49 results match your criteria mhy1485 activator

Fluoride regulates chondrocyte proliferation and autophagy via PI3K/AKT/mTOR signaling pathway.

Chem Biol Interact 2021 Sep 16;349:109659. Epub 2021 Sep 16.

Department of Environmental Health, School of Public Health, China Medical University, No. 77 Puhe Road, Shenyang North New Area, Shenyang, Liaoning Province, PR China. Electronic address:

Fluorine is an essential trace element for human health. However, excessive fluoride intake causes skeletal fluorosis which affects cartilage development. Fluoride inhibited chondrocyte proliferation which is the initial and critical step of endochondral ossification, but the underlying mechanism has not been clearly illustrated. Read More

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September 2021

L-Leucine Promotes STAT1 and ISGs Expression in TGEV-Infected IPEC-J2 Cells mTOR Activation.

Front Immunol 2021 22;12:656573. Epub 2021 Jul 22.

Key Laboratory for Animal Disease-Resistance Nutrition, Animal Nutrition Institute, Sichuan Agricultural University, Chengdu, China.

L-leucine (Leu), as one of the effective amino acids to activate the mTOR signaling pathway, can alleviate transmissible gastroenteritis virus (TGEV) infection. However, the underlying mechanism by which Leu alleviates the virus infection has not been fully characterized. In particular, how Leu impacts TGEV replication through mTOR signaling has yet to be elucidated. Read More

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Glucagon-like peptide-1 attenuates cardiac hypertrophy via the AngII/AT1R/ACE2 and AMPK/mTOR/p70S6K pathways.

Acta Biochim Biophys Sin (Shanghai) 2021 Aug;53(9):1189-1197

Department of General Medical, Shanxi Bethune Hospital, Shanxi Academy of Medical Sciences, Shanxi Medical University, Tongji Shanxi Hospital, Tongji Medical College, Huazhong University of Science and Technology, Taiyuan 030032, China.

Glucagon-like peptide-1 (GLP-1), a novel type of glucose-lowering agent, has been reported to exert cardioprotective effects. However, the cardioprotective mechanism of GLP-1 on spontaneous hypertension-induced cardiac hypertrophy has not been fully elucidated. In this study, we revealed that liraglutide or alogliptin treatment ameliorated spontaneous hypertension-induced cardiac hypertrophy, as evidenced by decreased levels of cardiac hypertrophic markers (atrial natriuretic peptide, brain natriuretic peptide, and β-myosin heavy chain), as well as systolic blood pressure, diastolic blood pressure, mean arterial pressure, and histological changes. Read More

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Knockdown of CNN3 Impairs Myoblast Proliferation, Differentiation, and Protein Synthesis via the mTOR Pathway.

Front Physiol 2021 8;12:659272. Epub 2021 Jul 8.

Guangdong Traditional Medical and Sports Injury Rehabilitation Research Institute, Guangdong Second Provincial General Hospital, Guangzhou, China.

Background: Myogenesis is a complex process that requires optimal outside-in substrate-cell signaling. Calponin 3 (CNN3) plays an important role in regulating myogenic differentiation and muscle regeneration; however, the precise function of CNN3 in myogenesis regulation remains poorly understood. Here, we investigated the role of CNN3 in a knockdown model in the mouse muscle cell line C2C12. Read More

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Bta-miR-34b controls milk fat biosynthesis via the Akt/mTOR signaling pathway by targeting RAI14 in bovine mammary epithelial cells.

J Anim Sci Biotechnol 2021 Jul 19;12(1):83. Epub 2021 Jul 19.

College of Animal Science and Technology, Northwest A&F University, Xianyang, 712100, Shaanxi, China.

Background: The biosynthesis of milk fat affects both the technological properties and organoleptic quality of milk and dairy products. MicroRNAs (miRNAs) are endogenous small non-coding RNAs that inhibit the expression of their mRNA targets and are involved in downstream signaling pathways that control several biological processes, including milk fat synthesis. miR-34b is a member of the miR-34 miRNA cluster, which is differentially expressed in the mammary gland tissue of dairy cows during lactation and dry periods. Read More

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MHY1485 enhances X-irradiation-induced apoptosis and senescence in tumor cells.

J Radiat Res 2021 Sep;62(5):782-792

Health and Medical Research Institute, Department of Life Science and Biotechnology, National Institute of Advanced Industrial Science and Technology (AIST), 2217-14, Hayashi-cho, Takamatsu, Kagawa 761-0895, Japan.

The mammalian target of rapamycin (mTOR) is a sensor of nutrient status and plays an important role in cell growth and metabolism. Although inhibition of mTOR signaling promotes tumor cell death and several mTOR inhibitors have been used clinically, recent reports have shown that co-treatment with MHY1485, an mTOR activator, enhances the anti-cancer effects of anti-PD-1 antibody and 5-fluorouracil. However, it remains unclear whether MHY1485 treatment alters the effects of radiation on tumor cells. Read More

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September 2021

Activation of Gonadotropin-releasing Hormone Receptor Impedes the Immunosuppressive Activity of Decidual Regulatory T Cells via Deactivating the Mechanistic Target of Rapamycin Signaling.

Immunol Invest 2021 Jun 16:1-17. Epub 2021 Jun 16.

Department of Preventive Medicine, School of Public Health, Fujian Medical University, Fuzhou, Fujian Province, People's Republic of China.

Understanding maternal immune tolerance is crucial for the development of therapeutics for immunological pregnancy complications. Decidual regulatory T cells (Tregs) play a pivotal role in the maintenance of maternal immune tolerance. Using a murine allogeneic pregnancy model in the current study, we identified the up-regulation of gonadotropin-releasing hormone receptor (GnRHR) in decidual T cell subsets including CD4 conventional T cells, CD8 T cells, and CD4Foxp3 Tregs. Read More

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Qiyusanlong Formula Induces Autophagy in Non-Small-Cell Lung Cancer Cells and Xenografts through the mTOR Signaling Pathway.

Evid Based Complement Alternat Med 2021 22;2021:5575453. Epub 2021 May 22.

Institute of Traditional Chinese Medicine of Respiratory Disease Prevention, Anhui University of Chinese Medicine, Hefei, Anhui, China.

Objective: Qiyusanlong (QYSL) formula has been used in the clinic for more than 20 years and has been proved to have pronounced efficacy in the treatment of non-small-cell lung cancer (NSCLC). This work aims to evaluate the molecular mechanism of QYSL formula action on NSCLC, specifically in relation to autophagy induction.

Methods: In vitro, CCK-8 was used to detect the effect of QYSL serum on cell viability in A549 cells. Read More

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Timosaponin BII improved osteoporosis caused by hyperglycemia through promoting autophagy of osteoblasts via suppressing the mTOR/NFκB signaling pathway.

Free Radic Biol Med 2021 08 13;171:112-123. Epub 2021 May 13.

Spine Disease Research Institute, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, 200032, China. Electronic address:

Defective autophagy occurred in osteoblasts under stress induced by high glucose and played an essential role in the development of diabetic osteoporosis. Timosaponin BII, a steroidal saponin isolated from the rhizomes of Anemarrhena asphodeloides Bunge, possessed anti-osteoporosis properties. In this study, we investigated the efficacy and mechanism of timosaponin BII on diabetic osteoporosis. Read More

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The Efficacy and Safety of the mTOR Signaling Pathway Activator, MHY1485, for Activation of Human Ovarian Tissue.

Front Genet 2020 4;11:603683. Epub 2021 Feb 4.

Shanghai Key Laboratory of Embryo Original Diseases, International Peace Maternity and Child Health Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.

Background: Premature ovarian insufficiency (POI) is characterized by abnormal ovarian function before the age of 40. POI showed that primordial follicles developed in disorder. mTOR signaling plays a vital role in the process of follicle development. Read More

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February 2021

Fumonisin B1 induces nephrotoxicity via autophagy mediated by mTORC1 instead of mTORC2 in human renal tubule epithelial cells.

Food Chem Toxicol 2021 Mar 3;149:112037. Epub 2021 Feb 3.

College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu Province, 210095, China; Institute of Nutritional and Metabolic Disorders in Domestic Animals and Fowls, Nanjing Agricultural University, Nanjing, Jiangsu Province, 210095, China; MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu Province, 210095, China. Electronic address:

Fumonisin B1 (FB1), a worldwide contaminating mycotoxin, can cause global food issue. It has been reported that FB1 is related to chronic kidney disease of unknown etiology. However, the study of FB1-induced nephrotoxicity in vitro is very limited and the mechanism is unknown. Read More

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An aqueous extract of Prunella vulgaris L. inhibits the growth of papillary thyroid carcinoma by inducing autophagy in vivo and in vitro.

Phytother Res 2021 Jan 13. Epub 2021 Jan 13.

Food Safety and Health Research Center, Guangdong Provincial Key Laboratory of Tropical Disease Research, School of Public Health, Southern Medical University, Guangzhou, China.

The continued global rise in papillary thyroid carcinoma (PTC) combined with potential adverse effects of regular treatments calls for an alternative therapy. Prunella vulgaris L. (PV) is commonly used as a herbal remedy for thyroid diseases in China, but its influence on PTC is unclear. Read More

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January 2021

Rapamycin alleviates cognitive impairment in murine vascular dementia: The enhancement of mitophagy by PI3K/AKT/mTOR axis.

Tissue Cell 2021 Apr 25;69:101481. Epub 2020 Dec 25.

Department of Neurology, Hebei Medical University, China; Department of Neurology, HeBei General Hospital, China. Electronic address:

There are no approved symptomatic treatments for vascular dementia (VaD). Rapamycin (RAPA) improves cognitive deficits in Alzheimer's disease rats. To explore whether RAPA improves cognitive impairment after VaD and its possible molecular mechanisms. Read More

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Melatonin regulates chicken granulosa cell proliferation and apoptosis by activating the mTOR signaling pathway via its receptors.

Poult Sci 2020 Nov 26;99(11):6147-6162. Epub 2020 Aug 26.

College of Animal Science and Technology, Hebei Agricultural University, Baoding Hebei 071001, China.

Melatonin is a key regulator of follicle granular cell maturation and ovulation. The mammalian target of rapamycin (mTOR) pathway plays an important role in cell growth regulation. Therefore, our aim was to investigate whether the mTOR signaling pathway is involved in the regulation of melatonin-mediated proliferation and apoptotic mechanisms in granulosa cells. Read More

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November 2020

Inhibition of human immunodeficiency virus type 1 by niclosamide through mTORC1 inhibition.

Heliyon 2020 Jun 3;6(6):e04050. Epub 2020 Jun 3.

Department of Microbiology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.

Niclosamide has been known to inhibit a number of pH-dependent viruses via the neutralization of endosomal acidic pH. It has also been shown to disrupt the mTORC1 signaling pathway. The replication of many viruses requires mTORC1 activation. Read More

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Target of rapamycin signaling inhibits autophagy in sea cucumber Apostichopus japonicus.

Fish Shellfish Immunol 2020 Jul 11;102:480-488. Epub 2020 May 11.

School of Ocean, Yantai University, Yantai, 264005, PR China.

Autophagy mediated by mTOR pathway is a particularly important immune defense mechanism in the pathogens infected mammals. However, the role of TOR in echinoderm autophagy is largely unknown. Here, a cDNA encoding TOR protein was cloned and characterized from sea cucumber Apostichopus japonicus (designated as AjTOR) and its biological functions were also investigated. Read More

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The in vitro effects of phospholipase D1-mTOR axis in liver fibrogenesis.

Life Sci 2020 Jun 30;251:117595. Epub 2020 Mar 30.

Department of Gastroenterology and Hepatology, Institution of Digestive Diseases, Tongji Hospital, Tongji University School of Medicine, Shanghai, China. Electronic address:

Aims: The activation of hepatic stellate cells (HSCs) plays a central role in liver fibrosis progression. Phospholipase D (PLD) enzymes participate in multiple cellular activities. However, whether and how PLD regulates HSCs activation remain elusive. Read More

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STYX/FBXW7 axis participates in the development of endometrial cancer cell via Notch-mTOR signaling pathway.

Biosci Rep 2020 04;40(4)

Department of Obstetrics, Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing 100026, China.

Endometrial cancer (EC) is the most common gynecologic malignancy in world. It has been reported that the mutation rate of FBXW7 is frequent in EC, but the specific functions of FBXW7 remain unknown in EC. In the present study, we revealed the role and mechanism of FBXW7 in EC cells. Read More

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Molecular hydrogen attenuates sepsis-induced neuroinflammation through regulation of microglia polarization through an mTOR-autophagy-dependent pathway.

Int Immunopharmacol 2020 Apr 10;81:106287. Epub 2020 Feb 10.

Department of Anesthesiology, The Second Hospital of Tianjin Medical University, Tianjin 300211, China.

Sepsis-associated encephalopathy (SAE) is the cognitive impairment resulting from sepsis and is associated with increased morbidity and mortality. Hydrogen has emerged as a promising therapeutic agent to alleviate SAE. The mechanism, however, remains unclear. Read More

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Resveratrol-induced brown fat-like phenotype in 3T3-L1 adipocytes partly via mTOR pathway.

Food Nutr Res 2020 14;64. Epub 2020 Jan 14.

Department of Nutrition, School of Public Health, Sun Yat-sen University, Guangzhou, China.

Background: Browning of white adipose tissues (WAT) is recognized as a novel way to combat obesity and its related comorbidities. Thus, a lot of dietary agents contributing to browning of WAT have been identified.

Objective: In this study, we try to explore the mechanism of the browning of WAT induced by resveratrol (Res) in 3T3-L1 adipocytes. Read More

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January 2020

Scoparone improves hepatic inflammation and autophagy in mice with nonalcoholic steatohepatitis by regulating the ROS/P38/Nrf2 axis and PI3K/AKT/mTOR pathway in macrophages.

Biomed Pharmacother 2020 May 28;125:109895. Epub 2020 Jan 28.

Department of Gastroenterology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China. Electronic address:

Background And Aims: Scoparone has been shown to ameliorate many forms of liver disease, and several underlying molecular mechanisms involved have been previously revealed. However, the potential role of scoparone in autophagy, which is dysregulated in nonalcoholic fatty liver disease-nonalcoholic steatohepatitis (NAFLD-NASH), has not been evaluated. In the current study, we investigated the effect and potential mechanisms of scoparone in hepatic autophagy in mice with NASH. Read More

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Adiponectin inhibits the differentiation and maturation of osteoclasts via the mTOR pathway in multiple myeloma.

Int J Mol Med 2020 Apr 24;45(4):1112-1120. Epub 2020 Jan 24.

Department of Hematology, Tianjin Medical University General Hospital, Tianjin 300052, P.R. China.

The present study sought to investigate the correlation between adipose cytokines (visfatin, leptin and adiponectin) and markers of multiple myeloma bone disease, and to determine the effects and mechanism of action of adiponectin on the differentiation and maturation of osteoclasts in multiple myeloma (MM). The levels of visfatin, leptin and adiponectin were measured. Their association with the indices of myeloma tumor load and bone disease were analyzed. Read More

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CXCR2 Ligands and mTOR Activation Enhance Reprogramming of Human Somatic Cells to Pluripotent Stem Cells.

Stem Cells Dev 2020 02 6;29(3):119-132. Epub 2020 Jan 6.

Institute of Stem Cell Research, Korea University College of Medicine, Seoul, Korea.

Induced pluripotent stem cell (iPSC) technology has great promise in regenerative medicine and disease modeling. In this study, we show that human placenta-derived cell conditioned medium stimulates chemokine (C-X-C motif) receptor 2 (CXCR2) in human somatic cells ectopically expressing the pluripotency-associated transcription factors , , , and (OSKM), leading to mechanistic target of rapamycin (mTOR) activation. This causes an increase in endogenous cMYC levels and a decrease in autophagy, thereby enhancing the reprogramming efficiency of human somatic cells into iPSCs. Read More

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February 2020

Monotropein attenuates oxidative stress via Akt/mTOR-mediated autophagy in osteoblast cells.

Biomed Pharmacother 2020 Jan 4;121:109566. Epub 2019 Nov 4.

School of Pharmacy, Second Military Medical University, Shanghai, 200433, China. Electronic address:

Oxidative stress is a crucial pathogenic factor in osteoporosis. Autophagy is a cellular self-digestion process that can selectively remove damaged organelles under oxidative stress, and thus presents a potential therapeutic target against osteoporosis. Monotropein is an iridoid glycoside which can increase osteoblastic bone formation and be applied for medicinal purpose in China. Read More

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January 2020

Trichostatin A modulates the macrophage phenotype by enhancing autophagy to reduce inflammation during polymicrobial sepsis.

Int Immunopharmacol 2019 Dec 31;77:105973. Epub 2019 Oct 31.

Department of Critical Care Medicine, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China; Institute of Anesthesiology and Critical Care Medicine, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China. Electronic address:

Sepsis is a syndrome of life-threatening organ dysfunction caused by dysregulated host responses to infection. Macrophage polarization is a key process involved in the pathogenesis of sepsis. Recent evidence has demonstrated that autophagy participates in the regulation of macrophage polarization in different phases of inflammation. Read More

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December 2019

Homocysteine enhances neural stem cell autophagy in in vivo and in vitro model of ischemic stroke.

Cell Death Dis 2019 07 22;10(8):561. Epub 2019 Jul 22.

Department of Nutrition and Food Science, School of Public Health, Tianjin Medical University, Tianjin, China.

The elevated level of the amino acid metabolite homocysteine (Hcy) is known as a risk factor for ischemic stroke. The molecular mechanisms responsible for neurotoxicity of Hcy remain largely unknown in ischemic brains. The previous studies have shown that Hcy decreases the proliferation and viability of neural stem cells (NSCs) in vivo and in vitro. Read More

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Melatonin suppresses milk fat synthesis by inhibiting the mTOR signaling pathway via the MT1 receptor in bovine mammary epithelial cells.

J Pineal Res 2019 Oct 23;67(3):e12593. Epub 2019 Jul 23.

College of Animal Science and Technology, Northwest A&F University, Yangling, China.

Milk fat content is an important criterion for assessing milk quality and is one of the main target traits of dairy cattle breeding. Recent studies have shown the importance of melatonin in regulating lipid metabolism, but the potential effects of melatonin on milk fat synthesis in bovine mammary epithelial cells (BMECs) remain unclear. Here, we showed that melatonin supplementation at 10 μmol/L significantly downregulated the mRNA expression of lipid metabolism-related genes and resulted in lower lipid droplet formation and triglyceride accumulation. Read More

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October 2019

The Glp-1 Analog Liraglutide Protects Against Angiotensin II and Pressure Overload-Induced Cardiac Hypertrophy via PI3K/Akt1 and AMPKa Signaling.

Front Pharmacol 2019 5;10:537. Epub 2019 Jun 5.

Department of Endocrinology, First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.

The Glp-1 analog, liraglutide (Lir), has been shown to reduce infarct size and improve cardiac function after myocardial ischemia in rodents with or without diabetes. However, the effect of Lir on angiotensin II (AngII) and pressure overload induced cardiac hypertrophy in nondiabetic mice and the underlying mechanisms are unclear. The aim of this study was to investigate the effect of Lir on cardiac hypertrophy induced by AngII infusion and pressure overload and to explore its possible mechanism. Read More

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Targeting β3-adrenergic receptor signaling inhibits neuroblastoma cell growth via suppressing the mTOR pathway.

Biochem Biophys Res Commun 2019 06 25;514(1):295-300. Epub 2019 Apr 25.

Program of Molecular Medicine, Department of Internal Medicine, Affiliated Guangzhou Women and Childrens' Medical Center, Zhongshan School of Medicine, Sun Yat-Sen University, Guangzhou, China; Department of Biochemistry, Zhongshan School of Medicine, Sun Yat-Sen University, Guangzhou, China; Guangdong Engineering & Technology Research Center for Gene Manipulation and Biomacromolecular Products (Sun Yat-Sen University), Guangzhou, China. Electronic address:

Neuroblastoma (NB), the most common extracranial solid tumor in childhood, always leads to an unfavorable prognosis. β3-adrenergic receptor (β3-AR) signaling plays an important role in lipid metabolism. Although previous studies have focused mainly on the role of β2-AR in tumor cells; there are few studies about the cancer-related function of β3-AR. Read More

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Apelin-36 exerts the cytoprotective effect against MPP-induced cytotoxicity in SH-SY5Y cells through PI3K/Akt/mTOR autophagy pathway.

Life Sci 2019 May 21;224:95-108. Epub 2019 Mar 21.

Neurobiology Institute, Jining Medical University, 272067 Jining, China. Electronic address:

Aims: Parkinson's disease (PD) is a common neurodegenerative disease typically associated with the accumulation of α-synuclein. Autophagy impairment is thought to be involved in the dopaminergic neurodegeneration in PD. We investigate the effect of Apelin-36 on the activated phosphatidylinositol 3-kinase (PI3K)/protein kinase B(Akt)/the mammalian target of rapamycin (mTOR) autophagy pathway in 1-methyl-4-phenylpyridinium (MPP)-treated SH-SY5Y cells, which is involved in the cytoprotective effect of Apelin-36. Read More

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