders. Read More

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October 2017

Comparative study of a novel application of automated HR HPV assay and stability in a previously untested Preservative media.

Papillomavirus Res 2017 12 4;4:12-16. Epub 2017 Jun 4.

Lab21 Ltd., Park House, Winship Road, Cambridge CB24 6BQ, United Kingdom.

Background: The suitability and stability of cervical cells in Novaprep media (NHQ) for certain HPV assays is unknown.

Methods: We evaluated the accuracy of an automated HPV assay (Abbott RealTime HR HPV) for cervical cells prepared in NHQ and NHQ with a pre-treatment to mimic a worst case clinical use, compared to the assay manufacturers media; repeatability and reproducibility of HPV results and the stability of detectable HPV in NHQ over time compared to CE marked liquid based cytology preservatives. Cell lines were used to simulate patient samples. Read More

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December 2017

A comparative study of rank aggregation methods for partial and top ranked lists in genomic applications.

Brief Bioinform 2019 01;20(1):178-189

Department of Clinical Sciences, University of Texas Southwestern Medical Center, Dallas, TX.

Rank aggregation (RA), the process of combining multiple ranked lists into a single ranking, has played an important role in integrating information from individual genomic studies that address the same biological question. In previous research, attention has been focused on aggregating full lists. However, partial and/or top ranked lists are prevalent because of the great heterogeneity of genomic studies and limited resources for follow-up investigation. Read More

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January 2019