242,661 results match your criteria microbiological control


Valorization of lipidic food waste for enhanced biodiesel recovery through two-step conversion: A novel microalgae-integrated approach.

Bioresour Technol 2021 Sep 20;342:125966. Epub 2021 Sep 20.

New Energy and Environmental Laboratory (NEEL), School of Architecture and Civil Engineering, Chengdu University, Chengdu 610106, PR China; Botany and Microbiology Department, Faculty of Science, Tanta University, Tanta 31527, Egypt.

The present study designed an innovative route for two-step biodiesel recovery from lipidic food waste followed by microalgae cultivation. Optimization of oil conversion showed the highest fatty acid methyl esters (FAMEs) recovery of 92.6% (lipid basis). Read More

View Article and Full-Text PDF
September 2021

Sublethal effects of chlorantraniliprole on Paederus fuscipes (Staphylinidae: Coleoptera), a general predator in paddle field.

Environ Pollut 2021 Sep 13;291:118171. Epub 2021 Sep 13.

Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, PR China.

Paederus fuscipes is a general predator in rice fields and a non-target organism of chlorantraniliprole, an effective insecticide for insect-pest control in paddy fields. Pesticide hazards to non-target organisms have been a growing global problem for decades. This study was designed to evaluate the toxicity of chlorantraniliprole at lethal and sublethal levels against P. Read More

View Article and Full-Text PDF
September 2021

A whole transcriptomic approach provides novel insights into the molecular basis of organophosphate and pyrethroid resistance in Anopheles arabiensis from Ethiopia.

Insect Biochem Mol Biol 2021 Sep 22:103655. Epub 2021 Sep 22.

Entomology Branch, Division of Parasitic Diseases and Malaria, Center for Global Health, U.S. Centers for Disease Control and Prevention, 1600 Clifton Rd. Atlanta, GA, 30329, USA. Electronic address:

The development of insecticide resistance in malaria vectors is of increasing concern in Ethiopia because of its potential implications for vector control failure. To better elucidate the specificity of resistance mechanisms and to facilitate the design of control strategies that minimize the likelihood of selecting for cross-resistance, a whole transcriptomic approach was used to explore gene expression patterns in a multi-insecticide resistant population of Anopheles arabiensis from Oromia Region, Ethiopia. This field population was resistant to the diagnostic doses of malathion (average mortality of 71. Read More

View Article and Full-Text PDF
September 2021

Syndromic approach to SARS-CoV-2 detection using QIAstat-Dx SARS-CoV-2 Panel from clinical samples.

J Virol Methods 2021 Sep 22:114300. Epub 2021 Sep 22.

Department of Clinical Virology, Institute of Liver & Biliary Sciences, New Delhi, India. Electronic address:

The QIAstat-Dx SARS-CoV-2 panel is a multiplex cartridge based assay based on real time PCR which can detect 17 respiratory viruses, including the novel coronavirus SARS-CoV-2. A syndromic approach is the need of the hour for COVID-19 diagnostics among patients presenting with respiratory symptoms. The present study was done to evaluate 120 archived respiratory clinical specimens for SARS-CoV-2 on the SARS-CoV-2 panel. Read More

View Article and Full-Text PDF
September 2021

Efficacy of lactic acid bacteria supplementation against Fusarium graminearum growth in vitro and inhibition of Zearalenone causing inflammation and oxidative stress in vivo.

Toxicon 2021 Sep 22. Epub 2021 Sep 22.

Laboratory of Genetic, Biodiversity and Bio-resources Valorisation, University of Monastir, Monastir, Tunisia; Higher Institute of Biotechnology of Béja, University of Jendouba, Jendouba, Tunisia. Electronic address:

Fusarium graminearum invasion and Zearalenone (ZEN)-mycotoxin contamination are considered the most global threat to food and feed. This study investigates the effect Lactobacillus plantarum MON03 viable cells (LPVC) and LP free cells supernatant (LPFCS) against Fusarium graminearum growth and ZEN production in vitro and evaluates if treatment with LP viable cells can counteract the negative effect of ZEN on inflammation and oxidative stress in mesenteric lymph nodes and serum biochemical parameters in mice. For the in vitro study, 7 days of LPVC, LPFCS and F. Read More

View Article and Full-Text PDF
September 2021

Immunity after COVID-19 and vaccination: follow-up study over 1 year among medical personnel.

Infection 2021 Sep 25. Epub 2021 Sep 25.

Institute for Clinical Microbiology and Hygiene, University Hospital Regensburg, Regensburg, Germany.

Background: The long-term course of immunity among individuals with a history of COVID-19, in particular among those who received a booster vaccination, has not been well defined so far.

Methods: SARS-CoV-2-specific antibody levels were measured by ELISA over 1 year among 136 health care workers infected during the first COVID-19 wave and in a subgroup after booster vaccination approximately 1 year later. Furthermore, spike-protein-reactive memory T cells were quantified approximately 7 months after the infection and after booster vaccination. Read More

View Article and Full-Text PDF
September 2021

Protocatechuic acid abrogates oxidative insults, inflammation, and apoptosis in liver and kidney associated with monosodium glutamate intoxication in rats.

Environ Sci Pollut Res Int 2021 Sep 25. Epub 2021 Sep 25.

Department of Zoology and Entomology, Helwan University, Cairo, 11795, Egypt.

Monosodium glutamate (MSG), a commonly used flavor enhancer, has been reported to induce hepatic and renal dysfunctions. In this study, the palliative role of protocatechuic acid (PCA) in MSG-administered rats was elucidated. Adult male rats were assigned to four groups, namely control, MSG (4 g/kg), PCA (100 mg/kg), and the last group was co-administered MSG and PCA at aforementioned doses for 7 days. Read More

View Article and Full-Text PDF
September 2021

Association of Maternal Diabetes and Autism Spectrum Disorders in Offspring: a Study in a Rodent Model of Autism.

J Mol Neurosci 2021 Sep 25. Epub 2021 Sep 25.

Central Laboratory, Female Campus, King Saud University, Riyadh, Saudi Arabia.

The present study investigated that maternal type 1 diabetes may contribute to autism pathogenesis in offspring, and that insulin therapy during pregnancy may prevent the onset of autism. As evidenced, selected brain biomarkers representing the accepted etiological mechanism of autism in newborn rats from diabetic mothers and diabetic mothers receiving insulin therapy compared to the propionic acid (PPA) rodent model of autism were screened. Female Wistar rats with a controlled fertility cycle were randomly divided into three groups: a control group, a group treated with a single dose of 65 mg/kg streptozotocin (STZ) to induce type 1 diabetes (T1D), and a group treated with a single dose of STZ to induce T1D along with insulin therapy. Read More

View Article and Full-Text PDF
September 2021

Cohnella cholangitidis sp. nov., a novel species of the genus Cohnella isolated from a clinical specimen in Korea.

Arch Microbiol 2021 Sep 25. Epub 2021 Sep 25.

Division of Pathogen Resource Management, Center for Public Vaccine Development Support, National Institute of Health, Korea Disease Control and Prevention Agency (KDCA), Cheongju, Republic of Korea.

A Gram-positive, aerobic, rod-shaped bacterium, designated as strain 1605-214, was isolated from the blood sample of a patient with cholangitis. Based on its 16S rRNA gene sequence, the strain 1605-214 belonged to the genus Cohnella and exhibited 97.9% sequence identity with Cohnella luojiensis DSM 24270 (GQ214052). Read More

View Article and Full-Text PDF
September 2021

Genome-wide transcriptional response of the causal soybean sudden death syndrome pathogen Fusarium virguliforme to a succinate dehydrogenase inhibitor fluopyram.

Pest Manag Sci 2021 Sep 24. Epub 2021 Sep 24.

Department of Plant, Soil and Microbial Sciences, Michigan State University, East Lansing, MI, USA.

Background: Succinate dehydrogenase inhibitors (SDHIs) have been widely used to manage plant diseases caused by phytopathogenic fungi. Although attention to and use of SDHI fungicides has recently increased, molecular responses of fungal pathogens to SDHIs have often not been investigated. A SDHI fungicide, fluopyram, has been used as a soybean seed treatment and has displayed effective control of Fusarium virguliforme, one of the causal agents of soybean sudden death syndrome. Read More

View Article and Full-Text PDF
September 2021

Occurrence of urogenital mycoplasmas in men with the common genitourinary diseases.

Braz J Microbiol 2021 Sep 24. Epub 2021 Sep 24.

Med Holding Emil Michalowski Specialist Hospital, Katowice, Poland.

Many serious and fatal infections with urogenital mycoplasmas in immunocompromised patients have been reported. M. genitalium is recognized as a cause of male urethritis and other common genitourinary diseases. Read More

View Article and Full-Text PDF
September 2021

An Anecdote on Prospective Protein Targets for Developing Novel Plant Growth Regulators.

Mol Biotechnol 2021 Sep 25. Epub 2021 Sep 25.

Department of Microbiology & Biotechnology, University School of Sciences, Gujarat University, Ahmedabad, Gujarat, 380009, India.

Phytohormones are the main regulatory molecules of core signalling networks associated with plant life cycle regulation. Manipulation of hormone signalling cascade enables the control over physiological traits of plant, which has major applications in field of agriculture and food sustainability. Hence, stable analogues of these hormones are long sought after and many of them are currently known, but the quest for more effective, stable and economically viable analogues is still going on. Read More

View Article and Full-Text PDF
September 2021

The return of chloroquine-sensitive Plasmodium falciparum parasites in Jazan region, southwestern Saudi Arabia over a decade after the adoption of artemisinin-based combination therapy: analysis of genetic mutations in the pfcrt gene.

Parasitol Res 2021 Sep 25. Epub 2021 Sep 25.

Medical Research Centre, Jazan University, Jazan, Kingdom of Saudi Arabia.

This study investigated the polymorphism in the P. falciparum chloroquine resistance transporter (pfcrt) gene 11 years after chloroquine (CQ) cessation in Jazan region, southwestern Saudi Arabia. Two hundred and thirty-five P. Read More

View Article and Full-Text PDF
September 2021

Moringa oleifera seeds-removed ripened pods as alternative for papersheet production: antimicrobial activity and their phytoconstituents profile using HPLC.

Sci Rep 2021 Sep 24;11(1):19027. Epub 2021 Sep 24.

Department of Engineering, University of Exeter, Exeter, EX4 4QF, UK.

In the present study, and for the waste valorization, Moringa oleifera seeds-removed ripened pods (SRRP) were used for papersheet production and for the extraction of bioactive compounds. Fibers were characterized by SEM-EDX patterns, while the phytoconstituents in ethanol extract was analyzed by HPLC. The inhibition percentage of fungal mycelial growth (IFMG) of the treated Melia azedarach wood with M. Read More

View Article and Full-Text PDF
September 2021

Antibody-dependent cellular cytotoxicity response to SARS-CoV-2 in COVID-19 patients.

Signal Transduct Target Ther 2021 Sep 24;6(1):346. Epub 2021 Sep 24.

Division of HIV/AIDS and Sex-transmitted Virus Vaccines, Institute for Biological Product Control, National Institutes for Food and Drug Control (NIFDC), Beijing, China.

Antibody-dependent cellular cytotoxicity (ADCC) responses to viral infection are a form of antibody regulated immune responses mediated through the Fc fragment. Whether severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) triggered ADCC responses contributes to COVID-19 disease development is currently not well understood. To understand the potential correlation between ADCC responses and COVID-19 disease development, we analyzed the ADCC activity and neutralizing antibody response in 255 individuals ranging from asymptomatic to fatal infections over 1 year post disease. Read More

View Article and Full-Text PDF
September 2021

Selective Induction of Cell Death in Human M1 Macrophages by Smac Mimetics Is Mediated by cIAP-2 and RIPK-1/3 through the Activation of mTORC.

J Immunol 2021 Sep 24. Epub 2021 Sep 24.

Department of Biochemistry, Microbiology, and Immunology, University of Ottawa, Ontario, Canada;

Inflammatory macrophages have been implicated in many diseases, including rheumatoid arthritis and inflammatory bowel disease. Therefore, targeting macrophage function and activation may represent a potential strategy to treat macrophage-associated diseases. We have previously shown that IFN-γ-induced differentiation of human M0 macrophages toward proinflammatory M1 state rendered them highly susceptible to the cytocidal effects of second mitochondria-derived activator of caspases mimetics (SMs), antagonist of the inhibitors of apoptosis proteins (IAPs), whereas M0 and anti-inflammatory M2c macrophages were resistant. Read More

View Article and Full-Text PDF
September 2021

CXCL17 Is Dispensable during Hypervirulent HN878 Infection in Mice.

Immunohorizons 2021 Sep 24;5(9):752-759. Epub 2021 Sep 24.

Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, MO;

CXCL17 is a novel mucosal chemokine that mediates myeloid cell recruitment and bactericidal activity and highly expressed in the respiratory tract. However, its role in tuberculosis (TB) immunopathogenesis or protection remains unknown. In this study, we evaluated the function of CXCL17 in a mouse model of aerosol infection with the clinical W-Beijing lineage hypervirulent HN878 strain. Read More

View Article and Full-Text PDF
September 2021

The implementation and outcome of a 2-year prospective audit and feedback based antimicrobial stewardship program at a private tertiary care hospital.

Indian J Med Microbiol 2021 Sep 21. Epub 2021 Sep 21.

Infection Control Nurse, Kokilaben Dhirubhai Ambani Hospital and Medical Research Institute, Four Bungalows, Andheri West, Mumbai, 400 053, Maharashtra, India.

Purpose: Antimicrobial resistance has emerged as a major public health problem in India. Hence effective antimicrobial stewardship programs (AMSP) are needed. We report the design, implementation and results of a prospective audit and feedback based AMSP at a private tertiary care hospital. Read More

View Article and Full-Text PDF
September 2021

Ginkgo biloba extract (EGb 761) mitigates methotrexate-induced testicular insult in rats: Targeting oxidative stress, energy deficit and spermatogenesis.

Biomed Pharmacother 2021 Sep 21;143:112201. Epub 2021 Sep 21.

Department of Biochemistry, Faculty of Pharmacy and Biotechnology, German University in Cairo, Cairo, Egypt.

Methotrexate (MTX) is commonly used as a therapeutic agent in the treatment of malignancies and autoimmune disorders. Risk of subsequent infertility following MTX administration has been reported as a significant side effect due to testicular toxicity. The aim of the study was to evaluate the modulatory effects of Ginkgo biloba (standardized extract, EGb 761) on MTX-induced testicular oxidative stress, energy deficits and spermatogenic status in rats. Read More

View Article and Full-Text PDF
September 2021

Human herpesvirus-encoded MicroRNA in host-pathogen interaction.

Adv Biol Regul 2021 Sep 16;82:100829. Epub 2021 Sep 16.

Department of Periodontics, College of Dentistry, University of Illinois at Chicago, United States. Electronic address:

Human herpesviruses (HHV) are ubiquitous, linear dsDNA viruses that establish lifelong latency, disrupted by sporadic reactivation. HHV have evolved diverse ingenious mechanisms to evade robust host defenses. Incorporation of unique stem loop sequences that generate viral microRNAs (v-miRs) exemplifies one such evolutionary adaptation in HHV. Read More

View Article and Full-Text PDF
September 2021

Reverse transcription recombinase-aided amplification assay for rapid detection of the influenza A(H1N1)pdm09 H275Y mutation that confers oseltamivir resistance.

Mol Cell Probes 2021 Sep 21:101771. Epub 2021 Sep 21.

Microbiology Laboratory, Hangzhou Centre for Disease Control and Prevention, Zhejiang, 310021, China. Electronic address:

The emergence of the influenza A(H1N1)pdm09 virus with the NA-H275Y mutation, which confers oseltamivir resistance, must be monitored, especially in patients undergoing neuraminidase inhibitor treatment. In this study, we developed a reverse transcription recombinase-aided amplification assay that has high sensitivity (detection limit: 1.0×10 copies/μL) and specificity for detecting the oseltamivir-resistant H275Y mutation; the assay is performed within 30 minutes at a constant temperature of 39 degrees Celsius using an isothermal device. Read More

View Article and Full-Text PDF
September 2021

The effects of quorum sensing molecule farnesol on the yield and activity of extracellular polysaccharide from Grifola frondosa in liquid fermentation.

Int J Biol Macromol 2021 Sep 21. Epub 2021 Sep 21.

Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, State Key Laboratory of Food Nutrition and Safety, College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, PR China. Electronic address:

A strategy by exogenous addition of quorum sensing molecule farnesol to improve the production, antioxidant activity and antitumor activity of extracellular polysaccharide (EPS) of Grifola frondosa by liquid fermentation was proposed in the study. The highest yield of EPS induced by farnesol was 1.25 g/L, which was 150% higher than that of the control. Read More

View Article and Full-Text PDF
September 2021

Quantitative characterization of the B cell receptor repertoires of human immunized with commercial rabies virus vaccine.

Hum Vaccin Immunother 2021 Aug;17(8):2538-2546

Laboratory for Diagnosis of Clinical Microbiology and Infection, Yuebei People's Hospital, Shantou University Medical College, Shaoguan, China.

Humoral immunity is crucial for an efficient host immune response against rabies virus (RABV) infection. But the B cell receptor (BCR) repertoire in human after RABV vaccine immunization remained unclear. To study the BCR repertoires in peripheral blood mononuclear cells (PBMCs) of human immunized with rabies virus vaccine. Read More

View Article and Full-Text PDF

Every Country, Every Woman, Every Child; Group B Streptococcal Disease Worldwide Prematurity modifies the risk of long-term neurodevelopmental impairments after invasive Group B Streptococcus infections during infancy in Denmark and the Netherlands.

Clin Infect Dis 2021 Sep 24. Epub 2021 Sep 24.

Amsterdam UMC, University of Amsterdam, Department of Neurology, Amsterdam Neuroscience, Meibergdreef 9, 1100 DD Amsterdam, The Netherlands.

Background: Preterm birth and neonatal infections are both associated with mortality and long-term neurodevelopmental impairments (NDI). We examined whether the effect of invasive group B streptococcus disease (iGBS) on mortality and long-term NDI differs for preterm and term infants, and whether co-occurrence of iGBS and prematurity leads to worse outcome.

Methods: Two nationwide cohort studies of children with a history of iGBS were conducted using Danish and Dutch medical and administrative databases. Read More

View Article and Full-Text PDF
September 2021

Livestock Presence Influences the Seroprevalence of Crimean Congo Hemorrhagic Fever Virus on Sympatric Wildlife in Kenya.

Vector Borne Zoonotic Dis 2021 Sep 24. Epub 2021 Sep 24.

Department of Clinical Microbiology, Umeå University, Umeå, Sweden.

Crimean Congo Hemorrhagic Fever (CCHF) is an emerging tick-borne zoonotic viral disease with the potential of causing public health emergencies. However, less is known about the role of wildlife and livestock in spreading the virus. Therefore, we aimed to assess how the interactions between African buffalo () and cattle may influence the seroprevalence of CCHF across livestock-wildlife management systems in Kenya. Read More

View Article and Full-Text PDF
September 2021

Capsid Allosteric Modulators Enhance the Innate Immune Response in Hepatitis B Virus-Infected Hepatocytes During Interferon Administration.

Hepatol Commun 2021 Aug 25. Epub 2021 Aug 25.

Department of Gastroenterology and Hepatology, Osaka University Graduate School of Medicine, Osaka, Japan.

Capsid allosteric modulators (CAMs) inhibit the encapsidation of hepatitis B virus (HBV) pregenomic RNA (pgRNA), which contains a pathogen-associated molecular pattern motif. However, the effect of CAMs on the innate immune response of HBV-infected hepatocytes remains unclear, and we examined this effect in this study. Administration of a CAM compound, BAY41-4109 (BAY41), to HBV-infected primary human hepatocytes (PHHs) did not change the total cytoplasmic pgRNA levels but significantly reduced intracapsid pgRNA levels, suggesting that BAY41 increased extracapsid pgRNA levels in the cytoplasm. Read More

View Article and Full-Text PDF

Ipragliflozin Improves the Hepatic Outcomes of Patients With Diabetes with NAFLD.

Hepatol Commun 2021 Jun 17. Epub 2021 Jun 17.

Division of Metabolism and Endocrinology, Faculty of Medicine, Saga University, Saga, Japan.

Sodium glucose cotransporter-2 inhibitors (SGLT2is) are now widely used to treat diabetes, but their effects on nonalcoholic fatty liver disease (NAFLD) remain to be determined. We aimed to evaluate the effects of SGLT2is on the pathogenesis of NAFLD. A multicenter, randomized, controlled trial was conducted in patients with type 2 diabetes with NAFLD. Read More

View Article and Full-Text PDF

IMB-BZ as an Inhibitor Targeting ESX-1 Secretion System to Control Mycobacterial Infection.

J Infect Dis 2021 Sep 24. Epub 2021 Sep 24.

Institute of Medicinal Biotechnology Chinese Academy of Medical Sciences, Beijing, 100050, China.

Background: Resistance to anti-tuberculosis (TB) drug is a major issue in TB control, and demands the discovery of new drugs targeting virulence factor ESX-1.

Methods: We first established a high-throughput screen (HTS) assay for the discovery of ESX-1 secretion inhibitors. The positive hits were then evaluated for the potency of diminishing the survival of virulent mycobacterium and reducing bacterial virulence. Read More

View Article and Full-Text PDF
September 2021

Cholera pulse vaccination: A possible elimination strategy for cholera in endemic countries.

Indian J Public Health 2021 Jul-Sep;65(3):311-314

Senior Resident, Department of Medical Microbiology, Postgraduate Institute of Medical Education and Research, Chandigarh, India.

Cholera is a diarrheal disease causing major health issue in developing countries where it is endemic and causes outbreaks. India ranks first with an estimated 675,188 number of cases and 20,256 number of deaths annually with one-third of its population at risk. The two broad approaches for cholera control are improving sanitation and vaccination. Read More

View Article and Full-Text PDF
September 2021

Threat in water for drinking and domestic use: Nontuberculous mycobacteria.

Int J Mycobacteriol 2021 Apr-Jun;10(2):188-192

Faculty of Medicine, Department of Biostatistics and Medical Informatics, Düzce University, Düzce, Turkey.

Objective: Nontuberculous mycobacteria (NTM) have been recognized as a diverse group of organisms that are ubiquitous in environmental sources. In most regions of the world, NTM are not reportable as a public health disease, so epidemiological data are not easily available. However, data in published studies note increasing trends at the rate of NTM isolation from different geographic regions of the world. Read More

View Article and Full-Text PDF
September 2021