3,824 results match your criteria microbiology implant

Zwitterionic polydopamine coatings suppress silicone implant-induced capsule formation.

Biomater Sci 2021 May;9(9):3425-3432

Department of Plastic and Reconstructive Surgery, Chungnam National University College of Medicine, Daejeon 35015, Republic of Korea.

A synthetic zwitterionic dopamine derivative (ZW-DOPA) containing both catechol and amine groups was recently shown to exhibit excellent antifouling activity on marine surfaces. Here, we have extended these analyses to investigate the effects of ZW-DOPA coating on silicone implants. Successful formation of ZW-DOPA coatings on silicone implants was confirmed based on a combination of decreased static water contact angles on silicone implants, evidence of new peaks at 400. Read More

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A spacer infection by Candida albicans secondary to a Staphylococcus capitis prosthetic joint infection: a case report.

BMC Infect Dis 2021 May 4;21(1):416. Epub 2021 May 4.

IRCCS Istituto Ortopedico Galeazzi, Laboratory of Clinical Chemistry and Microbiology, Via R. Galeazzi 4, 20161, Milan, Italy.

Background: Prosthetic joint infection (PJI) is one of the most feared complications following total arthroplasty surgeries. Gram-positive bacteria are the most common microorganisms implicated in PJIs, while infections mediated by fungi only account for 1% of cases. When dealing with PJIs, a two-stage revision arthroplasty is widely used. Read More

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Systemic antibiotic therapy as an adjunct to non-surgical peri-implantitis treatment: A single-blind RCT.

J Clin Periodontol 2021 May 3. Epub 2021 May 3.

Center for Dentistry and Oral Hygiene, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands.

Aim: The aim of this single-blind RCT was to evaluate the adjunctive clinical and microbiological effect of systemic amoxicillin (AMX) plus metronidazole (MTZ) to non-surgical treatment of peri-implantitis.

Material And Methods: Patients (N = 62) with peri-implantitis were randomly assigned to receive full-mouth mechanical debridement and decontamination and use of chlorhexidine (control group) or combined with antibiotic therapy of AMX/MTZ (test group). Primary outcome was change in bleeding score from baseline (T ) to 3-month follow-up (T ). Read More

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Surface Treatment of the Dental Implant with Hyaluronic Acid: An Overview of Recent Data.

Int J Environ Res Public Health 2021 Apr 27;18(9). Epub 2021 Apr 27.

Department of Biomedical and Dental Sciences, Morphological and Functional Images, University of Messina, 98100 Messina, Italy.

Recently, interest has grown by focusing on the evaluation of a molecule already produced in the human body such as hyaluronic acid (HA), as an application to the surface of the titanium implant. Its osteo-conductive characteristics and positive interaction with the progenitor cells responsible for bone formation, consequently, make it responsible for secondary stability. The aim of this work was to analyze the various surface treatments in titanium implants, demonstrating that the topography and surface chemistry of biomaterials can correlate with the host response; also focusing on the addition of HA to the implant surface and assessing the biological implications during early stages of recovery. Read More

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Biofilm Formation on Dental Implant Biomaterials by Strains Isolated from Patients with Cystic Fibrosis.

Materials (Basel) 2021 Apr 17;14(8). Epub 2021 Apr 17.

Department of Medical Biology, Medical University of Warsaw, Litewska 14/16, 00-575 Warsaw, Poland.

Implants made of ceramic and metallic elements, which are used in dentistry, may either promote or hinder the colonization and adhesion of bacteria to the surface of the biomaterial to varying degrees. The increased interest in the use of dental implants, especially in patients with chronic systemic diseases such as cystic fibrosis (CF), is caused by an increase in disease complications. In this study, we evaluated the differences in the in vitro biofilm formation on the surface of biomaterials commonly used in dentistry (Ti-6Al-4V, cobalt-chromium alloy (CoCr), and zirconia) by isolated from patients with CF. Read More

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Prosthetic Shoulder Joint Infection by : Does Rifampin Improve Prognosis? A Retrospective, Multicenter, Observational Study.

Antibiotics (Basel) 2021 Apr 21;10(5). Epub 2021 Apr 21.

Infectious Diseases Unit, Internal Medicine Department, Hospital Universitari Son Espases, Fundació Institut d'Investigació Sanitària Illes Balears (IdISBa), 07120 Palma de Mallorca, Spain.

This retrospective, multicenter observational study aimed to describe the outcomes of surgical and medical treatment of -related prosthetic joint infection (PJI) and the potential benefit of rifampin-based therapies. Patients with -related PJI who were diagnosed and treated between January 2003 and December 2016 were included. We analyzed 44 patients with -related PJI (median age, 67. Read More

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Antibiotic Prescribing Habits and Antimicrobial Resistance Awareness of Dental Practitioners in Primorsko-Goranska County, Croatia.

Microb Drug Resist 2021 Apr 28. Epub 2021 Apr 28.

Department of Clinical Microbiology, University Hospital for Infectious Diseases, "Dr. Fran Mihaljević," Zagreb, Croatia.

The goal of this study was to investigate the differences in dentists' knowledge, attitudes, and practice regarding antibiotic use and resistance among two areas of Primorsko-Goranska County (P-GC), Croatia. A cross-sectional study based on a structured questionnaire that was given to 230 dental practitioners in outpatient settings of P-GC in 2018. The overall response rate was 68. Read More

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Weight change among women using intramuscular depot medroxyprogesterone acetate, a copper intrauterine device, or a levonorgestrel implant for contraception: Findings from a randomised, multicentre, open-label trial.

EClinicalMedicine 2021 Apr 6;34:100800. Epub 2021 Apr 6.

MatCH Research Unit (MRU), Department of Obstetrics and Gynaecology, Faculty of Health Sciences, University of the Witwatersrand, Durban, South Africa.

Background: There is limited evidence on the impact of the use of progestin-only hormonal contraception (POC) on weight change. We conducted a secondary analysis of prospective weight change among women enrolled in the Evidence for Contraceptive options and HIV Outcomes (ECHO) trial.

Methods: The ECHO trial was conducted at 12 sites in eSwatini, Kenya, South Africa and Zambia between December 2015 and October 2018. Read More

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Risk factors associated with surgical site infections after thoracic or lumbar surgery: a 6-year single centre prospective cohort study.

J Orthop Surg Res 2021 Apr 15;16(1):265. Epub 2021 Apr 15.

Department of Clinical Pharmacology, Na Homolce Hospital, Prague, Czech Republic.

Background: Surgical site infection (SSI) is a risk in every operation. Infections negatively impact patient morbidity and mortality and increase financial demands. The aim of this study was to analyse SSI and its risk factors in patients after thoracic or lumbar spine surgery. Read More

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The stability of implants and microbiological effects following photobiomodulation therapy with one-stage placement: A randomized, controlled, single-blinded, and split-mouth clinical study.

Clin Implant Dent Relat Res 2021 Apr 14. Epub 2021 Apr 14.

Faculty of Dentistry, Department of Prosthetic Restorative Dentistry, Gazi University, Ankara, Turkey.

Background: Studies have reported a positive effect on bone healing and the elimination of microorganisms placed on the titanium implants, while others have not confirmed the positive photobiomodulation therapy (PBMT) effects on bone regeneration and bone structure around the implants.

Purpose: The aim of the present study was to address the following questions: Does PBMT improve implant stability and affect microbiota around dental implants in the early stage of osseointegration?

Materials And Methods: This study was designed as randomized-controlled prospective, split mouth, single-blinded clinical trial. Implants were randomly divided into two groups and implants placed in the test group were treated with Gallium-aluminum-arsenide (GaAlAs) diode laser with PBMT immediately after surgery and for 15 days (n = 47). Read More

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RNA Sequencing and Pathways Analyses of Middle Ear Epithelia From Patients With Otitis Media.

Laryngoscope 2021 Apr 12. Epub 2021 Apr 12.

Department of Otolaryngology and Communication Sciences, Medical College of Wisconsin, Milwaukee, Wisconsin, U.S.A.

Objectives: Otitis media (OM) is the most common pediatric diagnosis in the United States. However, our understanding of the molecular pathogenesis of OM remains relatively poor. Investigation of molecular pathways involved in OM may improve the understanding of this disease process and elucidate novel therapeutic targets. Read More

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Effects of toothbrush abrasion on surface and antibacterial properties of hydroxyapatite-tryptophan complex with gray titania.

Odontology 2021 Apr 9. Epub 2021 Apr 9.

Functional Occlusal Treatment, The Nippon Dental University Graduate School of Life Dentistry At Niigata, 1-8 Hamaura-cho, Chuo-ku, Niigata-shi, Niigata, 951-8580, Japan.

Photocatalysts have attracted attention in the medical field for their antibacterial effects. However, typical photocatalysts are activated by ultraviolet rays, which may have adverse effects. Therefore, we focused on a new photocatalyst that is activated by visible light, hydroxyapatite (HAp), and amino acid complex with gray titania, and evaluated its antibacterial effects against Porphyromonas gingivalis and effect by toothbrushing. Read More

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Microbial community compositions in breast implant biofilms associated with contracted capsules.

PLoS One 2021 8;16(4):e0249261. Epub 2021 Apr 8.

Skinworks, Vancouver, BC, Canada.

Subclinical bacterial infections (biofilms) are strongly implicated in breast augmentation failure due to capsular contracture, and while these infections are generally ascribed to common skin commensals, this remains largely unsubstantiated through robust cultivation independent analyses. To determine capsule biofilm microbial community compositions, we employed amplicon sequencing of the 16S rRNA gene using DNA extracted from breast implant capsule samples. These cultivation independent analyses revealed that capsule associated biofilms are more diverse than canonical single-species infections, but have relatively low diversity (~ <100 species) compared to many host-associated microbial communities. Read More

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Microbiological diagnosis of polymicrobial periprosthetic joint infection revealed superiority of investigated tissue samples compared to sonicate fluid generated from the implant surface.

Int J Infect Dis 2021 Apr 2;106:302-307. Epub 2021 Apr 2.

Agaplesion Bethesda Krankenhaus, Klinik für Unfallchirurgie und Orthopädische Chirurgie, Wuppertal, Germany.

Objectives: In the microbiological diagnosis of periprosthetic joint infection (PJI), there is much discussion about the methodology of obtaining proper specimens, the processing technique, and suitable culture media. This retrospective study was conducted to analyse the accuracy of our culture techniques.

Methods: Tissue samples and components from 258 patients after revision arthroplasty of the hip, knee, and shoulder were investigated, and the results of tissue cultures (TC) were compared to those of sonicate fluid cultures (SFC). Read More

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Novel Peptides Targeting the β-Clamp Rapidly Kill Planktonic and Biofilm Both and .

Front Microbiol 2021 17;12:631557. Epub 2021 Mar 17.

Department of Clinical and Molecular Medicine, Faculty of Medicine and Health Sciences, Norwegian University of Science and Technology (NTNU), Trondheim, Norway.

Antimicrobial resistance is an increasing threat to global health and challenges the way we treat infections. Peptides containing the PCNA interacting motif APIM (APIM-peptides) were recently shown to bind to the bacterial PCNA homolog, the beta (β)-clamp, and to have both antibacterial and anti-mutagenic activities. In this study we explore the antibacterial effects of these peptides on , a bacterial species commonly found in prosthetic joint infections (PJI). Read More

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Associated with Prosthetic Joint Infections: Clinical Features and Genomic Characteristics.

Pathogens 2021 Mar 26;10(4). Epub 2021 Mar 26.

Department of Biomedicine, Aarhus University, 8000 Aarhus, Denmark.

The anaerobic coagulase-negative staphylococcal species is a member of the normal skin microbiota. However, is rarely found in clinical specimens and its pathogenic potential is unclear. The clinical data of prosthetic hip (n = 5) and shoulder (n = 2) joint implant-associated infections where was detected in periprosthetic tissue specimens are described. Read More

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Culture-Independent Genotyping, Virulence and Antimicrobial Resistance Gene Identification of from Orthopaedic Implant-Associated Infections.

Microorganisms 2021 Mar 30;9(4). Epub 2021 Mar 30.

Department of Microbiology and Infection Control, Akershus University Hospital, 1478 Lørenskog, Norway.

Our culture-independent nanopore shotgun metagenomic sequencing protocol on biopsies has the potential for same-day diagnostics of orthopaedic implant-associated infections (OIAI). As OIAI are frequently caused by , we included genotyping and virulence gene detection to exploit the protocol to its fullest. The aim was to evaluate genotyping, virulence and antimicrobial resistance genes detection using the shotgun metagenomic sequencing protocol. Read More

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Osseointegration evaluation of UHMWPE and PEEK-based scaffolds with BMP-2 using model of critical-size cranial defect in mice and push-out test.

J Mech Behav Biomed Mater 2021 Mar 25;119:104477. Epub 2021 Mar 25.

N. F. Gamaleya National Research Center of Epidemiology and Microbiology, Ministry of Health of the Russian Federation, 123098, Gamaleya Str. 18, Moscow, Russia; Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, 119992 Moscow, Russia; All-Russia Research Institute of Agricultural Biotechnology, 127550, Timiryazevskaya Str. 42, Moscow, Russia.

It the present study the push-out mechanical test was adopted for mouse model of implantation in critical-size cranial defects to evaluate the effectiveness of implant-skull fusion. As implants, disks of porous ultra-high molecular weight polyethylene (UHMWPE) and polyetheretherketone (PEEK) with hydroxylapatite (HA) with and without loading of recombinant bone morphogenetic protein-2 (BMP-2) were used. Implantation results were evaluated using histology and micro-computed tomography (micro-CT). Read More

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Correlation Between Biofilm-Formation and the Antibiotic Resistant Phenotype in Isolates: A Laboratory-Based Study in Hungary and a Review of the Literature.

Infect Drug Resist 2021 23;14:1155-1168. Epub 2021 Mar 23.

Department of Pharmacodynamics and Biopharmacy, Faculty of Pharmacy, University of Szeged, Szeged, 6720, Hungary.

Introduction: () is an important causative pathogen in human infections. The production of biofilms by bacteria is an important factor, leading to treatment failures. There has been significant interest in assessing the possible relationship between the multidrug-resistant (MDR) status and the biofilm-producer phenotype in bacteria. Read More

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Protection From COVID-19.

Dtsch Arztebl Int 2021 02;118(5):59-65

Department of Tropical Medicine and Infectious Diseases, Rostock University Medical CenterDepartment of Microbiology & Virology, Brandenburg Medical School Theodor FontaneInstitute for Implant Technology and Biomaterials (IIB); Rostock University Medical Center.

Background: Since the beginning of 2020 the SARS-CoV-2 virus has spread to nearly every country in the world. The mainly airborne pathogen has led to large numbers of deaths, principally in elderly and vulnerable segments of the population. Protective vaccines have recently become available, but it is not yet clear whether and when population-wide immunity will be achieved. Read More

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February 2021

Spatiotemporal patterns of gene expression around implanted silicon electrode arrays.

J Neural Eng 2021 Apr 27;18(4). Epub 2021 Apr 27.

Department of Biomedical Engineering, Michigan State University, East Lansing, MI 48824, United States of America.

Intracortical brain interfaces are an ever evolving technology with growing potential for clinical and research applications. The chronic tissue response to these devices traditionally has been characterized by glial scarring, inflammation, oxidative stress, neuronal loss, and blood-brain barrier disruptions. The full complexity of the tissue response to implanted devices is still under investigation. Read More

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Knee arthrodesis using a custom modular intramedullary nail in failed, infected knee arthroplasties: A concise follow-up note of 31 cases at a median of 13 years post-arthrodesis.

Orthop Traumatol Surg Res 2021 Mar 24:102898. Epub 2021 Mar 24.

Département universitaire de chirurgie orthopédique et traumatologique, University of Lille, CHU de Lille, ULR 4490, 59000 Lille, France; CRIOAC, centre de référence pour le traitement des infections ostéo-articulaires complexes Lille Tourcoing, Lille, France; Service de chirurgie orthopédique, Hôpital Roger-Salengro, CHRU de Lille, rue Émile-Laine, 59037 Lille, France.

Introduction: Knee arthrodesis utilizes an arthrodesis nail as a salvage technique for infected total knee arthroplasty (TKA), especially when the extensor mechanism is damaged, or the skin is compromised. This implant helps to minimize or prevent leg length discrepancy, while allowing immediate weight bearing without requiring bone fusion. However, there is a risk of infection. Read More

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Genome Sequence of Staphylococcus epidermidis AUH4567, a Clinical Isolate from an Infected Central Venous Catheter.

Microbiol Resour Announc 2021 Mar 25;10(12). Epub 2021 Mar 25.

Interdisciplinary Nanoscience Center, Aarhus University, Aarhus, Denmark.

is a common cause of implant-associated infections, and this is related to its ability to form biofilms. Strain-to-strain variability in biofilm formation is likely caused by genetic differences. Here, we present a draft genome of AUH4567, which was isolated from a central venous catheter infection. Read More

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Effect of biofilm formation on implant abutments with an anti-bacterial coating: A pre-clinical in vivo study.

Clin Oral Implants Res 2021 Mar 13. Epub 2021 Mar 13.

Department of Periodontology, Institute of Odontology, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.

Objectives: To analyse the long-term effect of plaque formation on implant abutments with an antibacterial coating and the ensuing host response in peri-implant tissues.

Materials And Methods: Four implants were installed in each mandibular premolar region following tooth extraction in six dogs. Three months later, two test abutments with a titanium-bismuth-gallium (Ti-Bi-Ga) coating and two control titanium abutments were connected to the implants on each side of the mandible. Read More

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Osteointegration, antimicrobial and antibiofilm activity of orthopaedic titanium surfaces coated with silver and strontium-doped hydroxyapatite using a novel blasting process.

Drug Deliv Transl Res 2021 Apr 13;11(2):702-716. Epub 2021 Mar 13.

School of Pharmacy, University College Cork, Cork, Ireland.

Poor integration of orthopaedic devices with the host tissue owing to aseptic loosening and device-associated infections are two of the leading causes of implant failure, which represents a significant problem for both patients and the healthcare system. Novel strategies have focused on silver to combat antimicrobial infections as an alternative to drug therapeutics. In this study, we investigated the impact of increasing the % substitution (12% wt) of silver and strontium in hydroxyapatite (HA) coatings to enhance antimicrobial properties and stimulate osteoblasts, respectively. Read More

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GraXRS-Dependent Resistance of Staphylococcus aureus to Human Osteoarthritic Synovial Fluid.

mSphere 2021 03 10;6(2). Epub 2021 Mar 10.

Department of Microbiology and Immunology, University of Western Ontario, London, Ontario, Canada

Osteoarthritis is the most prevalent joint disease in the United States, with many patients requiring surgical replacement of the affected joint. The number of joint arthroplasty procedures performed each year is increasing, and infection is a leading cause of implant failure. is the most frequently isolated organism associated with periprosthetic joint infections of the knee or hip, and due to the emergence of antibiotic-resistant strains, treatment options are limited. Read More

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Expression of virulence factors by Pseudomonas aeruginosa biofilm after bacteriophage infection.

Microb Pathog 2021 Mar 7;154:104834. Epub 2021 Mar 7.

Human Exposome and Infectious Diseases Network - HEID, School of Nursing of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, Brazil; Department of Restorative Dentistry, School of Dentistry of Ribeirão Preto, University of São Paulo, São Paulo, Brazil. Electronic address:

The use of bacteriophages for the treatment of bacterial infections has been extensively studied. Nonetheless, the stress response regarding bacteriophage infection and the expression of virulence factors of Pseudomonas aeruginosa after phage infection is poorly discussed. In this study, we evaluated biofilm formation capacity and expression of virulence factors of P. Read More

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A Newly Characterized Potentially Probiotic Strain, Lactobacillus brevis MK05, and the Toxicity Effects of its Secretory Proteins Against MCF-7 Breast Cancer Cells.

Probiotics Antimicrob Proteins 2021 Mar 9. Epub 2021 Mar 9.

Department of Energy & Environmental Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran.

Among seven strains of lactic acid bacteria (LAB) isolated from traditional dairy products, a Lactobacillus strain was identified through 16S rRNA gene sequencing and tentatively designated as Lactobacillus brevis MK05. This strain demonstrated the highest probiotic potential through biochemical analysis, including acid and bile salt resistance, as well as antibacterial activity. The collected cell-free supernatant (CFC) of L. Read More

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How I Diagnose Anaplastic Large Cell Lymphoma.

Am J Clin Pathol 2021 03;155(4):479-497

Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN, USA.

Objectives: This review describes our approach to the diagnosis of all 4 anaplastic large cell lymphoma (ALCL) entities.

Methods: ALCLs are a group of CD30-positive mature T-cell lymphomas with similar morphologic and phenotypic characteristics but variable clinical and genetic features. They include systemic ALK-positive ALCL, systemic ALK-negative ALCL, primary cutaneous ALCL, and the recently described provisional entity breast implant-associated ALCL. Read More

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Investigations of Hydroxyapatite/Co-polymeric Composites Coated Titanium Plate for Bone Regeneration.

Front Cell Dev Biol 2020 18;8:631107. Epub 2021 Feb 18.

Department of Orthopedics, The Second Affiliated Hospital of Air Force Military Medical University, Xi'an, China.

A perfect mimic of human bone is very difficult. Still, the latest advancement in biomaterials makes it possible to design composite materials with morphologies merely the same as that of bone tissues. In the present work is the fabrication of selenium substituted Hydroxyapatite (HAP-Se) covered by lactic acid (LA)-Polyethylene glycol (PEG)-Aspartic acid (AS) composite with the loading of vincristine sulfate (VCR) drug (HAP-Se/LA-PEG-AS/VCR) for twin purposes of bone regenerations. Read More

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February 2021