59 results match your criteria midguts treated

Behavioral and ultrastructural effects of novaluron on Aedes aegypti larvae.

Infect Genet Evol 2021 09 21;93:104974. Epub 2021 Jun 21.

Department of General Biology, Federal University of Viçosa, 36570-000 Viçosa, MG, Brazil. Electronic address:

Chitin synthesis inhibitors (CSI) are supposed to inhibit formation of chitin microfibrils in newly synthesized cuticle during molting process. Conversely, there has been comparatively few data on morphological effects of CSI on non-target insect organs. In this work, the effects of the CSI novaluron on behavior and midgut of A. Read More

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September 2021

Impact of Protoporphyrin Lysine Derivatives on the Ability of Spores to Infect Honeybees.

Insects 2020 Aug 5;11(8). Epub 2020 Aug 5.

Department of Industrial and Environmental Microbiology, Faculty of Biology and Biotechnology, Maria Curie-Skłodowska University, Akademicka 19, 20-033 Lublin, Poland.

The effect of two protoporphyrin IX derivatives conjugated with single (PP[Lys(TFA)-OH)]) or double (PP[Lys(TFA)-Lys(TFA)-OH]) lysine moieties on the infectious capacity of spores was examined, and their efficacies were compared with those of a cationic porphyrin (HTTMePP). Honeybees were inoculated with spores preincubated with porphyrins or with untreated spores (control). A significantly lower level of infection was observed in the bees infected with the porphyrin-treated spores than in the infected control. Read More

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Effects of sublethal phoxim exposure and lower food intake on nutrient metabolism in the midguts of Bombyx mori.

Pestic Biochem Physiol 2020 Jul 22;167:104593. Epub 2020 Apr 22.

School of Basic Medicine and Biological Sciences, Soochow University, Suzhou, Jiangsu 215123, PR China; Sericulture Institute of Soochow University, Soochow University, Suzhou, Jiangsu 215123, PR China. Electronic address:

Silkworm (Bombyx mori) is an economically important insect. However, the survival of silkworms has been significantly affected by the assault of chemical pesticides on mulberry trees through aerial application and water currents. Phoxim is a broad-spectrum organophosphorus insecticide widely used in China. Read More

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A method for purification of Plasmodium oocysts from mosquito midguts.

Sci Rep 2020 04 29;10(1):7262. Epub 2020 Apr 29.

Institute of molecular biology and biotechnology, Foundation for research and technology - Hellas, Heraklion, Greece.

Malaria parasites have a complex life cycle comprising development in two hosts, the vertebrate and the vector mosquito. In the gut of the mosquito, the parasite develops into the oocyst, which is settled beneath the epithelium and attached to the basal lamina of the gut until the maturation of the cyst and its rupture concomitant with the release of the sporozoites, the infectious form of the parasite. The oocyst represents the longest stage of the parasite life cycle but it is poorly understood, mainly because of the difficulties to separate the oocysts from the mosquito midgut tissue but also the lack of a robust method to reproduce this stage in vitro. Read More

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NPC1161B, an 8-Aminoquinoline Analog, Is Metabolized in the Mosquito and Inhibits Oocyst Maturation.

Front Pharmacol 2019 25;10:1265. Epub 2019 Oct 25.

W. Harry Feinstone Department of Molecular Microbiology and Immunology, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD, United States.

Malaria is a major global health threat, with nearly half the world's population at risk of infection. Given the recently described delayed clearance of parasites by artemisinin-combined therapies, new antimalarials are needed to facilitate the global effort toward elimination and eradication. NPC1161 is an 8-aminoquinoline that is derived from primaquine with an improved therapeutic profile compared to the parent compound. Read More

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October 2019

Exposure of mosquito (Aedes aegypti) larvae to the water extract and lectin-rich fraction of Moringa oleifera seeds impairs their development and future fecundity.

Ecotoxicol Environ Saf 2019 Nov 22;183:109583. Epub 2019 Aug 22.

Departamento de Bioquímica, Centro de Biociências, Universidade Federal de Pernambuco, Recife, Pernambuco, Brazil. Electronic address:

Aedes aegypti control is a key component of the prophylaxis of dengue fever and other diseases. Moringa oleifera seeds contain a water-soluble lectin (WSMoL) with larvicidal and ovicidal activities against this insect. In this study, A. Read More

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November 2019

Cu/ZnSOD always responded stronger and rapider than MnSOD in Lymantria dispar larvae under the avermectin stress.

Pestic Biochem Physiol 2019 May 11;156:72-79. Epub 2019 Feb 11.

School of Forest, Northeast Forestry University, Harbin 150040, China. Electronic address:

Metalloenzyme SODs play important roles in insects dealing with environmental stress. Here, we cloned the Cu/ZnSOD (LdCZS) and MnSOD (LdMS) mRNA of Lymantria dispar by rapid amplification of cDNA ends (RACE). Afterwards their expression patterns were detected by quantitative real-time polymerase chain reaction (qPCR) after bioinformatic analysis. Read More

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Plant allelochemicals affect tolerance of polyphagous lepidopteran pest Helicoverpa armigera (Hübner) against insecticides.

Pestic Biochem Physiol 2019 Feb 18;154:32-38. Epub 2018 Dec 18.

Key Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops, College of Crop Science, Fujian Agriculture and Forestry University, Jinshan, Fuzhou 350002, China. Electronic address:

Cotton bollworm, Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae), is a polyphagous lepidopteran pest distributed worldwide with a broad spectrum of host plants. However, the mechanism of H. armigera adaptation to various insecticides and defensive allelochemicals in its host plants is not fully understood. Read More

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February 2019

Effect of environmental variables and kdr resistance genotype on survival probability and infection rates in Anopheles gambiae (s.s.).

Parasit Vectors 2018 Oct 26;11(1):560. Epub 2018 Oct 26.

Department of Disease Control, London School of Hygiene & Tropical Medicine, London, UK.

Background: Environmental factors, especially ambient temperature and relative humidity affect both mosquitoes and malaria parasites. The early part of sporogony is most sensitive and is affected by high temperatures and temperature fluctuation immediately following ingestion of an infectious blood meal. The aim of this study was to explore whether environmental variables such as temperature, together with the presence of the kdr insecticide resistance mutations, have an impact on survival probability and infection rates in wild Anopheles gambiae (s. Read More

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October 2018

Influence of midgut microbiota in Anopheles stephensi on Plasmodium berghei infections.

Malar J 2018 Oct 25;17(1):385. Epub 2018 Oct 25.

ICMR-National Institute of Malaria Research, Sector 8, Dwarka, New Delhi, 110077, India.

Background: The native gut microbiota of Anopheles mosquitoes is known to play a key role in the physiological function of its host. Interestingly, this microbiota can also influence the development of Plasmodium in its host mosquitoes. In recent years, much interest has been shown in the employment of gut symbionts derived from vectors in the control of vector-borne disease transmission. Read More

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October 2018

Gut-Associated Bacteria of Helicoverpa zea Indirectly Trigger Plant Defenses in Maize.

J Chem Ecol 2018 Aug 22;44(7-8):690-699. Epub 2018 May 22.

Department of Entomology, Pennsylvania State University, University Park, PA, 16802, USA.

Insect-associated microbes can contribute to the physiological and ecological functions of insects. Despite a few examples in beetles and piercing-sucking insects, the varied mechanisms of how insect-associated bacteria mediate plant-insect interactions are still not fully understood. The polyphagous herbivore Helicoverpa zea is a major agricultural pest that harbors certain microbes in their digestive systems. Read More

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Nuclear factor erythroid-derived 2-related factor 2 activates glutathione S-transferase expression in the midgut of Spodoptera litura (Lepidoptera: Noctuidae) in response to phytochemicals and insecticides.

Insect Mol Biol 2018 08 10;27(4):522-532. Epub 2018 May 10.

Guangzhou Key Laboratory of Insect Development Regulation and Applied Research, Institute of Insect Science and Technology, School of Life Sciences, South China Normal University, Guangzhou, China.

Detoxication enzymes play an important role in insect resistance to xenobiotics such as insecticides and phytochemicals. We studied the pathway for activating the expression of glutathione S-transferases (GSTs) in response to selected xenobiotics. An assay of the promoter activity of GST epsilon 1 (Slgste1) of Spodoptera litura led to the discovery of a cis-regulating element. Read More

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Identification and Functional Analysis of a Novel Cytochrome P450 Gene CYP9A105 Associated with Pyrethroid Detoxification in Spodoptera exigua Hübner.

Int J Mol Sci 2018 Mar 5;19(3). Epub 2018 Mar 5.

Guangdong Engineering Research Center for Modern Eco-Agruculture and Circular Agriculture, Guangzhou 510642, China.

In insects, cytochrome P450 monooxygenases (P450s or CYPs) are known to be involved in the detoxification and metabolism of insecticides, leading to increased resistance in insect populations. is a serious polyphagous insect pest worldwide and has developed resistance to various insecticides. In this study, a novel CYP3 clan P450 gene was identified and characterized from . Read More

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Insight into the Mode of Action of Celangulin V on the Transmembrane Potential of Midgut Cells in Lepidopteran Larvae.

Toxins (Basel) 2017 12 6;9(12). Epub 2017 Dec 6.

College of Plant Protection, Northwest A&F University, Yangling 712100, China.

Celangulin V (CV) is the main insecticidal constituent of . The V-ATPase H subunit of the midgut cells of lepidopteran larvae is the putative target protein of CV. Here, we compared the effects of CV on the midgut membrane potentials of and larvae with those of the Cry1Ab toxin from and with those of inactive CV-MIA, a synthetic derivative of CV. Read More

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December 2017

Identification of a novel toll gene (Shtoll3) from the freshwater crab Sinopotamon henanense and its expression pattern changes in response to cadmium followed by Aeromonas hydrophila infection.

Fish Shellfish Immunol 2017 Dec 7;71:177-190. Epub 2017 Oct 7.

School of Life Science, Shanxi University, 92 Wucheng Road, Taiyuan 030006, China. Electronic address:

Toll signaling is essential for expression of immune genes which are important for defense against bacterial, fungal and viral infections in invertebrates. Although several toll genes have been identified in the crustaceans, none of them has been investigated in freshwater crab Sinopotamon henanense. Moreover, the effect of cadmium (Cd) on toll gene expression has never been examined on the freshwater crabs which live in the sediments and are prone to heavy metal bioaccumulation. Read More

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December 2017

SWATH-based quantitative proteomics reveals the mechanism of enhanced Bombyx mori nucleopolyhedrovirus-resistance in silkworm reared on UV-B treated mulberry leaves.

Proteomics 2017 Jul 29;17(13-14). Epub 2017 Jun 29.

College of Biomedical Engineering & Instrument Science, Zhejiang University, Hangzhou, P. R. China.

Bombyx mori nucleopolyhedrovirus (BmNPV) is one of the most acute infectious diseases in silkworm, which has led to great economic loss in sericulture. Previous study showed that the content of secondary metabolites in mulberry leaves, particularly for moracin N, was increased after UV-B irradiation. In this study, the BmNPV resistance of silkworms reared on UV-B treated and moracin N spread mulberry leaves was improved. Read More

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In Vitro Inhibition of Leishmania Attachment to Sandfly Midguts and LL-5 Cells by Divalent Metal Chelators, Anti-gp63 and Phosphoglycans.

Protist 2017 07 29;168(3):326-334. Epub 2017 Mar 29.

Laboratório de Estudos Integrados em Protozoologia, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz (FIOCRUZ), Rio de Janeiro, Brazil.

Leishmania braziliensis and Leishmania infantum are the causative agents of cutaneous and visceral leishmaniasis, respectively. Several aspects of the vector-parasite interaction involving gp63 and phosphoglycans have been individually assayed in different studies. However, their role under the same experimental conditions was not studied yet. Read More

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Termiticidal lectins from Myracrodruon urundeuva (Anacardiaceae) cause midgut damage when ingested by Nasutitermes corniger (Isoptera: Termitidae) workers.

Pest Manag Sci 2017 May 13;73(5):991-998. Epub 2016 Sep 13.

Departamento de Bioquímica, Centro de Biociências, Universidade Federal de Pernambuco, Cidade Universitária, Recife, Pernambuco, Brazil.

Background: Myracrodruon urundeuva is a hardwood tree, and its bark, heartwood and leaf contain lectins (MuBL, MuHL and MuLL respectively) with termiticidal activity against Nasutitermes corniger. In this work, the effects of these lectins on the midgut of N. corniger workers were evaluated. Read More

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Apoptosis-related genes control autophagy and influence DENV-2 infection in the mosquito vector, Aedes aegypti.

Insect Biochem Mol Biol 2016 09 12;76:70-83. Epub 2016 Jul 12.

Eck Institute for Global Health and Department of Biological Sciences, University of Notre Dame, Notre Dame, IN 46556, USA. Electronic address:

The mosquito Aedes aegypti is the primary urban vector for dengue virus (DENV) worldwide. Insight into interactions occurring between host and pathogen is important in understanding what factors contribute to vector competence. However, many of the molecular mechanisms for vector competence remain unknown. Read More

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September 2016


Arch Insect Biochem Physiol 2016 Jul 14;92(3):173-91. Epub 2016 Mar 14.

College of Agronomy, Northeast Agricultural University, Harbin, China.

Two cDNA sequences encoding a trypsin-like and a chymotrypsin-like serine protease (MsT and MsCT, GenBank accession Nos. KP730443 and KP730444, respectively) were cloned from midgut of oriental armyworm, Mythimna separata Walker. Multiple alignments revealed that the deduced amino acid sequences of MsT and MsCT contained a serine protease catalytic motif GDSGGPL and catalytic triads (His, Asp, and Ser). Read More

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Exposure to deltamethrin affects development of Plasmodium falciparum inside wild pyrethroid resistant Anopheles gambiae s.s. mosquitoes in Uganda.

Parasit Vectors 2016 Feb 24;9:100. Epub 2016 Feb 24.

Malaria Consortium, London, UK.

Background: Pyrethroid resistance in African vector mosquitoes is a threat to malaria control. Resistant mosquitoes can survive insecticide doses that would normally be lethal. We studied effects of such doses on Plasmodium falciparum development inside kdr-resistant Anopheles gambiae s. Read More

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February 2016

Technical data of the transcriptomic analysis performed on tsetse fly symbionts, Sodalis glossinidius and Wigglesworthia glossinidia, harbored, respectively by non-infected, Trypanosoma brucei gambiense infected and self-cured Glossina palpalis gambiensis tsetse flies.

Genom Data 2015 Jun 14;4:133-6. Epub 2015 Apr 14.

UMR1090 TAGC, INSERM, Marseille F-13288, France ; Aix-Marseille University, Marseille F-13288, France.

Microarray is a powerful and cheap method to identify and quantify gene expression in particular in a mix of total RNA extracted from biological samples such as the tsetse fly gut, including several organisms (here, the fly tissue and the intestinal microorganisms). Besides, biostatistics and bioinformatics allow comparing the transcriptomes from samples collected from differently treated flies, and thus to identify and quantify differential expressed genes. Here, we describe in details a whole microarray transcriptome dataset produced from tsetse flies symbionts, Sodalis glossinidius and Wigglesworthia glossinidia. Read More

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Detection of viable plasmodium ookinetes in the midguts of anopheles coluzzi using PMA-qrtPCR.

Parasit Vectors 2015 Sep 15;8:455. Epub 2015 Sep 15.

Department of Life Sciences, Imperial College London, London, UK.

Background: Mosquito infection with malaria parasites depends on complex interactions between the mosquito immune response, the parasite developmental program and the midgut microbiota. Simultaneous monitoring of the parasite and bacterial dynamics is important when studying these interactions. PCR based methods of genomic DNA (gDNA) have been widely used, but their inability to discriminate between live and dead cells compromises their application. Read More

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September 2015

Effects of fluoranthene on the fitness-related traits and antioxidative defense in Lymantria dispar L.

Environ Sci Pollut Res Int 2015 Jul 16;22(13):10367-74. Epub 2015 May 16.

Department of Insect Physiology and Biochemistry, Institute for Biological Research "Siniša Stanković", University of Belgrade, Despot Stefan Blvd. 142, Belgrade, Serbia,

This study aimed to examine the effects of ubiquitous polycyclic aromatic hydrocarbon fluoranthene, supplemented to an artificial diet, on the fitness-related traits and activity of midgut antioxidative enzymes-superoxide dismutase (SOD) and catalase (CAT), and expression of their isoforms in the fifth-instar gypsy moth Lymantria dispar L. Prolonged duration of development and reduced weight and relative growth rate were recorded in larvae reared on the diets supplemented with different concentrations of fluoranthene. SOD and CAT activities were significantly higher in the midguts of fluoranthene-treated larvae, compared to that of the control group. Read More

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Imidacloprid impairs the post-embryonic development of the midgut in the yellow fever mosquito Stegomyia aegypti (=Aedes aegypti).

Med Vet Entomol 2015 Sep 13;29(3):245-54. Epub 2015 May 13.

Departamento de Biologia Geral, Universidade Federal de Viçosa, Viçosa, Minas Gerais, Brazil.

The mosquito Stegomyia aegypti (=Aedes aegypti) (Diptera: Culicidae) is a vector for the dengue and yellow fever viruses. As blood digestion occurs in the midgut, this organ constitutes the route of entry of many pathogens. The effects of the insecticide imidacloprid on the survival of St. Read More

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September 2015

Chitin is a component of the Rhodnius prolixus midgut.

Insect Biochem Mol Biol 2016 Feb 22;69:61-70. Epub 2015 Apr 22.

Instituto de Química, Universidade Federal do Rio de Janeiro, RJ, 21941-909, Brazil; Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular, Rio de Janeiro, Brazil. Electronic address:

Chitin is an essential component of the peritrophic matrix (PM), which is a structure that lines the insect's gut and protects against mechanical damage and pathogens. Rhodnius prolixus (Hemiptera: Reduviidae) does not have a PM, but it has an analogous structure, the perimicrovillar membrane (PMM); chitin has not been described in this structure. Here, we show that chitin is present in the R. Read More

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February 2016

Innexin AGAP001476 is critical for mediating anti-Plasmodium responses in Anopheles mosquitoes.

J Biol Chem 2014 Sep 17;289(36):24885-97. Epub 2014 Jul 17.

From the Section of Infectious Diseases, Department of Internal Medicine, Yale University School of Medicine, New Haven, Connecticut 06520 and the Howard Hughes Medical Institute, Chevy Chase, Maryland 20815.

The Toll and IMD pathways are known to be induced upon Plasmodium berghei and Plasmodium falciparum infection, respectively. It is unclear how Plasmodium or other pathogens in the blood meal and their invasion of the midgut epithelium would trigger the innate immune responses in immune cells, in particular hemocytes. Gap junctions, which can mediate both cell-to-cell and cell-to-extracellular communication, may participate in this signal transduction. Read More

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September 2014

Effect of midgut proteolytic activity on susceptibility of lepidopteran larvae to Bacillus thuringiensis subsp. Kurstaki.

Front Physiol 2013 16;4:406. Epub 2014 Jan 16.

Department of Plant Protection, College of Agriculture and Natural Resources, University of Tehran Karaj, Iran.

Bacillus thuringiensis (Bt) is the most effective microbial control agent for controlling numerous species from different insect orders. All subspecies and strains of B. thuringiensis can produce a spore and a crystalline parasporal body. Read More

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January 2014

Effects of midgut bacteria and two protease inhibitors on the reproductive potential and midgut enzymes of Culex pipiens infected with Wuchereria bancrofti.

J Egypt Soc Parasitol 2013 Aug;43(2):537-46

Department of Zoology, Faculty of Science, Al-Azhar University, Nasr City, Cairo, Egypt.

Laboratory investigations were carried out to asses the effect of some proteases inhibitors on the reproductive potential of Culex pipiens females resulted from larvae treated with different protease inhibitors. The fecundity and engorgement of symbiotic and aposymbiotic C. pipiens females were significantly reduced. Read More

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Camptothecin-induced expression of programmed cell death gene 11 in Spodoptera litura.

Pest Manag Sci 2014 Apr 9;70(4):603-9. Epub 2013 Jul 9.

Key Laboratory of Pesticide and Chemical Biology, College of Natural Resources and Environment, South China Agricultural University, Guangzhou, Guangdong Province, China.

Background: Camptothecin, one of the main active components of extract from the bark of the Chinese camptotheca tree, has been reported as a potent insecticide against various insect species. However, the mechanism of action of camptothecin as a botanical pesticide is not completely understood.

Results: In this study, the full-length cDNA (GeneBank number JF681972) of Spodoptera litura programmed cell death protein 11 (pcdp 11) isoform 1 was cloned, sequenced and characterised. Read More

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