4,039 results match your criteria microsporidia

Molecular detection and genotyping of Enterocytozoon bieneusi in pet dogs in Xinjiang, Northwestern China.

Parasite 2021 20;28:57. Epub 2021 Jul 20.

College of Animal Science, Tarim University, Alar, Xinjiang 843300, PR China.

Enterocytozoon bieneusi is an obligate intracellular parasitic fungi that infects a wide range of mammalian hosts. However, the literature is lacking information regarding the presence and diversity of E. bieneusi genotypes in domesticated dogs in Northwestern China. Read More

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Microsporidial Endotheliitis Mimicking Graft Rejection After Deep Anterior Lamellar Keratoplasty.

Cornea 2021 Jul 15. Epub 2021 Jul 15.

Cornea & Anterior Segment Service, L V Prasad Eye Institute, Bhubaneswar, Odisha, India; Kanupriya Dalmia Ophthalmic Pathology Laboratory, L V Prasad Eye Institute, Bhubaneswar, Odisha, India; and Ocular Microbiology Service, L V Prasad Eye Institute, MTC Campus, Bhubaneswar, Odisha, India.

Purpose: The purpose of this study was to report a case of microsporidial endotheliitis masquerading as graft rejection after deep anterior lamellar keratoplasty (DALK).

Methods: A 36-year-old man visited the clinic with complaints of blurred vision, redness, pain, watering, and whitish appearance of the black portion of his left eye. On evaluation, there was diffuse stromal edema with epithelial defect and hypopyon. Read More

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Microsporidia Have a Peculiar Outer Membrane with Exterior Cytoplasmic Proteins.

Acta Parasitol 2021 Jul 15. Epub 2021 Jul 15.

Gulf Coast Research Laboratory, University of Southern Mississippi, Ocean Springs, Mississippi, 39564, USA.

Purpose: Most eukaryotic cells have a plasma membrane with glycoproteins on the outer leaflet and cytoplasmic proteins on the inner leaflet. However, the microsporidians examined have a peculiar outer membrane with cytoplasmic proteins on the surface. His study was to determine if this is true and identify the presence of key cytoplasmic proteins on the exterior of the sporoplasm and spore stages. Read More

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Corneal Involvement in HIV-infected Individuals.

Ocul Immunol Inflamm 2021 Jul 7:1-6. Epub 2021 Jul 7.

Cornea and Refractive Surgery, Aravind Eye Care System, Madurai, India.

Corneal involvement in HIV-infected individuals may be broadly classified into two categories, namely, infectious and noninfectious with the vast majority of manifestations occurring in the former. In this article, we shall focus on these two categories and strive to highlight those presentations that should alert the clinician to suspect underlying HIV infection. Infectious group mainly consists of Herpitic group of viral infections. Read More

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Characterizing the Xenoma of Provides Insights Into the Most Efficient Mode of Microsporidian Proliferation.

Front Cell Infect Microbiol 2021 16;11:699239. Epub 2021 Jun 16.

State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, China.

Microsporidia are a group of obligated intracellular parasites that can infect nearly all vertebrates and invertebrates, including humans and economic animals. Microsporidian is a natural pathogen of multiple insects and can massively proliferate by making tumor-like xenoma in host tissue. However, little is known about the subcellular structures of this xenoma and the proliferation features of the pathogens inside. Read More

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Gut Microbiota Dynamics in Natural Populations of under Experimental Infection with .

Microorganisms 2021 Jun 4;9(6). Epub 2021 Jun 4.

Grupo de Microbiodiversidad y Bioprospección, Laboratorio de Biología Celular y Molecular, Universidad Nacional de Colombia sede Medellín, Street 59A #63-20, Medellín 050003, Colombia.

is recognized by its vectorial competence in the transmission of parasites that cause fatal visceral leishmaniasis in rural and urban environments of the Caribbean coast of Colombia. The effect on and the variation of the gut microbiota in female infected with were evaluated under experimental conditions using 16S rRNA Illumina MiSeq sequencing. In the coinfection assay with , 96. Read More

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Microsporidiosis in Humans.

Clin Microbiol Rev 2021 Jun 30:e0001020. Epub 2021 Jun 30.

Department of Pathology, Albert Einstein College of Medicine, New York, New York, USA.

Microsporidia are obligate intracellular pathogens identified ∼150 years ago as the cause of pébrine, an economically important infection in silkworms. There are about 220 genera and 1,700 species of microsporidia, which are classified based on their ultrastructural features, developmental cycle, host-parasite relationship, and molecular analysis. Phylogenetic analysis suggests that microsporidia are related to the fungi, being grouped with the Cryptomycota as a basal branch or sister group to the fungi. Read More

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Generation of a Microsporidia Species Attribute Database and Analysis of the Extensive Ecological and Phenotypic Diversity of Microsporidia.

mBio 2021 06 29;12(3):e0149021. Epub 2021 Jun 29.

Department of Molecular Genetics, University of Toronto, Toronto, Ontario, Canada.

Microsporidia are a large group of fungus-related obligate intracellular parasites. Though many microsporidia species have been identified over the past 160 years, depiction of the full diversity of this phylum is lacking. To systematically describe the characteristics of these parasites, we created a database of 1,440 species and their attributes, including the hosts they infect and spore characteristics. Read More

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A Systematic Review and Meta-analysis on the Global Molecular Epidemiology of Microsporidia Infection Among Rodents: A Serious Threat to Public Health.

Acta Parasitol 2021 Jun 27. Epub 2021 Jun 27.

Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.

Background: Microsporidiosis as a zoonotic disease has caused serious health problems in high-risk groups, including immunosuppressed individuals. Among the potential animal reservoirs of microsporidia, rodents play a key role due to close-contact with humans and their dispersion in different environments. Therefore, this systematic review and meta-analysis aimed to assess the global status and genetic diversity of microsporidia infection in different rodents. Read More

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Detection of , , spp. and microsporidia in fresh produce using molecular methods: - A review.

Food Waterborne Parasitol 2021 Jun 26;23:e00124. Epub 2021 Apr 26.

Veterinary Research Institute, Hudcova 296/70, Brno 621 00, Czech Republic.

The current trend for a healthy lifestyle corresponds with a healthy diet, which is associated with regular and frequent consumption of raw fruit and vegetables. However, consumption of ready-to-eat (RTE) food without heat treatment or sufficient washing may pose a risk to consumers. Among the well-known protozoan parasites associated with RTE food and water are spp. Read More

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Microsporidial Keratitis Related to Water Exposure: A Case Series.

Cureus 2021 Jun 19;13(6):e15760. Epub 2021 Jun 19.

Ophthalmology, Eye Physicians and Surgeons of Augusta, PC, Augusta, USA.

The objective of this retrospective study was to present a series of cases involving the rare ocular disease of microsporidia keratitis treated at a private practice clinic and describe the details regarding specific water exposure, clinical course, voriconazole treatment, and increased prevalence of this infection in Augusta, Georgia, USA. Our analysis was based on the accumulated data from all patients (n=15) diagnosed with microsporidia keratitis at our private practice clinic; the clinical course of three cases is discussed in detail in this article. Specific environmental exposures were documented in 10 patients. Read More

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Parasites, pathogens, and other symbionts of copepods.

Trends Parasitol 2021 Jun 19. Epub 2021 Jun 19.

Department of Life Sciences, The Natural History Museum, Cromwell Road, London SW7 5BD, UK.

There is a large diversity of eukaryotic symbionts of copepods, dominated by epizootic protists such as ciliates, and metazoan parasites. Eukaryotic endoparasites, copepod-associated bacteria, and viruses are less well known, partly due to technical limitations. However, new molecular techniques, combined with a range of other approaches, provide a complementary toolkit for understanding the complete symbiome of copepods and how the symbiome relates to their ecological roles, relationships with other biota, and responses to environmental change. Read More

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Genome and Evolutionary Analysis of : A Microsporidian Parasite of Honey Bees.

Front Microbiol 2021 2;12:645353. Epub 2021 Jun 2.

US Department of Agriculture-Aricultural Research Service (USDA-ARS) Bee Research Laboratory, Beltsville, MD, United States.

Microsporidia comprise a phylum of single cell, intracellular parasites and represent the earliest diverging branch in the fungal kingdom. The microsporidian parasite primarily infects honey bee gut epithelial cells, leading to impaired memory, suppressed host immune responses and colony collapse under certain circumstances. As the genome of is challenging to assembly due to very high genetic diversity and repetitive region, the genome was re-sequenced using long reads. Read More

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Molecular epidemiology of Microsporidia among HIV-positive and HIV-negative patients in the Limpopo province, South Africa.

J Infect Dev Ctries 2021 May 31;15(5):710-718. Epub 2021 May 31.

Department of Medical Laboratory Sciences, College of Health Sciences, University of Sharjah, Sharjah, United Arab Emirates.

Introduction: Human microsporidiosis represents an important and rapidly emerging opportunistic disease. The present study investigated the prevalence of microsporidia among HIV positive and HIV negative patients with or without diarrhoea in Vhembe and Mopani Districts in the Limpopo Province.

Methodology: A total of 170 stool samples were collected from these patients and microsporidia species was detected using a Real-Time PCR targeting a conserved region of the small ribosomal subunit rRNA (SSU-rRNA) gene of Enterocytozoon bieneusi, Encephalitozoon intestinalis, Encephalitozoon hellem, and Encephalitozoon cuniculi. Read More

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Von Willebrand Factor Facilitates Intravascular Dissemination of Microsporidia .

Front Cell Infect Microbiol 2021 21;11:694957. Epub 2021 May 21.

State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, China.

Microsporidia are a group of spore-forming, fungus-related pathogens that can infect both invertebrates and vertebrates including humans. The primary infection site is usually digestive tract, but systemic infections occur as well and cause damages to organs such as lung, brain, and liver. The systemic spread of microsporidia may be intravascular, requiring attachment and colonization in the presence of shear stress. Read More

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A phylogenetic framework to investigate the microsporidian communities through metabarcoding and its application to lake ecosystems.

Environ Microbiol 2021 Jun 3. Epub 2021 Jun 3.

CNRS, Laboratoire Microorganismes: Génome et Environnement, Université Clermont Auvergne, Clermont-Ferrand, F-63000, France.

Microsporidia are obligate intracellular eukaryotic parasites known to parasitize many species of the animal kingdom as well as some protists. However, their diversity is underestimated, in part as a consequence of the failure of 'universal' primers to detect them in metabarcoding studies. Besides, due to the inconsistency between taxonomy and phylogenetic data, available databases may assign incorrectly sequences obtained with high-throughput sequencing. Read More

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Comparative Assessment of In-House Real-Time PCRs Targeting Enteric Disease-Associated Microsporidia in Human Stool Samples.

Pathogens 2021 May 26;10(6). Epub 2021 May 26.

Department of Hospital Hygiene & Infectious Diseases, University Medicine Göttingen, 37075 Göttingen, Germany.

Microsporidiosis is an infection predominantly occurring in immunosuppressed patients and infrequently also in travelers. This study was performed to comparatively evaluate the diagnostic accuracy of real-time PCR assays targeting microsporidia with etiological relevance in the stool of human patients in a latent class analysis-based test comparison without a reference standard with perfect accuracy. Thereby, two one-tube real-time PCR assays and two two-tube real-time PCR assays targeting and spp. Read More

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Metatranscriptomic Analysis Reveals an Imbalance of Hepatopancreatic Flora of Chinese Mitten Crab with Hepatopancreatic Necrosis Disease.

Biology (Basel) 2021 May 23;10(6). Epub 2021 May 23.

School of Biology and Basic Medical Science, Soochow University, Suzhou 215123, China.

Hepatopancreas necrosis disease (HPND) of the Chinese mitten crab Eriocheir sinensis causes huge economic loss in China. However, the pathogenic factors and pathogenesis are still a matter of dissension. To search for potential pathogens, the hepatopancreatic flora of diseased crabs with mild symptoms, diseased crabs with severe symptoms, and crabs without visible symptoms were investigated using metatranscriptomics sequencing. Read More

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Susceptibility of the Gypsy Moth (Lepidoptera: Erebidae) to (Microsporidia: Nosematidae).

Insects 2021 May 14;12(5). Epub 2021 May 14.

All-Russian Institute of Plant Protection, Podbelskogo 3, Pushkin, 196608 St. Petersburg, Russia.

The gypsy moth, , is a notorious forest defoliator, and various pathogens are known to act as natural regulators of its population density. As a widespread herbivore with a broad range of inhabited areas and host plants, it is potentially exposed to parasitic microorganisms from other insect hosts. In the present paper, we determined the susceptibility of gypsy moth larvae to the microsporidium from the European corn borer, . Read More

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Proteomic Identification of Organelles Using the Engineered Ascorbate Peroxidase APEX and Development of Silkworm Organelle Proteome Database (SilkOrganPDB).

Int J Mol Sci 2021 May 10;22(9). Epub 2021 May 10.

State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400715, China.

Silkworm is an economically important insect and a lepidopteran model. Organelle proteome is vital to understanding gene functions; however, it remains to be identified in silkworm. Here, using the engineered ascorbate peroxidase APEX, we constructed transgenic embryo cells (BmE) expressing APEX-NLS, COX4-APEX, APEX-Rev, and APEX-KDEL in nucleus, mitochondrial matrix (MM), cytosol, and endoplasmic reticulum (ER), and isolated the biotin-labeled proteins using streptavidin-affinity purification, respectively. Read More

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Molecular and microscopic prevalence of intestinal microsporidia among HIV+/AIDS patients in the Alborz province, Iran.

Trans R Soc Trop Med Hyg 2021 Jun 1. Epub 2021 Jun 1.

Department of Parasitology and Mycology, School of Medicine, Alborz University of Medical Sciences, Karaj, Alborz Province, Iran, 3149779453.

Background: Microsporidia are a large family of obligate intracellular protozoa; these medically important species are recognized as opportunistic agents in intestinal complications in HIV+/AIDS patients.

Methods: The current cross-sectional study was designed and conducted from October 2018 to June 2019 to determine intestinal microsporidia in HIV+/AIDS patients by trichrome/Zeihl-Neelsen staining and SYBR Green-based real-time PCR.

Results: Out of 80 HIV+/AIDS patients, 23. Read More

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Identification of pathogens in the invasive hornet Vespa velutina and in native Hymenoptera (Apidae, Vespidae) from SW-Europe.

Sci Rep 2021 May 27;11(1):11233. Epub 2021 May 27.

CiMUS P2D2, Universidade de Santiago de Compostela, Av. de Barcelona s/n, 15782, Santiago de Compostela, Galiza, Spain.

Invasive species contribute to deteriorate the health of ecosystems due to their direct effects on native fauna and the local parasite-host dynamics. We studied the potential impact of the invasive hornet Vespa velutina on the European parasite-host system by comparing the patterns of diversity and abundance of pathogens (i.e. Read More

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Muscular microsporidian infection in Arctic char Salvelinus alpinus from two lakes in Nunavik, Quebec, Canada.

Dis Aquat Organ 2021 May 27;144:209-220. Epub 2021 May 27.

Centre québécois sur la santé des animaux sauvages / Canadian Wildlife Health Cooperative, Faculté de médecine vétérinaire, Université de Montréal, St. Hyacinthe, Quebec J2S 2M2, Canada.

Arctic char Salvelinus alpinus is an important cultural and subsistence resource for Inuit communities. Muscular infections by microsporidia were diagnosed for the first time in Arctic char originating from 2 different lakes in Nunavik (Quebec, Canada). The consumption of these infected fish was associated with digestive tract disorders in people. Read More

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First record of a new microsporidium pathogenic to Gonipterus platensis in Brazil.

Sci Rep 2021 May 26;11(1):10971. Epub 2021 May 26.

School of Agricultural Sciences, São Paulo State University (UNESP), Campus of Botucatu, Av. Universitária, 3780, Altos do Paraíso, Fazenda Experimental Lageado, Botucatu, SP, 18610-034, Brazil.

Microsporidia are naturally occurring fungal-related parasites that can infect nearly all animal hosts, but their biocontrol potential of insect pests is routinely overlooked in agriculture and forestry. This research brings the first report describing the natural occurrence of a microsporidium causing disease in field-collected populations of the invasive eucalyptus snout beetle, Gonipterus platensis (Coleoptera: Curculionidae), a major destructive pest of eucalyptus plantations in Brazil. Adult beetles were collected during field surveys in commercial eucalyptus plantations in southern Brazil to be examined and dissected with typical symptoms to verify presence of microsporidian spores in haemolymph. Read More

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Agri-environment scheme nectar chemistry can suppress the social epidemiology of parasites in an important pollinator.

Proc Biol Sci 2021 05 26;288(1951):20210363. Epub 2021 May 26.

Centre for Ecology, Evolution and Behaviour, Department of Biological Sciences, School of Life Sciences and the Environment, Royal Holloway University of London, Egham, UK.

Emergent infectious diseases are one of the main drivers of species loss. Emergent infection with the microsporidian has been implicated in the population and range declines of a suite of North American bumblebees, a group of important pollinators. Previous work has shown that phytochemicals found in pollen and nectar can negatively impact parasites in individuals, but how this relates to social epidemiology and by extension whether plants can be effectively used as pollinator disease management strategies remains unexplored. Read More

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Two parasites in one host: spatiotemporal dynamics and co-occurrence of Microsporidia and in an amphipod host.

Parasitology 2021 Aug 24;148(9):1099-1106. Epub 2021 May 24.

Department of Zoology, University of Otago, 340 Great King Street, Dunedin 9016, New Zealand.

Biological interactions can greatly influence the abundance of species. This is also true for parasitic species that share the same host. Microsporidia and Rickettsia are widespread intracellular parasites in populations of Paracalliope fluviatilis, the most common freshwater amphipods in New Zealand. Read More

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Identification of novel miRNAs from the microsporidian parasite Nosema ceranae.

Infect Genet Evol 2021 May 20;93:104930. Epub 2021 May 20.

Jiangxi Key laboratory of Honeybee Biology and Beekeeping, Jiangxi Agricultural University, Zhimin Ave. 1101, Nanchang 330045, China; Honeybee Research Institute, Jiangxi Agricultural University, Zhimin Ave. 1101, Nanchang 330045, China. Electronic address:

Previously, six miRNAs were identified from the microsporidian parasite Nosema ceranae. By taking advantage of the recently updated N. ceranae and honey bee genome assemblies, we re-analyzed the deep sequencing datasets. Read More

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Sequelae of microsporidial keratoconjunctivitis and its management.

Indian J Ophthalmol 2021 06;69(6):1537-1543

Cornea and Anterior Segment Services, Mithu Tulsi Chanrai Campus, L.V. Prasad Eye Institute, Bhubaneswar, Odisha, India.

Purpose: : To characterize the sequelae of microsporidia keratoconjunctivitis (MKC) and outline its management.

Methods: Retrospective analysis of microbiologically proven MKC returned with persistent disease between January 2015 and December 2019 was done. Demographics, clinical features, management, and outcome were analyzed. Read More

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"A prospective study on the clinical course and proposed morphological classification scheme of microsporidial keratoconjunctivitis."

Semin Ophthalmol 2021 May 19:1-6. Epub 2021 May 19.

Cornea and Anterior Segment Services, L. V. Prasad Eye Institute, Odisha, India.

: To describe the clinical course and propose a morphological classification scheme of microsporidial keratoconjunctivitis. : This is a prospective study at L V Prasad Eye Institute, Odisha, between August-January (2017-19). Patients of any age or sex, microbiologically proven as microsporidial keratoconjunctivitis were included. Read More

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Raw Goat's Milk, Fresh and Soft Cheeses as a Potential Source of .

Foodborne Pathog Dis 2021 May 11. Epub 2021 May 11.

Institute of Parasitology, Biology Centre of the Czech Academy of Sciences, České Budějovice, Czech Republic.

The aim of this work was to evaluate the effect of pasteurization and coagulation during goat cheese production on the infectivity of spores for immunodeficient (SCID, CD4, and CD8) and immunocompetent (BALB/c and C57BL/6) mice. Goat milk and fecal samples were screened for the presence and quantity of microsporidial DNA using molecular methods. Experimentally produced cheese from -enriched goat milk or goat cheese purchased from retail producers was fed with experimental mice susceptible to infection. Read More

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