81,584 results match your criteria mimic

Design of artificial metalloenzymes for the reduction of nicotinamide cofactors.

J Inorg Biochem 2021 Apr 2;220:111446. Epub 2021 Apr 2.

Sustainable Process Technologies, Faculty of Engineering, University of Nottingham, Nottingham, United Kingdom. Electronic address:

Artificial metalloenzymes result from the insertion of a catalytically active metal complex into a biological scaffold, generally a protein devoid of other catalytic functionalities. As such, their design requires efforts to engineer substrate binding, in addition to accommodating the artificial catalyst. Here we constructed and characterised artificial metalloenzymes using alcohol dehydrogenase as starting point, an enzyme which has both a cofactor and a substrate binding pocket. Read More

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Scenario analysis of COVID-19 transmission dynamics in Malaysia with the possibility of reinfection and limited medical resources scenarios.

Comput Biol Med 2021 Apr 7;133:104372. Epub 2021 Apr 7.

Nano-Optoelectronics Research and Technology Lab (NORLab), School of Physics, Universiti Sains Malaysia, 11800 USM, Penang, Malaysia.

COVID-19 is a major health threat across the globe, which causes severe acute respiratory syndrome (SARS), and it is highly contagious with significant mortality. In this study, we conduct a scenario analysis for COVID-19 in Malaysia using a simple universality class of the SIR system and extensions thereof (i.e. Read More

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Human pluripotent stem cells: an alternative for 3D in vitro modelling of skin disease.

Exp Dermatol 2021 Apr 17. Epub 2021 Apr 17.

Department of Women and Children's Health, School of Life Course Sciences, Faculty of Life Sciences and Medicine, King's College London, London, UK.

To effectively study the skin and its pathology, various platforms have been used to date, with in vitro 3D skin models being considered the future gold standard. These models have generally been engineered from primary cell lines. However, their short life span leading to the use of various donors, imposes issues with genetic variation. Read More

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Functional rice with tandemly repeated Cbl-b ubiquitin ligase inhibitory pentapeptide prevents denervation-induced muscle atrophy in vivo.

Biosci Biotechnol Biochem 2021 Apr 17. Epub 2021 Apr 17.

Faculty of Life and Environmental Science, Shimane University, Matsue, Shimane 690-8504, Japan.

Ubiquitin ligase Cbl-b play a critical role in non-loading-mediated skeletal muscle atrophy: Cbl-b ubiquitinates insulin receptor substrate-1 (IRS-1), an important insulin-like growth factor-1 signaling intermediate molecule, leading to its degradation and a resulting loss in muscle mass. We reported that intramuscular injection of a pentapeptide, DGpYMP, which acts as a mimic of the phosphorylation site in IRS-1, significantly inhibited denervation-induced skeletal muscle loss. In order to explore the possibility of the prevention of muscle atrophy by diet therapy, we examined the effects of oral administration of transgenic rice containing Cblin (Cbl-b inhibitor) peptide (DGYMP) on denervation-induced muscle mass loss in frogs. Read More

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Loss of microRNA-147 function alleviates synovial inflammation through ZNF148 in rheumatoid and experimental arthritis.

Eur J Immunol 2021 Apr 17. Epub 2021 Apr 17.

Institute of Molecular and Translational Medicine (IMTM), and Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Xi'an Jiaotong University Health Science Center, West Yanta Road No.76, Xi'an, Shaanxi, 710061, PR China.

MicroRNA-147 (miR-147) had been previously found induced in synoviocytes by inflammatory stimuli derived from T cells in experimental arthritis. This study was designed to verify whether loss of its function might alleviate inflammatory events in joints of experimental and rheumatoid arthritis (RA). DA rats were injected intradermally with pristane to induce arthritis, and rno-miR-147 antagomir was locally administrated into individual ankle compared with negative control or rno-miR-155-5p antagomir (potential positive control). Read More

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Synthetic Routes to Single Chain Polymer Nanoparticles (SCNPs): Current Status and Perspectives.

Macromol Rapid Commun 2021 Apr 17:e2100035. Epub 2021 Apr 17.

Department of Chemistry, University of Warwick, Coventry, CV4 7AL, UK.

Recent advances in polymer science make it possible to create single chain polymer nanoparticles (SCNPs), which can mimic the folding of natural macromolecules, such as protein and nucleic acid, in terms of their native and functional state. Even though considerable progress has been done during the last years, the synthesis of relatively controlled SCNPs with a good folding accuracy is still challenging due to lack of appropriate chemical synthesis techniques. Different types of SCNPs are developed with enhanced properties and used for various applications, e. Read More

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A prognostic dynamic model applicable to infectious diseases providing easily visualized guides: a case study of COVID-19 in the UK.

Sci Rep 2021 Apr 16;11(1):8412. Epub 2021 Apr 16.

Department of Pharmacology, University of Oxford, Oxford, OX1 3QT, UK.

A reasonable prediction of infectious diseases' transmission process under different disease control strategies is an important reference point for policy makers. Here we established a dynamic transmission model via Python and realized comprehensive regulation of disease control measures. We classified government interventions into three categories and introduced three parameters as descriptions for the key points in disease control, these being intraregional growth rate, interregional communication rate, and detection rate of infectors. Read More

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Enhanced influenza A H1N1 T cell epitope recognition and cross-reactivity to protein-O-mannosyltransferase 1 in Pandemrix-associated narcolepsy type 1.

Nat Commun 2021 04 16;12(1):2283. Epub 2021 Apr 16.

Clinicum, University of Helsinki, Helsinki, Finland.

Narcolepsy type 1 (NT1) is a chronic neurological disorder having a strong association with HLA-DQB1*0602, thereby suggesting an immunological origin. Increased risk of NT1 has been reported among children or adolescents vaccinated with AS03 adjuvant-supplemented pandemic H1N1 influenza A vaccine, Pandemrix. Here we show that pediatric Pandemrix-associated NT1 patients have enhanced T-cell immunity against the viral epitopes, neuraminidase 175-189 (NA) and nucleoprotein 214-228 (NP), but also respond to a NA-mimic, brain self-epitope, protein-O-mannosyltransferase 1 (POMT1). Read More

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Nipple Engineering: Maintaining Nipple Geometry with Externally Scaffolded Processed Autologous Costal Cartilage.

J Plast Reconstr Aesthet Surg 2021 Mar 24. Epub 2021 Mar 24.

Laboratory of Bioregenerative Medicine & Surgery, Division of Plastic Surgery, Weill Cornell Medicine, New York, NY, United States of America; Nancy E. and Peter C. Meinig School of Biomedical Engineering, Cornell University, Ithaca, NY, United States of America. Electronic address:

Introduction: Nipple reconstruction is the essential last step of breast reconstruction after total mastectomy, resulting in improved general and aesthetic satisfaction. However, most techniques are limited by secondary scar contracture and loss of neo-nipple projection leading to patient dissatisfaction. Approximately, 16,000 patients undergo autologous flap breast reconstruction annually, during which the excised costal cartilage (CC) is discarded. Read More

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miR-6858 plays a key role in the process of melatonin inhibition of the malignant biological behavior of glioma.

J Clin Neurosci 2021 May 20;87:137-146. Epub 2021 Mar 20.

Department of Neurosurgery, Qilu Hospital of Shandong University and Institute of Brain and Brain-Inspired Science, Cheeloo College of Medicine, Shandong University, PR China; Shandong Key Laboratory of Brain Function Remodeling, PR China. Electronic address:

MicroRNAs (miRNAs), small non-coding RNA molecules with a length of 18-25 nucleotides, have been shown to be involved in mediating various malignant properties of GBM, including growth, invasion and angiogenesis. Here, we investigated whether miRNAs might be involved in mediating the suppression of malignant properties of GBM by melatonin (MEL), an amine hormone secreted by the pineal gland. Sequencing was performed to screen specifically for miRNAs induced by MEL in U87 and an orthotopically xenografted primary GBM cell line, GBM#P3. Read More

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Individual quality of life and the environment - towards a concept of livable areas for persons with disabilities in Poland.

BMC Public Health 2021 Apr 17;21(1):740. Epub 2021 Apr 17.

SGH Warsaw School of Economics, Institute of Statistics and Demography, Warsaw, Poland.

Background: The United Nations Convention on the Rights of Persons with Disabilities [1] highlights the need to create proper socioeconomic and political conditions for persons with disabilities, with a special focus on their immediate living conditions. According to the Convention, these conditions should be built to ensure that persons with disabilities have the potential to enjoy a high quality of life (QoL), and this principle is reflected in the notion of livable areas. The crucial aspect of this framework is the relationship between the individual QoL and the environment, broadly understood as the socioeconomic as well as the technical conditions in which persons with disabilities function. Read More

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Small extracellular ring domain is necessary for CD82/KAI1'anti-metastasis function.

Biochem Biophys Res Commun 2021 Apr 13;557:110-116. Epub 2021 Apr 13.

Department of Biochemistry and Molecular Biology, Dalian Medical University, Dalian, 116044, China. Electronic address:

The peptide mimicking small extracellular loop of CD82/KAI1 has been reported to inhibit tumor cell migration and metastasis. This provides an evidence that small extracellular loop domain should be important for the function of CD82/KAI1. In this paper, to investigate the structure basis for the function of EC1 mimic peptide, we systematically analyzed the effects of each amino acid residue in EC1 mimic peptide on its bioactivity. Read More

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Long non-coding RNA GAS5 regulates Th17/Treg imbalance in childhood pneumonia by targeting miR-217/STAT5.

Cell Immunol 2021 Apr 1;364:104357. Epub 2021 Apr 1.

Department of Respiratory and Critical Care Medicine, The First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150001, China. Electronic address:

The imbalance of helper T (Th) 17 and regulatory T (Treg) cells plays an important role in the pathogenesis of pneumonia. This study aims to investigate the role and mechanism of long non-coding RNA growth arrest-specific 5 (GAS5) in the differentiation of Th17 cells and Tregs in childhood pneumonia. Expression of GAS5, miR-217, signal transducer and activator of transcription-5 (STAT5), receptor-related orphan receptor γt (RORγt), and transcription factor Forkhead box P3 (Foxp3) were examined by qRT-PCR and western blot. Read More

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Decoys as potential therapeutic tools for diabetes.

Drug Discov Today 2021 Apr 13. Epub 2021 Apr 13.

Biotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran; Applied Biomedical Research Center, Mashhad University of Medical Sciences, Mashhad, Iran; Polish Mother's Memorial Hospital Research Institute (PMMHRI), Lodz, Poland; School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran. Electronic address:

Current therapeutic approaches for diabetes are focused on improving glycemic control to prevent diabetes-related complications, but such approached are not completely successful. Decoy technologies such as decoy oligodeoxynucleotides (ODNs) and decoy peptides have emerged as therapeutic tools in diabetes. Decoy ODNs carry a DNA recognition motif for the binding of transcription factors in order to trap them and block their effects, whereas decoy peptides mimic the binding structure of the receptor protein, bind to the docking site of the target ligand, and prevent the interaction of the ligand and receptor. Read More

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Aspiration Revisited: Prospective Evaluation of a Physiologically Pressurized Model With Animal Correlation and Broader Applicability to Filler Complications.

Aesthet Surg J 2021 Apr 16. Epub 2021 Apr 16.

Background: Aspiration testing before filler injection is controversial. Some believe that aspiration can help prevent inadvertent intravascular injection, while others cite false-negative results and question its value given that the needle position always changes somewhat during injection procedures.

Objectives: To test the relation of false-negative results to the viscosity of the material within the needle lumen and determine whether a less viscous material within the needle lumen could decrease the incidence of false-negative results. Read More

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In Vivo and Ex Vivo Patient-Derived Tumor Xenograft Models of Lymphoma for Drug Discovery.

Curr Protoc 2021 Apr;1(4):e96

Department of Pathology and Laboratory Medicine, Weill Cornell Medicine, New York, New York.

In the hemato-oncology field, remarkable scientific progress has been achieved, primarily propelled by the discovery of new technologies, improvement in genomics, and novel in vitro and in vivo models. The establishment of multiple cell line collections and the development of instrumental mouse models enhanced our ability to discover effective therapeutics. However, cancer models that faithfully mimic individual cancers are still imperfect. Read More

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Carbon-nitrogen conjugate-composited CuS with enhanced peroxidase-like activity for the colorimetric detection of hydrogen peroxide and glutathione.

Anal Methods 2021 Apr 22;13(14):1706-1714. Epub 2021 Mar 22.

Key Laboratory for Colloid & Interface Chemistry of Education Ministry, School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, China.

In this work, cystine-glucose Maillard conjugates were composited with CuS microspheres (CuS-cgmc) to achieve higher sensitivity for the colorimetric analysis. Enhanced peroxidase-like activity was obtained in CuS-cgmc with a carbon elemental ratio of 1.83% compared to bare CuS. Read More

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How Can We Differentiate Local Recurrence From Heterotopic Ossification After Resection and Implantation of an Oncologic Knee Prosthesis in Patients with a Bone Sarcoma?

Clin Orthop Relat Res 2021 May;479(5):1134-1143

K. Jamshidi, A. Bagherifard, A. Mirzaei, Bone and Joint Reconstruction Research Center, Shafa Orthopedic Hospital, Iran University of Medical Sciences, Tehran, Iran.

Background: Heterotopic ossification (HO) is common after total joint arthroplasty and usually does not cause diagnostic problems. However, the occurrence of HO after oncologic prostheses implantation can be troublesome as it may mimic a locally recurrent tumor. Because this distinction could have a profound impact on the surgeon and patient, it is important to distinguish the two entities; to our knowledge, no study has evaluated this after oncologic endoprosthetic reconstruction around the knee after tumor resection. Read More

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Formulation development of a live attenuated human rotavirus (RV3-BB) vaccine candidate for use in low- and middle-income countries.

Hum Vaccin Immunother 2021 Apr 16:1-13. Epub 2021 Apr 16.

Department of Pharmaceutical Chemistry, Vaccine Analytics and Formulation Center, University of Kansas, Lawrence, KS, USA.

Formulation development was performed with the live, attenuated, human neonatal rotavirus vaccine candidate (RV3-BB) with three main objectives to facilitate use in low- and middle- income countries including (1) a liquid, 2-8°C stable vaccine, (2) no necessity for pre-neutralization of gastric acid prior to oral administration of a small-volume dose, and (3) a low-cost vaccine dosage form. Implementation of a high-throughput RT-qPCR viral infectivity assay for RV3-BB, which correlated well with traditional FFA assays in terms of monitoring RV3-BB stability profiles, enabled more rapid and comprehensive formulation development studies. A wide variety of different classes and types of pharmaceutical excipients were screened for their ability to stabilize RV3-BB during exposure to elevated temperatures, freeze-thaw and agitation stresses. Read More

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Epigallocatechin-3-gallate inhibits the growth of three-dimensional in vitro models of neuroblastoma cell SH-SY5Y.

Mol Cell Biochem 2021 Apr 16. Epub 2021 Apr 16.

Target Discovery Institute, Nuffield Department of Medicine, University of Oxford, Oxford, England, UK.

The aim of the study is to investigate the potential of using three-dimensional (3D) in vitro neuroblastoma models to mimic the neuroblastoma microenvironment by testing a potential therapeutic compound-the natural extract epigallocatechin-3-gallate (EGCG), and to further elucidate the roles of DYRK1A in the growth and differentiation of neuroblastoma tissue. In vitro models based on a classic neuroblastoma cell line SH-SY5Y were employed, including 3D models with extracellular matrix and co-cultured with vascular endothelial cells. Cell viability was tested using AlamarBlue and Resazurin assay. Read More

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Serum microRNA-30d is a sensitive biomarker for angiotensin II-induced cardiovascular complications in rats.

Heart Vessels 2021 Apr 16. Epub 2021 Apr 16.

Department of Pathophysiology, Oita University School of Medicine, Yufu, Oita, 879-5593, Japan.

We tested the hypothesis that angiotensin II (Ang II)-induced cardiovascular complications are distinguished from what catecholamine-induced by their serum circulating biomarkers in rats. Infusion of Ang II (1.68 mg/kg/day) significantly increased systolic and diastolic blood pressure assessed at week one or later, accompanied by an increase of heart/body weight ratio. Read More

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Strigolactone mimic 2-nitrodebranone is highly active in Arabidopsis growth and development.

Plant J 2021 Apr 15. Epub 2021 Apr 15.

Shenzhen Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, 518124, China.

Strigolactones play crucial roles in regulating plant architecture and development as endogenous hormones and orchestrating symbiotic interactions with fungi and parasitic plants as components of root exudates. rac-GR24 is currently the most widely used strigolactone analog and serves as a reference compound in investigating the action of strigolactones. In this study, we evaluated a suite of debranones and found that 2-nitrodebranone (2NOD) exhibited higher biological activity than rac-GR24 in various aspects of plant growth and development in Arabidopsis, including hypocotyl elongation inhibition, root hair promotion, and senescence acceleration. Read More

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Single and combined toxicity of amino-functionalized polystyrene nanoparticles with potassium dichromate and copper sulfate on brine shrimp Artemia franciscana larvae.

Environ Sci Pollut Res Int 2021 Apr 16. Epub 2021 Apr 16.

Laboratório de Biologia Celular e do Desenvolvimento (LABID), Departamento de Biologia Molecular (DBM), Centro de Ciências Exatas e da Natureza, Universidade Federal da Paraíba (UFPB, Campus I), Cidade Universitária s/n, Castelo Branco, CEP, João Pessoa, PB, 58051-900, Brazil.

The increasing use and disposal of plastics has become a persistent problem in the marine environment, calling for studies that refer to realistic scenarios to understand their effects on biota. Particularly, the understanding about the effects of co-exposure with nanoplastic particles and metals on aquatic organisms is still limited. The present work aimed to investigate the acute toxicity of amino-functionalized polystyrene nanoparticles (PS-NH; 50 nm) as proxy for nanoplastics on brine shrimp Artemia franciscana larvae under different culture conditions and at different stages of development, as well as the combined effect with two reference toxicants - potassium dichromate (KCrO) and copper sulfate (CuSO). Read More

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Machine learning applied to a Cardiac Surgery Recovery Unit and to a Coronary Care Unit for mortality prediction.

J Clin Monit Comput 2021 Apr 15. Epub 2021 Apr 15.

Department of Anesthesiology, Complejo Hospitalario Universitario de Pontevedra, Mourente s/n, 36071, Pontevedra, Spain.

Most established severity-of-illness systems used for prediction of intensive care unit (ICU) mortality were developed targeted at the general ICU population, based on logistic regression (LR). To date, no dynamic predictive tool for ICU mortality has been developed targeted at the Cardiac Surgery Recovery Unit (CSRU) and Coronary Care Unit (CCU) using machine learning (ML). CSRU and CCU adult patients from the MIMIC-III critical care database were studied. Read More

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Clinical Presentation and Management Strategies in Intraorbital Foreign Bodies.

Case Rep Ophthalmol Med 2021 30;2021:6645952. Epub 2021 Mar 30.

Guru Nanak Eye Centre, Maulana Azad Medical College, New Delhi, India.

The authors present a retrospective, observational case study of seven patients, who presented with retained Intra-Orbital Foreign Bodies (IOrbFBs) following penetrating orbital injury at a tertiary eye hospital over a period of one year. Cases were reviewed for epidemiological features, mechanism of injury, nature of foreign body, clinical features, imaging modality, associated complications, management outcomes, and the final prognosis. The mean age of presentation was 27. Read More

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Airway Epithelial Inflammation Augments the Rescue of Mutant CFTR by Current CFTR Modulator Therapies.

Front Pharmacol 2021 30;12:628722. Epub 2021 Mar 30.

Marsico Lung Institute and Cystic Fibrosis Research Center, School of Medicine, University of North Carolina, Chapel Hill, NC, United States.

In cystic fibrosis (CF), defective biogenesis and activity of the cystic fibrosis transmembrane conductance regulator (CFTR) leads to airway dehydration and impaired mucociliary clearance, resulting in chronic airway infection and inflammation. The most common CFTR mutation, F508del, results in a processing defect in which the protein is retained in the endoplasmic reticulum and does not reach the apical surface. CFTR corrector compounds address this processing defect to promote mutant CFTR transfer to the apical membrane. Read More

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An optimised GAS-pharyngeal cell biofilm model.

Sci Rep 2021 Apr 15;11(1):8200. Epub 2021 Apr 15.

Illawarra Health and Medical Research Institute, Wollongong, NSW, Australia.

Group A Streptococcus (GAS) causes 700 million infections and accounts for half a million deaths per year. Biofilm formation has been implicated in both pharyngeal and dermal GAS infections. In vitro, plate-based assays have shown that several GAS M-types form biofilms, and multiple GAS virulence factors have been linked to biofilm formation. Read More

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Study of cuffless blood pressure estimation method based on multiple physiological parameters.

Physiol Meas 2021 Apr 15. Epub 2021 Apr 15.

College of Biomedical Engineering and Instrument Science, Zhejiang University, Zhejiang Province ZheDa road 38#, Hangzhou, Zhejiang, 310027, CHINA.

Objective: Non-invasive blood pressure measurement technologies have been widely studied, but they still have the disadvantages of low accuracy, require frequent calibration and limited subjects. This work considers the regulation of vascular activity by the sympathetic nervous system and proposes a method for estimating blood pressure using multiple physiological parameters.

Approach: The parameters used in the model consist Heart Rate Variability(HRV), pulse transit time(PTT) and pulse wave morphology features extracted from ECG and PPG signals. Read More

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Textile-based sandwich scaffold using wet electrospun yarns for skin tissue engineering.

J Mech Behav Biomed Mater 2021 Apr 8;119:104499. Epub 2021 Apr 8.

School of Materials Science and Engineering, Georgia Institute of Technology, Atlanta, GA, USA; Georgia Tech Manufacturing Institute, Georgia Institute of Technology, Atlanta, GA, USA; H. Milton Stewart School of Industrial and Systems Engineering, Georgia Institute of Technology, Atlanta, GA, USA.

One of the key elements in tissue engineering is to design and fabricate scaffolds with tissue-like properties. However, mimicking the strain-stiffening property of human tissues by using synthetic materials is still a challenge in scaffold fabrication since most synthetic materials exhibit strain-softening behavior. To address this challenge, we propose a textile-based sandwich scaffold to mimic strain-stiffening behavior observed in human tissues. Read More

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Human umbilical cord mesenchymal stem cell-derived small extracellular vesicles ameliorate collagen-induced arthritis via immunomodulatory T lymphocytes.

Mol Immunol 2021 Apr 12;135:36-44. Epub 2021 Apr 12.

Department of Rheumatology, Shanxi Bethune Hospital, Shanxi Academy of Medical Sciences, Shanxi Bethune Hospital Affiliated to Shanxi Medical University, Taiyuan, 030032, China. Electronic address:

Background: Rheumatoid arthritis (RA) is an autoimmune disease for which there are currently no effective therapies. Although mesenchymal stem cells (MSCs) can prevent arthritis through immunomodulatory mechanisms, there are several associated risks. Alternatively, MSC-derived small extracellular vesicles (sEVs) can mimic the effects of MSCs, while reducing the risk of adverse events. Read More

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