88,215 results match your criteria mice exhibited

Artificial M2 macrophages for disease-modifying osteoarthritis therapeutics.

Biomaterials 2021 May 3;274:120865. Epub 2021 May 3.

Key Laboratory of Biomaterials of Guangdong Higher Education Institutes, Engineering Technology Research Center of Drug Carrier of Guangdong, Department of Biomedical Engineering Jinan University Guangzhou, 510632, China. Electronic address:

Osteoarthritis (OA) is one of the most common joint diseases worldwide and the focus is shifting to disease prevention and the pharmaceutical and surgical treatment of early OA. However, at present few have proven ability to block or delay the progression of OA. Nevertheless, M2 macrophages present an anti-inflammatory function and promote cartilage repair, thereby alleviating OA in mice. Read More

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Design, synthesis, in vitro and in vivo evaluation against MRSA and molecular docking studies of novel pleuromutilin derivatives bearing 1, 3, 4-oxadiazole linker.

Bioorg Chem 2021 May 1;112:104956. Epub 2021 May 1.

Guangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China; Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou, 510642, China. Electronic address:

A class of pleuromutilin derivatives containing 1, 3, 4-oxadiazole were designed and synthesized as potential antibacterial agents against Methicillin-resistant staphylococcus aureus (MRSA). The ultrasound-assisted reaction was proposed as a green chemistry method to synthesize 1, 3, 4-oxadiazole derivatives (intermediates 85-110). Among these pleuromutilin derivatives, compound 133 was found to be the strongest antibacterial derivative against MRSA (MIC = 0. Read More

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Cervical spinal cord injury-induced neuropathic pain in male mice is associated with a persistent pro-inflammatory macrophage/microglial response in the superficial dorsal horn.

Exp Neurol 2021 May 12:113757. Epub 2021 May 12.

Department of Neuroscience, Vickie and Jack Farber Institute for Neuroscience, Sidney Kimmel Medical College at Thomas Jefferson University, 233 South 10th Street, BLSB 245, Philadelphia, PA 19107, United States. Electronic address:

A significant portion of individuals living with traumatic spinal cord injury (SCI) experiences some degree of debilitating neuropathic pain (NP). This pain remains largely intractable in a majority of cases, due in part to an incomplete understanding of its underlying mechanisms. Central sensitization, an increase in excitability of pain transmission neurons located in superficial dorsal horn (sDH), plays a key role in development and maintenance of SCI-induced NP. Read More

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Hippocampal Interneurons are Required for Trace Eyeblink Conditioning in Mice.

Neurosci Bull 2021 May 15. Epub 2021 May 15.

Experimental Center of Basic Medicine, College of Basic Medical Sciences, Army Medical University, Chongqing, 400038, China.

While the hippocampus has been implicated in supporting the association among time-separated events, the underlying cellular mechanisms have not been fully clarified. Here, we combined in vivo multi-channel recording and optogenetics to investigate the activity of hippocampal interneurons in freely-moving mice performing a trace eyeblink conditioning (tEBC) task. We found that the hippocampal interneurons exhibited conditioned stimulus (CS)-evoked sustained activity, which predicted the performance of conditioned eyeblink responses (CRs) in the early acquisition of the tEBC. Read More

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IFN-alpha receptor deficiency enhances host resistance to oral Salmonella enterica serovar Typhimurium infection during murine pregnancy.

Am J Reprod Immunol 2021 May 15:e13454. Epub 2021 May 15.

Division of Life Sciences, Human Health Therapeutics, National Research Council Canada, Ottawa, ON, Canada, K1A 0R6.

Problem: Maternal tolerance during pregnancy increases the risk of infection with certain intracellular pathogens. Systemic Salmonella enterica serovar Typhimurium (S.Tm) infection during pregnancy in normally-resistant 129X1/SvJ mice leads to severe placental infection, fetal and maternal death. Read More

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Serum exosomes accelerate diabetic wound healing by promoting angiogenesis and ECM formation.

Cell Biol Int 2021 May 14. Epub 2021 May 14.

Department of Guangdong Provincial Key Laboratory of Pharmaceutical Bioactive Substances, School of Life Sciences and Biopharmaceuticals, Guangdong Pharmaceutical University, Guangzhou, 510006, Guangdong Province, China.

Non-healing wounds in diabetes remain a global clinical and research challenge. Exosomes are primary mediators of cell paracrine action, which are shown to promote tissue repair and regeneration. In this study, we investigated the effects of serum derived exosomes (Serum-Exos) on diabetic wound healing and its possible mechanisms. Read More

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Alternative regulatory mechanism for the maintenance of bone homeostasis via STAT5-mediated regulation of the differentiation of BMSCs into adipocytes.

Exp Mol Med 2021 May 14. Epub 2021 May 14.

Department of Pharmacology, Chonnam National University Medical School, Gwangju, 61469, Republic of Korea.

STAT5 is a transcription factor that is activated by various cytokines, hormones, and growth factors. Activated STAT5 is then translocated to the nucleus and regulates the transcription of target genes, affecting several biological processes. Several studies have investigated the role of STAT5 in adipogenesis, but unfortunately, its role in adipogenesis remains controversial. Read More

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Omega-3 fatty acid-rich fish oil supplementation prevents rosiglitazone-induced osteopenia in aging C57BL/6 mice and in vitro studies.

Sci Rep 2021 May 14;11(1):10364. Epub 2021 May 14.

Department of Biological and Environmental Sciences, Qatar University, Doha, Qatar.

Rosiglitazone is an effective insulin-sensitizer, however associated with bone loss mainly due to increased bone resorption and bone marrow adiposity. We investigated the effect of the co-administration of fish oil rich in omega-3 fatty acids (FAs) on rosiglitazone-induced bone loss in C57BL/6 mice and the mechanisms underlying potential preventive effect. Mice fed the iso-caloric diet supplemented with fish oil exhibited significantly higher levels of bone density in different regions compared to the other groups. Read More

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The endoribonuclease N4BP1 prevents psoriasis by controlling both keratinocytes proliferation and neutrophil infiltration.

Cell Death Dis 2021 May 14;12(5):488. Epub 2021 May 14.

Department of Pathogenic Biology, School of Medicine, Nantong University, 226001, Jiangsu, China.

Psoriasis is a common chronic skin disease, characterized by abnormal interplay between hyperproliferative epidermal keratinocytes and self-reactive immune cells with not fully addressed molecular mechanism. N4BP1 (NEDD4-binding protein 1) is considered as an immune regulator for a long time but its physiological role is not determined yet. Here, we found that the expression of N4BP1 in skin was highest among all 54 tested tissues, and its expression was further upregulated in psoriatic skin. Read More

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Targeting monoamine oxidase A for T cell-based cancer immunotherapy.

Sci Immunol 2021 May;6(59)

Department of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, CA 90095, USA.

Monoamine oxidase A (MAO-A) is an enzyme best known for its function in the brain, where it breaks down neurotransmitters and thereby influences mood and behavior. Small-molecule MAO inhibitors (MAOIs) have been developed and are clinically used for treating depression and other neurological disorders. However, the involvement of MAO-A in antitumor immunity has not been reported. Read More

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Core-binding factor beta is required for osteoblast differentiation during fibula fracture healing.

J Orthop Surg Res 2021 May 14;16(1):313. Epub 2021 May 14.

The Second Department of Orthopedics, Shaanxi Traditional Chinese Medicine Hospital, Xi'an, 710003, People's Republic of China.

Background: Growing evidence has implicated core-binding factor beta (Cbfb) as a contributor to osteoblast differentiation, which plays a key role in fracture healing. Herein, we aimed to assess whether Cbfb affects osteoblast differentiation after fibula fracture.

Methods: Initially, we established a Cbfb conditional knockout mouse model for subsequent studies. Read More

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The bone is the major source of high circulating intact fibroblast growth factor-23 in acute murine polymicrobial sepsis induced by cecum ligation puncture.

PLoS One 2021 14;16(5):e0251317. Epub 2021 May 14.

Department of Biomedical Sciences, University of Veterinary Medicine Vienna, Vienna, Austria.

Fibroblast growth factor-23 (FGF23), a bone-produced hormone, plays a critical role in mineral homeostasis. Human diseases associated with excessive intact circulating FGF23 (iFGF23) result in hypophosphatemia and low vitamin D hormone in patients with normal kidney function. In addition, there is accumulating evidence linking FGF23 with inflammation. Read More

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HG-9-91-01 Attenuates Murine Experimental Colitis by Promoting Interleukin-10 Production in Colonic Macrophages Through the SIK/CRTC3 Pathway.

Inflamm Bowel Dis 2021 May 14. Epub 2021 May 14.

State Key Laboratory of Analytical Chemistry for Life Science, School of Life Sciences, Nanjing University, Nanjing, China.

Background: Interleukin-10 (IL-10) is a potent immunoregulatory cytokine that plays a pivotal role in maintaining mucosal immune homeostasis. As a novel synthetic inhibitor of salt-inducible kinases (SIKs), HG-9-91-01 can effectively enhance IL-10 secretion at the cellular level, but its in vivo immunoregulatory effects remain unclear. In this study, we investigated the effects and underlying mechanism of HG-9-91-01 in murine colitis models. Read More

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Carboxymethyl konjac glucomannan coating on multilayered emulsions for improved bioavailability and targeted delivery of curcumin.

Food Funct 2021 May 14. Epub 2021 May 14.

College of Food Science and Engineering, Qingdao Agricultural University, Qingdao 266109, PR China.

Curcumin was entrapped in multilayered emulsions to increase its stability and bioavailability. Curcumin emulsion stabilized by whey protein isolate (WPI) was coated with chitosan (CHI) or carboxymethyl konjac glucomannan (CMKGM) alone to form secondary emulsions and their combination in sequence to form the tertiary emulsion, in which, the polyelectrolyte concentrations were 1.0% WPI for the primary emulsion, 0. Read More

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Adult neurogenic process in the subventricular zone-olfactory bulb system is regulated by Tau protein under prolonged stress.

Cell Prolif 2021 May 14:e13027. Epub 2021 May 14.

School of Medicine, Life and Health Sciences Research Institute (ICVS), University of Minho, Braga, Portugal.

Objectives: The area of the subventricular zone (SVZ) in the adult brain exhibits the highest number of proliferative cells, which, together with the olfactory bulb (OB), maintains constant brain plasticity through the generation, migration and integration of newly born neurons. Despite Tau and its malfunction is increasingly related to deficits of adult hippocampal neurogenesis and brain plasticity under pathological conditions [e.g. Read More

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Prevention of Cholesterol Gallstone Formation by ATCC 43121 and MF27 in Lithogenic Diet-Induced Mice.

Food Sci Anim Resour 2021 Mar 1;41(2):343-352. Epub 2021 Mar 1.

Department of Food Bioscience and Technology, Korea University, Seoul 02841, Korea.

The objective of this study was to evaluate the effects of ATCC 43121 and MF27 on biochemical indices in the serum, cholesterol metabolism in the liver and mucin expression in the gallbladder in lithogenic diet (LD)-induced C57BL/6J mice to determine the preventive effects of lactobacilli on gallstone formation. By the end of 4 wk of the experimental period, mice fed on a LD with high-fat and high-cholesterol exhibited higher levels of total and low-density lipoprotein cholesterol in the serum compared to mice fed on control diet or LD with ATCC 43121 (LD+P1; p<0.05). Read More

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CD2 T-helper 17-like cells differentiated from a CD133 subpopulation of non-small cell lung carcinoma cells promote the growth of lung carcinoma.

Ann Transl Med 2021 Apr;9(8):687

Institute of Basic Medicine, Hebei Medical University, Shijiazhuang, China.

Background: Cancer stem cells (CSCs) give rise to a diverse variety of differentiated cells, which comprise the bulk of the tumor microenvironment (TME). However, the exact multi-directional differentiation potential of CSCs has not been fully clarified. This study was designed to explore whether CSCs differentiate into cellular components of the TME to promote the growth of lung carcinoma. Read More

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Diabetic nephropathy in mice is aggravated by the absence of podocyte IRE1 and is correlated with reduced kidney ADH1 expression.

Ann Transl Med 2021 Apr;9(8):636

Shanghai Diabetes Institute, Department of Endocrinology and Metabolism, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Background: Inositol-requiring enzyme 1 (IRE1) plays a critical role in attenuating endoplasmic reticulum (ER) stress associated with renal injury which may also be a factor in diabetic nephropathy (DN). Alcohol dehydrogenase type I (ADH1) activity is prominent in the kidney, ADH1 activity is also reported to exert protective effects against ER stress that are not caused by alcohol consumption. However, the role of IRE1 in DN and the correlation between IRE1 and ADH1 activity remain unclear. Read More

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Endothelial TLR2 promotes proangiogenic immune cell recruitment and tumor angiogenesis.

Sci Signal 2021 Jan;14(666)

Department of Neuroscience, Lerner Research Institute, Cleveland Clinic, OH, USA 44195.

Toll-like receptor 2 (TLR2) is implicated in various pathologies, mainly in terms of its function within innate immune cells. However, TLR2 is also present in endothelial cells. Here, we explored the physiological and pathophysiological roles of endothelial TLR2 signaling. Read More

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January 2021

miR-9-5p regulates immunometabolic and epigenetic pathways in β-glucan-trained immunity via IDH3α.

JCI Insight 2021 May 10;6(9). Epub 2021 May 10.

State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Science, Fudan University, Shanghai, China.

Trained immunity, induced by β-glucan in monocytes, is mediated by activating metabolic pathways that result in epigenetic rewiring of cellular functional programs; however, molecular mechanisms underlying these changes remain unclear. Here, we report a key immunometabolic and epigenetic pathway mediated by the miR-9-5p-isocitrate dehydrogenase 3α (IDH3α) axis in trained immunity. We found that β-glucan-trained miR-9-5p-/- monocytes showed decreased IL-1β, IL-6, and TNF-α production after LPS stimulation. Read More

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Myocardial Lipin 1 knockout in mice approximates cardiac effects of human LPIN1 mutations.

JCI Insight 2021 May 10;6(9). Epub 2021 May 10.

Division of Geriatrics and Nutritional Science, Department of Medicine, Washington University School of Medicine, St. Louis, Missouri, USA.

Lipin 1 is a bifunctional protein that is a transcriptional regulator and has phosphatidic acid (PA) phosphohydrolase activity, which dephosphorylates PA to generate diacylglycerol. Human lipin 1 mutations lead to episodic rhabdomyolysis, and some affected patients exhibit cardiac abnormalities, including exercise-induced cardiac dysfunction and cardiac triglyceride accumulation. Furthermore, lipin 1 expression is deactivated in failing heart, but the effects of lipin 1 deactivation in myocardium are incompletely understood. Read More

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A resveratrol-loaded nanostructured lipid carrier hydrogel to enhance the anti-UV irradiation and anti-oxidant efficacy.

Colloids Surf B Biointerfaces 2021 Apr 27;204:111786. Epub 2021 Apr 27.

Department of Pharmaceutics, School of Pharmacy, Fourth Military Medical University, Xi'an, 710032, China; Key Laboratory of Gastrointestinal Pharmacology of Chinese Materia Medica of the State Administration of Traditional Chinese Medicine, Fourth Military Medical University, Xi'an, 710032, China. Electronic address:

Exposure to ultraviolet (UV) irradiation leads to the generation of reactive oxygen species (ROS) and DNA damage in skin tissue, which can further result in skin cancers. Using sunscreens is one of the most popular and the most effective method to resist UV irradiation. Resveratrol (RES) shows high absorbance in UV region and significant anti-oxidant effects. Read More

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Modulation of CXCR1 and CXCR3 expression on NK cells via Tim-3 in a murine model of primary biliary cholangitis.

Mol Immunol 2021 May 10;135:342-350. Epub 2021 May 10.

Department of Gastroenterology, The Second Affiliated Hospital of Kunming Medical University, Kunming, 650101 Yunnan Province, China; Yunnan Research Center for Liver Diseases, Kunming, 650101 Yunnan Province, China. Electronic address:

Tim-3, which is expressed on a variety of innate immune cells including NK cells, plays a key role in many autoimmune diseases. However, the immunomodulatory actions of Tim-3 on NK cells in primary biliary cholangitis (PBC) remain uncertain. Using a murine model of PBC we evaluated the expression of Tim-3 and its ligand Gal-9 in peripheral blood, liver, and spleen. Read More

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Radiolabeling and Evaluation of a Novel [TcN] Complex with Deferoxamine Dithiocarbamate as a Potential Agent for Bacterial Infection Imaging.

Bioorg Med Chem Lett 2021 May 10:128102. Epub 2021 May 10.

Key Laboratory of Radiopharmaceuticals of Ministry of Education, College of Chemistry, Beijing Normal University, Beijing 100875, P.R. China. Electronic address:

In order to find a Tc-labeled deferoxamine radiotracer for bacterial infection imaging, deferoxamine dithiocarbamate (DFODTC) was successfully synthesized and it was radiolabeled with [TcN] core to prepare the TcN(DFODTC) complex. TcN(DFODTC) was obtained with high radiochemical purity without further purification. The complex was lipophilic and exhibited good in vitro stability. Read More

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The impact of genetic background and sex on the phenotype of IL-23 induced murine spondyloarthritis.

PLoS One 2021 13;16(5):e0247149. Epub 2021 May 13.

Brigham and Women's Hospital, Boston, MA, United States of America.

Background: Overexpression of IL-23 in adult mice by means of hydrodynamic tail vein injection of IL-23 minicircles has been reported to result in spondyloarthritis-like disease. The impact of genetic background and sex on the disease phenotype in this model has not been investigated.

Methods: We compared male B10. Read More

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Inactivation of the CB receptor accelerated the neuropathological deterioration in TDP-43 transgenic mice, a model of amyotrophic lateral sclerosis.

Brain Pathol 2021 May 13:e12972. Epub 2021 May 13.

Departamento de Bioquímica y Biología Molecular, Facultad de Medicina, Instituto Universitario de Investigación en Neuroquímica, Universidad Complutense, Madrid, Spain.

The activation of the cannabinoid receptor type-2 (CB ) afforded neuroprotection in amyotrophic lateral sclerosis (ALS) models. The objective of this study was to further investigate the relevance of the CB receptor through investigating the consequences of its inactivation. TDP-43(A315T) transgenic mice were crossed with CB receptor knock-out mice to generate double mutants. Read More

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IL-34 reprograms glycolytic and osteoclastic RA macrophages via Syndecan-1 and M-CSFR.

Arthritis Rheumatol 2021 May 13. Epub 2021 May 13.

Jesse Brown VA Medical Center, Chicago, IL, 60612, USA.

Objective: In RA, IL-34 serum levels are linked with increased disease severity. IL-34 binds to two receptors, M-CSFR and Syndecan-1 (SDC-1), which are co-expressed in RA macrophages (MФs). Expression of both IL-34 and SDC-1 is strikingly elevated in the RA synovium, yet their mechanism of action remains undefined. Read More

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Exosomes from Adipose Mesenchymal Stem Cells Overexpressing Stanniocalcin-1 Promote Reendothelialization After Carotid Endarterium Mechanical Injury.

Stem Cell Rev Rep 2021 May 12. Epub 2021 May 12.

Department of Vascular Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200011, China.

Objective: Stanniocalcin-1 (STC-1) is a secreted glycoprotein that participates in the regulation of inflammation, apoptosis, and necrosis. We investigated the reendothelialization effect of exosomes from adipose stem cells (ADSC) overexpressing STC-1 on injured carotid endarterium.

Methods: ADSCs were transfected with lentivirus vectors containing pre-STC-1. Read More

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Inhibited HDAC3 or Elevated MicroRNA-494-3p Plays a Protective Role in Myocardial Ischemia-Reperfusion Injury via Suppression of BRD4.

Mol Neurobiol 2021 May 12. Epub 2021 May 12.

Department of Cardiology, The First Affiliated Hospital of Xiamen University, 55 Zhenhai Road, Siming District, Xiamen, 361000, Fujian, China.

Increased histone deacetylase 3 (HDAC3) has been demonstrated to contribute to the pathogenesis of myocardial ischemia-reperfusion injury (MI/RI). Therefore, the goal of this study was to investigate how HDAC3 regulated MI/RI by mediating microRNA (miR)-494-3p/dromodomain-containing protein 4 (BRD4) axis. The MI/RI model was established by ligating the right anterior descending coronary artery. Read More

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Neurobehavioral effects of acute low-dose whole-body irradiation.

J Radiat Res 2021 May 13. Epub 2021 May 13.

Department of Radiobiology and Hygiene Management, Institute of Industrial Ecological Sciences, University of Occupational and Environmental Health, Iseigaoka Yahatanishi-ku, Kitakyushu, Fukuoka 807-8555, Japan.

Radiation exposure has multiple effects on the brain, behavior and cognitive functions. It has been reported that high-dose (>20 Gy) radiation-induced behavior and cognitive aberration partly associated with severe tissue destruction. Low-dose (<3 Gy) exposure can occur in radiological disasters and cerebral endovascular treatment. Read More

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